Anti-hair loss and hair growth essence and preparation method thereof

Abstract

The invention provides anti-hair loss and hair growth essence and a preparation method thereof, and relates to the technical field of cosmetics. According to the anti-hair loss and hair growth essence disclosed by the invention, various natural plant extracts, amino acids, peptides and trace elements are combined and proportioned, so that the anti-hair loss and hair growth essence has the synergistic interaction functions of preventing hair loss, promoting hair growth, diminishing inflammation, inhibiting bacteria, resisting oxidation, activating blood, supplying nutrients and the like. Through introduction of green technologies such as supercritical fluid extraction-supercritical fluid chromatographic separation and the like, use and residues of organic solvents can be effectively reduced, and the enrichment degree of functional components is remarkably improved. Compared with other anti-hair-loss essence taking natural plant extracts as functional components, the essence disclosed by the invention has the advantages that the additive amount of the extracts in the essence is less, and side effects are avoided. The product has the advantages of mildness, safety, no irritation, no cleaning and the like.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and particularly to an anti - hair - loss and hair - growth promoting essence and a preparation method thereof. Background Art

[0002] In recent years, scalp health problems such as hair loss, sparse hair, and receding hairline have increasingly attracted people's attention. Androgenetic alopecia is a common hair - loss disease, usually accompanied by problems such as excessive sebum secretion on the scalp. When the androgen level in the body is imbalanced, testosterone is catalyzed by 5α - reductase in the body to generate dihydrotestosterone. Both testosterone and dihydrotestosterone can bind to androgen receptors, increase the expression of transforming growth factor TGF - β1, TGF - β2, and type I procollagen, prolong the resting phase of hair follicles, induce apoptosis of dermal papilla cells, promote the development of perifollicular fibrosis, and ultimately lead to hair follicle miniaturization, thus causing androgenetic alopecia. Folliculitis caused by bacteria and fungal infections such as Staphylococcus aureus, Pseudomonas aeruginosa, Malassezia, and apoptosis of hair follicle cells induced by lipid peroxidation are also important causes of hair loss.

[0003] Currently, some enzyme inhibitors are usually added to anti - hair - loss and hair - growth promoting products on the market, such as finasteride and dutasteride. By inhibiting the activity of 5α - reductase, the production amount of dihydrotestosterone is reduced, thereby reducing hair loss. Or adding minoxidil to shorten the resting phase of hair growth, increase the proportion of hair follicles in the growth phase, and increase the number of hairs. Adding antibacterial agents such as zinc pyrithione is also a common solution. Although the above - mentioned methods have significant effects, they all have certain side effects. Finasteride and dutasteride may cause sexual dysfunction, and minoxidil may cause hirsutism or allergic dermatitis. Zinc pyrithione has certain toxicity and has been listed as a prohibited ingredient in cosmetics by the European Union. Selecting natural plant extracts (mainly traditional Chinese medicinal materials) with high safety has become a hot topic. Extracts of Polygonum multiflorum Thunb. and Sesamum indicum L. are commonly used active ingredients for blackening hair. Extracts of Platycladus orientalis (L.) Franco, Panax ginseng C. A. Mey., and Prunus armeniaca L. are choices for hair - growth promoting and anti - hair - loss formulas. Extracts of Lonicera japonica Thunb. and Chrysanthemum indicum L. are used because of their antibacterial and anti - inflammatory characteristics. Extracts of Camellia nitidissima Chi are antioxidant active ingredients. Extracts of Zingiber officinale Roscoe are used for their effects of promoting blood circulation and providing nutrition. However, at present, most anti - hair - loss essences featuring plant extracts only play therapeutic effects in some fields such as blackening hair, hair growth, anti - hair - loss, anti - bacterial and anti - inflammatory, promoting blood circulation to supply nutrients, and antioxidant, rather than starting from the whole and achieving multiple - pronged and synergistic effects. At the same time, the extraction process of plant extracts mostly uses traditional water or alcohol - based reagents, resulting in problems such as low content of active ingredients, many impurities, and solvent residues. To achieve anti - hair - loss and hair - growth promoting effects, the addition amount is often relatively large, and the presence of impurities or solvents will greatly increase potential risks such as sensitization.

[0004] In view of this, the present invention is specifically proposed. Summary of the Invention

[0005] The first object of the present invention is to provide an anti - hair - loss and hair - growth essence to solve the above - mentioned technical problems.

[0006] The second object of the present invention is to provide a preparation method of the above - mentioned anti - hair - loss and hair - growth essence.

[0007] In order to achieve the above objects, the following technical solutions are specifically adopted:

[0008] In the first aspect, the present invention provides an anti - hair - loss and hair - growth essence, which, by mass percentage, includes a solvent, a humectant, an antioxidant, a chelating agent, a surfactant, other trace components, and the balance is water;

[0009] The solvent is a composition of butylene glycol, methyl propylene glycol, 1,2 - hexanediol, and propylene glycol;

[0010] The other trace components include 0.1% - 0.3% Platycladus orientalis leaf extract, 0.002% - 0.006% acetyl tetrapeptide - 3, 0.0005% - 0.002% Larix decidua extract, 0.0005% - 0.0025% Sophora japonica flower bud extract, 0.01% - 0.04% tea extract, 0.02% - 0.06% Rehmannia glutinosa root extract, 0.015% - 0.05% Swertia japonica extract, 0.0004% - 0.001% Glycine soja seed extract, 0.002% - 0.012% Larix sibirica extract, 0.0005% - 0.0025% glycine, and 0.00005% - 0.0002% zinc chloride.

