Bacteriostatic agent and application thereof in controlling fruit diseases
By using antibacterial agents prepared by 5-iodoisoquinoline (DS), the problem of green prevention and control of post-harvest crown rot was solved, effective control of Fusarium sprouted, improved the quality and storage period of fruits, and promoted the sustainable development of agriculture.
Patent Information
- Application Number
- CN202510502175.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-21
- Publication Date
- 2025-07-11
AI Technical Summary
The prior art lacks effective green prevention and treatment methods for post-harvest crown rot in bananas. The use of chemical agents is not conducive to the health of the eater, and the emergence of Fusarium is difficult to control.
5-iodoisoquinoline (DS) is used as an active ingredient for antibacterial agents, combined with neutral or weak acidic solvents such as water, which may contain surfactants, thickeners, etc., to prepare antibacterial agents, and are applied to the fruit surface by soaking, spraying or applying them to control the growth of Fusarium sprouts.
Effectively inhibit the growth of Fusarium sprouts, maintain fruit quality, extend storage period, improve crop yield, and promote green and sustainable development of agriculture.
Smart Images
Figure CN120283770A_ABST
Abstract
Description
Technical Field
[0001] The present invention belongs to the field of green ecological control of plant diseases, and particularly relates to a novel bacteriostatic agent and the application of the bacteriostatic agent in controlling Fusarium proliferatum diseases. Background Art
[0002] Crown rot is one of the important fungal diseases of bananas after harvest, which leads to a significant decline in the nutrition and quality of bananas after harvest, causing major economic losses. Due to the lack of effective control methods, banana crown rot brings great difficulties to the post-harvest storage and transportation of bananas in China, and has gradually become one of the main diseases of bananas after harvest. Fusarium proliferatum is one of the main pathogenic bacteria causing banana crown rot after harvest. At present, chemical agents are mainly used for prevention and control, which have a certain impact on fruits and are not conducive to the health of consumers. Therefore, screening for safe and effective green fungicides for humans to control the growth and infection of Fusarium proliferatum is of great significance for alleviating the further spread of banana crown rot after harvest, maintaining the quality of bananas and extending the storage period.
[0003] Isoquinoline derivatives are some commonly used drugs in medicine. For example, berberine is a naturally occurring isoquinoline alkaloid and is widely used in traditional medicine. 5-Iodoisoquinoline (DS) is an iodine-containing isoquinoline organic compound, which has neuroprotective and anti-inflammatory properties in nerve tissues and is used as a drug in neuroscience. However, the role and related applications of 5-iodoisoquinoline in controlling post-harvest diseases of fruits have not been reported yet. Summary of the Invention
[0004] Based on the problems existing in the background art, the object of the present invention is to provide an effective and safe green fungicide and its application, specifically, its application in controlling fruit diseases caused by Fusarium proliferatum.
[0005] On the one hand, the present invention provides a bacteriostatic agent, which is characterized in that the bacteriostatic agent contains 5-iodoisoquinoline (DS) and a solvent. The active ingredient of the bacteriostatic agent is 5-iodoisoquinoline (DS). The bacteriostatic agent can inhibit the growth of Fusarium proliferatum.
[0006] In a preferred embodiment, the solvent is a neutral or weakly acidic liquid.
[0007] Furthermore, the neutral or weakly acidic liquid can be water.
[0008] In another preferred embodiment, the bacteriostatic agent of the present invention further contains a surfactant for enhancing the adhesion of the liquid on the plant surface.
[0009] In another preferred embodiment, the bacteriostatic agent of the present invention may further contain at least one of a thickener, a stabilizer, a co-solvent, a wetting agent, etc.
[0010] Furthermore, the concentration of 5-iodoisoquinoline (DS) in the bacteriostatic agent is 0.05 to 2 mM.
[0011] In a preferred embodiment, the concentration of 5-iodoisoquinoline (DS) in the bacteriostatic agent is 0.1 to 1 mM, and more preferably 0.1 to 0.2 mM.
[0012] Furthermore, the bacteriostatic agent is a fruit preservative.
[0013] Furthermore, the bacteriostatic agent has the effect of inhibiting the growth of Fusarium proliferatum.
