Liquid chromatography analyzer and sample analysis system

Abstract

The invention is applicable to the field of sample analysis, and discloses a liquid chromatographic analyzer and a sample analysis system.The liquid chromatographic analyzer comprises a sample supply assembly, a liquid phase fluid supply assembly, a first switching component, a sample liquid preparation channel, a chromatographic column, a detector and a first controller. The sample supply assembly includes a first filter that is cleaned with a first cleaning reagent in the chromatography mode and with a second cleaning reagent different from the first cleaning reagent in the first maintenance mode, the first filter is cleaned by adding cleaning reagents with different components so as to prolong the service life of the first filter, so that the frequency of replacing the first filter by a user is reduced.

Description

Technical Field

[0001] The present invention relates to the field of sample analysis, and in particular to a liquid chromatography analyzer and a sample analysis system having the liquid chromatography analyzer. Background Art

[0002] In a liquid chromatography analyzer provided by related technologies, in order to protect the chromatographic column and avoid an increase in the pressure of the liquid path system or damage to the chromatographic column caused by blockage of the chromatographic column, a filter is provided between the chromatographic column and the switching member to filter the sample liquid and the liquid phase fluid flowing to the chromatographic column. The main interception objects of this filter include: cell debris, lipid particles, white blood cells, platelets, fibrin, and impurities in the liquid phase fluid or the sample (such as debris of the sample container, hair, etc.). When these interception objects accumulate in the filter, it is easy to cause the filter to become blocked, resulting in impurities in the sample, such as debris and hair of the sample container, entering the high-pressure liquid path system and blocking the high-pressure channel (generally with a diameter of 0.1 mm - 0.25 mm), thereby causing the system pressure to increase and requiring shutdown for maintenance.

[0003] In order to avoid the occurrence of the phenomenon that the pressure of the liquid path system increases due to blockage of the filter, the solutions adopted in related technologies are roughly two types: The first solution is to frequently replace the new filter; the second solution is to regularly clean the filter with a cleaning liquid. However, both of these solutions still have deficiencies, which are specifically analyzed as follows:

[0004] (1) In the first solution, frequently replacing the filter will, on the one hand, take up a lot of time of the operator, and on the other hand, since this filter is a high-pressure filter and is relatively expensive, frequently replacing this filter will result in a relatively high usage cost of the liquid chromatography analyzer.

[0005] (2) In the second solution, when cleaning the filter, the direction of the fluid flowing through the filter is the same as the direction of the fluid flowing through the filter during the execution of the chromatographic analysis project, and it is difficult to wash away the relatively large impurities. Moreover, the cleaning liquid used to regularly clean the filter in related technologies, due to its single component, is difficult to decompose and clean the relatively large impurities on the filter. Therefore, the cleaning effect of the filter in related technologies is not very good, and it is also easy to have the problem that the filter becomes blocked and needs to be replaced by shutting down the machine. Summary of the Invention

[0006] The first object of the present invention is to provide a liquid chromatography analyzer, which aims to solve the technical problem that the usage cost of the liquid chromatography analyzer is high due to the need to frequently replace the high-pressure filter in the liquid chromatography analyzer.

[0007] To achieve the above object, the solution provided by the present invention is: A liquid chromatography analyzer, comprising:

[0008] A sample supply assembly, a liquid-phase fluid supply assembly, a first switching member, a sample liquid preparation channel, a chromatography column, a detector, and a first controller. The first switching member has a switchable first connection state and a second connection state. In the first connection state, the first switching member connects the sample supply assembly, the sample liquid preparation channel, and connects the liquid-phase fluid supply assembly and the chromatography column. In the second connection state, the first switching member connects the liquid-phase fluid supply assembly, the sample liquid preparation channel, and the chromatography column. The chromatography column is disposed between the first switching member and the detector;

[0009] The sample supply assembly includes a reaction container, a sample dispensing device, and a first liquid path assembly. The reaction container is used to provide a reaction site for the sample and the reaction reagent to prepare a sample liquid. The sample dispensing device is used to aspirate the sample from the sample container and dispense at least part of the aspirated sample into the reaction container. The first liquid path assembly is used to drive the sample liquid in the reaction container to be transported to the sample liquid preparation channel through the first switching member when the first switching member is in the first connection state. The first liquid path assembly includes a first filter, and the first filter is disposed between the reaction container and the first switching member to filter the sample liquid transported from the reaction container to the sample liquid preparation channel. The first liquid path assembly is also used to drive a first cleaning reagent to clean the first filter, and to drive a second cleaning reagent to clean the first filter;

[0010] The liquid-phase fluid supply assembly is used for: when the first switching member is in the second connection state, driving the sample liquid in the sample liquid preparation channel to be transported to the chromatography column by the liquid-phase fluid, and when the first switching member is in the first connection state, driving the liquid-phase fluid to flow through the first switching member and the chromatography column in sequence;

[0011] The chromatography column is used to adsorb the sample liquid and to allow the liquid-phase fluid to elute the sample liquid to form a test liquid;

[0012] The detector is used to perform chromatographic analysis on the test liquid flowing out of the chromatography column;

[0013] The first controller is configured with a chromatographic analysis mode and a first maintenance mode;

[0014] In the chromatographic analysis mode, the first controller is configured to perform the following actions: when the first switching member is in the first communication state, control the first liquid path assembly to drive the sample liquid to flow from the reaction container along a first direction through the first filter and be delivered to the sample liquid preparation channel through the first switching member; after the sample liquid is delivered to the sample liquid preparation channel, control the first liquid path assembly to drive the first cleaning reagent to flow through the first filter along a second direction to backwash the first filter with the first cleaning reagent, where the first direction and the second direction are two opposite directions;

[0015] In the first maintenance mode, the first controller is configured to perform the following actions: control the first liquid path assembly to drive the second cleaning reagent to flow through the first filter to clean the first filter;

[0016] The first cleaning reagent and the second cleaning reagent are two reagents with different components.

[0017] As an implementation manner, the pH value of the first cleaning reagent is less than the pH value of the second cleaning reagent.

[0018] As an implementation manner, the second cleaning reagent is an alkaline reagent or a protease reagent.

[0019] As an implementation manner, the second cleaning reagent is an alkaline reagent, and the pH value of the second cleaning reagent is greater than or equal to 9 and less than or equal to 14.

[0020] As an implementation manner, the first cleaning reagent and the reaction reagent are the same reagent; and / or,

[0021] The sample is a blood sample, and the reaction reagent is a hemolytic agent containing a surfactant.

[0022] As an implementation manner, the sample is a blood sample, the reaction reagent is a hemolytic agent, and the hemolytic agent is used to dissolve red blood cells in the blood sample into red blood cell fragments with a length less than or equal to 1 μm; and / or,

[0023] In the reaction liquid obtained by reacting the sample with the reaction reagent, the proportion of the number of particles with a size of 0 μm to 1 μm in the total number of particles is 40% to 90%, and the proportion of the number of particles with a size of 1 μm to 2 μm in the total number of particles is 10% to 60%.

[0024] As an implementation manner, the liquid chromatography analyzer prestores at least one of the following first preset conditions: reaching a first preset duration since the last execution of the first maintenance mode; the cumulative number of times of performing chromatographic analysis items by the liquid chromatography analyzer since the last execution of the first maintenance mode reaching a first preset number; reaching a first preset time point of a first preset period; obtaining information that the first filter needs to be cleaned and maintained according to an operation instruction received by the human-machine interaction device;

[0025] The first controller is further configured to: when any one of the first preset conditions is satisfied, execute the first maintenance mode or output information prompting to execute the first maintenance mode.

[0026] As an implementation manner, the sample dispensing device includes a sample needle, and the sample needle is used to suck the sample from the sample container at the liquid suction level and dispense at least part of the sucked sample into the reaction container and is used to suck the second cleaning reagent from the cleaning reagent container at the liquid suction level and dispense the sucked second cleaning reagent into the reaction container;

[0027] In the first maintenance mode, the first controller is further configured to: before controlling the first liquid path component to drive the second cleaning reagent to flow through the first filter to clean the first filter, control the sample dispensing device to suck the second cleaning reagent from the cleaning reagent container at the liquid suction level and dispense it into the reaction container;

[0028] Controlling the first liquid path component to drive the second cleaning reagent to flow through the first filter includes: controlling the first liquid path component to drive the second cleaning reagent to flow through the first filter from the reaction container along the first direction.

[0029] As an implementation manner, the liquid chromatography analyzer further includes a cleaning reagent placing component and a first transmission component, the cleaning reagent placing component is used for the cleaning reagent container loaded with the second cleaning reagent to be placed, and the first transmission component is used to transmit the cleaning reagent container loaded with the second cleaning reagent from the cleaning reagent placing component to the liquid suction level;

[0030] In the first maintenance mode, the first controller is further configured to: before controlling the sample dispensing device to suck the second cleaning reagent from the cleaning reagent container at the liquid suction level and dispense it into the reaction container, control the first transmission component to transmit the cleaning reagent container loaded with the second cleaning reagent from the cleaning reagent placing component to the liquid suction level.

[0031] As an implementation manner, the first liquid path component further includes a first suction and discharge driving device, a second switching member, a third switching member, and a liquid suction pipeline. The liquid suction pipeline is used to suck the reaction reagent from the reaction reagent container loaded with the reaction reagent. The first suction and discharge driving device can be switchably connected to the liquid suction pipeline and the third switching member through the second switching member. The second switching member can be switchably connected to the reaction container and the first switching member through the third switching member. The first filter is disposed between the reaction container and the third switching member; and / or,

[0032] The liquid chromatography analyzer further includes a waste liquid channel. In the first connection state, the first switching member connects the sample supply component, the sample liquid preparation channel, and the waste liquid channel, and connects the liquid phase fluid supply component and the chromatography column. In the second connection state, the first switching member connects the liquid phase fluid supply component, the sample liquid preparation channel, and the chromatography column, and connects the sample supply component and the waste liquid channel. Controlling the first liquid path component to drive the second cleaning reagent to flow through the first filter includes: controlling the first liquid path component to drive the second cleaning reagent to flow through the first filter along the first direction to clean the first filter and conveying it to the waste liquid channel through the first switching member.

[0033] As an implementation manner, the liquid chromatography analyzer further includes a second filter, and the second filter is disposed between the first switching member and the chromatography column;

[0034] The filtration accuracy of the second filter is greater than that of the first filter.

[0035] As an implementation manner, the second filter includes a first filter layer and a second filter layer. The first filter layer is disposed between the first switching member and the second filter layer. The aperture of the filter holes of the first filter layer is in the range of 0.5 μm to 20 μm, and the aperture of the filter holes of the second filter layer is in the range of 0.2 μm to 5 μm; and / or,

[0036] The aperture of the filter holes of the first filter is in the range of 10 μm to 200 μm.

[0037] As an implementation manner, the liquid chromatography analyzer prestores preset replacement conditions, and the first controller is further configured to: when the chromatography column meets the preset replacement conditions, output a warning signal to prompt to replace the chromatography column and the second filter simultaneously.

[0038] As an implementation manner, the liquid chromatography analyzer further includes a fourth switching member, and the second filter is switchably connected to the chromatography column and the waste liquid channel through the fourth switching member;

[0039] The first controller is further configured with a second maintenance mode;

[0040] In the second maintenance mode, the first controller is configured to perform the following actions: when the first switching member is in the first connection state, control the first liquid path assembly to drive the second cleaning reagent to be transported to the sample liquid preparation channel through the first switching member, control the first switching member to switch to the second connection state, and control the second liquid path assembly to drive the second cleaning reagent in the sample liquid preparation channel to flow through the second filter, the fourth switching member, and the waste liquid channel in sequence, so as to clean the second filter with the second cleaning reagent;

[0041] Preferably, the liquid chromatography analyzer prestores at least one of the following second preset conditions: reaching a second preset duration since the last execution of the second maintenance mode; the cumulative number of times the liquid chromatography analyzer has executed chromatographic analysis items since the last execution of the second maintenance mode reaches a second preset number; reaching a second preset time point of a second preset period; obtaining information that the second filter needs to be cleaned and maintained according to an operation instruction received by the human-computer interaction device; the first controller is further configured to: when any one of the second preset conditions is satisfied, execute the second maintenance mode or output information prompting to execute the second maintenance mode.

