Planting method for promoting early survival and growth of panax notoginseng

Through the use of gibberellin and the use of cymellitin-treated seeds and Bacillus thuringiensis nutritional agents, the problems of low survival and growth efficiency in early Panax notoginseng were solved, and the early emergence and root development were improved, which reduced the planting cost.

CN120283620AActive Publication Date: 2025-07-11INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI
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Patent Information

Application Number
CN202510779013.1
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-06-12
Publication Date
2025-07-11
Estimated Expiration
2045-06-12

AI Technical Summary

Technical Problem

Among the existing Panax notoginseng planting methods, the early survival and growth efficiency of Panax notoginseng seedlings are low, resulting in a low seedling rate and increasing the planting cost.

Method used

The seeds were treated with gibberellin solution and closed fumigation of cyparin, combined with Bacillus thuringien fermentation nutrients, combined with appropriate shade management and fertilizer replenishment, to promote the early emergence and root development of Panax notoginseng seedlings.

Benefits of technology

It significantly improved the early emergence efficiency and seedling strengthening rate of Panax notoginseng, promoted root growth, and reduced planting costs.

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Abstract

The invention provides a planting method for promoting early survival and growth of pseudo-ginseng, and relates to the technical field of pseudo-ginseng planting. The panax notoginseng planting method mainly comprises the steps that panax notoginseng seeds are disinfected in the early stage, after hormone treatment, para-cymene with a certain concentration is adopted for fumigation treatment, and after follow-up sowing, bacillus thuringiensis is assisted to ferment pine needles and a sterilization solution of corn for irrigation. The method overcomes the defects in the prior art, can effectively improve the early-stage seedling emergence efficiency of panax notoginseng, ensures regular seedling emergence, promotes root growth of panax notoginseng seedlings, increases the strong seedling rate of panax notoginseng seedlings, and comprehensively improves the economic benefits of panax notoginseng planting.
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Description

Technical Field

[0001] The present invention relates to the technical field of Panax notoginseng planting, and specifically relates to a planting method for promoting the early survival and growth of Panax notoginseng. Background Art

[0002] Panax notoginseng is a perennial herbaceous plant of the Araliaceae family and the Panax genus, also known as Jinbuhuan, and is a traditional precious bulk Chinese medicinal material that has been artificially cultivated in China earlier. It is mainly produced in Wenshan, Yunnan. In recent years, there has also been sporadic cultivation in places such as Guangdong, Sichuan, Guizhou, and Hunan. Panax notoginseng uses its rhizome as medicine, with the curative effects of detumescence and pain relief, and dissipating stasis and stopping bleeding. It is mainly used to treat cardiovascular and cerebrovascular diseases, blood system diseases, and central nervous system diseases. Panax notoginseng generally needs to be planted for three years before it can be dug, and there is a serious continuous cropping obstacle.

[0003] The main planting method of Panax notoginseng is sowing. Before sowing, it is usually necessary to pretreat the seeds to break the dormancy period to promote the subsequent emergence of the seeds. And Panax notoginseng can generally be transplanted after growing for 1 year or more after sowing. The survival rate of transplantation is closely related to the strong seedling situation of the Panax notoginseng seedlings. Panax notoginseng seedlings with developed roots and thick stems have a higher survival rate in subsequent transplantation. Therefore, in order to improve the strong seedling rate of Panax notoginseng, it is often necessary to extend the seedling raising time of Panax notoginseng, which increases the cost of artificial planting of Panax notoginseng. Therefore, at the present stage, improving the early growth of Panax notoginseng seedlings and promoting the strong seedlings of Panax notoginseng are important directions for artificial planting. Summary of the Invention

[0004] Aiming at the deficiencies of the prior art, the present invention provides a planting method for promoting the early survival and growth of Panax notoginseng, which can effectively improve the early emergence efficiency of Panax notoginseng, ensure uniform emergence, and at the same time promote the root growth of Panax notoginseng seedlings, increase the strong seedling rate of Panax notoginseng seedlings, and comprehensively improve the economic benefits of Panax notoginseng planting.

