Black bud mulberry plant beverage for enhancing immunity and tonifying qi and blood

Abstract

The invention provides a black bud mulberry plant beverage for enhancing immunity and tonifying qi and blood, and relates to the technical field of food beverages, and the black bud mulberry plant beverage is prepared from the following raw materials: peanut buds, black peanut buds, red beans, red dates, brown sugar, Chinese wolfberry fruits, mulberries, blueberries, black fungi, tremella, black beans, chrysanthemums and water. The peanut sprouts and the black peanut sprouts are rich in protein and various essential amino acids for a human body, provide basic materials for constructing and repairing tissues for the body, and are beneficial to maintaining normal metabolism and functions of immune system cells, so that the immunity is enhanced; lycium barbarum, mulberries and tremella fuciformis are rich in polysaccharide components, and polysaccharide can regulate an immune system, enhance the phagocytic ability of macrophages and promote proliferation and differentiation of lymphocytes, so that the immune function of a body is improved, the activity of immune cells is enhanced, and the resistance of a human body to pathogens is improved; the health-care tea is also beneficial to promoting the generation of qi and blood, and has the effects of tonifying qi and benefiting blood for drinkers.

Description

Technical Field

[0001] The present invention relates to the technical field of food and beverages, and particularly to a black sprout mulberry plant drink for enhancing immunity, tonifying qi and nourishing blood. Background Art

[0002] In today's fast-paced living environment, people are facing increasing work pressure, irregular work and rest schedules, and unbalanced eating habits. These factors have many negative impacts on physical health, resulting in more common problems such as decreased immunity and insufficient qi and blood.

[0003] Immunity, as the body's own defense system, can not only recognize and resist the invasion of foreign viruses, bacteria and other pathogens, but also be responsible for dealing with senescent, damaged, dead and denatured cells in the body to maintain the stability of the body's internal environment. However, the modern lifestyle has overburdened the immune systems of many people, resulting in symptoms such as frequent illness, easy fatigue, and slow recovery. Insufficient qi and blood can cause a series of discomfort symptoms, seriously affecting the quality of life.

[0004] At present, there are many types of products on the market for enhancing immunity and tonifying qi and nourishing blood, but most of them are chemically synthesized drugs. Long-term use may bring certain side effects, such as immune function disorders, drug resistance and other problems. Their ingredients are complex, the quality is uneven, and the prices are expensive. Traditional Chinese medicine has a certain curative effect on tonifying qi and nourishing blood, but it often requires a cumbersome decoction process and is inconvenient to take. Summary of the Invention

[0005] The purpose of the present invention is to provide a black sprout mulberry plant drink for enhancing immunity, tonifying qi and nourishing blood, and solve the technical problems existing in the prior art.

[0006] In order to achieve the above-mentioned invention purpose, the technical solution adopted by the present invention is as follows:

[0007] A black sprout mulberry plant drink for enhancing immunity, tonifying qi and nourishing blood is made from the following raw materials: peanut sprouts, black peanut sprouts, red beans, red dates, brown sugar, wolfberries, mulberries, blueberries, black fungus, tremella, black beans, chrysanthemums and water.

[0008] Further, the weights of the raw materials are: 100 - 250 g of peanut sprouts, 10 - 35 g of black peanut sprouts, 10 - 25 g of red beans, 5 - 20 g of red dates, 10 - 25 g of brown sugar, 5 - 15 g of wolfberries, 6 - 16 g of mulberries, 11 - 21 g of blueberries, 4 - 13 g of black fungus, 5 - 15 g of tremella, 1 - 6 g of black beans, 3 - 10 g of chrysanthemums and 5 - 20 g of water.