[0042] The second object of the present invention is to provide a liquid chromatography analyzer, including:

[0043] A sample supply assembly, a liquid phase fluid supply assembly, a first switching member, a sample liquid preparation channel, a chromatography column, a detector, and a first controller. The first switching member has a switchable first connection state and a second connection state. In the first connection state, the first switching member connects the sample supply assembly, the sample liquid preparation channel, and connects the liquid phase fluid supply assembly and the chromatography column. In the second connection state, the first switching member connects the liquid phase fluid supply assembly, the sample liquid preparation channel, and the chromatography column. The chromatography column is arranged between the first switching member and the detector;

[0044] The sample supply component includes a reaction vessel, a sample dispensing device, and a first liquid path component. The reaction vessel is used to provide a reaction site for the sample and the reaction reagent to form a sample solution. The sample dispensing device is used to aspirate the sample from the sample container and dispense at least a part of the aspirated sample into the reaction vessel. The first liquid path component is used to drive the sample solution in the reaction vessel to be transported to the sample solution preparation channel through the first switching member when the first switching member is in the first connection state. The first liquid path component includes a first filter, and the first filter is arranged between the reaction vessel and the first switching member to filter the sample solution transported from the reaction vessel to the sample solution preparation channel. The first liquid path component is also used to drive a second cleaning reagent to clean the first filter;

[0045] The liquid-phase fluid supply component is used for: when the first switching member is in the second connection state, driving the sample solution in the sample solution preparation channel to be transported to the chromatography column through the liquid-phase fluid, and when the first switching member is in the second connection state, driving the liquid-phase fluid to flow through the first switching member and the chromatography column in sequence;

[0046] The chromatography column is used to adsorb the sample solution and to allow the liquid-phase fluid to elute the sample solution to form a test solution;

[0047] The detector is used to perform chromatographic analysis on the test solution flowing out of the chromatography column;

[0048] The first controller is configured with a chromatographic analysis mode and a first maintenance mode;

[0049] In the chromatographic analysis mode, the first controller is configured to perform the following actions: when the first switching member is in the first connection state, control the first liquid path component to drive the sample solution to flow from the reaction vessel through the first filter and be transported to the sample solution preparation channel through the first switching member, control the first switching member to switch to the second connection state, control the second liquid path component to drive the sample solution in the sample solution preparation channel to be transported to the chromatography column through the liquid-phase fluid, control the first switching member to switch to the first connection state, control the second liquid path component to drive the liquid-phase fluid to be transported to the chromatography column through the first switching member to elute the sample solution, and control the detector to perform chromatographic analysis on the test solution flowing out of the chromatography column;

[0050] In the first maintenance mode, the first controller is configured to perform the following actions: control the first liquid path component to drive the second cleaning reagent to flow through the first filter to clean the first filter;

[0051] Wherein, the second cleaning reagent is an alkaline reagent or a protease reagent.

[0052] As an implementation manner, the sample dispensing device includes a sample needle, which is used to aspirate the sample from the sample container located at the aspiration level and dispense at least a part of the aspirated sample into the reaction container, and is also used to aspirate the second cleaning reagent from the cleaning reagent container located at the aspiration level and dispense the aspirated second cleaning reagent into the reaction container;

[0053] In the first maintenance mode, the first controller is further configured to: before controlling the first liquid path component to drive the second cleaning reagent to flow through the first filter to clean the first filter, control the sample dispensing device to aspirate the second cleaning reagent from the cleaning reagent container located at the aspiration level and dispense it into the reaction container;

[0054] Controlling the first liquid path component to drive the second cleaning reagent to flow through the first filter includes: controlling the first liquid path component to drive the second cleaning reagent to flow through the first filter from the reaction container along the first direction;

[0055] Preferably, the liquid chromatography analyzer further includes a cleaning reagent placement component and a first transmission component. The cleaning reagent placement component is used to place the cleaning reagent container loaded with the second cleaning reagent, and the first transmission component is used to transmit the cleaning reagent container loaded with the second cleaning reagent from the cleaning reagent placement component to the aspiration level. In the first maintenance mode, the first controller is further configured to: before controlling the sample dispensing device to aspirate the second cleaning reagent from the cleaning reagent container located at the aspiration level and dispense it into the reaction container, control the first transmission component to transmit the cleaning reagent container loaded with the second cleaning reagent from the cleaning reagent placement component to the aspiration level.

[0056] The third object of the present invention is to provide a liquid chromatography analyzer, including:

[0057] A sample supply component, a liquid phase fluid supply component, a first switching member, a sample liquid preparation channel, a second filter, a chromatography column, a detector, and a first controller. The first switching member has a switchable first communication state and a second communication state. In the first communication state, the first switching member connects the sample supply component, the sample liquid preparation channel, and also connects the liquid phase fluid supply component and the second filter. In the second communication state, the first switching member connects the liquid phase fluid supply component, the sample liquid preparation channel, and the second filter. The chromatography column is arranged between the second filter and the detector;

[0058] The sample supply assembly includes a reaction vessel, a sample dispensing device, and a first liquid path assembly. The reaction vessel is used to provide a reaction site for the sample and the reaction reagent to form a sample solution. The sample dispensing device is used to aspirate the sample from the sample container and dispense at least a portion of the aspirated sample into the reaction vessel. The first liquid path assembly distributes the reaction reagent to the reaction vessel and is used to drive the sample solution in the reaction vessel to be transported to the sample solution preparation channel through the first switching member when the first switching member is in the first connection state;

[0059] The liquid phase fluid supply assembly is used for: when the first switching member is in the second connection state, driving the sample solution in the sample solution preparation channel to be transported to the chromatography column through the second filter by the liquid phase fluid, and when the first switching member is in the first connection state, driving the liquid phase fluid to flow through the first switching member, the second filter, and the chromatography column in sequence;

[0060] The chromatography column is used for adsorbing the sample solution and for the liquid phase fluid to elute the sample solution to form a test solution;

[0061] The detector is used for performing chromatographic analysis on the test solution flowing out of the chromatography column;

[0062] The first controller is configured with a chromatographic analysis mode;

[0063] In the chromatographic analysis mode, the first controller is configured to perform the following actions: when the first switching member is in the first connection state, controlling the first liquid path assembly to distribute the reaction reagent to the reaction vessel, controlling the sample dispensing device to distribute the sample to the reaction vessel, controlling the first switching member to switch to the second connection state, controlling the second liquid path assembly to drive the sample solution in the sample solution preparation channel to be transported to the chromatography column through the second filter by the liquid phase fluid, controlling the first switching member to switch to the first connection state, controlling the second liquid path assembly to drive the liquid phase fluid to be transported to the chromatography column through the first switching member and the second filter to elute the sample solution, and controlling the detector to perform chromatographic analysis on the test solution flowing out of the chromatography column;

[0064] Wherein, the reaction reagent is a hemolytic agent containing a surfactant.

[0065] The fourth object of the present invention is to provide a liquid chromatography analyzer, including:

[0066] A sample supply component, a liquid-phase fluid supply component, a first switching member, a sample liquid preparation channel, a second filter, a chromatography column, a detector and a first controller, wherein the first switching member has a switchable first connection state and a second connection state, in which the first switching member connects the sample supply component, the sample liquid preparation channel and the liquid-phase fluid supply component and the second filter, and in which the first switching member connects the liquid-phase fluid supply component, the sample liquid preparation channel and the second filter, and the chromatography column is disposed between the second filter and the detector;

[0067] The sample supply assembly includes a reaction container, a sample dispensing device and a first liquid circuit assembly, wherein the reaction container is used to provide a reaction site for the sample and the reaction reagent to form a sample liquid, the sample dispensing device is used to absorb the sample from the sample container and dispense at least part of the absorbed sample into the reaction container, the first liquid circuit assembly distributes the reaction reagent to the reaction container and drives the sample liquid in the reaction container to be transported to the sample liquid preparation channel through the first switching member when the first switching member is in the first connected state;

[0068] The liquid-phase fluid supply assembly is used to: when the first switching member is in the second communication state, drive the sample liquid in the sample liquid preparation channel to be transported to the chromatography column through the second filter by means of the liquid-phase fluid; and when the first switching member is in the first communication state, drive the liquid-phase fluid to flow through the first switching member, the second filter and the chromatography column in sequence;

[0069] The chromatography column is used to adsorb the sample liquid, and is used for the liquid phase fluid to elute the sample liquid to form a liquid to be tested;

[0070] The detector is used to perform chromatographic analysis on the liquid to be tested flowing out of the chromatography column;

[0071] The first controller is configured with a chromatographic analysis mode;

[0072] In the chromatographic analysis mode, the first controller is configured to perform the following actions: when the first switching member is in the first communication state, control the first liquid path assembly to distribute a reaction reagent to the reaction vessel, control the sample dispensing device to distribute a sample to the reaction vessel, control the first switching member to switch to the second communication state, control the second liquid path assembly to drive the sample liquid in the sample liquid preparation channel through a liquid phase fluid to be delivered to the chromatography column via the second filter, control the first switching member to switch to the first communication state, control the second liquid path assembly to drive the liquid phase fluid to be delivered to the chromatography column via the first switching member and the second filter to elute the sample liquid, and control the detector to perform chromatographic analysis on the test liquid flowing out of the chromatography column;

[0073] Among them, in the reaction liquid obtained by the reaction of the reaction reagent and the sample, the proportion of the number of particles with a size of 0 μm to 1 μm in the total number of particles is 40% to 90%, and the proportion of the number of particles with a size of 1 μm to 2 μm in the total number of particles is 10% to 60%.

[0074] The fifth object of the present invention is to provide a sample analyzer system, including:

[0075] A sample input device, which is at least used for placing a sample container loaded with a sample to achieve sample loading;

[0076] A liquid chromatography analyzer, which is used to suck a sample from the sample container and perform chromatographic analysis on at least part of the sucked sample;

[0077] A second transmission assembly, and the second transmission track is used to transmit the sample container from the sample input device to the liquid chromatography analyzer;

[0078] A second controller, which is used to control the second transmission assembly to transmit the sample container to the liquid chromatography analyzer;

[0079] Among them, the liquid chromatography analyzer includes a sample supply assembly, a liquid phase fluid supply assembly, a first switching member, a sample liquid preparation channel, a chromatography column, a detector, and a first controller. The first switching member has a switchable first communication state and a second communication state. In the first communication state, the first switching member connects the sample supply assembly, the sample liquid preparation channel, and connects the liquid phase fluid supply assembly and the chromatography column. In the second communication state, the first switching member connects the liquid phase fluid supply assembly, the sample liquid preparation channel, and the chromatography column. The chromatography column is disposed between the first switching member and the detector;

[0080] The sample supply assembly includes a reaction vessel, a sample dispensing device, and a first liquid path assembly. The reaction vessel is used to provide a reaction site for the sample and the reaction reagent to prepare a sample solution. The sample dispensing device is used to aspirate the sample from the sample container and dispense at least a part of the aspirated sample into the reaction vessel. The first liquid path assembly is used to drive the sample solution in the reaction vessel to be transported to the sample solution preparation channel through the first switching member when the first switching member is in the first connection state. The first liquid path assembly includes a first filter, and the first filter is arranged between the reaction vessel and the first switching member to filter the sample solution transported from the reaction vessel to the sample solution preparation channel. The first liquid path assembly is also used to drive a first cleaning reagent to clean the first filter and to drive a second cleaning reagent to clean the first filter;

[0081] The liquid-phase fluid supply assembly is used for: when the first switching member is in the second connection state, driving the sample solution in the sample solution preparation channel to be transported to the chromatography column through the liquid-phase fluid, and when the first switching member is in the first connection state, driving the liquid-phase fluid to flow through the first switching member and the chromatography column in sequence;

[0082] The chromatography column is used to adsorb the sample solution and to allow the liquid-phase fluid to elute the sample solution to form a test solution;

[0083] The detector is used to perform chromatographic analysis on the test solution flowing out of the chromatography column;

[0084] The first controller is configured with a chromatographic analysis mode and a first maintenance mode;

[0085] In the chromatographic analysis mode, the first controller is configured to perform the following actions: when the first switching member is in the first connection state, control the first liquid path assembly to drive the sample solution to flow from the reaction vessel along a first direction through the first filter and be transported to the sample solution preparation channel through the first switching member. After the sample solution is transported to the sample solution preparation channel, control the first liquid path assembly to drive the first cleaning reagent to flow through the first filter along a second direction to backwash the first filter with the first cleaning reagent, where the first direction and the second direction are two opposite directions;

[0086] In the first maintenance mode, the first controller is configured to perform the following actions: control the first liquid path assembly to drive the second cleaning reagent to flow through the first filter to clean the first filter;

[0087] The first cleaning reagent and the second cleaning reagent are two reagents with different components.

