Device for collecting protein secreted by stem cell differentiation based on precipitation method

Abstract

The invention relates to the technical field of protein collecting devices, and discloses a protein collecting device secreted by stem cell differentiation based on a precipitation method, the protein collecting device comprises a scraping plate, a guide groove is formed in the surface of the scraping plate, and the bottom of the scraping plate is fixedly connected with a flow guide groove plate; by arranging the scraping mechanism, when a scraping plate rotates, the inner wall of the collecting barrel is scraped, when the scraping plate rotates, residual protein on the inner wall of the collecting barrel is scraped to the inner wall of a guide groove through rotation inertia, and when the protein flows to the guide groove, the protein flows downwards along the guide groove, so that the protein is separated from the collecting barrel. The protein on the inner wall of the guide groove quickly flows downwards in cooperation with vibration sense generated by impact of the contact telescopic rod on the surface of the collecting barrel, so that the protein on the inner wall of the collecting barrel is quickly collected, the residual protein is effectively collected, damage is reduced, the inner wall of the collecting barrel is cleaned through a cleaning brush, and the working efficiency is improved. The cleaning time is saved, and the efficiency is improved for the next use.

Description

Technical Field

[0001] The present invention relates to the technical field of protein collection equipment, and particularly to a protein collection device for collecting secreted proteins generated by stem cell differentiation based on the precipitation method. Background Technique

[0002] Proteins are one of the most basic molecular components in organisms and play an important role in regulating cell functions and signal transduction. Proteomics plays an important role in biopharmaceutical research and development and can be used to identify and quantify drug candidates, monitor protein expression and quality consistency, etc. The application of proteomics can improve the efficiency and success rate of drug research and development.

[0003] In the prior art, after the protein is precipitated, it needs to be collected. However, after the protein is taken away from the collection device, some protein residues will still remain inside the collection device and adhere to the inner wall of the collection device. Over time, these residual proteins will be damaged, and at this time, the residual proteins need to be collected, and manual collection is likely to damage the proteins. Summary of the Invention

[0004] The purpose of the present invention is to provide a protein collection device for collecting secreted proteins generated by stem cell differentiation based on the precipitation method to solve the problems raised in the above background technique.

[0005] To solve the above technical problems, the present invention is realized through the following technical solutions: The present invention is a protein collection device for collecting secreted proteins generated by stem cell differentiation based on the precipitation method, including a collection cylinder. A support foot is fixedly connected to the surface of the collection cylinder, a bottom plate is fixedly connected to the bottom of the support foot, a support frame is fixedly connected to the surface of the bottom plate, and a motor is fixedly connected to the top of the support frame. It further includes; A vibration mechanism, which includes a moving plate. A driven push rod is rotatably connected to the surface of the moving plate, and a contact telescopic rod is rotatably connected to the end of the driven push rod away from the moving plate. A scraping mechanism, which includes a scraper. A guiding groove is provided on the surface of the scraper, and a diversion groove plate is fixedly connected to the bottom of the scraper. A collection mechanism, which includes a threaded plate. Scraping rings are fixedly connected to both ends of the threaded plate, and a push plate is rotatably connected to the bottom of the threaded plate.

[0006] Furthermore, the number of support frames is set to four, and the four support frames are symmetrically arranged with the bottom plate as the center. A storage hole is provided at the bottom of the inner wall of the collection cylinder, and the number of support feet is set to three.

[0007] Furthermore, the vibration mechanism includes a transmission shaft. A threaded ring is fixedly connected to the surface of the transmission shaft. A rotating ring is fixedly connected to the surface of the threaded ring. An extrusion bent rod is fixedly connected to the bottom of the rotating ring. A chute plate is fixedly connected to the inner wall of the support frame. A force-receiving inclined plate is fixedly connected to the top of the moving plate. A return spring is fixedly connected to the bottom of the moving plate.

[0008] Furthermore, the end of the transmission shaft is fixedly connected to the output end of the motor. Both ends of the moving plate are slidably connected to the inner wall of the chute plate. The end of the extrusion bent rod away from the rotating ring contacts the surface of the force-receiving inclined plate. The end of the contact telescopic rod away from the driven push rod is fixedly connected to the inner wall of the support frame.

