Chinese mesona herb hydroponic seedling culture nutrient solution

Abstract

The invention belongs to the technical field of Chinese mesona herb hydroponic culture, and particularly relates to a Chinese mesona herb hydroponic seedling culture nutrient solution. The invention relates to a Chinese mesona herb hydroponic seedling culture nutrient solution which is based on a 1 / 2 Hoagland nutrient solution and is added with alpha-naphthylacetic acid, oryzanol and brassinolide, the final concentration of the alpha-naphthylacetic acid is 0.3 mg / L to 0.7 mg / L, the final concentration of the oryzanol is 0.3 mg / L to 0.7 mg / L, and the final concentration of the brassinolide is 0.01 mg / L. According to the Chinese mesona herb hydroponic nutrient solution, the 1 / 2 Hoagland nutrient solution serves as the basis, the alpha-naphthylacetic acid, the oryzanol and the brassinolide are added at the same time, when the hydroponic nutrient solution is used for cultivating Chinese mesona herb cutting slips, the rooting rate, the average rooting number and the morbidity can reach 95%, 7.8 pieces and 19% respectively, and the rooting rate, the average rooting number and the morbidity can reach 95%, 7.8 pieces and 19% respectively. The alpha-naphthylacetic acid, the oryzanol and the brassinolide which are added into the nutrient solution have a synergistic effect, so that the rooting and disease-resistant effects of the mesona chinensis cutting slips can be improved, and support can be provided for hydroponic seedling culture of the mesona chinensis.

Description

Technical Field

[0001] The invention belongs to the technical field of hydroponic culture of agar-agar grass, and particularly relates to a nutrient solution for hydroponic culture of agar-agar grass seedlings. Background Art

[0002] Agar-agar is an annual herbaceous perennial plant of the genus Agar-agar of the family Lamiaceae. It is also known as fairy grass and is an important edible and medicinal crop. It can be used to make agar-agar and herbal tea, and can also be used in plant beverages such as cola. It has the effects of clearing away heat and thirst, removing heat and promoting diuresis. It can also be processed into medicines with health care functions, which have the effects of calming, cooling, lowering blood sugar and lowering blood pressure. At present, the breeding and cultivation of agar-agar mostly adopts soil culture methods such as layering propagation, cutting seedling cultivation, division propagation and tissue culture propagation. There are disadvantages such as fertilizer loss, pests and diseases, and continuous cropping obstacles. The hydroponic method can effectively overcome the shortcomings of the soil culture method for breeding agar-agar, and is less affected by the natural environment, and can also alleviate the problem of limited land resources. Therefore, it is of great significance to study the hydroponic seedlings of agar-agar.

[0003] In the prior art, there are few relevant literatures on the hydroponics of Agarwood. Only in "Determination of Polysaccharide and Flavonoid Content in Different Parts of Agarwood and Hydroponic Research" (Du Yujie et al., 2023) is the hydroponic research on Agarwood mentioned, which discloses that N6-furanmethyladenine KT, α-naphthylacetic acid NAA and indolebutyric acid IBA have relatively good rooting promotion effects on hydroponic Agarwood, but KT promotes finer roots, NAA promotes thicker and sparser roots, and IBA is moderate; the optimal concentration of each exogenous regulator to promote the rooting effect of hydroponic Agarwood is 0.5 mg / L, and the rooting effect decreases successively with increasing concentration; after a short period of negative weight growth in N6·MS nutrient solution of different concentrations, Agarwood shows different degrees of weight gain.

[0004] There are many reports on hydroponic cultivation of other plants, such as CN119707559A, which discloses a hydroponic nutrient solution containing growth regulators and a hydroponic method for grape cuttings, wherein the hydroponic nutrient solution includes the following mass concentration components: 1 / 2 Hogland 1.0-1.5g / L, sucrose 20-30g / L, indoleacetic acid 0.4-0.6g / L, indolebutyric acid 2.5-3.5g / L, naphthaleneacetic acid 0.8-1.2g / L and zeatin 1.8-2.2mg / L. Indoleacetic acid, indolebutyric acid, naphthaleneacetic acid and zeatin in the patented hydroponic nutrient solution can synergistically promote the rooting of grape cuttings, with a rooting rate of up to 98%.

