Mango embryo promoting agent and embryo promoting method

Abstract

The invention provides a mango embryo-promoting agent and an embryo-promoting method, and belongs to the technical field of plant planting. The mango embryo-promoting agent comprises an inflorescence development stage embryo-promoting solution I; the formula of the embryo promoting solution I in the inflorescence development period comprises an MS culture solution, AVG, GA3, imidacloprid, spinetoram, 6-BA and KH2PO4; according to the mango embryo promoting method, a mango embryo promoting agent is applied in the mango planting process so as to promote embryo development of mangoes. According to the mango embryo promoting agent and the embryo promoting method disclosed by the invention, the embryo-free fruit proportion of mangoes can be reduced to about 80%, and the reduction amplitude is about 10%.

Description

Technical Field

[0001] The present invention belongs to the technical field of plant cultivation, and particularly relates to a mango embryo-promoting agent and an embryo-promoting method. Background Art

[0002] Mangoes are rich in carbohydrates, proteins, fatty acids, vitamins, organic acids, dietary fiber, polyphenols, carotenoids, etc. Regular consumption can prevent obesity, diabetes, hypertension, and cancer, and has high economic value and nutritional value. China is the second largest mango producer in the world. In 2020, the national mango planting area was 5.13 million mu, the output was 2.67 million tons, and the output value of fresh fruits was 17 billion yuan. There are 1.34 million households and 5.34 million people engaged in mango and related industries across the country.

[0003] The current main mango cultivars in China, such as "Tainong No. 1", "Jinhuang", and "Kate", all have parthenocarpic fruits and seeded fruits. Among them, the single fruit weight of seeded fruits is about 3 times that of parthenocarpic fruits. Under natural conditions, parthenocarpic fruits account for about 90% on each tree. The standard for commercial mango fruits is that the single fruit weight is above 100 grams. In production, about 70% of the single fruit weights of parthenocarpic fruits do not meet this standard. That is to say, about 1.5 million tons of mangoes do not meet the standard of commercial fruits every year and cannot be sold at the price of commercial fruits. The annual loss due to parthenocarpic fruits is about 9.5 billion yuan. If all fruits are seeded fruits, not only can the annual loss of about 9.5 billion yuan be reduced, but also the annual output can be increased to 5.721 million tons. Without increasing the existing land use, the output increases by 1.14 times, the output value increases by 28.8 billion yuan, and the output value increases by 1.69 times. Therefore, it is necessary to develop a mango embryo-promoting agent and an embryo-promoting method to reduce the proportion of parthenocarpic fruits. Summary of the Invention

[0004] In view of the above problems, the present invention provides a mango embryo-promoting agent and an embryo-promoting method, which can significantly reduce the incidence of parthenocarpic fruits in mangoes, increase the yield of mangoes per tree, and increase the income of fruit farmers.

[0005] To achieve the above object, the technical solution adopted by the present invention is as follows: A mango embryo-promoting agent, wherein the mango embryo-promoting agent includes an embryo-promoting solution I during the inflorescence development period; The formula of the embryo-promoting solution I during the inflorescence development period includes: MS culture medium, Aminoethoxyvinylglycine (AVG), Gibberellin A3 (GA3), Imidacloprid, Spinetoram, 6-Benzylaminopurine (6-BA), and KH2PO4.

[0006] Furthermore, the embryo-promoting solution I during the inflorescence development stage uses MS culture medium as a solvent, wherein the concentration of aminoethoxyvinylglycine is 10 - 130 ppm, the concentration of gibberellin A3 is 20 - 300 ppm, the concentration of imidacloprid is 1 - 10 g / L, the concentration of spinetoram is 10 - 100 g / L, the concentration of 6-benzylaminopurine is 10 - 200 mg / L, and the concentration of KH2PO4 is 200 - 2000 mg / L.

[0007] Furthermore, the mango embryo-promoting agent further includes the embryo-promoting solution II during the full-bloom stage; The formulation of the embryo-promoting solution II during the full-bloom stage includes: MS culture medium, aminoethoxyvinylglycine (Aminoethoxyvinylglycine, AVG), imidacloprid, spinetoram, gibberellin, and compound amino acid trace element chelate.

[0008] Furthermore, the gibberellin used in the embryo-promoting solution II during the full-bloom stage is gibberellin A3, gibberellin A4, or gibberellin A7; The embryo-promoting solution II during the full-bloom stage uses MS culture medium as a solvent, wherein the concentration of aminoethoxyvinylglycine is 10 - 130 ppm, the concentration of imidacloprid is 1 - 10 g / L, the concentration of spinetoram is 10 - 100 g / L, the concentration of gibberellin is 10 - 50 mg / L, and the concentration of compound amino acid trace element chelate is 500 - 2500 mg / L.

[0009] Furthermore, the mango embryo-promoting agent further includes the embryo-promoting solution III during the fruit expansion stage; The formulation of the embryo-promoting solution III during the fruit expansion stage includes: MS culture medium, aminoethoxyvinylglycine (Aminoethoxyvinylglycine, AVG), gibberellin A3 (GA3), imidacloprid, spinetoram, indoleacetic acid (IAA), and urea.

[0010] Furthermore, the embryo-promoting solution III during the fruit expansion stage uses MS culture medium as a solvent, wherein the concentration of aminoethoxyvinylglycine is 10 - 130 ppm, the concentration of gibberellin A3 is 20 - 300 ppm, the concentration of imidacloprid is 1 - 10 g / L, the concentration of spinetoram is 10 - 100 g / L, the concentration of indoleacetic acid is 20 - 100 mg / L, and the concentration of urea is 1000 - 5000 mg / L.

[0011] Furthermore, the mango embryo-promoting agent further includes the embryo-promoting solution IV after the expansion stage; The formula of the embryo-promoting solution IV after the swelling period includes: MS culture medium, Aminoethoxyvinylglycine (AVG), Gibberellin A3 (GA3), Imidacloprid, Spinetoram, Indole-3-acetic acid (IAA), and compound amino acid trace element chelate.

