Microalgae fermentation liquor fertilizer as well as preparation method and application thereof

Abstract

The invention relates to the technical field of biological fertilizers, in particular to a microalgae fermentation liquor fertilizer and a preparation method and application thereof, and the fertilizer comprises the following components: microalgae fermentation liquor and medium trace elements. By designing the microalgae biological fertilizer, the microalgae biological fertilizer contains nutrient elements required in the crop growth and development process, crop growth and yield improvement are promoted while crop growth is guaranteed, the vitality of crops is remarkably enhanced, and the microalgae biological fertilizer becomes a part of a crop self-regulation mechanism. The complex physiological functions of the plants can be accurately regulated and controlled, and an excellent environment is provided for healthy growth and survival of the plants. In addition, the microalgae fertilizer is completely non-toxic, harmless and pollution-free, meets the environmental protection standard, and is a preferable fertilizer for producing organic, green and pollution-free agricultural products.

Description

Technical Field

[0001] The present invention relates to the technical field of biological fertilizers, and particularly to a microalgae fermentation broth fertilizer and its preparation method and application. Background Art

[0002] Microalgae are single-celled algae with small individuals, simple structures, and high photosynthetic utilization. They are widely distributed in different environments such as soil, oceans, rivers, and lakes, and even live in some extreme environments (the Arctic and Antarctic, arid and saline soils, etc.). Microalgae fermentation broth is the product of high-density fermentation of microalgae, which contains rich nutrients and has high development value. The advantages of microalgae fermentation broth as the core component of microalgae fermentation broth fertilizer are as follows: (1) Microalgae fermentation broth contains macroelements such as nitrogen, phosphorus, potassium, and sulfur, which can fully meet the growth needs of plants; (2) Microalgae fermentation broth contains various substances such as amino acids, soluble polysaccharides, retinol, lipids, chlorophyll, and vitamins, which can be used for plant growth regulation, promoting early flowering, fruit setting, and fruit ripening of fruit crops, increasing production, promoting leaf growth of leafy vegetables, and achieving the purpose of increasing production and weight; (3) The plant hormones in microalgae fermentation broth have physiological effects such as regulating plant stomatal conductance, chlorophyll accumulation, and root development, and improving the stress resistance of plants under stress conditions such as high temperature, drought, and high salt.

[0003] By applying advanced microalgae fermentation technology, we can produce a stable and reliable microalgae fermentation broth fertilizer. This microalgae fermentation broth fertilizer is not affected by external environmental changes and can completely avoid the risk of heavy metal pollution. It effectively solves the problems of food safety and environmental pollution caused by overuse of chemical fertilizers, providing the possibility for realizing green and healthy urban agriculture. Summary of the Invention

[0004] In response to the need to develop new biological fertilizers to replace traditional chemical fertilizers, the present invention provides a microalgae fermentation broth fertilizer and its preparation method and application, which can provide sufficient nutrients for crops while avoiding food safety problems and environmental pollution problems.

[0005] In a first aspect, the present invention provides a microalgae fermentation broth fertilizer, comprising the following components: microalgae fermentation broth, medium and trace elements, and a pH regulator.

[0006] Further, the fertilizer comprises the following components in parts by weight: 50-90 parts of microalgae fermentation broth, 10-20 parts of medium and trace elements, and 5-10 parts of pH regulator. Further, the medium and trace elements are one or more of boric acid, potassium dihydrogen phosphate, ferric sulfate, copper sulfate, potassium sulfate, and zinc sulfate.

[0007] Further, the pH regulator is a food additive.

[0008] Second aspect, the present invention provides a method for preparing a microalgae fermentation broth fertilizer, comprising the following steps:

[0009] (1) Fermentation broth extraction: After the fermentation of the microalgae fermentation supernatant is completed, it is allowed to settle overnight in a hydrolysis tank and then centrifuged. The supernatant is taken, and the centrifuged supernatant is poured into a batching container. After the foam is completely broken, it is used for subsequent steps;

[0010] (2) Auxiliary material preparation: Select medium and trace elements and add them to the batching container in turn while stirring and heating. Stir well until all components are dissolved. After cooling to room temperature, add a pH regulator to adjust the pH to obtain the finished product of the microalgae fermentation broth fertilizer.

