Anti-inflammatory and acne-removing traditional Chinese medicine composition and application thereof

Abstract

The invention discloses an anti-inflammatory and acne-removing traditional Chinese medicine composition and application thereof.The anti-inflammatory and acne-removing traditional Chinese medicine composition is prepared by wrapping a traditional Chinese medicine extract with a cell penetrating peptide modified nano-carrier, and the traditional Chinese medicine extract is extracted from, by mass, 35-45 parts of cottonrose hibiscus leaves, 10-20 parts of sanguisorba officinalis, 10-15 parts of ovateleaf holly bark and 10-15 parts of peach kernels. , 5-10 parts of Indian trumetflower seed, 5-10 parts of dahurian angelica root, 5-8 parts of licorice root, and 1-5 parts of mint leaf. The anti-inflammatory and acne-removing traditional Chinese medicine composition is compounded according to the monarch, minister, assistant and guide theory of traditional Chinese medicine, has a remarkable killing effect on propionibacterium and has a remarkable synergistic effect on anti-inflammatory and acne-removing effects; the traditional Chinese medicine extract is wrapped by the cell-penetrating peptide modified nano-carrier, so that the stability of active ingredients of the traditional Chinese medicine extract and the effect of the traditional Chinese medicine on the skin are improved.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and particularly relates to an anti-inflammatory and anti-acne traditional Chinese medicine composition and its application. Background Art

[0002] With the increasing awareness of consumers about the safety of using skin care products, consumers have learned that chemically synthesized ingredients are likely to cause new acne problems, and thus are more inclined to choose acne treatment products prepared with mild and non-irritating natural ingredients. Among them, traditional Chinese medicine acne treatment ingredients, as the main natural ingredients, have gradually been developed and applied to skin care products. However, compared with chemically synthesized ingredients such as salicylic acid and nicotinamide that directly act on the skin surface, the ingredients of traditional Chinese medicine extracts are more complex, and multiple ingredients need to work together to take effect. Moreover, there are ingredients with poor stability, and it is necessary to sacrifice permeability to maintain activity, resulting in a longer effective period for traditional Chinese medicine ingredients compared with chemically synthesized ingredients. Summary of the Invention

[0003] The present invention provides an anti-inflammatory and anti-acne traditional Chinese medicine composition and its application to solve the technical problem of the long effective period of traditional Chinese medicine extract ingredients.

[0004] To solve the above technical problem, on the one hand, the present invention provides an anti-inflammatory and anti-acne traditional Chinese medicine composition, which is prepared by wrapping a traditional Chinese medicine extract with a nano-carrier modified by a cell-penetrating peptide. The traditional Chinese medicine extract is obtained by extracting the following traditional Chinese medicine raw materials according to mass parts:

[0005] 35 to 45 parts of Hibiscus mutabilis leaf, 10 to 20 parts of Sanguisorba officinalis, 10 to 15 parts of Ilex rotunda, 10 to 15 parts of Prunus persica, 5 to 10 parts of Oroxylum indicum, 5 to 10 parts of Angelica dahurica, 5 to 8 parts of Glycyrrhiza uralensis, 1 to 5 parts of Mentha haplocalyx Briq.

[0006] The Hibiscus mutabilis leaf is the leaf of Hibiscus mutabilis L. of the Malvaceae family. It has a pungent taste and a neutral nature, and belongs to the lung and liver meridians. It has the effects of clearing heat and detoxifying, cooling blood and dissipating carbuncles, and is mainly used to treat carbuncles, throat swelling, snake sores around the body, burns, red and swollen eyes, and traumatic injuries.

[0007] Sanguisorba officinalis is a perennial herb of the genus Sanguisorba of the Rosaceae family. It has a bitter, sour and astringent taste and a cold nature, and belongs to the liver and large intestine meridians. It has the effects of cooling blood and stopping bleeding, detoxifying and astringing sores, and can be used to treat hematochezia, hemorrhoids, dysentery, metrorrhagia and metrostaxis, scalds and burns, carbuncles and sores.

[0008] Ilex rotunda is the bark or root bark of Ilex rotunda Thunb. of the Aquifoliaceae family. It has a bitter taste and a cold nature, and has the effects of clearing heat and detoxifying, promoting diuresis and relieving pain. It can be used to treat cold and fever, tonsillitis, sore throat, acute and chronic hepatitis, acute gastroenteritis, gastric and duodenal ulcers, rheumatic arthralgia, traumatic injuries, and burns.

