Anti-aging composition for promoting communication between skin cells and application of anti-aging composition

Abstract

The invention relates to an anti-aging composition for promoting skin intercellular communication and application thereof, and the composition comprises the following components: a camellia nitidissima flower extract, a bacillus fermentation product, an European basswood extract and safflower glucoside, the weight ratio of the golden camellia flower extract to the bacillus fermentation product to the European basswood extract to the safflower glucoside is (1-10): (1-8): (1-6): (0.1-1). The anti-aging composition provided by the invention can promote the activity of skin communication cells, enhance intercellular communication and interaction, give full play to the biological function of cells, and achieve the anti-aging effect; the composition has the effects of removing wrinkles, tightening, smoothing and repairing, can inhibit damage of cortisol to skin barriers, achieves the effects of repairing and relieving mood pressure, and can achieve the optimal effects of removing wrinkles, tightening, smoothing and repairing due to mutual synergism of the components.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and particularly relates to an anti-aging composition for promoting skin cell communication and its application. Background Art

[0002] Dry and sensitive skin is prone to problems such as water loss and over-stimulation reactions due to its weak skin barrier function, which makes dry and sensitive skin more susceptible to the influence of external environments (such as climate change, pollution, etc.) and endogenous factors (such as the aging process). Research shows that the repair of the skin barrier is crucial for anti-aging. By using skin care products containing ingredients such as ceramides, hyaluronic acid, and squalene, it can effectively help enhance the skin barrier and maintain skin hydration, thereby delaying the appearance of aging. Although existing anti-aging research has made progress in many aspects, there are still some limitations, mainly reflected in the following aspects:

[0003] On the one hand, currently, most anti-aging research focuses on improving skin barrier function, antioxidant properties, and cell repair, but relatively little research has been done on cell communication. Cell communication plays an important role in the health and aging process of the skin. The skin is not only composed of different types of cells such as epidermal cells and dermal cells, which work together through cell signaling, the interaction between the matrix and cells, etc. to maintain the structure and function of the skin; during the skin aging process, the signal transmission function between cells may be damaged. With aging, the communication ability between epidermal cells and dermal cells decreases, which leads to a decrease in skin hydration, a reduction in collagen synthesis, etc.

[0004] On the other hand, although many anti-aging ingredients have been widely used, research on their long-term effects and safety is still insufficient. Especially for dry and sensitive skin, excessive active ingredients may cause skin irritation and allergic reactions, so more clinical studies on long-term use are needed to evaluate their effects and safety. Summary of the Invention

[0005] The purpose of the present invention is to overcome the deficiencies of the prior art and provide an anti-aging composition for promoting skin cell communication and its application.

[0006] To achieve the above purpose, the technical solution adopted by the present invention is as follows:

[0007] In the first aspect, the present invention provides an anti-aging composition for promoting skin cell communication, comprising the following components: camellia nitidissima leaf extract, bacillus ferment, tilia europaea extract, and rubroglucosides, and the weight ratio of the camellia nitidissima leaf extract, bacillus ferment, tilia europaea extract, and rubroglucosides is (1 - 10):(1 - 8):(1 - 6):(0.1 - 1).

[0008] The flower extract of Camellia chrysantha mainly contains flavonoids, polyphenols and flavonoid compounds, which have strong antioxidant effects. Its molecular structure includes the benzene ring structure of polyphenols, which can react with free radicals through its phenolic hydroxyl groups, inhibit oxidation reactions, and slow down skin aging. Secondly, the rich polyphenols bind to the extracellular matrix on the skin surface, promoting the interaction between epidermal cells and dermal components, thereby enhancing the skin barrier function. The flower extract of Camellia chrysantha helps to activate the TGF-β pathway, promote the generation of collagen by fibroblasts in the skin, and improve skin elasticity and slow down the aging process through the regulation of intercellular signals.

