A traditional Chinese medicine composition for preventing and treating inflammatory diseases of animals, a preparation method thereof and application thereof

Abstract

This invention discloses a traditional Chinese medicine composition for preventing and treating inflammatory diseases in animals, its preparation method, and its application, belonging to the field of traditional Chinese medicine composition technology. The raw materials of this traditional Chinese medicine composition for preventing and treating inflammatory diseases in animals, by mass percentage, include 8%-12% extract of honeysuckle branches and leaves, 8%-12% extract of dipsacus root, 8%-12% chrysanthemum, 4%-6% mulberry leaves, 4%-6% licorice root, 0.8%-1.2% peppermint, and 50.8%-67.2% anhydrous glucose. It has good anti-inflammatory effects and can effectively alleviate inflammatory responses caused by LPS. In particular, it has good therapeutic and ameliorative effects on the clinically prevalent porcine reproductive and respiratory syndrome (PRRS) and infectious bronchitis in chickens, effectively reducing morbidity and mortality, and improving production performance.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine composition technology, and more specifically, to a traditional Chinese medicine composition for preventing and treating inflammatory diseases in animals, its preparation method, and its application. Background Technology

[0002] During livestock and poultry farming, infections by pathogens such as bacteria, viruses, and parasites can lead to various infectious diseases. The proliferation of these pathogens in the animal's tissues and organs, along with the release of toxins, triggers the immune system, causing an immune response and resulting in a series of inflammatory symptoms. Acute inflammation can cause severe clinical symptoms and even death, while chronic inflammation leads to continuous nutritional depletion, reducing feed conversion ratios and economic benefits, as well as lowering the quality of livestock and poultry products and posing food safety risks. Most anti-inflammatory drugs used clinically are hormonal or non-steroidal anti-inflammatory drugs (NSAIDs), which have serious toxic side effects with long-term use and also pose food safety risks.

[0003] In view of this, the present invention is proposed. Summary of the Invention

[0004] The purpose of this invention is to provide a traditional Chinese medicine composition for the prevention and treatment of inflammatory diseases in animals, its preparation method, and its application.

[0005] This invention is implemented as follows:

[0006] In a first aspect, the present invention provides a traditional Chinese medicine composition for preventing and treating inflammatory diseases in animals, wherein the raw materials, by mass percentage, include 8%-12% extract of honeysuckle branches and leaves, 8%-12% extract of dipsacus root, 8%-12% chrysanthemum, 4%-6% mulberry leaves, 4%-6% licorice, 0.8%-1.2% peppermint, and 50.8%-67.2% anhydrous glucose.

[0007] In an optional embodiment, the raw materials, by mass percentage, include 9%-11% of honeysuckle twig and leaf extract, 9%-11% of Sichuan teasel root extract, 9%-11% of chrysanthemum, 4.5%-5.5% of mulberry leaf, 4.5%-5.5% of licorice, 0.9%-1.1% of peppermint, and 58%-62% of anhydrous glucose.

[0008] In an optional embodiment, the preparation method of the Lonicera japonica branch and leaf extract includes: collecting Lonicera japonica branches and leaves, washing them clean, drying them below 60°C with a moisture content ≤10%, pulverizing them with a pulverizer, and passing them through a No. 5 sieve for later use; taking 100g of the pulverized Lonicera japonica branches and leaves, adding distilled water at a ratio of 1:6-10, soaking for 1-3 hours to obtain an infusion solution; connecting a volatile oil extractor and a reflux condenser, placing the infusion solution in the volatile oil extractor, heating it to boiling and maintaining a gentle boil for 4-5 hours, finally turning off the power and letting it stand and cool for 1-2 hours, collecting the volatile oil in a brown bottle, refrigerating it at 4°C for later use; adding ethanol to the distilled infusion solution to a water-ethanol mixture with an ethanol concentration of 20-40%, decocting, filtering the decoction, concentrating it, adding the collected volatile oil, and making up to 100ml to obtain the Lonicera japonica branch and leaf extract containing 1g of the original herb per milliliter.