[0005] To achieve the above objectives, the present invention is realized through the following technical solutions: A planting method for promoting the early survival and growth of Panax notoginseng, the planting method includes the following steps: S1. Seed pretreatment: After disinfecting the seeds, soak them in gibberellin solution to break the dormancy, and then place them in a sealed environment with cymene at a concentration of 0.2 - 0.5 g / m 3 for 1 - 2 h to obtain pretreated seeds for later use; S2. Nutrient agent preparation: Mix pine needles and corn, add 3 - 5 times the mass of water and crush, then inoculate Bacillus thuringiensis and ferment for 2 - 4 d, sterilize and then press and filter to obtain the nutrient agent for later use; S3. Sowing: Sow the above pretreated seeds into the prepared land, cover with a thin layer of soil, then pour thoroughly with the nutrient agent diluted 100 - 150 times, and cover with a plastic film or a layer of straw; S4. Seedling emergence management: After the seedling emergence rate exceeds 20%, pour and spray a nutrient agent diluted 100 - 150 times again. S5. Topdressing management: Apply compound fertilizer once at 4 - 5 months and 7 - 8 months after sowing.

[0006] Preferably, in step S1, the method of seed disinfection is to soak the seeds in a 50% carbendazim solution diluted 1000 times for 10 - 15 minutes and then take them out. The method of soaking with gibberellin solution is to control the concentration of the gibberellin solution at 100 - 200 mg / L and soak for 8 - 12 hours.

[0007] Preferably, in step S2, the mass ratio of pine needles to corn is 1 - 3∶1 - 3, and the amount of Bacillus thuringiensis inoculated is 2% - 3% of the total mass of pine needles and corn.

[0008] Preferably, the bacterial content of Bacillus thuringiensis in step S2 is 10 8 -10 10 CFU / g.

[0009] Preferably, the sterilization method in step S2 is to heat up to 119 - 121°C under a pressure of 0.4 - 0.6 Mpa and treat for 20 - 30 minutes.

[0010] Preferably, the sowing time in step S3 is from November of the current year to January of the next year. And immediately build a shading shed after sowing, control the shading rate at 30% - 40% from July to February, and 60% - 70% from March to June.

[0011] Preferably, the land prepared in advance in step S3 is to first plow and weed the land, then apply quicklime for disinfection, then use decomposed manure and superphosphate as the base fertilizer, and then rake the land fine and level it to make a ridge with a width of 1.2 - 1.5 m and a height of 20 - 30 cm. The width of the furrow is 30 - 40 cm, and the spacing for dibbling seeds is 5 - 7 cm.

[0012] Preferably, the application amount of the nutrient agent diluted 100 - 150 times poured and sprayed in step S4 is 200 - 300 kg / mu.

[0013] Preferably, the compound fertilizer in step S5 is 10 - 10 - 30 compound water-soluble fertilizer, and the single application amount is 3 - 5 kg / mu.

[0014] The present invention provides a planting method for promoting the early survival and growth of Panax notoginseng, and the advantages compared with the prior art are as follows: After disinfecting the Panax notoginseng seeds, they are fumigated with cymene in a closed space. Different from the existing research that uses cymene as a disinfection and antibacterial component to directly act on the soil, the method of air fumigation can promote the subsequent emergence uniformity of the seeds to a certain extent, and promote the development of the roots of the Panax notoginseng seedlings after early emergence to a certain extent. At the same time, the nutrient agent prepared by fermenting and sterilizing Bacillus thuringiensis is used for watering later, which further promotes the development of the roots of the Panax notoginseng seedlings, and promotes the root swelling and the growth of the stems and leaves, comprehensively improving the growth efficiency of the Panax notoginseng seedlings and increasing the strong seedling rate. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a control chart of the rooting situation of each group of Panax notoginseng seedlings on March 30, 2024 in the embodiment of the present invention; Figure 2 It is a control chart of the growth status of experimental group 6, experimental group 7, experimental group 4 and experimental group 8 when collected on November 21, 2024 in the embodiment of the present invention. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0016] To make the objectives, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are some, but not all, of the embodiments of the present invention. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without making creative efforts fall within the protection scope of the present invention.

[0017] The preservation number of Bacillus thuringiensis used in the following embodiments is CGMCC No. 20588, and the activity is 10 9 CFU / g.

