Multi-effect peach resin extract composition and application thereof in cosmetics

Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to a multi-effect peach resin extract composition and application thereof in cosmetics. The extract composition provided by the invention comprises a peach resin extract, an abelmoschus esculentus fruit extract and a portulaca oleracea extract, and the peach resin extract, the abelmoschus esculentus fruit extract and the portulaca oleracea extract are compounded according to the mass ratio of (1-20): (1-20): (1-50), so that the extract composition has a synergistic interaction effect and can synergistically improve the instant moisturizing and long-acting moisturizing effects; and the extract is natural, green, high in safety, mild and non-irritating, has relatively good smearing absorption performance, and can effectively improve the skin feeling of a skin care product, improve the smearing ductility of the product and the softness of the dried skin and reduce the sticky feeling of the product. The skin sensitivity and discomfort of adults can be effectively relieved, and the problems of infant eczema, dry and red skin and the like can also be relieved.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and particularly relates to a multi-effect peach resin extract composition and its application in cosmetics. Background Art

[0002] Sensitive skin is mostly caused by damaged cutin barrier and increased transdermal water loss rate, resulting in overreaction of skin nerve receptors to external stimuli, and various skin discomfort symptoms such as dryness, tightness, burning, itching, etc. Secondly, it will also activate the cellular immune response, leading to the release of inflammatory factors and mediators, infiltration of inflammatory cells, and skin congestion and swelling.

[0003] Currently, the solutions of the prior art for sensitive skin mostly achieve a soothing effect through different skin pathways and targets. For example, the prior art CN117462437A discloses a mussel protein composition that acts through multi-pathway synergy, has good skin barrier repair, inhibits inflammatory response, and alleviates subcutaneous nerve overfeedback, and is suitable for sensitive skin. However, the mechanisms of these products for alleviating sensitive skin in people mainly rely on repairing the epidermal barrier or reducing the susceptibility of skin terminal sensory neurons. Although they can relieve the sensitivity mediated by irritant reactions, they ignore the role of skin moisturization and cutin barrier repair in alleviating skin sensitivity. Therefore, there is an urgent need in this field to develop a product that can effectively relieve the discomfort of sensitive skin and exert excellent moisturizing, repairing, and soothing effects to meet the needs of the increasing number of people with sensitive skin. Summary of the Invention

[0004] The purpose of the present invention is to provide a multi-effect peach resin extract composition and its application in cosmetics, which can exert excellent moisturizing, repairing, and soothing effects and improve the skin feel experience of skin care products.

[0005] The present invention provides an extract composition, comprising peach resin extract, okra fruit extract, and purslane extract;

[0006] The mass ratio of peach resin extract, okra fruit extract, and purslane extract in the extract composition is (1 - 20):(1 - 20):(1 - 50).

[0007] Preferably, the mass ratio of peach resin extract, okra fruit extract, and purslane extract in the extract composition is 1:1:(1 - 10).

[0008] Preferably, the mass ratio of peach resin extract, okra fruit extract, and purslane extract in the extract composition is 1:1:2.

[0009] The present invention also provides the application of the extract composition described in the above technical solution in the preparation of cosmetics.

[0010] Preferably, the cosmetic is a cosmetic having one or more effects of moisturizing, soothing and repairing.

[0011] Preferably, the mass percentage content of the extract composition in the cosmetic ≥ 0.1%.

[0012] Preferably, the mass percentage content of the extract composition in the cosmetic is 0.1% - 5%.

[0013] Preferably, the dosage form of the cosmetic includes one or more of cream, lotion, essence, toner, gel, mask, shampoo, hair conditioner, facial cleanser and body wash.

[0014] Beneficial effects:

[0015] The present invention provides an extract composition, comprising peach resin extract, okra fruit extract and purslane extract; the mass ratio of peach resin extract, okra fruit extract and purslane extract in the extract composition is (1 - 20):(1 - 20):(1 - 50). The peach resin extract not only has excellent water replenishing and moisturizing effects, but also can promote wound self-healing, repair the active monomers of damaged skin, and is easy to penetrate through the skin. Its skin feel is smooth, moist and non-greasy; the okra fruit extract has good moisturizing and repairing effects, and its skin feel is smooth and non-greasy; the purslane extract has antibacterial and anti-inflammatory effects, can reduce the secretion of inflammatory factors, has a significant soothing and antipruritic effect, and can also promote wound healing. The present invention combines peach resin extract, okra fruit extract and purslane extract according to a certain mass ratio, which has a synergistic effect, can synergistically improve the instant water replenishing and long-term moisturizing effects, as well as the cutin barrier repair and soothing effects. Moreover, the extract is natural, green, high in safety, mild and non-irritating, has good spreading and absorption properties, can effectively improve the skin feel of skin care products, enhance the spreading property of the product during application and the softness of the skin after drying, and reduce the greasiness of the product. It can effectively relieve the skin sensitivity discomfort of adults, and can also be used to relieve problems such as infantile eczema and dry and red skin. Description of the drawings

[0016] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required to be used in the embodiments.

