Mild skin care composition for oily sensitive skin and preparation method thereof

Abstract

The invention relates to a mild skin care composition for oily sensitive skin and a preparation method thereof, and belongs to the technical field of skin care products. The skin care composition is prepared from the following components in percentage by mass: 60%-80% of bifidus yeast fermentation product filtrate, 15%-36% of galactose yeast-like bacterium fermentation product filtrate, 0.1%-1% of nicotinamide, 0.1%-1% of hydroxyethyl piperazine ethanesulfonic acid and 0.1%-1% of plant active ingredients, the mild skin care composition does not contain additional water, and the total mass percentage of the bifidus yeast fermentation product filtrate and the galactose yeast-like bacterium fermentation product filtrate is not less than 93%. The skin care composition has the effects of controlling oil, preserving moisture and repairing, has fresh texture and moisture-preserving and non-drying skin feeling, can meet the requirements of oil-sensitive skin people, does not additionally add industrial water, can keep high-activity component content, and is mild and efficient.

Description

Technical Field

[0001] The present invention belongs to the technical field of skin care products, and particularly relates to a mild skin care composition for oily sensitive skin and a preparation method thereof. Background Art

[0002] With the increase of environmental pollution and life pressure, more and more people are facing skin problems, such as oily skin, dry skin, and sensitive skin, etc. The skin characteristics of people with oily sensitive skin (oil-sensitive skin) are usually: strong oil secretion, and at the same time, the skin barrier function is weak, and it is easy to have an allergic reaction to external stimuli. Therefore, people with oil-sensitive skin have their particularity in the demand for skin care products, specifically reflected in: on the one hand, skin care products are required to control excessive oil secretion and reduce the occurrence of acne; on the other hand, skin care products are also required to provide sufficient moisture for the skin and improve the skin barrier function. In addition, people with oily skin usually pursue a fresh and non-greasy feeling in skin care products, and water-based cosmetics with a light texture are generally more suitable for the needs of this group of people. However, due to the limitations of water-based cosmetics themselves, in the current water-based products on the market, except for a small amount of added skin care ingredients, most of the components are added deionized water, and the improvement effect on the skin problems of people with oil-sensitive skin is average.

[0003] For example: Patent CN118402998A discloses a composition capable of repairing the barrier and anti-inflammation, and its preparation method and application. The disclosed composition includes 0.1%-5% of lysate of Saccharomyces boulardii fermentation product, 0.1%-1% of β-glucan, 0.1%-5% of galactose yeast fermentation broth, and the balance is phosphate buffer solution. Through the synergistic effect of the lysate of Saccharomyces boulardii fermentation product, β-glucan and galactose yeast fermentation broth, this composition achieves the effects of repairing the skin barrier and anti-inflammation. However, because its main component is phosphate buffer solution, this composition only plays the role of repairing the skin barrier and anti-inflammation, and does not achieve the oil control effect at the same time, and cannot solve the skin problems caused by strong skin oil secretion in people with oil-sensitive skin; and the content of its active ingredients is low, most of the composition is the balance of phosphate buffer solution, which will affect the moisturizing effect on the skin while being unable to control oil.

[0004] Therefore, it is an urgent technical problem to be solved at present to develop a skin care composition that combines oil control, moisturizing, and repair effects, and at the same time has a high content of active ingredients and a fresh texture to meet the needs of people with oil-sensitive skin. Summary of the Invention

[0005] In view of the above technical problems, the present invention provides a mild skin care composition for oily and sensitive skin and a preparation method thereof. The skin care composition has the functions of oil control, moisturization and repair, and has a fresh texture and a moisturizing and non-drying skin feel, which can meet the needs of people with oily and sensitive skin. At the same time, it does not add industrial water additionally, can maintain a high content of active ingredients, and the product is mild and highly effective.

[0006] The present invention provides a mild skin care composition for oily and sensitive skin, comprising the following components in mass percentage: 60% - 80% of filtrate of bifidobacterium longum fermentation product, 15% - 36% of filtrate of saccharomyces cerevisiae fermentation product, 0.1% - 1% of nicotinamide, 0.1% - 1% of N-(2-hydroxyethyl)piperazine-N'-ethanesulfonic acid, 0.1% - 1% of plant active ingredient; the mild skin care composition does not contain additionally added water, and the total mass percentage of the filtrate of bifidobacterium longum fermentation product and the filtrate of saccharomyces cerevisiae fermentation product is not less than 93%.

