Test tube disinfection device for plant tissue culture

Abstract

The invention discloses a test tube disinfection device for plant tissue culture in the technical field of plant tissue culture. The test tube disinfection device comprises a shell and a test tube, a control panel is arranged on the surface of the shell, a recovery assembly for recovering and cleaning waste liquid is arranged on one side of the shell, a cleaning cavity is formed in the top of the shell, and a plurality of cleaning assemblies are arranged in the cleaning cavity; multiple heating assemblies are arranged on the inner wall of the cleaning cavity. Each cleaning assembly comprises an annular fixing groove formed in the inner bottom wall of the cleaning cavity, a moving rod in sliding fit with the shell is arranged in the center of each fixing groove, the top end of each moving rod is fixedly connected with an arc face coping, an extrusion cavity formed in the shell is formed under each moving rod, and a spring is arranged in each extrusion cavity. One end of each spring is fixedly connected with the bottom of the moving rod, and the other end is fixedly connected with the bottom end of the extrusion cavity. Through cooperation of the moving rod, the cleaning assembly and the heating assembly, the test tube cleaning device adapts to test tubes with different culture medium residues, and the test tubes are cleaned and disinfected by spraying hot water and high-temperature gas.

Description

Technical Field

[0001] The present invention relates to the technical field of plant tissue culture, and particularly relates to a test tube disinfection device for plant tissue culture. Background Art

[0002] Plant tissue culture, that is, plant tissue culture, refers to the technology of culturing the isolated tissues, organs or cells of plants on a culture medium containing various nutrients and plant hormones under aseptic conditions, so that they grow, differentiate and develop into complete plants.

[0003] In the prior art, generally after the experiment, for the test tubes used in plant tissue culture, the following steps can be taken: 1. Clean the contents of the test tubes (cleaning the culture medium and treating the plant materials); 2. Wash the test tubes; 3. Disinfect the test tubes (physical disinfection method and chemical disinfection method); 4. Store the test tubes and store them properly. Among them, the test tube washing stage specifically includes:

[0004] Initial rinsing: Rinse the test tubes with the contents cleared with tap water initially to remove most of the residual substances adhering to the inner wall of the test tubes.

[0005] Soaking treatment: Soak the test tubes in water containing an appropriate amount of dishwashing liquid or special glassware cleaning agent for a period of time (generally 15 - 30 minutes) to better remove stubborn stains and impurities.

[0006] Scrubbing and rinsing: Use a suitable test tube brush to gently scrub the inner wall of the test tubes to ensure that the stains on the test tube walls are completely removed. After scrubbing, rinse the test tubes repeatedly with tap water until the rinsing water is clear and foam-free. Finally, rinse with distilled water or deionized water 2 - 3 times to remove the minerals and other impurities that may remain in the tap water.

[0007] For different plant tissue culture experiments, the proportions of the culture media used are different, so there are differences in the difficulty of cleaning operations during subsequent cleaning and disinfection. When the amount of the culture media is large, for some existing cleaning and disinfection equipment, such as the patent with the authorization publication number CN114192528A (disclosing a test tube cleaning and disinfection device for biotechnological research and development, including a housing and a collection box, the collection box is arranged at the lower part of the housing; a support assembly, the support assembly is installed at the upper part inside the housing; a mounting frame, the mounting frame is slidably arranged at the lower part inside the housing; cleaning rods, the cleaning rods are rotatably installed at equal intervals on the mounting frame; water spraying pipes, the water spraying pipes are connected at equal intervals on the outer sides of the cleaning rods; a water spraying assembly, the water spraying assembly is installed at the bottom of the mounting frame; a cleaning assembly, the cleaning assembly is installed at the bottom of the mounting frame; a lifting assembly, the lifting assembly is installed on one side of the housing), it is necessary to insert the test tubes upside down on the cleaning rods and perform cleaning and disinfection by rotating the cleaning rods and spraying the cleaning liquid. However, if there is too much residual culture media in the test tubes, it will affect the cleaning rods from extending into the test tubes, so it is necessary to manually clean the culture media first and then put them into the equipment for cleaning and disinfection, which will also increase the manual workload. At the same time, since the residual culture media will adhere to the test tubes, and these cleaning rods usually adopt materials with good softness (to avoid damaging the test tubes during cleaning), it is also easy for the cleaning rods to fail to clean some small-volume culture media in place.

[0008] Therefore, the present invention proposes a test tube disinfection device for plant tissue culture to solve the above problems. Summary of the Invention

[0009] To solve the above problems, the present invention provides a test tube disinfection device for plant tissue culture, which dissolves the culture media in water by heating the cleaning liquid and high-temperature gas, and quickly completes cleaning and disinfection.

