Skin whitening compound preparation as well as preparation method and application thereof

Abstract

The invention discloses a skin whitening compound preparation and a preparation method and application thereof, the skin whitening compound preparation is mainly composed of a pouzolzia zeylanica extract and vitamin E, the content of the pouzolzia zeylanica extract is 5 wt%-10 wt%, the content of the vitamin E is 1 wt%-5 wt%, and the balance is pharmaceutically acceptable auxiliary materials. The prepared compound preparation based on pouzolzia zeylanica and vitamin E has a good skin whitening effect. According to the compound preparation, through the synergistic effect of the pouzolzia zeylanica extract and the vitamin E, the activity of tyrosinase can be inhibited, so that generation of melanin is inhibited, the skin color uniformity is improved, the skin moisture content is increased, and the moisturizing function is achieved. The compound preparation is safe and non-irritant, is suitable for long-term use, and can be widely applied to the field of cosmetics or medicines.

Description

Technical Field

[0001] The present invention belongs to the technical field of compound preparations with whitening effects, and specifically relates to a skin whitening compound preparation, its preparation method and application. Background Art

[0002] Many of the current whitening products on the market have problems such as poor effects, easy recurrence or poor safety. Even long-term use can cause serious irritation to the skin. Pouzolzia zeylanica (L.) Benn., as a traditional Chinese medicine, its extract can have antioxidant effects and also has certain anti-inflammatory and lipid metabolism regulating effects. Vitamin E, as an antioxidant, can protect cells from damage by free radicals and plays an important role in maintaining and repairing the skin. However, there is currently no relevant report on combining Pouzolzia zeylanica with vitamin E as a compound preparation for whitening products. Summary of the Invention

[0003] The technical problem solved by the present invention is to provide a skin whitening compound preparation, its preparation method and application. This compound preparation has a significant skin whitening effect and is safe and non-irritating.

[0004] The present invention adopts the following technical solution to solve the above technical problem. A skin whitening compound preparation is characterized in that it mainly consists of Pouzolzia zeylanica extract and vitamin E. Among them, the content of Pouzolzia zeylanica extract is 5wt%-10wt%, the content of vitamin E is 1wt%-5wt%, and the balance is pharmaceutically acceptable excipients.

[0005] Further, the specific preparation process of the Pouzolzia zeylanica extract is as follows: The Pouzolzia zeylanica raw material is cleaned and dried, and then extracted by ethanol reflux extraction method. Then the extract is filtered and concentrated to obtain the Pouzolzia zeylanica extract.

[0006] Further, the pharmaceutically acceptable excipients include sodium hyaluronate, glyceryl oleate, cetearyl alcohol, glyceryl stearate, glycerol, butanediol, xanthan gum, methyl glucoside sesquistearate and deionized water.

[0007] Further, the skin whitening compound preparation is composed of the following raw materials in weight percentages: 5wt%-10wt% of Pouzolzia zeylanica extract, 1wt%-5wt% of vitamin E, 0.04wt% of sodium hyaluronate, 4wt% of glyceryl oleate, 3.5wt% of cetearyl alcohol, 1.5wt% of glyceryl stearate, 7wt% of glycerol, 1wt% of butanediol, 1.5wt% of xanthan gum and 3.5wt% of methyl glucoside sesquistearate, and the balance is deionized water.

[0008] The preparation method of the skin-whitening compound preparation described in the present invention is as follows: Mix 5wt%-10wt% of Pouzolzia zeylanica extract with 1wt%-5wt% of vitamin E, then add 0.04wt% of sodium hyaluronate, 4wt% of glyceride, 3.5wt% of cetearyl alcohol, 1.5wt% of glyceryl stearate, 7wt% of glycerol, 1wt% of butanediol, 1.5wt% of xanthan gum, and 3.5wt% of methyl glucoside sesquistearate. Make up to 100% with deionized water, stir and mix evenly to obtain an emulsion preparation, and keep the pH value at 5.5-6.5 to obtain the product skin-whitening compound preparation.

[0009] The application of the skin-whitening compound preparation described in the present invention as a whitening skin care product is as follows: Apply the skin-whitening compound preparation on the skin surface, 1-2 times a day, and continuously use it for 4-8 weeks, which can significantly improve the skin color uniformity and at the same time improve the skin glossiness. Both Pouzolzia zeylanica and vitamin E are natural ingredients, with wide sources, high safety, little irritation to the skin, and no harm to the human body after long-term use.