[0007] In some embodiments, in the mild skin care composition, the mass percentage of the filtrate of bifidobacterium longum fermentation product is 70%, the mass percentage of the filtrate of saccharomyces cerevisiae fermentation product is 25%, the mass percentage of nicotinamide is 0.5%, the mass percentage of N-(2-hydroxyethyl)piperazine-N'-ethanesulfonic acid is 0.5%, and the mass percentage of the plant active ingredient is 0.1%.

[0008] In some embodiments, the plant active ingredient is asiaticoside-II.

[0009] In some embodiments, the mild skin care composition further comprises the following components in mass percentage: 0.005% - 0.015% of essence, 0.025% - 0.075% of essence solubilizer.

[0010] In some embodiments, the essence is of grass and wood fragrance type, and the essence solubilizer is PEG-40 hydrogenated castor oil.

[0011] In some embodiments, the mild skin care composition further comprises polyol, and the mass percentage of the polyol in the mild skin care composition is not higher than 5%.

[0012] In some embodiments, the polyol is selected from at least one of butanediol, propylene glycol and glycerol.

[0013] In addition, the present invention also provides a preparation method of the mild skin care composition for oily and sensitive skin according to any one of the above technical solutions, comprising the following steps:

[0014] Prepare phase A: Mix the filtrate of bifidobacterium longum fermentation product, the filtrate of saccharomyces cerevisiae fermentation product, nicotinamide and N-(2-hydroxyethyl)piperazine-N'-ethanesulfonic acid to obtain phase A;

[0015] Adding plant active ingredients: Add the plant active ingredients to Phase A and mix evenly.

[0016] Discharging: Homogenize and then discharge after obtaining a clear and transparent solution.

[0017] In some of the embodiments, it further includes the step of adding fragrance. The specific operation of the adding fragrance step is: Mix the fragrance and the fragrance solubilizer, stir evenly and then add them to Phase A and mix evenly.

[0018] In some of the embodiments, it further includes the step of adding polyol. The specific operation of the adding polyol step is: Add the polyol to Phase A and mix evenly.

[0019] Compared with the prior art, the advantages and beneficial effects of the present invention are as follows:

[0020] 1. The mild skin care composition for oily and sensitive skin provided by the present invention combines two yeast fermentation product filtrates, namely the filtrate of Saccharomyces boulardii fermentation product and the filtrate of Saccharomycopsis ferment filtrate, with niacinamide, hydroxyethylpiperazineethanesulfonic acid and plant active ingredients to achieve synergistic effects: It can significantly inhibit the activity of 5α-reductase, thereby effectively regulating the activity of sebaceous gland cells and reducing sebum secretion, having a significant oil control effect and being able to improve the problem of excessive oil secretion in oily skin; it can significantly inhibit the activity of hyaluronidase, thereby effectively alleviating inflammation, having anti-inflammatory, anti-allergic and repair effects, and being able to improve the sensitive problem of oily skin; the total content of the filtrate of Saccharomyces boulardii fermentation product and the filtrate of Saccharomycopsis ferment filtrate is as high as 93% or more, which can efficiently moisturize, provide sufficient moisture for the skin, and improve the barrier function of oily skin; in short, it has the combined effects of oil control, moisturizing and repair, and has a broad application prospect in the field of skin care for people with oily and sensitive skin.

[0021] 2. In the mild skin care composition for oily and sensitive skin provided by the present invention, the content of the two yeast fermentation product filtrates, namely the filtrate of Saccharomyces boulardii fermentation product and the filtrate of Saccharomycopsis ferment filtrate, is increased to 93% or more, and the water in the filtrate raw material itself is used as the solvent without adding industrial water additionally. It not only maintains the light aqueous texture, obtains a moisturizing and non-drying skin feeling, provides a fresh and non-greasy use experience for people with oily and sensitive skin, but also increases the content of active ingredients, achieving efficient oil control, moisturizing and repair effects.

[0022] 3. The mild skin care composition for oily and sensitive skin provided by the present invention has a concise formulation system, avoiding potential irritation to the skin of people with oily and sensitive skin due to too many ingredients, achieving a mild and non-irritating use effect, and being friendly to people with oily and sensitive skin.