[0010] To achieve the above purpose, the technical solution of the present invention is as follows: A test tube disinfection device for plant tissue culture includes a housing and test tubes. A control panel is arranged on the surface of the housing. A recovery assembly for recovering the cleaning waste liquid is arranged on one side of the housing. A cleaning cavity is opened at the top of the housing, and a number of cleaning components for cleaning and disinfecting the test tubes are arranged in the cleaning cavity; A storage box for storing distilled water is arranged on one side of the housing; A number of heating components for heating the gas inside the cleaning cavity and heating the distilled water are arranged on the inner wall of the cleaning cavity;

[0011] The cleaning components all include an annular fixing groove opened on the bottom wall of the cleaning cavity. A moving rod that is slidably engaged with the housing is provided at the center position of each fixing groove. An arc-shaped pressing top is fixedly connected to the top end of each moving rod. An extrusion cavity opened inside the housing is provided directly below each moving rod. The bottom end of each moving rod extends into the corresponding extrusion cavity and is slidably engaged with it. A spring is provided in each extrusion cavity. One end of each spring is fixedly connected to the bottom of the moving rod, and the other end of each spring is fixedly connected to the bottom wall of the extrusion cavity. A pump assembly for conveying distilled water and gas to the top end of the arc-shaped pressing top is provided inside each moving rod. A fixing assembly for fixing the test tube is provided on the inner circumferential wall of each fixing groove.

[0012] Principle of the basic solution: By directly placing the test tube upside down in the fixing groove, the culture medium in the test tube presses the arc-shaped pressing top. Then, start the heating component to heat the air in the cleaning cavity and simultaneously heat the distilled water input into the first pump assembly, so that the heated distilled water sprays out from the upper surface of the arc-shaped pressing top. Furthermore, due to the reaction force during the water flow spraying and the structure of the arc-shaped pressing top, a certain cavity is formed between the arc-shaped pressing top and the culture medium, enabling the culture medium to be quickly dissolved in water under the dual action of the hot water inside the test tube and the hot air outside the test tube, and flowing out along the periphery of the arc-shaped pressing top to clean the inner wall of the test tube. Then, the recovery component recovers the waste liquid after cleaning. After the culture medium is cleaned, hot air is introduced into the test tube to perform drying and dehumidification while further performing high-temperature disinfection.

[0013] The following beneficial effects are achieved by adopting the above solution:

[0014] 1. Compared with the prior art, this solution realizes the rapid dissolution of the culture medium residue and the synchronous cleaning of the inner wall of the test tube through the synergistic effect of the arc-shaped pressing top and the heating component. In the traditional method, the residual culture medium in the test tube often needs to be manually scrubbed repeatedly or soaked for a long time. However, this solution uses a dual heating mechanism of hot water spraying and external hot air to completely dissolve the solid culture medium (especially the solidified components containing agar) in a short time. The arc-shaped design of the arc-shaped pressing top makes the water flow radiate outward, forming an all-round scouring of the inner wall of the test tube, effectively removing the organic residues attached to the tube wall. At the same time, the reaction force of the water flow spraying forms a dynamic cavity between the arc-shaped pressing top and the culture medium, avoiding the problem that the residues are difficult to dissolve due to local accumulation. In addition, the high-temperature steam disinfection link after cleaning further ensures the sterile state of the test tube, reducing the possibility of secondary pollution risks that may occur in the traditional step-by-step operations (cleaning → drying → sterilization), and significantly improving the processing efficiency and safety.

[0015] 2. In this solution, the modular layout of the cleaning chamber, the recycling component, the storage box and the fixing groove realizes the full-process automation of test tube cleaning, waste liquid recycling, disinfection and drying. In traditional test tube cleaning, each test tube needs to be processed manually one by one, which is cumbersome and prone to test tube breakage due to improper operation. However, in this device, through the elastic cooperation between the fixing groove and the moving rod (spring buffer design), test tubes with different proportions of culture medium can be adapted, and the height can be automatically adjusted when the arc-shaped top is pressed, avoiding damage to the test tubes caused by mechanical hard contact. In addition, the shunt design of the first pump component and the second pump component enables seamless connection between the distilled water cleaning and gas drying processes, eliminating the need to replace equipment or adjust parameters, reducing manual intervention. The integrated design of the recycling component can also centrally process the waste liquid containing the culture medium, avoiding environmental pollution caused by direct discharge. The overall structure realizes one-key operation through the control panel, which not only lowers the operation threshold but also reduces the risk of equipment failure caused by human error, prolonging the service life of the device.