[0009] Furthermore, the scraping mechanism includes a rotating shaft. A driven ring is fixedly connected to the surface of the rotating shaft. A rotating rod is fixedly connected to the surface of the driven ring. A cleaning plate is fixedly connected to the end of the rotating rod away from the driven ring. A cleaning brush is fixedly connected to the surface of the cleaning plate.

[0010] Furthermore, the end of the rotating shaft is fixedly connected to the end of the transmission shaft. A scraper is fixedly connected to the surface of the rotating rod. One side of the scraper away from the rotating rod contacts the inner wall of the collection cylinder. The end of the cleaning brush away from the cleaning plate contacts the inner wall of the collection cylinder.

[0011] Furthermore, the collection mechanism includes a threaded rod. A collection shrinkage ring is rotatably connected to the end of the push plate away from the threaded plate. A limit spring is fixedly connected to the bottom of the threaded plate. A storage box is arranged at the bottom of the collection cylinder. A drainage inclined plate is fixedly connected to the inner wall of the collection cylinder.

[0012] Furthermore, the end of the rotating shaft away from the transmission shaft is fixedly connected to the end of the threaded rod. The bottom of the collection shrinkage ring contacts the bottom of the inner wall of the collection cylinder. The outer wall of the scraping ring contacts the inner wall of the collection cylinder. The end of the threaded rod away from the rotating shaft is rotatably connected to the inner wall of the collection cylinder. The top of the storage box contacts the bottom of the collection cylinder. The bottom of the storage box contacts the surface of the bottom plate.

[0013] The present invention has the following beneficial effects: In the present invention, by providing a vibration mechanism, the motor is first started to drive the transmission shaft to rotate. When the transmission shaft rotates, it drives the threaded ring to rotate. When the threaded ring rotates, it drives the rotating ring to rotate. When the rotating ring rotates, it squeezes the force-bearing inclined plate. When the force-bearing inclined plate is squeezed, it pushes the moving plate to slide downward along the inner wall of the chute plate. When the moving plate slides, it pushes the driven push rod to move away from each other. When the driven push rod moves, it pushes the contact telescopic rod to extend towards each other and contact the surface of the collection cylinder. When the moving plate slides, it squeezes the return spring. When the return spring is squeezed, it contracts downward. When the squeezing of the force-bearing inclined plate by the squeezing bent rod ends, at this time, the moving plate has no squeezing force and will return to its original position through the elastic force of the return spring. At the same time, the driven push rod will pull the contact telescopic rod back to its original position. Since the transmission shaft drives the threaded ring to keep rotating, and at the same time the rotating ring drives the squeezing bent rod to keep rotating and squeezes the force-bearing inclined plate. Since a plurality of squeezing bent rods are provided and there is a certain distance between each squeezing bent rod, at this time, the force-bearing inclined plate will be squeezed, so that the force-bearing inclined plate pushes the moving plate to slide reciprocally. When the moving plate slides reciprocally, it pushes the driven push rod to move reciprocally. At the same time, the driven push rod pushes the contact telescopic rod to extend reciprocally and slightly impacts the surface of the collection cylinder, so that the inner wall of the collection cylinder generates a vibration feeling, and the protein adhering to the inner wall of the collection cylinder flows downward when it feels the vibration, thereby improving the efficiency of collecting the protein remaining on the inner wall of the collection cylinder.

[0014] In the present invention, by providing a scraping mechanism, when the transmission shaft rotates, it drives the rotating shaft to rotate. When the rotating shaft rotates, it drives the driven ring to rotate. When the driven ring rotates, it drives the rotating rod to rotate. When the rotating rod rotates, it drives the cleaning plate to rotate. When the cleaning plate rotates, it drives the cleaning brush to rotate, thereby cleaning the inner wall of the collection cylinder and saving labor and improving the cleaning efficiency. When the bottom plate rotates, it drives the scraping plate to rotate. When the scraping plate rotates, it scrapes the inner wall of the collection cylinder. When the scraping plate rotates, due to the inertia of rotation, the protein remaining on the inner wall of the collection cylinder is scraped onto the inner wall of the guide groove. When the protein flows into the guide groove, it flows downward along the guide groove, and cooperates with the vibration feeling generated by the impact of the contact telescopic rod on the surface of the collection cylinder, so that the protein on the inner wall of the guide groove flows downward quickly, thereby quickly collecting the protein on the inner wall of the collection cylinder. It not only effectively collects the remaining protein and reduces damage, but also cleans the inner wall of the collection cylinder with the cleaning brush, saving the cleaning time and improving the efficiency for the next use.