[0014] On the other hand, the present invention provides the use of 5-iodoisoquinoline (DS) in the preparation of a bacteriostatic agent.
[0015] In a preferred embodiment, the bacteriostatic agent of the present invention contains 5-iodoisoquinoline (DS) and a solvent.
[0016] In a preferred embodiment, the solvent is a neutral or weakly acidic liquid. Furthermore, the neutral or weakly acidic liquid may be water.
[0017] In another preferred embodiment, the bacteriostatic agent of the present invention further contains a surfactant for enhancing the adhesion of the liquid to the plant surface. Furthermore, the bacteriostatic agent of the present invention may further contain at least one of a thickener, a stabilizer, a co-solvent, a wetting agent, etc.
[0018] Furthermore, the concentration of 5-iodoisoquinoline (DS) in the bacteriostatic agent is 0.05 to 2 mM.
[0019] In a preferred embodiment, the concentration of 5-iodoisoquinoline (DS) in the bacteriostatic agent is 0.1 to 1 mM, and more preferably 0.1 to 0.2 mM.
[0020] On the other hand, the present invention provides the use of the above bacteriostatic agent in fruit preservation.
[0021] In a preferred embodiment, the active ingredient of the bacteriostatic agent is 5-iodoisoquinoline. In a preferred embodiment, the fruit preservation is achieved by inhibiting pathogenic bacteria with a bacteriostatic agent containing 5-iodoisoquinoline (DS). More specifically, the bacteriostatic agent can inhibit the growth of Fusarium proliferatum.
[0022] Furthermore, the concentration of 5-iodoisoquinoline (DS) in the bacteriostatic agent is 0.05 to 2 mM.
[0023] In a preferred embodiment, the bacteriostatic agent contains 5-iodoisoquinoline (DS) at a concentration of 0.1 - 1 mM, more preferably 0.1 - 0.2 mM.
[0024] Furthermore, the bacteriostatic agent can achieve the bacteriostatic effect by soaking, spraying or smearing.
[0025] Furthermore, the objects for soaking, spraying or smearing can be fruits or the packaging materials for fruits.
[0026] On the other hand, the present invention also provides the use of 5-iodoisoquinoline (DS) in the preparation of a preservative.
[0027] In a preferred embodiment, the preservative contains 5-iodoisoquinoline (DS) and a solvent.
[0028] In a preferred embodiment, the solvent is a neutral or weakly acidic liquid. Further, the neutral or weakly acidic liquid can be water.
[0029] In another preferred embodiment, the preservative of the present invention further contains a surfactant for enhancing the adhesion of the liquid on the surface of plants. Further, the preservative of the present invention can further contain at least one of a thickener, a stabilizer, a co-solvent, a wetting agent, etc. Further, the preservative contains 5-iodoisoquinoline (DS) at a concentration of 0.05 - 2 mM, preferably 0.1 - 1 mM, more preferably 0.1 - 0.2 mM.
[0030] On the other hand, the present invention provides the use of the above 5-iodoisoquinoline (DS) in the screening of bacteriostatic agents.
[0031] Furthermore, the use of 5-iodoisoquinoline (DS) in the screening of bacteriostatic agents is to use 5-iodoisoquinoline (DS) as a positive control.
[0032] Furthermore, the use of 5-iodoisoquinoline (DS) in the screening of bacteriostatic agents includes the following steps: 1) Culturing microorganisms; 2) Treating the microorganisms cultured in step 1) with a drug containing 5-iodoisoquinoline (DS) and a candidate drug; 3) Determining whether the candidate drug has a bacteriostatic effect by comparing the effects of the drug containing 5-iodoisoquinoline (DS) and the candidate drug on the treatment of microorganisms.
[0033] Preferably, the microorganism or bacterium is Fusarium proliferatum.
[0034] Compared with the prior art, the present invention has the following advantages: (1) The present invention provides a new use of 5-iodoisoquinoline (DS), that is, for the preparation of a safe and effective green bactericide for humans.
