Application of strontium-rich water in inhibition of tyrosinase and application of strontium-rich water in cosmetics

Abstract

The invention belongs to the technical field of cosmetics, and provides application of strontium-rich water in inhibition of tyrosinase and application of strontium-rich water in cosmetics. When the strontium-rich water is applied to inhibition of tyrosinase, the content of strontium ions in the strontium-rich water is 0.2 mg / L to 2.0 mg / L. When the strontium-rich water is applied to cosmetics, the content of strontium ions in the strontium-rich water is 0.2 mg / L-2. 0mg / L. According to the invention, tyrosinase inhibition experiments and RT-PCR (Reverse Transcription-Polymerase Chain Reaction) experiments show that the strontium-rich water has a whitening effect, and the strontium-rich water can be used for beauty and skin care products, such as toners, emulsions, masks and the like, and plays roles of relieving, moisturizing and the like. The invention provides a wide prospect for the development and application of the strontium-rich ion water, and has important practical value.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and specifically relates to the application of strontium-rich water in inhibiting tyrosinase and its application in cosmetics. Background Art

[0002] Strontium is widely present in nature and human tissues. It is an essential trace element for the human body, and its chemical properties are similar to those of calcium. Strontium is mainly involved in bone development. 99% of the strontium in the human body exists in bones, and 0.7% exists in blood. In the medical field, in addition to promoting bone health, strontium can also prevent cardiovascular diseases, regulate reproductive health, prevent dental caries, reduce inflammation, have antioxidant effects, inhibit fat, etc. In addition, strontium also has other functions such as anti-cancer, anti-diabetes, promoting angiogenesis, and protecting the heart. However, there is no report on its efficacy in inhibiting the activity of tyrosinase and skin whitening.

[0003] Currently, there are four ways of whitening. The first is to brighten the skin gloss through antioxidant, such as vitamin C and its derivatives; the second is to inhibit melanin transport and improve sallow complexion, such as niacinamide; the third is to inhibit the activity of tyrosinase and reduce melanin synthesis, such as arbutin, kojic acid, and tranexamic acid; and inhibiting tyrosinase can also be divided into directly inhibiting tyrosinase or indirectly regulating the upstream target of tyrosinase to reduce the generation of tyrosinase; the fourth is to accelerate the metabolism of melanin, such as fruit acid and salicylic acid. There is no report on the specific way of directly inhibiting the activity of tyrosinase by strontium ions. Summary of the Invention

[0004] In view of the above skin whitening problem, on the one hand, the present invention provides an application of strontium-rich water in inhibiting tyrosinase, wherein the content of strontium ions in the strontium-rich water is 0.2 mg / L to 2.0 mg / L.

[0005] Preferably, the content of strontium ions in the strontium-rich water is 0.5 mg / mL to 2.0 mg / mL.

[0006] Preferably, strontium-rich water is the abbreviation of water rich in strontium ions, and can be natural strontium-rich water or strontium-rich water obtained by adding a strontium source to water.

[0007] Preferably, the strontium source is strontium oxide.

[0008] Preferably, the strontium source is a strontium salt. More preferably, the strontium salt is any one of strontium chloride, strontium bromide, strontium lactate, or strontium nitrate. In the present invention, strontium chloride is taken as an example for illustration, and other strontium salts not exemplified can achieve the same technical effects.

[0009] Strontium ion-rich water can significantly inhibit the expression of genes related to whitening, such as CAMP, mMITF, and mCREB, thereby inhibiting the activity of tyrosinase and reducing melanin production. It can be used to develop beauty and skincare products with whitening effects or as a means of adjuvant treatment for pigmented skin diseases. Therefore, in the second aspect of the present invention, there is also provided the application of the above-mentioned strontium-rich water in cosmetics. Among them, the content of strontium ions in the strontium-rich water is 0.2 mg / L to 2.0 mg / L. Preferably, the content of strontium ions in the strontium-rich water is 0.5 mg / mL to 2.0 mg / mL.