[0015] In the present invention, by providing a collection mechanism, when the rotating shaft rotates, it drives the threaded rod to rotate. When the threaded rod rotates, it causes the threaded plate to move downward. When the threaded plate moves, it pulls the scraping ring downward. When the scraping ring moves, it scrapes the lower surface of the inner wall of the collection cylinder downward, scraping the protein on the inner wall downward, and guiding the protein to flow downward through the diversion inclined plate. At this time, the threaded plate moves to a position where there is no thread on the surface of the threaded rod, and the threaded plate is limited by the limiting spring to keep stationary. At this time, the threaded rod continues to rotate, and at the same time, the scraping ring moves to above the diversion inclined plate and remains stationary, not contacting the diversion inclined plate. When the scraping ring moves downward, it pushes the push plate downward. When the push plate moves, it pushes the collection shrinkage ring to shrink in the direction of approaching each other, thereby concentrating the flowing protein and pushing it to the inner wall of the storage box, effectively achieving rapid collection of the residual protein and reducing protein damage.

[0016] Of course, it is not necessary for any product implementing the present invention to achieve all the above advantages simultaneously. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following will briefly introduce the drawings required for the description of the embodiments. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained based on these drawings.

[0018] Figure 1 Schematic diagram of the overall structure of the present invention; Figure 2 Schematic cross-sectional view of the overall structure of the present invention; Figure 3 Schematic diagram of the overall structure of the vibration mechanism of the present invention; Figure 4 Schematic diagram of the structure of the driven push rod of the present invention; Figure 5 Schematic diagram of the overall structure of the scraping mechanism of the present invention; Figure 6 Schematic diagram of the structure of the diversion groove plate of the present invention; Figure 7 Schematic diagram of the overall structure of the collection mechanism of the present invention; Figure 8 Schematic diagram of the structure of the scraping ring of the present invention; Figure 9 Schematic diagram of the structure of the storage box of the present invention.

[0019] In the drawings, the list of components represented by each reference numeral is as follows: In the figure: 1. Collection cylinder; 2. Support feet; 3. Bottom plate; 4. Support frame; 5. Motor; 10. Vibration mechanism; 11. Transmission shaft; 12. Threaded ring; 13. Rotating ring; 14. Extrusion bent rod; 15. Chute plate; 16. Moving plate; 17. Force-receiving inclined plate; 18. Driven push rod; 19. Contact telescopic rod; 20. Return spring; 30. Scraping mechanism; 31. Rotating shaft; 32. Driven ring; 33. Rotating rod; 34. Cleaning plate; 35. Cleaning brush; 36. Scraper; 37. Flow guide groove plate; 50. Collection mechanism; 51. Threaded rod; 52. Threaded plate; 53. Scraping ring; 54. Pushing plate; 55. Collection shrinkage ring; 56. Limit spring; 57. Storage box; 58. Drainage inclined plate. Detailed implementation manners