[0009] Peach kernel is the dried and mature seed of Prunus persica or Prunus davidiana of the Rosaceae family. It is bitter, sweet, and neutral in nature, and belongs to the heart, liver, and large intestine meridians. It has the effects of promoting blood circulation to remove blood stasis, moistening the intestines to relieve constipation, and relieving cough and asthma. It can treat amenorrhea and dysmenorrhea, abdominal masses, lung abscess, intestinal abscess, traumatic injury, intestinal dryness and constipation, cough and asthma.

[0010] Oroxylum indicum is an upright small tree plant of the genus Oroxylum in the Bignoniaceae family. It is bitter, sweet, and cool in nature, and belongs to the lung, liver, and stomach meridians. It has the effects of clearing the lung, relieving sore throat and cough, soothing the liver and regulating the stomach. It can treat lung heat cough, throat obstruction, hoarseness, liver and stomach qi pain, and non-healing of sore openings.

[0011] Angelica dahurica is a perennial tall herbaceous plant of the genus Angelica in the Apiaceae family. It is pungent in taste, warm in nature, and belongs to the stomach, large intestine, and lung meridians. It has the effects of expelling cold and relieving pain, dredging nasal congestion, regulating secretions, and promoting the healing of sore openings. It can treat cold headache, nasal congestion and runny nose, toothache, and skin swelling and redness.

[0012] Licorice is a perennial herbaceous plant of the genus Glycyrrhiza in the Fabaceae family. It is sweet in taste, neutral in nature, and belongs to the heart, lung, spleen, and stomach meridians. It has the effects of replenishing qi, detoxifying, and harmonizing various herbs. It can treat shortness of breath, abdominal pain, and food poisoning.

[0013] Peppermint leaves are the leaves of Mentha haplocalyx Briq. of the genus Mentha in the Lamiaceae family. It is pungent in taste, cool in nature, and belongs to the lung and liver meridians. It has the effects of dispersing wind-heat, clearing the head and eyes, relieving sore throat and promoting eruption, and soothing the liver and promoting qi movement. It can treat exogenous wind-heat, headache, sore throat, food stagnation and qi distension, mouth ulcers, toothache, sores and boils, urticaria, initial stage of febrile diseases, rubella and itching, liver qi stagnation, and chest and hypochondrium pain.

[0014] In some of these embodiments, the anti-inflammatory and acne-removing traditional Chinese medicine composition is prepared by the following steps:

[0015] Mix the traditional Chinese medicine extract, lipid film, and vegetable oil, and then add a surfactant and a co-surfactant, and stir well to obtain a mixture;

[0016] Perform multiple cycles of homogenization on the mixture through a high-pressure homogenizer, with the homogenization pressure being 1000 bar to 2000 bar, until the particle size of the mixture is 10 nm to 100 nm, to obtain a lipid nanoemulsion;

[0017] Add the activated cell-penetrating peptide to the lipid nanoemulsion, stir at room temperature for 8 h to 12 h, then wash and centrifuge to obtain the anti-inflammatory and acne-removing traditional Chinese medicine composition.

[0018] The above lipid film is a film obtained by dissolving phospholipids and cholesterol in chloroform and rotary evaporating; the above vegetable oils include camellia seed oil, grape seed oil, macadamia nut oil, rosehip oil, sea buckthorn oil, and baobab oil, etc.; the above surfactants include lecithin, Tween 20, Tween 80, Span 80, Brij 35, and Brij 98, etc.; the co-surfactants include ethanol, propylene glycol, glycerol, and polyethylene glycol, etc.

[0019] In some of these embodiments, the traditional Chinese medicine extract is obtained by the following steps:

[0020] Weigh hibiscus leaf, sanguisorba root, ilicis rotundatae folium, peach kernel, oroxylum indicum and liquorice by mass parts, wash, dry and pulverize them, add an ethanol solution with a concentration of 50%, perform ultrasonic treatment at normal temperature for 30 min to 60 min, with an ultrasonic power of 300 W to 800 W, remove the supernatant, centrifuge, filter and reflux the ethanol to obtain extract A;

[0021] Weigh angelica dahurica and mint by mass parts, add an ethanol solution with a concentration of 90%, perform ethanol reflux extraction at 60°C to 80°C for 2 h to 3 h, and filter to obtain extract B;

[0022] Mix extract A and extract B evenly to obtain the traditional Chinese medicine extract.

[0023] In some of these embodiments, the mass ratio of sanguisorba root to ilicis rotundatae folium is 1:(0.6 - 1.2), which can prevent the skin from showing compensatory oiliness.