[0023] 4. The preparation method of the mild skin care composition for oily sensitive skin provided by the present invention does not require additional industrial water. The preparation process is simple, without complex equipment and cumbersome technological processes, which is both environmentally friendly and reduces production costs and energy consumption, facilitating large-scale industrial production and having good economic efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 In vitro 5α-reductase inhibition rates of the compositions prepared in Examples 1-4 and Comparative Examples 1-5 of the present invention, as well as raw materials YKDYJ-02 and BIO-BIFIDA FERMENT FILTRATE(Ⅲ);

[0025] Figure 2 In vitro hyaluronidase inhibition rates of the compositions prepared in Examples 1-4 and Comparative Examples 1-5 of the present invention, as well as raw materials YKDYJ-02 and BIO-BIFIDA FERMENT FILTRATE(Ⅲ);

[0026] Figure 3 Test results of the oil control efficacy of the composition prepared in Example 1 of the present invention on the human body;

[0027] Figure 4 VISA facial porphyrin test results of the composition prepared in Example 1 of the present invention, wherein (a) and (b) are the facial porphyrin characteristic visa images of Subject No. 1 before and 8 hours after use, respectively, and (c) and (d) are the facial porphyrin characteristic visa images of Subject No. 2 before and 8 hours after use, respectively. DETAILED DESCRIPTION OF THE INVENTION

[0028] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0029] The embodiments of the present invention provide a mild skin care composition for oily sensitive skin, comprising the following components in mass percentage: 60% - 80% of bifidobacterium ferment lysate, 15% - 36% of saccharomyces ferment filtrate, 0.1% - 1% of niacinamide, 0.1% - 1% of hydroxyethyl piperazine ethanesulfonic acid, and 0.1% - 1% of plant active ingredients; the mild skin care composition does not contain extra added water, and the total mass percentage of the bifidobacterium ferment lysate and the saccharomyces ferment filtrate is not less than 93%.

[0030] The above-mentioned mild skin care composition for oily and sensitive skin combines two yeast fermentation filtrates, namely the filtrate of Saccharomyces boulardii fermentation product and the filtrate of Saccharomyces cerevisiae fermentation product, with niacinamide, hydroxyethylpiperazineethanesulfonic acid and plant active ingredients to achieve synergistic effects: it can significantly inhibit the activity of 5α-reductase, thereby effectively regulating the activity of sebaceous gland cells and reducing sebum secretion, having a significant oil control effect and being able to improve the problem of excessive oil secretion in oily skin; it can significantly inhibit the activity of hyaluronidase, thereby effectively relieving inflammation, having anti-inflammatory, anti-allergic and repair effects, and being able to improve the sensitivity problem of oily skin; the total content of the filtrate of Saccharomyces boulardii fermentation product and the filtrate of Saccharomyces cerevisiae fermentation product is as high as 93% or more, which can efficiently moisturize, provide sufficient moisture for the skin, and improve the barrier function of oily skin; in short, it has the functions of oil control, moisturizing and repair, and has a broad application prospect in the field of skin care for people with oily and sensitive skin.

[0031] At the same time, in the above-mentioned mild skin care composition for oily and sensitive skin, the content of the two yeast fermentation filtrates, namely the filtrate of Saccharomyces boulardii fermentation product and the filtrate of Saccharomyces cerevisiae fermentation product, is increased to 93% or more, and the water in the filtrate raw material itself is used as a solvent without adding industrial water additionally. It not only maintains a light aqueous texture, obtains a moisturizing and non-drying skin feeling, provides a fresh and non-greasy use experience for people with oily and sensitive skin, but also increases the content of active ingredients, achieving efficient oil control, moisturizing and repair effects.

[0032] In addition, the above-mentioned mild skin care composition for oily and sensitive skin has a streamlined formula system, avoiding potential irritation to the skin of people with oily and sensitive skin due to too many ingredients, achieving a mild and non-irritating use effect, and being friendly to people with oily and sensitive skin.

[0033] In a preferred embodiment, in the mild skin care composition, the mass percentage of the filtrate of Saccharomyces boulardii fermentation product is 70%, the mass percentage of the filtrate of Saccharomyces cerevisiae fermentation product is 25%, the mass percentage of niacinamide is 0.5%, the mass percentage of hydroxyethylpiperazineethanesulfonic acid is 0.5%, and the mass percentage of plant active ingredients is 0.1%. This preferred embodiment further defines the optimal dosage of each component. The mild skin care composition obtained under this optimal dosage has better oil control, moisturizing and repair effects.