[0020] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0021] Please refer to Figure 1 - Figure 9 As shown in the figure, the present invention is a protein collection device for the secretion generated by the differentiation of stem cells based on the precipitation method, including a collection cylinder 1. Support feet 2 are fixedly connected to the surface of the collection cylinder 1. Bottom plates 3 are fixedly connected to the bottoms of the support feet 2. Support frames 4 are fixedly connected to the surfaces of the bottom plates 3. Motors 5 are fixedly connected to the tops of the support frames 4. It also includes; A vibration mechanism 10. The vibration mechanism 10 includes a moving plate 16. When the force-receiving inclined plate 17 is squeezed, it will push the moving plate 16 to slide downward on the inner wall of the chute plate 15. A driven push rod 18 is rotatably connected to the surface of the moving plate 16. When the moving plate 16 slides, it will push the driven push rod 18 to move away from each other. The end of the driven push rod 18 away from the moving plate 16 is rotatably connected to a contact telescopic rod 19. When the driven push rod 18 moves, it will push the contact telescopic rod 19 to extend towards each other and contact the surface of the collection cylinder 1; A scraping mechanism 30. The scraping mechanism 30 includes a scraper 36. When the bottom plate 3 rotates, it will drive the scraper 36 to rotate. A guiding groove is formed on the surface of the scraper 36. When the scraper 36 rotates, it will scrape the inner wall of the collection cylinder 1. When the scraper 36 rotates, due to the inertia of rotation, the residual protein on the inner wall of the collection cylinder 1 will be scraped onto the inner wall of the guiding groove. When the protein flows into the guiding groove, it will flow downward along the guiding groove and cooperate with the vibration generated by the impact of the contact telescopic rod 19 on the surface of the collection cylinder 1, so that the protein on the inner wall of the guiding groove flows downward quickly. A flow guide groove plate 37 is fixedly connected to the bottom of the scraper 36; The collecting mechanism 50 includes a threaded plate 52. When the threaded rod 51 rotates, it will cause the threaded plate 52 to move downward. Both ends of the threaded plate 52 are fixedly connected with scraping rings 53. When the threaded plate 52 moves, it will pull the scraping rings 53 to move downward. When the scraping rings 53 move, they will scrape the lower surface of the inner wall of the collecting cylinder 1 downward, scraping the protein on the inner wall downward. The bottom of the threaded plate 52 is rotatably connected with a pushing plate 54. When the scraping rings 53 move downward, they will push the pushing plate 54 to move downward at the same time.

[0022] The number of the support frames 4 is set to four, and the four support frames 4 are symmetrically arranged with the bottom plate 3 as the center. A storage hole is provided at the bottom of the inner wall of the collecting cylinder 1, and the number of the support feet 2 is set to three.

[0023] The vibration mechanism 10 includes a transmission shaft 11. First, start the motor 5 to drive the transmission shaft 11 to rotate. A threaded ring 12 is fixedly connected to the surface of the transmission shaft 11. When the transmission shaft 11 rotates, it will drive the threaded ring 12 to rotate. A rotating ring 13 is fixedly connected to the surface of the threaded ring 12. When the threaded ring 12 rotates, it will drive the rotating ring 13 to rotate. An extrusion bent rod 14 is fixedly connected to the bottom of the rotating ring 13. At the same time, the rotating ring 13 will drive the extrusion bent rod 14 to keep rotating and extrude the force-receiving inclined plate 17. A chute plate 15 is fixedly connected to the inner wall of the support frame 4. A force-receiving inclined plate 17 is fixedly connected to the top of the moving plate 16. When the rotating ring 13 rotates, it will extrude the force-receiving inclined plate 17. A return spring 20 is fixedly connected to the bottom of the moving plate 16. When the moving plate 16 slides, it will squeeze the return spring 20. When the return spring 20 is squeezed, it will contract downward. When the moving plate 16 slides back and forth, it will push the driven push rod 18 to move back and forth. At the same time, the driven push rod 18 will push the contact telescopic rod 19 to extend and retract back and forth, and slightly impact the surface of the collecting cylinder 1, so as to generate a vibration feeling on the inner wall of the collecting cylinder 1, making the protein adhering to the inner wall of the collecting cylinder 1 flow downward when feeling the vibration, thereby improving the efficiency of collecting the protein remaining on the inner wall of the collecting cylinder 1.

[0024] The end of the transmission shaft 11 is fixedly connected to the output end of the motor 5. Since the transmission shaft 11 will drive the threaded ring 12 to keep rotating, both ends of the moving plate 16 slide with the inner wall of the chute plate 15. At this time, the moving plate 16 has no extrusion force and will be reset to its original position by the elastic force of the return spring 20. The end of the extrusion bent rod 14 away from the rotating ring 13 contacts the surface of the force-receiving inclined plate 17. Since a number of extrusion bent rods 14 are provided and there is a certain distance between each extrusion bent rod 14, at this time, the force-receiving inclined plate 17 will be extruded, so that the force-receiving inclined plate 17 pushes the moving plate 16 to slide back and forth. When the extrusion of the force-receiving inclined plate 17 by the extrusion bent rod 14 ends, the end of the contact telescopic rod 19 away from the driven push rod 18 is fixedly connected to the inner wall of the support frame 4. At the same time, the driven push rod 18 will pull the contact telescopic rod 19 back to its original position.