[0016] 3. Through the dynamic regulation of the heating component and the elastic adaptation mechanism of the fixing component, this solution significantly reduces the dependence on manual pretreatment and chemical cleaning agents, achieving efficient utilization of resources and environmental protection treatment. In the prior art, the residual solid culture medium needs to be manually scraped in advance or dissolved by relying on chemical solvents, which is not only time-consuming and laborious but also may cause the risk of secondary pollution due to the residue of cleaning agents. In this device, the arc-shaped structure of the arc-shaped top is combined with the adjustable moving rod, which can automatically adapt to the volume and distribution form of different residues when the test tube is inverted. When the amount of culture medium is large, an adaptive cavity is formed between the arc-shaped top and the culture medium, ensuring that the dual effects of hot water spraying and hot gas penetration fully cover the surface of the residue, and the dissolution of stubborn residues can be completed without manual intervention.

[0017] Furthermore, the recycling component includes a recycling groove opened on one side of the housing, and a waste liquid box is slidably fitted in the recycling groove.

[0018] Beneficial effects: The sliding fit design of the recycling groove and the waste liquid box realizes the efficient collection and rapid treatment of waste liquid. The waste liquid box can be conveniently pulled out for centralized cleaning or replacement, avoiding the risk of secondary pollution caused by the retention of waste liquid in traditional equipment.

[0019] Furthermore, each heating component includes an electric heater arranged on the inner wall of the cleaning chamber, and the electric heaters are all electrically connected to the control panel.

[0020] Beneficial effects: The electric heaters are integrated on the inner wall of the cleaning chamber and linked with the control panel to achieve precise temperature control and rapid response. The heating temperature and duration can be customized through the control panel to adapt to the dissolution requirements of different culture medium components (such as differences in agar content). The direct embedded layout of the electric heaters shortens the heat transfer path. Combined with the closed design of the cleaning chamber, the internal gas and distilled water are quickly heated, significantly improving the heating efficiency and thus enhancing the high-temperature disinfection efficiency.

[0021] Furthermore, the fixed components all include an airbag layer fixedly connected along the inner ring wall of the fixed groove, the airbag layer is connected to an inflation pipe and an exhaust pipe, one end of the inflation pipe is connected to the extrusion chamber, and a one-way valve is provided in the inflation pipe, one end of the exhaust pipe is connected to the cleaning chamber, and an exhaust valve electrically connected to the control panel is provided in the exhaust pipe.

[0022] Beneficial effects: The linkage design of the airbag layer and the filling / exhaust tube realizes flexible and adaptive fixation of the test tube and efficient gas source utilization. When the test tube is inserted upside down into the fixing groove, the moving rod is pressed down, and the gas in the extrusion cavity enters the inflation tube through the one-way valve, driving the airbag layer to expand and fit tightly to the wall of the test tube, and adaptive clamping can be completed without an additional air pump. The flexible contact of the airbag avoids the risk of scratches or ruptures of the test tube that may be caused by traditional rigid clamps. After cleaning is completed, the control panel triggers the exhaust valve to open, and the gas in the airbag is discharged into the cleaning chamber through the exhaust pipe, and simultaneously participates in the hot air circulation, which not only accelerates the drying of the test tube, but also avoids the energy waste caused by separate exhaust.

[0023] Furthermore, the pump assemblies include a water pump and an air pump arranged in the shell, the water pump output end is connected to a spray pipe, one end of the spray pipe extends along the inside of the moving rod to the upper surface of the arc pressure top, the water pump input end is connected to a water pipe, one end of the water pipe is connected to the storage box and the water pipes pass through the corresponding electric heater; the air pump output end is connected to an injection pipe, one end of the injection pipe extends along the inside of the moving rod to the upper surface of the arc pressure top, and the air pump input end is connected to the cleaning chamber; the water pump and the air pump are electrically connected to the control panel.

[0024] Beneficial effects: The independently controlled pump component system realizes efficient coordination of the cleaning and disinfection processes. The first pump component precisely heats the distilled water through the electric heater to ensure the optimal water temperature required for dissolving the culture medium. At the same time, the jet pipe delivers hot water in a directional manner to form a high-pressure flushing force; the second pump component uses the circulating hot air in the cleaning chamber as the air source, and outputs high-temperature airflow through the jet pipe to form a closed-loop thermal energy utilization. The dual-pump linkage can perform cleaning and drying simultaneously (such as hot water flushing followed by hot air drying), or it can operate independently in stages to adapt to different cleaning needs.

[0025] Furthermore, the surface of the airbag layer is provided with a plurality of grooves in a circular array; a recovery channel connected to the waste liquid box is opened at the bottom of the fixed groove; and the bottom of the cleaning cavity surrounded by the fixed groove is a boss structure.