[0034] In a preferred embodiment, the plant active ingredient is asiaticoside-II. Using asiaticoside-II as the plant active ingredient, it has a soothing and anti-inflammatory effect, can reduce the irritation of the product, and improve the sensitivity problem of oily skin. It should be noted that the composition of asiaticoside-II specifically includes cyclodextrin and Centella asiatica extract, and its English trade name is Redumatte ASC-II.

[0035] In an alternative embodiment, the mild skin care composition further comprises the following components by mass percentage: essence 0.005% - 0.015%, essence solubilizer 0.025% - 0.075%. In this alternative embodiment, essence is further added to cover the smell of the fermented filtrate, which is beneficial to improving the consumer experience during use.

[0036] In a preferred embodiment, the essence is of a herbal fragrance type, and the essence solubilizer is PEG - 40 hydrogenated castor oil. In this preferred embodiment, the herbal fragrance type essence has a good smell covering effect and is more in line with the fresh smell perception of the oily sensitive skin population; using PEG - 40 hydrogenated castor oil as the essence solubilizer can improve the stability of the essence. It should be noted that the herbal fragrance type essence can specifically be rosemary (ROSMARINUS OFFICINALIS) leaf extract / lemon (CITRUS LIMON) fruit extract / Cupressus sempervirens oil / Cedrus deodara wood oil.

[0037] In an alternative embodiment, the mild skin care composition further comprises polyhydric alcohol, and the mass percentage of the polyhydric alcohol in the mild skin care composition is not higher than 5%. In this alternative embodiment, polyhydric alcohol is further added. It serves both as a humectant to further improve the product's moisturizing effect and as a solvent to fully disperse each component, improving the homogeneity and stability of the product. It should be noted that in this alternative embodiment, limiting the content of polyhydric alcohol to not higher than 5% can avoid the problem of the product being irritating due to excessive addition of polyhydric alcohol.

[0038] In a preferred embodiment, the polyhydric alcohol is selected from at least one of butanediol, propylene glycol, and glycerol. This preferred embodiment further lists the preferred types of polyhydric alcohols, which have good moisturizing effects and low irritation, and are more friendly to oily sensitive skin.

[0039] In an alternative embodiment, the mild skin care composition further comprises a preservative. The preservative can be selected from at least one of p - hydroxyacetophenone, 1,2 - hexanediol, 1,3 - propanediol, pentanediol, ethylhexyl glycerin ether, raspberry ketone, and octanoyl hydroxamic acid, which are conventional components of skin care products and can be used in conventional amounts.

[0040] The embodiments of the present invention also provide a preparation method for the above - mentioned mild skin care composition for oily sensitive skin, comprising the following steps:

[0041] Prepare Phase A: Mix the filtrate of bifidobacterium ferment lysate, the filtrate of saccharomycopsis ferment filtrate, niacinamide, and N - (2 - hydroxyethyl) piperazine - N'-ethanesulfonic acid to obtain Phase A;

[0042] Adding plant active ingredients: Add plant active ingredients to Phase A and mix evenly.

[0043] Discharging: Homogenize and then discharge after obtaining a clear and transparent solution.

[0044] The preparation method of the mild skin care composition for oily and sensitive skin does not require additional addition of industrial water. The preparation process is simple, without complex equipment and cumbersome technological processes, which is both environmentally friendly and reduces production costs and energy consumption, facilitating large-scale industrial production and having good economic efficiency.

[0045] In an alternative embodiment, the preparation method of the mild skin care composition for oily and sensitive skin further includes a step of adding fragrance. The specific operation of the step of adding fragrance is: Mix the fragrance and the fragrance solubilizer, stir evenly and then add it to Phase A and mix evenly. It should be noted that the step of adding fragrance is carried out after adding plant active ingredients and before discharging.

[0046] In an alternative embodiment, the preparation method of the mild skin care composition for oily and sensitive skin further includes a step of adding polyol. The specific operation of the step of adding polyol is: Add the polyol to Phase A and mix evenly. It should be noted that the step of adding polyol can be carried out before the step of adding fragrance, either before the step of adding plant active ingredients or after the step of adding plant active ingredients.