[0025] The scraping mechanism 30 includes a rotating shaft 31. When the transmission shaft 11 rotates, it drives the rotating shaft 31 to rotate. A driven ring 32 is fixedly connected to the surface of the rotating shaft 31. When the rotating shaft 31 rotates, it drives the driven ring 32 to rotate. A rotating rod 33 is fixedly connected to the surface of the driven ring 32. One end of the rotating rod 33 away from the driven ring 32 is fixedly connected to a cleaning plate 34. A cleaning brush 35 is fixedly connected to the surface of the cleaning plate 34. When the cleaning plate 34 rotates, it drives the cleaning brush 35 to rotate, thereby cleaning the inner wall of the collection cylinder 1, saving labor and improving the cleaning efficiency.

[0026] The end of the rotating shaft 31 is fixedly connected to the end of the transmission shaft 11. The surface of the scraping plate 36 is fixedly connected to the surface of the rotating rod 33. When the driven ring 32 rotates, it drives the rotating rod 33 to rotate. One side of the scraping plate 36 away from the rotating rod 33 contacts the inner wall of the collection cylinder 1. One end of the cleaning brush 35 away from the cleaning plate 34 contacts the inner wall of the collection cylinder 1. When the rotating rod 33 rotates, it drives the cleaning plate 34 to rotate, thereby quickly collecting the protein on the inner wall of the collection cylinder 1. It not only effectively collects the residual protein and reduces damage, but also cleans the inner wall of the collection cylinder 1 through the cleaning brush 35, saving the cleaning time and improving the efficiency for the next use.

[0027] The collection mechanism 50 includes a threaded rod 51. At this time, the threaded plate 52 moves to the position on the surface of the threaded rod 51 where there is no thread. When the rotating shaft 31 rotates, it drives the threaded rod 51 to rotate. One end of the pushing plate 54 away from the threaded plate 52 is rotatably connected to a collection shrinkage ring 55. A limiting spring 56 is fixedly connected to the bottom of the threaded plate 52. A storage box 57 is provided at the bottom of the collection cylinder 1. A drainage inclined plate 58 is fixedly connected to the inner wall of the collection cylinder 1, and the protein is guided to flow downward through the drainage inclined plate 58.

[0028] One end of the rotating shaft 31 away from the transmission shaft 11 is fixedly connected to the end of the threaded rod 51. The threaded plate 52 is kept stationary by being limited by the limiting spring 56. At this time, the threaded rod 51 keeps rotating. The bottom of the collection shrinkage ring 55 contacts the bottom of the inner wall of the collection cylinder 1. The outer wall of the scraping ring 53 contacts the inner wall of the collection cylinder 1. At the same time, the scraping ring 53 moves above the drainage inclined plate 58 and remains stationary without contacting the drainage inclined plate 58. One end of the threaded rod 51 away from the rotating shaft 31 is rotatably connected to the inner wall of the collection cylinder 1. The top of the storage box 57 contacts the bottom of the collection cylinder 1. The bottom of the storage box 57 contacts the surface of the bottom plate 3. When the pushing plate 54 moves, it pushes the collection shrinkage ring 55 to contract in the direction of approaching each other, thereby concentrating the flowing-down protein and pushing it into the inner wall of the storage box 57, effectively achieving the rapid collection of the residual protein and reducing the damage of the protein.

