Composite peptide and application thereof in preparation of anti-aging products

Abstract

The invention provides a compound peptide composition with anti-aging and wrinkle-removing effects. The compound peptide composition with the anti-aging and anti-wrinkle effects is prepared from carnosine 1, carnosine, palmitoyl tetrapeptide-7, palmitoyl pentapeptide-4, hexapeptide-9, acetyl hexapeptide-8, palmitoyl tripeptide-1, palmitoyl tripeptide-5, snake venom-like tripeptide, palmitoyl hexapeptide-12, mannan, azone and ferulic acid. According to the polypeptide composition, polypeptide is used in cooperation and wrapped by lecithin, the synergistic interaction effect is achieved, the epidermis layer, the basal layer, the dermis layer and the muscle layer of skin can be repaired in a multi-target mode, then the composition can have the better wrinkle removing and anti-aging effect, and the functional requirements of consumers are met.

Description

Technical Field

[0001] The present invention belongs to the technical field of polypeptide applications, and particularly relates to a composite peptide and its application in the preparation of anti-aging products. Background Art

[0002] With the influence of internal factors such as diet and living habits as age increases, and being affected by the external contact environment, such as various stress factors like oxidation, dryness, and ultraviolet rays, the skin is prone to aging and is easily damaged. Since the skin is on the outermost side of the human body and clearly shows the beauty of the human body. Therefore, anti-aging cosmetics have become one of the products urgently needed by the majority of consumers.

[0003] A polypeptide is a protein molecule composed of multiple amino acids. It can promote the production and repair of collagen, improve skin elasticity, moisture retention, and gloss, and can also fade fine lines and increase skin firmness. Polypeptides also have functions such as antioxidant and anti-inflammatory effects, can resist the damage of environmental stress and free radicals, and make the skin healthier and brighter, etc., and are widely used in anti-aging products. For example, Chinese Patent Application 201510184638 provides a composition containing active peptides with an anti-fatigue effect; Chinese Patent Application 201510325301.6 contains a composite peptide and hesperidin methyl chalcone as an anti-aging eye essence; CN20161081808.2 discusses the cytokine-like effects of a nonapeptide composition; CN20161092671.3 relates to a composite product of elastin peptide and collagen peptide with a whitening effect; CN20161094437.9 is a whitening and freckle-removing skin care product containing ingredients such as anti-allergy repair peptides; CN20171115383.3 reports that a combination of a polypeptide and Zanthoxylum bungeanum fruit extract is an anti-aging composition.

[0004] However, although the prior art mentions the combination of polypeptides, it may only target a single target, such as only inhibiting muscle contraction like acetyl hexapeptide-8 or only promoting collagen production like palmitoyl pentapeptide-4. In addition, the problems of easy degradation and low transdermal efficiency of polypeptides have not been completely solved. For example, the disclosed patent CN117481992A achieves multi-target anti-aging by compounding multiple polypeptides, but its excipient system still relies on conventional moisturizers and emulsifiers, and does not solve the problems of polypeptide stability and permeability. Summary of the Invention

[0005] The purpose of the present invention is to overcome the above-mentioned deficiencies of the prior art, and provides a composite peptide composition, which realizes multi-target anti-aging by compounding multiple polypeptides, and achieves the effects of anti-aging, sensitive repair, and long-term moisturization; at the same time, in combination with a natural polysaccharide composite system, it breaks through the limitations of polypeptide stability and transdermal efficiency, and extends the active period of the product.

[0006] On the one hand, the present invention provides a composite peptide for anti-skin aging and skin repair, and the composite peptide comprises tripeptide-1, carnosine, palmitoyl tetrapeptide-7, palmitoyl pentapeptide-4, hexapeptide-9, acetyl hexapeptide-8, palmitoyl tripeptide-1, palmitoyl tripeptide-5, snake venom-like tripeptide, palmitoyl hexapeptide-12.

[0007] Preferably, the mass percentages of the components in the composite peptide are as follows: 0.5-3% tripeptide-1, 0.1-0.7% carnosine, 0.1-1.5% palmitoyl tetrapeptide-7, 0.05-0.4% palmitoyl pentapeptide-4, 0.05-0.4% hexapeptide-9, 0.5-4% acetyl hexapeptide-8, 0.2-2% palmitoyl tripeptide-1, 0.01-0.1% palmitoyl tripeptide-5, 0.01-0.2% snake venom-like tripeptide, 0.01-0.1% palmitoyl hexapeptide-12.