[0047] To introduce the mild skin care composition for oily and sensitive skin and its preparation method provided by the embodiments of the present invention more clearly and in detail, specific embodiments will be described below. It should be noted that in the following embodiments, the filtrate of the bifida ferment lysate is the commercial product BIO - BIFIDA FERMENT FILTRATE(Ⅲ), in which the mass percentage of the filtrate of the bifida ferment lysate is 99.1%, and this commercial product also contains 0.5% of 1,2 - hexanediol and 0.4% of p - hydroxyacetophenone; the filtrate of the saccharomyces galactomyces fermentation product is the commercial product YKDYJ - 02, in which the mass percentage of the filtrate of the saccharomyces galactomyces fermentation product is 98.3%, and this commercial product also contains 1% of 1,2 - hexanediol, 0.5% of p - hydroxyacetophenone and 0.2% of 1,2 - pentanediol. Since the commercial products BIO - BIFIDA FERMENT FILTRATE(Ⅲ) and YKDYJ - 02 already contain preservatives, no additional preservatives are added in each embodiment.

[0048] Example 1

[0049] A mild skin care composition for oily and sensitive skin, comprising the following components by mass percentage: 70% of filtrate of Saccharomyces boulardii fermentation product, 25% of filtrate of Saccharomycopsis ferment, 0.5% of niacinamide, 0.5% of 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid, 0.1% of asiaticoside-II, 0.01% of herbal fragrance essence (Rosmarinus officinalis leaf extract / Citrus limon fruit extract / Cupressus sempervirens oil / Cedrus atlantica oil), 0.05% of PEG-40 hydrogenated castor oil, and the balance being butanediol. For the specific dosage of each component, see Table 1 for details.

[0050] The preparation method of the above-mentioned mild skin care composition for oily and sensitive skin comprises the following steps:

[0051] Prepare Phase A: Mix the filtrate of Saccharomyces boulardii fermentation product, the filtrate of Saccharomycopsis ferment, niacinamide and 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid to obtain Phase A;

[0052] Add plant active ingredients: Add asiaticoside-II to Phase A and mix evenly to obtain mixture a;

[0053] Add polyol: Add butanediol to mixture a and mix evenly to obtain mixture b;

[0054] Add essence: Mix the herbal fragrance essence and PEG-40 hydrogenated castor oil, stir evenly and then add it to mixture b and mix evenly;

[0055] Discharge: Homogenize and discharge after obtaining a clear and transparent solution.

[0056] Example 2

[0057] The difference between this example and Example 1 lies in that the dosages of each component are different. For the specific dosage of each component, see Table 1 for details.

[0058] Example 3

[0059] The difference between this example and Example 1 lies in that the dosages of each component are different. For the specific dosage of each component, see Table 1 for details.

[0060] Example 4

[0061] The difference between this example and Example 1 lies in that the dosages of each component are different. For the specific dosage of each component, see Table 1 for details.

[0062] Comparative Example 1

[0063] The difference between this example and Example 1 lies in that only the filtrate of Saccharomyces boulardii fermentation product is used without adding the filtrate of Saccharomycopsis ferment while keeping the total mass percentage of the yeast fermentation product filtrate unchanged. For the specific dosage of each component, see Table 1 for details.

[0064] Comparative Example 2

[0065] The difference between this example and Example 1 is that, while keeping the total mass percentage of the yeast fermentation product filtrate unchanged, only the fermentation product filtrate of Saccharomyces galactomyces is used, and the fermentation product filtrate of Saccharomyces boulardii is not added. For the specific dosage of each component, please refer to Table 1.

[0066] Comparative Example 3

[0067] The difference between this example and Example 1 is that niacinamide and hydroxyethylpiperazineethanesulfonic acid are not added. For the specific dosage of each component, please refer to Table 1.

[0068] Comparative Example 4

[0069] The difference between this example and Example 1 is that niacinamide, hydroxyethylpiperazineethanesulfonic acid and asiaticoside-II are not added. For the specific dosage of each component, please refer to Table 1.

[0070] Table 1 Dosage of each component in Examples 1-4 and Comparative Examples 1-4 (mass percentage / %)

[0071]

[0072] Performance test

[0073] 1. In vitro 5α-reductase inhibition experiment

[0074] The 5α-reductase activity inhibition degrees of the compositions prepared in Examples 1-4, Comparative Examples 1-5, and the raw materials YKDYJ-02 and BIO-BIFIDA FERMENTFILTRATE(Ⅲ) were respectively detected to evaluate the oil control efficacy.