[0029] During use, first start the motor 5 to drive the transmission shaft 11 to rotate. When the transmission shaft 11 rotates, it will drive the threaded ring 12 to rotate. When the threaded ring 12 rotates, it will drive the rotating ring 13 to rotate. When the rotating ring 13 rotates, it will squeeze the force-bearing inclined plate 17. When the force-bearing inclined plate 17 is squeezed, it will push the moving plate 16 to slide downward along the inner wall of the chute plate 15. When the moving plate 16 slides, it will push the driven push rod 18 to move in a mutually separated direction. When the driven push rod 18 moves, it will push the contact telescopic rod 19 to extend in a mutually approaching direction and contact the surface of the collection cylinder 1. When the moving plate 16 slides, it will squeeze the return spring 20. When the return spring 20 is squeezed, it will contract downward. When the squeezing of the force-bearing inclined plate 17 by the squeezing bent rod 14 ends, at this time, the moving plate 16 has no squeezing force and will return to its original position through the elastic force of the return spring 20. At the same time, the driven push rod 18 will pull the contact telescopic rod 19 back to its original position. Since the transmission shaft 11 will drive the threaded ring 12 to keep rotating, and at the same time, the rotating ring 13 will drive the squeezing bent rod 14 to keep rotating and squeeze the force-bearing inclined plate 17. Since several squeezing bent rods 14 are provided and there is a certain distance between each squeezing bent rod 14, at this time, the force-bearing inclined plate 17 will be squeezed, so that the force-bearing inclined plate 17 will push the moving plate 16 to slide reciprocally. When the moving plate 16 slides reciprocally, it will push the driven push rod 18 to move reciprocally. At the same time, the driven push rod 18 will push the contact telescopic rod 19 to extend reciprocally and slightly impact the surface of the collection cylinder 1, so that the inner wall of the collection cylinder 1 generates a vibration feeling. When the transmission shaft 11 rotates, it will drive the rotating shaft 31 to rotate. When the rotating shaft 31 rotates, it will drive the driven ring 32 to rotate. When the driven ring 32 rotates, it will drive the rotating rod 33 to rotate. When the rotating rod 33 rotates, it will drive the cleaning plate 34 to rotate. When the cleaning plate 34 rotates, it will drive the cleaning brush 35 to rotate, so as to clean the inner wall of the collection cylinder 1 and save labor and improve the cleaning efficiency. When the bottom plate 3 rotates, it will drive the scraping plate 36 to rotate. When the scraping plate 36 rotates, it will scrape the inner wall of the collection cylinder 1. When the scraping plate 36 rotates, due to the inertia of rotation, the protein remaining on the inner wall of the collection cylinder 1 will be scraped to the inner wall of the guiding groove. When the protein flows into the guiding groove, it will flow downward along the guiding groove and cooperate with the vibration feeling generated by the impact of the contact telescopic rod 19 on the surface of the collection cylinder 1, so that the protein on the inner wall of the guiding groove will flow downward quickly, so as to quickly collect the protein on the inner wall of the collection cylinder 1. When the rotating shaft 31 rotates, it will drive the threaded rod 51 to rotate. When the threaded rod 51 rotates, it will make the threaded plate 52 move downward. When the threaded plate 52 moves, it will pull the scraping ring 53 downward. When the scraping ring 53 moves, it will scrape the lower surface of the inner wall of the collection cylinder 1 downward, scrape the protein on the inner wall downward, and make the protein flow downward through the guidance of the drainage inclined plate 58. At this time, the threaded plate 52 moves to the position where there is no thread on the surface of the threaded rod 51, and the threaded plate 52 is limited by the limit spring 56 to keep static. At this time, the threaded rod 51 keeps rotating.Meanwhile, the scraping ring 53 moves above the drainage inclined plate 58 and remains stationary without contacting the drainage inclined plate 58. When the scraping ring 53 moves downward, it will push the push plate 54 downward. When the push plate 54 moves, it will push the collection shrinkage ring 55 to shrink in the direction of approaching each other.,

[0030] The preferred embodiments of the present invention disclosed above are only used to help illustrate the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the present invention to the specific embodiments described. Obviously, many modifications and changes can be made according to the content of this specification. These embodiments are selected and specifically described in this specification to better explain the principles and practical applications of the present invention, so that those skilled in the relevant art can understand and utilize the present invention well. The present invention is only limited by the claims and their full scope and equivalents.

Claims

1. A protein collection device for stem cell differentiation and secretion based on the precipitation method, comprising a collection cylinder (1), a support foot (2) fixedly connected to the surface of the collection cylinder (1), a bottom plate (3) fixedly connected to the bottom of the support foot (2), a support frame (4) fixedly connected to the surface of the bottom plate (3), and a motor (5) fixedly connected to the top of the support frame (4), characterized in that, Further included; A vibration mechanism (10), the vibration mechanism (10) includes a moving plate (16), a driven push rod (18) is rotatably connected to the surface of the moving plate (16), and a contact telescopic rod (19) is rotatably connected to one end of the driven push rod (18) away from the moving plate (16); A scraping mechanism (30), the scraping mechanism (30) includes a scraping plate (36), a guiding groove is formed on the surface of the scraping plate (36), and a diversion groove plate (37) is fixedly connected to the bottom of the scraping plate (36); A collection mechanism (50), the collection mechanism (50) includes a threaded plate (52), scraping rings (53) are fixedly connected to both ends of the threaded plate (52), and a pushing plate (54) is rotatably connected to the bottom of the threaded plate (52).