[0010] Preferably, strontium ions are applied to cosmetics, and skincare products with whitening, soothing, moisturizing and other effects are designed in combination with other skincare ingredients, including but not limited to products such as toner, lotion, and mask.

[0011] Preferably, 1000 g of toner contains the following components:

[0012] Strontium-rich water, 941 g;

[0013] Glycerol, 40 g;

[0014] EDTA disodium, 1 g;

[0015] PEG-40 hydrogenated castor oil, 10 g;

[0016] 1,2-Hexanediol, 5 g;

[0017] p-Hydroxyacetophenone, 3 g.

[0018] Preferably, the lotion, by weight, contains the following components:

[0019] Strontium-rich water, 91.43 parts;

[0020] Glycerol, 5 parts;

[0021] Sodium hyaluronate, 0.05 part;

[0022] EDTA disodium, 0.02 part;

[0023] Glycerol monostearate, 1.5 parts;

[0024] Polydimethylsiloxane, 1.0 part;

[0025] Aminomethylpropanol, 0.2 part;

[0026] 1,2-Hexanediol, 0.5 part;

[0027] p-Hydroxyacetophenone, 0.3 part.

[0028] Preferably, the preparation method of the above-mentioned natural strontium-rich water is specifically as follows: First, detect the metal ion content of spring water in a certain mountain area, deep groundwater in a certain place, etc., and select the water with a higher strontium ion content to determine a natural water source. The water from this specific natural water source is processed through processes such as filtration, disinfection, and concentration to ensure the safety of the water quality and that the strontium ion content meets the relevant national standards.

[0029] Preferably, the preparation method of adding a strontium source to water to obtain strontium-rich water is specifically as follows: On the premise of meeting the safety standards, add an appropriate amount of strontium source to pure water and stir to dissolve to prepare water rich in strontium ions. Preferably, the strontium source is selected from strontium oxide or strontium chloride. These two substances have good solubility in water and are relatively safe. Strontium oxide (SrO) reacts with water in water to form strontium hydroxide, and then releases strontium ions. Strontium chloride (SrCl2) directly ionizes into strontium ions and chloride ions in water. The strontium ion content is measured by strontium ion concentration.

[0030] The strontium ion-rich water provided by the present invention with a content of 0.2 mg / L to 2.0 mg / L has direct anti-inflammatory and whitening effects and is non-toxic. Its anti-inflammatory effect is to relieve skin inflammation by inhibiting the expression of the iNOS inflammatory factor; in addition, the strontium-rich water indirectly inhibits the activity of tyrosinase by inhibiting the expression of the whitening-related genes CAMP, mMITF, and mCREB, thereby playing a whitening role, which also shows its high safety. Compared with the prior art, it has at least the following beneficial effects:

[0031] 1. Beauty and skin care: It has the effect of soothing the skin and improving the skin condition.

[0032] 2. Whitening effect: Through tyrosinase inhibition experiments and cell experiments, it can be found that the strontium ion-rich water has a certain effect in whitening. Description of the Drawings

[0033] Figure 1 It is the relative expression quantity diagram of the anti-inflammatory related gene iNOS in Example 4 of the present invention;

[0034] Figure 2 It is the relative expression quantity diagram of the whitening related gene CAMP in Example 4 of the present invention;

[0035] Figure 3 It is the relative expression quantity diagram of the whitening related gene mCREB in Example 4 of the present invention;

[0036] Figure 4 It is the relative expression quantity diagram of the whitening related gene mMITF in Example 4 of the present invention. Detailed Embodiments

[0037] The technical solution of the present invention will be further described below in conjunction with the accompanying drawings and through specific embodiments. However, the following examples are only several examples of the present invention and do not represent or limit the scope of the protection of the present invention. The scope of protection of the present invention shall be subject to the claims.

[0038] Unless otherwise specified, the raw materials and materials used in the embodiments of the present invention are purchased through general commercial channels. In addition, in the present invention, unless otherwise specified, the comparison product is pure water.