[0024] In some of these embodiments, the traditional Chinese medicine extract is obtained from the following traditional Chinese medicine raw materials by mass parts:

[0025] 38 parts of hibiscus leaf, 10 parts of sanguisorba root, 12 parts of ilicis rotundatae folium, 10 parts of peach kernel, 10 parts of oroxylum indicum, 5 parts of angelica dahurica, 8 parts of liquorice, 1 part of mint.

[0026] In some of these embodiments, the traditional Chinese medicine extract is obtained from the following traditional Chinese medicine raw materials by mass parts:

[0027] 45 parts of hibiscus leaf, 18 parts of sanguisorba root, 15 parts of ilicis rotundatae folium, 10 parts of peach kernel, 5 parts of oroxylum indicum, 8 parts of angelica dahurica, 5 parts of liquorice, 3 parts of mint.

[0028] In some of these embodiments, the cell-penetrating peptide includes one or more of TAT peptide, Penetratin peptide and Polyarginine peptide.

[0029] In some of these embodiments, the nanocarrier includes liposome and nanoemulsion.

[0030] The present invention also provides an application of an anti-inflammatory and anti-acne traditional Chinese medicine composition in the preparation of cosmetics, and the cosmetics include facial mask, facial cleanser, essence or cream.

[0031] Compared with the prior art, the present invention has the following beneficial effects:

[0032] The present invention formulates an anti-inflammatory and acne-removing traditional Chinese medicine composition according to the theory of monarch, minister, assistant, and guide in traditional Chinese medicine. With Hibiscus mutabilis leaf as the monarch drug, aiming at the core pathogenesis of acne, "stagnation of heat toxin", it fundamentally improves skin inflammation and enhances the skin's anti-inflammatory and detoxifying ability; Sanguisorba officinalis and Boehmeria nivea var. tenacissima are used as ministerial drugs to assist the monarch drug in strengthening the anti-inflammatory and detoxifying effects, improving capillary permeability, and reducing inflammatory exudation; Prunus persica and Oroxylum indicum are used as assistant drugs to promote blood circulation and remove blood stasis, improve blood circulation between the skin pores and muscles, promote the metabolism of inflammatory substances, and assist the ministerial drugs in enhancing the skin's detoxifying ability; Angelica dahurica, Glycyrrhiza glabra, and Mentha haplocalyx are used as guiding drugs to harmonize various drugs, direct the medicinal effects to reach the skin pores and muscles, and keep the skin cool and soothed, which conforms to the acne treatment principle of "treating heat toxin as the root and regulating qi and blood as the application". The anti-inflammatory and acne-removing traditional Chinese medicine composition of the present invention has a significant bactericidal effect on Propionibacterium acnes and has a significant synergistic effect in anti-inflammatory and acne-removing.

[0033] Meanwhile, the present invention uses a nanocarrier modified with a cell-penetrating peptide to encapsulate the traditional Chinese medicine extract, uses the nanocarrier to protect the traditional Chinese medicine extract, improves the stability of the active ingredients of the traditional Chinese medicine extract, and uses the cell-penetrating peptide to guide the active ingredients of the traditional Chinese medicine to reach the cells directly, thereby improving the effect of the traditional Chinese medicine on the skin. Detailed implementation manners

[0034] To make the above objects, features, and advantages of the present invention more obvious and understandable, the following provides a detailed description of the specific implementation manners of the present invention. Many specific details are set forth in the following description in order to fully understand the present invention. However, the present invention can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without departing from the connotation of the present invention. Therefore, the present invention is not limited by the specific embodiments disclosed below.

[0035] As used herein, the term "prepared from" is synonymous with "comprising". The terms "comprising", "including", "having", "containing" or any other variation thereof used herein are intended to cover non-exclusive inclusion. For example, a composition, step, method, article, or device containing the listed elements is not necessarily limited to those elements, but may include other elements not expressly listed or elements inherent to such composition, step, method, article, or device.

[0036] When an equivalent, concentration, or other value or parameter is expressed as a range, a preferred range, or a range defined by a series of upper preferred values and lower preferred values, it should be understood that all ranges formed by any pairing of any range upper limit or preferred value with any range lower limit or preferred value are specifically disclosed, regardless of whether the ranges are separately disclosed. For example, when the range "1 to 5" is disclosed, the described range should be interpreted to include the ranges "1 to 4", "1 to 3", "1 to 2", "1 to 2 and 4 to 5", "1 to 3 and 5", etc. When a numerical range is described herein, unless otherwise stated, the range is intended to include its end values and all integers and fractions within the range.