[0009] The Bacillus fermentation product contains a large amount of proteins, amino acids and active enzymes, which improve the skin repair process through enzymatic reactions. Its structure is diverse, including natural amino acid chains, peptide structures and enzyme molecules, which can effectively activate the repair and regeneration functions of cells. The enzyme components can promote the renewal and regeneration of keratinocytes by decomposing dead skin cells on the skin surface, thereby enhancing the skin barrier function. The amino acid components improve the nutrient transfer and signal communication between cells through their interactions with skin cells. By improving the stratum corneum structure of the skin, the Bacillus fermentation product helps to enhance the cooperation between epidermal cells and the dermis, regulate cytokines such as VEGF, and promote skin microcirculation, thus slowing down the aging process.

[0010] The extract of Tilia europaea contains rich flavonoids, polysaccharides and organic acids. These components are used in skin care products to enhance the skin's antioxidant capacity, soothe skin inflammation, and promote skin repair and protection. As a flavonoid compound, quercetin can reduce skin oxidative damage by inhibiting the generation of free radicals, and reduce the inflammatory response by regulating the NF-κB pathway and MAPK pathway. The antioxidant effect of flavonoids can reduce cell damage caused by oxidative stress during the aging process. The polysaccharide components in the Tilia extract can promote the interaction between skin cells, enhance the skin's hydration and repair functions, and effectively relieve skin problems caused by dryness and aging.

[0011] Safflower Glucoside is a glycoside structure formed by the combination of glucose molecules and active ingredients in safflower, which helps it to be more easily hydrolyzed and release active ingredients in the skin, enhance the absorption of antioxidant components by skin cells, and slow down the aging process. Through its flavonoid components, Safflower Glucoside can regulate the intercellular antioxidant pathway, inhibit the oxidative damage of skin cells, and improve the firmness and elasticity of the skin.

[0012] The composition of the present invention synergistically enhances the antioxidant and anti-aging effects through four components: Camellia nitidissima Chi flower extract, Bacillus fermentation product, Tilia europaea extract, and carthamin glucoside. Moreover, by regulating the signal transduction between cells, it improves the self-repair ability and barrier function of the skin, thus achieving a synergistic anti-aging effect. Not only that, the combination of the four substances can also inhibit stress hormones, effectively improve the damage to the skin caused by negative emotions, repair the barrier, and enhance the overall health of the skin.

[0013] Preferably, the weight ratio of the Camellia nitidissima Chi flower extract, Bacillus fermentation product, Tilia europaea extract, and carthamin glucoside is (1-8):(1-5):(1-4):(0.1-0.5).

[0014] Preferably, the weight ratio of the Camellia nitidissima Chi flower extract, Bacillus fermentation product, Tilia europaea extract, and carthamin glucoside is (1-5):(1-3):(1-2):(0.1-0.3).

[0015] In a second aspect, the present invention provides the use of the anti-aging composition for promoting skin cell communication in the first aspect in the preparation of cosmetics.

[0016] Preferably, the cosmetics are lotion, emulsion, cream, mask, essence, or spray, and the addition amount of the composition is 1%-3% of the total weight of the cosmetics.

[0017] In a third aspect, the present invention provides an essence, which comprises the following raw materials in weight percentages: 1%-3% of the anti-aging composition described in the first aspect, 0.05%-0.5% of a thickener, 3%-20% of a humectant, 0.2%-5% of a preservative, 0.05%-0.1% of a pH regulator, and 0.01%-1% of a fragrance, with the balance being deionized water.

[0018] Preferably, the thickener comprises at least one of xanthan gum, Carbopol 980, AVC, and high molecular cellulose.

[0019] Preferably, the humectant comprises at least one of allantoin, sodium polyacrylate, hydrogenated lecithin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,2-butanediol, glycerol, pullulan, and ceramide.

[0020] Preferably, the pH regulator comprises at least one of arginine, sodium hydroxide, and EDTA-2Na.

[0021] Preferably, the preservative comprises at least one of 1,3-propanediol, 1,2-hexanediol, and p-hydroxyacetophenone.

[0022] Fourthly, the present invention provides a preparation method of the essence liquid described in the third aspect, including the following steps:

[0023] S1. Mix a humectant, a thickener and a part of deionized water, homogenize at 75 - 85 °C, then add a preservative and stir evenly to obtain a mixture;

[0024] S2. Wait until the temperature of the mixture in S1 drops to 35 - 45 °C, add each component of the anti-aging composition, essence and the remaining deionized water, stir evenly, and finally add a pH regulator to adjust the pH to obtain the essence liquid.