[0039] The source information of the relevant raw materials, materials, and instruments involved in the following examples or comparative examples is as follows:

[0040] Pure water, brand: Wahaha;

[0041] Natural strontium-rich water, from Pingbian area;

[0042] L-Tyrosine, purchased from Yuanye Biotechnology Co., Ltd., model number: S20087;

[0043] Tyrosinase, purchased from Yuanye Biotechnology Co., Ltd., model number: S10171;

[0044] Dexamethasone sodium phosphate, purchased from Yuanye Biotechnology Co., Ltd., model number: D129705;

[0045] α-Arbutin, purchased from Yuanye Biotechnology Co., Ltd., model number: B24423;

[0046] Fetal bovine serum (FBS), EvaCell, product number: E01011;

[0047] Pen Strep, purchased from Gibco, product number: 15140-122;

[0048] PBS buffer (phosphate buffer solution, pH = 7.2 - 7.4), Beijing Solarbio Science & Technology Co., Ltd., product number: P1020;

[0049] RAW264.7 cells, mouse mononuclear macrophage leukemia cells, purchased from Suzhou Haixing Bio;

[0050] B16F10 cells, mouse skin melanoma cells, purchased from Suzhou Haixing Bio;

[0051] DMEM medium, purchased from Gibco, product number: 11995065;

[0052] Thiazolyl blue solution (MTT solution), purchased from Yuanye Biotechnology Co., Ltd., product number: R20227;

[0053] Filter paper, purchased from Beijing Solarbio Science & Technology Co., Ltd., product number: YA0162;

[0054] A small tangential flow ultrafiltration system device (20μm), purchased from Beijing Holes Biotechnology Co., Ltd., with the model number Mini T01;

[0055] An enzyme-linked immunosorbent assay instrument, purchased from Molecular Devices (Shanghai) Co., Ltd., with the model number SpectraMax i3x.

[0056] <Preparation Example 1>

[0057] This preparation example prepares a natural strontium-rich water.

[0058] The water from the selected natural water source was naturally precipitated for 24 hours. First, filter paper was used to remove larger particles, and then a small tangential flow ultrafiltration system device (20μm) was used to further remove tiny particles. The obtained water was concentrated using a rotary evaporator, and the concentration ratio was 25%, that is, 75% of the water was evaporated to obtain Sample 1. After testing, the strontium ion content was approximately 2.0 mg / L.

[0059] <Preparation Example 2>

[0060] This preparation example prepares a natural strontium-rich water.

[0061] The operation method was the same as that in Example 1, and the concentration ratio was 50%, that is, 50% of the water was evaporated to obtain Sample 2. After testing, the strontium ion content was approximately 1.0 mg / L.

[0062] <Preparation Example 3>

[0063] This preparation example prepares a natural strontium-rich water.

[0064] The operation method was the same as that in Example 1, and the concentration ratio was 90%, that is, 10% of the water was evaporated to obtain Sample 3. After testing, the strontium ion content was approximately 0.5 mg / L.

[0065] <Preparation Example 4>

[0066] This preparation example prepares an artificial strontium-rich water.

[0067] 0.29 mg of strontium oxide was added to 1 L of pure water to prepare a strontium-rich water with a concentration of 0.25 mg / L (calculated based on strontium ion concentration) to obtain Sample 4.

[0068] <Preparation Example 5>

[0069] This preparation example prepares an artificial strontium-rich water.

[0070] 0.59 mg of strontium oxide was added to 1 L of pure water to prepare a strontium-rich water with a concentration of 0.5 mg / L (calculated based on strontium ion concentration) to obtain Sample 5.

[0071] <Preparation Example 6>

[0072] This preparation example prepares an artificial strontium-rich water.

[0073] Add 1.19 mg of strontium chloride to 1 L of pure water to prepare a strontium-rich water with a concentration of 1 mg / L (calculated based on strontium ion concentration), obtaining Sample 6.

[0074] <Example 1>

[0075] In this example, the strontium ion content of the natural strontium-rich waters prepared in Preparation Examples 1 to 3 is determined.