[0037] In addition, the indefinite articles "a" and "an" before the elements or components of the present invention do not limit the quantity requirements (i.e., the number of occurrences) of the elements or components. Therefore, "a" or "an" should be construed as including one or at least one, and the singular form of the elements or components also includes the plural form, unless the quantity clearly refers to the singular form.

[0038] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the technical field to which the present invention pertains. The terms used herein in the specification of the present invention are for the purpose of describing specific embodiments only and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more of the related listed items.

[0039] Example 1

[0040] An anti-inflammatory and acne-removing traditional Chinese medicine composition is prepared by encapsulating a traditional Chinese medicine extract with a nanocarrier modified by a cell-penetrating peptide. The traditional Chinese medicine extract is obtained by extracting the following traditional Chinese medicine raw materials by mass parts:

[0041] 35 parts of Abelmoschus manihot leaves, 10 parts of Sanguisorba officinalis, 10 parts of Ilex rotunda, 10 parts of Prunus persica, 5 parts of Oroxylum indicum, 5 parts of Angelica dahurica, 5 parts of Glycyrrhiza uralensis, and 1 part of Mentha haplocalyx Briq.

[0042] The anti-inflammatory and acne-removing traditional Chinese medicine composition is prepared by the following steps:

[0043] Weigh Abelmoschus manihot leaves, Sanguisorba officinalis, Ilex rotunda, Prunus persica, Oroxylum indicum and Glycyrrhiza uralensis by mass parts, wash, dry, and pulverize them, add an ethanol solution with a concentration of 50%, perform ultrasonic treatment at room temperature for 30 min, with an ultrasonic power of 800 W, remove the supernatant, centrifuge, filter, and reflux the ethanol to obtain extract A;

[0044] Weigh Angelica dahurica and Mentha haplocalyx Briq. by mass parts, add an ethanol solution with a concentration of 90%, perform ethanol reflux extraction at 60 °C for 3 h, and filter to obtain extract B;

[0045] Mix extract A and extract B evenly to obtain a traditional Chinese medicine extract;

[0046] Weigh 20% of the traditional Chinese medicine extract, 40% of a lipid film (phospholipid:cholesterol = 4:1), 20% of vegetable oil, 10% of surfactant, 8% of co-surfactant, and 2% of cell-penetrating peptide by mass percentage. Mix the traditional Chinese medicine extract, lipid film, and vegetable oil, then add the surfactant and co-surfactant, and stir well to obtain a mixture;

[0047] Perform multiple cycles of homogenization on the mixture with a high-pressure homogenizer at a homogenization pressure of 2000 bar until the particle size of the mixture is 10 nm to obtain a lipid nanoemulsion;

[0048] Add 2% of the activated cell-penetrating peptide to the lipid nanoemulsion, stir at room temperature for 8 h, wash and centrifuge to obtain the anti-inflammatory and acne-removing traditional Chinese medicine composition.

[0049] Example 2

[0050] An anti-inflammatory and acne-removing traditional Chinese medicine composition is prepared by encapsulating a traditional Chinese medicine extract with a nano-carrier modified by a cell-penetrating peptide. The traditional Chinese medicine extract is obtained by extracting the following traditional Chinese medicine raw materials according to mass parts:

[0051] 45 parts of Hibiscus mutabilis leaf, 20 parts of Sanguisorba officinalis, 15 parts of Ilex rotunda, 15 parts of Prunus persica, 10 parts of Oroxylum indicum, 10 parts of Angelica dahurica, 8 parts of Glycyrrhiza glabra, 5 parts of Mentha haplocalyx Briq.

[0052] The anti-inflammatory and acne-removing traditional Chinese medicine composition is prepared by the following steps:

[0053] Weigh Hibiscus mutabilis leaf, Sanguisorba officinalis, Ilex rotunda, Prunus persica, Oroxylum indicum and Glycyrrhiza glabra according to mass parts, clean, dry and pulverize them, add an ethanol solution with a concentration of 50%, perform ultrasonic treatment at normal temperature for 60 min, with an ultrasonic power of 300 W, remove the supernatant, centrifuge, filter and reflux ethanol to obtain extract A;

[0054] Weigh Angelica dahurica and Mentha haplocalyx Briq. according to mass parts, add an ethanol solution with a concentration of 90%, perform ethanol reflux extraction at 80 °C for 2 h, and filter to obtain extract B;

[0055] Mix extract A and extract B evenly to obtain the traditional Chinese medicine extract;

[0056] Weigh 20% of the traditional Chinese medicine extract, 40% of the lipid film (phospholipid:cholesterol = 4:1), 20% of vegetable oil, 10% of surfactant, 8% of co-surfactant and 2% of cell-penetrating peptide according to mass percentage. Mix the traditional Chinese medicine extract, lipid film and vegetable oil, then add the surfactant and co-surfactant, and stir well to obtain a mixed solution;

[0057] Perform multiple cycles of homogenization on the mixed solution through a high-pressure homogenizer, with a homogenization pressure of 1000 bar until the particle size of the mixture is 100 nm to obtain the lipid nanoemulsion;

[0058] Add the activated cell-penetrating peptide to the lipid nanoemulsion, stir at room temperature for 12 h, wash and centrifuge to obtain the anti-inflammatory and acne-removing traditional Chinese medicine composition.

