Fertilizer synergist A and application thereof

Abstract

The invention belongs to the technical field of fertilizer synergists, and particularly relates to a fertilizer synergist A and application thereof, and the fertilizer synergist A comprises the following components by weight: 8-12 parts of mannan oligosaccharide; 13-17 parts by weight of biological fermentation compound amino acid; 0.1 to 5 parts by weight of a plant growth regulator; 1-15 parts by weight of a soil structure improver; and 1-8 parts by weight of composite trace elements. The fertilizer synergist provided by the invention can effectively improve the absorption and utilization of crops, especially wheat, on the fertilizer, and has positive effects on improving the yield and stress resistance of the crops.

Description

Technical Field

[0001] The present invention belongs to the technical field of fertilizer synergists, and particularly relates to a fertilizer synergist A and its application. Background Art

[0002] Fertilizers can provide essential nutrients for plants, improve soil properties, and enhance soil fertility, and are one of the material bases for agricultural production. However, the utilization rate of fertilizers is relatively low. During the application stage, fertilizers will be lost through volatilization, leaching, and runoff. This will not only lead to insufficient nutrient supply for plants but also damage farmland, resulting in problems such as eutrophication of surface water, excessive nitrate in crops, and serious pollution of lakes.

[0003] Fertilizer synergists are a class of active substances aimed at increasing nutrient availability. They mainly increase the nutrient supply to crops by fixing nitrogen and activating phosphorus and potassium elements that are difficult to be utilized in the soil, and assist plants in regulating their physiological and biochemical activities to promote plant growth and enhance their stress resistance. Usually, they are added to conventional fertilizers as auxiliaries in order to appropriately reduce the amount of fertilizers used and improve the fertilizer utilization rate.

[0004] However, there are certain problems with common synergists on the market at present in terms of application scope, pollution degree, and utilization rate. For example, urease inhibitors and nitrification inhibitors will dilute their concentrations when encountering a large amount of water, reducing their synergistic efficiency; the coating technology is difficult to be degraded after playing the slow-release role and is likely to pollute the soil environment; the polyglutamic acid synergist is difficult to play a long-term role due to too fast degradation, resulting in a significant reduction in the synergistic efficiency.

[0005] Therefore, developing a new fertilizer synergist to expand its application area and reduce environmental pollution while achieving long-term slow release is of extremely important significance for the current development of the fertilizer industry and ecological protection. Summary of the Invention

[0006] In view of the above-mentioned prior art, the present invention provides a fertilizer synergist, its preparation method and application to solve the technical problems of high pollution, low efficiency, and small application scope faced by existing fertilizer synergists.

[0007] To achieve the above object, the technical solution adopted by the present invention is to provide a fertilizer synergist, which comprises the following components in parts by mass:

[0008] 10 parts by weight of mannan oligosaccharide;

[0009] 15 parts by weight of bio-fermented compound amino acids;

[0010] 0.1 part by weight - 5 parts by weight of plant growth regulators;

[0011] 1 part by weight - 15 parts by weight of soil structure improvers;

[0012] 1 to 8 parts by weight of compound trace elements.

[0013] The fertilizer synergist described in the present invention is composed of mannan oligosaccharide, bio-fermented compound amino acid, plant growth regulator, soil structure improver and compound trace elements.

[0014] The fertilizer synergist described in the present invention takes mannan oligosaccharide and bio-fermented compound amino acid as the main components. Among them, mannan oligosaccharide, as a biostimulant, can promote the growth of plant roots, enhance the nutrient absorption capacity of plants, and at the same time has a positive impact on the soil microbial community, contributing to soil health. Bio-fermented compound amino acid is a complex containing various amino acids, which mainly includes glutamic acid, aspartic acid, valine and leucine. They can be directly absorbed and utilized by plants to promote plant growth. At the same time, amino acids are also the food source of soil microorganisms, helping to maintain the diversity and activity of soil microorganisms.

[0015] In addition, the plant growth regulator in the fertilizer synergist described in the present invention can regulate the growth and development process of plants, improve the resistance of plants to adversity, and increase crop yield and quality. The plant growth regulator is mainly 5-ALA. The soil structure improver is one or more of chitosan, humic acid, fulvic acid, polyacrylamide (PAM), and polyvinyl alcohol (PVA). It can improve the soil structure, increase the water retention and air permeability of the soil, reduce nutrient loss, and at the same time has a certain adsorption and fixation effect on heavy metals and harmful substances in the soil, reducing environmental pollution. The compound trace elements are one or more of zinc sulfate, boric acid (borax), ammonium molybdate, copper sulfate, and manganese sulfate. These elements are crucial for plant growth and development, but are often lacking in the soil or not easily absorbed by plants. By adding compound trace elements, the utilization rate of these elements by plants can be improved.

[0016] Through the synergistic effect of the above components, the fertilizer synergist of the present invention can not only improve the nutrient utilization rate of fertilizers and reduce nutrient loss, but also improve the soil environment and reduce environmental pollution.