[0018] Example 1

[0019] Panax notoginseng sowing experiment: 1. Selection and preliminary treatment of seed raw materials: Select the mature seeds of three-year-old Panax notoginseng, peel and wash them, then soak them in a 1000-fold diluted 50% carbendazim solution for 10 - 15 minutes, take them out and soak them in a gibberellin solution with a concentration of 150 mg / L for 10 hours to obtain disinfected seeds; Refer to Table 1 below to place the above disinfected seeds in a closed environment with different concentrations of cymene for fumigation treatment for 90 minutes to obtain different pre-treated seeds for standby; Table 1

[0020] 2. Preparation of nutrient agent: Nutrient agent 1: Mix pine needles and corn in equal mass ratio to obtain a mixture. Add 4 times the mass of clear water to the mixture, crush it, inoculate Bacillus thuringiensis accounting for 2% of the total mass of the mixture, and ferment for 3 days. Then, heat it to 120 °C under a pressure of 0.5 MPa for sterilization treatment for 25 minutes. After that, press and filter to obtain the filtrate as nutrient agent 1; Nutrient agent 2: Mix pine needles and corn in equal mass ratio to obtain a mixture. Add 4 times the mass of clear water to the mixture, crush it, inoculate Bacillus thuringiensis accounting for 2% of the total mass of the mixture, and ferment for 3 days. Then, press and filter to obtain the filtrate as nutrient agent 2; 3. Treatment of the sowing experimental field: Conduct Panax notoginseng planting experiments in a partitioned area of the Panax notoginseng planting base in Yanshan County, Yunnan. And this area has not been planted with Panax notoginseng for three years. Before sowing, first plow and weed the land, then broadcast 150 kg of quicklime per mu for disinfection. After 10 days, apply 1800 kg of decomposed manure and 50 kg of calcium phosphate per mu as basal fertilizer. Then, rake the land finely and level it to make ridges with a width of 1.2 - 1.5 m and a height of 20 - 30 cm. The ridge furrows are 30 - 40 cm wide. Then, set up a sunshade net on the land, control the shading rate at 30% - 40% from July to February, and 60% - 70% from March to June to obtain the treated planting field.

[0021] 4. Sowing experiment: On December 21, 2023, respectively, sow the above different pre-treated seeds in the above different planting fields at a spacing of 5 - 7 cm. After sowing, water thoroughly with the nutrient agent diluted 120 times and then cover a layer of straw. On February 1, 2024, uncover the film and then pour and sprinkle the 120 - fold diluted solution of nutrient agent 1 again at a dosage of 250 kg per mu. On April 26, 2024, apply supplementary fertilizer (5 kg per mu) with a 10 - 10 - 30 (10% N, 10% P2O5, 30% K2O) compound water-soluble fertilizer. Then, on July 22, 2024, apply supplementary fertilizer (3 kg per mu) with a 10 - 10 - 30 compound water-soluble fertilizer. The method of applying supplementary fertilizer is to dissolve the compound water-soluble fertilizer in water and then apply it by irrigation.

[0022] Set up multiple groups of experiments according to the selection of pre-treated seeds and nutrient agents in Table 2 below: Table 2

[0023] 5. Observation and recording of experimental results: 5.1 Observe and record the emergence rates of each group on February 1, February 15, and March 5, 2025. The specific results are shown in Table 3 below: Table 3

[0024] As can be seen from Table 3 above, the emergence rates of experimental groups 4-6 and experimental group 8 on February 1 were all above 35%. Therefore, fumigating seeds with a certain concentration of cymene can effectively improve the early emergence of seeds. When the concentration is relatively high, the emergence promotion efficiency decreases significantly. That is, by comparing experimental groups 3, 4, 8, and 7, the optimal fumigation concentration is 0.2-0.5 g / m 3 . In addition, using different nutrient agents also has a certain impact on the emergence rate. Among them, nutrient agent 1 has the best effect on promoting emergence compared to nutrient agent 2 and water.