[0011] As a further technical solution, the mass ratio of butylene glycol, methyl propylene glycol, 1,2 - hexanediol, and propylene glycol is 920:70:1:9.

[0012] As a further technical solution, the other trace components include 0.2% - 0.25% Platycladus orientalis leaf extract, 0.004% - 0.005% acetyl tetrapeptide - 3, 0.00075% - 0.00125% Larix decidua extract, 0.001% - 0.002% Sophora japonica flower bud extract, 0.01% - 0.02% tea extract, 0.04% - 0.05% Rehmannia glutinosa root extract, 0.02% - 0.04% Swertia japonica extract, 0.0006% - 0.0009% Glycine soja seed extract, 0.006% - 0.01% Larix sibirica extract, 0.00075% - 0.00125% glycine, and 0.000075% - 0.000125% zinc chloride.

[0013] As a further technical solution, the humectant includes one or more of glycerol and sodium hyaluronate.

[0014] As a further technical solution, the antioxidant includes one or more of p-hydroxyacetophenone and sodium metabisulfite.

[0015] As a further technical solution, the chelating agent includes one or more of octanoyl hydroxamic acid and disodium EDTA.

[0016] As a further technical solution, the surfactant includes one or more of glyceryl caprylate and PEG-40 hydrogenated castor oil.

[0017] As a further technical solution, the extracts of Platycladus orientalis leaves, European larch wood, Sophora japonica flower buds, Rehmannia glutinosa roots, and Siberian larch wood are extracted by a supercritical fluid extraction-supercritical fluid chromatography process;

[0018] The conditions for the supercritical fluid extraction are as follows:

[0019] Extraction pressure: 20 - 30 MPa, extraction temperature: 45 - 50 °C, static extraction time: 1 - 2 h, dynamic extraction time: 0.5 - 1 h, separation pressure: 10 - 16 MPa, CO2 flow rate: 20 - 25 L / h, entrainer: one or more of water, methanol, and ethanol;

[0020] The conditions for the supercritical fluid chromatography are as follows:

[0021] Separation material: Diol (diol-based), modifier: one or more of water, methanol, and ethanol, column temperature: 35 - 40 °C, back pressure: 15 - 20 MPa, flow rate: 200 - 280 mL / min; modifier change: 0 - 15 min, 5 - 30%; collect the fraction from 0 - 5 min.

[0022] In a second aspect, the present invention provides a method for preparing the above anti-hair loss and hair growth essence, including the following steps:

[0023] First, mix the solvent, the extract of Platycladus orientalis leaves, the extract of Rehmannia glutinosa roots, and water, and then add the humectant, antioxidant, chelating agent, surfactant, acetyl tetrapeptide-3, the extract of European larch wood, the extract of Sophora japonica flower buds, the extract of tea leaves, the extract of Swertia japonica, the extract of wild soybean seeds, the extract of Siberian larch wood, glycine, and zinc chloride for a second mixing to prepare the anti-hair loss and hair growth essence.

[0024] As a further technical solution, the temperature for the first mixing is 75 - 85 °C;

[0025] The temperature for the second mixing is 45 - 50 °C.

[0026] Compared with the prior art, the present invention has the following beneficial effects:

[0027] The anti - hair - loss and hair - growth promoting essence disclosed by the present invention, through the combined ratio of various natural plant extracts, amino acids, peptides and trace elements, plays a synergistic effect of anti - hair - loss, hair - growth promotion, anti - inflammation and bacteriostasis, antioxidant, promoting blood circulation and supplying nutrients. By introducing green technologies such as supercritical fluid extraction (SFE) - supercritical fluid chromatography (SFC) separation, it can effectively reduce the use and residue of organic solvents and significantly improve the enrichment degree of active ingredients. Compared with other anti - hair - loss essences with natural plant extracts as active ingredients, the addition amount of extracts in the essence involved in the present invention is less, avoiding the generation of side effects. This product has the advantages of mildness, safety, no irritation, and no need for washing. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following will briefly introduce the drawings required for the description of the specific embodiments or the prior art. Obviously, the following drawings are some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0029] Figure 1 It is a comparison diagram before and after using the anti - hair - loss and hair - growth promoting essence in Example 1. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0030] The following will describe the embodiments of the present invention in detail in combination with the embodiments and examples. However, those skilled in the art will understand that the following embodiments and examples are only used to illustrate the present invention and should not be regarded as limiting the scope of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention. Those not specifying specific conditions are carried out according to conventional conditions or conditions recommended by the manufacturer. Those reagents or instruments not specifying the manufacturer can be obtained as conventional products through commercial purchase.