[0017] Figure 1 For the influence of different test samples in Example 1 on cell migration rate;

[0018] Figure 2 For the influence of different test samples in Example 2 on the content of cell inflammatory factors;

[0019] Figure 3For Example 3, the effects of different test samples on the dry cell mortality rate;

[0020] Figure 4 For Example 8, the effects of different creams on the improvement rate of skin water content;

[0021] Figure 5 For Example 8, the effects of different creams on the improvement rate of the percutaneous water loss rate. Detailed implementation manners

[0022] The present invention provides an extract composition, comprising a peach resin extract, an okra fruit extract, and a purslane extract;

[0023] In the extract composition, the mass ratio of the peach resin extract, the okra fruit extract, and the purslane extract is (1 - 20):(1 - 20):(1 - 50).

[0024] As an implementation manner, in the extract composition of the present invention, the mass ratio of the peach resin extract, the okra fruit extract, and the purslane extract is 1:1:(1 - 10); as another implementation manner, in the extract composition of the present invention, the mass ratio of the peach resin extract, the okra fruit extract, and the purslane extract is 1:1:2.

[0025] As an implementation manner, the peach resin extract of the present invention is a peach resin extract of a peach tree planted in the Qinghai-Tibet Plateau region. The ultraviolet radiation in the Qinghai-Tibet Plateau region is strong, the temperature difference between day and night is large, and the growth environment is harsh. The peach gum secreted by the peach trees planted in the Qinghai-Tibet Plateau region has a higher content of active ingredients than the peach gum in conventional regions. The present invention has no strict requirements on the source of the peach resin extract, and it can be purchased conventionally. The peach resin extract used in the examples of the present invention is a water extract of peach resin. The present invention has no strict requirements on the preparation method of the peach resin extract, and it can be purchased conventionally or extracted and prepared by itself in a conventional manner. The peach resin extract used in the present invention not only has excellent water replenishing and moisturizing effects, but also can promote wound self-healing, repair damaged skin active monomers, and is easy to penetrate through the skin, and its skin feeling is smooth, moist and not sticky.

[0026] As an implementation manner, the okra fruit extract of the present invention is a water extract of okra fruit. The present invention has no strict requirements on the source of the okra fruit extract, and it can be purchased conventionally or extracted and prepared by itself in a conventional manner. The okra fruit extract used in the present invention has good moisturizing and repairing effects, and its skin feeling is smooth and not sticky.

[0027] As an implementation mode, the purslane extract in the present invention is a water extract of purslane. The present invention has no strict requirements on the source of the purslane extract, which can be purchased conventionally or prepared by self-extraction in a conventional manner. The present invention utilizes the purslane extract to achieve a significant soothing and antipruritic effect, and at the same time can promote wound healing.

[0028] The present invention compounded the peach resin extract, the okra fruit extract and the purslane extract according to a certain mass ratio, which has a synergistic effect, and can synergistically improve the instant water replenishment and long-term moisturizing effects, as well as the horny barrier repair and soothing effects. Moreover, the obtained extract composition has good spreading and absorption properties, can effectively improve the feel of skin care products, enhance the extensibility of product spreading and the softness of the skin after drying, and reduce the greasiness of the product. The components of the extract composition of the present invention are natural and green, and the obtained extract composition has high safety, is mild and non-irritating, and can not only effectively relieve the skin sensitivity discomfort of adults, but also be used to relieve problems such as infantile eczema and dry and flaky skin.

[0029] In view of the advantages of the extract composition provided by the present invention, the application of the extract composition described in the above technical solution in the preparation of cosmetics also belongs to the protection scope of the present invention.

[0030] As an implementation mode, the cosmetics in the present invention are cosmetics having one or more of the functions of moisturizing, soothing and repairing; as another implementation mode, the cosmetics in the present invention are cosmetics having the functions of moisturizing, soothing and repairing.

[0031] As an implementation mode, the mass percentage content of the extract composition in the cosmetics of the present invention is ≥0.1%; as another implementation mode, the mass percentage content of the extract composition in the cosmetics of the present invention is 0.1% - 5%; as another implementation mode, the mass percentage content of the extract composition in the cosmetics of the present invention is 0.2% - 2%; as another implementation mode, the mass percentage content of the extract composition in the cosmetics of the present invention is 0.4% - 1%.