[0009] Further, the weights of the raw materials are as follows: germinated seeds 150 - 200 g, black peanut sprouts 15 - 20 g, red beans 12 - 15 g, red dates 7 - 10 g, brown sugar 12 - 15 g, wolfberries 8 - 10 g, mulberries 8 - 10 g, blueberries 13 - 15 g, black fungus 10 - 12 g, tremella 7 - 10 g, black beans 2 - 3 g, chrysanthemums 4 - 5 g, and water 8 - 10 g. Compared with the prior art, the present invention has the following beneficial effects:

[0010] (1) In the present invention, peanut sprouts and black peanut sprouts are rich in protein and various essential amino acids for the human body, providing the basic materials for building and repairing tissues in the body, helping to maintain the normal metabolism and functions of immune system cells, and thus enhancing immunity; mulberries and blueberries play an antioxidant role, and the anthocyanins therein can scavenge free radicals in the body, reduce the damage of oxidative stress to cells, help maintain the normal functions of cells, delay aging, and indirectly enhance immunity; wolfberries, mulberries, and tremella are rich in polysaccharide components. Polysaccharides can regulate the immune system, enhance the phagocytic ability of macrophages, and promote the proliferation and differentiation of lymphocytes, thereby improving the body's immune function, enhancing the activity of immune cells, and enhancing the body's resistance to pathogens; in the present invention, red beans, red dates, and black fungus are all ingredients rich in iron. Iron is a key raw material for synthesizing hemoglobin, helping to improve the oxygen-carrying capacity of the blood, alleviating symptoms such as pale complexion, dizziness, and fatigue caused by qi and blood deficiency, promoting the generation of qi and blood, and playing a role in supplementing qi and nourishing blood for drinkers;

[0011] (2) The formula of the present invention uses all natural raw materials and has a reasonable compatibility. It can not only enhance immunity and supplement qi and nourish blood, but also has no side effects, will not cause harm to the human body, and is convenient to drink. It is suitable for a wide range of people and has a broad market prospect. Specific Embodiments

[0012] In order to make the objectives, technical solutions, and technical effects of the present invention clearer and more understandable, the following describes specific embodiments of the present invention. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0013] In this embodiment, a black sprout mulberry plant drink for enhancing immunity and supplementing qi and nourishing blood is provided, which is made from the following raw materials: peanut sprouts, black peanut sprouts, red beans, red dates, brown sugar, wolfberries, mulberries, blueberries, black fungus, tremella, black beans, chrysanthemums, and water. The specific effects are as follows:

[0014] Peanut sprouts: Improve the activity of immune cells, promote blood generation, and enhance physical strength;

[0015] Black peanut sprouts: Improve blood circulation and enhance the body's resistance;

[0016] Red beans: Contribute to the synthesis of hemoglobin, promote blood generation, and enhance the function of the immune system;

[0017] Red dates: Rich in carbohydrates and vitamins, they help strengthen qi and blood, improve sleep quality, and thus indirectly enhance immunity;

[0018] Brown sugar: Provides energy for the body, enhances physical strength, promotes digestion, helps the body absorb more nutrients, and boosts immunity;

[0019] Wolfberries: Rich in antioxidants, they can enhance the immune system, improve the overall health of the body, and promote the generation of qi and blood;

[0020] Mulberries: Rich in ingredients such as iron and vitamin C, they help nourish the blood, strengthen qi and blood, and have good antioxidant capacity, enhancing immune function;

[0021] Blueberries: Effectively resist free radicals, enhance immunity, improve microcirculation, and help boost qi and blood;

[0022] Black fungus: Helps remove toxins from the body, keeps the immune system healthy, reduces blood lipids, and maintains cardiovascular health;

[0023] Tremella: Has a good nourishing effect on the lungs and kidneys, indirectly promotes immunity, enhances the body's metabolic capacity, and supplements nutrients;

[0024] Black beans: Rich in protein and minerals, they can promote blood generation, and the antioxidants in them help improve immunity;

[0025] Chrysanthemums: Have the effect of clearing heat and detoxifying, help improve the response ability of the immune system, and contribute to improving blood circulation and providing more nutrients.