2. The protein collection device for secretion produced by stem cell differentiation based on the precipitation method according to claim 1, wherein: The number of the support frames (4) is set to four, and the four support frames (4) are symmetrically arranged with the bottom plate (3) as the center. A receiving hole is formed in the bottom of the inner wall of the collection cylinder (1), and the number of the support feet (2) is set to three.

3. The protein collection device for the secretion produced by the differentiation of stem cells based on the precipitation method according to claim 2, wherein: The vibration mechanism (10) includes a transmission shaft (11), a threaded ring (12) is fixedly connected to the surface of the transmission shaft (11), a rotating ring (13) is fixedly connected to the surface of the threaded ring (12), an extrusion bent rod (14) is fixedly connected to the bottom of the rotating ring (13), a chute plate (15) is fixedly connected to the inner wall of the support frame (4), a force-receiving inclined plate (17) is fixedly connected to the top of the moving plate (16), and a return spring (20) is fixedly connected to the bottom of the moving plate (16).

4. A protein collection device for secreted proteins generated by stem cell differentiation based on the precipitation method according to claim 3, characterized in that: The end of the transmission shaft (11) is fixedly connected to the output end of the motor (5). Both ends of the moving plate (16) are slidably connected to the inner wall of the chute plate (15). One end of the extrusion bent rod (14) away from the rotating ring (13) contacts the surface of the force-receiving inclined plate (17). One end of the contact telescopic rod (19) away from the driven push rod (18) is fixedly connected to the inner wall of the support frame (4).

5. A protein collection device for secreting proteins generated by stem cell differentiation based on the precipitation method according to claim 4, characterized in that: The scraping mechanism (30) includes a rotating shaft (31), a driven ring (32) is fixedly connected to the surface of the rotating shaft (31), a rotating rod (33) is fixedly connected to the surface of the driven ring (32), a cleaning plate (34) is fixedly connected to one end of the rotating rod (33) away from the driven ring (32), and a cleaning brush (35) is fixedly connected to the surface of the cleaning plate (34).

6. The protein collection device produced by stem cell differentiation based on the precipitation method according to claim 5, wherein: The end of the rotating shaft (31) is fixedly connected to the end of the transmission shaft (11). The surface of the scraping plate (36) is fixedly connected to the surface of the rotating rod (33). One side of the scraping plate (36) away from the rotating rod (33) contacts the inner wall of the collection cylinder (1). One end of the cleaning brush (35) away from the cleaning plate (34) contacts the inner wall of the collection cylinder (1).

7. A protein collection device for the secretion produced by the differentiation of stem cells based on the precipitation method according to claim 6, wherein: The collection mechanism (50) includes a threaded rod (51), a collection contraction ring (55) is rotatably connected to one end of the pushing plate (54) away from the threaded plate (52), a limiting spring (56) is fixedly connected to the bottom of the threaded plate (52), a storage box (57) is arranged at the bottom of the collection cylinder (1), and a drainage inclined plate (58) is fixedly connected to the inner wall of the collection cylinder (1).

8. A protein collection device for secreted proteins generated by stem cell differentiation based on the precipitation method according to claim 7, characterized in that: One end of the rotating shaft (31) far from the transmission shaft (11) is fixedly connected to the end of the threaded rod (51). The bottom of the collection and contraction ring (55) contacts the bottom of the inner wall of the collection cylinder (1). The outer wall of the scraping ring (53) contacts the inner wall of the collection cylinder (1). One end of the threaded rod (51) far from the rotating shaft (31) is rotatably connected to the inner wall of the collection cylinder (1). The top of the storage box (57) contacts the bottom of the collection cylinder (1). The bottom of the storage box (57) contacts the surface of the bottom plate (3).

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