[0032] As an implementation mode, the dosage forms of the cosmetics of the present invention include one or more of creams, lotions, essences, toners, gels, masks, shampoos, conditioners, facial cleansers and body washes.

[0033] The percentage contents involved in the following examples are all mass percentage concentrations.

[0034] Example 1

[0035] Cell migration experiment

[0036] 1. Preparation of test samples

[0037] Sample 1: Add peach resin extract to DMEM culture medium to a final concentration of 0.4%;

[0038] Sample 2: Add okra fruit extract to DMEM culture medium to a final concentration of 0.4%;

[0039] Sample 3: Add purslane extract to DMEM culture medium to a final concentration of 0.4%;

[0040] Sample 4: Add peach resin extract, okra fruit extract and purslane extract to DMEM culture medium, so that the final concentrations of peach resin extract, okra fruit extract and purslane extract are 0.1%, 0.1% and 0.2% in sequence.

[0041] 2. Collect HaCaT cells (human immortalized keratinocytes) in the logarithmic growth phase, adjust the cell density to 5×10 5 cells / mL, inoculate into a 6-well plate, and culture overnight. Use a micropipette tip to scratch in the central area of cell growth, remove the central part, rinse the scratched part with PBS, randomly divide into a blank control group and a dosing group. The blank control group is added with fresh serum-free DMEM medium, and the dosing group is added with the same volume of the test sample. Incubate in an incubator for 24 h, then observe the scratch width with a microscope and take pictures, and calculate the cell migration rate according to the cell migration distance. The results are shown in Table 1 and Figure 1 .

[0042] Table 1 Cell migration rates of different treatment groups

[0043]

[0044] Note: Compared with the blank control group, * P<0.05, compared with Sample 3, # P<0.05.

[0045] According to Table 1 and Figure 1 it can be seen that compared with the blank control group, the individual peach resin extract (Sample 1), okra fruit extract (Sample 2), and purslane extract (Sample 3) can all improve the cell migration ability to a certain extent, and there is a significant difference between Sample 1 and Sample 3; further, compared with Sample 3, the cell migration rate of the compound sample of peach resin extract, okra fruit extract and purslane extract (Sample 4) is further improved, and there is a significant difference. The extract composition of the present invention has an excellent effect of repairing damaged cells, and peach resin extract, okra fruit extract and purslane extract have a synergistic effect on the cell repair effect.

[0046] Example 2

[0047] Cell anti-inflammatory experiment

[0048] 1. Preparation of test samples

[0049] Sample 1: Add peach resin extract to DMEM culture medium to a final concentration of 0.4%;

[0050] Sample 2: Add okra fruit extract to DMEM culture medium to a final concentration of 0.4%;

[0051] Sample 3: Add purslane extract to DMEM culture medium to a final concentration of 0.4%;

[0052] Sample 4: Add peach resin extract, okra fruit extract and purslane extract to DMEM culture medium, and make the final concentrations of peach resin extract, okra fruit extract and purslane extract be 0.1%, 0.1% and 0.2% in turn.

[0053] 2. Seed RAW 264.7 cells into a 24-well plate at a density of 5×10 5 cells per well, and culture them in a 37°C, 5% CO2 cell culture incubator for 24 h. After the culture is completed, aspirate the medium in the cell plate, and randomly divide it into a blank control group, a model group and a drug administration group. Except for the blank control group, add 1 mg / mL of LPS to each well to establish a cell inflammation model. Add the test samples to the drug administration group respectively, and add an equal volume of serum-free medium to the blank control group, and continue to cultivate for 48 h. After 48 h, collect the cell supernatant, and detect the inflammatory factors TNF-α, IL-6 and IL-1β according to the instructions of the ELISA kit, and calculate the contents of TNF-α, IL-6 and IL-1β in the cell supernatant. The results are shown in Table 2 and Figure 2 as follows.

[0054] Table 2 Determination results of inflammatory factor contents in different treatment groups

[0055]

[0056] Note: Compared with the model group, * P < 0.05; compared with Sample 3, # P < 0.05.

[0057] The total contents of active ingredients in Samples 1-4 are the same. According to Table 2 and Figure 2It can be seen that, compared with the model group, the individual extracts of peach resin (Sample 1), Abelmoschus esculentus fruit (Sample 2), and Portulaca oleracea (Sample 3) can inhibit the secretion of TNF-α, IL-6, and IL-1β by RAW 264.7 cells to a certain extent, among which the extract of Portulaca oleracea (Sample 3) shows a significant difference. Further, compared with the extract of Portulaca oleracea (Sample 3), the compound sample of peach resin extract, Abelmoschus esculentus fruit extract, and Portulaca oleracea extract (Sample 4) further improves the inhibitory effect on the secretion of TNF-α, IL-6, and IL-1β by RAW 264.7 cells, showing a significant difference and significantly inhibiting the secretion of inflammatory factors. The extract composition of the present invention has a good soothing effect, and the peach resin extract, Abelmoschus esculentus fruit extract, and Portulaca oleracea extract have a synergistic effect on the soothing efficacy.