[0088] As an implementation manner, the sample dispensing device includes a sample needle, which is used to aspirate the sample from the sample container located at the aspiration level and dispense at least a part of the aspirated sample into the reaction container, and is also used to aspirate the second cleaning reagent from the cleaning reagent container located at the aspiration level and dispense the aspirated second cleaning reagent into the reaction container;

[0089] In the first maintenance mode, the first controller is further configured to: before controlling the first liquid path component to drive the second cleaning reagent to flow through the first filter to clean the first filter, control the sample dispensing device to aspirate the second cleaning reagent from the cleaning reagent container located at the aspiration level and dispense it into the reaction container;

[0090] Controlling the first liquid path component to drive the second cleaning reagent to flow through the first filter includes: controlling the first liquid path component to drive the second cleaning reagent to flow through the first filter from the reaction container along the first direction.

[0091] Preferably, the sample analysis system further includes a cleaning reagent loading component for loading the cleaning reagent container containing the second cleaning reagent, and the second transmission component is also used to transmit the cleaning reagent container containing the second cleaning reagent from the cleaning reagent loading component to the liquid chromatography analyzer; in the first maintenance mode, the first controller is further configured to: before controlling the sample dispensing device to aspirate the second cleaning reagent from the cleaning reagent container located at the aspiration level and dispense it into the reaction container, control the second transmission component to transmit the cleaning reagent container containing the second cleaning reagent from the cleaning reagent loading component to the liquid chromatography analyzer.

[0092] The liquid chromatography analyzer provided by the present invention is provided with a first filter in the first liquid path component of the sample supply component to filter the sample liquid conveyed from the reaction container to the sample liquid preparation channel, that is, a first filter is used to intercept impurities in the sample liquid in the low-pressure area. Since the first filter is a low-pressure filter, the price of the first filter is relatively low, and even the replacement cost is relatively low. In addition, in the chromatographic analysis mode of the present invention, the first cleaning reagent is driven by the first liquid path component to flow through the first filter in the second direction to backwash the first filter with the first cleaning reagent. In the first maintenance mode, the second cleaning reagent is driven by the first liquid path component to flow through the first filter to clean the first filter. That is, the present invention adopts two cleaning methods to regularly clean the first filter. Since the direction of the first cleaning reagent flowing through the first filter when the first cleaning reagent flushes the first filter is opposite to the direction of the fluid flowing through the first filter during the execution of the chromatographic analysis project, in this way, the relatively large-volume impurities on the first filter can be flushed away by the first cleaning reagent with a certain pressure. And different cleaning reagents with different components are used in the two cleaning methods of the first filter, and different components in the two cleaning reagents can be used to decompose the relatively large-volume impurities on the first filter respectively, so as to further reduce the occurrence of the phenomenon that the first filter is blocked, fully ensure the service life of the first filter, and further facilitate reducing the frequency of the operator replacing the first filter and reducing the use cost of the liquid chromatography analyzer. Since one cleaning method of the first filter can be carried out in the chromatographic analysis mode without special shutdown for cleaning, it is beneficial to ensure the cleanliness of the first filter and further reduce the frequency of the operator replacing the first filter by increasing the cleaning frequency of the first filter. BRIEF DESCRIPTION OF THE DRAWINGS

[0093] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the following drawings are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained based on the structures shown in these drawings.

[0094] Figure 1 is a three-dimensional schematic diagram of the liquid chromatography analyzer provided by the embodiment of the present invention;

[0095] Figure 2 is a liquid path schematic diagram of the liquid chromatography analyzer provided by the embodiment of the present invention when the first switching member is in the first connection state;

[0096] Figure 3 is a liquid path schematic diagram of the liquid chromatography analyzer provided by the embodiment of the present invention when the second switching member is in the first connection state;

[0097] Figure 4 It is a schematic diagram showing the connection of the liquid-phase fluid supply assembly provided by an embodiment of the present invention to a first container and a second container;

[0098] Figure 5 It is a schematic diagram of a sample analysis system provided by an embodiment of the present invention.

[0099] Explanation of the reference numerals in the drawings:

[0100] 100, liquid chromatography analyzer; 110, sample supply assembly; 111, reaction container; 112, sample dispensing device; 113, first liquid path assembly; 1131, first filter; 1132, first suction and discharge drive device; 1133, second switching member; 1134, third switching member; 1135, liquid suction pipeline; 120, liquid-phase fluid supply assembly; 121, first drive device; 122, second drive device; 123, mixing device; 130, first switching member; 140, sample liquid preparation channel; 150, chromatography column; 160, detector; 170, second filter; 180, fourth switching member; 190, waste liquid channel; 200, sample analysis system; 210, sample input device; 220, second transmission assembly; 300, first container; 400, second container; 500, reaction reagent container. Detailed implementation manners

[0101] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative efforts shall fall within the protection scope of the present invention.

[0102] It should be noted that all directional indications (such as up, down, left, right, front, back...) in the embodiments of the present invention are only used to explain the relative positional relationship and movement conditions between components in a specific posture. If the specific posture changes, the directional indications will also change accordingly.

[0103] It should also be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it can be directly on the other element or there may be an intermediate element at the same time. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or indirectly connected to the other element through an intermediate element.

[0104] In addition, the descriptions of "first", "second", etc. in the present invention are only used for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In addition, the technical solutions between the various embodiments can be combined with each other, but they must be based on the ability of ordinary technicians in the field to implement them. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such a combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0105] The liquid chromatography analyzer provided in the embodiment of the present invention is preferably a glycosylated hemoglobin analyzer, that is, the glycosylated hemoglobin analyzer provided in the embodiment of the present invention uses liquid chromatography to detect glycosylated hemoglobin.

[0106] Embodiment 1:

[0107] like Figures 1 to 4 As shown, a liquid chromatograph analyzer 100 provided in Embodiment 1 of the present invention includes a sample supply component 110, a liquid-phase fluid supply component 120, a first switching member 130, a sample liquid preparation channel 140, a chromatography column 150, a detector 160 and a first controller. The first switching member 130 has a switchable first connection state and a second connection state. In the first connection state, the first switching member 130 connects the sample supply component 110, the sample liquid preparation channel 140, and the liquid-phase fluid supply component 120 and the chromatography column 150. In the second connection state, the first switching member 130 connects the liquid-phase fluid supply component 120, the sample liquid preparation channel 140 and the chromatography column 150, and the chromatography column 150 is arranged between the first switching member 130 and the detector 160.

[0108] As an embodiment, the sample supply component 110 includes a reaction container 111, a sample dispensing device 112 and a first liquid circuit component 113. The reaction container 111 is used to provide a reaction field for the sample and the reaction reagent to form a sample liquid. The sample dispensing device 112 is used to absorb the sample from the sample container and dispense at least part of the absorbed sample into the reaction container 111. The first liquid circuit component 113 is used to drive the sample liquid in the reaction container 111 to be transported to the sample liquid preparation channel 140 through the first switching member 130 when the first switching member 130 is in a first connected state. The first liquid circuit component 113 includes a first filter 1131. The first filter 1131 is arranged between the reaction container 111 and the first switching member 130 to filter the sample liquid transported from the reaction container 111 to the sample liquid preparation channel 140. The first liquid circuit component 113 is also used to drive the first cleaning reagent to clean the first filter 1131, and to drive the second cleaning reagent to clean the first filter 1131.

[0109] The liquid-phase fluid supply assembly 120 is configured to: drive the sample liquid in the sample liquid preparation channel 140 to be transported to the chromatography column 150 through the liquid-phase fluid when the first switching member 130 is in the second communication state, and drive the liquid-phase fluid to flow through the first switching member 130 and the chromatography column 150 in sequence when the first switching member 130 is in the first communication state.

[0110] The chromatography column 150 is configured to adsorb the sample liquid and to allow the liquid-phase fluid to elute the sample liquid to form a liquid to be measured.

[0111] The detector 160 is configured to perform chromatographic analysis on the liquid to be measured flowing out of the chromatography column 150.

[0112] The first controller is configured with a chromatographic analysis mode and a first maintenance mode.

[0113] In the chromatographic analysis mode, the first controller is configured to perform the following actions: when the first switching member 130 is in the first communication state, control the first liquid path assembly 113 to drive the sample liquid to flow from the reaction container 111 along a first direction through the first filter 1131 and be transported to the sample liquid preparation channel 140 through the first switching member 130; after the sample liquid is transported to the sample liquid preparation channel 140, control the first liquid path assembly 113 to drive the first cleaning reagent to flow through the first filter 1131 along a second direction to backwash the first filter 1131 with the first cleaning reagent, where the first direction and the second direction are two opposite directions.

[0114] In the first maintenance mode, the first controller is configured to perform the following actions: control the first liquid path assembly 113 to drive the second cleaning reagent to flow through the first filter 1131 to clean the first filter 1131.

[0115] The first cleaning reagent and the second cleaning reagent are two reagents with different components. In the related art, the first filter 1131 is cleaned with a cleaning reagent of the same component, so that some residues in the first filter 1131 cannot be cleaned. In the embodiment of the present invention, in addition to using the first cleaning agent to perform backwashing cleaning of the first filter 1131 in the chromatographic analysis mode, a maintenance mode is added, and another cleaning reagent with a different component is used to clean the first filter 1131, which can clean the residues with poor cleaning effect by the first cleaning agent, thereby improving the cleaning effect of the first filter 1131, further prolonging the service life of the first filter 1131, and reducing the frequency of the user replacing the first filter 1131.

[0116] In some embodiments, the first switching member 130 is a rotary valve, which has two communication states. The first communication state is that the sample supply assembly 110 communicates with the liquid path preparation channel, the liquid-phase fluid supply assembly 120 and the chromatography column 150; the second communication state is that the liquid-phase fluid supply assembly 120, the sample liquid preparation channel 140 and the chromatography column 150 communicate.

[0117] In some embodiments, the liquid-phase fluid supply assembly 120 includes a first driving device 121 and a second driving device 122. The first driving device 121 is used to drive the first liquid-phase fluid to flow through the first switching member 130 and the chromatography column 150 in sequence. The second driving device 122 is used to drive the second liquid-phase fluid to flow through the first switching member 130 and the chromatography column 150 in sequence. The first driving device 121 and / or the second driving device 122 are also used to drive the sample liquid in the sample liquid preparation channel 140 to be transported to the chromatography column 150 through the liquid-phase fluid. The liquid-phase fluid needs to be driven by a relatively high pressure to flow through the chromatography column 150. The first driving device 121 can independently drive the first liquid-phase fluid to flow through the first switching member 130 and the chromatography column 150 in sequence. The second driving device 122 can independently drive the second liquid-phase fluid to flow through the first switching member 130 and the chromatography column 150 in sequence. That is, both the first driving device 121 and the second driving device 122 are high-pressure liquid path components. The sample supply assembly 110 is a low-pressure component, and the pressure output by the sample supply assembly 110 cannot drive the sample liquid to flow through the chromatography column 150. The first driving device 121 and / or the second driving device 122 are needed to drive the sample liquid to flow through the chromatography column 150 through the liquid-phase fluid. In this embodiment, the first driving device 121 is used to drive the first liquid-phase fluid to flow through the first switching member 130 and the chromatography column 150 in sequence, and the second driving device 122 is used to drive the second liquid-phase fluid to flow through the first switching member 130 and the chromatography column 150 in sequence. In specific applications, by adjusting the driving parameters of the first driving device 121 and the second driving device 122, a liquid-phase fluid containing the second liquid-phase fluid with different concentrations can be prepared online, thereby meeting the design requirements for gradient elution of the sample liquid.

[0118] As an embodiment, the first driving device 121 is used to suck the first liquid-phase fluid from the first container 300 and push it to the first switching member 130 and the chromatography column 150. The second driving device 122 is used to suck the second liquid-phase fluid from the second container 400 and push it to the first switching member 130 and the chromatography column 150.