[0025] 5.2. On March 30, 2024, 10 plants with a seedling height of 4.2-4.6 cm were randomly collected from each experimental group for observing root growth, and the number of lateral roots and average root length of each group were recorded. The specific results are shown in Table 4 and Figure 1 as follows: Table 4

[0026] As can be seen from the above table and Figure 1 , the root growth of experimental groups 4, 7, and 8 is the best.

[0027] 5.3. On November 21, 2024, 5 Panax notoginseng seedlings were randomly collected from each group, and the seedling height, stem diameter, and main root diameter of each group of Panax notoginseng seedlings were observed and recorded. The specific results are shown in Table 5 as follows: Table 5

[0028] As can be seen from the above table, using different nutrient agents has the greatest impact on the seedling height, stem diameter, and main root diameter of one-year-old Panax notoginseng seedlings. Among them, using nutrient agent 1 has the best effect.

[0029] According to the above experiments, combined with Figure 2 , the Panax notoginseng seedlings of experimental groups 6, 7, 4, and 8 were compared respectively. Among them, the main roots of the Panax notoginseng seedlings in experimental groups 4 and 8 had the best swelling effect, and the stems were stronger.

[0030] The above embodiments are only used to illustrate the technical solutions of the present invention, not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements on some of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A planting method for promoting the early survival and growth of Panax notoginseng, characterized in that, The planting method includes the following steps: S1. Seed pretreatment: After disinfecting the seeds, soak them in gibberellin solution to break dormancy, and then place them in a closed environment of cymene with a concentration of 0.2 - 0.5 g / m 3 for 1 - 2 h to obtain pretreated seeds for standby; S2. Nutrient agent preparation: Mix pine needles and corn, add 3 - 5 times the mass of water and crush, then inoculate Bacillus thuringiensis and ferment for 2 - 4 days. After sterilization, press and filter to obtain the nutrient agent for standby; S3. Sowing: Broadcast the pretreated seeds into the pre - prepared land, cover with a thin layer of soil, then pour thoroughly with the nutrient agent diluted 100 - 150 times, and cover with a plastic film or a layer of straw; S4. Seedling emergence management: After the seedling emergence rate exceeds 20%, pour and spray the nutrient agent diluted 100 - 150 times again; S5. Top - dressing management: Apply compound fertilizer once at 4 - 5 months and 7 - 8 months after sowing.

2. The planting method according to claim 1, characterized in that: In step S1, the method of seed disinfection is to soak the seeds in 50% carbendazim solution diluted 1000 times for 10 - 15 minutes and then take them out. The method of soaking with gibberellin solution is to control the concentration of the gibberellin solution at 100 - 200 mg / L and soak for 8 - 12 hours.

3. The planting method according to claim 1, characterized in that: In step S2, the mass ratio of pine needles to corn is 1 - 3∶1 - 3, and the amount of Bacillus thuringiensis inoculated is 2% - 3% of the total mass of pine needles and corn.

4. The planting method according to claim 1, wherein: The bacterial content of Bacillus thuringiensis in the step S2 is 10 8 -10 10 CFU / g.

5. The planting method according to claim 1, characterized in that: In step S2, the sterilization method is to heat up to 119 - 121 °C under a pressure of 0.4 - 0.6 Mpa and treat for 20 - 30 minutes.

6. The planting method according to claim 1, wherein: In step S3, the sowing time is from November of the current year to January of the next year. After sowing, set up a shading shed in time, control the shading rate at 30% - 40% from July to February, and 60% - 70% from March to June.

7. The planting method according to claim 1, wherein: In step S3, the pre - prepared land is first plowed and weeded, then lime is applied for disinfection, then decomposed manure and superphosphate are used as base fertilizers, and then the land is harrowed and leveled to form a ridge with a width of 1.2 - 1.5 m and a height of 20 - 30 cm. The width of the ridge ditch is 30 - 40 cm, and the spacing of broadcast sowing of seeds is 5 - 7 cm.

8. The planting method according to claim 6, characterized in that: In step S4, the amount of the nutrient agent diluted 100 - 150 times for pouring and spraying is 200 - 300 kg / mu.

9. The planting method according to claim 6, wherein: In step S5, the compound fertilizer is 10 - 10 - 30 water - soluble compound fertilizer, and the single - time dosage is 3 - 5 kg / mu.

Citation Information

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