[0026] Beneficial effects: The annular groove design on the surface of the airbag layer enhances the grasping force on the outer wall of the test tube and the air flow guiding ability. When the airbag is inflated, the grooves form local micro-convex structures, which improve the stability of test tube fixation through multi-point contact, preventing the test tube from sliding or shifting during the cleaning process. At the same time, the groove gaps can serve as air or liquid channels to ensure the normal flow of gas and liquid. The recovery channel at the bottom of the fixing groove is directly connected to the waste liquid box, ensuring the rapid centralized diversion of the dissolved culture medium waste liquid and rinsing water, avoiding waste liquid retention or dripping and polluting the inner cavity of the equipment. The convex platform structure at the bottom of the cleaning chamber guides the water flow along the edge of the convex platform into the recovery channel through the inclined surface, reducing liquid residue and further improving the cleaning thoroughness and equipment cleaning efficiency.

[0027] Furthermore, the gaps between the arc-shaped pressing top and the inner wall of the test tube are all 1 - 1.5 mm.

[0028] Beneficial effects: The gap design of 1 - 1.5 mm between the arc-shaped pressing top and the inner wall of the test tube achieves an efficient and non-damaging cleaning effect by precisely controlling the water flow pattern and coverage. This gap, combined with the curved surface guidance of the arc-shaped pressing top, enables the heated distilled water to form a continuous water curtain around the inner wall of the test tube along the gap after spraying, evenly covering the entire inner wall of the test tube, thoroughly flushing the attached and residual culture medium, and avoiding local cleaning blind spots caused by pressure concentration in traditional direct spray water flows. The laminar flow characteristics of the water curtain can not only fully dissolve gelling substances such as agar but also reduce the mechanical friction on the inner wall of the test tube through flexible flushing, especially suitable for test tubes made of thin-walled or easily scratched materials. At the same time, the water curtain and the external hot air act synergistically to form a "liquid + gas" two-phase heat transfer, accelerating the heat penetration to the deep layer of the residue and significantly shortening the dissolution time.

[0029] Furthermore, the diameter of the injection tube near the arc-shaped pressing top is larger than the diameter of the air injection tube near the arc-shaped pressing top.

[0030] Beneficial effects: The differential pipe diameter design of the injection tube and the air injection tube realizes the dynamic coordination between the cleaning and drying stages through the regulation of the reaction force. The air injection tube has a smaller diameter, so the gas velocity and reaction force are higher when the gas is ejected, driving the moving rod to quickly move down against the spring resistance, forming a larger cavity between the arc-shaped pressing top and the culture medium residue, providing sufficient space for the rapid injection and circulation of the subsequent hot air. The injection tube has a larger diameter, which reduces the reaction force while ensuring the water flow coverage area, preventing the moving rod from moving down excessively and causing the attenuation of the water curtain pressure. This design preferentially uses the water flow to wash and dissolve the culture medium during the cleaning stage, and then triggers the displacement of the moving rod through the high-speed airflow of the air injection tube, enabling the high-temperature hot air to quickly fill the cavity formed by the electrodes and directly act on the inner wall of the test tube and the deep layer of the residue, accelerating the water evaporation and sterilization process.

[0031] Furthermore, a number of rubber sheets are provided inside both the injection tube and the air injection tube near the arc-shaped pressing top.

[0032] Beneficial effects: The flexible sealing design of the rubber sheet effectively prevents the backflow of liquids or gases. During the spraying process, the water flow or air flow pushes the rubber sheet to expand outward, forming a directional spraying channel to ensure that the medium accurately covers the inner wall of the test tube; when the spraying stops, the rubber sheet naturally closes to block the reverse intrusion of external pollutants into the pipeline.

[0033] Furthermore, a sealing cover is hinged to the top of the housing.

[0034] Beneficial effects: The hinged sealing cover can be quickly opened and closed to ensure the airtightness of the cleaning chamber, prevent the leakage of high-temperature steam and the intrusion of external pollutants, which not only ensures operation safety but also maintains the stability of the internal thermal environment, improving the cleaning and disinfection efficiency.

[0035] The additional aspects and advantages of the present invention will be partly given in the following description, partly will become obvious from the following description, or will be understood through the practice of the present invention. Description of the Drawings

[0036] Figure 1 is the overall axonometric view of the embodiment of the test tube disinfection device for plant tissue culture of the present invention;

[0037] Figure 2 is the overall right side view of the embodiment of the test tube disinfection device for plant tissue culture of the present invention;

[0038] Figure 3 is the front sectional view of the cleaning component of the embodiment of the test tube disinfection device for plant tissue culture of the present invention;

[0039] Figure 4 is the enlarged view of part A of the embodiment of the test tube disinfection device for plant tissue culture of the present invention;

[0040] Figure 5 is the enlarged view of part B of the embodiment of the test tube disinfection device for plant tissue culture of the present invention.