[0119] As an implementation, the liquid-phase fluid supply assembly 120 further includes a mixing device 123. The mixing device 123 is provided with a first input port, a second input port, and an output port. The first driving device 121 is connected to the first input port, and the first switching member 130 is connected to the output port. The first liquid-phase fluid and the second liquid-phase fluid can flow into the mixing device 123 in proportion and be mixed to form a liquid-phase fluid (i.e., an eluent with different concentrations) containing the second liquid-phase fluid with different concentrations, and then enter the chromatography column 150 through the first switching member 130. In this implementation, eluents containing the second liquid-phase fluid with different concentrations can be prepared by adjusting the different mixing ratios of the first liquid-phase fluid and the second liquid-phase fluid, realizing the on-line preparation of eluents with different concentrations, meeting the requirements of high-pressure gradient elution, and facilitating the reduction of the amount of materials and material costs. The concentration of the eluent is specifically the volume ratio of the second liquid-phase fluid in the eluent, that is, the ratio of the volume of the second liquid-phase fluid to the total volume of the eluent (the total volume of the eluent is the sum of the volume of the second liquid-phase fluid and the volume of the first liquid-phase fluid).

[0120] As an implementation, the mixing device 123 is a three-way joint.

[0121] As an implementation, the ion concentration of the first liquid-phase fluid is less than the ion concentration of the second liquid-phase fluid.

[0122] As an implementation, the ionic strength of the first liquid-phase fluid is less than the ionic strength of the second liquid-phase fluid. Ionic strength is a measure of the ion concentration in a solution and is a function of the ion concentrations of all ions in the solution. When an ionic compound dissolves in a solvent, it dissociates into ions. The concentration of electrolytes in the solution affects the solubility of other salts, and the degree of influence is called the ionic strength. The elution ability of the first liquid-phase fluid is weaker than that of the second liquid-phase fluid. The first liquid-phase fluid can specifically be solution A, and the second liquid-phase fluid can specifically be solution B.

[0123] In some implementations, both the first driving device 121 and the second driving device 122 include piston pumps, and the operating pressure ranges of the first driving device 121 and the second driving device 122 are both from 0.5 MPa to 10 MPa.

[0124] In some implementations, the sample liquid preparation channel 140 is respectively connected to the sample supply assembly 110 and the liquid-phase fluid supply assembly 120 in different switching states of the first switching member 130. In the chromatographic analysis mode, when the first switching member 130 is in the first connection state, the sample liquid enters the sample preparation pipeline and waits. When the first switching member 130 switches to the second connection state, the sample preparation pipeline is respectively connected to the liquid-phase fluid supply assembly 120 and the chromatography column 150. In this way, under the drive of the liquid-phase fluid supply assembly 120, the sample liquid is transported to the chromatography column 150 for elution.

[0125] In some embodiments, the pH value of the first cleaning reagent is less than that of the second cleaning reagent. The pH value of the second cleaning agent is higher than that of the first cleaning agent, so that the cleaning effect of the second cleaning agent is better. On the basis of the cleaning method in the chromatographic mode, a second cleaning agent with a higher pH value, that is, a better cleaning effect, is added to supplement the cleaning, thereby improving the cleaning effect on the first filter 1131. Therefore, the service life of the first filter 1131 can be improved, and the frequency of the user replacing the first filter 1131 can be reduced.

[0126] In some embodiments, the second cleaning reagent is an alkaline reagent or a protease reagent. When the second cleaning agent is an alkaline reagent or a protease reagent, the cleaning effect on the first filter 1131 can be improved. On the basis of the cleaning method in the chromatographic mode, a second cleaning agent with a better cleaning effect is added to supplement the cleaning, thereby improving the cleaning effect on the first filter 1131. Therefore, the service life of the first filter 1131 can be improved, and the frequency of the user replacing the first filter 1131 can be reduced.

[0127] In some embodiments, the second cleaning reagent is an alkaline reagent, and the pH value of the second cleaning reagent is greater than or equal to 9 and less than or equal to 14. On the basis of cleaning the first filter 1131 in the chromatographic mode, a second cleaning reagent is added to perform cleaning in the first maintenance mode, improving the cleaning effect on the first filter 1131, and thus improving the service life of the first filter 1131.

[0128] In some embodiments, the first cleaning reagent and the reaction reagent are the same reagent.

[0129] In some embodiments, the reaction reagent and the first cleaning reagent share a liquid supply container (i.e., Figure 2 the reaction reagent container 500 in). Before the sample and the reagent react in the reaction container 111, the first suction and discharge driving device 1132 supplies the reaction reagent in the reaction reagent container 500 to the reaction container 111 along the second direction through the first filter 1131; after the sample liquid formed by the reagent and the sample is transported to the sample liquid preparation channel 140, the first suction and discharge driving device 1132 supplies the first cleaning reagent to the reaction container 111 along the second direction through the first filter 1131 to flush the first filter 1131. In summary, the first cleaning reagent and the reaction reagent share the same reagent, but the functions of this reagent are different at different stages.

[0130] In some embodiments, the reaction vessel 111 is connected to a waste liquid tank. After the sample liquid formed by the reaction reagent and the sample is transported to the sample liquid preparation channel 140, the first cleaning reagent provided by the reaction reagent container 500 is transported to the reaction vessel 111 along the second direction through the first filter 1131 under the drive of the first suction and discharge driving device 1132, and then discharged into the waste liquid tank.

[0131] In some embodiments, the sample is a blood sample and the reaction reagent is a hemolytic agent containing a surfactant. The blood sample contains various particles. The hemolytic agent with surfactant can dissolve the particles in the blood sample into smaller ones, and there are fewer particle fragments remaining on the first filter 1131 when flowing through the first filter 1131, thereby reducing the risk of blockage and facilitating ensuring the service life of the first filter 1131.

[0132] In some embodiments, the first cleaning reagent and the reaction reagent are the same reagent, the sample is a blood sample, and the reaction reagent is a hemolytic agent containing a surfactant. In this embodiment, using the hemolytic agent as the first cleaning reagent can further dissolve the red blood cell fragments accumulated on the first filter when cleaning the first filter, reducing the risk of blockage of the first filter 1131.

[0133] In some embodiments, the sample is a blood sample, the reaction reagent is a hemolytic agent, and the hemolytic agent is used to dissolve the red blood cells in the blood sample into red blood cell fragments with a length less than or equal to 1 μm. By dissolving the red blood cells into smaller fragments, it is beneficial to reduce the residue of the fragments on the first filter 1131 when flowing through the first filter 1131, reduce the risk of blockage of the first filter 1131, and is beneficial to improving the service life of the first filter 1131.

[0134] In some embodiments, in the reaction liquid obtained by the reaction of the sample and the reaction reagent, the proportion of the number of particles with a size of 0 μm to 1 μm in the total number of particles is 40% to 90%, and the proportion of the number of particles with a size of 1 μm to 2 μm in the total number of particles is 10% to 60%. By controlling the proportion of particles within a specific length range, it is beneficial to reduce the residue of the fragments on the first filter 1131 when flowing through the first filter 1131, reduce the risk of blockage of the first filter 1131, and is beneficial to improving the service life of the first filter 1131.

[0135] In some embodiments, the sample is a blood sample, the reaction reagent is a hemolytic agent, and the hemolytic agent is used to dissolve the red blood cells in the blood sample into red blood cell fragments with a length less than or equal to 1 μm; and in the reaction liquid obtained by the reaction of the sample and the reaction reagent, the proportion of the number of particles with a size of 0 μm to 1 μm in the total number of particles is 40% to 90%, and the proportion of the number of particles with a size of 1 μm to 2 μm in the total number of particles is 10% to 60%.

[0136] In some embodiments, the liquid chromatography analyzer 100 pre-stores at least one of the following first preset conditions: reaching a first preset duration since the last execution of the first maintenance mode; the cumulative number of times the liquid chromatography analyzer has executed chromatographic analysis projects reaching a first preset number since the last execution of the first maintenance mode; reaching a first preset time point of a first preset cycle; obtaining information that the first filter 1131 needs to be cleaned and maintained according to an operation instruction received by the human-machine interaction device.

[0137] The first controller is further configured to: when any one of the first preset conditions is met, execute the first maintenance mode or output information prompting the execution of the first maintenance mode. By presetting conditions such as the number of uses and the usage duration, automatic maintenance is performed or a maintenance prompt message is output after the conditions are met to prompt the user that the sample analyzer needs to execute the first maintenance mode to clean the first filter 1131, thereby extending the service life of the first filter 1131.

[0138] In some embodiments, the sample dispensing device 112 includes a sample needle, which is used to aspirate a sample from a sample container located at the aspiration level and dispense at least a part of the aspirated sample into the reaction container 111, and is also used to aspirate a second cleaning reagent from a cleaning reagent container located at the aspiration level and dispense the aspirated second cleaning reagent into the reaction container 111.

[0139] In the first maintenance mode, the first controller is further configured to: before controlling the first liquid path assembly 113 to drive the second cleaning reagent to flow through the first filter 1131 to clean the first filter 1131, control the sample dispensing device 112 to aspirate the second cleaning reagent from a cleaning reagent container located at the aspiration level and dispense it into the reaction container 111.

[0140] The above control of the first liquid path assembly 113 to drive the second cleaning reagent to flow through the first filter 1131 includes: controlling the first liquid path assembly 113 to drive the second cleaning reagent to flow through the first filter 1131 from the reaction container 111 in the first direction. Cleaning the first filter 1131 by aspirating the second cleaning liquid with a reagent needle improves the cleaning effect on the first filter 1131 compared to cleaning the first filter 1131 in the chromatographic analysis mode, so as to extend the service life of the first filter 1131.

[0141] In some embodiments, the liquid chromatography analyzer 100 further includes a cleaning reagent placement assembly and a first transfer assembly. The cleaning reagent placement assembly is used for placing a cleaning reagent container loaded with the second cleaning reagent, and the first transfer assembly is used to transfer the cleaning reagent container loaded with the second cleaning reagent from the cleaning reagent placement assembly to the aspiration level.

[0142] In the first maintenance mode, the first controller is further configured to: before controlling the sample dispensing device 112 to aspirate a second cleaning reagent from the cleaning reagent container located at the aspiration level and dispense it into the reaction container 111, control the first transfer assembly to transfer the cleaning reagent container loaded with the second cleaning reagent from the cleaning reagent loading assembly to the aspiration level. By providing the cleaning reagent loading assembly and the first transfer assembly, after reaching the preset number of times or duration, the liquid chromatography analyzer automatically executes the first maintenance mode, reducing the workload of the user.

[0143] In some embodiments, the first liquid path assembly 113 further includes a first suction and discharge driving device 1132, a second switching member 1133, a third switching member 1134, and a liquid suction pipeline 1135. The liquid suction pipeline 1135 is used to aspirate the reaction reagent from the reaction reagent container 500 loaded with the reaction reagent. The first suction and discharge driving device 1132 is switchably connected to the liquid suction pipeline 1135 and the third switching member 1134 through the second switching member 1133. The second switching member 1133 is switchably connected to the reaction container 111 and the first switching member 130 through the third switching member 1134. The first filter 1131 is provided between the reaction container 111 and the third switching member 1134.

[0144] In some embodiments, both the second switching member 1133 and the third switching member 1134 are three-way valves. The second switching member 1133 is used to connect the first suction and discharge driving device 1132 to one of the reaction reagent container 500 and the third switching member 1134. That is, the second switching member 1133 has two states. State one is that the first suction and discharge driving device 1132 is connected to the reaction reagent container 500, and the first suction and discharge driving device 1132 and the third switching member 1134 are disconnected. State two is that the first suction and discharge driving device 1132 is disconnected from the reaction reagent container 500, and the first suction and discharge driving device 1132 and the third switching member 1134 are connected. Similarly, the third switching member 1134 is used to connect the second switching member 1133 to one of the fourth switching member 180 and the reaction container 111. That is, the third switching member 1134 has two states. State one is that the second switching member 1133 and the fourth switching member 180 are connected, and the second switching member 1133 is disconnected from the reaction container 111. State two is that the second switching member 1133 and the fourth switching member 180 are disconnected, and the second switching member 1133 is connected to the reaction container 111.