[0011] Further, in step (1), the microalgae is Euglena Gracilis CGMCC No. 40684.

[0012] Further, in step (1), the batching container is an integrated device for stirring and heating.

[0013] Further, in step (2), the process conditions for the preparation of auxiliary materials are: stirring speed 300 - 600 rpm, temperature 25 - 50 °C.

[0014] Third aspect, the present invention provides an application of the microalgae fermentation broth fertilizer in crop planting.

[0015] The beneficial effects of the present invention are as follows:

[0016] (1) When the present invention is applied to newly planted crops, it can strengthen the growth of crop roots, make the root and stem thicker, and promote the absorption of various nutrients required for crop growth.

[0017] (2) The present invention can effectively promote the rapid seedling growth of crops, significantly accelerate their growth process, and ultimately achieve an increase in crop yield and income, bringing tangible economic benefits to agricultural production.

[0018] (3) The present invention uses a safe, reliable, green and pollution-free microalgae fermentation broth as the core raw material, without adding any toxic and harmful substances. The prepared microalgae fermentation broth fertilizer can effectively meet the development needs of green agriculture and provide strong support for the sustainable development of green agriculture. Description of the Drawings

[0019] Figure 1 It is a comparison chart of the average weight per plant of the fertilizer group and the control group. Detailed Embodiments

[0020] In order to help experts in the field better understand the technical details of the present invention, the technical solutions in the embodiments of the present invention are clearly and completely described below. It should be clear that these described embodiments do not represent all possible implementation methods of the present invention. Based on these embodiments, any other implementation methods that can be thought of by any person skilled in the art without performing innovative work should be regarded as the protection scope of the present invention.

[0021] The microalgae strain used in this example is the mutant strain ZWang109 of Euglena (Euglenagracilis), which was deposited in the General Microbiology Center of China Microorganism Culture Collection Administration on June 28, 2023. The deposit address is: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number is: CGMCC No.40684.

[0022] The pH regulator used in the examples is food grade 98.5% flaky sodium hydroxide produced by Shandong Binhua Group Co., Ltd.

[0023] Embodiment 1 The method for preparing microalgae fermentation liquid fertilizer comprises the following steps:

[0024] (1) After 9 days of fermentation, the microalgae fermentation liquid was placed in a hydrolysis tank overnight for sedimentation, crushing, and then centrifuged. The supernatant was poured into a transfer container and the foam was completely crushed before subsequent use.

[0025] (2) 50 parts of microalgae fermentation liquid and 5 parts of pH adjuster are poured into a stirring tank with a heating function, the stirring speed is 300-600 rpm, the pH is adjusted to 6-7, and then cooled to room temperature;

[0026] (3) 10 parts of medium and trace elements are selected and added to the microalgae fermentation liquid after the acid-base adjustment, wherein the medium and trace elements are boric acid, potassium dihydrogen phosphate, iron sulfate, copper sulfate, potassium sulfate, and zinc sulfate. The stirring speed is 300-600 rpm, and the stirring temperature is 25-50° C. After being fully mixed, the microalgae fermentation liquid fertilizer can be obtained.

[0027] Embodiment 2 The method for preparing microalgae fermentation liquid fertilizer comprises the following steps:

[0028] (1) After 9 days of fermentation, the microalgae fermentation liquid was placed in a hydrolysis tank overnight for sedimentation, crushing, and then centrifuged. The supernatant was poured into a transfer container and the foam was completely crushed before subsequent use.

[0029] (2) 60 parts of microalgae fermentation liquid and 7 parts of pH adjuster are poured into a stirring tank with a heating function, the stirring speed is 300-600 rpm, the pH is adjusted to 6-7, and then cooled to room temperature;

[0030] (3) Select 15 kinds of medium and trace elements and add them to the microalgae fermentation broth whose acid-base has been adjusted. The medium and trace elements are boric acid, potassium dihydrogen phosphate, ferric sulfate, copper sulfate, potassium sulfate, and zinc sulfate. The stirring speed is 300 - 600 rpm, and the stirring temperature is 25 - 50 °C. After thorough mixing, the microalgae fermentation broth fertilizer can be obtained.