[0041] The reference numerals in the drawings of the specification include: 1, housing; 2, waste liquid box; 3, cleaning chamber; 4, electric heater; 5, sealing cover; 6, test tube; 7, control panel; 8, fixing groove; 9, solid medium; 10, arc-shaped pressing top; 11, moving rod; 12, spring; 13, air pump; 14, water pump; 15, spraying tube; 16, air spraying tube; 17, recovery channel. Detailed Embodiments

[0042] The technical solutions of the present invention will be clearly and completely described below with reference to the drawings. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of them. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts belong to the scope of protection of the present invention.

[0043] In the description of the present invention, it should be noted that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus cannot be construed as a limitation on the present invention. In addition, the terms "first", "second", and "third" are only used for descriptive purposes and cannot be construed as indicating or implying relative importance.

[0044] In the description of the present invention, it should be noted that unless otherwise clearly specified and defined, the terms "installed", "connected", and "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0045] The following is a further detailed description through specific embodiments:

[0046] Embodiment 1:

[0047] As shown in Figure 1 , Figure 2 , Figure 3 , Figure 4 and Figure 5 : A test tube disinfection device for plant tissue culture, comprising a housing 1 and a test tube 6. A control panel 7 is provided on the surface of the housing 1. A sealing cover 5 is hinged to the top of the housing 1 for heat preservation inside the housing 1 and protecting personnel safety. A recovery component for recovering and cleaning waste liquid is provided on one side of the housing 1. A cleaning cavity 3 is opened at the top of the housing 1. A number of cleaning components for cleaning and disinfecting the test tube 6 are provided inside the cleaning cavity 3; A storage tank for storing distilled water is provided on one side of the housing 1; A number of heating components for heating the gas inside the cleaning cavity 3 and heating the distilled water are provided on the inner wall of the cleaning cavity 3.

[0048] The cleaning components all include an annular fixing groove 8 opened on the inner bottom wall of the cleaning chamber 3. At the central position of the fixing groove 8, a moving rod 11 slidably fitted with the housing 1 is provided. At the top of the moving rod 11, an arc-shaped pressing top 10 is fixedly connected. Below the moving rod 11, an extrusion chamber opened inside the housing 1 is provided. The bottom ends of the moving rods 11 all extend into the corresponding extrusion chambers and are slidably fitted with them. Springs 12 are arranged in the extrusion chambers. One end of each spring 12 is welded to the bottom of the moving rod 11, and the other end of each spring 12 is welded to the inner bottom wall of the extrusion chamber; a pump assembly for conveying distilled water and gas to the top of the arc-shaped pressing top 10 is arranged inside each moving rod 11; fixing components for fixing the test tubes 6 are arranged on the inner ring wall of the fixing groove 8.

[0049] The recycling component includes a recycling groove opened on one side of the housing 1, and a waste liquid box 2 is slidably fitted in the recycling groove.

[0050] The heating components all include electric heaters 4 arranged on the inner wall of the cleaning chamber 3, and the electric heaters 4 are all electrically connected to the control panel 7.

[0051] The fixing components all include an airbag layer fixedly connected along the inner ring wall of the fixing groove 8. The airbag layers are all communicated with an inflation pipe and an exhaust pipe. One end of the inflation pipe is communicated into the extrusion chamber, and a one-way valve is arranged in the inflation pipe. One end of the exhaust pipe is communicated into the cleaning chamber 3, and an exhaust valve electrically connected to the control panel 7 is arranged in the exhaust pipe.

[0052] A number of grooves are annularly and arrayedly arranged on the surface of the airbag layer; a recycling channel 17 communicated to the waste liquid box 2 is opened at the bottom of the fixing groove 8; the bottom of the cleaning chamber 3 surrounded by the fixing groove 8 is a convex platform structure. The gap between the arc-shaped pressing top 10 and the inner wall of the test tube 6 is 1 - 1.5 mm.