[0008] Preferably, the mass percentages of the components in the composite peptide are 2% tripeptide-1, 0.5% carnosine, 1% palmitoyl tetrapeptide-7, 0.2% palmitoyl pentapeptide-4, 0.2% hexapeptide-9, 3% acetyl hexapeptide-8, 1% palmitoyl tripeptide-1, 0.05% palmitoyl tripeptide-5, 0.1% snake venom-like tripeptide, 0.05% palmitoyl hexapeptide-12.

[0009] On the other hand, the present invention provides a composition containing the composite peptide. Specifically, the composition further comprises mannan, azone, and ferulic acid.

[0010] Further, the mass ratio of the composite peptide, mannan, azone, and ferulic acid is 1:0.2-0.6:0.1-0.5:0.05-0.1, and the rest is water.

[0011] Preferably, lecithin is added to the composition, and the composite peptide composition is encapsulated in lecithin.

[0012] Preferably, the mass ratio of the composite peptide to lecithin in the composition is 1:1-5.

[0013] In a preferred embodiment, the preparation method of the above-mentioned composite peptide composition is as follows: Step 1: Dissolve the prescribed amount of mannan in deionized water at 60°C and stir to form a homogeneous gel; Step 2: Dissolve the polypeptide in the gel solution obtained in Step 1, stir at a low speed at 40°C, add azone and ferulic acid, and adjust the pH value to 4-6 by dropwise adding acetic acid while stirring to form a composite carrier; Step 3: Slowly add the composite carrier obtained in Step 2 to the ethanol solution containing lecithin under stirring, mix well, evaporate to form a film at 40°C by rotary evaporation, and obtain the encapsulated polypeptide composition by hydration and high-pressure homogenization.

[0014] The application of the present invention combines a pharmaceutically effective amount of the polypeptide composition with a pharmaceutically acceptable excipient to prepare any pharmaceutically acceptable topical preparation.

[0015] Preferably, the topical preparation is one of a gel, essence, cream, lotion, facial cream, ointment, paste, lotion, patch, dressing, film-forming agent, cataplasm, aerosol or spray.

[0016] In a third aspect, the present invention provides an essence with anti-aging and wrinkle-removing effects, and the essence contains a polypeptide composition and a pharmaceutically acceptable excipient.

[0017] Preferably, the essence excipients include a humectant, a skin feel regulator, a thickening agent, a pH regulator, and a water solvent.

[0018] The humectant is preferably one or more of hyaluronic acid, glycerin, panthenol, and trehalose, and more preferably hyaluronic acid; its content is selected according to requirements and general knowledge in the art.

[0019] The skin feel regulator is selected from one or more of polydimethylsiloxane, nylon-12, octanoic acid, sodium polyacrylate, and polyglyceryl-10 dioleate.

[0020] The thickening agent is selected from one or a combination of xanthan gum, carbomer, hydroxyethyl cellulose, sodium polyacrylate, and polydimethylsiloxane.

[0021] The pH regulator is preferably citric acid and triethanolamine.

[0022] In a preferred embodiment, the present invention provides an essence of a polypeptide composition, and its component contents are as shown in Table 1 below: Table 1 Prescription of essence with 10% content of the review peptide composition

[0023] In a preferred embodiment, the present invention provides an essence of a review peptide composition, and its component contents are as shown in Table 2 below: Table 2 Prescription of essence with 15% content of the review peptide composition

[0024] Among them, the pH value range of the essence is 6 - 7.

[0025] On the other hand, the present invention provides a preparation method for an essence of a review peptide composition: (1) Add purified water, a humectant, and a skin feel regulator to the water phase pot, stir and heat up, and keep warm at 70°C for 1 h to obtain mixture A; add the thickening agent to the oil phase pot, stir and heat up, and keep warm at 65 - 75°C for 1 h to obtain mixture B; (2) After homogenizing mixture A and mixture B, lower the temperature, add the complex peptide composition to adjust the pH to 6 - 7, and stir to obtain the essence with anti - aging and wrinkle - removing effects.