[0076] According to GB / T 5750.6-2023 "Standard Test Methods for Drinking Water - Part 6: Metals and Metalloids", the strontium ion content of the prepared water samples (Samples 1 - 3) and pure water (control) is detected by inductively coupled plasma mass spectrometry method. The measurement results are shown in Table 1. Among them, the strontium ion content of Preparation Examples 4 - 6 in Table 1 is obtained by calculation.

[0077] Table 1. Strontium ion content

[0078]

[0079]

[0080] <Example 2>

[0081] In this example, the tyrosinase inhibitory effect of the water samples prepared in Preparation Examples 1 to 6 and the control is determined. Using L-tyrosine as the substrate, it is divided into an experimental group, a blank group, and a positive control group.

[0082] Experimental group: Add 100 μL (0.5 mg / mL) of L-tyrosine, 50 μL (200 U / mL) of tyrosinase, and 50 μL (5 mg / mL) of the water samples prepared in Preparation Examples 1 - 6 and pure water (control) to the 96-well plate in sequence;

[0083] Blank group: Add 100 μL (0.5 mg / mL) of L-tyrosine, 50 μL (200 U / mL) of tyrosinase, and 50 μL of PBS buffer to the 96-well plate in sequence;

[0084] Control group: Add 100 μL (0.5 mg / mL) of L-tyrosine and 100 μL of PBS buffer to the 96-well plate in sequence;

[0085] Positive control group: Add 100 μL (0.5 mg / mL) of L-tyrosine, 50 μL (200 U / mL) of tyrosinase, and 50 μL (5 mg / mL) of an aqueous solution of α-arbutin to the 96-well plate in sequence.

[0086] After reacting in the dark for 30 min, place it in an enzyme-linked immunosorbent assay (ELISA) reader and measure the absorbance value at 475 nm. Calculate the tyrosinase inhibition rate. The calculation formula is as follows: The results are shown in Table 2.

[0087] Table 2. Tyrosinase inhibition rate

[0088]

[0089]

[0090] It can be seen from the results in the table that when the content of strontium ions is in the range of 0.5 mg / L to 2.0 mg / L, the inhibition rate of tyrosinase is about 15%. The inhibition rate of strontium-rich water with a concentration of 0.25 mg / L on tyrosinase is only 5%, while pure water has no inhibitory effect. This experiment proves that strontium ions have the effect of inhibiting tyrosinase to produce a whitening effect, and there is a dose-effect relationship.

[0091] <Example 3>

[0092] In this example, the cytotoxicity of the water samples prepared in Preparation Examples 1 to 6 and the reference samples was measured.

[0093] Take RAW264.7 cells and B16F10 cells in good growth state. After digestion, add them to DMEM medium containing 10% fetal bovine serum (FBS) and 1% Pen Strep to make a cell suspension. Then inoculate the cells into a 96-well plate at a concentration of 5×10 4 cells / mL, and culture them in an incubator at 37 °C and 5% CO2 for 24 h. Then add the water sample solutions prepared in Preparation Examples 1 to 6 to the cell culture medium (DMEM medium + 10% FBS + 1% Pen Strep). The concentration of the water sample solution is 200 μM. The preparation method is as follows: Add the water samples prepared in Preparation Examples 1 to 6 to the complete medium (DMEM medium + 10% FBS + 1% Pen Strep) at a volume ratio of 25% respectively to prepare 6 kinds of water sample solutions. At the same time, set a control group (replace the water sample with the complete medium) and a blank group (only the complete medium), and set 3 replicates for each group. Continue to culture under the conditions of 37 °C and 5% CO2 for 24 h, then add 25 μL of MTT and continue to culture for 2 h to 4 h. Wash away the culture medium, add 150 μL / well of dimethyl sulfoxide (DMSO), shake for 10 min, and measure the absorbance value at a wavelength of 570 nm with an enzyme-linked immunosorbent assay (ELISA) reader. According to the formula Calculate the cell survival rate. The results are shown in Table 3.

