Bactericidal composition and application thereof

Abstract

The invention relates to a bactericidal composition and application thereof, the bactericidal composition comprises an active component A and an active component B. The active component A is a compound represented by formula (I), and the active component B is any one of bromothalonil, JS399-19 and prochloraz. The mass ratio of the active component A to the active component B is (1: 36)-(34: 1). The bactericidal composition or the preparation thereof can enhance the pesticide effect, reduce the dosage, improve the control effect and delay the development of pesticide resistance.

Description

Technical Field

[0001] The present invention relates to the field of pesticide fungicides, and particularly to a bactericidal composition and its application. Background Art

[0002] The compound of formula (I) is a quinoline carboxamide fungicide developed by Syngenta. It has a broad control spectrum, is safe and highly effective, and can effectively control a variety of fungal diseases. CAS registration number: 2132414-06-1 (racemic 2132414-04-9), English chemical name N-(2R)-[1,3-dimethyl-1-(phenylmethyl)butyl]-8-fluoro-3-quinolinecarboxamide, and the chemical structure is as follows:

[0003]

[0004] Jozamycin is a broad-spectrum, highly effective and low-toxic fungicide that can inhibit and eradicate the growth of fungi, bacteria and algae, and has a good control effect on crop diseases.

[0005] Phenamacril belongs to the 2-cyanoacrylate fungicides. It is a selective fungicide and a novel fungicide with a unique mode of action. It is effective against diseases caused by Fusarium. Phenamacril is absorbed through the roots and has upward translocality on the leaves.

[0006] Prochloraz is a broad-spectrum fungicide with acropetal conductive activity. Its mechanism of action is to inhibit the biosynthesis of ergosterol, thereby damaging the function of the cell membrane of the fungal body and playing a role. It has obvious control effects on diseases caused by ascomycetes and deuteromycetes in a variety of crops.

[0007] In recent years, with the annual expansion of the chili planting area and the increasing number of introduced varieties year by year, combined with continuous monoculture for a long time, the incidence area of chili anthracnose has been continuously expanding, resulting in the rise of anthracnose, which was originally a minor disease, to a major disease, causing a reduction in chili production and bringing huge losses to production. Chili anthracnose is caused by the infection of pathogens of the genus Colletotrichum in the Deuteromycotina subphylum. It has a wide distribution range, rapid onset, great harm to crops, and causes a lot of economic losses in China. Moreover, it has developed resistance to a variety of fungicides, and the efficacy of conventional chemical control has been gradually declining in recent years. Chemical pesticides are still the main force in controlling crop diseases at present. However, the long-term use of pesticides will cause cross-resistance, resulting in a slow reduction in the control effect. The main means to solve this problem is to compound two or more pesticides to produce a synergistic effect and improve the efficacy. Developing new fungicides is expensive and has a long cycle. In contrast, the development and research of high-efficiency, low-toxicity, and low-residue compounding and mixing have received attention at home and abroad due to their small investment and short research and development cycle, and the research and development efforts have been increased. The combination of the compound of formula (I) with bromothalonil, phenamacril, and prochloraz has not been reported yet. Summary of the Invention

[0008] Based on the above situation, the object of the present invention is to provide a bactericidal composition and its preparation, which are mainly used for controlling plant fungal diseases. The composition or its preparation can enhance the efficacy, reduce the dosage of pesticides, and at the same time can extend the duration of efficacy and delay the development of drug resistance.

[0009] In order to achieve the above object, the following technical solutions are provided: A bactericidal composition contains active ingredient A and active ingredient B. The active ingredient A is the compound shown in formula (I):

[0010] The active ingredient B is any one of bromothalonil, phenamacril, and prochloraz;

[0011] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 1:36 to 34:1.

[0012] Furthermore, the mass ratio of the compound of formula (I) to bromothalonil is 1:32 to 32:1;

[0013] Furthermore, the mass ratio of the compound of formula (I) to bromothalonil is 1:32, 1:24, 1:10, 1:6, 1:3, 1:1, 3:1, 6:1, 10:1, 18:1, 32:1;

[0014] Furthermore, the mass ratio of the compound of formula (I) to bromothalonil is 1:24 to 32:1;

[0015] Further, the mass ratio of the compound of formula (I) to cymoxanil is 1:24, 1:10, 1:6, 1:3, 1:1, 3:1, 6:1, 10:1, 18:1, 32:1;

[0016] Further, the mass ratio of the compound of formula (I) to phenamacril is 1:30 to 25:1;