[0025] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0026] The components of the anti-aging composition of the present invention are extracts of Camellia nitidissima flowers, Bacillus fermentation products, extracts of Tilia europaea and ruberythric glycoside. Their synergy can promote the vitality of skin communication cells, strengthen cell - to - cell communication and interaction, give full play to cell biological functions, and achieve the anti-aging effect; and inhibit the damage of cortisol to the skin barrier, achieving the effects of repair and soothing emotional stress. The mutual synergy of these four components can achieve the best effects of wrinkle removal, firming, smoothing and repair. Description of the Drawings

[0027] Figure 1 It is a comparison diagram of facial wrinkles before and after using the essence liquid in Application Example 1;

[0028] Figure 2 It is a comparison diagram of facial wrinkles before and after using the essence liquid in Comparative Application Example 5. Detailed Embodiments

[0029] To better illustrate the purpose, technical solution and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0030] The sources of the materials used in the following embodiments and comparative examples are as follows:

[0031] Extract of Camellia nitidissima flowers: The manufacturer is SKEYNDOR (Xiamen) Biotechnology Co., Ltd.;

[0032] Bacillus fermentation products: The manufacturer is Guangzhou Youke Biotechnology Co., Ltd.;

[0033] Extract of Tilia europaea: The manufacturer is Haosite New Materials Technology (Suzhou) Co., Ltd.;

[0034] Ruberythric glycoside: The manufacturer is Jingcui Tiancheng Pharmaceutical Technology Co., Ltd.

[0035] Example 1

[0036] An anti-aging composition for promoting communication between skin cells, comprising camellia nitidissima leaf extract, bacillus ferment, tilia europaea extract and rubrofuscoside in a weight ratio of 4:2:1.3:0.2, and the total mass fraction of the camellia nitidissima leaf extract, bacillus ferment, tilia europaea extract and rubrofuscoside is 100 parts.

[0037] Example 2

[0038] An anti-aging composition for promoting communication between skin cells, comprising camellia nitidissima leaf extract, bacillus ferment, tilia europaea extract and rubrofuscoside in a weight ratio of 1:3:2:0.3, and the total mass fraction of the camellia nitidissima leaf extract, bacillus ferment, tilia europaea extract and rubrofuscoside is 100 parts.

[0039] Example 3

[0040] An anti-aging composition for promoting communication between skin cells, comprising camellia nitidissima leaf extract, bacillus ferment, tilia europaea extract and rubrofuscoside in a weight ratio of 5:1:1:0.1, and the total mass fraction of the camellia nitidissima leaf extract, bacillus ferment, tilia europaea extract and rubrofuscoside is 100 parts.

[0041] Example 4

[0042] An anti-aging composition for promoting communication between skin cells, comprising camellia nitidissima leaf extract, bacillus ferment, tilia europaea extract and rubrofuscoside in a weight ratio of 1:1:4:0.5, and the total mass fraction of the camellia nitidissima leaf extract, bacillus ferment, tilia europaea extract and rubrofuscoside is 100 parts.

[0043] Example 5

[0044] An anti-aging composition for promoting communication between skin cells, comprising camellia nitidissima leaf extract, bacillus ferment, tilia europaea extract and rubrofuscoside in a weight ratio of 8:5:1:0.1, and the total mass fraction of the camellia nitidissima leaf extract, bacillus ferment, tilia europaea extract and rubrofuscoside is 100 parts.

[0045] Example 6

[0046] An anti-aging composition for promoting communication between skin cells, comprising camellia nitidissima leaf extract, bacillus ferment, tilia europaea extract and rubrofuscoside in a weight ratio of 1:8:6:0.1, and the total mass fraction of the camellia nitidissima leaf extract, bacillus ferment, tilia europaea extract and rubrofuscoside is 100 parts.

[0047] Example 7

[0048] An anti-aging composition for promoting skin cell communication, comprising camellia chrysantha flower extract, bacillus fermentation product, tilia europaea extract and rubrofuscoside in a weight ratio of 10:1:0.1:0.01, and the total mass fraction of the camellia chrysantha flower extract, bacillus fermentation product, tilia europaea extract and rubrofuscoside is 100 parts.