[0053] The pump assemblies all include a water pump 14 and an air pump 13 arranged in the housing 1. The output end of the water pump 14 is communicated with a spray pipe 15. One end of the spray pipe 15 extends along the inside of the moving rod 11 to the upper surface of the arc-shaped pressing top 10. The input end of the water pump 14 is communicated with a water pipe. One end of the water pipe is communicated into the storage tank and the water pipes all pass through the corresponding electric heaters 4, and can be heated to a set temperature (adjustable from 50 - 90 °C) synchronously when conveying distilled water; the output end of the air pump 13 is communicated with an air spray pipe 16. One end of the air spray pipe 16 extends along the inside of the moving rod 11 to the upper surface of the arc-shaped pressing top 10. The input ends of the air pumps 13 are all communicated into the cleaning chamber 3, and can extract preheated air (temperature 60 - 120 °C) by the electric heaters 4; the water pump 14 and the air pumps are all electrically connected to the control panel 7; a number of rubber sheets are arranged inside one end of the spray pipe 15 and the air spray pipe 16 close to the arc-shaped pressing top 10.

[0054] The control panel 7 presets a "cleaning - drying" linkage program:

[0055] Cleaning stage: Start the water pump 14. The heated distilled water (80 °C) flushes the inner wall of the test tube 6 through the spray pipe 15 for 10 minutes to dissolve the agar gel.

[0056] Transition stage: The power of the water pump 14 is reduced to 0 - 20%, and the air pump 13 is started synchronously. The residual heat in the cleaning chamber 3 is used to preliminarily dry the test tube 6.

[0057] Drying stage: Turn off the water pump 14, and the air pump 13 operates at full power. The hot air pipe 16 outputs hot air at 100 °C for 5 minutes to complete sterilization.

[0058] During this process, the air extracted by the air pump 13 from the cleaning chamber 3 at the input end has been reheated by the electric heater 4, forming a closed-loop utilization of thermal energy.

[0059] On the one hand, the culture medium used in most plant tissue culture experiments is solid. By adding 0.6 - 1% agar as a coagulant, a gel-like support structure is formed. The solid culture medium 9 is convenient for fixing explants and is suitable for organ differentiation (such as bud and root induction) or callus culture. Therefore, the solid culture medium 9 is used as an example for illustration as follows:

[0060] Invert and insert the plant tissue culture test tube 6 with the solid culture medium 9 after use into the fixing groove 8. When the test tube 6 is pressed down, the solid culture medium 9 inside it contacts the arc-shaped top 10 and pushes the moving rod 11 to slide downward. The bottom end of the moving rod 11 compresses the spring 12 in the extrusion cavity. At the same time, the air pressure generated by the downward movement of the moving rod 11 presses the gas in the extrusion cavity into the airbag layer through the air filling pipe (a one-way valve is set inside the air filling pipe to only allow gas to flow in one direction). The airbag layer inflates and the surface grooves fit tightly with the outer wall of the test tube 6.

[0061] Set parameters (such as water temperature of 80°C and heating duration of 5 minutes) through the control panel 7, and start the electric heater 4 to heat the gas inside the cleaning chamber 3 and the distilled water in the storage tank simultaneously. The water pump 14 transports the heated distilled water to the spray pipe 15 through the water pipe. The hot water is sprayed out from the surface of the arc-shaped top 10 and fills the cavity between the culture medium and the arc-shaped top 10, dissolving the culture medium in the hot water. Then, it flows along the inner wall of the test tube 6 in the form of an annular water curtain (the gap between the arc-shaped top 10 and the inner wall of the test tube 6 is 1 - 1.5 mm), covering the entire circumference of the inner wall of the test tube 6. The dual action of the continuous scouring of the water curtain and the external hot air (the temperature of the cleaning chamber 3 is maintained at 60°C) enables the agar in the solid culture medium 9 to dissolve quickly, and the residual organic matter flows into the waste liquid box 2 along the recovery channel 17 at the bottom of the fixed groove 8 with the water flow. At the same time, in order to enhance the disinfection effect, disinfectant can be added to the distilled water. As the solid culture medium 9 gradually dissolves in the hot water, since the flow rate of the liquid ejected from the spray pipe 15 is constant, in order to maintain the mechanical balance of the moving rod 11, the moving rod 11 will adaptively move upward under the action of the spring 12 until the solid culture medium 9 in the test tube 6 is completely dissolved and cleaned. For the switching of the water / gas spraying process, a distance measuring sensor can be set at the bottom of the moving rod 11. After the moving rod 11 moves upward to the initial position and stays for a certain period of time (preset buffer time), high-temperature gas disinfection and drying are carried out.

[0062] After the cleaning of the culture medium is completed, the control panel 7 switches to the operation of the air pump 13. The air pump 13 extracts the high-temperature gas (about 100°C) inside the cleaning chamber 3 and sprays it out from the surface of the arc-shaped top 10 at high speed through the air spray pipe 16. Since the diameter of the air spray pipe 16 is smaller than that of the spray pipe 15, the reaction force of the gas spraying further pushes the moving rod 11 downward, expanding the gap between the arc-shaped top 10 and the bottom of the test tube 6, enabling the hot air to quickly fill the inner cavity of the test tube 6, accelerating the evaporation of water, and further performing high-temperature disinfection. The high-temperature gas acts continuously for 10 minutes to achieve the drying and sterilization of the inner wall of the test tube 6.

