Deuterium-depleted ganoderma water, traditional Chinese medicine beverage for treating kidney-yang deficiency and preparation method of deuterium-depleted ganoderma water

Abstract

The invention discloses deuterium-depleted ganoderma water, a traditional Chinese medicine beverage for treating kidney-yang deficiency and a preparation method of the deuterium-depleted ganoderma water. The deuterium-depleted ganoderma water is prepared from the following raw materials in parts by weight: 150-800 parts of deuterium-depleted water, 50-100 parts of ganoderma and 0.5-10 parts of an additive, the additives comprise niobic acid, phosphotungstic acid and sodium pyrophosphate. Niobic acid, phosphotungstic acid and sodium pyrophosphate have a synergistic effect, the niobic acid softens plant cell walls firstly, the porous structure of the niobic acid adsorbs cell wall fragments, and the physical barrier of subsequent reaction is reduced; the phosphotungstic acid accurately attacks a weak area after niobic acid action, hemicellulose and lignin cross-linking bonds are oxidized and broken, and the rigidity of cell walls is further reduced; the sodium pyrophosphate is combined with the effects of chelating metal ions and weakening the cross-linking degree of cell walls to jointly promote dissolution of effective components in the ganoderma lucidum; niobic acid, phosphotungstic acid and sodium pyrophosphate have a synergistic effect, and the leaching rate of active ingredients in ganoderma lucidum can be effectively increased.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicine, and particularly relates to a low-deuterium ganoderma water, a traditional Chinese medicine beverage for treating kidney-yang deficiency, and a preparation method thereof. Background Art

[0002] In recent years, with the accelerating social rhythm, people are generally in a sub-healthy state, and frequently show symptoms such as reduced vitality, decreased function and adaptability within a certain period of time. Traditional Chinese medicine believes that "the congenital foundation of a person lies in the kidney, and the kidney-yang dominates the yang qi of the whole body. When the fire declines, the symptoms of yang deficiency will occur repeatedly", and the clinical manifestations of kidney-yang deficiency coincide extremely with those of sub-health, such as: fatigue, listlessness, low vitality, easy fatigue, etc. The health of the kidneys is particularly important for normal life. The demand for health care products for treating kidney-yang deficiency is getting higher and higher. At present, the main treatment methods for kidney-yang deficiency in the market are drug therapies (including traditional Chinese medicine and western medicine), but the side effects of drug therapies are relatively large and are not suitable for the symptoms of kidney-yang deficiency that require long-term conditioning. Therefore, the development of drugs with no side effects for warming the kidney and subduing yang and strengthening the body has become a research hotspot. Summary of the Invention

[0003] To overcome the above-mentioned drawbacks, the purpose of the present invention is to provide a low-deuterium ganoderma water, a traditional Chinese medicine beverage for treating kidney-yang deficiency, and a preparation method thereof, so as to prepare a traditional Chinese medicine beverage with no side effects for warming the kidney and subduing yang and strengthening the body.

[0004] To achieve the above purpose, the technical solution adopted by the present invention is: a low-deuterium ganoderma water, calculated by weight, the preparation raw materials of the low-deuterium ganoderma water include 150-800 parts of low-deuterium water, 50-100 parts of ganoderma, and 0.5-10 parts of additives, wherein the additives include niobic acid, phosphotungstic acid and sodium pyrophosphate.

[0005] Among them, the low-deuterium water is natural small-molecule low-deuterium glacier hot spring water, D / (D + H) is about 1:6600, and the deuterium content is less than 150 PPM; it is from the low-deuterium ice spring in Bulong Grand Canyon, Nilka County, Xinjiang. The small-molecule cluster of the low-deuterium water has a high permeability; and the water rich in deuterium has the effect of an antioxidant, promoting the proliferation of normal cells.

[0006] Ganoderma, also known as the meat ganoderma, its components have anti-cancer effects and are not likely to cause mitochondrial blockage or redox imbalance, thereby inhibiting the growth of tumor cells.

[0007] Further, the mass ratio of ganoderma to low-deuterium water is 1:(3-8).

[0008] The inventor found during the research and development process that by adding additives externally, the leaching rate of the active ingredients in ganoderma can be improved. The reason may be that:

[0009] Niobium acid: In the process of extracting the active ingredients of Tai Sui, niobium acid, as an acid catalyst, may help to destroy the plant cell wall and promote the dissolution of the active ingredients. In addition, niobium acid also has a large specific surface area and adsorption capacity, which can adsorb impurities such as proteins and polysaccharides in the extract, thereby improving the purity of the extract.

[0010] Phosphotungstic acid: It has strong oxidizing properties and may be able to break polysaccharide chains through oxidation to destroy the cross-linked structure of plant cell walls, thereby helping to improve the extraction efficiency of active ingredients. In addition, while phosphotungstic acid oxidizes and decomposes fat-soluble impurities, its heteropolyanions form reversible inclusion complexes with alkaloid components to inhibit the dissolution of ineffective components.

[0011] Sodium pyrophosphate: chelates Ca in the cell wall of medicinal materials 2+ Mg 2+ Plasma reduces the mechanical strength of cell walls and promotes the dissolution of active ingredients.

[0012] Further, in parts by weight, the additive includes 0.4-3.5 parts of niobic acid, 0.1-2.5 parts of phosphotungstic acid, and 0.1-0.5 parts of sodium pyrophosphate. Exemplarily, niobic acid is in the range of 0.4 parts, 0.5 parts, 1.0 parts, 1.5 parts, 2.0 parts, 2.5 parts, 3.0 parts, 3.5 parts, or any two of them; phosphotungstic acid is in the range of 0.1 parts, 0.5 parts, 1.0 parts, 1.5 parts, 2.0 parts, 2.5 parts, or any two of them; sodium pyrophosphate is in the range of 0.1 parts, 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts, or any two of them.

[0013] Furthermore, the amount of the additive does not exceed 1% of the total mass of the ginseng and the deuterium-depleted water.