[0009] In an optional embodiment, the decoction is performed three times: the first time for 2-3 hours, the second time for 1.5-2 hours, and the third time for 1-1.5 hours. In a second aspect, the present invention provides a method for preparing a traditional Chinese medicine composition for preventing and treating inflammatory diseases in animals, comprising mixing the raw materials as described in any of the foregoing embodiments evenly.

[0010] Thirdly, the present invention provides the use of the traditional Chinese medicine composition for preventing and treating inflammatory diseases in animals as described in any of the foregoing embodiments in the preparation of a medicament for treating or improving inflammatory diseases in livestock and poultry.

[0011] In an optional embodiment, the traditional Chinese medicine composition for preventing and treating inflammatory diseases in animals reduces the concentrations of COX-2, IL-1β, IL-6, iNOS, PGE2, and TNF-α in serum while increasing the concentration of IL-10 in serum to achieve anti-inflammatory effects.

[0012] In an optional implementation, the avian inflammatory disease includes porcine reproductive and respiratory syndrome (PRRS) or avian infectious bronchitis.

[0013] In an optional embodiment, when the avian inflammatory disease is porcine reproductive and respiratory syndrome (PRRS), the traditional Chinese medicine composition for preventing and treating animal inflammatory diseases is added to the daily diet for feeding, and the amount of the traditional Chinese medicine composition for preventing and treating animal inflammatory diseases added is 8-12g / head.

[0014] In an optional embodiment, when the avian inflammatory disease is avian infectious bronchitis, the traditional Chinese medicine composition for preventing and treating animal inflammatory diseases is added to drinking water for feeding, and the amount of the traditional Chinese medicine composition for preventing and treating animal inflammatory diseases added is 3-5% of the mass of drinking water.

[0015] The present invention has the following beneficial effects:

[0016] The traditional Chinese medicine composition for the prevention and treatment of inflammatory diseases in animals provided in this application is a compound of extracts from the branches and leaves of *Lonicera japonica* and *Dipsacus asper*, along with the addition of chrysanthemum, mulberry leaf, licorice, peppermint, and anhydrous glucose. The resulting composition exhibits good anti-inflammatory effects and can effectively alleviate inflammatory responses caused by LPS. It is particularly effective in treating and improving the clinically prevalent porcine reproductive and respiratory syndrome (PRRS) and infectious bronchitis in chickens. Feeding PRRS-positive pigs with the composition at a dose of 10g / head for one week can effectively reduce morbidity and mortality, and improve production performance. Adding the composition to drinking water at a ratio of 3%-5% to treat chickens with infectious bronchitis for one week can effectively reduce mortality and increase the cure rate. Detailed Implementation

[0017] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Where specific conditions are not specified in the embodiments, conventional conditions or conditions recommended by the manufacturer shall apply. Reagents or instruments whose manufacturers are not specified are all conventional products that can be purchased commercially.

[0018] Natural plants contain a variety of active ingredients, including vitamins, minerals, proteins, glycosides, flavonoids, volatile oils, alkaloids, and sterols, in addition to nutrients such as vitamins, minerals, and proteins. These ingredients exhibit a variety of activities, including antibacterial, antiviral, anti-inflammatory, and immune-enhancing effects. When various traditional Chinese medicines are appropriately combined, they can synergistically enhance their efficacy. This invention provides a traditional Chinese medicine composition for the prevention and treatment of inflammatory diseases in animals, characterized by good anti-inflammatory effects, no residue, and few side effects.

[0019] Specifically, the raw materials of this traditional Chinese medicine composition for preventing and treating inflammatory diseases in animals, by mass percentage, include 8%-12% extract of honeysuckle branches and leaves, 8%-12% extract of dipsacus root, 8%-12% chrysanthemum, 4%-6% mulberry leaf, 4%-6% licorice, 0.8%-1.2% peppermint, and 50.8%-67.2% anhydrous glucose.

[0020] Preferably, the raw materials, by mass percentage, include 9%-11% of honeysuckle twig and leaf extract, 9%-11% of Sichuan teasel root extract, 9%-11% of chrysanthemum, 4.5%-5.5% of mulberry leaf, 4.5%-5.5% of licorice, 0.9%-1.1% of peppermint, and 58%-62% of anhydrous glucose.