[0145] In some embodiments, the liquid chromatography analyzer 100 further includes a waste liquid channel 190. In the first communication state, the first switching member 130 communicates with the sample supply assembly 110, the sample liquid preparation channel 140, and the waste liquid channel 190, and also communicates with the liquid phase fluid supply assembly 120 and the chromatography column 150. In the second communication state, the first switching member 130 communicates with the liquid phase fluid supply assembly 120, the sample liquid preparation channel 140, and the chromatography column 150, and also communicates with the sample supply assembly 110 and the waste liquid channel 190. Controlling the first liquid path assembly 113 to drive the second cleaning reagent to flow through the first filter 1131 includes: controlling the first liquid path assembly 113 to drive the second cleaning reagent to flow through the first filter 1131 in the first direction to clean the first filter 1131 and conveying it to the waste liquid channel 190 through the first switching member 130.

[0146] In some embodiments, the first switching member 130 is a six-way rotary valve. In the first communication state of the first switching member 130, the first liquid path assembly 113, the sample liquid preparation channel 140, and the waste liquid channel 190 are in communication, and the liquid phase fluid supply assembly 120 and the chromatography column 150 are in communication. In the second communication state, the first liquid path assembly 113 and the waste liquid channel 190 are in communication, and the liquid phase fluid supply assembly 120, the sample liquid preparation channel 140, and the chromatography column 150 are in communication.

[0147] In some embodiments, the first suction and discharge driving device 1132 is an injection pump. The working pressure of the first suction and discharge driving device 1132 is less than 200 KPa.

[0148] In some embodiments, the liquid chromatography analyzer 100 further includes a second filter 170. The second filter 170 is disposed between the first switching member 130 and the chromatography column 150. The second filter 170 is used to further protect the chromatography column 150 to improve the service life of the chromatography column 150. Since the first filter 1131 has already filtered the sample liquid once before the second filter 170, foreign matters with larger particle sizes such as white blood cells, test tube debris, hair, and some hemolysis fragments can be intercepted in advance, thereby reducing the filtration load of the second filter 170, and further facilitating ensuring the life of the second filter 170 and greatly reducing the replacement frequency of the second filter 170.

[0149] The filtration accuracy of the second filter 170 is greater than that of the first filter 1131. In the chromatographic mode, the sample liquid passes through the first filter 1131 and the second filter 170 in sequence. Since the filtration accuracy of the second filter 170 is greater than that of the first filter 1131, the sample liquid is roughly filtered by the first filter 1131, and foreign matters with larger particle sizes such as white blood cells, test tube debris, hair, and some hemolysis fragments can be intercepted in advance, thereby reducing the filtration load of the second filter 170 and further extending the life of the second filter 170.

[0150] In some embodiments, the second filter 170 includes a first filter layer and a second filter layer. The first filter layer is disposed between the first switching member 130 and the second filter layer. The aperture diameter of the filter holes of the first filter layer is in the range of 0.5 μm to 20 μm, and the aperture diameter of the filter holes of the second filter layer is in the range of 0.2 μm to 5 μm. The sample liquid flows through the first filter layer and the second filter layer in sequence. The filtration accuracy of the second filter layer is greater than that of the first filter layer. The sample liquid completes coarse filtration in the first filter layer, and foreign matters with larger particle sizes can be intercepted in advance, thereby reducing the filtration load of the second filter layer and further extending the service life of the second filter layer, so as to improve the service life of the entire second filter 170.

[0151] In some embodiments, the aperture diameter of the filter holes of the first filter 1131 is in the range of 10 μm to 200 μm.

[0152] In some embodiments, the liquid chromatography analyzer 100 pre-stores preset replacement conditions, and the first controller is further configured to: when the chromatography column 150 meets the preset replacement conditions, output a warning signal to prompt to replace the chromatography column 150 and the second filter 170 simultaneously. Since the service life of the second filter 170 is extended, the second filter 170 with a short service life in the past can be replaced synchronously with the chromatography column 150 with a longer service life. By setting preset conditions to remind the user to replace the chromatography column 150 and the second filter 170, the operation frequency of the user is reduced.

[0153] In some embodiments, the liquid chromatography analyzer 100 further includes a fourth switching member 180. The second filter 170 is switchably connected to the chromatography column 150 and the waste liquid channel 190 through the fourth switching member 180;

[0154] The first controller is further configured with a second maintenance mode;

[0155] In the second maintenance mode, the first controller is configured to perform the following actions: when the first switching member 130 is in the first communication state, control the first liquid path assembly 113 to drive the second cleaning reagent to be delivered to the sample liquid preparation channel 140 through the first switching member 130, control the first switching member 130 to switch to the second communication state, and control the second liquid path assembly to drive the second cleaning reagent in the sample liquid preparation channel 140 to flow through the second filter 170, the fourth switching member 180, and the waste liquid channel 190 in sequence, so as to clean the second filter 170 with the second cleaning reagent;

[0156] In some embodiments, the fourth switching member 180 is a rotary valve or a three-way valve. The fourth switching member 180 has two states. State one is that the first switching member 130 is in communication with the chromatography column 150, and the first switching member 130 is disconnected from the waste liquid channel 190. State two is that the first switching member 130 is disconnected from the chromatography column 150, and the first switching member 130 is in communication with the waste liquid channel 190.

[0157] In some embodiments, the liquid chromatography analyzer 100 pre-stores at least one of the following second preset conditions: reaching a second preset duration since the last execution of the second maintenance mode; the number of times the liquid chromatography analyzer has performed chromatography analysis projects cumulatively since the last execution of the second maintenance mode reaching a second preset number; reaching a second preset time point of a second preset cycle; obtaining information that the second filter 170 needs to be cleaned and maintained according to an operation instruction received by the man-machine interaction device. The first controller is further configured to: when any one of the second preset conditions is satisfied, execute the second maintenance mode or output information prompting the execution of the second maintenance mode.

[0158] Embodiment 2;

[0159] The liquid chromatography analyzer 100 provided in this embodiment is mainly different from that in Embodiment 1 in terms of the operations performed in the chromatography analysis mode and the types of the second cleaning agents, specifically as follows: in Embodiment 1, the cleaning and maintenance of the first filter 1131 include cleaning in the chromatography analysis mode and cleaning in the first maintenance mode; while in this embodiment, the cleaning and maintenance of the first filter 1131 include cleaning the first filter 1131 with an alkaline reagent or a protease reagent in the first maintenance mode.

[0160] Specifically, in this embodiment, the liquid chromatography analyzer 100 includes a sample supply assembly 110, a liquid phase fluid supply assembly 120, a first switching member 130, a sample solution preparation channel 140, a chromatography column 150, a detector 160, and a first controller. The first switching member 130 has a switchable first communication state and a second communication state. In the first communication state, the first switching member 130 is in communication with the sample supply assembly 110, the sample solution preparation channel 140, and is in communication with the liquid phase fluid supply assembly 120 and the chromatography column 150. In the second communication state, the first switching member 130 is in communication with the liquid phase fluid supply assembly 120, the sample solution preparation channel 140, and the chromatography column 150. The chromatography column 150 is disposed between the first switching member 130 and the detector 160.

[0161] The sample supply component 110 includes a reaction container 111, a sample dispensing device 112 and a first liquid circuit component 113. The reaction container 111 is used to provide a reaction field for the sample and the reaction reagent to form a sample liquid. The sample dispensing device 112 is used to absorb the sample from the sample container and dispense at least part of the absorbed sample into the reaction container 111. The first liquid circuit component 113 is used to drive the sample liquid in the reaction container 111 to be transported to the sample liquid preparation channel 140 through the first switching component 130 when the first switching component 130 is in a first connected state. The first liquid circuit component 113 includes a first filter 1131. The first filter 1131 is arranged between the reaction container 111 and the first switching component 130 to filter the sample liquid transported from the reaction container 111 to the sample liquid preparation channel 140. The first liquid circuit component 113 is also used to drive the second cleaning reagent to clean the first filter 1131.

[0162] The liquid-phase fluid supply component 120 is used to: when the first switching component 130 is in the second connected state, drive the sample liquid in the sample liquid preparation channel 140 to be transported to the chromatography column 150 through the liquid-phase fluid; and when the first switching component 130 is in the second connected state, drive the liquid-phase fluid to flow through the first switching component 130 and the chromatography column 150 in sequence.

[0163] The chromatography column 150 is used to adsorb the sample liquid and to provide a liquid phase fluid to elute the sample liquid to form a test liquid.

[0164] The detector 160 is used to perform chromatographic analysis on the test liquid flowing out of the chromatography column 150 .

[0165] The first controller is configured with a chromatography mode and a first maintenance mode.

[0166] In the chromatography analysis mode, the first controller is configured to perform the following actions: when the first switching member 130 is in the first connected state, control the first liquid circuit component 113 to drive the sample liquid from the reaction container 111 to flow through the first filter 1131 and be transported to the sample liquid preparation channel 140 through the first switching member 130, control the first switching member 130 to switch to the second connected state, control the second liquid circuit component to drive the sample liquid in the sample liquid preparation channel 140 to be transported to the chromatography column 150 through the liquid phase fluid, control the first switching member 130 to switch to the first connected state, control the second liquid circuit component to drive the liquid phase fluid to be transported to the chromatography column 150 through the first switching member 130 to elute the sample liquid, and control the detector 160 to perform chromatographic analysis on the test liquid flowing out of the chromatography column 150.

[0167] In the first maintenance mode, the first controller is configured to perform the following actions: control the first fluid circuit component 113 to drive the second cleaning reagent to flow through the first filter 1131 to clean the first filter 1131 .

[0168] Among them, the second cleaning reagent is an alkaline reagent or a protease reagent. Using an alkaline reagent or a protease reagent with better cleaning effect to clean and maintain the first filter 1131 improves the service life of the first filter 1131.

[0169] In some embodiments, the sample dispensing device 112 includes a sample needle. The sample needle is used to aspirate a sample from a sample container located at the aspiration level and dispense at least a portion of the aspirated sample into the reaction container 111, and is also used to aspirate the second cleaning reagent from a cleaning reagent container located at the aspiration level and dispense the aspirated second cleaning reagent into the reaction container 111.

[0170] In the first maintenance mode, the first controller is further configured to: before controlling the first liquid path component 113 to drive the second cleaning reagent to flow through the first filter 1131 to clean the first filter 1131, control the sample dispensing device 112 to aspirate the second cleaning reagent from a cleaning reagent container located at the aspiration level and dispense it into the reaction container 111.

[0171] Controlling the first liquid path component 113 to drive the second cleaning reagent to flow through the first filter 1131 includes: controlling the first liquid path component 113 to drive the second cleaning reagent to flow through the first filter 1131 from the reaction container 111 in the first direction.

[0172] In some embodiments, the liquid chromatography analyzer 100 further includes a cleaning reagent placement component and a first transmission component. The cleaning reagent placement component is used to place a cleaning reagent container loaded with the second cleaning reagent, and the first transmission component is used to transmit the cleaning reagent container loaded with the second cleaning reagent from the cleaning reagent placement component to the aspiration level; in the first maintenance mode, the first controller is further configured to: before controlling the sample dispensing device 112 to aspirate the second cleaning reagent from a cleaning reagent container located at the aspiration level and dispense it into the reaction container 111, control the first transmission component to transmit the cleaning reagent container loaded with the second cleaning reagent from the cleaning reagent placement component to the aspiration level.

[0173] Except for the above, other parts of the liquid chromatography analyzer 100 provided in this embodiment can refer to Embodiment 1 and will not be elaborated here.

[0174] Embodiment 3:

[0175] The liquid chromatograph analyzer 100 provided in this embodiment is different from that in the first embodiment mainly in that the objects maintained by the cleaning liquid are different, which is specifically embodied in that: in the first embodiment, the first filter 1131 is cleaned together with a first cleaning liquid and a second cleaning liquid with two different components to increase the service life of the first filter 1131; in the second embodiment, a hemolytic agent containing a surfactant with stronger hemolytic ability is used, which can dissolve the blood components in the sample into smaller fragments, thereby reducing the filtering load on the second filter 170 when flowing through the second filter 170, thereby increasing the service life of the second filter 170.