[0017] Further, the mass ratio of the compound of formula (I) to phenamacril is 1:30, 1:25, 1:18, 1:12, 1:8, 1:5, 5:4, 5:1, 10:1, 15:1, 25:1;

[0018] Further, the mass ratio of the compound of formula (I) to phenamacril is 1:25 to 25:1;

[0019] Further, the mass ratio of the compound of formula (I) to phenamacril is 1:25, 1:18, 1:12, 1:8, 1:5, 5:4, 5:1, 10:1, 15:1, 25:1;

[0020] Further, the mass ratio of the compound of formula (I) to prochloraz is 1:36 to 34:1;

[0021] Further, the mass ratio of the compound of formula (I) to prochloraz is 1:36, 1:25, 1:15, 2:11, 3:8, 2:1, 8:3, 11:2, 10:1, 20:1, 34:1;

[0022] Further, the mass ratio of the compound of formula (I) to prochloraz is 1:25 to 20:1;

[0023] Further, the mass ratio of the compound of formula (I) to prochloraz is 1:25, 1:15, 2:11, 3:8, 2:1, 8:3, 11:2, 10:1, 20:1;

[0024] Further, based on the total weight of the bactericidal composition being 100 wt%, the sum of the contents of active ingredient A and active ingredient B in the bactericidal composition is 1 to 90 wt%;

[0025] Further, based on the total weight of the bactericidal composition being 100 wt%, the sum of the contents of active ingredient A and active ingredient B in the bactericidal composition is preferably 5 to 80 wt%;

[0026] Further, the bactericidal composition contains, in addition to the active ingredient, agriculturally acceptable auxiliary ingredients selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreezing agents, defoaming agents, solvents, preservatives, stabilizers, synergists, binders or carriers;

[0027] Further, the wetting agent is selected from one or more of alkyl benzene sulfonates, alkyl naphthalene sulfonates, lignin sulfonates, sodium dodecyl sulfate, sodium dioctyl sulfosuccinate, α-olefin sulfonates, alkylphenol polyoxyethylene ethers, castor oil polyoxyethylene ethers, alkylphenol ethoxylates, fatty alcohol ethoxylates, sodium fatty alcohol polyoxyethylene ether sulfate, silkworm excrement, Chinese honey locust powder, sapindus powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0028] Further, the dispersant is selected from one or more of lignin sulfonates, alkyl naphthalene sulfonate formaldehyde condensates, naphthalene sulfonates, triphenylvinylphenol ethoxylate phosphates, fatty alcohol ethoxylates, alkylphenol polyoxyethylene ethers, alkylphenol polyoxyethylene ether methyl ether condensate sulfates, fatty amine polyoxyethylene ethers, glycerol fatty acid ester polyoxyethylene ethers, polycarboxylates, polyacrylates, phosphates, EO-PO block copolymers and EO-PO graft copolymers; and / or

[0029] Further, the emulsifier is selected from one or more of calcium dodecylbenzene sulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether and alkylphenol ether phosphate; and / or

[0030] Further, the thickener is selected from one or more of xanthan gum, organic bentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and silica white; and / or

[0031] Further, the disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and / or

[0032] Further, the antifreezing agent is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons and inorganic salts; and / or

[0033] Further, the defoaming agent is selected from one or more of C 10 -C 20 saturated fatty acid compounds, silicone oils, silicone compounds, C8-C 10 fatty alcohols; and / or

[0034] Further, the solvent is selected from one or more of benzene, toluene, xylene, durene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel oil, solvent naphtha, vegetable oil (such as soybean oil, corn oil, rapeseed oil, palm oil, etc.), vegetable oil derivatives, and water; and / or

[0035] Further, the preservative is selected from one or more of propionic acid, sodium propionate, sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate, carbopol, and 1,2-benzisothiazolin-3-one; and / or

[0036] Further, the stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, diatomaceous earth, bentonite, attapulgite, silica white, talc powder, montmorillonite, and starch; and / or

[0037] Further, the synergist is selected from synergistic phosphorus and synergistic ether; and / or

[0038] Further, the carrier is selected from one or more of ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils, and vegetable oil derivatives.