[0012] Furthermore, the embryo-promoting solution IV after the swelling period uses MS culture medium as the solvent. Among them, the concentration of Aminoethoxyvinylglycine is 10 - 130 ppm, the concentration of Gibberellin A is 20 - 300 ppm, the concentration of Imidacloprid is 1 - 10 g / L, the concentration of Spinetoram is 10 - 100 g / L, the concentration of Indole-3-acetic acid is 20 - 100 mg / L, and the concentration of compound amino acid trace element chelate is 500 - 3000 mg / L.

[0013] A method for promoting embryo development of mangoes, which is to apply the above-mentioned mango embryo-promoting agent during the mango planting process to promote the embryo development of mangoes.

[0014] Furthermore, the mango embryo-promoting agent includes the embryo-promoting solution I during the inflorescence development period, the embryo-promoting solution II during the full-bloom period, the embryo-promoting solution III during the fruit swelling period, and the embryo-promoting solution IV after the swelling period; During the mango planting process, apply the embryo-promoting solution I during the initial stage of inflorescence development; Apply the embryo-promoting solution II during the full-bloom period; Apply the embryo-promoting solution III during the fruit swelling period; Apply the embryo-promoting solution IV 15 days after the swelling period; The embryo-promoting solution I during the inflorescence development period uses MS culture medium as the solvent. Among them, the concentration of Aminoethoxyvinylglycine is 10 - 130 ppm, the concentration of Gibberellin A3 is 20 - 300 ppm, the concentration of Imidacloprid is 1 - 10 g / L, the concentration of Spinetoram is 10 - 100 g / L, the concentration of 6-Benzylaminopurine is 10 - 200 mg / L, and the concentration of KH2PO4 is 200 - 2000 mg / L; The embryo-promoting solution II during the full-bloom period uses MS culture medium as the solvent. Among them, the concentration of Aminoethoxyvinylglycine is 10 - 130 ppm, the concentration of Imidacloprid is 1 - 10 g / L, the concentration of Spinetoram is 10 - 100 g / L, the concentration of Gibberellin is 10 - 50 mg / L, and the concentration of compound amino acid trace element chelate is 500 - 2500 mg / L; The Gibberellin used in the embryo-promoting solution II during the full-bloom period is Gibberellin A3, Gibberellin A4, or Gibberellin A7; The embryo-promoting solution III during the fruit swelling period uses MS culture solution as the solvent. Among them, the concentration of aminoethoxyvinylglycine is 10 - 130 ppm, the concentration of gibberellin A3 is 20 - 300 ppm, the concentration of imidacloprid is 1 - 10 g / L, the concentration of spinetoram is 10 - 100 g / L, the concentration of indoleacetic acid is 20 - 100 mg / L, and the concentration of urea is 1000 - 5000 mg / L; The embryo-promoting solution IV after the swelling period uses MS culture solution as the solvent. Among them, the concentration of aminoethoxyvinylglycine is 10 - 130 ppm, the concentration of gibberellin A is 20 - 300 ppm, the concentration of imidacloprid is 1 - 10 g / L, the concentration of spinetoram is 10 - 100 g / L, the concentration of indoleacetic acid is 20 - 100 mg / L, and the concentration of compound amino acid trace element chelate is 500 - 3000 mg / L.

[0015] The beneficial effects of a mango embryo-promoting agent and an embryo-promoting method of the present invention are as follows: Since mango flowers are divided into male flowers and bisexual flowers, about 5 days after fertilization, the first large-scale drop of mango flowers occurs, and about 25 days after fertilization, the second large-scale flower drop occurs. The present invention uses AVG and GA3 in combination to protect mango flowers and reduce the proportion of mango flower drops; among them, because ethylene can promote the formation of the abscission layer of flower stalks and fruit stalks, promoting flower and fruit drop, and AVG is an ethylene inhibitor that can block the synthesis of the ethylene precursor ACC, reducing the ethylene concentration and the proportion of mango flower drops; at the same time, mango flower and fruit abscission occur in the abscission layer. The mango abscission layer has only two layers of densely arranged cells, and the xylem and phloem are broken. GA3 can promote the elongation of the cells on both sides of the abscission layer, increase the connection of the cells on both sides of the abscission layer, reduce the separation of the abscission layer, and reduce the proportion of mango flower drops; After the mango flower is fertilized, embryo abortion during the development of the young fruit is the main reason for the formation of mango seedless fruits; the reason for embryo abortion lies in insufficient nutrient supply and lack of auxin; the mango embryo-promoting agent formula of the present invention contains MS culture solution, compound amino acid trace element chelate and IAA, which can supplement the nutrient elements and auxin consumed during the development of mango young fruits and reduce the proportion of embryo abortion; The mango pulp comes from the ovary wall, and the pericarp comes from the ovary epidermis; inside the pulp is the seed, which consists of two parts: endosperm and embryo; the seed comes from double fertilization, where the sperm combines with two polar nuclei to form the triploid endosperm, and the sperm combines with the egg to form the embryo; the essence of the mango seedless fruit is the abortion of mango seed development; the reasons for the abortion of mango seed development are various, possibly due to fertilization failure, insufficient nutrient supply after fertilization, insufficient auxin content, etc.; the mango embryo promoter of the present invention can increase the nutrition of mango fruits and reduce the proportion of seedless fruits by supplementing MS culture solution and complex amino acid trace element chelates; by supplementing IAA, it can supplement the auxin content of young mango fruits, promote embryo development, and reduce the proportion of seedless fruits. During the flowering and young fruit development periods of mangoes, thrips occur frequently, and most of the flowers and young fruits bitten by thrips fall off; the formula of the mango embryo promoter of the present invention contains imidacloprid and spinetoram, which can effectively kill thrips and reduce the abscission of flowers and young fruits caused by thrips biting. The mango embryo promoter and embryo promotion method of the present invention can reduce the proportion of mango seedless fruits to about 80%, with a reduction amplitude of about 10%. Description of the Drawings