[0094] Table 3. Cell survival rate

[0095] Group RAW264.7 cell viability (%) B16F10 cell viability (%) Preparation Example 1 123％ 123％ Preparation Example 2 112％ 124％ Preparation Example 3 101％ 112％ Preparation Example 4 106％ 108％ Preparation Example 5 114％ 103％ Preparation Example 6 109％ 103％ Control 98％ 101％

[0096] As can be seen from the results in Table 3, when the strontium ion content is in the range of 0.2 mg / L to 2.0 mg / L, it has no effect on the survival rates of the two types of cells. Thus, it can be known that the strontium-rich water has relatively high safety.

[0097] <Example 4>

[0098] In this example, gene expression assays were performed on the water samples prepared in Preparation Examples 1 to 6 and the reference samples for whitening and anti-inflammatory related genes.

[0099] The specific operation is as follows: The water samples of Preparation Examples 1 to 6 and purified water (reference sample) were added to the complete medium (DMEM medium + 10% FBS + 1% Pen Strep) at a volume ratio of 25%. After culturing for 24 hours, under low-temperature conditions, a total RNA extraction kit was used to extract total RNA. The cDNA reverse transcription synthesis operation was carried out in accordance with the instructions of the reverse transcription kit. The real-time PCR formal experiment was carried out under the optimal conditions, and the relative expression quantification of four genes related to whitening and anti-inflammatory was carried out using the 2 -ΔΔCt calculation method. These four genes are iNOS, CAMP, mCREB, and mMITF respectively. In order to ensure the success of the experiment, dexamethasone sodium phosphate and α-arbutin were selected as the positive control groups of the experiment. The results are shown in Figures 1 to 4 . Among them, Figures 1 to 4 the dexamethasone in refers to dexamethasone sodium phosphate; Figures 1 to 4 in represents that there is a very significant difference between the blank group and the model group, that is, p < 0.01; ** represents that there is a great significance between the model group and the experimental group, that is, p < 0.05; *** represents that there is a very significant difference between the model group and the experimental group, that is, p < 0.01; ns represents that there is no significant difference between the model group and the experimental group.

[0100] From Figure 1 it can be seen that the model group uses lipopolysaccharide as an inducer, and there is a significant difference between this model group and the blank group, indicating that the inflammation model is successfully established; dexamethasone as a positive reference sample also has a significant anti-inflammatory effect. Compared with the model group, Preparation Examples 1 to 3 and Preparation Examples 5 to 6 can significantly reduce the relative gene expression level of the anti-inflammatory factor iNOS, indicating that the strontium-rich water has a certain anti-inflammatory effect. At the same time, from Figure 1 it can be seen that the anti-inflammatory effects of natural strontium-rich water and artificial strontium-rich water are basically the same at similar strontium ion concentrations. However, due to the low concentration of Preparation Example 4, it cannot significantly reduce the relative gene expression level of the anti-inflammatory factor iNOS, indicating that the anti-inflammatory effect of a strontium ion content of 0.25 mg / L is not good.

[0101] From Figure 2It can be seen that α - arbutin, as a positive control, has a significant whitening effect, which also verifies the stability of the cell model. Compared with the blank group, Preparation Examples 1 - 3 and Preparation Examples 5 - 6 can significantly reduce the relative gene expression level of the whitening - related gene CAMP, indicating that strontium - rich water has a certain whitening effect. At the same time, from Figure 2 It can be seen that the whitening effects of natural strontium - rich water and artificial strontium - rich water are basically the same at similar strontium ion concentrations. However, due to the low concentration in Preparation Example 4, it cannot significantly reduce the relative gene expression level of CAMP, indicating that the whitening effect is not good at a strontium ion content of 0.25 mg / L.

[0102] From Figure 3 It can be seen that α - arbutin, as a positive control, has a significant whitening effect, which also verifies the stability of the cell model. Compared with the blank group, Preparation Examples 1 - 3 and Preparation Examples 5 - 6 can significantly reduce the relative gene expression level of the whitening - related gene mCREB, indicating that strontium - rich water has a certain whitening effect. At the same time, from Figure 3 It can be seen that the whitening effects of natural strontium - rich water and artificial strontium - rich water are basically the same at similar strontium ion concentrations. However, due to the low concentration in Preparation Example 4, it cannot significantly reduce the relative gene expression level of mCREB, indicating that the whitening effect is not good at a strontium ion content of 0.25 mg / L.