[0039] Further, the dosage form of the bactericidal composition is selected from solid preparations and / or liquid preparations and / or seed treatment preparations;

[0040] The solid preparations include powders, granules, balls, tablets, strips, wettable powders, oil-dispersed powders, milk powders, water-dispersible granules, milk granules, water-dispersible tablets, soluble powders, soluble tablets, or soluble granules;

[0041] The liquid preparations include soluble solutions, soluble sols, oils, film-forming oils, emulsifiable concentrates, latexes, dispersible liquids, pastes, emulsions, oil emulsions, microemulsions, fats, suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspension emulsions, microcapsule suspension-suspensions, microcapsule suspension-emulsions, or microcapsule suspension-suspension emulsions;

[0042] Further, the powder is a free-flowing powdery preparation suitable for dusting or spreading containing the active ingredient;

[0043] Further, the granule is a free-flowing granular preparation with a certain particle size range containing the active ingredient;

[0044] Further, the ball is a spherical preparation containing the active ingredient (generally with a diameter greater than 6 mm);

[0045] Further, the tablet is a sheet preparation containing an active ingredient with a certain shape and size (usually having two flat surfaces or convex surfaces, and the distance between the two surfaces is less than the diameter);

[0046] Further, the strip preparation is a strip or rod-shaped preparation containing an active ingredient (generally several centimeters in length and a few millimeters in width / diameter, that is, the length is greater than the diameter / width);

[0047] Further, the wettable powder is a powdery preparation in which the active ingredient is dispersed into a suspension in water;

[0048] Further, the oil-dispersible powder is a powdery preparation in which the active ingredient is dispersed into a suspension in an organic solvent;

[0049] Further, the milk powder is a powdery preparation in which the active ingredient is dissolved in an organic solvent and wrapped in soluble or insoluble inert ingredients, and forms an oil-in-water emulsion when dispersed in water;

[0050] Further, the water-dispersible granule is a granular preparation that disintegrates in water and the active ingredient is dispersed into a suspension;

[0051] Further, the milk granule is a granular preparation in which the active ingredient is dissolved in an organic solvent and wrapped in soluble or insoluble inert ingredients, and forms an oil-in-water emulsion when dispersed in water;

[0052] Further, the water-dispersible tablet is a sheet preparation that disintegrates in water and the active ingredient is dispersed into a suspension;

[0053] Further, the soluble powder is a powdery preparation in which the active ingredient forms a true solution in water and may contain inert ingredients insoluble in water;

[0054] Further, the soluble granule is a granular preparation in which the active ingredient forms a true solution in water and may contain inert ingredients insoluble in water;

[0055] Further, the soluble tablet is a sheet preparation in which the active ingredient forms a true solution in water and may contain inert ingredients insoluble in water;

[0056] Further, the soluble solution is a liquid preparation that is diluted with water into a transparent or semi-transparent liquid containing an active ingredient and may contain inert ingredients insoluble in water;

[0057] Further, the soluble sol is a colloidal preparation in which the active ingredient forms a true solution when diluted with water;

[0058] Further, the oil preparation is a liquid preparation that is diluted (or not diluted) with an organic solvent into a homogeneous liquid containing an active ingredient;

[0059] Further, the spreading oil agent is an oil agent that can automatically spread into an oil film on the water surface and contains active ingredients;

[0060] Further, the emulsifiable concentrate is a homogeneous liquid preparation that can be diluted and dispersed with water into an emulsion and contains active ingredients;

[0061] Further, the latex is a latex preparation that can be diluted and dispersed with water into an emulsion and contains active ingredients;

[0062] Further, the dispersible concentrate is a homogeneous liquid preparation that can be diluted and dispersed with water into a suspension and contains active ingredients;

[0063] Further, the paste is a water-based paste preparation that contains active ingredients and can form a film, and is generally used directly;

[0064] Further, the aqueous emulsion is a liquid preparation in which the active ingredient (or its organic solution) forms an emulsion in water;

[0065] Further, the oil emulsion is a liquid preparation in which the active ingredient (or its aqueous solution) forms an emulsion in oil;

[0066] Further, the microemulsion is a liquid preparation in which the active ingredient forms a transparent or semi-transparent microemulsion in water, and can be used directly or after dilution with water;

[0067] Further, the fat agent is an oily or fatty-based viscous preparation that contains active ingredients and is generally used directly;

[0068] Further, the suspending agent is a stable suspension preparation in which the active ingredient is dispersed as solid particles in water, and is generally diluted with water before use;

[0069] Further, the microcapsule suspension is a stable suspension preparation in which microcapsules containing active ingredients are dispersed in a liquid;

[0070] Further, the oil suspending agent is a stable suspension preparation in which the active ingredient is dispersed as solid particles in a liquid, and is generally diluted with an organic solvent before use;

[0071] Further, the dispersible oil suspending agent is a stable suspension preparation in which the active ingredient is dispersed as solid particles in a non-aqueous medium, and is generally diluted with water before use;

[0072] Further, the suspoemulsion is a heterogeneous liquid preparation in which the active ingredient is stably dispersed in the continuous water phase in the form of solid particles and tiny water-insoluble droplets;

[0073] Further, the microcapsule suspension-suspending agent is a stable suspension preparation in which the active ingredient is dispersed as microcapsules and solid particles in water;

[0074] Further, the microcapsule suspension-emulsion is a heterogeneous liquid preparation in which the active ingredient is stably dispersed in the continuous aqueous phase in the form of microcapsules and tiny droplets.