[0016] Figure 1 It is the hanging application diagram of "Tainong No. 1" mango in Example 1 of the present invention; Figure 2 It is the hanging application diagram of "Tainong No. 1" mango in Example 1 of the present invention; Figure 3 It is the comparison diagram of seeded and seedless fruits of "Guifei" mango in Example 2 of the present invention; Figure 4 It is the statistical chart of seeded and seedless fruits of "Guifei" mango in Example 2 of the present invention; Figure 5 It is the inspection result of embryo development of "Guifei" mango in Example 2 of the present invention; Figure 6 It is the inspection result of embryo development of "Guifei" mango in Example 2 of the present invention; Figure 7 It is the hanging application diagram of "Jinhuang" mango in Example 3 of the present invention; Figure 8 It is the comparison diagram of seeded and seedless fruits of "Jinhuang" mango in Example 3 of the present invention; Figure 9 It is the statistical chart of seeded and seedless fruits of "Jinhuang" mango in Example 3 of the present invention. Detailed Description of the Invention

[0017] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Many specific details are set forth in the following description in order to fully understand the present invention, but the present invention can also be implemented in other ways different from those described herein. Those skilled in the art can make similar promotions without departing from the connotation of the present invention. Therefore, the present invention is not limited by the specific embodiments disclosed below.

[0018] Example 1 A method for promoting embryo development of mango This example is a method for promoting embryo development of mango, and the specific process includes the following steps: I. Preparation of mango embryo-promoting agent In this example, the mango embryo-promoting agent includes embryo-promoting solution I during the inflorescence development period, embryo-promoting solution II during the full-bloom period, embryo-promoting solution III during the fruit expansion period, and embryo-promoting solution IV after the expansion period. Specifically: Take MS culture medium and add aminoethoxyvinylglycine (AVG), gibberellin A3 (GA3), imidacloprid, spinetoram, 6-benzylaminopurine (6-BA), and KH2PO4, dissolve them to prepare three different concentrations of embryo-promoting solution I during the inflorescence development period, namely low, medium, and high concentrations. Among them, the concentrations of AVG in the low, medium, and high concentrations of embryo-promoting solution I during the inflorescence development period are 10, 62.5, and 125 ppm in sequence, the concentrations of GA3 are 45, 135, and 270 ppm in sequence, the concentrations of imidacloprid are 1, 2, and 3 g / L in sequence, the concentrations of spinetoram are 30, 60, and 90 g / L in sequence, the concentrations of 6-BA are 60, 90, and 120 mg / L in sequence, and the concentrations of KH2PO4 are 1000, 1500, and 2000 mg / L in sequence.

[0019] Take MS culture medium and add aminoethoxyvinylglycine (AVG), imidacloprid, spinetoram, gibberellin (gibberellin A3, gibberellin A4, or gibberellin A7 can be used, and gibberellin A3, that is, GA3, is used in this example), and complex amino acid trace element chelate (purchased from Shanghai Yuanye Bio-Technology Co., Ltd.), dissolve them to prepare three different concentrations of embryo-promoting solution II during the full-bloom period, namely low, medium, and high concentrations. Among them, the concentrations of AVG in the low, medium, and high concentrations of embryo-promoting solution II during the full-bloom period are 10, 62.5, and 125 ppm in sequence, the concentrations of imidacloprid are 1, 2, and 3 g / L in sequence, the concentrations of spinetoram are 30, 60, and 90 g / L in sequence, the concentrations of GA3 are 20, 30, and 40 mg / L in sequence, and the concentrations of complex amino acid trace element chelate are 1200, 1800, and 2400 mg / L in sequence.

[0020] Take MS culture medium and add Aminoethoxyvinylglycine (AVG), Gibberellin A3 (GA3), Imidacloprid, Spinetoram, Indole-3-acetic acid (IAA) and Urea, dissolve them to prepare three different concentrations of embryo-promoting solution III during the fruit expansion period, namely low, medium and high concentrations. Among them, the concentrations of AVG in the three different concentrations of embryo-promoting solution III during the fruit expansion period are 10, 62.5, 125 ppm in sequence, the concentrations of GA3 are 45, 135, 270 ppm in sequence, the concentrations of Imidacloprid are 1, 2, 3 g / L in sequence, the concentrations of Spinetoram are 30, 60, 90 g / L in sequence, the concentrations of IAA are 40, 60, 80 mg / L in sequence, and the concentrations of Urea are 3000, 3500, 4000 mg / L in sequence.

[0021] Take MS culture medium and add Aminoethoxyvinylglycine (AVG), Gibberellin A3 (GA3), Imidacloprid, Spinetoram, Indole-3-acetic acid (IAA) and compound amino acid trace element chelate (purchased from Shanghai Yuanye Bio-Technology Co., Ltd.), dissolve them to prepare three different concentrations of embryo-promoting solution IV after the expansion period, namely low, medium and high concentrations. Among them, the concentrations of AVG in the three different concentrations of embryo-promoting solution IV after the expansion period are 10, 62.5, 125 ppm in sequence, the concentrations of GA3 are 45, 135, 270 ppm in sequence, the concentrations of Imidacloprid are 1, 2, 3 g / L in sequence, the concentrations of Spinetoram are 30, 60, 90 g / L in sequence, the concentrations of IAA are 40, 60, 80 mg / L in sequence, and the concentrations of compound amino acid trace element chelate are 1200, 1800, 2400 mg / L in sequence.