[0026] Advantages of the present invention: Compared with the prior art, the combined peptides of the present application can better reduce the generation of expression lines from different pathways and achieve the purpose of rapid wrinkle removal: 1. The combination of four polypeptides, namely carnosine, palmitoyl tetrapeptide - 7, palmitoyl pentapeptide - 4, and hexapeptide - 9, with tripeptide - 1 has better permeability and can act on the epidermis, basal layer, and dermis of the skin respectively; at the same time, it promotes collagen I, III, VII and laminin - 5, and can cover more than 90% of the collagen; 2. Acetyl hexapeptide - 8, palmitoyl tripeptide - 1, palmitoyl tripeptide - 5, snake venom - like tripeptide, and palmitoyl hexapeptide - 12 polypeptides can well inhibit muscle contraction and promote collagen and elastin production, achieving the purpose of rapid wrinkle removal. Specific embodiments

[0027] The present invention will be further illustrated by the following examples. The test methods used in the following examples are all conventional methods unless otherwise specified; the materials, reagents, etc. used are all reagents and materials that can be obtained from commercial channels unless otherwise specified.

[0028] Example 1; Referring to the prescription in Table 3, heat and dissolve mannan in deionized water, and stir to form a homogeneous gel; dissolve the polypeptide in the gel solution, stir, add azone and ferulic acid, and dropwise add acetic acid to adjust the pH while stirring to form a composite carrier; slowly add the composite carrier to the lecithin ethanol solution under stirring, mix well, evaporate to form a film by rotary evaporation, and hydrate and then homogenize under high pressure to obtain the encapsulated polypeptide composition.

[0029] Table 3 Composition prescription of Example 1 (100 g)

[0030] The specific preparation process is as follows: Step 1: Dissolve the prescribed amount of mannan in deionized water at 60 °C and stir to form a homogeneous gel; Step 2: Dissolve the polypeptide in the gel solution of Step 1, stir at a low speed at 40 °C, add azone and ferulic acid, and dropwise add acetic acid to adjust the pH value to 4 - 6 to form a composite carrier; Step 3: Slowly add the composite carrier obtained in Step 2 to the lecithin ethanol solution under stirring, mix well, evaporate to form a film by rotary evaporation, and hydrate and then homogenize under high pressure to obtain the encapsulated polypeptide composition.

[0031] Example 2; Table 4 Composition Prescription of Example 2 (100 g)

[0032] The specific preparation process is as follows: Step 1: Dissolve the prescribed amount of mannan in deionized water at 60°C and stir to form a homogeneous gel; Step 2: Dissolve the polypeptide in the gel solution of Step 1, stir at a low speed at 40°C, add azone and ferulic acid, and adjust the pH value to 4 - 6 by dropwise adding acetic acid while stirring to form a composite carrier; Step 3: Slowly add the composite carrier obtained in Step 2 to the lecithin ethanol solution under stirring, mix well, rotate and evaporate to form a film, and after hydration, perform high-pressure homogenization to obtain the encapsulated polypeptide composition.

[0033] Example 3;

[0034] Table 5 Composition Prescription of Example 3 (100 g)

[0035] The specific preparation process is as follows: Step 1: Dissolve the prescribed amount of mannan in deionized water at 60°C and stir to form a homogeneous gel; Step 2: Dissolve the polypeptide in the gel solution of Step 1, stir at a low speed at 40°C, add azone and ferulic acid, and adjust the pH value to 4 - 6 by dropwise adding acetic acid while stirring to form a composite carrier; Step 3: Slowly add the composite carrier obtained in Step 2 to the lecithin ethanol solution under stirring, mix well, rotate and evaporate to form a film, and after hydration, perform high-pressure homogenization to obtain the encapsulated polypeptide composition.

[0036] Example 4;

[0037] Table 6 Composition Prescription of Example 4 (100 g)

[0038] The specific preparation process is as follows: Step 1: Dissolve the prescribed amount of mannan in deionized water at 60°C and stir to form a homogeneous gel; Step 2: Dissolve the polypeptide in the gel solution of Step 1, stir at a low speed at 40°C, add azone and ferulic acid, and adjust the pH value to 4 - 6 by dropwise adding acetic acid while stirring to form a composite carrier; Step 3: Slowly add the composite carrier obtained in Step 2 to the lecithin ethanol solution under stirring, mix well, rotate and evaporate to form a film, and after hydration, perform high-pressure homogenization to obtain the encapsulated polypeptide composition.