[0075] Further, the microcapsule suspension-suspension emulsion is a heterogeneous liquid preparation in which the active ingredient is stably dispersed in the continuous aqueous phase in the form of microcapsules, solid particles and tiny droplets.

[0076] Further, the bactericidal composition can be prepared into a pesticide-acceptable formulation dosage form, and the formulation dosage form is a microemulsion, an emulsion in water, a suspension, a dispersible oil suspension, a soluble solution, an emulsifiable concentrate, a suspension-suspension emulsion, a microcapsule suspension, a water dispersible granule, a wettable powder, a granule, a seed treatment suspension, a seed treatment dry powder.

[0077] Further, the formulation dosage form is a microemulsion, an emulsifiable concentrate, a suspension, an emulsion in water, a water dispersible granule, a wettable powder, a seed treatment suspension.

[0078] The present invention also discloses the application of a bactericidal composition as described above in the prevention and control of plant diseases.

[0079] The compound of formula (I) has a synergistic effect when used in combination with bromothalonil, cyprodinil, prochloraz against Blumiria, Erysiphe, Gibberella, Sclerotinia, Botryotinia, Puccinia, Tilletia, Gymnosporzngium, Phakopsora, Ustilago, Rhizoctonia, Verticillium, Alternaria, Pyricularia, Botrytis, Cercospora, Fusarium, Colletotrichum, Rhizoctonia, Sclerotium, etc., especially having a synergistic effect on Colletotrichum within the mass ratio range of 1:32 to 30:1.

[0080] Further, the plant disease is a plant disease caused by fungi or bacteria.

[0081] Further, the plant disease is a plant disease caused by fungi.

[0082] Further, the plant disease caused by fungi is pepper anthracnose.

[0083] The present invention has the following beneficial effects:

[0084] 1) The bactericidal composition increases the bactericidal activity, has a synergistic effect on the target at a certain mass ratio, and has the effects of reducing the amount of pesticides used, reducing the use cost and reducing the environmental load;

[0085] 2) The bactericidal composition is safe and efficient, safe for crops, non-target organisms, beneficial organisms and natural enemies, and has the effect of increasing production and ensuring harvest. Specific embodiments

[0086] In order to make the technical solutions, objectives and advantages of the present invention clearer and more understandable, the present invention is described by the following specific embodiments, but the present invention can be implemented in various forms and should not be limited by the embodiments described herein.

[0087] Indoor bioassay of pepper anthracnose

[0088] Test basis: Refer to the Pesticide Indoor Bioassay Test Guidelines Fungicides Part 2: Inhibiting the Mycelial Growth of Pathogenic Fungi Petri Dish Method NY / T 1156.2 - 2006 of the Agricultural Industry Standard of the People's Republic of China.

[0089] Test strains: The anthracnose was isolated from the typical anthracnose spots on pepper fruits in Shouguang, Weifang. It was isolated, purified, cultured and preserved by the conventional tissue isolation method. The pathogen was identified as Colletotrichum nigrum. Before the test, the strains were transferred to PDA medium for subculture. The culture temperature was 26 - 27 °C and the culture time was about 5 days.

[0090] Test agents: 95% bromothalonil technical, 95% cyenopyrafen technical, 95% prochloraz technical, the technical of the compound of formula (I). The above agents were all provided by the R & D center of the group.

[0091] Agent preparation: The test technicals were first dissolved in acetone and then diluted with 0.1% Tween 80 aqueous solution. Single-agent mother liquors were prepared respectively, and 5 series of mass concentrations were set according to the mixing purpose and agent activity.

[0092] After melting the PDA medium with a microwave oven and cooling it to about 50 °C, according to the principle of from low concentration to high concentration, 1 mL of the prepared test liquid and 9 mL of PDA medium were added to a Petri dish with a diameter of 9 cm and mixed evenly to prepare the corresponding concentration of medicated plates.

[0093] Inoculation: Under aseptic conditions, use a punch with a diameter of 6 mm to punch out disks of the activated pathogenic fungus from the well-cultured pathogenic bacteria. After the culture medium containing the drug has solidified, place the disks at the center of the culture medium. Finally, seal the petri dish with sealing film and then incubate it in an incubator at 27°C with a relative humidity of 70%. At the same time, set up a treatment without the drug as a blank control, and repeat each treatment 3 times.