[0014] Furthermore, the concentration of niobic acid is 0.1-0.6% (the percentage of niobic acid used in the total mass of deuterium-depleted water).

[0015] Preferably, the concentration of niobic acid is 0.25-0.45%.

[0016] Furthermore, the concentration of phosphotungstic acid is 0.01-0.4% (the percentage of phosphotungstic acid used in the total mass of deuterium-depleted water).

[0017] Preferably, the concentration of phosphotungstic acid is 0.08-0.3%.

[0018] Furthermore, the concentration of sodium pyrophosphate is 0.01-0.12% (the percentage of sodium pyrophosphate used in the total mass of deuterium-depleted water).

[0019] Preferably, the concentration of sodium pyrophosphate is 0.05-0.1%.

[0020] Furthermore, the raw materials for preparing the low-deuterium Taizui water also include 4-53 parts of activated carbon.

[0021] Activated carbon can preferentially capture free heavy metals (such as Nb 5+ , W 6+ , etc.) through the dual action mechanisms of physical adsorption (van der Waals force) and chemical adsorption (coordination of surface oxygen-containing functional groups).

[0022] Furthermore, the mass ratio of activated carbon to the total amount of niobic acid and phosphotungstic acid is (8-10):1.

[0023] A method for preparing low-deuterium Taizui water includes the following steps:

[0024] S1. Dissolve the additives in low-deuterium water, then soak Taizui in the low-deuterium water, place it in a dark environment, stir evenly, soak, and perform rough filtration to obtain a low-deuterium Taizui crude liquid;

[0025] S2. Add activated carbon to the low-deuterium Taizui crude liquid, stir evenly, and perform rough filtration and fine filtration to obtain low-deuterium Taizui water.

[0026] Furthermore, in step S1, first add niobic acid and phosphotungstic acid in the additives to the low-deuterium water, and after dissolution, then add sodium pyrophosphate for dissolution.

[0027] Furthermore, in step S1, the soaking time is 5-10 hours.

[0028] Furthermore, in step S1 and / or step S2, the rough filtration is specifically carried out by filtering through a filter cloth with a mesh size of 200-400 meshes.

[0029] Furthermore, in step S2, the stirring temperature is 25-35 °C and the stirring time is 30-60 minutes.

[0030] Furthermore, in step S2, the fine filtration is specifically carried out by filtering through a microporous membrane with a pore size of 0.2-0.6 μm.

[0031] A traditional Chinese medicine beverage for treating kidney yang deficiency includes the above-mentioned low-deuterium Taizui water and traditional Chinese medicine powder. The traditional Chinese medicine powder includes 0.5-3 parts of dendrobium powder, 2-8 parts of wolfberry powder, 0.5-3 parts of ginseng powder, 1-9 parts of astragalus powder, 1-7 parts of cornel powder, 1-10 parts of roasted licorice powder, 1-9 parts of polygonatum powder, and 1-6 parts of cistanche powder.

[0032] This traditional Chinese medicine beverage has the effects of tonifying kidney yang, nourishing essence and blood, nourishing the liver and kidney, and improving eyesight with essence. It has a good conditioning effect on symptoms such as kidney yang weakness, essence qi deficiency and cold, and liver and kidney deficiency.

[0033] Low-deuterium Tai Sui water is a good solvent, and most of the effective ingredients in Chinese medicinal materials can be dissolved in it, which can improve the stability of the drug effect; the high permeability characteristics of low-deuterium water small molecular clusters can also fully exert the efficacy of the drug; the present invention uses low-deuterium Tai Sui water as a matrix, and soaks Chinese medicinal materials in it to precipitate effective ingredients, thereby achieving the effects of warming and nourishing kidney yang, nourishing yin and blood, and prolonging life and beautifying the skin, and exerts good health care and recuperation value without side effects.

[0034] Moreover, this formula not only utilizes the characteristics of low-deuterium water, but also combines the efficacy of traditional Chinese medicinal materials such as dendrobium, wolfberry, and ginseng, achieving comprehensive conditioning of kidney yang deficiency symptoms. At the same time, the functional complementarity and synergy between the Chinese medicine in the present invention and low-deuterium Tai Sui water not only improve the absorption efficiency of the drug, but also reduce the side effects, achieving the comprehensive effects of clearing the kidney, protecting the kidney, conditioning the body, and regulating immunity. In addition, the formula also particularly emphasizes the harmony of yin and yang, avoids excessive nourishment, helps prevent the occurrence of hypertension, hyperlipidemia and other diseases, and embodies the holistic and balanced view of traditional Chinese medicine treatment.

[0035] Preferably, the Chinese medicine powder includes 1-2 parts of dendrobium powder, 3-6 parts of wolfberry powder, 1-2 parts of ginseng powder, 2-6 parts of astragalus powder, 2-4 parts of cornus powder, 3-8 parts of licorice powder, 3-7 parts of polygonatum powder, and 2-4 parts of cistanche powder.

[0036] A preparation method of a Chinese medicinal beverage for treating kidney-yang deficiency comprises the following steps:

[0037] Step 1, weighing the Chinese medicine raw material powders of each component, mixing them evenly to obtain Chinese medicine powder;

[0038] Step 2, placing the Chinese medicine powder in low-deuterium water, stirring evenly, and standing to obtain expanded Chinese medicine; placing the expanded Chinese medicine into a percolation cylinder, flattening it, and covering it with filter paper;

[0039] Step 3, add low-deuterium water to the percolation cylinder, open the percolation liquid outlet, collect the outflowing percolation liquid and pour it into the percolation cylinder, and add low-deuterium water until the low-deuterium water submerges the swollen Chinese medicine and fully impregnates it;

[0040] Step 4: percolating and collecting the percolation liquid to obtain the Chinese medicinal beverage.

[0041] Furthermore, in step 1, the particle size of the Chinese medicine powder is 75-250 μm; if the raw material particles are too fine, they are prone to clogging and the leaching effect is poor; if they are too coarse, they are difficult to compact and the contact area between the solvent and the medicinal material is small.