[0021] The preparation method of the extract of honeysuckle branches and leaves includes: collecting honeysuckle branches and leaves, cleaning them, drying them below 60℃ with a moisture content of ≤10%, pulverizing them with a pulverizer, and passing them through a No. 5 sieve for later use; taking 100g of the pulverized honeysuckle branches and leaves, adding distilled water at a ratio of 1:6-10, soaking for 1-3 hours to obtain an infusion; connecting a volatile oil extractor and a reflux condenser, placing the infusion in the volatile oil extractor, heating it to boiling and maintaining a gentle boil for 4-5 hours, finally turning off the power and letting it stand and cool for 1-2 hours, collecting the volatile oil in a brown bottle, refrigerating it at 4℃ for later use; adding ethanol to the distilled infusion to a water-ethanol mixture with an ethanol concentration of 20-40%, decocting, filtering the decoction, concentrating it, adding the collected volatile oil, and making up to 100ml to obtain the honeysuckle branch and leaf extract containing 1g of the original drug per milliliter.

[0022] In this application, the alcohol extract and volatile oil are combined to form the honeysuckle branch and leaf extract. Compared with conventional alcohol extraction or water extraction, the specific extraction method in this application extracts the effective components more fully and has better effects.

[0023] It is worth noting that this application seeks protection for an extract of the branches and leaves of *Lonicera japonica*, not an extract of the flowers. Honeysuckle is a commonly used traditional Chinese medicine with effects of clearing heat and detoxifying, and dispersing wind-heat. The branches and leaves, byproducts of honeysuckle, have a high yield but have long been considered non-medicinal parts and thus unused. This application obtains an extract of *Lonicera japonica* branches and leaves through a specific preparation method, which can achieve better anti-inflammatory effects.

[0024] The decoction is prepared in three stages: the first stage lasts 2-3 hours, the second stage lasts 1.5-2 hours, and the third stage lasts 1-1.5 hours.

[0025] The present invention also provides a method for preparing a traditional Chinese medicine composition for preventing and treating inflammatory diseases in animals, which includes mixing the above-mentioned raw materials evenly.

[0026] The traditional Chinese medicine composition for preventing and treating inflammatory diseases in animals provided by this invention can be widely used in the preparation of drugs for treating or improving inflammatory diseases in livestock and poultry.

[0027] Specifically, the traditional Chinese medicine composition for preventing and treating inflammatory diseases in animals reduces the concentrations of COX-2, IL-1β, IL-6, iNOS, PGE2, and TNF-α in serum while increasing the concentration of IL-10 in serum to achieve anti-inflammatory effects.

[0028] The inflammatory diseases of birds mentioned in this disclosure include porcine reproductive and respiratory syndrome (PRRS) or avian infectious bronchitis.

[0029] When the inflammatory disease in poultry is porcine reproductive and respiratory syndrome (PRRS), a traditional Chinese medicine composition for preventing and treating inflammatory diseases in animals should be added to the daily diet. The dosage of the traditional Chinese medicine composition for preventing and treating inflammatory diseases in animals is 8-12g / head.

[0030] When the inflammatory disease in avian birds is infectious bronchitis, a traditional Chinese medicine composition for preventing and treating inflammatory diseases in animals is added to the drinking water for feeding. The amount of the traditional Chinese medicine composition for preventing and treating inflammatory diseases in animals is 3-5% of the mass of the drinking water.

[0031] The features and performance of the present invention will be further described in detail below with reference to embodiments.

[0032] Example 1

[0033] This embodiment provides a traditional Chinese medicine composition for preventing and treating inflammatory diseases in animals. The raw materials include: 10% extract of honeysuckle branches and leaves, 10% extract of Dipsacus asperoides, 10% chrysanthemum, 5% mulberry leaves, 5% licorice, 1% peppermint, and 59% anhydrous glucose.