[0094] Investigation: Investigate the growth of the pathogenic bacteria hyphae based on the growth of the hyphae in the blank control petri dish. After 5 days of incubation, use a caliper to measure the colony diameter in millimeters (mm). Measure the diameter of each colony vertically once using the cross method and take the average value.

[0095] Calculate the hyphal growth inhibition rate according to the following formula, with the unit being percentage (%), and retain two decimal places in the calculation result.

[0096] D = D1 - D2

[0097] D —— Colony growth diameter;

[0098] D1 —— Colony diameter;

[0099] D2 —— Disk diameter.

[0100] I = (D0 - D t ) / D0 * 100

[0101] In the formula:

[0102] I —— Hyphal growth inhibition rate;

[0103] D0 —— Colony growth diameter of the blank control;

[0104] D t —— Colony growth diameter of the treatment with the drug.

[0105] Statistical analysis: Conduct a regression analysis based on the logarithm values of the concentrations of each drug and the corresponding probit values of the hyphal growth inhibition rate (analyzed using DPS statistical analysis software), calculate the EC 50 equivalent values, correlation coefficient R, and evaluate the activity of the test drugs on the biological test materials.

[0106] Sun Yunpei method: Evaluate the synergistic effect of drug mixtures based on the co-toxicity coefficient (CTC). A co-toxicity coefficient CTC ≥ 120 for the mixture indicates a synergistic effect; CTC ≤ 80 indicates an antagonistic effect; 80 < CTC < 120 indicates an additive effect.

[0107] The co-toxicity coefficient (CTC value) of the mixture is calculated according to the following formula:

[0108]

[0109] In the formula:

[0110] ATI - Measured toxicity index of the mixture

[0111] S - EC of the standard fungicide 50 , with the unit of milligram per liter (mg / L);

[0112] M - EC of the mixture 50 , with the unit of milligram per liter (mg / L).

[0113] TTI = TI A *P A +TI B *P B

[0114] In the formula:

[0115] TTI - Theoretical toxicity index of the mixture

[0116] TI A - Toxicity index of chemical A

[0117] P A - Percentage content of chemical A in the mixture, with the unit of percentage (%).

[0118] TI B - Toxicity index of chemical B

[0119] P B - Percentage content of chemical B in the mixture, with the unit of percentage (%).

[0120]

[0121] In the formula:

[0122] CTC - Co - toxicity coefficient

[0123] ATI - Measured toxicity index of the mixture

[0124] TTI - Theoretical toxicity index of the mixture.

[0125] Results of indoor bio - activity determination:

[0126] The results in Table 1 show that the EC 50 of the compound of formula (I) against Colletotrichum capsici is 2.9582 mg / L, and the EC 50 of bromothalonil against Colletotrichum capsici is 0.2862 mg / L.

[0127] The compound of formula (I) and cymoxanil show additive or synergistic effects in the mass ratio range of 1:32 to 32:1. Among them, when the mass ratio is 1:18 to 32:1, the co-toxicity coefficient > 120, showing a synergistic effect. The co-toxicity coefficient value of the compound of formula (I) and cymoxanil = 1:1 is the largest, which is 191.9480, and the EC 50 is 0.2719 mg / L, with a significant synergistic effect.

[0128] Table 1 Determination results of the synergistic effect of different ratios of the compound of formula (I) and cymoxanil on Colletotrichum capsici

[0129]

[0130]

[0131] Table 2 results show that the EC 50 of the compound of formula (I) for controlling Colletotrichum capsici is 3.0029 mg / L, and the EC 50 of phenamacril for controlling Colletotrichum capsici is 25.9952 mg / L. In the range of the mass ratio of the compound of formula (I) and phenamacril from 1:30 to 25:1, the co-toxicity coefficient > 120, showing a synergistic effect. The co-toxicity coefficient value of the compound of formula (I) and phenamacril = 1:5 is the largest, which is 188.1187, and the EC 50 is 6.0715 mg / L, with a significant synergistic effect.

[0132] Table 2 Determination results of the synergistic effect of different ratios of the compound of formula (I) and phenamacril on Colletotrichum capsici

[0133]

[0134] Table 3 results show that the EC 50 of the compound of formula (I) for controlling Colletotrichum capsici is 3.0090 mg / L, and the EC 50 of prochloraz for controlling Colletotrichum capsici is 10.3421 mg / L. The compound of formula (I) and prochloraz show additive or synergistic effects in the mass ratio range of 1:36 to 34:1. Among them, when the mass ratio is 1:25 to 20:1, the co-toxicity coefficient > 120, showing a synergistic effect. The co-toxicity coefficient value of the compound of formula (I) and prochloraz = 2:1 is the largest, which is 185.7448, and the EC 50 is 3.0722 mg / L, with a significant synergistic effect.