[0022] II. Embryo-promoting method As Figure 1 and Figure 2 shown, during the cultivation of "Tainong No. 1" mango, at the initial stage of inflorescence development, 500 mL of embryo-promoting solution I during the inflorescence development period is drip-fed to each tree, adjust the drip rate, finish dripping in two days, and then continue to drip-feed the second bag of embryo-promoting solution I during the inflorescence development period at the same drip rate until entering the full bloom stage. The embryo-promoting solution I for drip-feeding can be any one of the three concentrations of embryo-promoting solution I during the inflorescence development period, namely low, medium and high concentrations.

[0023] When entering the full bloom stage, stop drip-feeding the embryo-promoting solution I during the inflorescence development period, and drip-feed 500 mL of embryo-promoting solution II during the full bloom stage to each tree, adjust the drip rate, finish dripping in two days, and then continue to drip-feed the second bag of embryo-promoting solution II during the full bloom stage at the same drip rate until entering the fruit expansion period. The embryo-promoting solution II for drip-feeding can be any one of the three concentrations of embryo-promoting solution II during the full bloom stage, namely low, medium and high concentrations.

[0024] When entering the fruit swelling period, stop hanging and applying the embryo-promoting solution II during the full-bloom period. Apply 500 mL of the embryo-promoting solution III during the fruit swelling period to each tree, adjust the dripping speed, and finish dripping in two days. After dripping, continue to hang and apply the second bag of the embryo-promoting solution III during the fruit swelling period at the same dripping speed until the end of the fruit swelling period. The embryo-promoting solution III applied during the fruit swelling period can be any one of the low, medium, and high concentrations of the embryo-promoting solution III during the fruit swelling period.

[0025] After the end of the fruit swelling period, stop hanging and applying the embryo-promoting solution III during the fruit swelling period, and continue to grow for 14 days, that is, 15 days after the swelling period. Apply 500 mL of the embryo-promoting solution IV after the swelling period to each tree, adjust the dripping speed, and finish dripping in two days. After dripping, continue to hang and apply the second bag of the embryo-promoting solution IV after the swelling period at the same dripping speed until the mangoes are ripe. The embryo-promoting solution IV applied after the swelling period can be any one of the low, medium, and high concentrations of the embryo-promoting solution IV after the swelling period.

[0026] After the mangoes are ripe, harvest them, cut the fruits, and count the incidence of seedless fruits.

[0027] In the specific implementation process of the above embryo-promoting method in this embodiment, a group of mangoes that did not hang and apply the embryo-promoting solution I during the inflorescence development period, the embryo-promoting solution II during the full-bloom period, the embryo-promoting solution III during the fruit swelling period, and the embryo-promoting solution IV after the swelling period was used as the blank control group; A group of mangoes that only hung and applied the high-concentration embryo-promoting solution I during the inflorescence development period was used as experimental group one; A group of mangoes that only hung and applied the high-concentration embryo-promoting solution II during the full-bloom period was used as experimental group two; A group of mangoes that only hung and applied the high-concentration embryo-promoting solution III during the fruit swelling period was used as experimental group three; A group of mangoes that only hung and applied the high-concentration embryo-promoting solution IV after the swelling period was used as experimental group four; A group of mangoes that hung and applied the high-concentration embryo-promoting solution I during the inflorescence development period and the high-concentration embryo-promoting solution II during the full-bloom period was used as experimental group five (that is, did not hang and apply the embryo-promoting solution III during the fruit swelling period and the embryo-promoting solution IV after the swelling period); A group of mangoes that hung and applied the high-concentration embryo-promoting solution I during the inflorescence development period and the high-concentration embryo-promoting solution III during the fruit swelling period was used as experimental group six (that is, did not hang and apply the embryo-promoting solution II during the full-bloom period and the embryo-promoting solution IV after the swelling period); A group of mangoes that hung and applied the high-concentration embryo-promoting solution I during the inflorescence development period and the embryo-promoting solution IV after the swelling period was used as experimental group seven (that is, did not hang and apply the embryo-promoting solution II during the full-bloom period and the embryo-promoting solution III during the fruit swelling period); A group of mangoes that hung and applied the high-concentration embryo-promoting solution I during the inflorescence development period, the high-concentration embryo-promoting solution II during the full-bloom period, and the high-concentration embryo-promoting solution III during the fruit swelling period was used as experimental group eight (that is, did not hang and apply the embryo-promoting solution IV after the swelling period); The mango group that was drip-fed with the high-concentration embryo-promoting solution I during the inflorescence development period, the high-concentration embryo-promoting solution II during the full-bloom period, and the high-concentration embryo-promoting solution IV after the swelling period was the ninth experimental group (i.e., the fruit swelling period embryo-promoting solution III was not drip-fed). The mango group that was drip-fed with the high-concentration embryo-promoting solution I during the inflorescence development period, the high-concentration embryo-promoting solution III during the fruit swelling period, and the high-concentration embryo-promoting solution IV after the swelling period was the tenth experimental group (i.e., the full-bloom period embryo-promoting solution II was not drip-fed). The mango group that was drip-fed with the high-concentration embryo-promoting solution II during the full-bloom period, the high-concentration embryo-promoting solution III during the fruit swelling period, and the high-concentration embryo-promoting solution IV after the swelling period was the eleventh experimental group (i.e., the inflorescence development period embryo-promoting solution I was not drip-fed). The mango group that was drip-fed with the high-concentration embryo-promoting solution I during the inflorescence development period, the high-concentration embryo-promoting solution II during the full-bloom period, the high-concentration embryo-promoting solution III during the fruit swelling period, and the high-concentration embryo-promoting solution IV after the swelling period was the twelfth experimental group.