[0176] Specifically, in this embodiment, the liquid chromatograph analyzer 100 includes a sample supply component 110, a liquid phase fluid supply component 120, a first switching member 130, a sample liquid preparation channel 140, a second filter 170, a chromatography column 150, a detector 160 and a first controller. The first switching member 130 has a switchable first connection state and a second connection state. In the first connection state, the first switching member 130 connects the sample supply component 110, the sample liquid preparation channel 140 and the liquid phase fluid supply component 120 and the second filter 170. In the second connection state, the first switching member 130 connects the liquid phase fluid supply component 120, the sample liquid preparation channel 140 and the second filter 170, and the chromatography column 150 is disposed between the second filter 170 and the detector 160.

[0177] The sample supply assembly 110 includes a reaction container 111, a sample dispensing device 112 and a first liquid circuit assembly 113. The reaction container 111 is used to provide a reaction site for the sample and the reaction reagent to form a sample liquid. The sample dispensing device 112 is used to absorb the sample from the sample container and dispense at least part of the absorbed sample into the reaction container 111. The first liquid circuit assembly 113 distributes the reaction reagent to the reaction container 111 and drives the sample liquid in the reaction container 111 to be transported to the sample liquid preparation channel 140 through the first switching member 130 when the first switching member 130 is in a first connected state.

[0178] The liquid-phase fluid supply component 120 is used to: when the first switching component 130 is in the second connected state, drive the sample liquid in the sample liquid preparation channel 140 to be transported to the chromatography column 150 through the second filter 170 by the liquid-phase fluid; and when the first switching component 130 is in the first connected state, drive the liquid-phase fluid to flow through the first switching component 130, the second filter 170 and the chromatography column 150 in sequence.

[0179] The chromatography column 150 is used to adsorb the sample liquid and to provide a liquid phase fluid to elute the sample liquid to form a test liquid.

[0180] The detector 160 is used to perform chromatographic analysis on the test liquid flowing out of the chromatography column 150 .

[0181] The first controller is configured with a chromatographic analysis mode.

[0182] In the chromatographic analysis mode, the first controller is configured to perform the following actions: when the first switching member 130 is in the first communication state, control the first liquid path assembly 113 to dispense a reaction reagent to the reaction vessel 111, control the sample dispensing device 112 to dispense a sample to the reaction vessel 111, control the first switching member 130 to switch to the second communication state, control the second liquid path assembly to drive the sample liquid in the sample liquid preparation channel 140 through the liquid phase fluid to be delivered to the chromatography column 150 via the second filter 170, control the first switching member 130 to switch to the first communication state, control the second liquid path assembly to drive the liquid phase fluid to be delivered to the chromatography column 150 via the first switching member 130 and the second filter 170 to elute the sample liquid, and control the detector 160 to perform chromatographic analysis on the liquid to be measured flowing out of the chromatography column 150.

[0183] Wherein, the reaction reagent is a hemolytic agent containing a surfactant.

[0184] Except for the above, for other parts of the liquid chromatography analyzer 100 provided in this embodiment, reference can be made to Embodiments 1 to 2, which will not be elaborated here.

[0185] Embodiment 4:

[0186] The main difference between the liquid chromatography analyzer 100 provided in this embodiment and Embodiment 1 lies in the different objects of cleaning liquid maintenance, specifically reflected in: in Embodiment 1, the first filter 1131 is jointly cleaned by the first cleaning liquid and the second cleaning liquid with different components to improve the service life of the first filter 1131; in Embodiment 2, by increasing the proportion of fine fragments in the reaction liquid, the filtration load on the second filter 170 when the reaction liquid flows through the second filter 170 is reduced, thereby improving the service life of the second filter 170.

[0187] Specifically, in this embodiment, the liquid chromatography analyzer 100 includes a sample supply assembly 110, a liquid phase fluid supply assembly 120, a first switching member 130, a sample liquid preparation channel 140, a second filter 170, a chromatography column 150, a detector 160, and a first controller. The first switching member 130 has a switchable first communication state and a second communication state. In the first communication state, the first switching member 130 connects the sample supply assembly 110, the sample liquid preparation channel 140, and connects the liquid phase fluid supply assembly 120 and the second filter 170. In the second communication state, the first switching member 130 connects the liquid phase fluid supply assembly 120, the sample liquid preparation channel 140, and the second filter 170. The chromatography column 150 is disposed between the second filter 170 and the detector 160.

[0188] The sample supply component 110 includes a reaction container 111, a sample dispensing device 112 and a first liquid circuit component 113. The reaction container 111 is used to provide a reaction site for the sample and the reaction reagent to form a sample liquid. The sample dispensing device 112 is used to absorb the sample from the sample container and dispense at least part of the absorbed sample into the reaction container 111. The first liquid circuit component 113 distributes the reaction reagent to the reaction container 111 and is used to drive the sample liquid in the reaction container 111 to be transported to the sample liquid preparation channel 140 through the first switching component 130 when the first switching component 130 is in a first connected state.

[0189] The liquid-phase fluid supply component 120 is used to: when the first switching component 130 is in the second connected state, drive the sample liquid in the sample liquid preparation channel 140 to be transported to the chromatography column 150 through the second filter 170 by the liquid-phase fluid; and when the first switching component 130 is in the first connected state, drive the liquid-phase fluid to flow through the first switching component 130, the second filter 170 and the chromatography column 150 in sequence.

[0190] The chromatography column 150 is used to adsorb the sample liquid and to provide a liquid phase fluid to elute the sample liquid to form a test liquid.

[0191] The detector 160 is used to perform chromatographic analysis on the test liquid flowing out of the chromatography column 150 .

[0192] The first controller is configured with a chromatography mode.

[0193] In the chromatographic analysis mode, the first controller is configured to perform the following actions: when the first switching member 130 is in the first connected state, control the first liquid circuit component 113 to dispense reaction reagents to the reaction container 111, control the sample dispensing device 112 to dispense samples to the reaction container 111, control the first switching member 130 to switch to the second connected state, control the second liquid circuit component to drive the sample liquid in the sample liquid preparation channel 140 to be transported to the chromatography column 150 through the second filter 170 through the liquid phase fluid, control the first switching member 130 to switch to the first connected state, control the second liquid circuit component to drive the liquid phase fluid to be transported to the chromatography column 150 through the first switching member 130 and the second filter 170 to elute the sample liquid, and control the detector 160 to perform chromatographic analysis on the test liquid flowing out of the chromatography column 150.

[0194] Among them, in the reaction liquid obtained by the reaction of the reaction reagent and the sample, the number of particles with a size of 0um to 1um accounts for 40% to 90% of the total number of particles, and the number of particles with a size of 1um to 2um accounts for 10% to 60% of the total number of particles.

[0195] In addition to the above, for other parts of the liquid chromatography analyzer 100 provided in this embodiment, reference can be made to Embodiments 1 to 3, which will not be elaborated here.

[0196] Embodiment 5:

[0197] The main difference between the sample analysis system 200 provided in this embodiment and that in Embodiment 1 lies in the different usage scenarios, specifically reflected in: in Embodiment 1, the first filter 1131 of the single liquid chromatography analyzer is cleaned; in this embodiment, the sample analysis system 200 is composed of multiple components including the liquid chromatography analyzer 100, the second transmission component 220, etc.

[0198] Specifically, in this embodiment, as Figure 5 shown, the sample analysis system 200 includes a sample input device 210, and the sample input device 210 is at least used for placing a sample container loaded with a sample to achieve sample loading.

[0199] A liquid chromatography analyzer, and the liquid chromatography analyzer 100 is used to aspirate a sample from the sample container and perform chromatographic analysis on at least part of the aspirated sample.

[0200] A second transmission component 220, and the second transmission track is used to transmit the sample container from the sample input device 210 to the liquid chromatography analyzer.

[0201] A second controller, and the second controller is used to control the second transmission component 220 to transmit the sample container to the liquid chromatography analyzer 100.

[0202] Among them, the liquid chromatography analyzer 100 includes a sample supply component 110, a liquid phase fluid supply component 120, a first switching member 130, a sample liquid preparation channel 140, a chromatography column 150, a detector 160, and a first controller. The first switching member 130 has a switchable first communication state and a second communication state. In the first communication state, the first switching member 130 connects the sample supply component 110, the sample liquid preparation channel 140, and connects the liquid phase fluid supply component 120 and the chromatography column 150. In the second communication state, the first switching member 130 connects the liquid phase fluid supply component 120, the sample liquid preparation channel 140, and the chromatography column 150. The chromatography column 150 is arranged between the first switching member 130 and the detector 160.

[0203] The sample supply component 110 includes a reaction container 111, a sample dispensing device 112 and a first liquid circuit component 113. The reaction container 111 is used to provide a reaction field for the sample and the reaction reagent to form a sample liquid. The sample dispensing device 112 is used to absorb the sample from the sample container and dispense at least part of the absorbed sample into the reaction container 111. The first liquid circuit component 113 is used to drive the sample liquid in the reaction container 111 to be transported to the sample liquid preparation channel 140 through the first switching component 130 when the first switching component 130 is in a first connected state. The first liquid circuit component 113 includes a first filter 1131. The first filter 1131 is arranged between the reaction container 111 and the first switching component 130 to filter the sample liquid transported from the reaction container 111 to the sample liquid preparation channel 140. The first liquid circuit component 113 is also used to drive the first cleaning reagent to clean the first filter 1131, and to drive the second cleaning reagent to clean the first filter 1131.

[0204] The liquid-phase fluid supply component 120 is used to: when the first switching component 130 is in the second connected state, drive the sample liquid in the sample liquid preparation channel 140 to be transported to the chromatography column 150 through the liquid-phase fluid; and when the first switching component 130 is in the first connected state, drive the liquid-phase fluid to flow through the first switching component 130 and the chromatography column 150 in sequence.

[0205] The chromatography column 150 is used to adsorb the sample liquid and to provide a liquid phase fluid to elute the sample liquid to form a test liquid.

[0206] The detector 160 is used to perform chromatographic analysis on the test liquid flowing out of the chromatography column 150 .

[0207] The first controller is configured with a chromatography mode and a first maintenance mode.

[0208] In the chromatography analysis mode, the first controller is configured to perform the following actions: when the first switching member 130 is in a first connected state, the first liquid circuit component 113 is controlled to drive the sample liquid from the reaction container 111 to flow through the first filter 1131 along a first direction and be transported to the sample liquid preparation channel 140 through the first switching member 130; after the sample liquid is transported to the sample liquid preparation channel 140, the first liquid circuit component 113 is controlled to drive the first cleaning reagent to flow through the first filter 1131 along a second direction to reversely flush the first filter 1131 with the first cleaning reagent, wherein the first direction and the second direction are two opposite directions.

[0209] In the first maintenance mode, the first controller is configured to perform the following actions: control the first fluid circuit component 113 to drive the second cleaning reagent to flow through the first filter 1131 to clean the first filter 1131 .

[0210] The first cleaning reagent and the second cleaning reagent are two reagents with different components.

[0211] In some embodiments, the sample dispensing device 112 includes a sample needle, which is used to aspirate a sample from a sample container located at the aspiration level and dispense at least a portion of the aspirated sample into the reaction container 111, and is also used to aspirate the second cleaning reagent from a cleaning reagent container located at the aspiration level and dispense the aspirated second cleaning reagent into the reaction container 111.

[0212] In the first maintenance mode, the first controller is further configured to: before controlling the first liquid path component 113 to drive the second cleaning reagent to flow through the first filter 1131 to clean the first filter 1131, control the sample dispensing device 112 to aspirate the second cleaning reagent from a cleaning reagent container located at the aspiration level and dispense it into the reaction container 111.

[0213] Controlling the first liquid path component 113 to drive the second cleaning reagent to flow through the first filter 1131 includes: controlling the first liquid path component 113 to drive the second cleaning reagent to flow through the first filter 1131 from the reaction container 111 in the first direction.

[0214] Preferably, the sample analysis system 200 further includes a cleaning reagent loading component for placing a cleaning reagent container loaded with the second cleaning reagent, and the second transmission component 220 is further used to transmit the cleaning reagent container loaded with the second cleaning reagent from the cleaning reagent loading component to the liquid chromatography analyzer; in the first maintenance mode, the first controller is further configured to: before controlling the sample dispensing device 112 to aspirate the second cleaning reagent from a cleaning reagent container located at the aspiration level and dispense it into the reaction container 111, control the second transmission component 220 to transmit the cleaning reagent container loaded with the second cleaning reagent from the cleaning reagent loading component to the liquid chromatography analyzer.