[0135] Table 3 Determination results of the synergistic effect of different ratios of the compound of formula (I) and prochloraz on Colletotrichum capsici

[0136]

[0137] Formulation examples

[0138] Preparation Example 1:

[0139] 40% Wettable Powder of Compound (I)·Bromothalonil (20:20)

[0140] Formulation: 20% of Compound (I), 20% of bromothalonil, 5% of sodium polycarboxylate, 5% of dispersant NNO, 5% of sodium alkyl polyoxyethylene ether sulfonate, 3.5% of sodium dodecyl sulfate, 5% of bentonite, and kaolin to make up the balance;

[0141] Preparation method: Mix the active ingredient, other functional auxiliaries and fillers according to the formula ratio, stir evenly in a stirring kettle, and pulverize and mix evenly several times by an air flow pulverizer to obtain the wettable powder of the composition of the present invention.

[0142] Preparation Example 2:

[0143] 14% Emulsifiable Concentrate of Compound (I)·Bromothalonil (7:7)

[0144] Formulation: 7% of Compound (I), 7% of bromothalonil, 1.5% of BHT, 5% of epoxidized soybean oil, 10% of propylene carbonate, 15% of acetophenone, 5% of cyclohexanone, 3.5% of calcium dodecylbenzenesulfonate, 10% of fatty alcohol polyoxyethylene ether, and methyl oleate to make up the balance;

[0145] Preparation method: Add the active ingredient to the cosolvent according to the formula ratio of the example, and add surfactants and other functional auxiliaries thereto, and stir and mix evenly in a stirring and mixing kettle to obtain the emulsifiable concentrate.

[0146] Preparation Example 3:

[0147] 14% Water Emulsion of Compound (I)·Prochloraz (10:4)

[0148] Formulation: 10% of Compound (I), 4% of prochloraz, 12% of mesitylene, 10% of cyclohexanone, 1% of calcium dodecylbenzenesulfonate, 0.5% of sodium fatty alcohol polyoxyethylene ether sulfate, 5% of EO / PO block copolymer, 5% of glycerol, 1.5% of glycerin, 0.1% of silicone defoamer, 0.2% of xanthan gum, 0.1% of sodium benzoate, and deionized water to make up the balance;

[0149] Preparation method: According to the formula ratio of the example, dissolve the active ingredient in the solvent and add the emulsifier to form a homogeneous oil phase, mix deionized water and antifreeze together to form a homogeneous water phase; under high-speed shearing, add the water phase to the oil phase to form a well-dispersed water emulsion preparation.

[0150] Preparation Example 4:

[0151] 25% Suspension Concentrate of Compound (I)·Bromothalonil (15:10)

[0152] Formulation: 15% of the compound of formula (I), 10% of bromothalonil, 3% of naphthalene sulfonate formaldehyde condensate, 2% of lignosulfonic acid, 2% of sodium lignosulfonate, 1% of sodium dioctyl sulfosuccinate, 1% of magnesium aluminum silicate, 0.5% of fumed silica, 0.5% of polyvinylpyrrolidone, 0.25% of xanthan gum, 5% of ethylene glycol, 0.02% of benzisothiazolinone, 0.5% of silicone defoamer, and deionized water to make up the balance;

[0153] Preparation method: According to the formulation ratio, the active ingredient, surfactant and other functional auxiliaries are placed in the reaction kettle in turn, mixed evenly with water, subjected to high-speed shearing and wet grinding, and finally homogenized and filtered to obtain the suspension.

[0154] Preparation Example 5:

[0155] 28% Compound of formula (I)·Phenamacril Suspension Concentrate (24:4)

[0156] Formulation: 24% of the compound of formula (I), 4% of phenamacril, 1.5% of isomeric alcohol polyoxyethylene ether, 4% of polyether, 3% of styrylphenol polyoxyethylene ether phosphate, 1% of sodium polycarboxylate, 1% of magnesium aluminum silicate, 0.25% of xanthan gum, 5% of ethylene glycol, 0.02% of benzisothiazolinone, 0.5% of silicone defoamer, and deionized water to make up the balance;

[0157] Preparation method: The same as Preparation Example 4.