[0075] The experimental groups are as follows:

[0076] Blank control group: phosphate buffer solution;

[0077] Positive control group: finasteride aqueous solution with a concentration of 0.5 mg / ml;

[0078] The test groups include: Example 1 group (the composition prepared in Example 1), Example 2 group (the composition prepared in Example 2), Example 3 group (the composition prepared in Example 3), Example 4 group (the composition prepared in Example 4), Comparative Example 1 group (the composition prepared in Comparative Example 1), Comparative Example 2 group (the composition prepared in Comparative Example 2), Comparative Example 3 group (the composition prepared in Comparative Example 3), Comparative Example 4 group (the composition prepared in Comparative Example 4), 2% YKDYJ-02 group (YKDYJ-02 was diluted 50 times with water), 5% YKDYJ-02 group (YKDYJ-02 was diluted 20 times with water), 90% YKDYJ-02 group (YKDYJ-02 and water were mixed in a volume ratio of 9:1), and 90% BIO-BIFIDA FERMENT FILTRATE group (BIO-BIFIDA FERMENT FILTRATE and water were mixed in a volume ratio of 9:1).

[0079] According to the instructions of the human 5α-reductase ELISA kit (abmart AB-15958B), a multifunctional microplate reader (PerkinElmer (Shanghai) EnSightTM HH3400NMDTW-E008) was used to detect the 5α-reductase activity inhibition rate. The specific steps are as follows:

[0080] (1) Take out the required strips from the aluminum foil bag after equilibration at room temperature for 20 minutes;

[0081] (2) Sample addition: Each experimental group uses three parallel wells. First, add 25 μL of 5α-reductase standard with a concentration of 20 U / mL to each well, and then add the sample of each experimental group with a sample volume of 25 μL. Incubate in a 37°C thermostatic reactor for 60 min.

[0082] (3) Discard the liquid, pat dry with absorbent paper, fill each well with washing solution, let stand for 1 min, shake off the washing solution, pat dry with absorbent paper, and repeat this process 5 times;

[0083] (4) Add 100 μL of horseradish peroxidase (HRP)-labeled detection antibody to each well, seal the well with a sealing film, and incubate in a 37°C thermostat for 60 min;

[0084] (5) Discard the liquid, pat dry with absorbent paper, fill each well with washing solution, let stand for 1 min, shake off the washing solution, pat dry with absorbent paper, and repeat this process 5 times;

[0085] (6) Add 100 μL of the mixture of substrates A and B to each well and incubate at 37°C in the dark for 15 min;

[0086] (7) Add 50 μL of stop solution to each well. Measure the OD value of each well at a wavelength of 450 nm within 15 min, and then calculate the 5α-reductase inhibition rate of each experimental group sample.

[0087] The test results are as Figure 1 shown. It can be seen that the inhibition rate of 5α-reductase activity of the compositions prepared in Examples 1-4 reaches more than 73%, which is significantly higher than that of Comparative Examples 1-4 and the raw materials YKDYJ-02 and BIO-BIFIDA FERMENT FILTRATE(Ⅲ). Thus, in the mild skin care composition for oily and sensitive skin provided by the embodiments of the present invention, the bifida ferment lysate, saccharomyces ferment filtrate, niacinamide, hydroxyethylpiperazine ethanesulfonic acid and plant active ingredients play a synergistic effect, can significantly inhibit the activity of 5α-reductase, thereby effectively regulating the activity of sebaceous gland cells and reducing sebum secretion, has a significant oil control effect, and can improve the problem of excessive oil secretion in oily skin.

[0088] 2. In vitro hyaluronidase inhibition experiment

[0089] Hyaluronidase is a hydrolase that degrades hyaluronic acid and is the main component of the extracellular matrix of connective tissue cells in the body. It is related to most type I allergic reactions mediated by IgE and type IV allergic reactions mediated by T cells. Therefore, the in vitro hyaluronidase inhibition experiment can, to a certain extent, reflect the soothing, anti-allergic and anti-inflammatory effects of the corresponding products on the skin. Hyaluronic acid is a high-molecular polysaccharide composed of D-glucuronic acid and N-acetylglucosamine, and can carry out a color reaction according to the Elson-morgan reaction. Through this reaction, the inhibition rate of hyaluronidase in the sample can be measured.

[0090] The in vitro hyaluronidase inhibition experiments were respectively carried out on the compositions prepared in Examples 1-4, Comparative Examples 1-5 and the raw materials YKDYJ-02 and BIO-BIFIDA FERMENT FILTRATE(Ⅲ) to evaluate the anti-allergic and anti-inflammatory effects.