[0035] (2) The present invention for the first time provides the application of 5-iodoisoquinoline in controlling fruit diseases. By applying 5-iodoisoquinoline, plant diseases caused by Fusarium proliferatum can be effectively controlled; the fruit quality can be maintained, the shelf life can be extended, and the crop yield can also be increased through field application, promoting the green and sustainable development of agriculture.
[0036] (3) The present invention also for the first time provides the use of 5-iodoisoquinoline as a positive control for screening bacteriostatic agents. Since 5-iodoisoquinoline has obvious bacteriostatic effect, using it as a positive control can effectively screen more bacteriostatic agents. Description of the Drawings
[0037] The following combines the drawings and specific embodiments to detail the solution of the present invention and its beneficial effects.
[0038] Figure 1 . Effects of 5-iodoisoquinoline (DS) treatment on the growth of Fusarium proliferatum on PDA plates; (A), phenotypes of Fusarium proliferatum treated with different concentrations of DS; (B), histogram of the colony diameter of Fusarium proliferatum when treated with 0.1 mM DS, p<0.05, n = 3.
[0039] Figure 2 . Growth of Escherichia coli treated with different concentrations of DS on solid medium.
[0040] Figure 3 . Growth of Saccharomyces cerevisiae treated with different concentrations of DS on solid medium.
[0041] Figure 4 . Effects of DS treatment (0.1 mM) on the infection of bananas by Fusarium proliferatum; (A), phenotypes of infected bananas; (B), histogram of the plaque diameter, p<0.05, n = 16. Detailed Embodiments
[0042] The technical solutions in the embodiments of the present invention are clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts belong to the protection scope of the present invention.
[0043] Unless otherwise defined, all technical and scientific terms used in this application have the same meaning as commonly understood by those of ordinary skill in the technical field to which this application belongs.
[0044] Example 1 Bacteriostatic Effect of 5-Iodoisoquinoline (DS) in Medium Based on the morphological characteristics of different microorganisms, when culturing microorganisms on plates, in this embodiment, the unicellular microorganisms yeast and Escherichia coli are cultured by the plate coating method, and the filamentous fungus Fusarium spp. is cultured by the spot planting method, that is, inoculating a spore suspension in the center of the plate for culture.
[0045] 1. Effect of 5-iodoisoquinoline (DS) treatment on the growth of Fusarium solani Fusarium spp. was cultured on PDA plates at 28°C for 7 days. The spores were washed with sterile water and filtered through gauze. The filtrate was collected and the spore concentration was adjusted to 1 × 10 6 spores / ml. 10 μl of the above spores were dropped in the middle of PDA medium containing 0 mM DS and PDA medium containing 0.05 mM, 0.1 mM and 0.2 mM DS, respectively. After 7 days of culture, the effect of DS on the growth of Fusarium was compared. Figure 1 As shown in A, DS treatment could inhibit the growth of Fusarium solani starting from 0.05 mM.
[0046] We further selected the minimum inhibitory concentration of 0.1 mM, conducted three repeated tests, and counted the colony diameters. The colony diameters of the treatment group were significantly smaller than those of the control group CK (p<0.05, Figure 1 B). This indicates that DS can effectively control the growth of Fusarium spp.
[0047] 2. Effect of 5-iodoisoquinoline (DS) treatment on the growth of Escherichia coli The E. coli strain was streaked onto a YLB solid plate for activation, and a single colony was picked and inoculated into LB liquid medium. After shaking and culturing at 37°C and 200 rpm for 16 h, the OD was measured. 600 . Dilute with ddH2O to OD 600 0.0001, take 0.1 ml of the diluted bacterial solution and spread it evenly on LB solid medium containing 0 mM, 0.05 mM, 0.1 mM and 0.2 mM DS, culture at 37℃ for 16h, and take photos. Figure 2 As shown in the figure, under solid culture conditions, the growth of E. coli under different concentrations of DS is basically the same, indicating that DS has no inhibitory effect on E. coli.