[0158] Preparation Example 6:

[0159] 30% Compound of formula (I)·Prochloraz Suspension Concentrate (20:10)

[0160] Formulation: 10% of the compound of formula (I), 10% of prochloraz, 1% of isomeric alcohol polyoxyethylene ether, 3% of sodium lignosulfonate, 1% of sodium polycarboxylate, 3.5% of sodium naphthalene sulfonate formaldehyde condensate, 1% of magnesium aluminum silicate, 0.25% of xanthan gum, 0.5% of fumed silica, 0.5% of polyvinylpyrrolidone, 5% of ethylene glycol, 0.02% of benzisothiazolinone, 0.5% of silicone defoamer, and deionized water to make up the balance;

[0161] Preparation method: The same as Preparation Example 4.

[0162] Field efficacy test

[0163] Field efficacy test against Colletotrichum capsici

[0164] Test site: Qingzhou City, Weifang City, Shandong Province;

[0165] Test crops: Chili peppers (thread peppers), planted outdoors; the planting environment, water and fertilizer conditions, and growth status of the chili peppers in the field are all the same, and they are more susceptible to diseases. The conditions meet the requirements of field trials.

[0166] Test time: On July 5, 2023, at the initial stage of the occurrence of Colletotrichum capsici, the medicine was applied by the method of uniform spraying on the stems and leaves of the plants.

[0167] Test agents and dosages:

[0168] Table 4 Comparison table of dosages for field trials

[0169] Treatment Agent <![CDATA[Dosage g a.i / hm 2 > A1 40% Compound of Formula (I)·Junduqing Wettable Powder (20:20) 100 A2 28% Compound of Formula (I)·Cyenopyraf Suspension Concentrate (24:4) 100 A3 30% Compound of Formula (I)·Prochloraz Suspension Concentrate (20:10) 100 A4 30% Compound of Formula (I) Suspension Concentrate 200 A5 25% Junduqing Wettable Powder 180 A6 25% Prochloraz EC 250 A7 25% Cyenopyraf Suspension Concentrate 300 A8 Water Control /

[0170] Test treatments: Single agents and a water control were set up. The interval between the two applications was 7 days, and each treatment was repeated 4 times. The plot area was divided into 20 m 2 , and they were arranged in a randomized block design. Ensure that rain, strong wind, and extreme high temperature are avoided during spraying. The spraying equipment was a 3WBD-18L knapsack electric sprayer, and the spraying liquid volume was 700 L / hm 2 .

[0171] Investigation method: Disease investigations were carried out before the first application, 7 days after the first application, and 7 days after the last application, and the number of diseased fruits and relative grade values were counted. The grading method referred to "GB / T 17980.33-2000 Pesticide field efficacy test guidelines (1) Fungicides for controlling Colletotrichum capsici", and the specific grading was as follows:

[0172] A total of 9 grades:

[0173] Grade 0, no disease;

[0174] Grade 1, the proportion of the lesion area on the fruit ≤ 2%;

[0175] Grade 3, the proportion of the lesion area on the fruit is 3% - 8%;

[0176] Grade 5, the proportion of the lesion area on the fruit is 9% - 15%;

[0177] Grade 7, the proportion of the lesion area on the fruit is 16% - 25%;

[0178] Grade 9, the proportion of the lesion area on the fruit > 25%;

[0179] Calculation methods for disease index and efficacy:

[0180]

[0181] The DPS software was used to conduct biostatistical analysis by the Duncan's new multiple range test (DMRT) on the test data, and the significance of differences was evaluated.

[0182] Results of field efficacy trials:

[0183] The results showed that the 40% compound (I)·bromothalonil wettable powder (20:20), 28% compound (I)·cyenopyrac suspension concentrate (24:4), and 30% compound (I)·prochloraz suspension concentrate (20:10) had good control effects on Colletotrichum capsici. Seven days after the first application, their control effects were 83.24%, 81.35%, and 78.91% respectively; there were significant differences between the compound preparations and other single agents.

[0184] Table 5 Results of field efficacy trials (7 days after the first application)

[0185]

[0186] Note: The data in the table are the average values of 4 replicates; lowercase letters indicate significant differences between treatments (p < 0.05)

[0187] The results showed that the 40% compound (I)·bromothalonil wettable powder (20:20), 28% compound (I)·cyenopyrac suspension concentrate (24:4), and 30% compound (I)·prochloraz suspension concentrate (20:10) had good control effects on Colletotrichum capsici. Seven days after the last application, their control effects were 85.39%, 84.55%, and 82.26% respectively; there were significant differences between the compound preparations and other single agents.