[0103] From Figure 4 It can be seen that α - arbutin, as a positive control, has a significant whitening effect, which also verifies the stability of the cell model. Compared with the blank group, Preparation Examples 1 - 3 and Preparation Examples 5 - 6 can significantly reduce the relative gene expression level of the whitening - related gene mMITF, indicating that strontium - rich water has a certain whitening effect. At the same time, from Figure 4 It can be seen that the anti - inflammatory effects of natural strontium - rich water and artificial strontium - rich water are basically the same at similar strontium ion concentrations. However, due to the low concentration in Preparation Example 4, it cannot significantly reduce the relative gene expression level of mMITF, indicating that the whitening effect is not good at a strontium ion content of 0.25 mg / L.

[0104] From the above gene expression results combined with the tyrosinase inhibition rate results, it can be seen that whether it is natural strontium - rich water or artificial strontium - rich water, when the strontium content is in the range of 0.5 - 2.0 mg / L, the expression levels of the four genes iNOS, CAMP, mCREB, and mMITF will be reduced. It shows that there are three whitening mechanisms of strontium - rich water. First, it can play an anti - inflammatory role by inhibiting the expression of anti - inflammatory factors to soothe the skin; second, it can inhibit the activity of tyrosinase to reduce melanin production; finally, it can regulate the whitening - related genes to inhibit the expression of the TYR gene to regulate the activity of tyrosinase and inhibit melanin production. Since its mechanism of action is not a direct effect, it also indicates its high safety.

[0105] <Example 5>

[0106] In this example, a moisturizing spray is prepared by using the strontium-rich water of Preparation Example 1 instead of water. The component dosages and preparation method are as follows:

[0107] The dosages of each component are as follows: 941 g of strontium-rich water, 40 g of glycerol, 1 g of disodium EDTA, 10 g of PEG-40 hydrogenated castor oil, 5 g of 1,2-hexanediol, and 3 g of p-hydroxyacetophenone.

[0108] Step 1: Accurately measure the strontium-rich water, glycerol, 1,2-hexanediol, and p-hydroxyacetophenone according to the above dosages, and accurately weigh disodium EDTA. Add them together to a stirring pot and mix and stir at 60 °C and a speed of 500 r / min to 800 r / min for 30 min to 60 min until evenly dispersed;

[0109] Step 2: Accurately measure PEG-40 hydrogenated castor oil according to the above dosage and add it to the system in Step 1. Stir at a speed of 500 r / min to 800 r / min for 3 min to 5 min, stir evenly and discharge. Then, 1000 ml of moisturizing spray can be prepared. Divide it into 20 portions of 50 ml each to obtain the finished product.

[0110] <Example 6>

[0111] In this example, a 500 g emulsion is prepared using the strontium-rich water of Preparation Example 1 as the base material. The formula is shown in Table 4 below.

[0112] Table 4. Emulsion formula

[0113]

[0114] The preparation method is as follows:

[0115] Step 1: Add the raw materials in Group A to a 500 mL beaker in sequence, heat to 80 °C, and homogenize and stir until dissolved evenly;

[0116] Step 2: Add the raw materials in Group B to the beaker in Step 1, then heat to 80 °C to dissolve and disperse evenly, and cool down to 60 °C;

[0117] Step 3: Add the raw materials in Group C to the beaker in Step 2 and stir evenly;

[0118] Step 4: Finally, add the raw materials in Group D to the beaker and stir evenly, and cool down to below 37 °C to obtain the emulsion.

[0119] <Comparative Example 1>

[0120] In this example, an emulsion is prepared using purified water as the base material. The formula is shown in Table 4, and only the strontium-rich water is replaced with purified water.

[0121] <Example 7>

[0122] In this example, the tyrosinase inhibitory effects of the emulsions prepared in Example 6 and Comparative Example 1 were measured. Using L-tyrosine as the substrate, it was divided into an experimental group, a blank group, and a positive control group.