[0034] The preparation method of the extract from the branches and leaves of Lonicera japonica includes:

[0035] Collect honeysuckle branches and leaves, clean them, dry them below 60℃ with a moisture content ≤10%, pulverize them using a pulverizer, and pass them through a No. 5 sieve for later use. Take 100g of the pulverized honeysuckle branches and leaves, add distilled water at a ratio of 1:8, and soak for 2 hours to obtain an infusion. Connect a volatile oil extractor and a reflux condenser, place the infusion in the volatile oil extractor, heat to boiling, and maintain a gentle boil for 4 hours. Finally, turn off the power and let it stand and cool for 1 hour. Collect the volatile oil in a brown bottle and store it at 4℃ for later use. Add ethanol to the distilled infusion to make a water-ethanol mixture with an ethanol concentration of 20-40%, and decoct three times: the first time for 2 hours, the second time for 1.5 hours, and the third time for 1 hour. Filter the decoction, combine and concentrate it, add the collected volatile oil, and make up to 100ml to obtain the honeysuckle branch and leaf extract containing 1g of raw drug per milliliter.

[0036] The supplier of the extract of Sichuan teasel root was Guanghan Sanxingdui Biotechnology Co., Ltd.

[0037] The preparation method of the traditional Chinese medicine composition for preventing and treating inflammatory diseases in animals includes: mixing the above raw materials evenly.

[0038] Example 2

[0039] This embodiment provides a traditional Chinese medicine composition for preventing and treating inflammatory diseases in animals. The raw materials include: 8% extract of honeysuckle branches and leaves, 8% extract of Dipsacus asper, 8% chrysanthemum, 4% mulberry leaves, 4% licorice, 0.8% peppermint, and 67.2% anhydrous glucose.

[0040] Example 3

[0041] This embodiment provides a traditional Chinese medicine composition for preventing and treating inflammatory diseases in animals. The raw materials include: 12% extract of honeysuckle branches and leaves, 12% extract of Dipsacus asperoides, 12% chrysanthemum, 6% mulberry leaves, 6% licorice, 1.2% peppermint, and 50.8% anhydrous glucose.

[0042] Comparative Example 1

[0043] This comparative example provides a traditional Chinese medicine composition that omits the extract of honeysuckle branches and leaves from Example 1 and supplements it with anhydrous glucose.

[0044] Specifically, its raw materials include 10% Dipsacus asper extract, 10% chrysanthemum, 5% mulberry leaf, 5% licorice, 1% peppermint, and 69% anhydrous glucose.

[0045] Comparative Example 2

[0046] This comparative example provides a traditional Chinese medicine composition that omits the extract of Dipsacus asperoides in Example 1 and makes up the difference with anhydrous glucose.

[0047] Specifically, its raw materials include 10% honeysuckle twig and leaf extract, 10% chrysanthemum, 5% mulberry leaf, 5% licorice, 1% peppermint, and 69% anhydrous glucose.

[0048] Comparative Example 3

[0049] This comparative example provides a traditional Chinese medicine composition that omits the extracts of honeysuckle branches and leaves and the extract of dipsacus root from Example 1, and makes up the difference with anhydrous glucose.

[0050] Specifically, its ingredients include 10% chrysanthemum, 5% mulberry leaves, 5% licorice, 1% peppermint, and 79% anhydrous glucose.

[0051] Comparative Example 4

[0052] This comparative example is basically the same as Example 1, except that the preparation method of the Lonicera japonica branch and leaf extract in this comparative example is alcohol extraction. Specifically: collect Lonicera japonica branches and leaves, clean them, dry them below 60°C with a moisture content of ≤10%, pulverize them with a pulverizer, and pass them through a No. 5 sieve for later use; take 100g of the pulverized Lonicera japonica branches and leaves, add 70% ethanol solution at a material-to-liquid ratio of 14:1, extract twice, each time for 1.5h, to prepare the extract, filter, recover the ethanol from the filtrate, concentrate to an appropriate amount, and vacuum dry into powder, which is the Lonicera japonica branch and leaf ethanol extract.

[0053] Comparative Example 5

[0054] This comparative example is basically the same as Example 1, except that the preparation method of the Lonicera japonica branch and leaf extract in this comparative example is water extraction. Specifically: collect Lonicera japonica branches and leaves, clean them, dry them below 60°C with a moisture content of ≤10%, pulverize them with a pulverizer, and pass them through a No. 5 sieve for later use; take 100g of the pulverized Lonicera japonica branches and leaves, add pure water at a material-to-liquid ratio of 14:1, extract twice, each extraction for 1.5h, to prepare the extract, filter, recover ethanol from the filtrate, concentrate to an appropriate amount, and vacuum dry into powder, which is the Lonicera japonica branch and leaf water extract.