[0059] Example 3

[0060] An anti-inflammatory and acne-removing traditional Chinese medicine composition is prepared by encapsulating a traditional Chinese medicine extract with a nano-carrier modified by a cell-penetrating peptide. The traditional Chinese medicine extract is obtained by extracting the following traditional Chinese medicine raw materials according to mass parts:

[0061] 38 parts of hibiscus mutabilis leaf, 10 parts of sanguisorba officinalis, 12 parts of ilicistriloba hook.f., 10 parts of semen sterculiae lychnophorae, 10 parts of oroxylum indicum, 5 parts of angelica dahurica, 8 parts of glycyrrhiza uralensis, 1 part of mentha haplocalyx briq.

[0062] The anti-inflammatory and acne-removing traditional Chinese medicine composition is prepared by the following steps:

[0063] Weigh hibiscus mutabilis leaf, sanguisorba officinalis, ilicistriloba hook.f., semen sterculiae lychnophorae, oroxylum indicum and glycyrrhiza uralensis according to the parts by mass, wash, dry and crush them, add an ethanol solution with a concentration of 50%, perform ultrasonic treatment at room temperature for 45 min, with an ultrasonic power of 550 W, remove the supernatant, centrifuge, filter and reflux the ethanol to obtain extract A;

[0064] Weigh angelica dahurica and mentha haplocalyx briq. according to the parts by mass, add an ethanol solution with a concentration of 90%, perform ethanol reflux extraction at 70 °C for 2.5 h, and filter to obtain extract B;

[0065] Mix extract A and extract B evenly to obtain a traditional Chinese medicine extract;

[0066] Weigh 20% of the traditional Chinese medicine extract, 40% of the lipid film (phospholipid:cholesterol = 4:1), 20% of vegetable oil, 10% of surfactant, 8% of co-surfactant and 2% of cell-penetrating peptide according to the mass percentage. Mix the traditional Chinese medicine extract, lipid film and vegetable oil, then add the surfactant and co-surfactant, and stir well to obtain a mixture;

[0067] Perform multiple cycles of homogenization on the mixture through a high-pressure homogenizer, with a homogenization pressure of 1500 bar until the particle size of the mixture is 30 nm to obtain lipid nanoemulsion;

[0068] Add the activated cell-penetrating peptide to the lipid nanoemulsion, stir at room temperature for 10 h, then wash and centrifuge to obtain the anti-inflammatory and acne-removing traditional Chinese medicine composition.

[0069] Example 4

[0070] An anti-inflammatory and acne-removing traditional Chinese medicine composition is prepared by wrapping a traditional Chinese medicine extract with a nano-carrier modified by a cell-penetrating peptide. The traditional Chinese medicine extract is obtained by extracting the following traditional Chinese medicine raw materials according to the parts by mass:

[0071] 44 parts of hibiscus mutabilis leaf, 18 parts of sanguisorba officinalis, 15 parts of ilicistriloba hook.f., 10 parts of semen sterculiae lychnophorae, 5 parts of oroxylum indicum, 8 parts of angelica dahurica, 5 parts of glycyrrhiza uralensis, 3 parts of mentha haplocalyx briq. The preparation steps of the anti-inflammatory and acne-removing traditional Chinese medicine composition are the same as those in Example 3.

[0072] Comparative Example 1: The difference from Example 3 is that hibiscus mutabilis leaf is not added.

[0073] Comparative Example 2: The difference from Example 3 is that sanguisorba officinalis and ilicistriloba hook.f. are not added.

[0074] Comparative Example 3: The difference from Example 3 is that peach kernels and oroxylum seeds are not added.

[0075] Comparative Example 4: The difference from Example 3 is that angelica dahurica, liquorice and peppermint leaves are not added.

[0076] Comparative Example 5: The difference from Example 3 is that the nanocarrier is not modified with cell-penetrating peptides and the cell-penetrating peptides are replaced with surfactants.