[0049] Comparative Example 1

[0050] The difference between Comparative Example 1 and Example 1 is that: the composition does not add camellia chrysantha flower extract, and the missing amount is supplemented with bacillus fermentation product, tilia europaea extract and rubrofuscoside in a mass ratio of 2:1.3:0.2.

[0051] Comparative Example 2

[0052] The difference between Comparative Example 2 and Example 1 is that: the composition does not add bacillus fermentation product, and the missing amount is supplemented with camellia chrysantha flower extract, tilia europaea extract and rubrofuscoside in a weight ratio of 4:1.3:0.2.

[0053] Comparative Example 3

[0054] The difference between Comparative Example 3 and Example 1 is that: the composition does not add tilia europaea extract, and the missing amount is supplemented with camellia chrysantha flower extract, bacillus fermentation product and rubrofuscoside in a weight ratio of 4:2:0.2.

[0055] Comparative Example 4

[0056] The difference between Comparative Example 4 and Example 1 is that: the composition does not add rubrofuscoside, and the missing amount is supplemented with camellia chrysantha flower extract, bacillus fermentation product and tilia europaea extract in a weight ratio of 4:2:1.3.

[0057] Test Example 1: Evaluation of the effect of samples on the viability of human skin fibroblasts (HSF) based on the MTT method

[0058] 1. Materials and reagents

[0059] Cell line: Human skin fibroblasts (HSF), purchased from Kunming Cell Bank, Chinese Academy of Sciences.

[0060] Medium: High-glucose DMEM medium (1×, containing 10% fetal bovine serum FBS, Gibco).

[0061] Reagents: Phosphate buffer (PBS, Solarbio), thiazolyl blue (MTT, Yuanye Bio-Technology), dimethyl sulfoxide (DMSO, Tianjin Damao).

[0062] Test samples: Anti-aging compositions of Examples 1-7 and Comparative Examples 1-4, and the compositions were formulated into a test sample solution with a concentration of 0.05% (m / m) using fresh culture medium.

[0063] 2. Experimental procedures

[0064] 2.1 Cell seeding and culture

[0065] Inoculate the HSF cell suspension into a 96-well cell culture plate, and adjust the seeding density per well to approximately 5×10 3 cells (the specific density needs to be optimized according to the cell growth rate). Place the culture plate in an incubator at 37°C and 5% CO2 for 18-24 h until the cells adhere to the wall.

[0066] 2.2 Sample treatment

[0067] Discard the original culture medium in the wells, gently add PBS along the well wall to wash once, and avoid cell detachment.

[0068] Experimental grouping:

[0069] Sample group: Add 100 μL of the test sample solution with a concentration of 0.05% (m / m) to each well.

[0070] Blank control group: Add 100 μL of fresh culture medium (without the test sample) to each well.

[0071] 2.3 MTT assay for cell viability

[0072] Place the culture plates containing the sample group and the blank control group back into the incubator and incubate for 24±1 h. After incubation, add 20 μL of MTT solution (5 mg / mL, prepared with PBS) to each well and mix gently. Continue to culture for 3-4 h to allow viable cells to reduce MTT to formazan crystals. Carefully aspirate the liquid in the wells, avoiding aspirating the formazan crystals, add 100 μL of DMSO to each well, and place it on an oscillator and shake for 10-15 min to fully dissolve the crystals.

[0073] 2.4 Absorbance measurement and calculation

[0074] Use a microplate reader to measure the absorbance (OD value) of each well at a wavelength of 570 nm, and set the well without cells (containing only culture medium and MTT) as the blank well for zero adjustment.

[0075] Formula for calculating the viability of HSF cells:

[0076] HSF cell viability = (OD value 样品孔 - OD value 调零 ) / (OD value 空白对照孔 - OD value 调零)×100%. The cell viability data of HSF cells reflect the proliferation status of HSF cells. A higher cell viability indicates that the cells have a stronger proliferation ability and can grow and divide rapidly, which is crucial for maintaining the normal renewal and repair functions of skin tissues. This is because HSF cells have a strong ability to synthesize collagen and elastin, and promote wound healing and skin elasticity through proliferation and tissue repair. The specific data are shown in Table 1.