[0055] Experimental Example 1: Investigating the anti-inflammatory properties of the composition.

[0056] (1) Grouping of animals

[0057] After 10 days of acclimatization feeding, SPF-grade BALB / c mice were randomly divided into 9 groups according to the principle of random grouping (animals were ear-tagged, weighed, and grouped by numbers randomly generated by SPSS software after being sorted by weight): composition experimental group, composition control group 1, composition control group 2, composition control group 3, composition control group 4, composition control group 5, LPS control group, dexamethasone group, and negative control group, with 6 mice in each group.

[0058] (2) Drug intervention

[0059] Each group was administered oral medication via gavage for 7 consecutive days, once daily, with a dosage of 0.2 g / animal. The LPS group, dexamethasone group, and negative control group received an equal volume of physiological saline. The dexamethasone group received an intramuscular injection of 10 mg / kg at 9:00 AM on days 6 and 7. Specific procedures for each group are as follows:

[0060] Table 1. Statistical Table of Trial Groups and Intervention Procedures

[0061]

[0062]

[0063] (3) Model building

[0064] Inflammation: Animals were given drug intervention for 7 consecutive days. One hour after intervention on the 7th day, each group was injected intraperitoneally with LPS (10mg / kg) and simultaneously injected subcutaneously with 0.1mL of 1% carrageenan solution in the left foot to induce inflammation. Three hours later, xylene was used to induce inflammation. 10μL of xylene was evenly applied to both the front and back of the left auricle (20μL / animal).

[0065] (4) Evaluation indicators

[0066] 4.1 Observation of general clinical features

[0067] After the experiment began, the general condition of each group of mice was observed and recorded at a fixed time each week: mental state, freedom of movement, hair luster, fecal condition and urination. The observation was conducted twice a week.

[0068] 4.2 Ear swelling degree

[0069] Mice were euthanized and both ears were cut off along the auricular baseline. Ear flaps were punched out from the same location on both ears using an ear swelling puncher, and weighed separately. The difference in weight between the left and right ear flaps was used to represent the degree of swelling. Calculation formula: Inhibition rate = [(average swelling degree of blank control group - average swelling degree of treatment group) / average swelling degree of blank control group] × 100%.

[0070] 4.3 Foot swelling

[0071] Both hind feet were cut off at the ankle joint, and the thickness of the inflamed foot was measured using calipers. The difference in volume between the inflamed and inflamed feet was used to represent the degree of swelling.

[0072] 4.4 Serological Inflammation-Related Indicators Detection and Analysis

[0073] Blood was collected from mice in each group to separate serum, and the changes in the levels of COX-2, IL-1β, IL-6, IL-10, iNOS, and TNF-α in the serum were detected by ELISA.

[0074] (5) Experimental Results

[0075] 5.1 General Clinical Observation Results

[0076] The mice in all groups had stable vital signs and good mental state, with no lethargy, coughing, wheezing, groaning, drooling, or abnormal secretions, and no unexpected deaths occurred. During exercise, the head, neck, waist, back, and limbs were in normal condition, and no lameness, paralysis, arched back, staggering gait, or falling behind the group were observed.

[0077] On the seventh day, mice injected intraperitoneally with LPS exhibited symptoms such as lethargy, sluggishness, and unformed feces.

[0078] 5.2 Only the ear swelling inhibition rate and foot swelling inhibition rate were measured in the four groups: negative control group, LPS control group, dexamethasone group, and combination experimental group. The results of the ear swelling inhibition rate and foot swelling inhibition rate are shown in Table 2.

[0079] Table 2. Statistical table of inhibition rates for ear swelling and foot swelling

[0080] Group Ear swelling inhibition rate Foot swelling inhibition rate negative control group 0 0 LPS control group 2.1％ 2.5％ Dexamethasone group 21.6％ 41.9％ Composition Experimental Group 15.0％ 17.3％

[0081] The results showed that the experimental group of the composition had a good inhibitory effect on xylene-induced ear swelling and carrageenan-induced paw swelling in mice.