[0077] Comparative Example 6: The difference from Example 3 is that the traditional Chinese medicine extract is not encapsulated by the nanocarrier but directly used as an anti-inflammatory and anti-acne traditional Chinese medicine composition, and the dosage of the traditional Chinese medicine extract is the dosage corresponding to 20% in Example 3.

[0078] (I) Test of Propionibacterium acnes killing rate

[0079] Test samples: Examples 1 to 4 and Comparative Examples 1 to 6.

[0080] Test steps: Using the ATCC 6919 standard strain, culture in BHI agar supplemented with 5% defibrinated sheep blood, 1% vitamin K1 and 0.1% sodium thioglycollate in a 37°C anaerobic jar (85% N2, 10% CO2, 5% H2) for 48 hours, and adjust the bacterial suspension to 0.5 McFarland turbidity (about 1 - 5×10 8 CFU / mL); after sterilizing the test samples through a 0.22 μm filter membrane, take 100 μL of the bacterial suspension and mix it with 900 μL of the medicinal liquid for 6 h, and add D / E neutralizing solution containing 3% Tween 80 + 0.3% lecithin to terminate the reaction; take 100 μL of the mixed solution and dilute it in gradients (10 -1 to 10 -6 ), coat it on a pre-reduced CDC anaerobic blood agar plate, and culture anaerobically at 37°C for 72 hours, and count the number of colonies CFU. The Propionibacterium acnes killing rate % = (CFU in the control group - CFU in the experimental group) / CFU in the control group, and the test results are shown in Table 1.

[0081] (II) 5α-reductase inhibition rate

[0082] Test samples: Examples 1 to 4 and Comparative Examples 1 to 6.

[0083] Test procedure: Add a blank control solution (100 μL of 0.5 mg / mL testosterone, 200 μL of phosphate buffer solution, 15 μL of 1 mg / mL reduced coenzyme II tetrasodium salt solution) to the reaction tube, then add 15 μL of 150 mg / mL 5α-reductase, mix and incubate at 37 °C. After 60 min, measure the absorbance value at 340 nm (blank control sample A1). Add the blank control solution and 50 μL of 0.1% finasteride solution to the reaction tube, and finally add 15 μL of 150 mg / mL 5α-reductase, mix and incubate at 37 °C for 60 min, then measure the absorbance value at 340 nm (finasteride control sample A2). Add the blank control solution and 50 μL of the test sample to the reaction tube, then add 15 μL of 150 mg / mL 5α-reductase, mix and incubate at 37 °C for 60 min, and then measure the absorbance value at 340 nm (test sample A2). 5α-reductase inhibition rate The 5α-reductase inhibition rate is shown in Table 1 of the test results.

[0084] Table 1 Propionibacterium acnes killing rate and 5α-reductase inhibition rate

[0085] Propionibacterium acnes killing rate (%) 5α-reductase inhibition rate (%) Example 1 95.62 41.75 Example 2 98.41 47.54 Example 3 99.17 46.35 Example 4 99.85 49.42 Comparative Example 1 53.44 20.78 Comparative Example 2 60.86 9.29 Comparative Example 3 63.24 18.83 Comparative Example 4 64.57 14.46 Comparative Example 5 98.96 46.43 Comparative Example 6 99.14 46.11 Finasteride / 40.73

[0086] As can be seen from Table 2, the present invention uses the leaves of Hibiscus mutabilis, Sanguisorba officinalis, Ilex rotunda, Prunus persica, Oroxylum indicum, Angelica dahurica, Glycyrrhiza glabra and Mentha haplocalyx as the traditional Chinese medicine formula, which has a synergistic effect in anti-inflammatory sterilization and oil control and acne treatment. Among them, compared with Example 3, there is no significant difference in the Propionibacterium acnes killing rate and 5α-reductase inhibition rate in Comparative Example 5 and Comparative Example 6. It may be that the Propionibacterium acnes and 5α-reductase in vitro have no skin barrier to hinder the action of the anti-inflammatory and acne-removing traditional Chinese medicine composition.

[0087] (III) Volunteer test

[0088] Test samples: Sheet masks prepared from Examples 1 to 4 and Comparative Examples 1 to 6, and the mask components are shown in Table 2 by mass percentage.

[0089] Table 2 Content of sheet mask components

[0090]

[0091]

[0092] Test subjects: Volunteers with acne-prone skin aged 18 - 25 years old, and 5 people are assigned to each example or comparative example.

[0093] Test procedure:

[0094] 1. The subjects cleaned their faces with a commercially available amino acid facial cleanser product, dried their skin with a facial towel, and sat quietly in a waiting room at a temperature of 25 ± 1°C and a humidity of 50 ± 10% RH for more than 30 minutes; the average hemoglobin value in the acne area of the face was measured using a multi-functional 3D skin imaging analyzer, and the oil content in the T-zone of the face was tested 3 times with a skin oil detection probe, and the average value was taken.