[0058] Example 3

[0059] Cell drying experiment

[0060] 1. Preparation of test samples

[0061] Sample 1: Add peach resin extract to DMEM culture medium to a final concentration of 0.4%;

[0062] Sample 2: Add Abelmoschus esculentus fruit extract to DMEM culture medium to a final concentration of 0.4%;

[0063] Sample 3: Add Portulaca oleracea extract to DMEM culture medium to a final concentration of 0.4%;

[0064] Sample 4: Add peach resin extract and Abelmoschus esculentus fruit extract to DMEM culture medium, so that the final concentrations of peach resin extract and Abelmoschus esculentus fruit extract are 0.2% and 0.2% respectively;

[0065] Sample 5: Add peach resin extract and Abelmoschus esculentus fruit extract to DMEM culture medium, so that the final concentrations of peach resin extract and Abelmoschus esculentus fruit extract are 0.1% and 0.3% respectively;

[0066] Sample 6: Add peach resin extract and Abelmoschus esculentus fruit extract to DMEM culture medium, so that the final concentrations of peach resin extract and Abelmoschus esculentus fruit extract are 0.3% and 0.1% respectively;

[0067] Sample 7: Add diluted peach resin extract, Abelmoschus esculentus fruit extract, and Portulaca oleracea extract to DMEM culture medium, so that the final concentrations of peach resin extract, Abelmoschus esculentus fruit extract, and Portulaca oleracea extract are 0.1%, 0.1%, and 0.2% respectively;

[0068] Sample 8: Diluted peach resin extract, Abelmoschus esculentus fruit extract and Portulaca oleracea extract were added to DMEM culture medium, so that the final concentrations of peach resin extract, Abelmoschus esculentus fruit extract and Portulaca oleracea extract were 0.1%, 0.1% and 0.1% in sequence;

[0069] Sample 9: Diluted peach resin extract, Abelmoschus esculentus fruit extract and Portulaca oleracea extract were added to DMEM culture medium, so that the final concentrations of peach resin extract, Abelmoschus esculentus fruit extract and Portulaca oleracea extract were 0.1%, 0.1% and 0.5% in sequence;

[0070] Sample 10: Diluted peach resin extract, Abelmoschus esculentus fruit extract and Portulaca oleracea extract were added to DMEM culture medium, so that the final concentrations of peach resin extract, Abelmoschus esculentus fruit extract and Portulaca oleracea extract were 0.1%, 0.1% and 1% in sequence;

[0071] Sample 11: Diluted peach resin extract, Abelmoschus esculentus fruit extract and Portulaca oleracea extract were added to DMEM culture medium, so that the final concentrations of peach resin extract, Abelmoschus esculentus fruit extract and Portulaca oleracea extract were 0.2%, 0.2% and 0.5% in sequence.

[0072] 2. According to the existing technology (Chen Mo, et al. Study on the Moisturizing Performance of Dendrobium officinale Extracts [J]. Acta Universitatis Traditionis Medicalis Sinensis Pharmacologiaeque Shanghai, 2015, 29(6): 70 - 73.), a cell dry injury model was established, and the MTT method was used to detect the ability of cells to resist drying, prevent dehydration death and cell repair. The specific steps are as follows:

[0073] Collect cells in the logarithmic growth phase, adjust the density of HaCaT cells to 3×10 4 cells and inoculate them into a 96 - well plate, and culture them in a cell incubator at 37°C and 5% CO2 for 24 h. Randomly divide them into a blank control group, a model group and a drug - administration group. After the culture is completed, aspirate the culture medium in the cell plate. The blank control group and the model group each add 500 μL of serum - free medium, and the drug - administration group adds 500 μL of the test sample for incubation. After 24 h, the blank control group is not treated with anything. The model group and the drug - administration group are placed in a laminar flow hood and dried at a certain wind speed. Then, fresh culture medium is added to each group. After continuing to culture for 24 h, 10 μL of CCK - 8 solution is added to each well and incubated in the incubator for 2 h. Use an enzyme - linked immunosorbent assay reader to measure the OD value at 450 nm, and calculate the cell dry mortality rate (%) according to the following formula. The results are shown in Table 3 and Figure 3 as follows.