[0005] The inventors used 1 / 2 Hoagland nutrient solution as the basis, and added naphthylacetic acid, oryzanthin and brassinolide at the same time, which not only promoted the rooting of the agarwood cuttings, but also improved the disease resistance of the agarwood cuttings. No relevant reports in this regard have been seen so far.

[0006] The information disclosed in this background section is only intended to enhance the overall understanding of the background of the present invention and should not be regarded as an admission or any form of implication that this information constitutes prior art already known to those of ordinary skill in the art. Summary of the Invention

[0007] The object of the present invention is to provide a nutrient solution for water-cultured seedlings of Mesona chinensis, which can promote the rooting of Mesona chinensis cuttings and improve the disease resistance of Mesona chinensis cuttings.

[0008] To achieve the above object, the present invention provides the following technical solutions:

[0009] A nutrient solution for water-cultured seedlings of Mesona chinensis, which is based on 1 / 2 Hoagland nutrient solution and added with α-naphthaleneacetic acid, gliotoxin and brassinolide; the final concentrations of the α-naphthaleneacetic acid, gliotoxin and brassinolide are 0.3 - 0.7 mg / L, 0.3 - 0.7 mg / L and 0.01 mg / L in sequence.

[0010] Specifically, the formula of the 1 / 2 Hoagland nutrient solution is:

[0011] Component Concentration (mg / L) <![CDATA[Ca(NO3)2·4H2O]]> 590 <![CDATA[KNO3]]> 253 <![CDATA[MgSO4·7H2O]]> 246 <![CDATA[NH4H2PO4]]> 58 <![CDATA[NaFeEDTA-3H2O]]> 10.5 <![CDATA[H3BO3]]> 1.43 <![CDATA[MnCl2·4H2O]]> 0.89 <![CDATA[ZnSO4·7H2O]]> 0.11 <![CDATA[CuSO4·5H2O]]> 0.04 <![CDATA[Na2MoO4·2H2O]]> 0.01 。

[0012] Specifically, the mass ratio of the α-naphthaleneacetic acid, gliotoxin and brassinolide is 50:50:1.

[0013] Specifically, the final concentrations of the α-naphthaleneacetic acid, gliotoxin and brassinolide are 0.5 mg / L, 0.5 mg / L and 0.01 mg / L in sequence.

[0014] The present invention also provides an application of the above-mentioned nutrient solution for water-cultured seedlings of Mesona chinensis in the cutting and seedling raising of Mesona chinensis.

[0015] The present invention also provides a method for water-culturing seedlings of Mesona chinensis, including the following steps:

[0016] S1. Select a healthy and disease-free mother plant, cut a 8 - 10 cm tender stem tip 0.5 cm below the stem node, leaving only 1 pair of top leaves and 1 - 2 lateral buds at the top to remove apical dominance, and use it as a cutting;

[0017] S2. Immerse the cutting in a 0.1% (w / v) thiophanate-methyl solution for disinfection for 10 min, take it out, and rinse it 3 times with sterile water, 1 min each time; immerse the lower end of the cutting in the nutrient solution for water-cultured seedlings of Mesona chinensis according to any one of claims 1 - 4 and culture for 25 - 30 d to obtain water-cultured seedlings of Mesona chinensis.

[0018] Specifically, the depth of the cutting immersed in the nutrient solution for water-cultured seedlings of Mesona chinensis is 3 - 5 cm.

[0019] Specifically, in S2, the culture conditions are as follows: 12 hours of light period at 25°C / 12 hours of dark period at 20°C, relative humidity of 70%, and light intensity of 220 μmol / (m 2 ·s).