[0031] In the first aspect, the present invention provides an anti - hair - loss and hair - growth promoting essence, which, by mass percentage, includes a solvent, a humectant, an antioxidant, a chelating agent, a surfactant, other trace components, and the balance is water;

[0032] The solvent is a composition of butylene glycol, methyl propylene glycol, 1,2 - hexanediol and propylene glycol;

[0033] The other trace components include 0.1% - 0.3% Platycladus orientalis leaf extract, 0.002% - 0.006% acetyl tetrapeptide-3, 0.0005% - 0.002% European larch wood extract, 0.0005% - 0.0025% Sophora japonica flower bud extract, 0.01% - 0.04% tea extract, 0.02% - 0.06% Rehmannia glutinosa root extract, 0.015% - 0.05% Swertia japonica extract, 0.0004% - 0.001% Glycine soja seed extract, 0.002% - 0.012% Siberian larch wood extract, 0.0005% - 0.0025% glycine and 0.00005% - 0.0002% zinc chloride.

[0034] The Platycladus orientalis leaf extract is rich in flavonoids, which can promote hair growth, inhibit hair loss, reduce inflammation and antibacterial, reduce sebum secretion, and improve the scalp microenvironment. Acetyl tetrapeptide-3 can stimulate the synthesis of extracellular matrix proteins of dermal papilla cells, increase the volume of hair follicles, and promote the anchoring of hair in hair follicles; European larch wood extract and Siberian larch wood extract are rich in antioxidant and anti-allergic components, which can reduce oxidative stress and improve hair follicle health; the main active ingredient in Sophora japonica flower bud extract is luteolin, which has antibacterial and anti-inflammatory effects and can improve the scalp environment; tea extract can scavenge reactive oxygen free radicals, prevent scalp aging, and strengthen hair strands; Rehmannia glutinosa root extract can accelerate blood circulation, enhance scalp metabolism, and has anti-aging and anti-inflammatory effects; Swertia japonica extract can promote blood circulation and autophagy activity of dermal papilla cells, and activate hair follicle cells; Glycine soja seed extract can provide the nutrients required for hair, and play a role in scalp moisturizing and antioxidant; glycine is beneficial to the synthesis of hair keratin and promotes hair growth; zinc chloride can supplement the zinc element in hair and help stabilize the molecular structure of hair proteins. Through the synergistic cooperation of the above components, the essence has a good effect of preventing hair loss and promoting hair growth. Among them, Platycladus orientalis leaf extract, European larch wood extract, Sophora japonica flower bud extract, Rehmannia glutinosa root extract, Siberian larch wood extract and acetyl tetrapeptide-3 are the key efficacy components of this anti-hair loss and hair growth essence. Among the key efficacy components, the components with anti-hair loss and hair growth effects are: Platycladus orientalis leaf extract, acetyl tetrapeptide-3; blood-activating and nourishing components: Rehmannia glutinosa root extract; anti-inflammatory and antibacterial components: Platycladus orientalis leaf extract, Sophora japonica flower bud extract; antioxidant components: European larch wood extract and Siberian larch wood extract; sebum balance components: Platycladus orientalis leaf extract and Rehmannia glutinosa root extract. Through the research of the inventor, it is found that the combined ratio of the key efficacy components plays a crucial role in the effects of the essence such as preventing hair loss and promoting hair growth, anti-inflammatory and antibacterial, antioxidant and improving the scalp microenvironment.

[0035] In the present invention, there are no specific restrictions on the dosages of the solvent, humectant, antioxidant, chelating agent, and surfactant. For example, in the anti - hair - loss and hair - growth promoting essence, the mass ratio of the solvent can be 12%, the mass ratio of the humectant can be 0.2%, the mass ratio of the antioxidant can be 0.4%, the mass ratio of the chelating agent can be 0.075%, and the mass ratio of the surfactant can be 0.04%.

[0036] In some alternative embodiments, the mass ratio of butanediol, methylpropanediol, 1,2 - hexanediol, and propylene glycol is 920:70:1:9.

[0037] In some alternative embodiments, by mass percentage, the other trace components include 0.2% - 0.25% Sabina chinensis leaf extract, 0.004% - 0.005% acetyl tetrapeptide - 3, 0.00075% - 0.00125% Larix decidua extract, 0.001% - 0.002% Sophora japonica flower bud extract, 0.01% - 0.02% tea extract, 0.04% - 0.05% Rehmannia glutinosa root extract, 0.02% - 0.04% Swertia japonica extract, 0.0006% - 0.0009% Glycine soja seed extract, 0.006% - 0.01% Larix sibirica extract, 0.00075% - 0.00125% glycine, and 0.000075% - 0.000125% zinc chloride.

[0038] By further optimizing and adjusting each component in the anti - hair - loss and hair - growth promoting essence, the anti - hair - loss and hair - growth promoting effect of the essence is better.

[0039] In some alternative embodiments, the humectant includes one or more of glycerol and sodium hyaluronate;

[0040] Preferably, the humectant consists of glycerol and sodium hyaluronate, and the mass ratio of glycerol to sodium hyaluronate is (4 - 6):1.

[0041] Selecting the above - mentioned solvent and humectant is beneficial to the dissolution of the active ingredients and their absorption on the scalp.

[0042] In some alternative embodiments, the antioxidant includes one or more of p - hydroxyacetophenone and sodium metabisulfite;

[0043] Preferably, the antioxidant consists of p - hydroxyacetophenone and sodium metabisulfite, and the mass ratio of p - hydroxyacetophenone to sodium metabisulfite is (300 - 350):1.