[0063] After the drying of the test tube 6 is completed, the exhaust valve is opened, and the gas inside the airbag layer is discharged into the cleaning chamber 3 through the exhaust pipe, releasing the restriction on the test tube 6, which is convenient for the staff to take it after its temperature drops to room temperature.

[0064] Thus, even if a large amount of solid culture medium 9 remains in the test tube 6, the cavity formed after the arc-shaped top 10 is pressed can still allow hot water to penetrate and dissolve it, without the need for manual pre-scraping.

[0065] On the other hand, for some liquid media or semi-solid / semi-liquid media, since the arc-shaped top 10 cannot be extruded, after these test tubes 6 are inverted and fixed, the inflation mode is manually started through the control panel 7 (an air pump 13 connected to the airbag layer is additionally provided). The air pump 13 actively inflates the airbag layer (bypassing the air pressure triggering mechanism of the moving rod 11), causing the airbag to expand and fit against the outer wall of the test tube 6 to complete the fixation. The inflation pressure is preset by the control panel 7 (such as 0.05 - 0.1 MPa) to prevent the test tube 6 from deforming due to excessive air pressure.

[0066] The sugars or organic components in the liquid medium are easily soluble in water and do not require high-temperature dissolution. The control panel 7 sets the water temperature at 40 - 50 °C, and the water pump 14 is started to jet from the arc-shaped top 10 with a high-pressure water flow (0.3 - 0.5 MPa) to directly wash the inner wall of the test tube 6. The water flow forms an annular water curtain through a 1 - 1.5 mm gap, covering the entire tube wall and effectively removing the viscous residues. After the cleaning is completed, the air pump 13 and the electric heater 4 are started synchronously to extract air from the cleaning chamber 3, heat it to 100 - 120 °C through the heater, and then inject it into the inner cavity of the test tube 6 through the air injection pipe 16 to accelerate water evaporation and high-temperature disinfection.

[0067] Moreover, when the waste liquid of the liquid medium flows into the waste liquid box 2 through the recovery channel 17, it is prone to breed microorganisms due to its high sugar content. The device presets an ultraviolet sterilization module (ultraviolet lamp) in the waste liquid box 2, and the control panel 7 can independently control its start and stop to perform real-time sterilization treatment on the waste liquid and prevent the spread of biological contamination.

[0068] Example 2:

[0069] The difference from Example 1 is that, as Figure 4 shown, the diameter of the end of the injection tube 15 close to the arc-shaped top 10 is larger than the diameter of the end of the air injection pipe 16 close to the arc-shaped top 10. For example, the diameter of the end of the injection tube 15 is 1.5 mm, and the diameter of the end of the air injection pipe 16 is 0.5 mm. This design strengthens the dynamic synergistic effect in the cleaning and drying stages through hydrodynamic differences:

[0070] Cleaning stage: The larger diameter of the injection tube 15 reduces the reaction force of the water flow jet, keeping the moving rod 11 stable under the support of the spring 12, and maintaining a 1.5 - 2.5 mm gap between the arc-shaped top 10 and the medium to ensure that the hot water forms a continuous water curtain and fully dissolves the medium;

[0071] Drying stage: Since the diameter of the air jet pipe 16 is smaller than that of the injection pipe 15, the smaller pipe diameter of the air jet pipe 16 increases the gas jet speed to 5 m / s, and the reaction force increases significantly, pushing the moving rod 11 to move down 2-3 mm. The arc surface pressing top 10 and the bottom of the test tube 6 form an enlarged cavity (the gap increases to 3.5-5 mm), so that the hot air quickly fills the inner cavity of the test tube 6. The high-temperature gas acts continuously for 10 minutes to achieve the drying and sterilization of the inner wall of the test tube 6 and improve the drying and disinfection efficiency.

[0072] The differential pipe diameter design dynamically adjusts the cavity volume through the reaction force, realizes the precise switching between "large water curtain cleaning" and "strong air flow drying", not only ensures the thorough dissolution of the solid medium 9, but also accelerates the heat energy penetration through the air flow expansion. It is especially suitable for the treatment of stubborn residues of high-viscosity media (such as those containing 1.2% agar). At the same time, this structure does not require an additional driving device and only realizes mechanical self-adaptation through hydrodynamic characteristics, reducing the equipment complexity and maintenance cost.

[0073] Obviously, the above embodiments are only examples given for clear illustration and are not limitations on the implementation manners. For those of ordinary skill in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation manners here. The obvious changes or modifications derived therefrom are still within the protection scope of the present invention.