[0039] Comparative Example 1; Table 7 Composition Prescription of Comparative Example 1 (100 g)

[0040] The specific preparation process is as follows: Step 1: Dissolve the prescribed amount of mannan in deionized water at 60 °C and stir to form a homogeneous gel; Step 2: Dissolve the polypeptide in the gel solution obtained in Step 1, stir at a low speed at 40 °C, add azone and ferulic acid, and adjust the pH value to 4 - 6 by dropwise adding acetic acid while stirring to form a composite carrier; Step 3: Slowly add the composite carrier obtained in Step 2 to the lecithin ethanol solution under stirring, mix well, evaporate by rotation to form a film, and obtain the encapsulated polypeptide composition after hydration and high-pressure homogenization.

[0041] Comparative Example 2; Table 8 Composition Prescription of Comparative Example 2 (100 g)

[0042] The specific preparation process is as follows: Step 1: Dissolve the prescribed amount of mannan in deionized water at 60 °C and stir to form a homogeneous gel; Step 2: Dissolve the polypeptide in the gel solution obtained in Step 1, stir at a low speed at 40 °C, add azone and ferulic acid, and adjust the pH value to 4 - 6 by dropwise adding acetic acid while stirring to form a composite carrier; Step 3: Slowly add the composite carrier obtained in Step 2 to the lecithin ethanol solution under stirring, mix well, evaporate by rotation to form a film, and obtain the encapsulated polypeptide composition after hydration and high-pressure homogenization.

[0043] Comparative Example 3; Table 9 Composition Prescription of Comparative Example 3 (100 g)

[0044] The specific preparation process is as follows: Step 1: Dissolve the polypeptide in deionized water, stir at a low speed at 40 °C, add azone and ferulic acid, and adjust the pH value to 4 - 6 by dropwise adding acetic acid while stirring to form a composite carrier; Step 2: Slowly add the composite carrier obtained in Step 2 to the lecithin ethanol solution under stirring, mix well, evaporate by rotation to form a film, and obtain the encapsulated polypeptide composition after hydration and high-pressure homogenization.

[0045] Comparative Example 4; Table 10 Composition Prescription of Comparative Example 4 (100 g)

[0046] The specific preparation process is as follows: Step 1: Dissolve the prescribed amount of mannan in deionized water at 60 °C and stir to form a homogeneous gel; Step 2: Dissolve the polypeptide in the gel solution obtained in Step 1, stir at a low speed at 40 °C, add ferulic acid, and adjust the pH value to 4 - 6 by dropwise adding acetic acid while stirring to form a composite carrier; Step 3: Slowly add the composite carrier obtained in Step 2 to the lecithin ethanol solution under stirring, mix well, perform rotary evaporation to form a film, and obtain the encapsulated polypeptide composition by hydration and high-pressure homogenization.

[0047] Control Example 5; Table 11 Prescription of the Composition in Control Example 5 (100 g)

[0048] The specific preparation process is as follows: Step 1: Dissolve the prescribed amount of mannan in deionized water at 60 °C and stir to form a homogeneous gel; Step 2: Dissolve the polypeptide in the gel solution obtained in Step 1, stir at a low speed at 40 °C, add azone, and adjust the pH value to 4 - 6 by dropwise adding acetic acid while stirring to form a composite carrier; Step 3: Slowly add the composite carrier obtained in Step 2 to the lecithin ethanol solution under stirring, mix well, perform rotary evaporation to form a film, and obtain the encapsulated polypeptide composition by hydration and high-pressure homogenization.

[0049] Control Example 6; Table 12 Prescription of the Composition in Control Example 6 (100 g)

[0050] The specific preparation process is as follows: Step 1: Dissolve the prescribed amount of mannan in deionized water at 60 °C and stir to form a homogeneous gel; Step 2: Dissolve the polypeptide in the gel solution obtained in Step 1, stir at a low speed at 40 °C, add azone and ferulic acid, and adjust the pH value to 4 - 6 by dropwise adding acetic acid while stirring to form a composite carrier; Step 3: Slowly add the composite carrier obtained in Step 2 to the ethanol solution under stirring, mix well to obtain the polypeptide composition.