[0031] Example 3 Preparation method of microalgae fermentation broth fertilizer, including the following steps:

[0032] (1) Let the microalgae fermentation broth after 9 days of fermentation settle overnight in a hydrolysis tank, break it, and then centrifuge it. Take the supernatant and pour it into a transfer container. After waiting for the foam to completely break, proceed with subsequent use;

[0033] (2) Pour 80 parts of microalgae fermentation broth and 10 parts of pH regulator into a stirring tank with a heating function in sequence. The stirring speed is 300 - 600 rpm. After adjusting the pH to 6 - 7, cool it to room temperature;

[0034] (3) Select 20 kinds of medium and trace elements and add them to the microalgae fermentation broth whose acid-base has been adjusted. The medium and trace elements are boric acid, potassium dihydrogen phosphate, ferric sulfate, copper sulfate, potassium sulfate, and zinc sulfate. The stirring speed is 300 - 600 rpm, and the stirring temperature is 25 - 50 °C. After thorough mixing, the microalgae fermentation broth fertilizer can be obtained. Set a comparative example. The difference between the comparative example and Example 1 is that the comparative example uses clear water.

[0035] Taking lettuce crops as an example, dilute the fertilizer obtained in the example and apply it at 100 kg / mu. Apply the same weight of clear water in the comparative example. Fertilize twice after planting and harvest after about 30 days. The single-plant mass of lettuce after harvest in different examples and the comparative example is shown in Table 1.

[0036] Table 1 Single-plant mass data of lettuce after harvest in different examples and the comparative example

[0037] Item Quality of single lettuce plant (g) Example 1 58.19 Example 2 55.26 Example 3 54.31 Comparative example 49.16

[0038] As can be seen from Table 1, the microalgae fermentation broth fertilizer of the present invention can effectively increase the yield of crops and promote the growth and yield increase of crops.

[0039] Although the present invention has been described in detail by way of preferred embodiments, the present invention is not limited thereto. Without departing from the spirit and essence of the present invention, those of ordinary skill in the art can make various equivalent modifications and substitutions to the embodiments of the present invention, and these modifications and substitutions should all be within the scope covered by the present invention / Any person familiar with the technical field of the present invention can easily think of changes and substitutions within the technical scope disclosed by the present invention, and all should be covered within the protection scope of the present invention.

Claims

1. A microalgae fermentation broth fertilizer, characterized in that, It includes the following components: microalgae fermentation broth, medium and trace elements, and pH regulator.

2. The microalgae fermentation broth fertilizer according to claim 1, characterized in that, The microalgae fermentation broth is an extraction broth of a certain alga in the Chlorophyta phylum.

3. The microalgae fermentation broth fertilizer according to claim 1, wherein It includes the following components in parts by weight: 50 - 90 parts of microalgae fermentation broth, 10 - 20 parts of medium and trace elements, and 5 - 10 parts of pH regulator.

4. The microalgae fermentation broth fertilizer according to claim 1, wherein The medium and trace elements are one or more of boric acid, potassium dihydrogen phosphate, ferric sulfate, copper sulfate, potassium sulfate, and zinc sulfate.

5. The microalgae fermentation broth fertilizer according to claim 1, characterized in that, The pH regulator is a food additive.

6. The manufacturing method of a microalgae fermentation broth fertilizer formula according to claim 1, characterized in that, The specific steps are as follows: S1, Fermentation broth extraction: Pour the supernatant of the fermented microalgae fermentation broth into a batching container after sedimentation and centrifugation, and wait for subsequent use; S2, Acid-base adjustment: Select a pH regulator and add it to the microalgae fermentation broth and stir to adjust it to neutral; S3, Auxiliary material preparation: Select medium and trace elements and add them to the microalgae fermentation broth that has completed acid-base adjustment, and heat while stirring to obtain the finished product of the microalgae fermentation broth fertilizer.

7. A microalgae fermentation broth fertilizer formula and its preparation method according to claim 4, characterized in that, The batching container in S1 is a stirring tank of a device that combines stirring and heating.

8. A microalgae fermentation broth fertilizer formula and its preparation method according to claim 4, characterized in that, The stirring speed in S3 is 300 - 600 rpm, and the temperature is 25 - 50 °C.

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