[0010] Compared with the prior art, the present invention has the following advantages and beneficial effects: The compound preparation based on Pouzolzia zeylanica and vitamin E prepared by the present invention has a good effect on skin whitening. Pouzolzia zeylanica is the whole herb with roots of the Urticaceae plant, which has the functions of antioxidation, anti-inflammation and regulating lipid metabolism. As a powerful antioxidant, vitamin E can protect the skin from free radical damage and promote the stability and repair of the skin. Through the synergistic effect of Pouzolzia zeylanica extract and vitamin E, this compound preparation can inhibit the activity of tyrosinase, thereby inhibiting the generation of melanin, improving the skin color uniformity, and increasing the water content of the skin with a moisturizing function. The compound preparation of the present invention is safe and non-irritating, suitable for long-term use, and can be widely applied to the fields of cosmetics or medicine. Specific embodiments

[0011] The following further elaborates on the above content of the present invention through examples, but this should not be construed as limiting the scope of the above subject matter of the present invention to the following examples. Any technology implemented based on the above content of the present invention belongs to the scope of the present invention.

[0012] Composition of the compound preparation:

[0013] The compound preparation is specifically composed of the following raw materials in weight percentages: 8wt% of Pouzolzia zeylanica extract, 3wt% of vitamin E, 0.04wt% of sodium hyaluronate, 4wt% of glyceride, 3.5wt% of cetearyl alcohol, 1.5wt% of glyceryl stearate, 7wt% of glycerol, 1wt% of butanediol, 1.5wt% of xanthan gum, and 3.5wt% of methyl glucoside sesquistearate, with the balance being deionized water.

[0014] Extraction of Pouzolzia zeylanica extract:

[0015] Select high-quality Pouzolzia zeylanica raw materials. After cleaning and drying, use the ethanol reflux extraction method for extraction. Filter and concentrate the extract to obtain the Pouzolzia zeylanica extract.

[0016] Preparation method of the compound preparation:

[0017] Mix 8 wt% of the Pouzolzia zeylanica extract with 3 wt% of vitamin E, then add 0.04 wt% of sodium hyaluronate, 4 wt% of glyceryl ester, 3.5 wt% of cetearyl alcohol, 1.5 wt% of glyceryl stearate, 7 wt% of glycerol, 1 wt% of butanediol, 1.5 wt% of xanthan gum and 3.5 wt% of methyl glucoside sesquistearate, and make up to 100% with deionized water. Stir and mix evenly to obtain an emulsion preparation, and keep the pH value at 5.5 - 6.5 to obtain the skin-whitening compound preparation of the product.

[0018] Usage method of the compound preparation:

[0019] Apply this compound preparation to the skin surface 1 - 2 times a day for 4 - 8 consecutive weeks, which can significantly improve the skin color uniformity and skin gloss. Both Pouzolzia zeylanica and vitamin E are natural ingredients, with wide sources, high safety, little irritation to the skin, and no harm to the human body after long-term use.

[0020] 1. Melanin reduction rate

[0021] Select 30 healthy women aged 25 - 50 with dull skin color, randomly numbered and divided into 6 groups, with 5 people in each group. After the skin of each group of subjects is cleaned with warm water, apply the compound preparation of Pouzolzia zeylanica and vitamin E to the face, once in the morning and once in the evening every day for 4 weeks. During the experiment, the subjects are not allowed to use any other skin care products. Before use, on the 7th day, 14th day, 21st day, and 28th day, use the skin melanin and heme tester MX18 to measure the skin surface of the subjects, and take the average value after measuring 3 times. Test result: Skin melanin content reduction rate = MI Tn -MI T0 / MI T0 *100%, where MI Tn--- Tn value of the test area; MI T0--- Initial value of the test area.

[0022] 2. Inhibitory effect on tyrosinase activity

[0023] Add 50 μL of L-DOPA solution and 50 μL of the compound preparation of Pouzolzia zeylanica with different concentrations and tyrosinase solution to a 96-well plate; after mixing evenly, place it in a water bath at 30 °C for reaction for 25 min; then measure the absorbance A (490 nm). Each group of experiments is repeated 3 times, and the absorbance of each group of experiments is the average value of 3 measurements; calculate the tyrosinase inhibition rate;

[0024] The calculation formula for tyrosinase inhibition rate is: Tyrosinase inhibition rate = [1 - (A x1 - A x2 ), / (A Y1 - A Y2 )] * 100%;

[0025] Among them, A x1 is the absorbance measured when adding tyrosinase solution and the sample solution to be tested;

[0026] A x2 is the absorbance measured when not adding tyrosinase solution (replaced with an equal amount of phosphate buffer solution) but adding the compound preparation of Pouzolzia zeylanica;

[0027] A Y1 is the absorbance measured when adding tyrosinase solution but not adding the compound preparation of Pouzolzia zeylanica (replaced with an equal amount of phosphate buffer solution);

[0028] A Y2 is the absorbance measured when neither adding tyrosinase solution nor adding the compound preparation of Pouzolzia zeylanica (replaced with an equal amount of phosphate buffer solution).