[0051] Control Example 7; Table 13 Prescription of the Composition in Control Example 7 (100 g)

[0052] The specific preparation process is as follows: Step 1: Dissolve the prescribed amount of mannan in deionized water at 60 °C and stir to form a homogeneous gel; Step 2: Dissolve the polypeptide in the gel solution of Step 1, stir at a low speed at 40°C, add azone and ferulic acid, and adjust the pH value to 4 - 6 by dropwise adding acetic acid while stirring to form a composite carrier; Step 3: Slowly add the composite carrier obtained in Step 2 to the lecithin ethanol solution under stirring, mix well, rotate and evaporate to form a film, and hydrate and then homogenize under high pressure to obtain the encapsulated polypeptide composition.

[0053] Comparative Example 8; Table 14 Prescription of the composition of Comparative Example 8 (100 g)

[0054] The specific preparation process is as follows: Step 1: Dissolve the prescribed amount of mannan in deionized water at 60°C and stir to form a homogeneous gel; Step 2: Dissolve the polypeptide in the gel solution of Step 1, stir at a low speed at 40°C, add azone and ferulic acid, and adjust the pH value to 4 - 6 by dropwise adding acetic acid while stirring to form a composite carrier; Step 3: Slowly add the composite carrier obtained in Step 2 to the lecithin ethanol solution under stirring, mix well, rotate and evaporate to form a film, and hydrate and then homogenize under high pressure to obtain the encapsulated polypeptide composition.

[0055] Verification Example: 1. Stability test; Test method: For the heat resistance test, place it at 45 ± 2°C for three months and observe the appearance, color and smell; for the cold resistance test, place it at -18 ± 2°C for three months and observe the appearance, color and smell of the cream; for the heat and cold cycle resistance test, store it at 45°C, room temperature and -18°C for 24 hours each, cycle 3 times, and observe the appearance, color and smell of the cream. The test results are shown in Table 15.

[0056] Table 15 Stability test results

[0057] As can be seen from Table 15, Examples 1 - 4 of the polypeptide composition of the present invention have excellent stability, and can ensure that the properties remain unchanged under high temperature, low temperature and high and low temperature cycle conditions. Among them, mannan, azone, ferulic acid and lecithin promote each other in terms of stability and have unexpected synergistic effects.

[0058] 2. In vivo skin permeability test; Thirty-six adult C57 mice were randomly divided into three groups of 12 mice each. A 10 mm × 5 mm area was shaved on the upper left back of the mice. 0.7 g of the composite peptide composition prepared by the methods described in Examples 1-4 and Comparative Examples 1-8 was evenly applied to the surface of this piece of skin (each mouse in each group was only applied with one component). After 8 h, the mice were sacrificed, and the applied area was gently wiped clean with a dry cloth. A skin sample was carefully cut from the applied area using a scalpel. First, the epidermal layer was peeled off to obtain an epidermal sample, and then the dermal layer was cut to obtain a dermal sample. The samples were chopped and placed in a centrifuge tube containing PBS buffer (pH 7.2). The samples were evenly suspended using an ultrasonic processor and shaken for 30 min to extract polypeptides. Filtered through gauze and then ultrafiltered using an ultrafiltration membrane (Millipore PLCC07610 5K). The concentration of the active ingredient polypeptide in the filtrate was determined by C18 HPLC (the mobile phase was acetonitrile containing 0.1% TFA and water containing 0.1% TFA). The average value of the three groups of data was taken, and the experimental results are as follows.

[0059] Table 16 Content of Composite Peptide in Mouse Skin

[0060] The above results show that the polypeptide composition of the present application can rapidly penetrate into the dermal layer, with significantly improved permeability and fast release rate; the absolute value of the polypeptide concentration in the dermal sample of Comparative Example 4 is much lower than the concentration of the epidermal samples of other samples. It shows that azone has the effect of promoting the transdermal rate of the dermal layer, and at the same time, the relative concentration of the polypeptides in Comparative Examples 1-6 is relatively low, indicating that mannan, azone, ferulic acid, and lecithin have a synergistic effect on promoting penetration.

