Composition containing fucus extract and application of composition in preparation of product with sunscreen effect

Abstract

The invention relates to the technical field of natural medicinal chemistry, and particularly discloses a fucus extract-containing composition, which comprises a fucus extract and a gentiana macrophylla extract, wherein the weight ratio of the fucus extract to the gentiana macrophylla extract is (1-10): (1-10). Researches show that the composition obtained by combining the fucus extract extracted by using acetone and the gentiana macrophylla extract extracted by using ethanol has a better sunscreen effect; the sunscreen effect of the extract is obviously higher than that of the fucus extract independently extracted by acetone and that of the gentiana macrophylla extract independently extracted by ethanol. Therefore, the composition containing the fucus extract, which is disclosed by the invention, has important application value when being used as an active ingredient for preparing a product with a sunscreen effect.

Description

Technical Field

[0001] The present invention relates to the technical fields of natural product chemistry and daily necessities, and particularly relates to a composition containing fucus extract and its application in the preparation of products with sunscreen effects. Background Art

[0002] With the improvement of people's living standards and the increasing attention to skin health, the demand for sunscreen products is on the rise. Ultraviolet (UV) radiation is one of the important factors leading to skin sunburn, aging and cancer. Therefore, the development of highly efficient and safe sunscreen products has become an important research direction in the cosmetics industry.

[0003] Common sunscreen agents on the current market are mainly divided into two categories: chemical sunscreen agents and physical sunscreen agents. Chemical sunscreen agents achieve the sunscreen effect by absorbing ultraviolet rays and converting them into heat energy for release, such as avobenzone, octocrylene, etc.; physical sunscreen agents form a protective film on the skin surface to reflect or scatter ultraviolet rays, such as titanium dioxide and zinc oxide. However, these traditional sunscreen agents have some limitations. For example, some chemical sunscreen agents may cause skin allergic reactions. Although physical sunscreen agents are relatively mild, they will make the skin appear white after use, affecting the aesthetics, and their sunscreen effect is easily affected by factors such as sweat.

[0004] In view of the above problems, natural plant extracts have received extensive attention due to their good biological activity and safety, and have become a new hot spot in the research and development of sunscreen products. Therefore, the development of a composition containing natural extracts with sunscreen activity has important application value. Summary of the Invention

[0005] In order to overcome at least one of the technical problems existing in the prior art, the present invention first provides a composition containing fucus extract and its application in the preparation of products with sunscreen effects.

[0006] The above technical problems to be solved by the present invention are realized through the following technical solutions: The present invention first provides a composition containing fucus extract, which contains fucus extract and gentiana macrophylla pall extract; Among them, the weight ratio of the fucus extract to the gentiana macrophylla pall extract is 1-10:1-10.

[0007] The present invention provides a completely new composition; the inventor surprisingly found in the research that the composition obtained by combining the fucus extract extracted with acetone and the gentiana macrophylla pall extract extracted with ethanol has a good sunscreen effect; its sunscreen effect is significantly higher than that of the fucus extract extracted with acetone alone and the gentiana macrophylla pall extract extracted with ethanol alone. ​

[0008] Preferably, the weight ratio of the fucus extract and gentiana macrophylla pall extract is 1-5:1-5.

[0009] More preferably, the weight ratio of the fucus extract and gentiana macrophylla pall extract is 1-3:1-3.

[0010] Most preferably, the weight ratio of the fucus extract and gentiana macrophylla pall extract is 1:1.

[0011] Preferably, the fucus extract is prepared by the following method: Take fucus, extract it with an organic solvent, take the extract after extraction, and concentrate and dry the extract to obtain the fucus organic solvent extract; take the fucus organic solvent extract to obtain the fucus extract.

[0012] Preferably, the material-liquid ratio of fucus to the organic solvent is 1 kg:5-15 L.

[0013] Most preferably, the material-liquid ratio of fucus to the organic solvent is 1 kg:8 L.

[0014] Preferably, the organic solvent is acetone.

[0015] Preferably, the extraction is heat reflux extraction, and the extraction time is 1-2 h.

[0016] Preferably, the preparation method of the fucus extract further comprises the following steps: Load the fucus organic solvent extract onto a macroporous resin column, first elute with an ethanol aqueous solution with a volume fraction of 11-13% to remove impurities; then elute with an ethanol aqueous solution with a volume fraction of 27-29%, collect the eluate eluted by the ethanol aqueous solution with a volume fraction of 27-29%, concentrate and dry it to obtain the fucus macroporous resin elution fraction; take the fucus macroporous resin elution fraction to obtain the fucus extract.