[0074] Cell dry mortality rate (%)=(1 - OD value of the detection group / OD value of the blank control group)×100%;

[0075] Table 3 Results of cell dry mortality rates in different treatment groups

[0076]

[0077] Note: Compared with the model group, * P < 0.05; compared with Sample 1, # P < 0.05; compared with Sample 4, & P < 0.05.

[0078] According to Table 3 and Figure 3 It can be seen that after drying treatment, the dry mortality rate of cells in the model group increased significantly, indicating that the cell dry injury model was successfully established. The total content of active ingredients in Samples 1-7 is the same. Compared with the dry mortality rate of the model group, the dry mortality rates of Samples 1-3 decreased, indicating that the extract of Prunus persica resin, the extract of Abelmoschus esculentus fruit, and the extract of Portulaca oleracea alone have a certain effect on resisting dry injury. Among them, the extracts of Prunus persica resin and Abelmoschus esculentus fruit have a better anti-dry injury effect than the extract of Portulaca oleracea; compared with Sample 1 (extract of Prunus persica resin), the dry mortality rate of Sample 4 (0.2% extract of Prunus persica resin and 0.2% extract of Abelmoschus esculentus fruit) decreased further, with a significant difference, indicating that when the extracts of Prunus persica resin and Abelmoschus esculentus fruit are compounded at a mass ratio of 1:1, the synergistic effect is optimal, and the anti-dry injury effect is better than that of a single component. Compared with Sample 4, the dry mortality rates of Samples 7-11 decreased further and significantly, indicating that further combination of the extracts of Prunus persica resin, Abelmoschus esculentus fruit and Portulaca oleracea can further improve the anti-dry and cell repair effects of the composition, with a synergistic effect. Among them, the dry mortality rate of Sample 7 (0.1% extract of Prunus persica resin, 0.1% extract of Abelmoschus esculentus fruit and 0.2% extract of Portulaca oleracea) decreased most significantly, indicating that when the extracts of Prunus persica resin, Abelmoschus esculentus fruit and Portulaca oleracea are compounded at a mass ratio of 1:1:2, the synergistic effect is optimal. The extract composition of the present invention has excellent moisturizing and cell repair capabilities and can improve the ability of cells to resist drying.

[0079] Example 4

[0080] An extract composition cream is prepared from the following raw materials by weight percentage: 0.5% extract of Prunus persica resin, 0.5% extract of Abelmoschus esculentus fruit, 1% extract of Portulaca oleracea, 8% caprylic / capric triglyceride, 5% coco-caprylate / caprate, 2% cetearyl alcohol, 2% steareth-2, 2% steareth-21, 5% propylene glycol, 0.15% carbomer, 0.15% triethanolamine, 0.5% phenoxyethanol and the balance of purified water.

[0081] The specific preparation method is as follows:

[0082] (1) heating caprylic capric triglyceride, coconut oil caprylate / caprate, cetearyl alcohol, steareth-2 and steareth-21 in a water bath at 75° C. to melt to obtain an oil phase;

[0083] (2) dissolving propylene glycol, carbomer and purified water in a 75° C. water bath to obtain an aqueous phase;

[0084] (3) adding the oil phase of step (2) dropwise to the water phase of step (1) and stirring and mixing, emulsifying at a rotation speed of 12000 rpm for 3 min, adding triethanolamine, continuing shearing for 2 min, cooling to 45° C., adding phenoxyethanol, peach resin extract, sunflower seed extract and purslane extract and stirring evenly to obtain an extract composition cream.

[0085] Comparative Example 1

[0086] A blank cream, compared with Example 4, differs in that it does not contain peach resin extract, ambrette fruit extract and purslane extract, and has a different content of purified water. The blank cream is specifically prepared from the following raw materials in percentage by weight: 8% caprylic capric triglyceride, 5% coconut oil caprylate / caprate, 2% cetearyl alcohol, 2% steareth-2, 2% steareth-21, 5% propylene glycol, 0.15% carbomer, 0.15% triethanolamine, 0.5% phenoxyethanol and the balance purified water.

[0087] Example 5

[0088] Irritation test

[0089] According to the requirements of human skin patch test in the "Technical Specifications for Safety of Cosmetics (2015 Edition)", 35 test personnel were selected according to the subject selection criteria to conduct patch test, and 20-25 mg of the creams prepared in Example 4 and Comparative Example 1 were respectively taken and placed in qualified patch testers, and applied to the inside of the subject's arm for 24 hours. According to the "Skin Reaction Grading Standard for Skin Closure Patch Test", the skin reaction was observed and recorded 30 minutes, 24 hours, and 48 hours after the patch tester was removed. The skin reaction grading standard for skin closure patch test is shown in Table 4 below, and the experimental results are shown in Table 5 below.