[0061] 3. Anti-aging test; The compositions prepared in Examples 1-4 and Comparative Examples 1-8 were made into essence according to the following prescription for testing Table 17 Formula of Essence Containing Composite Peptide Composition

[0062] The specific process is as follows: (1) Add purified water, moisturizer, and skin feel regulator to the water phase pot, stir and heat up, and keep warm at 70 °C for 1 h to obtain mixture A; add thickener to the oil phase pot, stir and heat up, and keep warm at 65-75 °C for 1 h to obtain mixture B; (2) After homogenizing mixture A and mixture B, cool down, add the composite peptide composition to adjust the pH to 6-7, and stir to obtain the essence with anti-aging and anti-wrinkle effects.

[0063] Test method: Three hundred and sixty healthy women (aged 45 - 55) were selected and divided into 12 groups, with 30 people in each group. Each group used the facial creams in Examples 1 - 4 and Comparative Examples 1 - 8 respectively. The participants used the samples twice a day, morning and evening, for 56 days. During the study, the participants were not allowed to use other products. Before the start of the trial, the skin wrinkles and skin elasticity of the participants were measured; 7 days, 14 days, 28 days, and 56 days after using the facial cream products, the skin wrinkles and skin elasticity of the participants were measured again. Among them, the facial skin wrinkles were measured using a skin image analysis system (Skin Visiometer SV600); the skin elasticity was measured using a skin elasticity detector Cutometer® dual MPA580, and the average value of 30 in each group was calculated.

[0064] Wrinkle change rate (%) = (Average wrinkle value of each group after using the facial cream in the example / comparative example - Average wrinkle value of each group before use) / Average wrinkle value of each group before use * 100%. In the table, the wrinkle improvement rate is the positive value taken after calculating the change rate by the formula. Skin elasticity improvement rate (%) = (Average skin elasticity value of each group after using the facial cream in the example / comparative example - Average skin elasticity value of each group before use) / Average skin elasticity value of each group before use * 100%.

[0065] The specific test results are shown in Tables 18 and 19 below (the average value is taken after parallel experiments are carried out after the replacement of the essence auxiliary materials): Table 18 Facial wrinkle improvement rate

[0066] Table 19 Skin elasticity improvement rate

[0067] As can be seen from Table 18 and Table 19, when the essence is made from the compositions in Examples 1 - 4, an excellent instant anti - wrinkle effect can be achieved after using it once, and the longer the use time, the better the anti - wrinkle effect. At the same time, the combinations in Examples 1 - 4 can also achieve a good effect of improving skin elasticity. In addition, the anti - wrinkle and anti - aging properties of Examples 1 - 4 are significantly better than those of Comparative Examples 1 - 8, indicating that the polypeptide active ingredient, mannan, azone, ferulic acid, and lecithin in the anti - aging composition of the present invention reach synergy to best exert the anti - wrinkle and anti - aging effects of the active ingredient; in addition, when the content of the polypeptide active ingredient is optimized to a special ratio, the anti - wrinkle and anti - aging effects of the active ingredients can be maximally exerted. In summary, the composition described in the present invention has the effects of instant anti - wrinkle and long - term anti - aging.

[0068] It should be noted here that the embodiments of the present invention are only used to illustrate the present invention, rather than to limit the present invention. Therefore, any simple improvement to the present invention under the premise of the method of the present invention falls within the scope claimed by the present invention.

Claims

1. A composite peptide composition with anti-aging and anti-wrinkle effects, characterized in that, The composite peptide composition contains tripeptide-1, carnosine, palmitoyl tetrapeptide-7, palmitoyl pentapeptide-4, hexapeptide-9, acetyl hexapeptide-8, palmitoyl tripeptide-1, palmitoyl tripeptide-5, snake venom-like tripeptide, palmitoyl hexapeptide-12; further, mannan, azone and ferulic acid are added to the composition; the composition is encapsulated by lecithin.