[0123] Experimental group: 100 μL (0.5 mg / mL) of L-tyrosine, 50 μL (200 U / mL) of tyrosinase, and 50 μL (5 mg / mL) of the emulsions prepared in Example 6 and Comparative Example 1 were successively added to a 96-well plate.

[0124] Blank group: 100 μL (0.5 mg / mL) of L-tyrosine, 50 μL (200 U / mL) of tyrosinase, and 50 μL of PBS buffer were successively added to a 96-well plate.

[0125] Control group: 100 μL (0.5 mg / mL) of L-tyrosine and 100 μL of PBS buffer were successively added to a 96-well plate.

[0126] Positive control group: 100 μL (0.5 mg / mL) of L-tyrosine, 50 μL (200 U / mL) of tyrosinase, and 50 μL (5 mg / mL) of an aqueous solution of α-arbutin were successively added to a 96-well plate.

[0127] After reacting in the dark for 30 min, it was placed in an enzyme-linked immunosorbent assay (ELISA) reader, and the absorbance value was measured at 475 nm. The tyrosinase inhibition rate was calculated, and the calculation formula was: The results are shown in Table 5.

[0128] Table 5. Tyrosinase inhibition rate

[0129]

[0130] It can be seen from the results in the table that the inhibition rate of the skin care products prepared with strontium-rich water on tyrosinase is about 10%, while Comparative Example 1 has no inhibitory effect, indicating that strontium-rich water has a certain whitening effect.

[0131] The applicant declares that the above is only the specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Those skilled in the art should understand that any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed by the present invention fall within the protection scope and the disclosure scope of the present invention.

Claims

1. Use of strontium-rich water in inhibiting tyrosinase, characterized in that The content of strontium ions in the strontium-rich water is 0.2 mg / L to 2.0 mg / L.

2. Use of strontium-rich water as claimed in claim 1 for inhibiting tyrosinase, characterized in that, The content of the strontium ions in the strontium-rich water is 0.5 mg / L to 2.0 mg / L.

3. Use of strontium-rich water as claimed in claim 1 for inhibiting tyrosinase, characterized in that, The strontium-rich water is natural strontium-rich water or strontium-rich water obtained by adding strontium oxide or strontium salt to water.

4. Use of strontium-rich water as claimed in claim 3 in inhibiting tyrosinase, characterized in that, The strontium salt is any one of strontium chloride, strontium bromide, strontium lactate or strontium nitrate.

5. The application of strontium-rich water as claimed in claim 3 in inhibiting tyrosinase, characterized in that, The natural strontium-rich water is obtained by processing natural water through a small tangential flow ultrafiltration system equipment and then concentrating it.

6. Application of strontium-rich water in cosmetics, characterized in that, The content of strontium ions in the strontium-rich water is 0.2 mg / L to 2.0 mg / L.

7. The application of the strontium-rich water as described in claim 6 in cosmetics, characterized in that, The content of the strontium ions in the strontium-rich water is 0.5 mg / L to 2.0 mg / L.

8. The application of strontium-rich water in cosmetics according to claim 6, characterized in that, The strontium-rich water is natural strontium-rich water or strontium-rich water obtained by adding strontium oxide or strontium salt to water; the cosmetic is any one of toner, emulsion or facial mask.

9. The application of strontium-rich water in cosmetics according to claim 8, characterized in that, 1000 g of the toner comprises the following components: Strontium-rich water, 941 g; Glycerol, 40 g; Disodium EDTA, 1 g; PEG-40 hydrogenated castor oil, 10 g; 1,2-Hexanediol, 5 g; p-Hydroxyacetophenone, 3 g.

10. The application of strontium-rich water in cosmetics according to claim 8, wherein The emulsion, by weight, comprises the following components: Strontium-rich water, 91.43 parts; Glycerol, 5 parts; Sodium hyaluronate, 0.05 part; Disodium EDTA, 0.02 part; Glyceryl stearate, 1.5 parts; Polydimethylsiloxane, 1.0 part; Aminomethylpropanol, 0.2 part; 1,2-Hexanediol, 0.5 part; p-Hydroxyacetophenone, 0.3 part.

Images