[0077] Test Example 2: Evaluation of the improvement effect of the sample on cortisol-induced HaCaT cell damage based on the MTT method

[0078] 1. Experimental materials

[0079] Phosphate buffer solution (PBS, Solarbio, Shanghai), thiazolyl blue (MTT, Yuanye Bio-Technology), dimethyl sulfoxide (DMSO, Damao, Tianjin), high-glucose medium 1×DMEM (Gibco), fetal bovine serum (FBS, Gibco), human keratinocyte HaCaT (Shanghai Yaji Biotechnology Co., Ltd.), cortisol (MedChemExpress, HY-N0583).

[0080] 2. Experimental conditions

[0081] Incubator temperature: 37 ± 1°C; humidity: 90 ± 5%; carbon dioxide concentration: 5 ± 1%.

[0082] 3. Experimental steps

[0083] 3.1 Cell seeding: Inoculate the human keratinocyte HaCaT suspension into a 96-well cell culture plate at a density of 2000 cells per well.

[0084] 3.2 Culture medium addition: Add 100 μL of DMEM medium (containing 10% fetal bovine serum and 1% penicillin / streptomycin) to each well and resuscitate and culture for 24 hours.

[0085] 3.3 Grouping treatment:

[0086] Blank group: After discarding the supernatant, add 100 μL of DMEM medium.

[0087] Model group: Add 100 μL of DMEM medium containing 100 nM cortisol.

[0088] Sample group: Add 100 μL of medium containing 100 nM cortisol and 0.05% (m / m) corresponding sample solution; the sample solutions correspond to the anti-aging compositions of Examples 1-7 and Comparative Examples 1-4 respectively.

[0089] 4. Culture and detection: After culturing each group for 24 hours, add 20 μL of MTT solution to each well and incubate in the incubator for 3-4 hours.

[0090] 5. Absorbance measurement: Remove the liquid in the wells, add 100 μL of DMSO to each well, and after oscillating for 10 - 15 minutes, measure the absorbance OD value at a wavelength of 570 nm using a microplate reader.

[0091] 6. Calculation of cell viability: Calculate the viability of HaCaT cells and the improvement rate of HaCaT cell viability according to the formula:

[0092] Viability of HaCaT cells (%) = (OD value of sample well - OD value of zero adjustment) / (OD value of blank control well - OD value of zero adjustment) × 100%.

[0093] Improvement rate of HaCaT cell viability (%) = (Cell viability of sample group - Cell viability of model group) / Cell viability of model group × 100%. The improvement rate is used to evaluate the repair effect of the sample on cortisol damage. A positive value indicates improvement, a negative value indicates aggravated damage, and the improvement rate of cell viability reflects the repair ability of the sample on cortisol-induced damage to HaCaT cells. A higher improvement rate means that the sample can more effectively reduce the damage caused by cortisol to cells, promote cells to restore normal functions and viability, and thus protect skin cells from stress damage to a certain extent. See Table 1 for details.

[0094] Table 1 Test data of samples in each group

[0095] Group Fibroblast viability (%) Improvement rate of keratinocyte viability (%) Example 1 120.4 62.7 Example 2 116.1 60.9 Example 3 115.7 60.7 Example 4 112.0 58.5 Example 5 113.6 58.7 Example 6 104.1 55.8 Example 7 103.6 55.5 Comparative Example 1 87.1 17.4 Comparative Example 2 81.6 18.7 Comparative Example 3 83.7 18.9 Comparative Example 4 87.5 19.2

[0096] As can be seen from Table 1, combining the data of Example 1 and Comparative Examples 1 - 4, it can be known that the composition of Example 1 with the four components of Camellia nitidissima flower extract, Bacillus fermentation product, Tilia europaea extract, and ruberythrin glycoside compounded has a significantly improved effect on the viability of fibroblasts and the improvement rate of keratinocyte viability compared with the compositions of Comparative Examples 1 - 4. This shows that the four components of Camellia nitidissima flower extract, Bacillus fermentation product, Tilia europaea extract, and ruberythrin glycoside can synergistically promote the proliferation of HSF cells and improve the repair ability of cortisol-induced damage to HaCaT cells. This may be because the four components improve the communication and interaction between skin cells, thereby enhancing the viability of HSF cells, overall improving skin health, achieving anti-aging effects such as lifting, firming, and filling, and having the effect of inhibiting stress sources and improving the damage of emotional stress hormones to the skin barrier.