[0042] Furthermore, in step 2, the mass of the low-deuterium Tai Sui water used is 1-3 times the mass of the traditional Chinese medicine powder.

[0043] Furthermore, in step 2, the standing time is 5-10 hours.

[0044] The traditional Chinese medicine beverage adopts percolation as the preparation method, which can more effectively extract the active ingredients of traditional Chinese medicinal materials from low-deuterium Taisui water. Avoiding the use of traditional Chinese medicine extracts, decocting and other processes, solving the problems of complex processes and low bioavailability commonly existing in existing traditional Chinese medicines, this preparation technology improves the extraction efficiency and purity of drug ingredients, thus enhancing the medicinal effect, obtaining a health care beverage with instant drinkability, no side effects, and improved bioavailability of active ingredients of traditional Chinese medicine, with stable medicinal effect and obvious improvement effect after taking. The raw materials used have good biocompatibility, without adding other chemicals additionally, reducing resource consumption and environmental pollution in the preparation process.

[0045] Further, in step 3, the height of the low-deuterium water submerging and swelling the upper surface of the traditional Chinese medicine is 6-10 cm, and the impregnation time is 20-30 h.

[0046] Further, in step 4, the percolation flow rate is 1-3 ml / min.

[0047] Further, in step 4, the collected percolate is disinfected and packaged to obtain the traditional Chinese medicine beverage.

[0048] Further, in step 4, the total addition amount of the swollen traditional Chinese medicine does not exceed 2 / 3 of the volume of the percolation cylinder.

[0049] Beneficial effects:

[0050] 1) During the leaching process of the active ingredients of low-deuterium Taisui, niobic acid, phosphotungstic acid, and sodium pyrophosphate play a synergistic role. Niobic acid first softens the plant cell wall, and its porous structure adsorbs cell wall fragments, reducing the physical barrier for subsequent reactions; phosphotungstic acid then precisely attacks the weak areas after the action of niobic acid, oxidatively breaking the cross-linking bonds of hemicellulose and lignin, further reducing the rigidity of the cell wall; combined with the role of sodium pyrophosphate in chelating metal ions and weakening the cross-linking degree of the cell wall, they jointly promote the dissolution of the active ingredients in Taisui; niobic acid, phosphotungstic acid, and sodium pyrophosphate play a synergistic role, which can effectively improve the leaching rate of the active ingredients in Taisui. At the same time, the antioxidant property of sodium pyrophosphate and the thermal stability of phosphotungstic acid can jointly protect heat-sensitive components such as glycosides, reducing losses during the extraction process.

[0051] 2) The present invention combines low-deuterium Taisui water with traditional Chinese medicine to form a new traditional Chinese medicine formula. This formula not only utilizes the characteristics of low-deuterium water but also combines the medicinal effects of traditional Chinese medicinal materials such as Dendrobium officinale, Lycium barbarum, and Panax ginseng, achieving comprehensive conditioning of kidney yang deficiency symptoms. At the same time, the functional complementarity and synergistic effect between the traditional Chinese medicine and low-deuterium Taisui water in the present invention, through this synergistic effect, not only improve the absorption efficiency of the drug but also reduce side effects, achieving the comprehensive effects of clearing and protecting the kidneys, regulating the body, and adjusting immunity. Specific embodiments

[0052] To make the above objects, features, and advantages of the present invention more apparent and understandable, the following provides a detailed description of the specific embodiments of the present invention. Many specific details are set forth in the following description to facilitate a full understanding of the present invention. However, the present invention can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without departing from the connotation of the present invention. Therefore, the present invention is not limited by the specific embodiments disclosed below.

[0053] Unless otherwise indicated in the operating examples or otherwise stated, all numbers used in the specification and claims to represent the amounts of ingredients, physical and chemical properties, etc. are understood to be adjusted by the term "about" in all cases. Therefore, unless otherwise stated, the numerical parameters listed in the above specification and appended claims are approximate values, and those skilled in the art can appropriately change these approximate values in order to obtain the desired characteristics by using the teachings disclosed herein. The use of numerical ranges expressed by endpoints includes all numbers within that range and any range within that range. For example, the range from 1 to 5 includes 1, 1.1, 1.3, 1.5, 2, 2.75, 3, 3.80, 4, and 5, and so on.

[0054] A traditional Chinese medicine beverage for treating kidney yang deficiency, comprising low-deuterium taizi water and traditional Chinese medicine powder. The traditional Chinese medicine powder includes 0.5 - 3 parts of dendrobium powder, 2 - 8 parts of wolfberry powder, 0.5 - 3 parts of ginseng powder, 1 - 9 parts of astragalus powder, 1 - 7 parts of cornel powder, 1 - 10 parts of roasted licorice powder, 1 - 9 parts of polygonatum powder, and 1 - 6 parts of cistanche powder.

[0055] In some embodiments, the traditional Chinese medicine powder includes 1 - 2 parts of dendrobium powder, 3 - 6 parts of wolfberry powder, 1 - 2 parts of ginseng powder, 2 - 6 parts of astragalus powder, 2 - 4 parts of cornel powder, 3 - 8 parts of roasted licorice powder, 3 - 7 parts of polygonatum powder, and 2 - 4 parts of cistanche powder.

[0056] For the low-deuterium taizi water used in the above traditional Chinese medicine beverage, by weight, the raw materials for preparing the low-deuterium taizi water include 150 - 800 parts of low-deuterium water, 50 - 100 parts of taizi, and 0.5 - 10 parts of additives.

[0057] In some embodiments, the mass ratio of taizi to low-deuterium water is 1:3 - 8.

[0058] In some embodiments, the dosage of the additive does not exceed 1% of the total mass of taizi and low-deuterium water.

[0059] In some embodiments, by weight, the additives include 0.4 - 3.5 parts of niobic acid, 0.1 - 2.5 parts of phosphotungstic acid, and 0.1 - 0.5 parts of sodium pyrophosphate.