[0028] It was found that the incidence of seedless fruits in the blank control group of mangoes was 90%, the incidence of seedless fruits in the mangoes of the first experimental group was 85%, the incidence of seedless fruits in the mangoes of the second experimental group was 87%, the incidence of seedless fruits in the mangoes of the third experimental group was 86%, the incidence of seedless fruits in the mangoes of the fourth experimental group was 88%, the incidence of seedless fruits in the mangoes of the fifth experimental group was 80%, the incidence of seedless fruits in the mangoes of the sixth experimental group was 83%, the incidence of seedless fruits in the mangoes of the seventh experimental group was 78%, the incidence of seedless fruits in the mangoes of the eighth experimental group was 73%, the incidence of seedless fruits in the mangoes of the ninth experimental group was 75%, the incidence of seedless fruits in the mangoes of the tenth experimental group was 77%, the incidence of seedless fruits in the mangoes of the eleventh experimental group was 74%, and the incidence of seedless fruits in the mangoes of the twelfth experimental group was 73%. Thus, it can be seen that using the mango embryo-promoting agent of the present invention can reduce the incidence of seedless fruits in mangoes, especially when the mango embryo-promoting agents of the present invention are used in combination, the incidence of seedless fruits in mangoes can be significantly reduced.

[0029] Example 2 A method for promoting embryo development in mangoes This example is a method for promoting embryo development in mangoes, and the specific process includes the following steps: I. Preparation of the mango embryo-promoting agent In this example, the mango embryo-promoting agent includes the embryo-promoting solution I during the inflorescence development period, the embryo-promoting solution II during the full-bloom period, the embryo-promoting solution III during the fruit swelling period, and the embryo-promoting solution IV after the swelling period. Its formula and preparation process are basically the same as those in Example 1, except that: During the preparation of the embryo-promoting solution II at three different concentrations (low, medium, and high) during the full-bloom stage, gibberellin A4 (GA4) was used instead of gibberellin A3 (GA3). Among them, the concentrations of GA4 in the embryo-promoting solution II at the full-bloom stage with low, medium, and high concentrations were 20, 30, and 40 mg / L in sequence. The types, dosages, and preparation processes of other raw materials in the embryo-promoting solution II at the full-bloom stage with low, medium, and high concentrations were exactly the same as those in Example 1.

[0030] II. Embryo-promoting method During the cultivation of mango "Guifei", the above-mentioned mango embryo-promoting agent was applied by hanging according to the embryo-promoting method in Example 1. The mango group without applying the embryo-promoting solution I during the inflorescence development stage, the embryo-promoting solution II during the full-bloom stage, the embryo-promoting solution III during the fruit expansion stage, and the embryo-promoting solution IV after the expansion stage was used as the blank control group; The mango group only applying the low-concentration embryo-promoting solution I during the inflorescence development stage was used as Experimental Group 1; The mango group only applying the low-concentration embryo-promoting solution II during the full-bloom stage was used as Experimental Group 2; The mango group only applying the low-concentration embryo-promoting solution III during the fruit expansion stage was used as Experimental Group 3; The mango group only applying the low-concentration embryo-promoting solution IV after the expansion stage was used as Experimental Group 4; The mango group applying the low-concentration embryo-promoting solution I during the inflorescence development stage and the low-concentration embryo-promoting solution II during the full-bloom stage was used as Experimental Group 5 (i.e., without applying the embryo-promoting solution III during the fruit expansion stage and the embryo-promoting solution IV after the expansion stage); The mango group applying the low-concentration embryo-promoting solution I during the inflorescence development stage and the low-concentration embryo-promoting solution III during the fruit expansion stage was used as Experimental Group 6 (i.e., without applying the embryo-promoting solution II during the full-bloom stage and the embryo-promoting solution IV after the expansion stage); The mango group applying the low-concentration embryo-promoting solution I during the inflorescence development stage and the embryo-promoting solution IV after the expansion stage was used as Experimental Group 7 (i.e., without applying the embryo-promoting solution II during the full-bloom stage and the embryo-promoting solution III during the fruit expansion stage); The mango group applying the low-concentration embryo-promoting solution I during the inflorescence development stage, the low-concentration embryo-promoting solution II during the full-bloom stage, and the low-concentration embryo-promoting solution III during the fruit expansion stage was used as Experimental Group 8 (i.e., without applying the embryo-promoting solution IV after the expansion stage); The mango group applying the low-concentration embryo-promoting solution I during the inflorescence development stage, the low-concentration embryo-promoting solution II during the full-bloom stage, and the low-concentration embryo-promoting solution IV after the expansion stage was used as Experimental Group 9 (i.e., without applying the embryo-promoting solution III during the fruit expansion stage); The mango group applying the low-concentration embryo-promoting solution I during the inflorescence development stage, the low-concentration embryo-promoting solution III during the fruit expansion stage, and the low-concentration embryo-promoting solution IV after the expansion stage was used as Experimental Group 10 (i.e., without applying the embryo-promoting solution II during the full-bloom stage); The mango group that was drip-fed with the low-concentration embryo-promoting solution II during the full-bloom period, the low-concentration embryo-promoting solution III during the fruit expansion period, and the low-concentration embryo-promoting solution IV after the expansion period was designated as experimental group 11 (i.e., the embryo-promoting solution I during the inflorescence development period was not drip-fed). The mango group that was drip-fed with the low-concentration embryo-promoting solution I during the inflorescence development period, the low-concentration embryo-promoting solution II during the full-bloom period, the low-concentration embryo-promoting solution III during the fruit expansion period, and the low-concentration embryo-promoting solution IV after the expansion period was designated as experimental group 12. Among them, the comparison diagrams of embryos-containing fruits and embryos-free fruits are as Figure 3 shown, and the statistical charts of embryos-containing fruits and embryos-free fruits are as Figure 4 shown; the inspection of mango embryo development is as Figure 5 and Figure 6 shown.