[0097] Combining the data of Examples 1 - 7, it can be known that when the weight ratio of Camellia nitidissima flower extract, Bacillus fermentation product, Tilia europaea extract, and ruberythrin glycoside is (1 - 5):(1 - 3):(1 - 2):(0.1 - 0.3), the composition has a better effect on improving the viability of fibroblasts and the improvement rate of keratinocyte viability.

[0098] Application Examples 1-7 and Comparative Application Examples 1-4

[0099] The compositions of Examples 1-7 and Comparative Examples 1-4 were added to the essence at a concentration of 3 wt% respectively to obtain the essences of Application Examples 1-7 and Comparative Application Examples 1-4. The formulations are shown in Table 2.

[0100] The preparation method of the essences of Application Examples 1-7 and Comparative Application Examples 1-4 includes the following steps:

[0101] The preparation method of the essence includes the following steps:

[0102] S1. Mix the humectant, thickener and 1 / 2 amount of deionized water, homogenize evenly at 80 °C, then add the preservative and stir evenly to obtain a mixture;

[0103] S2. Wait for the temperature of the mixture in S1 to drop to 40 °C, add each component in the anti-aging composition, essence and the remaining deionized water, stir evenly, and finally add a pH regulator to adjust the pH to 6 to obtain the essence.

[0104] Table 2 Formulations of the essences of Application Examples 1-7 and Comparative Application Examples 1-4

[0105]

[0106]

[0107] Comparative Application Example 5

[0108] The essence of Comparative Application Example 5 does not add the anti-aging composition, and an equal amount of deionized water is used instead of the composition. The preparation method is the same as that of Application Example 1.

[0109] Test Example 3: Efficacy test of the essence

[0110] This test example verifies the effects of the essences of Application Examples 1-7 and Comparative Application Examples 1-5 on anti-aging and repairing the skin barrier. The experimental data are shown in Table 3.

[0111] According to the "Technical Specifications for Cosmetics Safety" (2015), 60 volunteers aged 18 - 45 with dry and sensitive skin (lactic acid stinging score > 3, skin moisture content 20 C.U - 50 C.U) were selected for the test. They were randomly divided into 12 groups, with 5 people in each group. Under normal circumstances, the volunteers used the product on the whole face. The volunteers took 1 mL of the essence and applied it to the face, and then massaged it until absorbed. The volunteers were in an air-conditioned room with a temperature of 21 ± 1 °C and a humidity of 50 ± 10%. Instruments were used to quantify and photograph the improvement of facial indicators. The test time was the indicators before use, 7 days after use, and 28 days after use: The Primos CR 5.8E three-dimensional skin analyzer was selected to test wrinkles, the Cutometer 2mm MPA 580 skin elasticity tester was selected to test elasticity, and the TewameterTM Hex transdermal water loss meter was selected to measure the transdermal water loss (TEWL) value.

[0112] The calculation formulas are as follows:

[0113] Wrinkle improvement rate = (wrinkle 使用前 - wrinkle 使用后 ) / wrinkle 使用前 × 100%;

[0114] Elasticity improvement rate = (elasticity 使用后 - elasticity 使用前 ) / elasticity 使用前 × 100%.

[0115] TEWL value improvement rate = (TEWL value 使用后 - TEWL value 使用前 ) / TEWL value 使用前 × 100%;

[0116] The calculation results are shown in Table 2.

[0117] Table 2 Human efficacy test data of essence samples in each group

[0118]

[0119]

[0120] The anti-aging effect of the application example is represented by the wrinkle improvement rate and the elasticity improvement rate, and the repair effect on barrier damage is represented by the TEWL value improvement rate.

[0121] It can be seen from Figure 1-2 that the essence of Application Example 1 has significantly improved the wrinkle improvement effect of the subjects after 28 days of use compared with Comparative Application Example 5, indicating that the essence added with the anti-aging composition has good anti-aging and repair effects.