[0188] Table 6 Results of field efficacy trials (7 days after the last application)

[0189]

[0190] Note: The data in the table are the average values of 4 replicates; lowercase letters indicate significant differences between treatments (p < 0.05)

[0191] During the test, no phytotoxicity or effects on crop growth and development were recorded, indicating that the test agents were safe for the crops within the recommended dosage. No effects on non-target organisms were found within the dosage range of the agents. No extreme weather conditions such as heavy rainfall or strong winds were recorded during the test period.

[0192] The results of the field efficacy plot trials showed that the compound (I) and its mixtures with bromothalonil, cyenopyrac, and prochloraz showed high control effects in the control of Colletotrichum capsici. They not only reduced the disease fruit rate and disease index, had significant control effects, and had little impact on the yield, but also showed obvious yield-increasing effects. In addition, according to the observations after application, the peppers in each treatment area grew normally without phytotoxicity, indicating that each agent was safe for pepper growth at the dosage used in this test.

[0193] Through indoor toxicity determination and field trials, the composition obtained by compounding the compound represented by formula (I) of the present invention with any one of bromothalonil, cyprodinil, and prochloraz shows a very good control effect against Colletotrichum capsici. The bactericidal composition or its preparation obtained by the compounding of the present invention has a significant control effect, and has the characteristics of high efficiency, broad spectrum, low residue, long-lasting efficacy, strong systemic absorption, etc.; and no phytotoxicity to crops was found in the tests, indicating that under the condition of improved bactericidal synergistic effect of the obtained bactericidal composition or preparation, the production cost and use cost can be reduced, and it is safe for crops.

[0194] Although the present invention has been described in detail above with general descriptions and specific embodiments, based on the present invention, some modifications or improvements can be made, which are obvious to those skilled in the art. Therefore, these modifications or improvements made without departing from the spirit of the present invention all fall within the scope of protection required by the present invention.

Claims

1. A bactericidal composition, characterized in that: Comprising active ingredient A and active ingredient B, wherein the active ingredient A is a compound represented by formula (I): The active ingredient B is any one of bromothalonil, phenamacril, and prochloraz.

2. The bactericidal composition according to claim 1, characterized in that, The mass ratio of the active ingredient A to the active ingredient B is 1:36 to 34:

1.

3. The bactericidal composition according to claim 1, wherein The mass ratio of the compound of formula (I) to cymoxanil is 1:32 to 32:1; The mass ratio of the compound of formula (I) to cyenopyrafen is 1:30 to 25:1; The mass ratio of the compound of formula (I) to prochloraz is 1:36 to 34:

1.

4. The bactericidal composition according to claim 3, characterized in that, The mass ratio of the compound of formula (I) to cymoxanil is 1:24 to 32:1; The mass ratio of the compound of formula (I) to cyenopyrafen is 1:25 to 25:1; The mass ratio of the compound of formula (I) to prochloraz is 1:25 to 20:

1.

5. The bactericidal composition according to claim 1, wherein Based on the total weight of the bactericidal composition being 100 wt%, the sum of the contents of active ingredient A and active ingredient B in the bactericidal composition is 1 to 90 wt%; Preferably, based on the total weight of the bactericidal composition being 100 wt%, the sum of the contents of active ingredient A and active ingredient B in the bactericidal composition is 5 to 80 wt%.

6. The bactericidal composition according to claim 1, wherein The bactericidal composition further comprises agriculturally acceptable auxiliary ingredients in addition to the active ingredients, and the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreezing agents, defoaming agents, solvents, preservatives, stabilizers, synergists, binders or carriers.

7. The bactericidal composition according to claim 6, characterized in that, The dosage form of the bactericidal composition is selected from solid preparations and / or liquid preparations.

8. The bactericidal composition according to claim 7, wherein The bactericidal composition can be prepared into a pesticide-acceptable preparation dosage form, and the preparation dosage form is microemulsion, water emulsion, suspension, dispersible oil suspension, solution, emulsifiable concentrate, suspo-emulsion, microcapsule suspension, water dispersible granule, wettable powder, granule, seed treatment suspension, seed treatment dry powder; Preferably, the preparation dosage form is microemulsion, emulsifiable concentrate, suspension, water emulsion, water dispersible granule, wettable powder, seed treatment suspension.

9. Use of the bactericidal composition according to any one of claims 1-8 in controlling plant diseases.

10. The application according to claim 9, wherein The plant diseases are plant diseases caused by fungi or bacteria; Preferably, the plant diseases are plant diseases caused by fungi; More preferably, the plant diseases caused by fungi are pepper anthracnose.

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