[0082] 5.3. Please refer to Table 3 for the results of various inflammatory markers in serum:

[0083] Table 3. Changes in serum COX-2 concentration in mice of different groups

[0084]

[0085]

[0086] Note: Compared with the negative control group, the LPS control group... * P<0.05, ** P<0.01; compared with the LPS control group, the remaining groups... # P<0.05, ## P < 0.01 (the same applies below);

[0087] Table 3 shows that, compared with the negative control group, the serum COX-2 concentration in mice in the LPS control group was significantly increased (P<0.01). Compared with the LPS control group, the serum COX-2 concentration in mice in the dexamethasone group and the combination group was significantly decreased (P<0.01); the serum COX-2 concentration in the five combination control groups was significantly decreased (P<0.05), while the other combination control groups showed no significant difference.

[0088] Table 4. Changes in serum IL-1β concentration in mice of different groups

[0089]

[0090]

[0091] Table 4 shows that, compared with the negative control group, the serum IL-1β concentration in mice in the LPS control group was significantly increased (P<0.01). Compared with the LPS control group, the serum IL-1β concentration in mice in the composition experimental group was significantly decreased (P<0.01).

[0092] Table 5. Changes in serum IL-6 concentration in mice of different groups.

[0093] Group Concentration (pg / mL) negative control group 19.957±2.872 LPS control group <![CDATA[30.609±6.160 ** ]]> Dexamethasone group <![CDATA[23.038±2.717 # ]]> Composition Experimental Group <![CDATA[22.529±2.883 ## ]]> Composition Control Group 1 23.462±3.910# Composition control group 2 24.300±3.692 Composition control group 3 26.047±3.384 Composition control group 4 25.421±2.492 Composition control group 5 26.210±3.149

[0094] Table 5 shows that, compared with the negative control group, the serum IL-6 concentration in mice in the LPS control group was significantly increased (P<0.01). Compared with the LPS control group, the serum IL-6 concentration in mice in the combined experimental group was significantly decreased (P<0.01); the serum IL-6 concentration in mice in the dexamethasone group was also significantly decreased (P<0.05).

[0095] Table 6. Changes in serum IL-10 concentration in mice of different groups.

[0096]

[0097]

[0098] Table 6 shows the statistical results. Compared with the negative control group, the serum IL-10 concentration in mice in the LPS control group was significantly lower (P<0.01). Compared with the LPS control group, the serum IL-10 concentration in mice in the composition experimental group was significantly higher (P<0.01); the serum IL-10 concentration in mice in the dexamethasone group was also significantly higher (P<0.05).

[0099] Table 7. Changes in serum TNF-α concentration in mice of different groups.

[0100] Group Concentration (pg / mL) negative control group 355.168±16.668 LPS control group <![CDATA[428.307±23.304 ** ]]> Dexamethasone group <![CDATA[387.890±19.980 # ]]> Composition Experimental Group <![CDATA[370.080±24.304 ## ]]> Composition Control Group 1 396.856±14.925 Composition control group 2 380.080±24.304# Composition control group 3 397.737±31.228 Composition control group 4 390.120±28.921 Composition control group 5 395.379±31.442

[0101] Table 7 shows that, compared with the negative control group, the serum TNF-α concentration in mice in the LPS control group was significantly increased (P<0.01). Compared with the LPS control group, the serum TNF-α concentration in mice in the combined experimental group was significantly decreased (P<0.01); the serum TNF-α concentration in mice in the dexamethasone group was also significantly decreased (P<0.05).

[0102] Serological test results showed that mice administered the composition by gavage could alleviate the inflammatory response induced by LPS, which was manifested as a reduction in visible inflammatory symptoms and a decrease in the values ​​of multiple hematological inflammatory markers, with significant differences compared with the control group.