[0017] Most preferably, the preparation method of the fucus extract further comprises the following steps: Load the fucus organic solvent extract onto a macroporous resin column, first elute with an ethanol aqueous solution with a volume fraction of 12% to remove impurities; then elute with an ethanol aqueous solution with a volume fraction of 28%, collect the eluate eluted by the ethanol aqueous solution with a volume fraction of 28%, concentrate and dry it to obtain the fucus macroporous resin elution fraction; take the fucus macroporous resin elution fraction to obtain the fucus extract.

[0018] Preferably, the macroporous resin column is a D101 type macroporous resin column.

[0019] In further research, the inventors found that the combination of the Fucus extract obtained by eluting the organic solvent extract of Fucus obtained by extraction with acetone through the above-mentioned macroporous resin elution conditions of the present invention and the Gentiana macrophylla extract has a further significant improvement in its sunscreen effect compared to the combination of the Fucus extract obtained by extraction with acetone and the Gentiana macrophylla extract; it has an extremely excellent sunscreen effect.

[0020] The inventors also found in the research that the macroporous resin elution conditions of the present invention are very crucial; only when the combination of the Fucus extract obtained by eluting the organic solvent extract of Fucus obtained by extraction with acetone through the above-mentioned macroporous resin elution conditions of the present invention and the Gentiana macrophylla extract can the sunscreen effect be further significantly improved; however, when the combination of the Fucus extract obtained by eluting the organic solvent extract of Fucus obtained by extraction with acetone through other macroporous resin elution conditions and the Gentiana macrophylla extract, the sunscreen effect cannot be further significantly improved.

[0021] Preferably, the Gentiana macrophylla extract is prepared by the following method: Take Gentiana macrophylla, extract it with an organic solvent, take the extract after the extraction is completed, and concentrate and dry the extract to obtain the organic solvent extract of Gentiana macrophylla; take the organic solvent extract of Gentiana macrophylla to obtain the Gentiana macrophylla extract.

[0022] Preferably, the material-liquid ratio of Gentiana macrophylla to the organic solvent is 1 kg:5 - 15 L.

[0023] Most preferably, the material-liquid ratio of Gentiana macrophylla to the organic solvent is 1 kg:12 L.

[0024] Preferably, the organic solvent is ethanol.

[0025] Preferably, the ethanol is an ethanol aqueous solution with a volume fraction of 80%.

[0026] Preferably, the extraction is heating reflux extraction, and the extraction time is 1 - 2 h.

[0027] The present invention also provides the use of the above-mentioned composition containing Fucus extract in the preparation of a product with a sunscreen effect.

[0028] Preferably, the product is a sunscreen.

[0029] Beneficial effects: The present invention provides a novel composition containing Fucus vesiculosus extract; the composition is obtained by combining the Fucus vesiculosus extract obtained by extraction with acetone and the Gentiana macrophylla Pall. extract obtained by extraction with ethanol, and it has good sunscreen effect; its sunscreen effect is significantly higher than that of the Fucus vesiculosus extract obtained by extraction with acetone alone and the Gentiana macrophylla Pall. extract obtained by extraction with ethanol alone. Therefore, using the composition containing Fucus vesiculosus extract of the present invention as an active ingredient for preparing products with sunscreen effect has important application value. Detailed implementation manners

[0030] The following specific examples are used to further explain the present invention, but the examples do not limit the present invention in any form.

[0031] Example 1 Preparation of the composition containing Fucus vesiculosus extract Mix the Fucus vesiculosus extract and the Gentiana macrophylla Pall. extract evenly according to a weight ratio of 1:1 to obtain the composition containing Fucus vesiculosus extract; The Fucus vesiculosus extract is prepared by the following method: Take Fucus vesiculosus, heat it to boiling with an organic solvent and then carry out reflux extraction for 1 h. After the extraction is completed, take the extract, concentrate and dry the extract to obtain the Fucus vesiculosus organic solvent extract; take the Fucus vesiculosus organic solvent extract to obtain the Fucus vesiculosus extract; wherein, the material-liquid ratio of Fucus vesiculosus to the organic solvent is 1 kg:8 L; the organic solvent is acetone.

[0032] The Gentiana macrophylla Pall. extract is prepared by the following method: Take Gentiana macrophylla Pall., heat it to boiling with an organic solvent and then carry out reflux extraction for 1 h. After the extraction is completed, take the extract, concentrate and dry the extract to obtain the Gentiana macrophylla Pall. organic solvent extract; take the Gentiana macrophylla Pall. organic solvent extract to obtain the Gentiana macrophylla Pall. extract; wherein, the material-liquid ratio of Gentiana macrophylla Pall. to the organic solvent is 1 kg:12 L; the organic solvent is an ethanol aqueous solution with a volume fraction of 80%.