[0044] In some alternative embodiments, the chelating agent includes one or more of octanoyl hydroxamic acid and disodium EDTA;

[0045] Preferably, the chelating agent consists of octanohydroxamic acid and disodium EDTA, and the mass ratio of octanohydroxamic acid to disodium EDTA is (0.8 - 1):1.

[0046] Selecting the above antioxidants and chelating agents can keep the active ingredients stable and play a long - term role.

[0047] In some alternative embodiments, the surfactant includes one or more of glyceryl caprylate and PEG - 40 hydrogenated castor oil;

[0048] Preferably, the surfactant consists of glyceryl caprylate and PEG - 40 hydrogenated castor oil, and the mass ratio of glyceryl caprylate to PEG - 40 hydrogenated castor oil is 1:(75 - 80).

[0049] In some alternative embodiments, it further includes essence.

[0050] In some alternative embodiments, the extracts of Platycladus orientalis leaves, European larch wood, Sophora japonica flower buds, Rehmannia glutinosa roots and Larix sibirica wood are extracted by supercritical fluid extraction - supercritical fluid chromatography process;

[0051] The conditions for the supercritical fluid extraction are as follows:

[0052] Extraction pressure: 20 - 30 MPa, extraction temperature: 45 - 50 °C, static extraction time: 1 - 2 h, dynamic extraction time: 0.5 - 1 h, separation pressure: 10 - 16 MPa, CO2 flow rate: 20 - 25 L / h, entrainer: one or more of water, methanol and ethanol;

[0053] The conditions for the supercritical fluid chromatography are as follows:

[0054] Separation material: Diol, modifier: one or more of water, methanol and ethanol, column temperature: 35 - 40 °C, back pressure: 15 - 20 MPa, flow rate: 200 - 280 mL / min; modifier change: 0 - 15 min, 5 - 30%; collect the fractions from 0 - 5 min.

[0055] In some alternative embodiments, the tea extract and the wild soybean seed extract are water extracts, and the Swertia japonica extract is an ethanol extract. For example, the tea extract, Swertia japonica extract and wild soybean seed extract from Jiangsu Caiwei Biotechnology Co., Ltd. can be selected.

[0056] In the second aspect, the present invention provides a preparation method of the above anti - hair - loss and hair - growth essence, including the following steps:

[0057] First, mix the solvent, Platycladus orientalis leaf extract, Rehmannia glutinosa root extract, and water. Then, add a humectant, an antioxidant, a chelating agent, a surfactant, acetyl tetrapeptide-3, Larix decidua Mill. extract, Sophora japonica L. flower bud extract, Camellia sinensis leaf extract, Swertia japonica (Thunb.) Makino extract, Glycine max (L.) Merr. seed extract, Larix sibirica Ledeb. extract, glycine, and zinc chloride for a second mixing to prepare an anti-hair loss and hair growth essence.

[0058] The preparation method is simple and convenient. The prepared anti-hair loss and hair growth essence has good stability and is suitable for daily care and long-term use.

[0059] In some alternative embodiments, the temperature of the first mixing can be, for example, but not limited to, 75°C, 80°C, or 85°C;

[0060] The temperature of the second mixing can be, for example, but not limited to, 45°C, 48°C, or 50°C.

[0061] The present invention will be further illustrated by specific examples and comparative examples below. However, it should be understood that these examples are only for more detailed illustration and should not be construed as limiting the present invention in any way.

[0062] In the following examples and comparative examples, the Camellia sinensis leaf extract (aqueous extract), Swertia japonica (Thunb.) Makino extract (ethanol extract), and Glycine max (L.) Merr. seed extract (aqueous extract) were purchased from Jiangsu Caiwei Biotechnology Co., Ltd.

[0063] Example 1

[0064] An anti-hair loss and hair growth essence, by mass percentage, includes 12% solvent, 0.2% humectant, 0.4% antioxidant, 0.075% chelating agent, 0.04% surfactant, other trace components, and the balance is water;

[0065] The solvent is composed of butylene glycol, methyl propylene glycol, 1,2 - hexanediol, and propylene glycol; the mass ratio of butylene glycol, methyl propylene glycol, 1,2 - hexanediol, and propylene glycol is 920:70:1:9;

[0066] The humectant is composed of glycerin and sodium hyaluronate, and the mass ratio of glycerin and sodium hyaluronate is 5:1;

[0067] The antioxidant is composed of p - hydroxyacetophenone and sodium metabisulfite, and the mass ratio of p - hydroxyacetophenone and sodium metabisulfite is 320:1;

[0068] The chelating agent is composed of octanoyl hydroxamic acid and disodium EDTA, and the mass ratio of octanoyl hydroxamic acid and disodium EDTA is 0.9:1;

[0069] The surfactant consists of glyceryl caprylate and PEG-40 hydrogenated castor oil, and the mass ratio of glyceryl caprylate to PEG-40 hydrogenated castor oil is 1:78;

[0070] Other trace components include 0.23% Platycladus orientalis leaf extract, 0.0045% acetyl tetrapeptide-3, 0.001% European larch wood extract, 0.0015% Sophora japonica flower bud extract, 0.018% tea extract, 0.045% Rehmannia glutinosa root extract, 0.03% Swertia japonica extract, 0.0007% Glycine soja seed extract, 0.008% Siberian larch wood extract, 0.001% glycine and 0.0001% zinc chloride.