[0031] It was found that the incidence of embryos-free fruits in the blank control group was 92%, the incidence of embryos-free fruits in experimental group 1 was 89%, the incidence of embryos-free fruits in experimental group 2 was 90%, the incidence of embryos-free fruits in experimental group 3 was 88%, the incidence of embryos-free fruits in experimental group 4 was 87%, the incidence of embryos-free fruits in experimental group 5 was 85%, the incidence of embryos-free fruits in experimental group 6 was 83%, the incidence of embryos-free fruits in experimental group 7 was 86%, the incidence of embryos-free fruits in experimental group 8 was 84%, the incidence of embryos-free fruits in experimental group 9 was 83%, the incidence of embryos-free fruits in experimental group 10 was 82%, the incidence of embryos-free fruits in experimental group 11 was 84%, and the incidence of embryos-free fruits in experimental group 12 was 81%. Thus, it can be seen that using the mango embryo-promoting agent of the present invention can reduce the incidence of embryos-free fruits in mangoes. Especially when the mango embryo-promoting agents of the present invention are used in combination, the incidence of embryos-free fruits in mangoes can be significantly reduced.

[0032] Example 3 A method for promoting embryo development in mangoes This example is a method for promoting embryo development in mangoes, and the specific process includes the following steps: I. Preparation of the mango embryo-promoting agent In this example, the mango embryo-promoting agent includes the embryo-promoting solution I during the inflorescence development period, the embryo-promoting solution II during the full-bloom period, the embryo-promoting solution III during the fruit expansion period, and the embryo-promoting solution IV after the expansion period. Its formula and preparation process are basically the same as those in Example 1, except that: During the preparation of the low-, medium-, and high-concentration embryo-promoting solution II during the full-bloom period, gibberellin A7 (GA7) was used instead of gibberellin A7 (GA7). Among them, the concentrations of GA7 in the low-, medium-, and high-concentration embryo-promoting solution II during the full-bloom period were 20, 30, and 40 mg / L in sequence. The types, dosages, and preparation processes of other raw materials in the low-, medium-, and high-concentration embryo-promoting solution II during the full-bloom period were exactly the same as those in Example 1.

[0033] II. Method for promoting embryo development AsFigure 7 As shown in Figure 7 , during the cultivation process of mango "Jinhuang", the above-mentioned mango embryo-promoting agent was applied by hanging according to the embryo-promoting method in Example 1. A mango group that did not apply the embryo-promoting solution I during the inflorescence development period, the embryo-promoting solution II during the full-bloom period, the embryo-promoting solution III during the fruit enlargement period, and the embryo-promoting solution IV after the enlargement period was used as the blank control group; A mango group that only applied the medium-concentration embryo-promoting solution I during the inflorescence development period was used as Experimental Group 1; A mango group that only applied the medium-concentration embryo-promoting solution II during the full-bloom period was used as Experimental Group 2; A mango group that only applied the medium-concentration embryo-promoting solution III during the fruit enlargement period was used as Experimental Group 3; A mango group that only applied the medium-concentration embryo-promoting solution IV after the enlargement period was used as Experimental Group 4; A mango group that applied the medium-concentration embryo-promoting solution I during the inflorescence development period and the medium-concentration embryo-promoting solution II during the full-bloom period was used as Experimental Group 5 (i.e., did not apply the embryo-promoting solution III during the fruit enlargement period and the embryo-promoting solution IV after the enlargement period); A mango group that applied the medium-concentration embryo-promoting solution I during the inflorescence development period and the medium-concentration embryo-promoting solution III during the fruit enlargement period was used as Experimental Group 6 (i.e., did not apply the embryo-promoting solution II during the full-bloom period and the embryo-promoting solution IV after the enlargement period); A mango group that applied the medium-concentration embryo-promoting solution I during the inflorescence development period and the embryo-promoting solution IV after the enlargement period was used as Experimental Group 7 (i.e., did not apply the embryo-promoting solution II during the full-bloom period and the embryo-promoting solution III during the fruit enlargement period); A mango group that applied the medium-concentration embryo-promoting solution I during the inflorescence development period, the medium-concentration embryo-promoting solution II during the full-bloom period, and the medium-concentration embryo-promoting solution III during the fruit enlargement period was used as Experimental Group 8 (i.e., did not apply the embryo-promoting solution IV after the enlargement period); A mango group that applied the medium-concentration embryo-promoting solution I during the inflorescence development period, the medium-concentration embryo-promoting solution II during the full-bloom period, and the medium-concentration embryo-promoting solution IV after the enlargement period was used as Experimental Group 9 (i.e., did not apply the embryo-promoting solution III during the fruit enlargement period); A mango group that applied the medium-concentration embryo-promoting solution I during the inflorescence development period, the medium-concentration embryo-promoting solution III during the fruit enlargement period, and the medium-concentration embryo-promoting solution IV after the enlargement period was used as Experimental Group 10 (i.e., did not apply the embryo-promoting solution II during the full-bloom period); A mango group that applied the medium-concentration embryo-promoting solution II during the full-bloom period, the medium-concentration embryo-promoting solution III during the fruit enlargement period, and the medium-concentration embryo-promoting solution IV after the enlargement period was used as Experimental Group 11 (i.e., did not apply the embryo-promoting solution I during the inflorescence development period); A mango group that applied the medium-concentration embryo-promoting solution I during the inflorescence development period, the medium-concentration embryo-promoting solution II during the full-bloom period, the medium-concentration embryo-promoting solution III during the fruit enlargement period, and the medium-concentration embryo-promoting solution IV after the enlargement period was used as Experimental Group 12.

[0034] It was found that the incidence rate of seedless mango fruits in the blank control group was 93%, that in experimental group 1 was 90%, that in experimental group 2 was 89%, that in experimental group 3 was 91%, that in experimental group 4 was 90%, that in experimental group 5 was 88%, that in experimental group 6 was 89%, that in experimental group 7 was 87%, that in experimental group 8 was 86%, that in experimental group 9 was 85%, that in experimental group 10 was 83%, that in experimental group 11 was 82%, and that in experimental group 12 was 80%. Thus, it can be seen that using the mango embryo-promoting agent of the present invention can reduce the incidence rate of seedless mango fruits. Especially when the mango embryo-promoting agent of the present invention is used in combination, the incidence rate of seedless mango fruits can be significantly reduced.