[0033] Example 2 Preparation of the composition containing Fucus vesiculosus extract Mix the Fucus vesiculosus extract and the Gentiana macrophylla Pall. extract evenly according to a weight ratio of 1:1 to obtain the composition containing Fucus vesiculosus extract; The preparation method of the Gentiana macrophylla Pall. extract is the same as that in Example 1; The Fucus vesiculosus extract is prepared by the following method: (1) Take Fucus vesiculosus, heat it to boiling with an organic solvent and then carry out reflux extraction for 1 h. After the extraction is completed, take the extract, concentrate and dry the extract to obtain the Fucus vesiculosus organic solvent extract; wherein, the material-liquid ratio of Fucus vesiculosus to the organic solvent is 1 kg:8 L; the organic solvent is acetone; (2) The organic solvent extract of Fucus vesiculosus was loaded onto a D101 macroporous resin column. First, it was eluted with 5 column volumes of an ethanol aqueous solution with a volume fraction of 12% to remove impurities. Then, it was eluted with 5 column volumes of an ethanol aqueous solution with a volume fraction of 28%. The eluate eluted with the ethanol aqueous solution with a volume fraction of 28% was collected, concentrated and dried to obtain the elution fraction of Fucus vesiculosus on the macroporous resin. The elution fraction of Fucus vesiculosus on the macroporous resin was taken to obtain the Fucus vesiculosus extract.

[0034] Comparative Example 1 Preparation of the composition containing Fucus vesiculosus extract The Fucus vesiculosus extract and the Gentiana macrophylla Pall. extract were mixed evenly at a weight ratio of 1:1 to obtain the composition containing Fucus vesiculosus extract. The preparation method of the Gentiana macrophylla Pall. extract was the same as that in Example 1. The Fucus vesiculosus extract was prepared by the following method: (1) Take Fucus vesiculosus, heat it to boiling with an organic solvent and then reflux for 1 h. After the extraction, take the extract, concentrate and dry the extract to obtain the organic solvent extract of Fucus vesiculosus. Among them, the material-liquid ratio of Fucus vesiculosus to the organic solvent is 1 kg:8 L. The organic solvent is acetone. (2) The organic solvent extract of Fucus vesiculosus was loaded onto a D101 macroporous resin column. First, it was eluted with 5 column volumes of an ethanol aqueous solution with a volume fraction of 5% to remove impurities. Then, it was eluted with 5 column volumes of an ethanol aqueous solution with a volume fraction of 15%. The eluate eluted with the ethanol aqueous solution with a volume fraction of 15% was collected, concentrated and dried to obtain the elution fraction of Fucus vesiculosus on the macroporous resin. The elution fraction of Fucus vesiculosus on the macroporous resin was taken to obtain the Fucus vesiculosus extract.

[0035] Comparative Example 2 Preparation of the composition containing Fucus vesiculosus extract The Fucus vesiculosus extract and the Gentiana macrophylla Pall. extract were mixed evenly at a weight ratio of 1:1 to obtain the composition containing Fucus vesiculosus extract. The preparation method of the Gentiana macrophylla Pall. extract was the same as that in Example 1. The Fucus vesiculosus extract was prepared by the following method: (1) Take Fucus vesiculosus, heat it to boiling with an organic solvent and then reflux for 1 h. After the extraction, take the extract, concentrate and dry the extract to obtain the organic solvent extract of Fucus vesiculosus. Among them, the material-liquid ratio of Fucus vesiculosus to the organic solvent is 1 kg:8 L. The organic solvent is acetone. (2) Load the organic solvent extract of Fucus vesiculosus onto a D101 macroporous resin column. First, elute with 5 column volumes of an aqueous ethanol solution with a volume fraction of 30% to remove impurities. Then, elute with 5 column volumes of an aqueous ethanol solution with a volume fraction of 60%, collect the eluate eluted by the aqueous ethanol solution with a volume fraction of 60%, concentrate and dry it to obtain the elution fraction of Fucus vesiculosus on the macroporous resin. Take the elution fraction of Fucus vesiculosus on the macroporous resin to obtain the Fucus vesiculosus extract as described above.

[0036] Experimental Example 1 Sunscreen Test Add HaCat cells at 1x10 4 cells / well to a 96-well plate, add 100 μL of DMEM medium to each well and culture for 24 h, then discard the medium. Then, add 100 μL of DMEM medium containing 1 mg / mL of the test composition to the experimental group and continue to culture for 24 h. Then, irradiate the experimental group with ultraviolet light at 100 mJ / cm 2 for 20 min. After that, add MTT and incubate for 4 h, then discard the supernatant, add DMSO, and measure the absorbance value (490 nm) with an enzyme-linked immunosorbent assay (ELISA) reader after standing in the dark for 15 min. In addition, set up a control group without adding the test composition and without ultraviolet irradiation and a blank group without adding cells and the test composition according to the above method. Calculate the cell survival rate according to the absorbance value, and the results are shown in Table 1.