[0095] 2. The subjects used a sheet mask once every night with the same procedure. On the 7th day, 14th day, and 28th day respectively, the average hemoglobin value in the acne area of the face was measured again using a multi-functional 3D skin imaging analyzer, and the oil content in the T-zone of the face was tested 3 times with a skin oil detection probe, and the average value was taken. Fill in the sensory evaluation, including irritation evaluation, acne treatment effect evaluation, oil control effect evaluation, and preference evaluation. Among them, the total score of each item is 10 points, and the higher the score, the lower the irritation, the more obvious the acne treatment effect, the more obvious the oil control effect, and the more favorite. Calculate the average score results of each sample, and the evaluation results are shown in Table 3.

[0096] 3. Calculate the hemoglobin improvement rate of each subject % = │average hemoglobin value on the nth day - average hemoglobin value on the 0th day│ / average hemoglobin value on the 0th day, and then calculate the average hemoglobin improvement rate of each sample according to the hemoglobin data of the subjects. The test results are shown in Table 4.

[0097] 4. Calculate the oil improvement rate of each subject % = │average oil content value on the nth day - average oil content value on the 0th day│ / average oil content value on the 0th day, and then calculate the average oil content improvement rate of each sample according to the oil data of the subjects. The test results are shown in Table 5.

[0098] Table 3 Sensory Use Evaluation

[0099]

[0100]

[0101] Table 4 Average Hemoglobin Improvement Rate

[0102] Sample Day 7 (%) Day 14 (%) Day 28 (%) Example 1 6.365 8.822 13.326 Example 2 9.143 12.217 17.742 Example 3 8.329 11.783 18.458 Example 4 9.012 12.149 18.753 Comparative Example 1 2.325 4.404 6.977 Comparative Example 2 3.196 5.528 8.724 Comparative Example 3 3.943 6.071 9.638 Comparative Example 4 4.393 7.842 7.125 Comparative Example 5 5.279 8.658 12.742 Comparative Example 6 4.840 7.549 11.048

[0103] Table 5 Average Oil Content Improvement Rate

[0104]

[0105]

[0106] As can be seen from Tables 3 to 5, Examples 1 to 4 are significantly superior to Comparative Examples 1 to 6 in terms of acne treatment effect and oil control effect. Among them, in Comparative Example 4, the medicinal agent was not added, and the average percentage change in hemoglobin decreased during the measurement on the 28th day. It may be that there was a rebound phenomenon in the acne-prone skin after the lack of the action of the medicinal agent.

[0107] The present invention compounded an anti-inflammatory and anti-acne traditional Chinese medicine composition in accordance with the theory of monarch, minister, assistant and guide in traditional Chinese medicine. With the leaf of Hibiscus mutabilis as the monarch drug, aiming at the core pathogenesis of acne, "stagnation of heat-toxin", it fundamentally improves skin inflammation and enhances the anti-inflammatory and detoxifying ability of the skin. Sanguisorba officinalis and Sambucus chinensis are used as ministerial drugs to assist the monarch drug in strengthening the anti-inflammatory and detoxifying effects, improving capillary permeability and reducing inflammatory exudation. Persica vulgaris and Oroxylum indicum are used as assistant drugs to promote blood circulation to remove blood stasis, improve blood circulation between the skin texture and muscles, promote the metabolism of inflammatory substances, and assist the ministerial drugs in enhancing the skin detoxification ability. Angelica dahurica, Glycyrrhiza glabra and Mentha haplocalyx are used as guiding drugs to harmonize all the drugs, direct the drug effect to the skin texture and muscles, and keep the skin cool and soothed, which conforms to the acne treatment principle of "clearing heat-toxin as the foundation and regulating qi and blood for application". The anti-inflammatory and anti-acne traditional Chinese medicine composition of the present invention has a significant bactericidal effect on Propionibacterium acnes and has a significant synergistic effect in anti-inflammatory and anti-acne.

[0108] At the same time, the present invention uses a cell-penetrating peptide-modified nanocarrier to encapsulate the traditional Chinese medicine extract, uses the nanocarrier to protect the traditional Chinese medicine extract, improves the stability of the active ingredients of the traditional Chinese medicine extract, and uses the cell-penetrating peptide to guide the active ingredients of the traditional Chinese medicine directly to the cells to improve the effect of the traditional Chinese medicine on the skin.