[0035] Example 4 A method for promoting embryo development of mango This example is a method for promoting embryo development of mango, and the specific process includes the following steps: I. Preparation of mango embryo-promoting agent In this example, the mango embryo-promoting agent includes embryo-promoting solution I during the inflorescence development period, embryo-promoting solution II during the full-bloom period, embryo-promoting solution III during the fruit swelling period, and embryo-promoting solution IV after the swelling period. Its formula and preparation process are exactly the same as those in Example 1.

[0036] II. Method for promoting embryo development During the cultivation of mango 'Kate', the above-mentioned mango embryo-promoting agent was applied by hanging according to the embryo-promoting method in Example 1. The mango group without applying embryo-promoting solution I during the inflorescence development period, embryo-promoting solution II during the full-bloom period, embryo-promoting solution III during the fruit swelling period, and embryo-promoting solution IV after the swelling period was used as the blank control group; The mango group only applying medium-concentration embryo-promoting solution I during the inflorescence development period was used as experimental group 1; The mango group only applying medium-concentration embryo-promoting solution II during the full-bloom period was used as experimental group 2; The mango group only applying medium-concentration embryo-promoting solution III during the fruit swelling period was used as experimental group 3; The mango group only applying medium-concentration embryo-promoting solution IV after the swelling period was used as experimental group 4; The mango group applying medium-concentration embryo-promoting solution I during the inflorescence development period and medium-concentration embryo-promoting solution II during the full-bloom period was used as experimental group 5 (i.e., without applying embryo-promoting solution III during the fruit swelling period and embryo-promoting solution IV after the swelling period); The mango group applying medium-concentration embryo-promoting solution I during the inflorescence development period and medium-concentration embryo-promoting solution III during the fruit swelling period was used as experimental group 6 (i.e., without applying embryo-promoting solution II during the full-bloom period and embryo-promoting solution IV after the swelling period); The mango group with medium - concentration embryo - promoting solution I during the inflorescence development stage and embryo - promoting solution IV after the swelling stage is used as the seventh experimental group (i.e., without applying embryo - promoting solution II during the full - bloom stage and embryo - promoting solution III during the fruit - swelling stage); The mango group with medium - concentration embryo - promoting solution I during the inflorescence development stage, medium - concentration embryo - promoting solution II during the full - bloom stage, and medium - concentration embryo - promoting solution III during the fruit - swelling stage is used as the eighth experimental group (i.e., without applying embryo - promoting solution IV after the swelling stage); The mango group with medium - concentration embryo - promoting solution I during the inflorescence development stage, medium - concentration embryo - promoting solution II during the full - bloom stage, and medium - concentration embryo - promoting solution IV after the swelling stage is used as the ninth experimental group (i.e., without applying embryo - promoting solution III during the fruit - swelling stage); The mango group with medium - concentration embryo - promoting solution I during the inflorescence development stage, medium - concentration embryo - promoting solution III during the fruit - swelling stage, and medium - concentration embryo - promoting solution IV after the swelling stage is used as the tenth experimental group (i.e., without applying embryo - promoting solution II during the full - bloom stage); The mango group with medium - concentration embryo - promoting solution II during the full - bloom stage, medium - concentration embryo - promoting solution III during the fruit - swelling stage, and medium - concentration embryo - promoting solution IV after the swelling stage is used as the eleventh experimental group (i.e., without applying embryo - promoting solution I during the inflorescence development stage); The mango group with medium - concentration embryo - promoting solution I during the inflorescence development stage, medium - concentration embryo - promoting solution II during the full - bloom stage, medium - concentration embryo - promoting solution III during the fruit - swelling stage, and medium - concentration embryo - promoting solution IV after the swelling stage is used as the twelfth experimental group. Among them, the comparison diagrams of embryos - containing fruits and embryos - absent fruits are as Figure 8 shown, and the statistical diagrams of embryos - containing fruits and embryos - absent fruits are as Figure 9 shown.

[0037] It is found that the incidence rate of embryos - absent fruits in the blank control group of mangoes is 94%, that in the first experimental group is 90%, that in the second experimental group is 91%, that in the third experimental group is 89%, that in the fourth experimental group is 90%, that in the fifth experimental group is 87%, that in the sixth experimental group is 85%, that in the seventh experimental group is 86%, that in the eighth experimental group is 85%, that in the ninth experimental group is 84%, that in the tenth experimental group is 82%, that in the eleventh experimental group is 84%, and that in the twelfth experimental group is 78%. Thus, it can be seen that using the mango embryo - promoting agent of the present invention can reduce the incidence rate of embryos - absent fruits in mangoes. Especially when using the mango embryo - promoting agent of the present invention in combination, the incidence rate of embryos - absent fruits in mangoes can be significantly reduced.

[0038] Examples 5 - 6 Mango Embryo - Promoting Agent Examples 5 - 6 are respectively a kind of mango embryo - promoting agent. Their preparation steps are basically the same as those in Example 1, except for the different raw material ratios. For details, see Table 1: Table 1 List of Process Parameters in Examples 5 - 6

[0039] For Examples 5 - 6, the content of other parts is the same as that of Example 1.

[0040] Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the scope of protection of the present invention.

Claims

1. A mango embryo-promoting agent, characterized in that, The mango embryo-promoting agent includes the embryo-promoting solution I during the inflorescence development stage; The embryo-promoting solution I during the inflorescence development stage uses MS culture medium as the solvent, and contains aminoethoxyvinylglycine at a concentration of 10 - 130 ppm, gibberellin A3 at a concentration of 20 - 300 ppm, imidacloprid at a concentration of 1 - 10 g / L, spinetoram at a concentration of 10 - 100 g / L, 6-benzylaminopurine at a concentration of 10 - 200 mg / L, and KH2PO4 at a concentration of 200 - 2000 mg / L.