[0103] Experimental Example 2: Investigating the effect of the composition on porcine reproductive and respiratory syndrome (PRRS) positive pig farms

[0104] Three hundred and fifteen piglets weighing approximately 20 kg from a porcine reproductive and respiratory syndrome (PRRS) positive farm were randomly divided into seven groups: a blank control group, a composition experimental group, composition control group 1, composition control group 2, composition control group 3, composition control group 4, and composition control group 5. During feeding, the composition experimental group was administered the composition provided in Example 1 at a dosage of 10 g / head for seven consecutive days; composition control group 1 was administered the composition provided in Comparative Example 1 at a dosage of 10 g / head for seven consecutive days; composition control group 2 was administered the composition provided in Comparative Example 2 at a dosage of 10 g / head for seven consecutive days; composition control group 3 was administered the composition provided in Comparative Example 3 at a dosage of 10 g / head for seven consecutive days; composition control group 4 was administered the composition provided in Comparative Example 4 at a dosage of 10 g / head for seven consecutive days; composition control group 5 was administered the composition provided in Comparative Example 4 at a dosage of 10 g / head for seven consecutive days; and the blank control group was fed normally. All piglets were managed under the same conditions, and the observation period was one month.

[0105] Table 8 Statistical Table of Experimental Results

[0106]

[0107] Experimental results: During the experimental period, the growth rate and mental state of the pigs in the experimental group were better than those in the control group, and no pigs fell ill. In the control groups 1-5, 1-2 pigs fell ill, but no pigs died. In the blank control group, 6 pigs fell ill, showing symptoms of fever, high blood pressure, panting, and conjunctivitis. After symptomatic treatment, 4 pigs recovered, and 2 pigs died.

[0108] Conclusion: Feeding the composition continuously for one week can reduce the incidence of porcine reproductive and respiratory syndrome (PRRS).

[0109] Experiment Example 3: Investigating the therapeutic effect of the composition on infectious bronchitis in chickens.

[0110] (1) Experimental animals:

[0111] Naturally diseased chicken flocks were collected clinically, and positive flocks were included in the experimental animals based on a comprehensive assessment of clinical symptoms and laboratory PCR test results.

[0112] (2) Experimental grouping:

[0113] 1900 sick chickens were randomly divided into seven groups: a composition experimental group, composition control groups 1-5, and a blank control group. The medication was administered via drinking water. Each of the six groups (composition experimental group and composition control groups 1-5) contained 300 chickens. The composition experimental group received the composition provided in Example 1 in the specified proportions. The blank control group (100 chickens) received no drug treatment. Composition control group 1 received the composition provided in Example 1 at a 5% dosage; Composition control group 2 received the composition provided in Example 2 at a 5% dosage; Composition control group 3 received the composition provided in Example 3 at a 5% dosage; Composition control group 4 received the composition provided in Example 4 at a 5% dosage; and Composition control group 5 received the composition provided in Example 5 at a 5% dosage.

[0114] (3) Evaluation of therapeutic effect:

[0115] Daily observation and recording of the flock's mental and respiratory status, feed intake, and mortality were conducted. The effectiveness of the treatment was assessed and statistically analyzed one week after treatment according to the following criteria:

[0116] Cure: The clinical symptoms of the chickens disappeared, the coughing stopped, and their feces, mental state, and feed intake returned to normal.

[0117] Improvement: The patient's mental state, appetite, and feces have basically returned to normal, and clinical symptoms such as coughing have been significantly reduced. With continued strengthening of feeding management and disease prevention and control, growth performance can return to normal.

[0118] Overall effectiveness rate = cure rate + improvement rate.

[0119] Ineffective: Symptoms do not improve or even worsen or cause death; or the patient does not die within a week but is weak and has slow growth.

[0120] (4) Test Results

[0121] Table 9 Treatment effects of each experimental group

[0122] Group cure rate Improvement rate Overall efficiency mortality rate Composition Experimental Group 69.30％ 20.70％ 90.00％ 10.00％ Blank control group 14.70％ 10.70％ 25.30％ 36.00％ Composition Control Group 1 61.20％ 20.10％ 81.30％ 12.67％ Composition control group 2 62.80％ 17.40％ 80.20％ 15.00％ Composition control group 3 63.30％ 16.40％ 79.70％ 11.33％ Composition control group 4 59.40％ 21.10％ 80.50％ 18.67％ Composition control group 5 61.70％ 18.80％ 81％ 19.33％

[0123] The results showed that the cure rate and total effective rate of the experimental group and the control group were significantly higher than those of the blank control group, and the mortality rate was significantly lower than that of the untreated blank control group; the cure rate and total effective rate of the experimental group were significantly higher than those of the control group.