[0029] In the above experiment, the concentration of L - dopa solution is 1 g / L, which is prepared with a 0.2 mol / L phosphate buffer solution with a pH of 6.8; different concentrations of the compound preparation of Pouzolzia zeylanica are prepared with a 0.2 mol / L phosphate buffer solution with a pH of 6.8: the concentration of tyrosinase solution is 100 U / mL, which is prepared with a 0.2 mol / L phosphate buffer solution with a pH of 6.8.

[0030] Moisturizing effect detection

[0031] Before use, on the 7th day, 14th day, 21st day, and 28th day, use the moisture tester Cormeometer CM825 to measure the water content of the stratum corneum at the highest point of the right cheek of each subject. Use the point - touch measurement method and take the average value after measuring 3 times. Before the test, the subjects wash their faces and rest quietly for 30 min. Test result: Skin moisture content growth rate % = (MMV t - MMV0) / MMV0 * 100%; MMV0: Skin water content before use; MMV t : Skin water content after t days of use.

[0032] Results

[0033] 1. Melanin reduction rate

[0034] Table 1 Melanin reduction rate

[0035]

[0036] Remark: p < 0.05

[0037] As can be seen from Table 1, for the six groups of people, the melanin significantly decreased within 28 days when using the product of the present application, and the reduction rate was positively correlated with the usage time.

[0038] 2. Tyrosinase activity inhibition rate

[0039] Table 2 Tyrosinase activity inhibition rate

[0040] Concentration of Pouzolzia zeylanica complex preparation (g / L) Tyrosinase inhibition rate (%) P value SD 0.3 28.87 ＜0.05 8.56 0.5 33.25 ＜0.05 6.89 0.7 36.89 ＜0.05 5.97

[0041] From the experimental results in Table 2, it can be seen that the product of the present application has an inhibitory effect on tyrosinase and can more effectively reduce the amount of melanin production.

[0042] 3. Skin moisture content growth rate

[0043] Table 3 Moisture content growth rate

[0044]

[0045] Remark: P < 0.05

[0046] From the experimental results in Table 3, it can be seen that for the six groups of people, the skin moisture significantly increased within 28 days when using the product of the present application, and the growth rate was positively correlated with the usage time.

[0047] The above shows and describes the basic principles, main features and advantages of the present invention. Without departing from the spirit and scope of the present invention, the present invention has various changes and improvements, and these changes and improvements all fall within the scope of the present invention claimed.

Claims

1. A skin-whitening compound preparation, characterized in that The skin-whitening compound preparation mainly consists of Pouzolzia zeylanica extract and vitamin E, wherein the content of Pouzolzia zeylanica extract is 5wt%-10wt%, the content of vitamin E is 1wt%-5wt%, and the balance is pharmaceutically acceptable excipients.

2. The skin-whitening compound preparation according to claim 1, wherein The specific preparation process of the Pouzolzia zeylanica extract is as follows: The Pouzolzia zeylanica raw material is cleaned and dried, and then extracted by ethanol reflux extraction method. The extract is filtered and concentrated to obtain the Pouzolzia zeylanica extract.

3. The skin-whitening compound preparation according to claim 1, characterized in that: The pharmaceutically acceptable excipients include sodium hyaluronate, glyceryl oleate, cetearyl alcohol, glyceryl stearate, glycerol, butanediol, xanthan gum, methyl glucoside sesquistearate and deionized water.

4. The skin-whitening compound preparation according to claim 1, characterized in that The skin-whitening compound preparation is composed of the following raw materials in weight percentages: 5wt%-10wt% of Pouzolzia zeylanica extract, 1wt%-5wt% of vitamin E, 0.04wt% of sodium hyaluronate, 4wt% of glyceryl oleate, 3.5wt% of cetearyl alcohol, 1.5wt% of glyceryl stearate, 7wt% of glycerol, 1wt% of butanediol, 1.5wt% of xanthan gum and 3.5wt% of methyl glucoside sesquistearate, and the balance is deionized water.

5. The preparation method of the skin-whitening compound preparation according to any one of claims 1-4, characterized in that The specific preparation process is as follows: 5wt%-10wt% of Pouzolzia zeylanica extract is mixed with 1wt%-5wt% of vitamin E, and then 0.04wt% of sodium hyaluronate, 4wt% of glyceryl oleate, 3.5wt% of cetearyl alcohol, 1.5wt% of glyceryl stearate, 7wt% of glycerol, 1wt% of butanediol, 1.5wt% of xanthan gum and 3.5wt% of methyl glucoside sesquistearate are added. Deionized water is added to make up 100%. The mixture is stirred and mixed evenly to obtain an emulsion preparation, and the pH value is maintained at 5.5-6.5 to obtain the product skin-whitening compound preparation.

6. Use of the skin-whitening compound preparation according to any one of claims 1-4 as a whitening skin care product.

7. The application according to claim 6, wherein The specific use process is as follows: The skin-whitening compound preparation is applied to the skin surface 1-2 times a day for 4-8 consecutive weeks, which can significantly improve the skin color uniformity and at the same time improve the skin gloss.

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