2. The mango embryo-promoting agent according to claim 1, characterized in that, The mango embryo-promoting agent further includes the embryo-promoting solution II during the full-bloom stage; The formula of the embryo-promoting solution II during the full-bloom stage includes: MS culture medium, aminoethoxyvinylglycine, imidacloprid, spinetoram, gibberellin, and compound amino acid trace element chelate.

3. The mango embryo-promoting agent according to claim 2, wherein, The gibberellin used in the embryo-promoting solution II during the full-bloom stage is gibberellin A3, gibberellin A4, or gibberellin A7.

4. The mango embryo-promoting agent according to claim 2 or 3, characterized in that The embryo-promoting solution II during the full-bloom stage uses MS culture medium as the solvent, wherein the concentration of aminoethoxyvinylglycine is 10 - 130 ppm, the concentration of imidacloprid is 1 - 10 g / L, the concentration of spinetoram is 10 - 100 g / L, the concentration of gibberellin is 10 - 50 mg / L, and the concentration of compound amino acid trace element chelate is 500 - 2500 mg / L.

5. The mango embryo-promoting agent according to any one of claims 1-3, characterized in that, The mango embryo-promoting agent further includes the embryo-promoting solution III during the fruit expansion stage; The formula of the embryo-promoting solution III during the fruit expansion stage includes: MS culture medium, aminoethoxyvinylglycine, gibberellin A3, imidacloprid, spinetoram, indoleacetic acid, and urea.

6. The mango embryo-promoting agent according to claim 5, wherein The embryo-promoting solution III during the fruit expansion stage uses MS culture medium as the solvent, wherein the concentration of aminoethoxyvinylglycine is 10 - 130 ppm, the concentration of gibberellin A3 is 20 - 300 ppm, the concentration of imidacloprid is 1 - 10 g / L, the concentration of spinetoram is 10 - 100 g / L, the concentration of indoleacetic acid is 20 - 100 mg / L, and the concentration of urea is 1000 - 5000 mg / L.

7. The mango embryo-promoting agent according to claim 1, 2, 3 or 6, characterized in that The mango embryo-promoting agent further includes the embryo-promoting solution IV after the expansion stage; The formula of the embryo-promoting solution IV after the expansion stage includes: MS culture medium, aminoethoxyvinylglycine, gibberellin A3, imidacloprid, spinetoram, indoleacetic acid, and compound amino acid trace element chelate.

8. The mango embryo-promoting agent according to claim 7, characterized in that, The embryo-promoting solution IV after the expansion stage uses MS culture medium as the solvent, wherein the concentration of aminoethoxyvinylglycine is 10 - 130 ppm, the concentration of gibberellin A is 20 - 300 ppm, the concentration of imidacloprid is 1 - 10 g / L, the concentration of spinetoram is 10 - 100 g / L, the concentration of indoleacetic acid is 20 - 100 mg / L, and the concentration of compound amino acid trace element chelate is 500 - 3000 mg / L.

9. A method for promoting embryo of mango, characterized in that, The method for promoting embryo development of mango is to apply the mango embryo-promoting agent according to any one of claims 1 - 8 during the mango planting process to promote the embryo development of mango.

10. The method for promoting embryo of mango according to claim 9, characterized in that, The mango embryo-promoting agent includes embryo-promoting solution I during the inflorescence development period, embryo-promoting solution II during the full-bloom period, embryo-promoting solution III during the fruit swelling period, and embryo-promoting solution IV after the swelling period; During the mango planting process, the embryo-promoting solution I during the inflorescence development period is applied at the initial stage of inflorescence development; The embryo-promoting solution II during the full-bloom period is applied during the full-bloom period; The embryo-promoting solution III during the fruit swelling period is applied during the fruit swelling period; The embryo-promoting solution IV after the swelling period is applied 15 days after the swelling period; The embryo-promoting solution I during the inflorescence development period uses MS culture solution as a solvent. Among them, the concentration of aminoethoxyvinylglycine is 10-130 ppm, the concentration of gibberellin A3 is 20-300 ppm, the concentration of imidacloprid is 1-10 g / L, the concentration of spinetoram is 10-100 g / L, the concentration of 6-benzylaminopurine is 10-200 mg / L, and the concentration of KH2PO4 is 200-2000 mg / L; The embryo-promoting solution II during the full-bloom period uses MS culture solution as a solvent. Among them, the concentration of aminoethoxyvinylglycine is 10-130 ppm, the concentration of imidacloprid is 1-10 g / L, the concentration of spinetoram is 10-100 g / L, the concentration of gibberellin is 10-50 mg / L, and the concentration of compound amino acid trace element chelate is 500-2500 mg / L; The gibberellin used in the embryo-promoting solution II during the full-bloom period is gibberellin A3, gibberellin A4 or gibberellin A7; The embryo-promoting solution III during the fruit swelling period uses MS culture solution as a solvent. Among them, the concentration of aminoethoxyvinylglycine is 10-130 ppm, the concentration of gibberellin A3 is 20-300 ppm, the concentration of imidacloprid is 1-10 g / L, the concentration of spinetoram is 10-100 g / L, the concentration of indoleacetic acid is 20-100 mg / L, and the concentration of urea is 1000-5000 mg / L; The embryo-promoting solution IV after the swelling period uses MS culture solution as a solvent. Among them, the concentration of aminoethoxyvinylglycine is 10-130 ppm, the concentration of gibberellin A is 20-300 ppm, the concentration of imidacloprid is 1-10 g / L, the concentration of spinetoram is 10-100 g / L, the concentration of indoleacetic acid is 20-100 mg / L, and the concentration of compound amino acid trace element chelate is 500-3000 mg / L.

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