[0109] The technical features of the above-described embodiments can be combined arbitrarily. For the sake of concise description, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, it should be considered as the scope recorded in this specification.

[0110] The above-described specific embodiments have further elaborated on the purpose, technical solutions and beneficial effects of the present invention. It should be understood that the above is only the specific embodiments of the present invention and is not used to limit the protection scope of the present invention. In particular, it is pointed out that for those skilled in the art, any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. An anti-inflammatory and acne-removing traditional Chinese medicine composition, characterized in that, It is prepared by wrapping traditional Chinese medicine extract with a nanocarrier modified by a cell-penetrating peptide. The traditional Chinese medicine extract is obtained by extracting the following traditional Chinese medicine raw materials according to the mass parts: 35 to 45 parts of hibiscus leaf, 10 to 20 parts of sanguisorba root, 10 to 15 parts of ilicis rotundae cortex, 10 to 15 parts of peach kernel, 5 to 10 parts of oroxylum indicum seed, 5 to 10 parts of angelica dahurica root, 5 to 8 parts of liquorice root, 1 to 5 parts of mint leaf.

2. The anti-inflammatory and acne-removing traditional Chinese medicine composition according to claim 1, characterized in that The anti-inflammatory and acne-removing traditional Chinese medicine composition is prepared by the following steps: Mix the traditional Chinese medicine extract, lipid film and vegetable oil, then add a surfactant and a co-surfactant, and stir well to obtain a mixture; Perform multiple cycles of homogenization on the mixture by a high-pressure homogenizer at a homogenization pressure of 1000 bar to 2000 bar until the particle size of the mixture is 10 nm to 100 nm to obtain a lipid nanoemulsion; Add the activated cell-penetrating peptide to the lipid nanoemulsion, stir at room temperature for 8 h to 12 h, then wash and centrifuge to obtain the anti-inflammatory and acne-removing traditional Chinese medicine composition.

3. The anti-inflammatory and acne-removing traditional Chinese medicine composition according to claim 1, wherein The traditional Chinese medicine extract is obtained by the following steps: Weigh hibiscus leaf, sanguisorba root, ilicis rotundae cortex, peach kernel, oroxylum indicum seed and liquorice root according to the mass parts, clean, dry and crush them, add an ethanol solution with a concentration of 50%, perform ultrasonic treatment at room temperature for 30 min to 60 min, with an ultrasonic power of 300 W to 800 W, remove the supernatant, centrifuge, filter and reflux the ethanol to obtain extract A; Weigh angelica dahurica root and mint leaf according to the mass parts, add an ethanol solution with a concentration of 90%, perform ethanol reflux extraction at 60 °C to 80 °C for 2 h to 3 h, and filter to obtain extract B; Mix extract A and extract B evenly to obtain the traditional Chinese medicine extract.

4. The anti-inflammatory and acne-removing traditional Chinese medicine composition according to claim 1, wherein The mass ratio of sanguisorba root to ilicis rotundae cortex is 1:(0.6 to 1.2).

5. The anti-inflammatory and acne-removing traditional Chinese medicine composition according to claim 1, characterized in that, The traditional Chinese medicine extract is obtained by extracting the following traditional Chinese medicine raw materials according to the mass parts: 38 parts of hibiscus leaf, 10 parts of sanguisorba root, 12 parts of ilicis rotundae cortex, 10 parts of peach kernel, 10 parts of oroxylum indicum seed, 5 parts of angelica dahurica root, 8 parts of liquorice root, 1 part of mint leaf.

6. The anti-inflammatory and acne-removing traditional Chinese medicine composition according to claim 1, characterized in that, The traditional Chinese medicine extract is obtained by extracting the following traditional Chinese medicine raw materials according to the mass parts: 45 parts of hibiscus leaf, 18 parts of sanguisorba root, 15 parts of ilicis rotundae cortex, 10 parts of peach kernel, 5 parts of oroxylum indicum seed, 8 parts of angelica dahurica root, 5 parts of liquorice root, 3 parts of mint leaf.

7. The anti-inflammatory and acne-removing traditional Chinese medicine composition according to claim 1, wherein The cell-penetrating peptide includes one or more of TAT peptide, Penetratin peptide and Polyarginine peptide.

8. The anti-inflammatory and acne-removing traditional Chinese medicine composition according to claim 1, wherein The nanocarrier includes liposome and nanoemulsion.

9. Use of an anti-inflammatory and acne-removing traditional Chinese medicine composition according to any one of claims 1 to 8 in the preparation of cosmetics, characterized in that, The cosmetics include facial mask, facial cleanser, essence or cream.

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