[0060] In some embodiments, the concentration of niobic acid is 0.1-0.6% (the percentage of the amount of niobic acid in the total mass of low-deuterium water).

[0061] In some embodiments, the concentration of niobic acid is 0.25-0.45%.

[0062] In some embodiments, the concentration of phosphotungstic acid is 0.01-0.4% (the percentage of the amount of phosphotungstic acid in the total mass of low-deuterium water).

[0063] In some embodiments, the concentration of phosphotungstic acid is 0.08-0.3%.

[0064] In some embodiments, the concentration of sodium pyrophosphate is 0.01-0.12% (the percentage of the amount of sodium pyrophosphate in the total mass of low-deuterium water).

[0065] In some embodiments, the concentration of sodium pyrophosphate is 0.05-0.1%.

[0066] In some embodiments, the raw materials for preparing the low-deuterium Taizishen water further include 4-53 parts of activated carbon.

[0067] In some embodiments, the mass ratio of the activated carbon to the total amount of niobic acid and phosphotungstic acid is (8-10):1.

[0068] The method for preparing the above-mentioned low-deuterium Taizishen water includes the following steps:

[0069] S1. Dissolve the additives in the low-deuterium water, then immerse the Taizishen in the low-deuterium water, place it in a dark environment, stir evenly, soak, and obtain a crude low-deuterium Taizishen liquid through coarse filtration.

[0070] S2. Add activated carbon to the crude low-deuterium Taizishen liquid, stir evenly, and obtain the low-deuterium Taizishen water through coarse filtration and fine filtration.

[0071] In some embodiments, in step S1, first add niobic acid and phosphotungstic acid in the additives to the low-deuterium water, dissolve them, and then add sodium pyrophosphate for dissolution.

[0072] In some embodiments, in step S1, the soaking time is 5-10 hours.

[0073] In some embodiments, in step S1 and / or step S2, the coarse filtration is specifically carried out by filtering through a filter cloth with 200-400 meshes.

[0074] In some embodiments, in step S2, the stirring temperature is 25-35°C and the stirring time is 30-60 minutes.

[0075] In some embodiments, in step S2, the fine filtration is specifically carried out by filtering through a microporous membrane with a pore size of 0.2-0.6 μm.

[0076] The preparation method of the traditional Chinese medicine beverage for treating kidney-yang deficiency includes the following steps:

[0077] Step 1: Weigh the traditional Chinese medicine raw material powders of each component, mix them evenly to obtain traditional Chinese medicine powder;

[0078] Step 2: Place the traditional Chinese medicine powder in low-deuterium Taizisui water, stir evenly, let it stand to obtain swollen traditional Chinese medicine; put the swollen traditional Chinese medicine into a percolator, flatten it, and cover it with filter paper;

[0079] Step 3: Add low-deuterium Taizisui water to the percolator, open the outlet of the percolate, until the air bubbles in the cylinder are completely discharged, collect the flowing percolate and pour it back into the percolator, and add low-deuterium Taizisui water until the upper surface of the swollen traditional Chinese medicine is submerged by 6 - 10 cm, and soak for 20 - 30 h;

[0080] Step 4: Percolate at a flow rate of 1 - 3 ml / min, collect the percolate, disinfect and subpackage to obtain the traditional Chinese medicine beverage.

[0081] In some embodiments, in Step 1, the particle size of the traditional Chinese medicine powder is 75 - 250 μm; if the raw material crushing particles are too fine, they are prone to blockage and the leaching effect is poor; if they are too coarse, it is not easy to compact, and the contact area between the solvent and the medicinal materials is small.

[0082] In some embodiments, in Step 2, the mass of the low-deuterium Taizisui water used is 1 - 3 times the mass of the traditional Chinese medicine powder.

[0083] In some embodiments, in Step 4, the total addition amount of the swollen traditional Chinese medicine does not exceed 2 / 3 of the volume of the percolator.

[0084] Example

[0085] The following examples more specifically describe the content disclosed in the present invention. These examples are only for illustrative purposes, because various modifications and changes within the scope of the present invention are obvious to those skilled in the art. Unless otherwise stated, all parts, percentages, and ratios reported in the following examples are based on weight, and all reagents used in the examples are commercially available or can be synthesized according to conventional methods and can be used directly without further treatment, and the instruments used in the examples are all commercially available.

[0086] Example 1

[0087] A kind of low-deuterium Taizisui water, its preparation method is as follows:

[0088] S1: First, dissolve 1.4 parts of niobium acid and 0.7 parts of phosphotungstic acid in 400 parts of low-deuterium water, then add 0.3 parts of sodium pyrophosphate (a total of 2.4 parts of additives), after dissolution, soak 80 parts of Taizisui in it, place it in a dark environment, stir evenly, soak for 8 hours, and filter roughly through a double-layer 300-mesh filter cloth to obtain low-deuterium Taizisui crude liquid;

[0089] S2. Add 19 parts of activated carbon to the crude low-deuterium Ganoderma lucidum liquid, stir at 30 °C for 45 minutes, conduct rough filtration through a 200-mesh filter cloth, and then conduct fine filtration through a 0.45-μm microporous membrane to obtain low-deuterium Ganoderma lucidum water.

[0090] Calculated as follows:

[0091] In the low-deuterium Ganoderma lucidum water, the mass ratio of activated carbon to the total amount of niobic acid and phosphotungstic acid is 9.05:1;

[0092] The mass ratio of Ganoderma lucidum to low-deuterium water is 1:5;

[0093] The concentration of niobic acid is 0.35%;

[0094] The concentration of phosphotungstic acid is 0.18%;

[0095] The concentration of sodium pyrophosphate is 0.08%.

[0096] The dosage of the additive accounts for 0.60% of the total mass of the low-deuterium water.