[0090] Table 4 Skin occlusive patch test skin reaction grading standard

[0091]

[0092] Table 5 Human skin patch test results

[0093]

[0094] As can be seen from Table 5, for 35 subjects, the skin reactions to the blank cream of Comparative Example 1 and the extract composition cream of Example 4 were negative at 30 min, 24 h, and 48 h after removing the patch tester, indicating that the extract composition of the present invention is mild, non-irritating, and has high safety.

[0095] Example 6

[0096] Skin feel test

[0097] Thirty volunteers were selected to use the creams of Example 4 and Comparative Example 1 on their faces, and the spreadability, absorbability, greasiness, and moisturizing properties of the creams were evaluated and scored. The test results were scored on a 10-point scale, with the best being 10 points and the worst being 0 points. The higher the score, the better the spreadability, faster the absorption, lower the greasiness, and higher the moisturizing degree of the cream. The scoring results of each item are shown in Table 6.

[0098] Table 6 Scoring results of skin feel test

[0099] Test Items Comparative Example 1 Example 1 Spreadability 5.2±0.4 <![CDATA[8.7±0.7 * > Absorbability 6.5±0.6 <![CDATA[7.8±0.6 * > Stickiness 4.6±0.5 <![CDATA[7.5±0.5 * > Moisturizing Property 5.5±0.5 <![CDATA[8.1±0.6 * >

[0100] Note: Compared with Comparative Example 1, * P < 0.05.

[0101] As can be seen from Table 6, compared with the blank cream of Comparative Example 1, the cream of Example 4 added with the extract composition containing peach resin extract, okra fruit extract, and purslane extract has significantly better spreadability, significantly reduced greasiness, significantly enhanced moisturizing property, and faster absorption, and there are significant differences in each index, indicating that the extract composition can improve the silkiness of skin care products, reduce the greasiness of skin care products, improve the moisturizing property of skin care products, and accelerate the absorption rate of skin care products.

[0102] Comparative Examples 2-4 and Example 7

[0103] Compared with Comparative Example 1, Comparative Example 2 added peach resin extract, and except for the amount of purified water used, the weight percentage contents of other raw materials were the same;

[0104] Compared with Comparative Example 1, Comparative Example 3 added okra fruit extract, and except for the amount of purified water used, the weight percentage contents of other raw materials were the same;

[0105] Compared with Comparative Example 1, Comparative Example 4 added purslane extract, and except for the amount of purified water used, the weight percentage contents of other raw materials were the same;

[0106] Compared with Comparative Example 1, Example 7 added peach resin extract, okra fruit extract, and purslane extract, and except for the amount of purified water used, the weight percentage contents of other raw materials were the same.

[0107] The specific concentrations of peach resin extract, okra fruit extract and purslane extract in the creams of Comparative Examples 1-4 and Example 7 are listed in Table 7.

[0108] Table 7 Weight percentage of extracts in different creams

[0109] Cream Peach Resin Extract Okra Fruit Extract Purslane Extract Comparative Example 1 0 0 0 Comparative Example 2 0.4％ 0 0 Comparative Example 3 0 0.4％ 0 Comparative Example 4 0 0 0.4％ Example 7 0.1％ 0.1％ 0.2％

[0110] Example 8

[0111] Skin test

[0112] 1. Test samples: the creams of Comparative Examples 1-4 and Example 7.

[0113] 2. Test instruments: Skin Moisture Content Tester CM825 and Skin Moisture Loss Rate Tester TM300 produced by CK Company in Germany.

[0114] 3. Thirty volunteers with healthy skin, no history of cosmetic allergy and aged 20-35 years were selected as subjects. The inner sides of the left and right arms of the subjects, 5 cm from the base of the palm, were selected as the test sites, and the test area was (5×5) cm 2 . Equal amounts of the test samples were applied to the test sites. Before the test, the inner sides of the forearms of the subjects were cleaned uniformly and they sat quietly for 30 min in a constant temperature and humidity environment (temperature (20±2)°C, relative humidity 50%±10%). The initial values of the skin water content of each test site before applying the sample and the test values of the skin water content of each test site at 0.5 h, 1 h, 2 h, 4 h, 6 h, 8 h and 12 h after using the test sample were measured. Each measurement was repeated 5 times in parallel and the average value was taken. The skin water content improvement rate was calculated according to the following formula, and the results are shown in Table 8 and Figure 4 as follows.

[0115] Skin water content improvement rate (%) = (skin water content 测试值 - skin water content 起始值 ) / skin water content 起始值 ×100%;

[0116] wherein, skin water content 起始值 is the skin water content before applying the sample, and skin water content 测试值 is the skin water content at different time points after applying the sample.