Claims

1. A test tube disinfection device for plant tissue culture, comprising a housing (1) and a test tube (6). A control panel (7) is arranged on the surface of the housing (1), and it is characterized in that, On one side of the housing (1), there is a recycling component for recycling and cleaning waste liquid. At the top of the housing (1), there is a cleaning chamber (3), and several cleaning components for cleaning and disinfecting test tubes (6) are arranged in the cleaning chamber (3); on one side of the housing (1), there is a storage tank for storing distilled water; on the inner wall of the cleaning chamber (3), there are several heating components for heating the gas inside the cleaning chamber (3) and heating the distilled water. Each cleaning component includes an annular fixing groove (8) opened on the inner bottom wall of the cleaning chamber (3). At the central position of each fixing groove (8), there is a moving rod (11) slidably matched with the housing (1). At the top of each moving rod (11), there is a fixed arc-shaped pressing top (10). Just below each moving rod (11), there is an extrusion chamber opened inside the housing (1). The bottom ends of the moving rods (11) all extend into the corresponding extrusion chambers and are slidably matched with them. Inside each extrusion chamber, there is a spring (12). One end of each spring (12) is fixedly connected to the bottom of the moving rod (11), and the other end of each spring (12) is fixedly connected to the inner bottom wall of the extrusion chamber; inside each moving rod (11), there is a pump component for conveying distilled water to the top of the arc-shaped pressing top (10); on the inner circumferential wall of each fixing groove (8), there is a fixing component for fixing the test tube (6).

2. The test tube disinfection device for plant tissue culture according to claim 1, characterized in that: The recycling component includes a recycling groove opened on one side of the housing (1), and a waste liquid box (2) is slidably matched inside the recycling groove.

3. The test tube disinfection device for plant tissue culture according to claim 2, characterized in that: Each heating component includes an electric heater (4) arranged on the inner wall of the cleaning chamber (3), and each electric heater (4) is electrically connected to the control panel (7).

4. The test tube disinfection device for plant tissue culture according to claim 3, characterized in that: Each fixing component includes an airbag layer fixedly connected along the inner circumferential wall of the fixing groove (8). Each airbag layer is communicated with an inflation pipe and an exhaust pipe. One end of each inflation pipe is communicated to the inside of the extrusion chamber, and a one-way valve is arranged inside each inflation pipe. One end of each exhaust pipe is communicated to the inside of the cleaning chamber (3), and an exhaust valve electrically connected to the control panel (7) is arranged inside each exhaust pipe.

5. The test tube disinfection device for plant tissue culture according to claim 4, characterized in that: Each pump component includes a water pump (14) and an air pump (13) arranged inside the housing (1). The output ends of the water pumps (14) are all communicated with a spray pipe (15). One end of the spray pipe (15) extends along the inside of the moving rod (11) to the upper surface of the arc-shaped pressing top (10). The input end of the water pump (14) is communicated with a water pipe. One end of the water pipe is communicated to the inside of the storage tank and the water pipes all pass through the corresponding electric heaters (4); the output ends of the air pumps (13) are all communicated with an air spray pipe (16). One end of the air spray pipe (16) extends along the inside of the moving rod (11) to the upper surface of the arc-shaped pressing top (10). The input ends of the air pumps (13) are all communicated to the inside of the cleaning chamber (3); the water pumps (14) and the air pumps (13) are all electrically connected to the control panel (7).

6. The test tube disinfection device for plant tissue culture according to claim 5, characterized in that: Several grooves are annularly and arrayedly arranged on the surface of each airbag layer; a recycling channel (17) communicated to the waste liquid box (2) is opened at the bottom of the fixing groove (8); the bottom of the cleaning chamber (3) surrounded by the fixing groove (8) is a convex platform structure.

7. The test tube disinfection device for plant tissue culture according to claim 6, characterized in that: The gap between the arc-shaped pressing top (10) and the inner wall of the test tube (6) is 1 - 1.5 mm.

8. The test tube disinfection device for plant tissue culture according to claim 7, wherein: The diameter of the end of the spray pipe (15) close to the arc-shaped pressing top (10) is larger than the diameter of the end of the air spray pipe (16) close to the arc-shaped pressing top (10).

9. The test tube disinfection device for plant tissue culture according to claim 8, characterized in that: Inside the injection pipe (15) and the air injection pipe (16) near one end close to the arc-shaped top pressing (10), a number of rubber sheets are provided.

10. The test tube disinfection device for plant tissue culture according to claim 9, characterized in that, A sealing cover (5) is hinged to the top of the housing (1).

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