[0097] Example 2

[0098] A kind of low-deuterium Ganoderma lucidum water, and its preparation method is as follows:

[0099] S1. First dissolve 0.42 parts of niobic acid and 0.13 parts of phosphotungstic acid in 150 parts of low-deuterium water, then add 0.13 parts of sodium pyrophosphate (a total of 0.68 parts of additives). After dissolution, immerse 50 parts of Ganoderma lucidum in it, place it in a dark environment, stir evenly, soak for 5 hours, and conduct rough filtration through a double-layer 300-mesh filter cloth to obtain a crude low-deuterium Ganoderma lucidum liquid;

[0100] S2. Add 4.8 parts of activated carbon to the crude low-deuterium Ganoderma lucidum liquid, stir at 25 °C for 60 minutes, conduct rough filtration through a 200-mesh filter cloth, and then conduct fine filtration through a 0.45-μm microporous membrane to obtain low-deuterium Ganoderma lucidum water.

[0101] Calculated as follows:

[0102] In the low-deuterium Ganoderma lucidum water, the mass ratio of activated carbon to the total amount of niobic acid and phosphotungstic acid is 8.73:1;

[0103] The mass ratio of Ganoderma lucidum to low-deuterium water is 1:3;

[0104] The concentration of niobic acid is 0.28%;

[0105] The concentration of phosphotungsten is 0.09%;

[0106] The concentration of sodium pyrophosphate is 0.09%;

[0107] The dosage of the additive accounts for 0.45% of the total mass of Ganoderma lucidum and low-deuterium water.

[0108] Example 3

[0109] A low-deuterium Taizui water, and its preparation method is as follows:

[0110] S1. First, dissolve 3.3 parts of niobic acid and 2.3 parts of phosphotungstic acid in 800 parts of low-deuterium water, then add 0.4 part of sodium pyrophosphate (a total of 6.0 parts of additives). After dissolution, immerse 100 parts of Taizui in it, place it in a dark environment, stir evenly, soak for 10 hours, and filter it roughly through a double-layer 300-mesh filter cloth to obtain a low-deuterium Taizui crude liquid;

[0111] S2. Add 52.5 parts of activated carbon to the low-deuterium Taizui crude liquid, stir for 30 minutes at 35°C, filter it roughly through a 200-mesh filter cloth, and then filter it precisely through a 0.45-μm microporous membrane to obtain low-deuterium Taizui water.

[0112] Calculated as follows:

[0113] In the low-deuterium Taizui water, the mass ratio of activated carbon to the total amount of niobic acid and phosphotungstic acid is 9.38:1;

[0114] The mass ratio of Taizui to low-deuterium water is 1:8;

[0115] The concentration of niobic acid is 0.41%;

[0116] The concentration of phosphotungstic acid is 0.29%;

[0117] The concentration of sodium pyrophosphate is 0.05%.

[0118] The dosage of the additives accounts for 0.75% of the total mass of Taizui and low-deuterium water.

[0119] Example 4

[0120] The preparation method of the Taizui low-deuterium water in Example 4 is basically the same as that in Example 1. The main difference is that the number of parts of niobic acid, phosphotungstic acid, and sodium pyrophosphate contained in the additives is different. Specifically, among the 2.4 parts of additives added, there are 0.8 part of niobic acid, 1.3 parts of phosphotungstic acid, and 0.3 part of sodium pyrophosphate.

[0121] Calculated as follows:

[0122] The concentration of niobic acid is 0.20%;

[0123] The concentration of phosphotungstic acid is 0.33%;

[0124] The concentration of sodium pyrophosphate is 0.08%.

[0125] Example 5

[0126] The preparation method of the Taizui low-deuterium water in Example 5 is basically the same as that in Example 1, and the main difference lies in the different proportions of niobic acid, phosphotungstic acid and sodium pyrophosphate contained in the additive. Specifically, among the 2.4 parts of the additive added, it includes 1.9 parts of niobic acid, 0.2 parts of phosphotungstic acid and 0.3 parts of sodium pyrophosphate.

[0127] After calculation:

[0128] The concentration of niobic acid is 0.48%;

[0129] The concentration of phosphotungstic acid is 0.05%;

[0130] The concentration of sodium pyrophosphate is 0.08%.

[0131] Comparative Example 1

[0132] The preparation method of Comparative Example 1 is basically the same as that of Example 1, and the main difference lies in the different proportions of niobic acid, phosphotungstic acid and sodium pyrophosphate contained in the additive. Specifically, among the 2.4 parts of the additive added, there is no niobic acid component, and it only includes 1.68 parts of phosphotungstic acid and 0.72 parts of sodium pyrophosphate.

[0133] Comparative Example 2

[0134] The preparation method of Comparative Example 2 is basically the same as that of Example 1, and the main difference lies in the different proportions of niobic acid, phosphotungstic acid and sodium pyrophosphate contained in the additive. Specifically, among the 2.4 parts of the additive added, there is no phosphotungstic acid component, and it only includes 1.98 parts of niobic acid and 0.42 parts of sodium pyrophosphate.

[0135] Comparative Example 3

[0136] The preparation method of Comparative Example 3 is basically the same as that of Example 1, and the main difference lies in the different proportions of niobic acid, phosphotungstic acid and sodium pyrophosphate contained in the additive. Specifically, among the 2.4 parts of the additive added, there is no sodium pyrophosphate component, and it only includes 1.6 parts of niobic acid and 0.8 parts of phosphotungstic acid.

[0137] Comparative Example 4

[0138] The preparation method of Comparative Example 4 is basically the same as that of Example 1, and the main difference lies in that in the additive, calcium niobate is used to replace niobic acid.

[0139] Comparative Example 5

[0140] The preparation method of Comparative Example 5 is basically the same as that of Example 1, and the main difference lies in that in the additive, citric acid is used to replace niobic acid.

[0141] Comparative Example 6

[0142] The preparation method of Comparative Example 6 is basically the same as that of Example 1, and the main difference lies in that in the additive, sodium hexametaphosphate is used to replace sodium pyrophosphate.