[0071] Among them, the preparation methods of Platycladus orientalis leaf extract, European larch wood extract, Sophora japonica flower bud extract, Rehmannia glutinosa root extract and Siberian larch wood extract are as follows:

[0072] (1) Platycladus orientalis leaves, European larch wood, Sophora japonica flower buds, Rehmannia glutinosa roots and Siberian larch wood are washed, dried and crushed.

[0073] (2) The powders of Platycladus orientalis leaves, European larch wood, Sophora japonica flower buds, Rehmannia glutinosa roots and Siberian larch wood are used with supercritical fluid extraction (SFE)-supercritical fluid chromatography (SFC) as the extraction process. The specific operations are as follows: The above powders are respectively placed in a supercritical fluid extraction tank. The extraction pressure: 20-30 MPa, the extraction temperature: 45-50 °C, the static extraction time: 1-2 h, the dynamic extraction time: 0.5-1 h, the separation pressure: 10-16 MPa, the CO2 flow rate: 20-25 L / h, the entrainer: methanol. The extracted components are separated by supercritical fluid chromatography: The separation material: Diol, the modifier: methanol, the column temperature: 35-40 °C, the back pressure: 15-20 MPa, the flow rate: 200-280 mL / min; the change of the modifier: 0-15 min, 5-30%. Collect the fractions from 0 to 5 min to obtain Platycladus orientalis leaf extract, European larch wood extract, Sophora japonica flower bud extract and Siberian larch wood extract respectively. The active components of Platycladus orientalis leaves extracted by this method: 45-50% flavonoids, 30-35% terpenoids and volatile oils, and the contents of anti-hair loss and hair growth promoting components such as quercetin, apigenin, kaempferol and pinoresinol are all above 2%; The active components of European larch wood: 55% flavonoids, 5% dihydroquercetin; The active components of Sophora japonica flower buds: 30-40% essential oils, 40-45% flavonoids, and the contents of rutin, quercetin and kaempferol are above 2%; The active components of Rehmannia glutinosa roots: 40-50% polysaccharides; The active components of Siberian larch wood: 15-20% volatile oils, 15% ketones, 15-20% terpenes and 5-10% aldehydes. The above extracts are all off-white powders, light yellow powders or light yellow oily substances.

[0074] The preparation method of the anti - hair - loss and hair - growth essence is as follows:

[0075] (1) According to the above mass percentages, mix the solvent, Platycladus orientalis extract, Rehmannia glutinosa root extract, and water, heat to 75 - 85 °C, stir and disperse evenly at 100 - 200 rpm, and keep warm for 30 min.

[0076] (2) Cool down to 45 - 50 °C, and according to the above mass percentages, add the humectant, antioxidant, chelating agent, surfactant, acetyl tetrapeptide - 3, Larix decidua extract, Sophora japonica flower bud extract, tea extract, Swertia japonica extract, Glycine max seed extract, Larix sibirica extract, glycine, zinc chloride, and stir at 100 - 200 rpm until completely dissolved.

[0077] (3) Cool down to below 40 °C to obtain the anti - hair - loss and hair - growth essence.

[0078] Example 2

[0079] An anti - hair - loss and hair - growth essence, by mass percentage, includes 12% solvent, 0.2% humectant, 0.4% antioxidant, 0.075% chelating agent, 0.04% surfactant, other trace components, and the balance is water;

[0080] The solvent is composed of butylene glycol, methyl propylene glycol, 1,2 - hexanediol, and propylene glycol; the mass ratio of butylene glycol, methyl propylene glycol, 1,2 - hexanediol, and propylene glycol is 920:70:1:9;

[0081] The humectant is composed of glycerol and sodium hyaluronate, and the mass ratio of glycerol and sodium hyaluronate is 4:1;

[0082] The antioxidant is composed of p - hydroxyacetophenone and sodium metabisulfite, and the mass ratio of p - hydroxyacetophenone and sodium metabisulfite is 300:1;

[0083] The chelating agent is composed of octanoyl hydroxamic acid and disodium EDTA, and the mass ratio of octanoyl hydroxamic acid and disodium EDTA is 0.8:1;

[0084] The surfactant is composed of glyceryl caprylate and PEG - 40 hydrogenated castor oil, and the mass ratio of glyceryl caprylate and PEG - 40 hydrogenated castor oil is 1:75;

[0085] Other trace components include 0.1% Platycladus orientalis leaf extract, 0.002% Acetyl Tetrapeptide-3, 0.0005% Larix decidua Mill. extract, 0.0005% Sophora japonica L. flower bud extract, 0.01% tea extract, 0.02% Rehmannia glutinosa Libosch. root extract, 0.015% Swertia japonica (Thunb.) Makino extract, 0.0004% Glycine soja Sieb. et Zucc. seed extract, 0.002% Larix sibirica Ledeb. extract, 0.0005% glycine and 0.00005% zinc chloride.

[0086] The preparation method of other trace components and the preparation method of the anti-hair loss and hair growth essence are the same as those in Example 1.