[0215] This embodiment also provides a computer-readable storage medium storing a computer program, which when executed by a processor (such as the above-mentioned first controller), enables the processor to implement the steps of the control method of the above-mentioned liquid chromatography analyzer or sample analysis system. Among them, the computer-readable storage medium can be an internal storage unit of the above-mentioned liquid chromatography analyzer or sample analysis system, such as the hard disk or memory of the liquid chromatography analyzer or sample analysis system; or, the computer-readable storage medium can also be an external storage device of the liquid chromatography analyzer or sample analysis system, such as a plug-in hard disk equipped on the liquid chromatography analyzer or sample analysis system, a SmartMedia Card (SMC), a Secure Digital (SD) card, a Flash Card, etc.

[0216] In addition to the above, for other parts of the liquid chromatography analyzer 100 provided in this embodiment, reference can be made to Embodiments 1 to 4, which will not be elaborated here.

[0217] The above are only the preferred embodiments of the present invention, and do not limit the patent scope of the present invention accordingly. Any equivalent structural transformation made by using the content of the specification and drawings of the present invention under the inventive concept of the present invention, or direct / indirect application in other related technical fields is included in the patent protection scope of the present invention.

Claims

1. A liquid chromatography analyzer, characterized in that, It includes a sample supply component, a liquid-phase fluid supply component, a first switching member, a sample solution preparation channel, a chromatography column, a detector, and a first controller. The first switching member has a switchable first communication state and a second communication state. In the first communication state, the first switching member connects the sample supply component, the sample solution preparation channel, and also connects the liquid-phase fluid supply component and the chromatography column. In the second communication state, the first switching member connects the liquid-phase fluid supply component, the sample solution preparation channel, and the chromatography column. The chromatography column is provided between the first switching member and the detector; The sample supply component includes a reaction container, a sample dispensing device, and a first liquid path component. The reaction container is used to provide a reaction place for the sample and the reaction reagent to form a sample solution. The sample dispensing device is used to suck the sample from the sample container and dispense at least part of the sucked sample into the reaction container. The first liquid path component is used to drive the sample solution in the reaction container to be transported to the sample solution preparation channel through the first switching member when the first switching member is in the first communication state. The first liquid path component includes a first filter. The first filter is provided between the reaction container and the first switching member to filter the sample solution transported from the reaction container to the sample solution preparation channel. The first liquid path component is also used to drive a first cleaning reagent to clean the first filter, and to drive a second cleaning reagent to clean the first filter; The liquid-phase fluid supply component is used for: when the first switching member is in the second communication state, driving the sample solution in the sample solution preparation channel to be transported to the chromatography column by the liquid-phase fluid, and when the first switching member is in the first communication state, driving the liquid-phase fluid to flow through the first switching member and the chromatography column in sequence; The chromatography column is used to adsorb the sample solution, and to allow the liquid-phase fluid to elute the sample solution to form a test solution; The detector is used to perform chromatographic analysis on the test solution flowing out of the chromatography column; The first controller is configured with a chromatographic analysis mode and a first maintenance mode; In the chromatographic analysis mode, the first controller is configured to perform the following actions: when the first switching member is in the first communication state, control the first liquid path component to drive the sample solution to flow from the reaction container along a first direction through the first filter and be transported to the sample solution preparation channel through the first switching member. After the sample solution is transported to the sample solution preparation channel, control the first liquid path component to drive the first cleaning reagent to flow through the first filter along a second direction to backwash the first filter with the first cleaning reagent, where the first direction and the second direction are two opposite directions; In the first maintenance mode, the first controller is configured to perform the following actions: control the first liquid path component to drive the second cleaning reagent to flow through the first filter to clean the first filter; The first cleaning reagent and the second cleaning reagent are two reagents with different components.

2. The liquid chromatography analyzer according to claim 1, wherein The pH value of the first cleaning reagent is less than that of the second cleaning reagent.

3. The liquid chromatography analyzer according to claim 1, characterized in that, The second cleaning reagent is an alkaline reagent or a protease reagent.

4. The liquid chromatograph according to claim 3, characterized in that, The second cleaning reagent is an alkaline reagent, and the pH value of the second cleaning reagent is greater than or equal to 9 and less than or equal to 14.

5. The liquid chromatography analyzer according to claim 1, characterized in that, The first cleaning reagent and the reaction reagent are the same reagent; and / or, The sample is a blood sample, and the reaction reagent is a hemolytic agent containing a surfactant.

6. The liquid chromatography analyzer according to any one of claims 1 to 5, characterized in that, The sample is a blood sample, the reaction reagent is a hemolytic agent, and the hemolytic agent is used to dissolve red blood cells in the blood sample into red blood cell fragments with a length less than or equal to 1 μm; and / or, In the reaction solution obtained by reacting the sample with the reaction reagent, the proportion of the number of particles with a size of 0 μm to 1 μm in the total number of particles is 40% to 90%, and the proportion of the number of particles with a size of 1 μm to 2 μm in the total number of particles is 10% to 60%.

7. The liquid chromatography analyzer according to claim 1, characterized in that, The liquid chromatography analyzer pre-stores at least one of the following first preset conditions: reaching a first preset duration since the last execution of the first maintenance mode; The cumulative number of times the liquid chromatography analyzer has executed chromatographic analysis items since the last execution of the first maintenance mode reaches a first preset number; Reaching a first preset time point of a first preset cycle; obtaining information that the first filter needs to be cleaned and maintained according to an operation instruction received by the man-machine interaction device; The first controller is further configured to: when any one of the first preset conditions is satisfied, execute the first maintenance mode or output information prompting the execution of the first maintenance mode.

8. The liquid chromatography analyzer according to any one of claims 1 to 5 or claim 7, characterized in that The sample dispensing device includes a sample needle, and the sample needle is used to suck the sample from the sample container located at the suction level and dispense at least part of the sucked sample into the reaction container and to suck the second cleaning reagent from the cleaning reagent container located at the suction level and dispense the sucked second cleaning reagent into the reaction container; In the first maintenance mode, the first controller is further configured to: before controlling the first liquid path component to drive the second cleaning reagent to flow through the first filter to clean the first filter, control the sample dispensing device to suck the second cleaning reagent from the cleaning reagent container located at the suction level and dispense it into the reaction container; Controlling the first liquid path component to drive the second cleaning reagent to flow through the first filter includes: controlling the first liquid path component to drive the second cleaning reagent to flow through the first filter from the reaction container along the first direction.

9. The liquid chromatography analyzer according to claim 8, wherein, The liquid chromatography analyzer further includes a cleaning reagent placing component and a first transmission component. The cleaning reagent placing component is used for placing the cleaning reagent container loaded with the second cleaning reagent, and the first transmission component is used to transmit the cleaning reagent container loaded with the second cleaning reagent from the cleaning reagent placing component to the suction level; In the first maintenance mode, the first controller is further configured to: before controlling the sample dispensing device to aspirate the second cleaning reagent from the cleaning reagent container located at the aspiration level and dispense it into the reaction container, control the first transfer assembly to transfer the cleaning reagent container loaded with the second cleaning reagent from the cleaning reagent loading assembly to the aspiration level.

10. The liquid chromatography analyzer according to any one of claims 1 to 5 or claim 7, characterized in that, The first liquid path assembly further includes a first suction and discharge driving device, a second switching member, a third switching member, and a suction line for aspirating the reaction reagent from the reaction reagent container loaded with the reaction reagent. The first suction and discharge driving device is switchably connected to the suction line and the third switching member through the second switching member. The second switching member is switchably connected to the reaction container and the first switching member through the third switching member. The first filter is provided between the reaction container and the third switching member; and / or, The liquid chromatography analyzer further includes a waste liquid channel. In the first connection state, the first switching member connects the sample supply assembly, the sample liquid preparation channel, and the waste liquid channel, and connects the liquid phase fluid supply assembly and the chromatography column. In the second connection state, the first switching member connects the liquid phase fluid supply assembly, the sample liquid preparation channel, and the chromatography column, and connects the sample supply assembly and the waste liquid channel. Controlling the first liquid path assembly to drive the second cleaning reagent to flow through the first filter includes: controlling the first liquid path assembly to drive the second cleaning reagent to flow through the first filter in the first direction to clean the first filter and transport it to the waste liquid channel through the first switching member.

11. The liquid chromatography analyzer according to any one of claims 1 to 5 or claim 7, characterized in that, The liquid chromatography analyzer further includes a second filter provided between the first switching member and the chromatography column; The filtration accuracy of the second filter is greater than that of the first filter.

12. The liquid chromatography analyzer according to claim 11, wherein, The second filter includes a first filter layer and a second filter layer. The first filter layer is provided between the first switching member and the second filter layer. The pore diameter of the filtering holes of the first filter layer is in the range of 0.5 μm to 20 μm, and the pore diameter of the filtering holes of the second filter layer is in the range of 0.2 μm to 5 μm; and / or, The pore diameter of the filtering holes of the first filter is in the range of 10 μm to 200 μm.

13. The liquid chromatography analyzer according to claim 11, characterized in that, The liquid chromatography analyzer prestores preset replacement conditions. The first controller is further configured to: when the chromatography column meets the preset replacement conditions, output a warning signal to prompt to replace the chromatography column and the second filter simultaneously.

14. The liquid chromatography analyzer according to claim 11, characterized in that, The liquid chromatography analyzer further includes a fourth switching member. The second filter is switchably connected to the chromatography column and the waste liquid channel through the fourth switching member; The first controller is further configured with a second maintenance mode; In the second maintenance mode, the first controller is configured to perform the following actions: when the first switching member is in the first connected state, control the first liquid circuit component to drive the second cleaning reagent to be delivered to the sample liquid preparation channel through the first switching member, control the first switching member to switch to the second connected state, and control the second liquid circuit component to drive the second cleaning reagent in the sample liquid preparation channel to flow through the second filter, the fourth switching member, and the waste liquid channel in sequence, so as to use the second cleaning reagent to clean the second filter; Preferably, the liquid chromatograph analyzer pre-stores at least one of the following second preset conditions: a second preset time has elapsed since the second maintenance mode was last executed; The liquid chromatograph has cumulatively executed chromatographic analysis items for a second preset number of times since the second maintenance mode was last executed; Arriving at a second preset time point of a second preset cycle; obtaining information that the second filter needs to be cleaned and maintained according to the operation instruction received by the human-computer interaction device; The first controller is further configured to: execute the second maintenance mode or output information prompting execution of the second maintenance mode when any one of the second preset conditions is met.

15. A liquid chromatography analyzer, characterized in that, The invention comprises a sample supply component, a liquid-phase fluid supply component, a first switching member, a sample liquid preparation channel, a chromatography column, a detector and a first controller, wherein the first switching member has a switchable first connection state and a second connection state, in which the first switching member connects the sample supply component, the sample liquid preparation channel and the liquid-phase fluid supply component and the chromatography column, and in which the first switching member connects the liquid-phase fluid supply component, the sample liquid preparation channel and the chromatography column, and the chromatography column is arranged between the first switching member and the detector; The sample supply assembly includes a reaction container, a sample dispensing device and a first liquid circuit assembly, wherein the reaction container is used to provide a reaction site for the sample and the reaction reagent to form a sample liquid, the sample dispensing device is used to absorb the sample from the sample container and dispense at least part of the absorbed sample into the reaction container, the first liquid circuit assembly is used to drive the sample liquid in the reaction container to be transported to the sample liquid preparation channel through the first switching member when the first switching member is in the first connected state, the first liquid circuit assembly includes a first filter, the first filter is arranged between the reaction container and the first switching member to filter the sample liquid transported from the reaction container to the sample liquid preparation channel, and the first liquid circuit assembly is also used to drive a second cleaning reagent to clean the first filter; The liquid phase fluid supply assembly is used to: when the first switching member is in the second communication state, drive the sample liquid in the sample liquid preparation channel to be delivered to the chromatography column through the liquid phase fluid, and when the first switching member is in the second communication state, drive the liquid phase fluid to flow through the first switching member and the chromatography column in sequence; The chromatography column is used to adsorb the sample solution and to allow the liquid phase fluid to elute the sample solution to form a test solution to be measured; The detector is used to perform chromatographic analysis on the test solution flowing out of the chromatography column; The first controller is configured with a chromatographic analysis mode and a first maintenance mode; In the chromatographic analysis mode, the first controller is configured to perform the following actions: when the first switching member is in the first communication state, control the first liquid path assembly to drive the sample solution to flow from the reaction container through the first filter and be transported to the sample solution preparation channel through the first switching member, control the first switching member to switch to the second communication state, control the second liquid path assembly to drive the sample solution in the sample solution preparation channel to be transported to the chromatography column by the liquid phase fluid, control the first switching member to switch to the first communication state, control the second liquid path assembly to drive the liquid phase fluid to be transported to the chromatography column through the first switching member to elute the sample solution, and control the detector to perform chromatographic analysis on the test solution flowing out of the chromatography column; In the first maintenance mode, the first controller is configured to perform the following actions: control the first liquid path assembly to drive the second cleaning reagent to flow through the first filter to clean the first filter; Wherein, the second cleaning reagent is an alkaline reagent or a protease reagent.