[0048] 3. Effect of 5-iodoisoquinoline (DS) treatment on the growth of Saccharomyces cerevisiae The Saccharomyces cerevisiae strain (BY4741) was streaked onto a YPDA solid plate for activation, and a single colony was picked and inoculated into YPD liquid medium. After shaking and culturing at 30°C and 200 rpm for 16 h, the OD was measured. 600 . Dilute with ddH2O to OD 600was 0.0001. 0.1 ml of the diluted bacterial solution was taken and evenly spread on YPDA solid medium containing 0 mM, 0.05 mM, 0.1 mM and 0.2 mM DS, and cultured at a constant temperature of 37 °C for 16 h, and photographed and recorded. By comparing the growth of Saccharomyces cerevisiae, it was found that compared with the control, DS at different concentrations had no obvious effect on the growth of Saccharomyces cerevisiae (see Figure 3 ). It shows that DS has no inhibitory effect on Saccharomyces cerevisiae.
[0049] After in-depth research, the inventors found that 5-iodoisoquinoline has no inhibitory effect on other bacteria except Fusarium proliferatum. The above results indicate that DS has no inhibitory effect on Escherichia coli and yeast, but has a significant inhibitory effect on Fusarium proliferatum, indicating that DS has a certain selectivity in the inhibition of microorganisms.
[0050] Example 2 Antibacterial effect of 5-iodoisoquinoline (DS) on fruits Banana (Musa acuminata AAA group, cv. Cavendish) fruits harvested on a sunny day, eight-ripe, without mechanical damage and pests and diseases were selected. The banana fruits were randomly divided into two groups, and the control group and the experimental group were respectively immersed in water and a DS solution with a concentration of 0.1 mM for 5 min, and the immersed banana fruits were dried. The Fusarium proliferatum was inoculated by the acupuncture method: 10 μL of Fusarium proliferatum spores (1×10 6 spores / ml) were dropped onto the acupuncture sites of the banana fruits. All banana fruits were stored in an incubator at 25 °C and a relative humidity of 85%, and the disease incidence of the bananas was observed. Four days after inoculation, it was first observed with the naked eye that the banana peel treated with DS was less diseased ( Figure 4 A), and the diameter of the bacterial plaque on the banana peel treated with DS was significantly smaller than that of the control group CK (p<0.05, Figure 4 B). It shows that the treatment with DS can effectively control the occurrence of crown rot.
[0051] Example 3 Antibacterial agent containing 5-iodoisoquinoline (DS) When preparing an antibacterial agent containing 5-iodoisoquinoline, antibacterial agents with corresponding components can be prepared according to the following two usage scenarios.
[0052] 1. An antibacterial agent for soaking or spraying can effectively inhibit fruit diseases caused by Fusarium proliferatum.
[0053] 2. An antibacterial agent for spraying in the forest can effectively inhibit plant diseases caused by Fusarium proliferatum.
[0054] The foregoing description of the disclosed embodiments enables those skilled in the art to implement or use the present invention. Various modifications to the above-described embodiments will be apparent to those skilled in the art, and the general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims
1. An antibacterial agent, characterized in that, The bacteriostatic agent contains 5-iodoisoquinoline and a solvent.
2. The bacteriostatic agent according to claim 1, wherein The bacteriostatic agent further contains at least one of a surfactant, a thickener, a cosolvent, a wetting agent, and a stabilizer.
3. The use of 5-iodoisoquinoline in the preparation of a bacteriostatic agent.
4. The application according to claim 3, wherein The bacteriostatic agent contains 5-iodoisoquinoline and a solvent.
5. The use of 5-iodoisoquinoline in the preparation of a preservative.
6. The use of a bacteriostatic agent containing 5-iodoisoquinoline in fruit preservation.
7. The application according to claim 6, characterized in that, The fruit preservation is achieved by inhibiting the growth of pathogenic bacteria with 5-iodoisoquinoline.
8. The application according to claim 6, characterized in that The concentration of 5-iodoisoquinoline contained in the bacteriostatic agent is 0.05 - 2 mM.
9. The application according to claim 6, wherein The bacteriostatic effect can be achieved by soaking, spraying, or smearing the bacteriostatic agent. 10.5-Iodoisoquinoline's application in the screening of bacteriostatic agents, characterized in that, The 5-iodoisoquinoline is used as a positive control in the screening of bacteriostatic agents.