[0087] Example 3

[0088] An anti-hair loss and hair growth essence, by mass percentage, includes 12% solvent, 0.2% humectant, 0.4% antioxidant, 0.075% chelating agent, 0.04% surfactant, other trace components, and the balance is water;

[0089] The solvent is composed of butanediol, methylpropanediol, 1,2-hexanediol and propylene glycol; the mass ratio of butanediol, methylpropanediol, 1,2-hexanediol and propylene glycol is 920:70:1:9;

[0090] The humectant is composed of glycerol and sodium hyaluronate, and the mass ratio of glycerol and sodium hyaluronate is 6:1;

[0091] The antioxidant is composed of p-hydroxyacetophenone and sodium metabisulfite, and the mass ratio of p-hydroxyacetophenone and sodium metabisulfite is 350:1;

[0092] The chelating agent is composed of octanoyl hydroxamic acid and disodium EDTA, and the mass ratio of octanoyl hydroxamic acid and disodium EDTA is 1:1;

[0093] The surfactant is composed of glyceryl caprylate and PEG-40 hydrogenated castor oil, and the mass ratio of glyceryl caprylate and PEG-40 hydrogenated castor oil is 1:80;

[0094] Other trace components include 0.30% Platycladus orientalis leaf extract, 0.006% Acetyl Tetrapeptide-3, 0.002% Larix decidua Mill. extract, 0.0025% Sophora japonica L. flower bud extract, 0.04% tea extract, 0.06% Rehmannia glutinosa Libosch. root extract, 0.05% Swertia japonica (Thunb.) Makino extract, 0.001% Glycine soja Sieb. et Zucc. seed extract, 0.012% Larix sibirica Ledeb. extract, 0.0025% glycine and 0.0002% zinc chloride.

[0095] The preparation method of other trace components and the preparation method of the anti-hair loss and hair growth essence are the same as those in Example 1.

[0096] Comparative Example 1

[0097] The difference from Example 1 is that no Platycladus orientalis leaf extract is added, and the remaining key active ingredients are scaled up proportionally while keeping the total content of the key active ingredients (i.e., other trace ingredients) unchanged.

[0098] Comparative Example 2

[0099] The difference from Example 1 is that no European larch wood extract is added, and the remaining key active ingredients are scaled up proportionally while keeping the total content of the key active ingredients (i.e., other trace ingredients) unchanged.

[0100] Comparative Example 3

[0101] The difference from Example 1 is that no Sophora japonica flower bud extract is added, and the remaining key active ingredients are scaled up proportionally while keeping the total content of the key active ingredients (i.e., other trace ingredients) unchanged.

[0102] Comparative Example 4

[0103] The difference from Example 1 is that no Rehmannia glutinosa root extract is added, and the remaining key active ingredients are scaled up proportionally while keeping the total content of the key active ingredients (i.e., other trace ingredients) unchanged.

[0104] Comparative Example 5

[0105] The difference from Example 1 is that no Siberian larch wood extract is added, and the remaining key active ingredients are scaled up proportionally while keeping the total content of the key active ingredients (i.e., other trace ingredients) unchanged.

[0106] Comparative Example 6

[0107] The difference from Example 1 is that no Acetyl Tetrapeptide-3 is added, and the remaining key active ingredients are scaled up proportionally while keeping the total content of the key active ingredients (i.e., other trace ingredients) unchanged.

[0108] Comparative Example 7

[0109] The difference from Example 1 is that the preparation methods of Platycladus orientalis leaf extract, European larch wood extract, Sophora japonica flower bud extract, Rehmannia glutinosa root extract and Siberian larch wood extract are different, and the preparation methods are as follows:

[0110] (1) Platycladus orientalis leaves, European larch wood, Sophora japonica flower buds, Rehmannia glutinosa roots and Siberian larch wood are washed, dried and crushed.

[0111] (2) The powder of Platycladus orientalis leaves, European larch wood, Sophora japonica flower buds, Rehmannia glutinosa roots, and Siberian larch wood is extracted by the traditional Soxhlet extraction method. The specific operation is as follows: Add the powder to ethanol at a mass-volume (g / mL) ratio of 1:10 - 15, heat under reflux for 30 - 60 min, filter, concentrate to obtain a filtrate, remove alcohols by vacuum concentration, pass through AB-8 adsorption resin, wash with an alcohol-water solution, concentrate, and freeze-dry. The active ingredients of Platycladus orientalis leaves extracted by this method are: 10 - 15% flavonoids, 5 - 10% terpenoids and volatile oils, and the content of anti-hair loss and hair growth promoting ingredients such as quercetin, apigenin, kaempferol, and pinoresinol is 0.05 - 0.3%; the active ingredients of European larch wood are: 20% flavonoids, 0.6% dihydroquercetin; the active ingredients of Sophora japonica flower buds are: 3 - 5% essential oils, 10 - 15% flavonoids, and the content of rutin, quercetin, and kaempferol is 0.1 - 0.45%; the active ingredients of Rehmannia glutinosa roots are: 10 - 15% polysaccharides; the active ingredients of Siberian larch wood are: 3 - 5% volatile oils, 1 - 3% ketones, 3 - 5% terpenes, and 1 - 2% aldehydes. The above extracts are all brown powders or brown viscous substances.

[0112] Comparative Example 8

[0113] The difference from Comparative Example 7 is that the addition amounts of the extracts of Platycladus orientalis leaves, European larch wood, Sophora japonica flower buds, Rehmannia glutinosa roots, and Siberian larch wood are increased by 5 times.