[0026] Example 1:

[0027] The weights of the raw materials are as follows: 100 g of peanut sprouts, 10 g of black peanut sprouts, 5 g of red dates, 10 g of brown sugar, 5 g of wolfberries, 6 g of mulberries, 11 g of blueberries, 4 g of black fungus, 5 g of tremella, 1 g of black beans, 3 g of chrysanthemums, and 400 g of water;

[0028] Its preparation process is as follows:

[0029] S1: Weigh peanut sprouts, black peanut sprouts, red dates, brown sugar, wolfberries, mulberries, blueberries, black fungus, tremella, black beans, and chrysanthemums according to the corresponding weights; soak the black beans in clean water for 2 - 5 h until they are fully swollen; soak the tremella in clean water for 30 - 50 min until it is completely swollen and soft, and tear it into small pieces; thoroughly rinse the peanut sprouts, black peanut sprouts, wolfberries, mulberries, blueberries, black fungus, and chrysanthemums with running water to remove impurities and dirt; remove the pits from the red dates and cut them into small pieces; soak the black fungus for 1 - 2 h until it is soaked and then cut it into small pieces;

[0030] S2: Put the soaked black beans in step S1 into a pot, add water in the corresponding proportion and bring to a boil, then turn to low heat and continue to cook for 30 - 55 minutes until the beans become soft; add peanut sprouts, black peanut sprouts, tremella, auricularia auricula, red dates, wolfberries, mulberries and blueberries to the pot, and continue to cook for 20 - 35 minutes to boil out a thick gelatinous soup; then add brown sugar and stir the mixture evenly.

[0031] S3: Filter the mixture in step S2 through a filtering device, add chrysanthemums to the filtered liquid and let it stand at room temperature for 15 - 25 minutes; then use a centrifuge to take the supernatant at a rotational speed of 3500 - 4500 r / min.

[0032] S4: Pasteurize and fill the supernatant in step S3, and store the filled finished product refrigerated for later use.

[0033] Example Two:

[0034] The weights of the raw materials are as follows: 250 g of peanut sprouts, 35 g of black peanut sprouts, 25 g of red beans, 15 g of wolfberries, 16 g of mulberries, 21 g of blueberries, 13 g of auricularia auricula, 15 g of tremella, 6 g of black beans, 10 g of chrysanthemums and 800 g of water.

[0035] Its preparation process is as follows:

[0036] S1: Weigh peanut sprouts, black peanut sprouts, red beans, wolfberries, mulberries, blueberries, auricularia auricula, tremella, black beans and chrysanthemums according to the corresponding weights; soak the red beans and black beans in clean water for 2 - 5 hours until they are fully swollen; soak the tremella in clean water for 30 - 50 minutes until it is completely swollen and soft, and tear it into small pieces; rinse the peanut sprouts, black peanut sprouts, wolfberries, mulberries, blueberries, auricularia auricula and chrysanthemums thoroughly with running water to remove impurities and dirt; soak the auricularia auricula for 1 - 2 hours until it is soaked and cut into small pieces.

[0037] S2: Put the soaked red beans and black beans in step S1 into a pot, add water in the corresponding proportion and bring to a boil, then turn to low heat and continue to cook for 30 - 55 minutes until the beans become soft; add peanut sprouts, black peanut sprouts, tremella, auricularia auricula, wolfberries, mulberries and blueberries to the pot, and continue to cook for 20 - 35 minutes to boil out a thick gelatinous soup, and stir the mixture evenly.

[0038] S3: Filter the mixture in step S2 through a filtering device, add chrysanthemums to the filtered liquid and let it stand at room temperature for 15 - 25 minutes; then use a centrifuge to take the supernatant at a rotational speed of 3500 - 4500 r / min.

[0039] S4: Pasteurize and fill the supernatant in step S3, and store the filled finished product refrigerated for later use.