[0143] Test Example 1:

[0144] The active ingredients in the low-deuterium Taizui water obtained in Examples 1-5 and Comparative Examples 1-6 were detected. Among them, the detection of PQQ content: using the NBT-G1y method; the detection of fungal polysaccharide content: using the phenol-sulfuric acid method; the detection of nucleic acid content: using the phosphorus determination method; the detection of chitin content: referring to the method described in Chinese Patent 200910143787.6, and the detection data are listed in Table 1:

[0145] Table 1

[0146] PQQ content Fungal polysaccharide content Nucleic acid content Chitin content Example 1 108 3063 439 271 Example 2 103 3005 414 253 Example 3 110 3116 450 280 Example 4 82 2795 373 250 Example 5 87 2890 390 263 Comparative example 1 56 2314 301 197 Comparative example 2 65 2525 332 218 Comparative example 3 69 2593 348 233 Comparative example 4 34 1998 264 176 Comparative example 5 80 2770 373 244 Comparative example 6 73 2657 366 235

[0147] It can be seen from the data in Table 1 that in Examples 1-3, the PQQ content in the low-deuterium Taizui water is higher than 103 μg / mL, the fungal polysaccharide content is higher than 3005 μg / mL, the nucleic acid content is higher than 414 μg / mL, and the chitin content is higher than 253 μg / mL. It can be seen that the leaching rate of the active ingredients in the low-deuterium Taizui water is relatively excellent. The combined use of niobic acid, phosphotungstic acid and sodium pyrophosphate can promote the leaching of the active ingredients in Taizui.

[0148] Compared with Example 1, the content of the active ingredients in the low-deuterium Taizui water in Example 4 decreased. The reason may be that: the concentration of niobic acid decreased, and its softening effect on the Taizui cell wall became weaker, which affected the cell wall breaking behavior of phosphotungstic acid and sodium pyrophosphate to a certain extent. Therefore, the leaching rate of the active ingredients in the Taizui water decreased.

[0149] Compared with Example 1, the content of the active ingredients in the low-deuterium Taizui water in Example 5 decreased. The reason may be that: the concentration of niobic acid was relatively high. Although it could improve the softening degree of the cell wall, the decrease in the concentration of phosphotungstic acid led to the weakening of its oxidative chain-breaking effect on the area after the action of niobic acid, which further affected the leaching of the active substances in Taizui. Therefore, the content of the active ingredients in the low-deuterium Taizui water decreased.

[0150] Compared with Example 1, the content of the active ingredients in the low-deuterium Taizui water in Comparative Example 1 decreased significantly. The reason may be that: the lack of niobic acid significantly reduced the softening effect on the Taizui cell wall, reduced the cell wall breaking efficiency of phosphotungstic acid and sodium pyrophosphate, and led to the decrease in the dissolution efficiency of the active ingredients in the low-deuterium Taizui. Therefore, the content of the active ingredients in the low-deuterium Taizui water decreased.

[0151] Compared with Example 1, the content of the active ingredients in the low-deuterium Taizui water in Comparative Example 2 decreased significantly. The reason may be that: the lack of phosphotungstic acid weakened the attacking effect on the cell wall softened by niobic acid, resulting in a significant decrease in the dissolution efficiency of the active ingredients in Taizui. Therefore, the leaching rate of the active ingredients in the low-deuterium Taizui water decreased.

[0152] Compared with Example 1, the content of active ingredients in the low-deuterium Taizui water in Comparative Example 3 decreased significantly. The reason may be that: lacking sodium pyrophosphate, it cannot enhance the weakening effect on the mechanical strength of the Taizui cell wall, and the synergistic effect with niobic acid and phosphotungstic acid becomes poor, which is not conducive to the dissolution of active substances in Taizui. Therefore, the content of active ingredients in the low-deuterium Taizui water decreased significantly.

[0153] Compared with Example 1, the content of active ingredients in the low-deuterium Taizui water in Comparative Example 4 decreased significantly. The reason may be that: in the additive, niobic acid was replaced with calcium niobate, which does not have acidity, cannot soften the Taizui cell wall, and cannot provide support for the precise oxidative chain-breaking effect of phosphotungstic acid, which is not conducive to the leaching of active substances in Taizui. Therefore, the leaching rate of active ingredients in the Taizui water decreased significantly.

[0154] From the test results of Comparative Example 5, although niobic acid in the additive was replaced with citric acid, which is theoretically more conducive to the leaching of active ingredients in Taizui, the content of active ingredients in the obtained low-deuterium Taizui water is not high. This may be because citric acid cannot cooperate with phosphotungstic acid and sodium pyrophosphate to promote the leaching of active substances; similarly, in Comparative Example 6, sodium pyrophosphate in the additive was replaced with sodium hexametaphosphate, and the test results of active ingredients were not optimistic; it can be seen that niobic acid, phosphotungstic acid, and sodium pyrophosphate in the additive of the present application have good synergistic effects and can better promote the leaching of active substances in Taizui.

[0155] Application Example 1

[0156] A traditional Chinese medicine beverage for treating kidney-yang deficiency, and its preparation method includes the following steps:

[0157] Step 1: Weigh 1.4 parts of dendrobium powder, 4.2 parts of wolfberry powder, 1.4 parts of ginseng powder, 4 parts of astragalus powder, 2.8 parts of cornel powder, 5.2 parts of roasted licorice powder, 4.4 parts of polygonatum powder, and 2.8 parts of cistanche powder, mix them evenly to obtain traditional Chinese medicine powder, and the particle size of the traditional Chinese medicine powder is 150 μm.

[0158] Step 2: Place the traditional Chinese medicine powder in the low-deuterium Taizui water prepared in Example 1, stir evenly, and let it stand for 8 h to obtain swollen traditional Chinese medicine; put the swollen traditional Chinese medicine into a percolator, flatten it, and cover it with filter paper. Among them, the mass of the low-deuterium Taizui water is 1.5 times the mass of the traditional Chinese medicine powder.