[0114] Performance Test

[0115] Test Example 1

[0116] Human patch skin irritation test: Recruit 60 volunteers aged 20 - 59 years old, with a male-female ratio of 1:1. The volunteers have not had hair beauty treatments such as hair dyeing and perming in the past 1 month. Among them, patients with severe androgenetic alopecia, alopecia areata, inflammatory cicatricial alopecia, or other scalp / hair diseases, and those with completely necrotic hair follicles are not included in the test volunteer scope of the present invention. The specific operation is as follows: Drop the products of the examples and comparative examples on the filter paper of the patch tester respectively, attach the patch tester to the forearm of the subject with non-irritating tape, press to evenly apply the product on the skin, and keep it for 48 h; remove the patch tester, wait for the indentation to disappear, observe the skin reaction, and count the number of people in each grade. Table 1 is the identification standard. The results are shown in Table 2. The results show that no adverse reactions such as erythema and papules occurred in Example 1 and Comparative Examples 1 - 6. Four people had mild erythema when using the essence in Comparative Example 7. In Comparative Example 8, 10 participants had mild erythema and 2 had erythema as adverse reactions. It can be shown from this that the extract obtained by the SFE-SFC process added to the essence is more mild and non-irritating. This may be related to fewer impurities in the extract of the SFE-SFC process.

[0117] Table 1 Identification Criteria for Skin Adverse Reactions

[0118] Level Identification standard 0 No irritation, no erythema 1 Mild erythema 2 Erythema 3 Erythema, papules, blisters 4 Severe edema, large blisters 。

[0119] Table 2 Results of Human Patch Skin Irritation Test

[0120]

[0121] Test Example 2

[0122] Antibacterial Test: Refer to the antibacterial experiment of the anti - hair loss essence against Malassezia in "An Anti - hair Loss Essence and Its Preparation Method" (Patent Application No.: 202411161890.4). The specific operation is as follows: The freeze - dried powder of Malassezia is added to physiological saline and suspended, and then spread on a solid medium with a sterile cotton swab for culture. The strains of 2 - 5 generations are selected for the test. The strains are prepared into a suspension of 0.5×10 8 CFU / mL with physiological saline. An appropriate amount of the suspension is dipped with a cotton swab and evenly spread on the solid medium. The essence of Example 1 and Comparative Examples 1 - 8 are taken respectively, and dissolved and diluted to 1.0 mg / mL with 50wt% DMSO - physiological saline solution. A sterile Oxford cup is placed on the surface of the solid medium, and 0.1 mL of each test sample is added to the cup and cultured for 48 h. The diameter of the antibacterial circle is measured with a vernier caliper. Each group of experiments is measured in parallel 3 times, and the average value is taken. Table 3 shows the antibacterial test data. It can be seen from the antibacterial test results that Examples 1 - 3, Comparative Example 2, and Comparative Examples 4 - 6 all show good antibacterial effects. The antibacterial circles of Comparative Example 1 and Comparative Example 3 are significantly reduced, which is related to the lack of Platycladus orientalis leaf extract and Sophora japonica flower bud extract with anti - inflammatory and antibacterial effects in the essence. The antibacterial effect of Comparative Example 7 is inferior to that of Example 1, perhaps because the active ingredients in the Platycladus orientalis leaf extract and Sophora japonica flower bud extract obtained by the Soxhlet extraction method are less. Comparative Example 8 shows the best antibacterial activity, perhaps due to a significant increase in the content of the key efficacy extracts.

[0123] Table 3 Antibacterial Test

[0124] Test group Diameter of inhibition zone Test group Diameter of inhibition zone Example 1 21mm Comparative example 5 20mm Comparative example 1 13mm Comparative example 6 20mm Comparative example 2 20mm Comparative example 7 17mm Comparative example 3 15mm Comparative example 8 25mm Comparative example 4 20mm Example 2 19mm Example 3 23mm 。

[0125] Test Example 3

[0126] Anti - hair loss effect test and hair density test: Before this test, testers who had adverse reactions in the "Human Patch Skin Irritation Test" were excluded. A total of 90 volunteers aged 20 - 60 years old were recruited and evenly divided into 9 groups with a male - female ratio of 1:1. The subjects applied 3 mL of the essence evenly to the scalp every night, massaged for 1 - 2 minutes until completely absorbed, and did this continuously for 12 weeks. Each volunteer used the same non - hair - growing shampoo 2 - 3 times a week during these 12 weeks of use. Comb the hair every 4 weeks, collect the fallen hair and count. At the same time, use a micro - camera to shoot the hair growth situation, evaluate the hair density, and finally take the average value. Hair loss reduction rate = (number of hairs lost on the first day - number of hairs lost on the 12th week) / number of hairs lost on the first day × 100%. Hair density increase rate = (hair density on the 12th week - hair density on the first day) / hair density on the first day × 100%. The data are listed in Tables 4 and 5. Figure 1 It is a comparison chart before and after using Example 1.