2. The composite peptide composition with anti-aging and anti-wrinkle effects according to claim 1, characterized in that, In the composite peptide composition, the mass percentages of each component of the composite peptide are as follows: 0.5-3% tripeptide-1, 0.1-0.7% carnosine, 0.1-1.5% palmitoyl tetrapeptide-7, 0.05-0.4% palmitoyl pentapeptide-4, 0.05-0.4% hexapeptide-9, 0.5-4% acetyl hexapeptide-8, 0.2-2% palmitoyl tripeptide-1, 0.01-0.1% palmitoyl tripeptide-5, 0.01-0.2% snake venom-like tripeptide, 0.01-0.1% palmitoyl hexapeptide-12, 0.2%-5% mannan, 0.3%-5% azone, 0.2%-5% ferulic acid, 20%-40% lecithin, and the rest is water.

3. The composite peptide composition with anti-aging and anti-wrinkle effects according to claim 1, characterized in that, In the composite peptide, the mass percentages of each component of the composite peptide are 2% tripeptide-1, 0.5% carnosine, 1% palmitoyl tetrapeptide-7, 0.2% palmitoyl pentapeptide-4, 0.2% hexapeptide-9, 3% acetyl hexapeptide-8, 1% palmitoyl tripeptide-1, 0.05% palmitoyl tripeptide-5, 0.1% snake venom-like tripeptide, 0.05% palmitoyl hexapeptide-12, 3% mannan, 2% azone, 0.5% ferulic acid, 20% lecithin, and the rest is water.

4. A method for preparing the composite peptide composition with anti-aging and anti-wrinkle effects as claimed in claims 1-3, characterized in that, The preparation method of the composite peptide composition includes the following steps: Step 1: Dissolve the prescribed amount of mannan in deionized water at 60°C and stir to form a homogeneous gel. Step 2: Dissolve the polypeptide in the gel solution of Step 1, stir at low speed at 40°C, add azone and ferulic acid, and adjust the pH value to 4-6 by dropping acetic acid while stirring to form a composite carrier. Step 3: Slowly add the composite carrier obtained in Step 2 to the lecithin ethanol solution under stirring, mix well, rotate to evaporate to form a film, and hydrate and then homogenize under high pressure to obtain the encapsulated polypeptide composition.

5. Use of the composite peptide composition with anti-aging and anti-wrinkle effects according to claim 1 in cosmetics, characterized in that, The composition can be combined with pharmaceutically acceptable excipients to prepare any pharmaceutically acceptable external preparation; the external preparation is one of a gel, cream, lotion, facial cream, ointment, paste, lotion, patch, dressing, cataplasm, aerosol or spray.

6. Use of the peptide composition for verification according to claim 5 in cosmetics, characterized in that, The external preparation is the essence in the lotion.

7. An essence containing the peptide composition for rechecking described in claim 1, characterized in that, The essence also contains a humectant, a skin feel regulator, a thickener, a pH regulator, and a water solvent.

8. An essence containing the composite peptide composition according to claim 7, characterized in that, The humectant is preferably one or more of hyaluronic acid, glycerol, panthenol, trehalose; the skin feel regulator is selected from one or more of polydimethylsiloxane, nylon-12, octanoic acid, sodium polyacrylate, polyglycerol-10 dioleate; the thickener is selected from one or its combination of xanthan gum, carbomer, hydroxyethyl cellulose, sodium polyacrylate, polydimethylsiloxane; the pH regulator is citric acid and triethanolamine.

9. An essence containing the composite peptide composition according to claim 7, characterized in that, In the essence, the content percentages of each component are 10%-15% composite peptide composition, 3-10% humectant, 0.2-3% skin feel regulator, 0.05-0.3% thickener.

10. An essence containing the composite peptide composition according to claim 7, characterized in that, The preparation method of the essence includes the following steps: (1) Add purified water, a moisturizer, and a skin feel regulator to an aqueous phase pot, stir and heat up, and keep warm at 70 °C for 1 h to obtain mixture A; add a thickener to an oil phase pot, stir and heat up, and keep warm at 65-75 °C for 1 h to obtain mixture B; (2) After homogenizing mixture A and mixture B, cool down, add a compound peptide composition to adjust the pH to 6-7, and stir to obtain the essence with anti-aging and anti-wrinkle effects.

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