[0091] The experimental groups are as follows:

[0092] Negative control group: Prepared according to the test method requirements of the "Verification and Detection Rules for the Soothing Efficacy of Cosmetics";

[0093] The test groups include: Example 1 group (the composition prepared in Example 1 is diluted 20 times with water), Example 2 group (the composition prepared in Example 2 is diluted 20 times with water), Example 3 group (the composition prepared in Example 3 is diluted 20 times with water), Example 4 group (the composition prepared in Example 4 is diluted 20 times with water), Comparative Example 1 group (the composition prepared in Comparative Example 1 is diluted 20 times with water), Comparative Example 2 group (the composition prepared in Comparative Example 2 is diluted 20 times with water), Comparative Example 3 group (the composition prepared in Comparative Example 3 is diluted 20 times with water), Comparative Example 4 group (the composition prepared in Comparative Example 4 is diluted 20 times with water), YKDYJ-02 group (YKDYJ-02 is diluted 20 times with water), BIO-BIFIDA FERMENT FILTRATE group (BIO-BIFIDA FERMENT FILTRATE is diluted 20 times with water).

[0094] In vitro hyaluronidase inhibition rate detection method: According to the laboratory method (enzyme inhibition rate method) of QTTZ-XZ-35 "Verification and Detection Rules for the Soothing Efficacy of Cosmetics", the inhibition rate of hyaluronidase in the samples of each test group was determined, and by comparing with the negative control group, it was determined whether the samples of each test group had soothing efficacy.

[0095] The experimental results are as Figure 2 shown. It can be seen that the hyaluronidase inhibition rate of the compositions prepared in Examples 1-4 of the present invention reaches more than 40%, which is significantly higher than that of Comparative Examples 1-4 and the raw materials YKDYJ-02 and BIO-BIFIDA FERMENT FILTRATE (Ⅲ). Thus, in the mild skin care composition for oily sensitive skin provided by the embodiments of the present invention, bifidobacterium ferment lysate, saccharomyces ferment filtrate, niacinamide, hydroxyethylpiperazineethanesulfonic acid and plant active ingredients play a synergistic effect, can significantly inhibit the activity of hyaluronidase, thereby effectively alleviating inflammation, having anti-inflammatory, anti-allergic and repair effects, and can improve the sensitive problem of oily skin.

[0096] 3. Human sebum control efficacy test

[0097] The Sebumeter test method was used to test the sebum control effect of the composition prepared in Example 1. The test principle of the Sebumeter test method is: based on the working principle of a spectrophotometer, a tape is used to absorb the oil on the human skin, and the oil will change the light transmittance of the tape. The more oil is absorbed, the greater the light transmittance will be. Therefore, the skin oil secretion amount can be measured by the light transmittance.

[0098] The steps of the human sebum control efficacy test are as follows:

[0099] (1) Select test subjects: In a thermostatic and humidistatic chamber (temperature 21 ± 1°C, relative humidity 50 ± 10%), first use the Sebumeter test method to measure the original value of the facial skin sebum secretion of the candidate volunteers (25 - 35 years old, female). Select 10 volunteers with similar facial skin sebum secretion before application for the application experiment.

[0100] (2) Clean the volunteers' faces with the reference facial cleanser. After drying, use the Sebumeter test method to measure the initial value of skin sebum secretion; apply 0.5 g of the composition prepared in Example 1 (experimental group) on half of the volunteers' faces, and do not use any product on the other half (blank group). Apply evenly and measure the skin sebum secretion at 10 min and 4 h after application.

[0101] The test results are as Figure 3 shown. It can be seen that within 10 min, the skin sebum secretion of both the blank group (without using any product) and the experimental group (using 0.5 g of the composition prepared in Example 1) decreased significantly, and the difference between the two was small; after 4 h, the skin sebum secretion of the blank group increased significantly and was close to the initial value, indicating that the blank group had no obvious oil control effect. While the skin sebum secretion of the experimental group increased, it was still 35.6% less than the initial value, indicating that the mild skin care composition for oily and sensitive skin provided in the embodiment of the present invention has an obvious oil control effect.