[0159] Step 3: Add the low-deuterium Taizui water prepared in Example 1 to the percolator, open the outlet of the percolate, until the bubbles in the cylinder are completely removed, collect the flowing percolate and pour it back into the percolator, and add low-deuterium Taizui water until the upper surface of the swollen traditional Chinese medicine is immersed by 8 cm, and soak for 25 h.

[0160] Step 4: Percolate at a flow rate of 2 ml / min, collect the percolate, disinfect and subpackage it to obtain the traditional Chinese medicine beverage.

[0161] Application Example 2

[0162] A Chinese medicinal beverage for treating kidney-yang deficiency, the preparation method of which comprises the following steps:

[0163] Step 1, weigh 1 part of dendrobium powder, 3 parts of wolfberry powder, 1 part of ginseng powder, 2.8 parts of astragalus powder, 2 parts of cornus powder, 3.7 parts of licorice powder, 3.1 parts of polygonatum powder, 2 parts of cistanche powder, mix well to obtain Chinese medicine powder, the particle size of the Chinese medicine powder is 75μm.

[0164] Step 2: Place the Chinese medicine powder in the low-deuterium water prepared in Example 1, stir evenly, and let it stand for 5 hours to obtain expanded Chinese medicine; put the expanded Chinese medicine into a percolation cylinder, flatten it, and cover it with filter paper. The mass of the low-deuterium water is 1.5 times the mass of the Chinese medicine powder.

[0165] Step 3: Add the deuterium-depleted water prepared in Example 1 to the percolation cylinder, open the percolation liquid outlet until the bubbles in the cylinder are completely eliminated, collect the outflowing percolation liquid and pour it into the percolation cylinder, and add deuterium-depleted water until the upper surface of the swollen Chinese medicine is immersed by 6 cm, and soak for 20 hours.

[0166] Step 4: percolating at a flow rate of 1 ml / min, collecting the percolating liquid, sterilizing and packaging, and thus preparing the Chinese medicine beverage.

[0167] Application Example 3

[0168] A Chinese medicinal beverage for treating kidney-yang deficiency, the preparation method of which comprises the following steps:

[0169] Step 1, weigh 2 parts of dendrobium powder, 6 parts of wolfberry powder, 2 parts of ginseng powder, 5.6 parts of astragalus powder, 4 parts of cornus powder, 7.4 parts of licorice powder, 6.2 parts of polygonatum powder, 4 parts of cistanche powder, mix well to obtain Chinese medicine powder, the particle size of the Chinese medicine powder is 250μm.

[0170] Step 2: Place the Chinese medicine powder in the low-deuterium water prepared in Example 1, stir evenly, and let it stand for 10 hours to obtain expanded Chinese medicine; put the expanded Chinese medicine into a percolation cylinder, flatten it, and cover it with filter paper. The mass of the low-deuterium water is 1.5 times the mass of the Chinese medicine powder.

[0171] Step 3: Add deuterium-depleted water to the percolation cylinder, open the percolation liquid outlet until the bubbles in the cylinder are completely eliminated, collect the outflowing percolation liquid and pour it into the percolation cylinder, and add deuterium-depleted water until the upper surface of the swollen Chinese medicine is immersed by 10 cm, and soak for 30 hours.

[0172] Step 4: percolating at a flow rate of 3 ml / min, collecting the percolating liquid, sterilizing and packaging, and thus obtaining the Chinese medicine beverage.

[0173] Test Example 2

[0174] Test the therapeutic effects of the traditional Chinese medicine beverage prepared in Application Example 1 and Jinkui Shenqi Pills.

[0175] Test subjects: Select 70 patients with yang deficiency and decline who meet the standards. All the selected cases are from the outpatients and inpatients in the Nephrology Department of Shijiazhuang Hospital of Traditional Chinese Medicine from April 2018 to March 2019, who meet the symptoms of soreness and weakness in the waist, emptiness in the waist, coldness in the waist, cold hands and feet, coldness in the lower abdomen, frequent nocturia, hair loss, and white hair due to yang deficiency and decline.

[0176] Screening conditions for test subjects: Aged 18 - 60 years old; without acute and critical illnesses; voluntarily sign the informed consent form. Exclude those who are participating in other clinical studies, patients with severe complications in hematopoiesis, liver, nervous system, heart, etc., those with a history of allergy or allergy to the drugs involved in this application, and those who have been treated with other similar drugs.

[0177] Experiment termination criteria: Terminate the experiment for those who change drugs on their own during the experiment, use drugs prohibited during the experiment, those who are unwilling to continue to cooperate with clinical treatment during the experiment, those who cannot take medicine on time according to the course of treatment, have poor compliance and drop out or are lost to follow - up, and those who have severe adverse reactions.

[0178] Randomly divide the test patients into a traditional Chinese medicine beverage adjuvant treatment group and a Jinkui Shenqi Pills control group. Among them, there are 35 patients in the traditional Chinese medicine beverage adjuvant treatment group, including 20 male patients and 15 female patients, with an average age of 56 years. While receiving the basic treatment of Jinkui Shenqi Pills, give the traditional Chinese medicine beverage prepared in Application Example 1, 150 ml each time, three times a day; there are 35 patients in the Jinkui Shenqi Pills control group, including 18 male patients and 17 female patients, with an average age of 54 years, and only give the basic treatment of Jinkui Shenqi Pills. The treatment cycle for both groups is 30 days, and observe the evaluation of the curative effect of traditional Chinese medicine syndromes before and after treatment in both groups.