[0124] Conclusion: Adding the composition at a dosage of 5% to drinking water for the prevention and treatment of infectious bronchitis in chickens for one week can effectively improve the cure rate and reduce the morbidity rate.

[0125] In summary, the traditional Chinese medicine composition for preventing and treating inflammatory diseases in animals provided in this application, which combines extracts of honeysuckle branches and leaves with extracts of Dipsacus asperoides, and also includes chrysanthemum, mulberry leaves, licorice, peppermint, and anhydrous glucose, exhibits good anti-inflammatory effects and can effectively alleviate inflammatory responses caused by LPS. It is particularly effective in treating and improving common clinical diseases such as porcine reproductive and respiratory syndrome (PRRS) and infectious bronchitis in chickens. Feeding PRRS-positive pigs with the composition at 10g / head for one week can effectively reduce morbidity and mortality, and improve production performance. Adding the composition at a 5% ratio to drinking water to treat chickens with infectious bronchitis for one week can effectively reduce mortality and increase cure rates.

[0126] The above description is merely a preferred embodiment of the present invention and is not intended to limit the invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A traditional Chinese medicine composition for preventing and treating inflammatory diseases in animals, characterized in that, It is made from the following raw materials by weight percentage: 8%-12% honeysuckle twig and leaf extract, 8%-12% Sichuan teasel root extract, 8%-12% chrysanthemum, 4%-6% mulberry leaf, 4%-6% licorice, 0.8%-1.2% peppermint, and 50.8%-67.2% anhydrous glucose; The preparation method of the Lonicera japonica branch and leaf extract includes: collecting Lonicera japonica branches and leaves, cleaning them, drying them below 60℃ with a moisture content ≤10%, pulverizing them with a pulverizer, and passing them through a No. 5 sieve for later use; taking 100g of the pulverized Lonicera japonica branches and leaves, adding distilled water at a ratio of 1:6-10, soaking for 1-3 hours to obtain an infusion; connecting a volatile oil extractor and a reflux condenser, placing the infusion in the volatile oil extractor, heating it to boiling and maintaining a gentle boil for 4-5 hours, finally turning off the power and letting it stand and cool for 1-2 hours, collecting the volatile oil in a brown bottle, refrigerating it at 4℃ for later use; adding ethanol to the distilled infusion to a water-ethanol mixture with an ethanol concentration of 20-40%, decocting, filtering the decoction, concentrating it, adding the collected volatile oil, and making up to 100ml to obtain the Lonicera japonica branch and leaf extract containing 1g of the original herb per milliliter.

2. The Chinese medicine composition for preventing and treating inflammatory diseases of animals according to claim 1, characterized in that, It is made from the following raw materials by mass percentage: 9%-11% extract of honeysuckle branches and leaves, 9%-11% extract of Sichuan teasel root, 9%-11% chrysanthemum, 4.5%-5.5% mulberry leaf, 4.5%-5.5% licorice, 0.9%-1.1% peppermint, and 58%-62% anhydrous glucose.

3. The traditional Chinese medicine composition for preventing and treating inflammatory diseases in animals according to claim 1, characterized in that, The decoction is prepared in three stages: the first stage lasts 2-3 hours, the second stage lasts 1.5-2 hours, and the third stage lasts 1-1.5 hours.

4. A method for preparing a traditional Chinese medicine composition for preventing and treating inflammatory diseases in animals, characterized in that, It includes mixing the raw materials as described in any one of claims 1-3 evenly.

5. The use of the traditional Chinese medicine composition for preventing and treating inflammatory diseases in animals as described in any one of claims 1-3 in the preparation of anti-inflammatory drugs.

6. The application according to claim 5, characterized in that, The traditional Chinese medicine composition for preventing and treating inflammatory diseases in animals reduces the concentrations of COX-2, IL-1β, IL-6 and TNF-α in serum while increasing the concentration of IL-10 in serum to achieve anti-inflammatory effects.

7. The use of the traditional Chinese medicine composition for preventing and treating inflammatory diseases in animals as described in any one of claims 1-3 in the preparation of a medicament for treating or improving porcine reproductive and respiratory syndrome (PRRS) or infectious bronchitis in chickens.

Images