[0040] Example Three:

[0041] The weights of the raw materials are as follows: 150 g of peanut sprouts, 15 g of black peanut sprouts, 12 g of red beans, 7 g of red dates, 12 g of brown sugar, 8 g of wolfberries, 8 g of mulberries, 13 g of blueberries, 10 g of black fungus, 7 g of tremella, 2 g of black beans, 4 g of chrysanthemums, and 500 g of water;

[0042] Its preparation process is as follows:

[0043] S1: Weigh the peanut sprouts, black peanut sprouts, red beans, red dates, brown sugar, wolfberries, mulberries, blueberries, black fungus, tremella, black beans, and chrysanthemums according to the corresponding weights; soak the red beans and black beans in clear water for 2 h until they are fully swollen; soak the tremella in clear water for 30 min until it is fully swollen and soft, and tear it into small pieces; thoroughly rinse the peanut sprouts, black peanut sprouts, wolfberries, mulberries, blueberries, black fungus, and chrysanthemums with running water to remove impurities and dirt; remove the pits from the red dates and cut them into small pieces; soak the black fungus for 1 h until it is soaked and then cut it into small pieces;

[0044] S2: Put the soaked red beans and black beans in step S1 into a pot, add the corresponding proportion of water and bring to a boil, then turn to low heat and continue to cook for 30 min until the beans are soft; add the peanut sprouts, black peanut sprouts, tremella, black fungus, red dates, wolfberries, mulberries, and blueberries to the pot and continue to cook for 20 min to boil out a thick and gelatinous soup; then add brown sugar and stir the mixture evenly;

[0045] S3: Filter the mixture in step S2 through a filtering device, add chrysanthemums to the filtered liquid and let it stand at room temperature for 15 min; then use a centrifuge to take the supernatant at a rotation speed of 3500 r / min;

[0046] S4: Pasteurize and fill the supernatant in step S3, and refrigerate the filled finished product for later use.

[0047] Example 4:

[0048] The weights of the raw materials are as follows: 200 g of peanut sprouts, 20 g of black peanut sprouts, 15 g of red beans, 10 g of red dates, 15 g of brown sugar, 10 g of wolfberries, 10 g of mulberries, 15 g of blueberries, 12 g of black fungus, 10 g of tremella, 3 g of black beans, 5 g of chrysanthemums, and 600 g of water;

[0049] Its preparation process is as follows:

[0050] S1: Weigh the peanut sprouts, black peanut sprouts, red beans, red dates, brown sugar, wolfberries, mulberries, blueberries, black fungus, tremella, black beans, and chrysanthemums according to the corresponding weights; soak the red beans and black beans in clear water for 5 h until they are fully swollen; soak the tremella in clear water for 50 min until it is fully swollen and soft, and tear it into small pieces; thoroughly rinse the peanut sprouts, black peanut sprouts, wolfberries, mulberries, blueberries, black fungus, and chrysanthemums with running water to remove impurities and dirt; remove the pits from the red dates and cut them into small pieces; soak the black fungus for 2 h until it is soaked and then cut it into small pieces;

[0051] S2: Put the soaked red beans and black beans in step S1 into a pot, add water in the corresponding proportion and bring to a boil, then turn to low heat and continue to cook for 30 - 55 minutes until the beans become soft; add peanut sprouts, black peanut sprouts, tremella, black fungus, red dates, wolfberries, mulberries and blueberries to the pot and continue to cook for 35 minutes to boil out a thick gelatinous soup; then add brown sugar and stir the mixture evenly.

[0052] S3: Filter the mixture in step S2 through a filtering device, add chrysanthemum to the filtered liquid and let it stand at room temperature for 25 minutes; then use a centrifuge to take the supernatant at a rotational speed of 4500 r / min.

[0053] S4: Pasteurize and fill the supernatant in step S3, and refrigerate the filled finished product for standby.

[0054] In a further preferred embodiment, the present invention uses zebrafish as a model to conduct efficacy comparison and experiments on the above - mentioned scheme.

[0055] Experimental Materials and Methods

[0056] 1: Experimental animals: Randomly select 90 wild - type AB - strain zebrafish at 2 dpf and place them in a 5 - well plate, with 20 fish in each well.

[0057] 2: Grouping setting: Randomly divide the zebrafish into a normal control group, a model control group and an experimental group, with an equal number in each group.

[0058] 3: Model construction: Establish a zebrafish anemia model induced by phenylhydrazine.