[0037] Cell survival rate (%) = [(As - Ab) / (Ac - Ab)]×100%; where As represents the absorbance value of the experimental group, Ac represents the absorbance value of the control group; Ab represents the absorbance value of the blank group.

[0038] Among them, Experimental Groups 1-6 respectively test the compositions prepared in Examples 1 and 2, the Fucus vesiculosus extract and Gentiana macrophylla Pall. extract prepared according to the method of Example 1, and the compositions prepared in Comparative Examples 1 and 2.

[0039] Table 1. Experimental results of cell survival rate

[0040] It can be seen from the experimental results in Table 1 that the cell survival rate of Experimental Group 1 is significantly higher than that of Experimental Groups 3 and 4. This shows that: the composition obtained by combining the Fucus vesiculosus extract extracted with acetone and the Gentiana macrophylla Pall. extract extracted with ethanol has a good sunscreen effect; its sunscreen effect is significantly higher than that of the Fucus vesiculosus extract extracted with acetone alone and the Gentiana macrophylla Pall. extract extracted with ethanol alone. Combining the Fucus vesiculosus extract extracted with acetone and the Gentiana macrophylla Pall. extract extracted with ethanol can produce a synergistic sunscreen effect.

[0041] As can be seen from the experimental results in Table 1, the cell survival rate of experimental group 2 is further significantly higher than that of experimental group 1; this indicates that: the combination of the Fucus extract obtained by further eluting the organic solvent extract of Fucus obtained by extraction with acetone under the above-mentioned macroporous resin elution conditions of the present invention and the Gentiana macrophylla extract has a further significantly improved sunscreen effect compared to the combination of the Fucus extract obtained by extraction with acetone and the Gentiana macrophylla extract; it has an excellent sunscreen effect.

[0042] As can be seen from the experimental results in Table 1, the cell survival rates of experimental groups 5 and 6 are higher than that of experimental group 1, but the increase is not significant; the increase is far less than that of experimental group 2. This indicates that: the macroporous resin elution conditions of the present invention are very crucial; only when the combination of the Fucus extract obtained by further eluting the organic solvent extract of Fucus obtained by extraction with acetone under the above-mentioned macroporous resin elution conditions of the present invention and the Gentiana macrophylla extract can the sunscreen effect be further significantly improved; however, when the combination of the Fucus extract obtained by further eluting the organic solvent extract of Fucus obtained by extraction with acetone under other macroporous resin elution conditions and the Gentiana macrophylla extract, the sunscreen effect cannot be further significantly improved.

Claims

1. A composition containing fucus vesiculosus extract, characterized in that, It contains fucus extract and gentiana macrophylla extract; Among them, the weight ratio of the fucus extract to the gentiana macrophylla extract is 1 to 10:1 to 10.

2. The composition containing extract of C. fusiforme according to claim 1, characterized in that, The weight ratio of the fucus extract to the gentiana macrophylla extract is 1 to 5:1 to 5; Further preferably, the weight ratio of the fucus extract to the gentiana macrophylla extract is 1 to 3:1 to 3; Most preferably, the weight ratio of the fucus extract to the gentiana macrophylla extract is 1:

1.

3. The composition containing extract of C. fusiforme according to claim 1, characterized in that, The fucus extract is prepared by the following method: Take fucus, extract it with an organic solvent. After the extraction is completed, take the extract, and concentrate and dry the extract to obtain the fucus organic solvent extract; take the fucus organic solvent extract to obtain the fucus extract.

4. The composition containing extract of C. fusiformis according to claim 3, characterized in that, The material-liquid ratio of fucus to the organic solvent is 1 kg:5 to 15 L.

5. The composition containing extract of C. fusiforme according to claim 3, characterized in that, The organic solvent is acetone.

6. The composition containing extract of C. fusiforme according to claim 1, wherein, The gentiana macrophylla extract is prepared by the following method: Take gentiana macrophylla, extract it with an organic solvent. After the extraction is completed, take the extract, and concentrate and dry the extract to obtain the gentiana macrophylla organic solvent extract; take the gentiana macrophylla organic solvent extract to obtain the gentiana macrophylla extract.

7. The composition containing extract of C. fusiforme according to claim 6, characterized in that, The material-liquid ratio of gentiana macrophylla to the organic solvent is 1 kg:5 to 15 L.

8. The composition containing the extract of C. fusiformis according to claim 7, wherein The organic solvent is ethanol.

9. Use of the composition containing fucus extract according to any one of claims 1 to 8 in the preparation of a product having a sunscreen effect.

10. The application according to claim 9, wherein The product is a sunscreen.

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