[0102] 4. VISA Facial Porphyrin Test

[0103] The VISA facial porphyrin test is a technique for visualizing the porphyrins (Porphyrins) in the skin through a professional skin detection device (such as a VISIA skin analyzer). Porphyrin is a metabolite of Propionibacterium acnes and will show a fluorescence reaction (usually orange - red or white bright spots) under ultraviolet light (UV). By comparing the number of porphyrins before and after treatment, the effectiveness of the oil control effect of skin care products can be evaluated.

[0104] Detect the skin porphyrin conditions of 10 subjects at 0 h and 8 h after using the composition prepared in Example 1 through a professional skin detector, the Visa skin detector. Statistic the data to obtain the skin porphyrin characteristic count. The VISA facial porphyrin test method is as follows: After the subjects treat their facial skin according to the experimental requirements, under the guidance of the Visa operator, gently place the head in the designated area of the instrument, keep still, and then the operator takes a photo for collection. And through the professional software supporting the instrument, perform image analysis on the collected facial photos of the subjects and record the porphyrin characteristic count. Among them, the visa images of the facial porphyrin characteristics of 2 subjects are as Figure 4As shown, the counted results of skin porphyrin characteristics obtained statistically are shown in Table 2.

[0105] Table 2 Counted Results of Skin Porphyrin Characteristics of 2 Subjects

[0106] Subject number Before use 8 h after use 1 6679 5641 2 4064 2954

[0107] It can be seen that after the subjects used the composition prepared in Example 1, the count of facial porphyrin characteristics decreased significantly within 8 hours. Thus, it shows that the mild skin care composition for oily sensitive skin provided by the embodiments of the present invention has an obvious oil control effect.

Claims

1. A mild skin care composition for oily and sensitive skin, characterized in that, It comprises the following components by mass percentage: 60% - 80% of bifida ferment lysate filtrate, 15% - 36% of saccharomyces ferment filtrate, 0.1% - 1% of niacinamide, 0.1% - 1% of hydroxyethylpiperazine ethanesulfonic acid, and 0.1% - 1% of plant active ingredient; the mild skin care composition does not contain extra added water, and the total mass percentage of the bifida ferment lysate filtrate and the saccharomyces ferment filtrate is not less than 93%.

2. The mild skin care composition for oily and sensitive skin according to claim 1, characterized in that, In the mild skin care composition, the mass percentage of the bifida ferment lysate filtrate is 70%, the mass percentage of the saccharomyces ferment filtrate is 25%, the mass percentage of niacinamide is 0.5%, the mass percentage of hydroxyethylpiperazine ethanesulfonic acid is 0.5%, and the mass percentage of the plant active ingredient is 0.1%.

3. The mild skin care composition for oily and sensitive skin according to claim 1 or 2, characterized in that, The plant active ingredient is asiaticoside-II.

4. The mild skin care composition for oily and sensitive skin according to claim 1 or 2, characterized in that, The mild skin care composition further comprises the following components by mass percentage: 0.005% - 0.015% of essence, and 0.025% - 0.075% of essence solubilizer.

5. The mild skin care composition for oily and sensitive skin according to claim 4, characterized in that, The essence is of herbal fragrance type, and the essence solubilizer is PEG - 40 hydrogenated castor oil.

6. The mild skin care composition for oily and sensitive skin according to claim 1 or 2, characterized in that, The mild skin care composition further comprises polyol, and the mass percentage of the polyol in the mild skin care composition is not higher than 5%.

7. The mild skin care composition for oily and sensitive skin according to claim 6, characterized in that, The polyol is selected from at least one of butanediol, propylene glycol and glycerol.

8. A method for preparing the mild skin care composition for oily sensitive skin according to any one of claims 1-7, characterized in that, It includes the following steps: Prepare phase A: mix bifida ferment lysate filtrate, saccharomyces ferment filtrate, niacinamide and hydroxyethylpiperazine ethanesulfonic acid to obtain phase A; Add plant active ingredient: add the plant active ingredient to phase A and mix evenly; Discharge: homogenize, and discharge after obtaining a clear and transparent solution.

9. The preparation method of the mild skin care composition for oily sensitive skin according to claim 8, characterized in that, It further includes the step of adding essence, and the step of adding essence is specifically: mix the essence and the essence solubilizer, stir evenly and then add it to phase A and mix evenly.

10. The preparation method of the mild skin care composition for oily sensitive skin according to claim 8, characterized in that, It further includes the step of adding polyol, and the step of adding polyol is specifically: add the polyol to phase A and mix evenly.

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