[0059] 4: Treatment intervention: The experimental group is respectively given black - bud mulberry drink (the black - bud mulberry drink is injected through the fish - raising water), and at the same time, a normal control group and a model control group are set up, with a volume of 3 mL in each well; after the black - bud mulberry drink and phenylhydrazine are used to treat zebrafish for 18 h, stain with o - dianisidine. After staining, randomly select 10 zebrafish from each experimental group and take pictures and collect data under a dissection microscope, and analyze and statistically calculate the staining intensity S of cardiac red blood cells, and evaluate the improvement effect of black - bud mulberry drink on anemia based on the statistical significance of the staining intensity of cardiac red blood cells.

[0060] 5: Observation index: Observe the average staining intensity of cardiac red blood cells of zebrafish under a fluorescence microscope and take pictures.

[0061] Experimental Results:

[0062] As shown in the results in Table 1, the staining intensity of red blood cells in the hearts of zebrafish in the model control group was significantly decreased compared with that in the normal control group (P<0.001), indicating successful model establishment; the staining intensity of red blood cells in the hearts of zebrafish in the black bud mulberry drink group was 3988; the results showed that under the experimental conditions of this study, the black bud mulberry drink had an extremely obvious improvement effect on phenylhydrazine-induced anemia in zebrafish.

[0063] Table (1)

[0064] Group Heart red blood cell staining intensity (pixels) Normal control group 4580±128 Model control group 2380±60 Black bud mulberry drink group 4021±90***

[0065] Compared with the model control group, ***P<0.001; n = 10

[0066] Conclusion:

[0067] 1: After the comparative experiment with 20 zebrafish in each group, the number of red blood cells in the hearts of the zebrafish in the black bud mulberry drink group was similar to that in the normal control group without phenylhydrazine intake, and there was no decrease in the number of red blood cells in the hearts of the model control group;

[0068] 2: This experiment confirmed that the black bud mulberry drink has the efficacy of supplementing qi and nourishing blood.

[0069] In a further optimized embodiment, the present invention uses mice as a model to conduct the efficacy comparison and test of the above-mentioned scheme. The test materials and methods are as follows:

[0070] Experimental process

[0071] (1): Experimental animals: Clean-grade healthy mice, female, weighing 18 - 22 g. Breeding environment: Room temperature 23 + 2°C, relative humidity 40% - 70%. Randomly divided into 6 groups according to body weight, with 18 mice in each group, and the ConA-induced mouse spleen lymphocyte transformation experiment, DTH, spleen index and thymus index experiments were carried out respectively;

[0072] (2): Dose design: According to the recommended human intake of the black bud mulberry drink, the black bud mulberry drink was intragastrically administered at 0.5 g per day for 10 consecutive days.

[0073] Detection method

[0074] (1): Determination of organ / body weight ratio

[0075] The mice were continuously fed according to the dose design for 10 days, weighed once a week, and the initial, mid-term and final body weights were calculated. After being sacrificed by cervical dislocation, the thymus, spleen and liver were taken and weighed, and the thymus body weight ratio and spleen body weight ratio were calculated respectively.

[0076] (2): ConA-induced mouse spleen lymphocyte transformation test

[0077] Sterilely take the spleen, place it in sterile Hank's buffer solution to make a single-cell suspension. After filtration and washing, count the number of viable cells with a cell counter, with more than 95%, and adjust the cell concentration to 3×10 6 cfu / mL. Take two 1 mL spleen cell suspensions and add them to a cell culture plate respectively. Add 75 μL of ConA solution to one well, and the well with added culture medium is the control well. Incubate in an incubator at 37°C with 5% for 72 h. 4 h before the end of the culture, add 50 μL / well of MTT solution (5 mg / ml) and continue to culture for 4 h. Then, add 1 mL of acidic isopropanol to each well, pipette evenly to completely dissolve the purple crystals, and measure the optical density value at 570 nm with an enzyme-linked immunosorbent assay (ELISA) reader. Finally, subtract the optical density value of the well without ConA from the optical density value of the well with ConA to represent the proliferation ability of lymphocytes.