[0122] As can be seen from the data of Application Example 1 and Comparative Application Examples 1-4 in Table 2, the anti-aging and barrier repair effects of the composition of Example 1, which is a compound of Camellia nitidissima extract, Bacillus fermentation product, Tilia europaea extract and ruberythrin, are significantly improved compared with those of the compositions of Comparative Examples 1-4, indicating that these four components, namely Camellia nitidissima extract, Bacillus fermentation product, Tilia europaea extract and ruberythrin, can synergistically achieve the effects of barrier repair and anti-aging.

[0123] Combined with the data of Application Examples 1-7, it can be seen that when the weight ratio of Camellia nitidissima extract, Bacillus fermentation product, Tilia europaea extract and ruberythrin is (1-5):(1-3):(1-2):(0.1-0.3), the barrier repair and anti-aging effects of the composition are better.

[0124] In summary, through the synergistic effect of the four components of Camellia nitidissima extract, Bacillus fermentation product, Tilia europaea extract and ruberythrin, the anti-aging composition of the present invention enhances the antioxidant and anti-aging effects of the composition, and by regulating the signal transmission between cells, improves the self-repair ability and barrier function of the skin, thereby achieving a synergistic anti-aging effect. It can also inhibit stress hormones, effectively improve the damage caused by negative emotions to the skin, repair the barrier, and enhance the overall health of the skin.

[0125] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit the protection scope of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. An anti-aging composition for promoting communication between skin cells, characterized in that, It comprises the following components: camellia nitidissima leaf extract, bacillus fermentation product, tilia europaea extract and rubrofusarin glucoside, and the weight ratio of the camellia nitidissima leaf extract, bacillus fermentation product, tilia europaea extract and rubrofusarin glucoside is (1-10):(1-8):(1-6):(0.1-1).

2. The anti-aging composition for promoting skin cell communication according to claim 1, characterized in that, The weight ratio of the camellia nitidissima leaf extract, bacillus fermentation product, tilia europaea extract and rubrofusarin glucoside is (1-8):(1-5):(1-4):(0.1-0.5).

3. The anti-aging composition for promoting skin cell communication according to claim 1, characterized in that, The weight ratio of the camellia nitidissima leaf extract, bacillus fermentation product, tilia europaea extract and rubrofusarin glucoside is (1-5):(1-3):(1-2):(0.1-0.3).

4. Use of the anti-aging composition for promoting skin cell communication according to any one of claims 1-3 in the preparation of cosmetics.

5. Use of the anti-aging composition for promoting skin cell communication according to claim 4 in the preparation of cosmetics, characterized in that, The cosmetics are lotion, emulsion, cream, facial mask, essence or spray, and the addition amount of the composition is 1%-3% of the total weight of the cosmetics.

6. An essence, characterized in that, It comprises raw materials in the following weight percentages: 1%-3% of the anti-aging composition according to any one of claims 1-3, 0.05%-0.5% of thickener, 3%-20% of humectant, 0.2%-5% of preservative, 0.05%-0.1% of pH regulator and 0.01%-1% of essence, and the balance is deionized water.

7. The essence according to claim 6, characterized in that, The raw materials are selected from at least one of (a)-(d): (a) The thickener includes at least one of xanthan gum, carbopol 980, AVC, and high molecular cellulose; (b) The humectant includes at least one of allantoin, sodium polyacrylate, hydrogenated lecithin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,2-butanediol, glycerol, pullulan and ceramide; (c) The pH regulator includes at least one of arginine, sodium hydroxide and EDTA-2Na; (d) The preservative includes at least one of 1,3-propanediol, 1,2-hexanediol and p-hydroxyacetophenone.

8. The preparation method of the essence according to claim 7, characterized in that, It comprises the following steps: S1. Mix the humectant, thickener and part of deionized water, homogenize at 75-85°C, then add the preservative and stir evenly to obtain a mixture; S2. Wait until the temperature of the mixture in S1 drops to 35-45°C, add each component of the anti-aging composition, essence and the remaining deionized water, stir evenly, and finally add the pH regulator to adjust the pH to obtain the essence.

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