16. The liquid chromatography analyzer according to claim 15, wherein, The sample dispensing device includes a sample needle, which is used to aspirate the sample from the sample container at the aspiration level and dispense at least a part of the aspirated sample into the reaction container and to aspirate the second cleaning reagent from the cleaning reagent container at the aspiration level and dispense the aspirated second cleaning reagent into the reaction container; In the first maintenance mode, the first controller is further configured to: before controlling the first liquid path assembly to drive the second cleaning reagent to flow through the first filter to clean the first filter, control the sample dispensing device to aspirate the second cleaning reagent from the cleaning reagent container at the aspiration level and dispense it into the reaction container; The controlling the first liquid path assembly to drive the second cleaning reagent to flow through the first filter includes: controlling the first liquid path assembly to drive the second cleaning reagent to flow from the reaction container along the first direction through the first filter; Preferably, the liquid chromatograph analyzer also includes a cleaning reagent placement component and a first transmission component, the cleaning reagent placement component is used to place the cleaning reagent container loaded with the second cleaning reagent, and the first transmission component is used to transfer the cleaning reagent container loaded with the second cleaning reagent from the cleaning reagent placement component to the liquid aspiration position; in the first maintenance mode, the first controller is also configured to: before controlling the sample dispensing device to absorb the second cleaning reagent from the cleaning reagent container located at the liquid aspiration position and dispense it into the reaction container, control the first transmission component to transfer the cleaning reagent container loaded with the second cleaning reagent from the cleaning reagent placement component to the liquid aspiration position.

17. A liquid chromatography analyzer, characterized in that, The invention comprises a sample supply component, a liquid-phase fluid supply component, a first switching member, a sample liquid preparation channel, a second filter, a chromatography column, a detector and a first controller, wherein the first switching member has a switchable first connection state and a second connection state, in which the first switching member connects the sample supply component, the sample liquid preparation channel and the liquid-phase fluid supply component and the second filter, and in which the first switching member connects the liquid-phase fluid supply component, the sample liquid preparation channel and the second filter, and the chromatography column is arranged between the second filter and the detector; The sample supply assembly includes a reaction container, a sample dispensing device and a first liquid circuit assembly, wherein the reaction container is used to provide a reaction site for the sample and the reaction reagent to form a sample liquid, the sample dispensing device is used to absorb the sample from the sample container and dispense at least part of the absorbed sample into the reaction container, the first liquid circuit assembly distributes the reaction reagent to the reaction container and drives the sample liquid in the reaction container to be transported to the sample liquid preparation channel through the first switching member when the first switching member is in the first connected state; The liquid-phase fluid supply assembly is used to: when the first switching member is in the second communication state, drive the sample liquid in the sample liquid preparation channel to be transported to the chromatography column through the second filter by means of the liquid-phase fluid; and when the first switching member is in the first communication state, drive the liquid-phase fluid to flow through the first switching member, the second filter and the chromatography column in sequence; The chromatography column is used to adsorb the sample liquid, and is used for the liquid phase fluid to elute the sample liquid to form a liquid to be tested; The detector is used to perform chromatographic analysis on the liquid to be tested flowing out of the chromatography column; The first controller is configured with a chromatographic analysis mode; In the chromatographic analysis mode, the first controller is configured to perform the following actions: when the first switching member is in the first communication state, control the first liquid path assembly to distribute a reaction reagent to the reaction vessel, control the sample dispensing device to distribute a sample to the reaction vessel, control the first switching member to switch to the second communication state, control the second liquid path assembly to drive the sample liquid in the sample liquid preparation channel through a liquid phase fluid to be delivered to the chromatography column via the second filter, control the first switching member to switch to the first communication state, control the second liquid path assembly to drive the liquid phase fluid to be delivered to the chromatography column via the first switching member and the second filter to elute the sample liquid, and control the detector to perform chromatographic analysis on the test liquid flowing out of the chromatography column; Wherein, the reaction reagent is a hemolytic agent containing a surfactant.

18. A liquid chromatography analyzer, characterized in that, It includes a sample supply assembly, a liquid phase fluid supply assembly, a first switching member, a sample liquid preparation channel, a second filter, a chromatography column, a detector and a first controller. The first switching member has a switchable first communication state and a second communication state. In the first communication state, the first switching member connects the sample supply assembly, the sample liquid preparation channel, and connects the liquid phase fluid supply assembly and the second filter. In the second communication state, the first switching member connects the liquid phase fluid supply assembly, the sample liquid preparation channel and the second filter. The chromatography column is disposed between the second filter and the detector; The sample supply assembly includes a reaction vessel, a sample dispensing device and a first liquid path assembly. The reaction vessel is used to provide a reaction place for the sample and the reaction reagent to prepare a sample liquid. The sample dispensing device is used to suck a sample from a sample container and dispense at least part of the sucked sample into the reaction vessel. The first liquid path assembly distributes the reaction reagent to the reaction vessel and is used to drive the sample liquid in the reaction vessel to be delivered to the sample liquid preparation channel via the first switching member when the first switching member is in the first communication state; The liquid phase fluid supply assembly is used for: when the first switching member is in the second communication state, driving the sample liquid in the sample liquid preparation channel through a liquid phase fluid to be delivered to the chromatography column via the second filter, and when the first switching member is in the first communication state, driving the liquid phase fluid to flow through the first switching member, the second filter and the chromatography column in sequence; The chromatography column is used to adsorb the sample liquid and is used to allow the liquid phase fluid to elute the sample liquid to form a test liquid; The detector is used to perform chromatographic analysis on the test liquid flowing out of the chromatography column; The first controller is configured with a chromatographic analysis mode; In the chromatographic analysis mode, the first controller is configured to perform the following actions: when the first switching member is in the first communication state, control the first liquid path assembly to distribute reaction reagents to the reaction vessel, control the sample dispensing device to distribute a sample to the reaction vessel, control the first switching member to switch to the second communication state, control the second liquid path assembly to drive the sample liquid in the sample liquid preparation channel through a liquid phase fluid to be transported to the chromatography column via the second filter, control the first switching member to switch to the first communication state, control the second liquid path assembly to drive the liquid phase fluid to be transported to the chromatography column via the first switching member and the second filter to elute the sample liquid, and control the detector to perform chromatographic analysis on the test liquid flowing out of the chromatography column; Wherein, in the reaction liquid obtained by the reaction of the reaction reagent and the sample, the proportion of the number of particles with a size in the range of 0 μm to 1 μm in the total number of particles is 40% to 90%, and the proportion of the number of particles with a size in the range of 1 μm to 2 μm in the total number of particles is 10% to 60%.

19. A sample analysis system, characterized in that, Comprising: A sample input device, which is at least used for placing a sample container loaded with a sample to achieve sample loading; A liquid chromatography analyzer, which is used to aspirate a sample from the sample container and perform chromatographic analysis on at least part of the aspirated sample; A second transmission assembly, and the second transmission track is used to transmit the sample container from the sample input device to the liquid chromatography analyzer; A second controller, which is used to control the second transmission assembly to transmit the sample container to the liquid chromatography analyzer; Wherein, the liquid chromatography analyzer includes a sample supply assembly, a liquid phase fluid supply assembly, a first switching member, a sample liquid preparation channel, a chromatography column, a detector and a first controller. The first switching member has a switchable first communication state and a second communication state. In the first communication state, the first switching member connects the sample supply assembly, the sample liquid preparation channel, and connects the liquid phase fluid supply assembly and the chromatography column. In the second communication state, the first switching member connects the liquid phase fluid supply assembly, the sample liquid preparation channel and the chromatography column. The chromatography column is arranged between the first switching member and the detector; The sample supply assembly includes a reaction container, a sample dispensing device and a first liquid circuit assembly, wherein the reaction container is used to provide a reaction site for the sample and the reaction reagent to form a sample liquid, the sample dispensing device is used to absorb the sample from the sample container and dispense at least part of the absorbed sample into the reaction container, the first liquid circuit assembly is used to drive the sample liquid in the reaction container to be transported to the sample liquid preparation channel through the first switching member when the first switching member is in the first connected state, the first liquid circuit assembly includes a first filter, the first filter is arranged between the reaction container and the first switching member to filter the sample liquid transported from the reaction container to the sample liquid preparation channel, and the first liquid circuit assembly is also used to drive a first cleaning reagent to clean the first filter, and to drive a second cleaning reagent to clean the first filter; The liquid-phase fluid supply assembly is used to: when the first switching member is in the second communication state, drive the sample liquid in the sample liquid preparation channel to be transported to the chromatography column through the liquid-phase fluid, and when the first switching member is in the first communication state, drive the liquid-phase fluid to flow through the first switching member and the chromatography column in sequence; The chromatography column is used to adsorb the sample liquid, and is used for the liquid phase fluid to elute the sample liquid to form a liquid to be tested; The detector is used to perform chromatographic analysis on the liquid to be tested flowing out of the chromatography column; The first controller is configured with a chromatographic analysis mode and a first maintenance mode; In the chromatography analysis mode, the first controller is configured to perform the following actions: when the first switching member is in the first connected state, control the first liquid circuit component to drive the sample liquid to flow from the reaction container through the first filter along a first direction and be delivered to the sample liquid preparation channel through the first switching member, and after the sample liquid is delivered to the sample liquid preparation channel, control the first liquid circuit component to drive the first cleaning reagent to flow through the first filter along a second direction to reversely flush the first filter with the first cleaning reagent, wherein the first direction and the second direction are two opposite directions; In the first maintenance mode, the first controller is configured to perform the following actions: control the first fluid circuit component to drive the second cleaning reagent to flow through the first filter to clean the first filter; The first cleaning reagent and the second cleaning reagent are two reagents with different components.

20. The sample analysis system according to claim 19, wherein The sample dispensing device comprises a sample needle, the sample needle is used to absorb the sample from the sample container located at the liquid aspiration position and dispense at least part of the absorbed sample into the reaction container, and is used to absorb the second cleaning reagent from the cleaning reagent container located at the liquid aspiration position and dispense the absorbed second cleaning reagent into the reaction container; In the first maintenance mode, the first controller is further configured to: before controlling the first liquid path component to drive the second cleaning reagent to flow through the first filter to clean the first filter, control the sample dispensing device to aspirate the second cleaning reagent from the cleaning reagent container located at the aspiration level and dispense it into the reaction container; The controlling the first liquid path component to drive the second cleaning reagent to flow through the first filter includes: controlling the first liquid path component to drive the second cleaning reagent to flow through the first filter from the reaction container along the first direction; Preferably, the sample analysis system further includes a cleaning reagent loading component for loading the cleaning reagent container containing the second cleaning reagent, and the second transfer component is further configured to transfer the cleaning reagent container containing the second cleaning reagent from the cleaning reagent loading component to the liquid chromatograph analyzer; in the first maintenance mode, the first controller is further configured to: before controlling the sample dispensing device to aspirate the second cleaning reagent from the cleaning reagent container located at the aspiration level and dispense it into the reaction container, control the second transfer component to transfer the cleaning reagent container containing the second cleaning reagent from the cleaning reagent loading component to the liquid chromatograph analyzer.

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