[0179] The evaluation criteria for the curative effect of traditional Chinese medicine syndromes refer to the syndrome curative effect evaluation criteria in the "Guiding Principles for Clinical Research of New Traditional Chinese Medicines", and score the traditional Chinese medicine syndromes before and after treatment. Each symptom is divided into 4 levels: none, mild, moderate, and severe. Main symptoms: No symptom is recorded as 0 points; mild symptom is recorded as 2 points; moderate symptom is recorded as 4 points; severe symptom is recorded as 6 points. Sub - symptoms: No symptom is recorded as 0 points; mild symptom is recorded as 1 point; moderate symptom is recorded as 2 points; severe symptom is recorded as 3 points. The cumulative sum of each score is recorded as the total score of the traditional Chinese medicine syndromes of the patient. Note: The calculation formula (Nimodipine method) is: [(score before treatment - score after treatment) / score before treatment]×100%. The treatment effect is shown in Table 2:

[0180] Table 2

[0181]

[0182] From the treatment data in Table 2, it can be seen that low-deuterium Tai Sui water synergistically with the traditional Chinese medicine formula significantly reduced the symptom score (the average improvement rate was about 38.6%), especially for "empty waist" and "white tongue coating, deep and weak pulse". The improvement rate of traditional Jin Kui Shen Qi Pills was about 28.1%, and the effect was relatively mild.

[0183] Therefore, the Chinese medicinal beverage of the present invention can effectively treat the symptoms such as soreness of waist and knees, hair loss, cold hands and feet caused by spleen and kidney yang deficiency, and has no side effects on the human body, so patients are willing to accept the treatment.

[0184] The above implementation modes are only for illustrating the technical concept and features of the present invention, and their purpose is to enable people familiar with this technology to understand the content of the present invention and implement it, and they cannot be used to limit the protection scope of the present invention. Any equivalent changes or modifications made according to the spirit of the present invention should be included in the protection scope of the present invention.

Claims

1. A low-deuterium water, characterized in that: By weight, the raw materials for preparing the low-deuterium Taizui water include 150 - 800 parts of low-deuterium water, 50 - 100 parts of Taizui, and 0.5 - 10 parts of additive; The additive includes niobic acid, phosphotungstic acid, and sodium pyrophosphate.

2. The low-deuterium Ganoderma water according to claim 1, characterized in that, By weight, the additive includes 0.4 - 3.5 parts of niobic acid, 0.1 - 2.5 parts of phosphotungstic acid, and 0.1 - 0.5 parts of sodium pyrophosphate; The concentration of the niobic acid is 0.1 - 0.6%; The concentration of the phosphotungstic acid is 0.01 - 0.4%; The concentration of the sodium pyrophosphate is 0.01 - 0.12%.

3. The low-deuterium Taizui water according to claim 1, characterized in that The concentration of the niobic acid is 0.25 - 0.45%; The concentration of the phosphotungstic acid is 0.08 - 0.3%; The concentration of the sodium pyrophosphate is 0.05 - 0.1%.

4. The low-deuterium Ganoderma lucidum water according to claim 1, characterized in that, By weight, the raw materials for preparing the low-deuterium Taizui water further include 4 - 53 parts of activated carbon.

5. The low-deuterium Taizui water according to claim 1, characterized in that The mass ratio of the Taizui to the low-deuterium water is 1:(3 - 8); The dosage of the additive does not exceed 1% of the total mass of the Taizui and the low-deuterium water; The mass ratio of the activated carbon to the total amount of the niobic acid and the phosphotungstic acid is (8 - 10):

1.

6. The low-deuterium water according to any one of claims 1 to 5, characterized in that: The preparation method includes the following steps: S1. Dissolve the additive in the low-deuterium water, then soak the Taizui in the low-deuterium water, place it in a dark environment, stir evenly, soak, and obtain the low-deuterium Taizui crude liquid through rough filtration; S2. Add activated carbon to the low-deuterium Taizui crude liquid, stir evenly, and obtain the low-deuterium Taizui water through rough filtration and fine filtration.

7. A traditional Chinese medicine beverage for treating kidney-yang deficiency, characterized in that, The raw materials for preparing the traditional Chinese medicine beverage include the low-deuterium Taizui water according to any one of claims 1 - 5 and traditional Chinese medicine powder. The traditional Chinese medicine powder includes 0.5 - 3 parts of dendrobium powder, 2 - 8 parts of wolfberry powder, 0.5 - 3 parts of ginseng powder, 1 - 9 parts of astragalus powder, 1 - 7 parts of cornel powder, 1 - 10 parts of roasted licorice powder, 1 - 9 parts of polygonatum powder, and 1 - 6 parts of cistanche powder.

8. The traditional Chinese medicine beverage for treating kidney-yang deficiency according to claim 7, wherein, The traditional Chinese medicine powder includes 1 - 2 parts of dendrobium powder, 3 - 6 parts of wolfberry powder, 1 - 2 parts of ginseng powder, 2 - 6 parts of astragalus powder, 2 - 4 parts of cornel powder, 3 - 8 parts of roasted licorice powder, 3 - 7 parts of polygonatum powder, and 2 - 4 parts of cistanche powder.

9. A method for preparing a traditional Chinese medicine beverage for treating kidney-yang deficiency according to any one of claims 7 or 8, characterized in that, It includes the following steps: Step 1. Weigh the traditional Chinese medicine raw material powders of each component, mix them evenly to obtain the traditional Chinese medicine powder; Step 2. Place the traditional Chinese medicine powder in the low-deuterium Taizui water, stir evenly, let it stand to obtain the swollen traditional Chinese medicine; put the swollen traditional Chinese medicine into a percolator, flatten it, and cover it with filter paper; Step 3. Add low-deuterium Taizui water to the percolator, open the outlet of the percolate, collect the flowing percolate and pour it back into the percolator, and add low-deuterium Taizui water until the low-deuterium Taizui water submerges the swollen traditional Chinese medicine and fully impregnates it; Step 4. Percolate, collect the percolate, and prepare the traditional Chinese medicine beverage.

10. The preparation method of the traditional Chinese medicine beverage for treating kidney-yang deficiency according to claim 9, characterized in that, In step 1, the particle size of the traditional Chinese medicine powder is 75 - 250 μm; In step 2, the mass of the low-deuterium Taizui water is 1 - 3 times the mass of the traditional Chinese medicine powder; In step 3, the height of the low-deuterium Taizui water submerging the upper surface of the swollen traditional Chinese medicine is 6 - 10 cm.

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