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] The water culture nutrient solution for Mesona chinensis in the present invention is based on 1 / 2 Hoagland nutrient solution, and at the same time, α-naphthaleneacetic acid, guvermectin, and brassinolide components are added. When using this water culture nutrient solution to cultivate Mesona chinensis cuttings, the rooting rate, average number of roots, and incidence rate can reach 95%, 7.8 roots, and 19% respectively, indicating that there is a synergistic effect among the α-naphthaleneacetic acid, guvermectin, and brassinolide added in the nutrient solution of the present invention, which can improve the rooting and disease resistance effects of Mesona chinensis cuttings, and thus can provide support for the water culture seedling raising of Mesona chinensis. Specific Embodiments

[0022] The technical solutions of the present invention patent will be described clearly and completely below. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative efforts belong to the scope of protection of the present invention.

[0023] Guvermectin (CAS: 2004-04-8) is a nucleoside metabolite formed by Streptomyces hygroscopicus after fermentation. It is easily soluble in water and very stable at room temperature. As a plant growth regulator, guvermectin can effectively promote the growth and development of economic crops such as wheat, potato, and corn, increase the pest and disease resistance of crops, and has the effects of increasing production, stabilizing production, and improving quality.

[0024] 1. Materials and Methods

[0025] 1.1 Tested Mesona chinensis

[0026] Mesona chinensis of "Lingshan Big Leaf Variety"

[0027] 1.2 Tested 1 / 2 Hoagland nutrient solution

[0028] See Table 1.

[0029] Table 1 Formulation of 1 / 2 Hoagland nutrient solution

[0030] Component Concentration (mg / L) <![CDATA[Ca(NO3)2·4H2O]]> 590 <![CDATA[KNO3]]> 253 <![CDATA[MgSO4·7H2O]]> 246 <![CDATA[NH4H2PO4]]> 58 <![CDATA[NaFeEDTA·3H2O]]> 10.5 <![CDATA[H3BO3]]> 1.43 <![CDATA[MnCl2·4H2O]]> 0.89 <![CDATA[ZnSO4·7H2O]]> 0.11 <![CDATA[CuSO4·5H2O]]> 0.04 <![CDATA[Na2MoO4·2H2O]]> 0.01

[0031] 1.3 Tested nutrient solution

[0032] See Table 2.

[0033] Table 2 Tested nutrient solution

[0034]

[0035] 1.4 Test spore suspension

[0036] Fusarium oxysporum isolated from the roots of diseased Mesona chinensis plants was inoculated onto PDA medium. After culturing at 25 °C for 7 days, the spores were scraped off and formulated into a spore suspension of 1×10 6 cfu / mL with sterile water.

[0037] 1.5 Rooting test

[0038] Healthy and disease-free mother plants were selected, and 10-cm tender stem tips were cut from 0.5 cm below the stem nodes, leaving only 1 pair of top leaves and 1 lateral bud at the top to remove apical dominance as cuttings. The cuttings were soaked and disinfected in a 0.1% (w / v) thiophanate-methyl solution for 10 min, taken out, and rinsed 3 times with sterile water for 1 min each time. The lower ends of the cuttings were immersed in the test nutrient solutions in Table 2 for cultivation. Each test nutrient solution had 4 replicates, with 25 cuttings in each replicate, and the distance between adjacent 2 cuttings was 20 cm. The depth of the cuttings immersed in the Mesona chinensis hydroponic seedling nutrient solution was 4 cm; the culture conditions were: 12 h light period at 25 °C / 12 h dark period at 20 °C, relative humidity 70%, and light intensity 220 μmol / (m 2 ·s).

[0039] The nutrient solution was changed every 2 days. When culturing to the 14th day, the rooting rate and average number of roots of the cuttings were investigated, and the results are shown in Table 3. Among them, only the roots with a root length ≥1 cm were counted during the investigation.

[0040] 1.5 Disease resistance test

[0041] When culturing to the 14th day, the test spore suspension was added to the nutrient solution, and the final concentration of the spore suspension was 1×10 4 cfu / mL. The nutrient solution was changed every 2 days and the test spore suspension was re-inoculated. The incidence rate was investigated on the 7th day after the first inoculation (when Fusarium oxysporum infects the roots of Mesona chinensis, it can cause the roots to turn brown and rot), and the results are shown in Table 3.