[0117] Table 8 Skin water content improvement rate (%) after treatment with different creams

[0118] Time Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Example 7 0.5h 75.1±5.4 <![CDATA[101.7±8.4 a > <![CDATA[92.5±6.6 a > <![CDATA[88.9±6.5 a > <![CDATA[142±9.4 ab > 1h 63.7±5 87.9±6.6 85.8±6.1 80.6±5.8 135.7±8.5 2h 48.9±4.8 79.8±6.1 80.4±5.8 73.2±5.2 122.5±8.1 4h 37.5±4.4 68.9±7.1 57.5±5.1 57.6±5.3 103.6±7.8 6h 29.2±3.8 51.3±5.5 45.5±4.5 42.2±5.1 84.6±6.9 8h 19.4±3.7 37.8±5.1 37.4±4.7 34±5.3 60.7±6.5 12h 7.7±3.3 <![CDATA[24.8±4.2 * > <![CDATA[19.3±4.6 * > <![CDATA[16.7±4.5 * > <![CDATA[34.3±5.8 *# >

[0119] Note: At 0.5 h, compared with Comparative Example 1, a P<0.05; at 0.5 h, compared with Comparative Example 2, bP < 0.05; At 12 h, compared with Comparative Example 1, * P < 0.05; At 12 h, compared with Comparative Example 2, # P < 0.05.

[0120] From Table 8 and Figure 4 it can be seen that the moisturizing trends of the creams in Comparative Examples 1 - 4 and Example 7 are basically the same, and the moisturizing effects of the creams in Comparative Examples 2 - 4 are much better than the blank cream in Comparative Example 1. The total content of active ingredients in the creams prepared in Comparative Examples 2 - 4 and Example 7 is the same. The immediate moisturizing effect after 0.5 h and the long - term moisturizing effect after 12 h of the cream prepared in Example 7 are significantly better than the single - active - ingredient creams prepared in Comparative Examples 2 - 4, and there are significant differences. After applying the cream of the composition of Example 7 for 0.5 h, the skin water content increased by 142%, and after 12 h, the skin water content still increased by 34.3%. While after applying the single creams of peach resin extract (Comparative Example 2), okra fruit extract (Comparative Example 3), and purslane extract (Comparative Example 4) with the same total concentration for 0.5 h, the skin water content only increased by 101.7%, 92.5%, and 88.9% respectively, and after 12 h, the skin water content increased by only 24.8%, 19.3%, and 16.7% respectively. In summary, it shows that the extract composition of the present invention has excellent immediate and long - term moisturizing effects, and peach resin extract, okra fruit extract, and purslane extract have a synergistic effect.

[0121] 4. On the basis of Step 3, apply corresponding equal amounts of test samples on the same test area every morning and evening within one month, and measure the transepidermal water loss rate (TEWL) on the 1st, 5th, 10th, 15th, 20th, 25th, 28th, and 30th days. The test environment and method are the same as those in Step 3. Each time, measure 5 times in parallel and take the average value. Calculate the improvement rate of transepidermal water loss rate according to the following formula, and the results are shown in Table 9 and Figure 5 as follows.

[0122] Improvement rate of transepidermal water loss rate (%) = (TEWL 起始值 - TEWL 测试值 ) / TEWL 起始值 × 100%;

[0123] wherein, TEWL 起始值 is the transepidermal water loss rate of the skin before applying the sample, and TEWL 测试值 is the transepidermal water loss rate of the skin at different time points after applying the sample.

[0124] Table 9 Improvement rate of transepidermal water loss rate (%) after different cream treatments

[0125] Time Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Example 7 1 day 0.23±0.02 0.84±0.06 0.71±0.06 0.66±0.08 1.23±0.16 5 days 0.55±0.06 1.57±0.12 1.33±0.38 1.05±0.13 3.95±0.42 10 days 1.46±0.09 3.44±0.29 3.16±0.41 2.87±0.31 5.76±0.63 15 days 2.83±0.21 5.78±0.64 5.48±0.59 4.91±0.53 8.27±0.85 20 days 5.57±0.46 9.19±0.88 8.52±0.92 8.07±0.74 14.63±1.59 25 days 6.85±0.71 11.83±1.23 10.94±1.66 10.14±1.02 17.33±1.88 28 days 9.24±0.96 16.55±1.78 15.63±1.95 13.26±1.45 21.46±2.22 30 days 9.97±1.43 <![CDATA[16.92±1.81 * > <![CDATA[16.15±1.82 * > <![CDATA[13.59±1.58 * > <![CDATA[21.97±2.05 *# >

[0126] Note: At 30 days, compared with Comparative Example 1,* P < 0.05; At 30 days, compared with Comparative Example 2, # P < 0.05.