[0078] (3): After depilation of mice with DTH induced by dinitrofluorobenzene using depilatory cream, take 501 μL of 1% dinitrofluorobenzene solution and evenly apply it to the abdominal skin of the mice for the desensitization reaction. 5 days later, take 20 μL of 1% DNFB solution and evenly apply it to the right ear, with the left ear as the control. Sacrifice the mice 24 h later, and use a puncher to take ear pieces of the same size from the left and right ears for weighing. The difference in weight between the two ear pieces represents the degree of DTH. If the weight difference of the experimental group is significantly higher than that of the control group, it can be judged as positive.

[0079] Data statistics

[0080] Data are expressed as ±SD, and SPSS 20.0 software is used for statistical analysis of the results and to analyze the significance of differences between the experimental group and the control group.

[0081] Experimental results

[0082] Effect on the body weight and immune organ weight of mice

[0083] After 10 days of administration, there were no significant differences in the initial body weight and final body weight of the mice in the black bud mulberry drink group, as shown in Table 2; there were no significant differences in the thymus weight, spleen weight, thymus / body ratio, and spleen / body ratio of the mice in the black bud mulberry drink group, as shown in Table 3.

[0084] Table (2)

[0085] Grouping Initial weight (g) Final weight (g) Control group 20.45±1.59 2305±2.45 Black bud mulberry drink 21.84±1.87 25.45±2.38

[0086] Table (3)

[0087] Grouping Spleen (mg) <![CDATA[Spleen / body ratio × 10 -4 > Thymus (mg) <![CDATA[Thymus / body ratio × 10 -4 > Control group 73.45±6.59 2.38±0.20 57.87±4.98 2.02±0.14 Black bud mulberry drink 74.26±5.89 2.43±0.21 61.21±5.02 2.11±0.15

[0088] Effect on the cellular immune function of mice

[0089] Compared with the control group, there was a significant difference in the proliferation ability of spleen lymphocytes in the mice of the black bud mulberry drink group, OD570nm The value was significantly higher than that of the control group. The auricle swelling degree of the black bud mulberry drink group was significantly higher than that of the control group. The above results indicate that this group has a significant effect on the delayed hypersensitivity of mice. The results show that the black bud mulberry drink can enhance the immune function.

[0090]

[0091] Note: #P<0.05, there was a significant difference compared with the control group.

[0092] Conclusion

[0093] The black bud mulberry drink significantly improved the transformation of splenic lymphocytes and delayed hypersensitivity in mice. Therefore, the black bud mulberry drink group has the effect of enhancing immunity.

[0094] The above are only the preferred embodiments of the present invention patent, and are not intended to limit the present invention patent. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention patent shall be included within the protection scope of the present invention patent.

Claims

1. A black bud mulberry plant drink for enhancing immunity, supplementing qi and nourishing blood, characterized in that: It is made from the following raw materials: peanut sprouts, black peanut sprouts, red beans, red dates, brown sugar, wolfberries, mulberries, blueberries, black fungus, tremella, black beans, chrysanthemums and water.

2. The black bud mulberry plant drink for enhancing immunity and supplementing qi and nourishing blood according to claim 1, characterized in that: The weights of the raw materials are: 100 - 250 g of peanut sprouts, 10 - 35 g of black peanut sprouts, 10 - 25 g of red beans, 5 - 20 g of red dates, 10 - 25 g of brown sugar, 5 - 15 g of wolfberries, 6 - 16 g of mulberries, 11 - 21 g of blueberries, 4 - 13 g of black fungus, 5 - 15 g of tremella, 1 - 6 g of black beans, 3 - 10 g of chrysanthemums and 400 - 800 g of water.

3. The black bud mulberry plant drink for enhancing immunity, replenishing qi and nourishing blood according to claim 2, wherein: The weights of the raw materials are: 150 - 200 g of peanut sprouts, 15 - 20 g of black peanut sprouts, 12 - 15 g of red beans, 7 - 10 g of red dates, 12 - 15 g of brown sugar, 8 - 10 g of wolfberries, 8 - 10 g of mulberries, 13 - 15 g of blueberries, 10 - 12 g of black fungus, 7 - 10 g of tremella, 2 - 3 g of black beans, 4 - 5 g of chrysanthemums and 500 - 600 g of water.

Images