[0042] Table 3 Effects of different nutrient solutions on the rooting of cuttings

[0043]

[0044] In Table 3, the rooting rate is the ratio of the number of rooted cuttings to the total number of cuttings; the average number of roots is the ratio of the total number of roots of the rooted cuttings to the total number of rooted cuttings; the incidence rate is the ratio of the number of diseased cuttings to the total number of cuttings.

[0045] As can be seen from Table 3, using α-naphthaleneacetic acid, glabridin or brassinolide alone can improve the rooting rate, the number of roots and the disease resistance of Mesona chinensis cuttings. When α-naphthaleneacetic acid, glabridin and brassinolide are used in combination of two or all three to treat Mesona chinensis cuttings, the rooting effect and disease resistance effect of the cuttings can be further improved. Especially when all three are used together, the rooting rate, the average number of roots and the incidence rate are 95%, 7.8 roots and 19% respectively, and the effects of promoting rooting and disease resistance are significantly better than those of other experimental groups. This shows that there is a synergistic effect among α-naphthaleneacetic acid, glabridin and brassinolide added in the nutrient solution of the present invention, which can improve the rooting and disease resistance effects of Mesona chinensis cuttings, and thus can provide support for the hydroponic seedling cultivation of Mesona chinensis.

[0046] The foregoing description of the specific exemplary embodiments of the present invention is for purposes of illustration and exemplification. These descriptions are not intended to limit the invention to the precise forms disclosed, and obviously, many modifications and variations are possible in light of the above teachings. The purpose of selecting and describing the exemplary embodiments is to explain the specific principles of the present invention and its practical applications, so that those skilled in the art can implement and utilize various different exemplary embodiments of the present invention, as well as various different selections and modifications. The scope of the present invention is intended to be defined by the claims and their equivalents.

Claims

1. A nutrient solution for cultivating seedlings of Mesona chinensis Benth. in water, characterized in that, It is based on 1 / 2 Hoagland nutrient solution, and naphthaleneacetic acid, glabridin and brassinolide are added; the final concentrations of the α-naphthaleneacetic acid, glabridin and brassinolide are 0.3-0.7 mg / L, 0.3-0.7 mg / L and 0.01 mg / L in sequence.

2. The Mesona chinensis Benth. water culture seedling nutrient solution according to claim 1, characterized in that The formula of the 1 / 2 Hoagland nutrient solution is as follows: 。 3. The nutrient solution for cultivating seedlings of Mesona chinensis Benth. in water according to claim 1, wherein, The mass ratio of the α-naphthaleneacetic acid, glabridin and brassinolide is 50:50:

1.

4. The Mesona chinensis water culture seedling nutrient solution according to claim 1, characterized in that, The final concentrations of the α-naphthaleneacetic acid, glabridin and brassinolide are 0.5 mg / L, 0.5 mg / L and 0.01 mg / L in sequence.

5. An application of the Mesona chinensis water culture seedling nutrient solution according to any one of claims 1_4 in the cuttage seedling raising of Mesona chinensis.

6. A method for hydroponic seedling of Mesona chinensis Benth., characterized in that, It includes the following steps: S1. Select healthy and disease-free mother plants, cut 8-10 cm tender stem tips 0.5 cm below the stem nodes, leaving only 1 pair of young leaves and 1-2 lateral buds at the top to remove apical dominance as cuttings. S2. After disinfection, immerse the lower ends of the cuttings in the Mesona chinensis water culture seedling nutrient solution according to any one of claims 1_4 and culture for 25-30 d to obtain Mesona chinensis water-cultured seedlings.

7. The method for cultivating seedlings of Mesona chinensis Benth. in water according to claim 1, characterized in that The depth of the cuttings immersed in the Mesona chinensis water culture seedling nutrient solution is 3-5 cm.

8. The method for cultivating seedlings of Mesona chinensis Benth. in water according to claim 1, wherein In S2, the culture conditions are as follows: photoperiod of 12 h at 25 °C / dark period of 12 h at 20 °C, relative humidity of 70%, and light intensity of 220 μmol / (m 2 ·s).

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