[0127] The trans-epidermal water loss rate (TEWL) reflects the amount of water evaporated from the skin surface and is an important indicator of skin barrier function. From Table 9 and Figure 5 it can be seen that as time increases, the trans-epidermal water loss rates after using the creams of Comparative Examples 1-4 and Example 7 have all improved, and the improvement speeds of Comparative Examples 2-4 and Example 7 are significantly higher than that of the blank cream of Example 1. The total content of active ingredients in the creams prepared in Comparative Examples 2-4 and Example 7 is the same. After 30 days of use, the trans-epidermal water loss rate of the composition cream prepared in Example 7 decreased by 21.97%, which is significantly higher than the single active ingredient creams (16.92%, 16.15%, 13.59%) prepared in Comparative Examples 2-4, showing a significant difference. This indicates that the combined use of peach resin extract, okra fruit extract, and purslane extract has a better effect on repairing the keratin barrier than a single ingredient, showing a synergistic effect.

[0128] Example 9

[0129] Skin moisturizing, chapping prevention, and eczema alleviation effect test

[0130] 1. Test samples: The creams of Comparative Examples 1-4 and Example 7.

[0131] 2. Test instrument: Skin moisture content tester CM825 produced by CK Company, Germany.

[0132] 3. Fifty healthy infants and children aged 0-12 years were selected as volunteers. The volunteers were divided into 5 groups, with 10 people in each group. The test samples were applied morning and evening in a dry winter environment for 30 days. The chapping prevention and eczema alleviation effects were scored on a 10-point scale, with the best being 10 points and the worst being 0 points. The chapping prevention effect and eczema alleviation effect were scored according to the self-report of the test subjects or guardians. The moisturizing effect was evaluated by measuring the facial moisture content using a skin moisture tester (the skin moisture content increase rate of 0-10% is 1 point, 10-20% is 2 points, and so on). The evaluation results are shown in Table 10.

[0133] Table 10 Test results of skin moisturizing, chapping prevention, and eczema alleviation effects of test samples

[0134]

[0135]

[0136] Note: Compared with Comparative Example 1, * P < 0.05; Compared with Comparative Example 2, #P < 0.05; compared with Comparative Example 4, & P < 0.05.

[0137] As can be seen from Table 10, the moisturizing, chapping prevention, and eczema alleviation effects of the creams of Comparative Examples 2-4 and Example 7 on infants and children are significantly better than those of the blank cream of Comparative Example 1. The total content of active ingredients in the creams prepared in Comparative Examples 2-4 and Example 7 is the same. After 30 days of use, the moisturizing, chapping prevention, and eczema alleviation effects of the cream composition prepared in Example 7 are significantly better than those of the single active ingredient creams prepared in Comparative Examples 2-4, and there are significant differences. It shows that the combined use of peach resin extract, okra fruit extract, and purslane extract has a better effect on infantile eczema and skin dryness and chapping than a single ingredient, and has a synergistic effect.

[0138] According to the above content, it can be seen that the extract composition provided by the present invention is mild, non-irritating, has high safety, has excellent moisturizing, repairing, and soothing effects, and can improve the skin feel experience of skin care products.

[0139] Although the above embodiments have described the present invention in detail, they are only a part of the embodiments of the present invention, not all of them. People can also obtain other embodiments based on these embodiments without creative efforts, and these embodiments all fall within the protection scope of the present invention.

Claims

1. An extract composition, characterized in that, It includes peach resin extract, Abelmoschus esculentus fruit extract and Portulaca oleracea extract; In the extract composition, the mass ratio of the peach resin extract, the Abelmoschus esculentus fruit extract and the Portulaca oleracea extract is (1-20):(1-20):(1-50).

2. The extract composition according to claim 1, wherein In the extract composition, the mass ratio of the peach resin extract, the Abelmoschus esculentus fruit extract and the Portulaca oleracea extract is 1:1:(1-10).

3. The extract composition according to claim 1 or 2, characterized in that, In the extract composition, the mass ratio of the peach resin extract, the Abelmoschus esculentus fruit extract and the Portulaca oleracea extract is 1:1:

2.

4. Use of the extract composition according to any one of claims 1 to 3 in the preparation of cosmetics.

5. The application according to claim 4, wherein The cosmetics are cosmetics having one or more of the effects of moisturizing, soothing and repairing.

6. The application according to claim 4, wherein The mass percentage content of the extract composition in the cosmetics is ≥0.1%.

7. The application according to claim 4, wherein The mass percentage content of the extract composition in the cosmetics is 0.1%-5%.

8. The application according to any one of claims 4 to 7, characterized in that The dosage forms of the cosmetics include one or more of cream, lotion, essence, toner, gel, facial mask, shampoo, conditioner, facial cleanser and body wash.

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