[0127] Table 4 Average number of fallen hairs (roots)

[0128]

[0129] Table 5 Hair density

[0130]

[0131] In Example 1, both the hair loss reduction rate and the hair density increase rate are the highest, showing the best anti - hair loss and hair - growing effects. Figure 1 The comparison chart before and after using the anti - hair loss and hair - growing essence of Example 1 is shown. Both the extract of Platycladus orientalis and acetyl tetrapeptide - 3 have anti - hair loss and hair - growing effects. In Comparative Example 1 and Comparative Example 6 lacking these two active ingredients, both indicators of the hair loss reduction rate and the hair density increase rate are the lowest. In Comparative Examples 2 - 5, one of the active ingredients is missing, and the effects in terms of hair loss and hair growth are both lower than those of Example 1, indicating that the extracts of Larix decidua Mill., Sophora japonica L. bud, Rehmannia glutinosa Libosch. root, and Larix sibirica Ledeb. also play a certain synergistic effect in anti - hair loss and hair growth. Although the addition amount of active ingredients in Comparative Example 7 is the same as that in Example 1, the effect is very different, further indicating that the SFE - SFC extraction process used in the present invention can effectively increase the content of active ingredients and reduce the addition amount of the extract. The effect of Comparative Example 8 is equivalent to that of Example 1, but the addition amount is increased by 5 times, increasing the risk of adverse reactions and the cost of the essence.

[0132] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements on some or all of the technical features; and these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. An anti - hair loss and hair growth essence, characterized in that, By mass percentage, it includes a solvent, a humectant, an antioxidant, a chelating agent, a surfactant, other trace components, and the balance is water; The solvent is a composition of butanediol, methylpropanediol, 1,2 - hexanediol and propylene glycol; The other trace components include 0.1% - 0.3% Platycladus orientalis leaf extract, 0.002% - 0.006% acetyl tetrapeptide - 3, 0.0005% - 0.002% European larch wood extract, 0.0005% - 0.0025% Sophora japonica flower bud extract, 0.01% - 0.04% tea extract, 0.02% - 0.06% Rehmannia glutinosa root extract, 0.015% - 0.05% Swertia japonica extract, 0.0004% - 0.001% Glycine soja seed extract, 0.002% - 0.012% Siberian larch wood extract, 0.0005% - 0.0025% glycine and 0.00005% - 0.0002% zinc chloride.

2. The anti - hair - loss and hair - growth essence according to claim 1, wherein, The mass ratio of butanediol, methylpropanediol, 1,2 - hexanediol and propylene glycol is 920:70:1:

9.

3. The anti-hair loss and hair growth essence according to claim 1, wherein By mass percentage, the other trace components include 0.2% - 0.25% Platycladus orientalis leaf extract, 0.004% - 0.005% acetyl tetrapeptide - 3, 0.00075% - 0.00125% European larch wood extract, 0.001% - 0.002% Sophora japonica flower bud extract, 0.01% - 0.02% tea extract, 0.04% - 0.05% Rehmannia glutinosa root extract, 0.02% - 0.04% Swertia japonica extract, 0.0006% - 0.0009% Glycine soja seed extract, 0.006% - 0.01% Siberian larch wood extract, 0.00075% - 0.00125% glycine and 0.000075% - 0.000125% zinc chloride.

4. The anti-hair loss and hair growth essence according to claim 1, wherein The humectant includes one or more of glycerol and sodium hyaluronate.

5. The anti - hair loss and hair growth essence according to claim 1, characterized in that, The antioxidant includes one or more of p - hydroxyacetophenone and sodium metabisulfite.

6. The anti - hair - loss and hair - growth essence according to claim 1, wherein, The chelating agent includes one or more of octanoyl hydroxamic acid and disodium EDTA.

7. The anti - hair - loss and hair - growth essence according to claim 1, characterized in that, The surfactant includes one or more of glyceryl caprylate and PEG - 40 hydrogenated castor oil.

8. The anti-hair loss and hair growth essence according to claim 1, wherein The Platycladus orientalis leaf extract, European larch wood extract, Sophora japonica flower bud extract, Rehmannia glutinosa root extract and Siberian larch wood extract are extracted by supercritical fluid extraction - supercritical fluid chromatography process; The conditions of the supercritical fluid extraction are as follows: Extraction pressure: 20 - 30 MPa, extraction temperature: 45 - 50 °C, static extraction time: 1 - 2 h, dynamic extraction time: 0.5 - 1 h, separation pressure: 10 - 16 MPa, CO2 flow rate: 20 - 25 L / h, entrainer: one or more of water, methanol and ethanol; The conditions of the supercritical fluid chromatography are as follows: Separation material: Diol, Modifier: one or several of water, methanol and ethanol, Column temperature: 35 - 40 °C, Back pressure: 15 - 20 MPa, Flow rate: 200 - 280 mL / min; Modifier change: 0 - 15 min, 5 - 30%; Collect the fraction of 0 - 5 min.

9. The preparation method of the anti - hair - loss and hair - growth essence according to any one of claims 1 to 8, characterized in that, It includes the following steps: First, mix the solvent, Platycladus orientalis leaf extract, Rehmannia glutinosa root extract and water, and then add a humectant, an antioxidant, a chelating agent, a surfactant, acetyl tetrapeptide-3, Larix decidua Mill. extract, Sophora japonica L. flower bud extract, tea extract, Swertia japonica extract, Glycine soja seed extract, Larix sibirica Ledeb. extract, glycine and zinc chloride for a second mixing to prepare an anti-hair loss and hair growth essence.

10. The preparation method according to claim 9, characterized in that, The temperature of the first mixing is 75 - 85 °C; The temperature of the second mixing is 45 - 50 °C.

Images