[0017] The present invention also discloses a preparation method of the marketed fertilizer synergist, which includes the following steps: taking the formulated amounts of mannan oligosaccharide, bio-fermented compound amino acid, plant growth regulator, soil structure improver and compound trace elements, crushing them to more than 40 meshes, mixing them evenly in proportion, and then inspecting, weighing and packaging.

[0018] Compared with the prior art, the beneficial effects of the present invention are:

[0019] 1. The composition of the fertilizer synergist in the present invention is relatively simple. The components interact with each other, can provide suitable growth conditions for crops, and can enhance the physiological activity of crops, thereby improving the utilization efficiency of fertilizers by crops.

[0020] 2. The fertilizer synergist of the present invention is rich in mannan oligosaccharide. As a biostimulant, it can not only promote the growth of plant roots, enhance the nutrient absorption capacity of plants, but also have a positive impact on the soil microbial community, contribute to soil health, and effectively improve the utilization rate of fertilizers.

[0021] 3. The fertilizer synergist of the present invention contains bio-fermented compound amino acids, which can not only adsorb ammoniacal nitrogen to reduce volatilization loss, stimulate the growth of plant roots and the absorption and utilization of nutrients, but also supplement the amino acids required by plants to promote plant growth.

[0022] 4. The fertilizer synergist of the present invention can effectively improve the absorption and utilization of fertilizers by crops, especially wheat, and has a positive effect on increasing crop yields. Description of the Drawings

[0023] Figure 1 Indices of chlorophyll a, b and total chlorophyll content

[0024] Figure 2 Indices of proline and MDA content

[0025] Figure 3 Indices of catalase, superoxide dismutase and peroxidase activities Detailed Embodiments

[0026] The embodiments of the present invention disclose a fertilizer synergist and a preparation method thereof.

[0027] To further understand the present invention, the present invention will be described in detail below in conjunction with embodiments.

[0028] Example 1: Fertilizer Synergist and Its Preparation Method

[0029] 10 parts by weight of mannan oligosaccharide, 15 parts by weight of bio-fermented compound amino acids (mass ratio of glutamic acid, aspartic acid, valine and leucine is 1:1:1:4), 2 parts by weight of 5-ALA, 8 parts by weight of chitosan, 10 parts by weight of humic acid, 5 parts by weight of zinc sulfate, 2 parts by weight of boric acid (borax), 1 part by weight of copper sulfate.

[0030] The preparation method is to separately pulverize mannan oligosaccharide, bio-fermented compound amino acids, 5-ALA, humic acid, zinc sulfate, boric acid (borax) and copper sulfate to more than 40 mesh, mix them evenly in proportion, and then inspect, weigh and package.

[0031] Example 2: Fertilizer Synergist and Its Preparation Method

[0032] 10 parts by weight of mannan oligosaccharide, 15 parts by weight of bio-fermented compound amino acids (the mass ratio of glutamic acid, aspartic acid, valine and leucine is 1:1:1:4), 2 parts by weight of 5-ALA, 8 parts by weight of chitosan, 4 parts by weight of fulvic acid, 5 parts by weight of ammonium molybdate, 2 parts by weight of boric acid (borax), 1 part by weight of manganese sulfate.

[0033] The preparation method is to crush mannan oligosaccharide, bio-fermented compound amino acids, 5-ALA 2, chitosan, fulvic acid, ammonium molybdate, boric acid (borax) and manganese sulfate to more than 40 meshes respectively, mix them evenly in proportion, and then inspect, weigh and package.

[0034] Example 3: Effect of mannan oligosaccharide addition amount on the growth of wheat seedlings

[0035] Through the pot experiment of wheat seedlings, verify the most suitable addition amount of mannan oligosaccharide for the growth of wheat seedlings in the fertilizer synergist.

[0036] 1. Materials and methods

[0037] 1.1 Test materials:

[0038] C: 6 parts by weight of mannan oligosaccharide, 15 parts by weight of bio-fermented compound amino acids (the mass ratio of glutamic acid, aspartic acid, valine and leucine is 1:1:1:4), 2 parts by weight of 5-ALA, 8 parts by weight of chitosan, 10 parts by weight of humic acid, 5 parts by weight of zinc sulfate, 2 parts by weight of boric acid (borax), 1 part by weight of copper sulfate.

[0039] B: 8 parts by weight of mannan oligosaccharide, 15 parts by weight of bio-fermented compound amino acids (the mass ratio of glutamic acid, aspartic acid, valine and leucine is 1:1:1:4), 2 parts by weight of 5-ALA, 8 parts by weight of chitosan, 10 parts by weight of humic acid, 5 parts by weight of zinc sulfate, 2 parts by weight of boric acid (borax), 1 part by weight of copper sulfate.

[0040] A: 10 parts by weight of mannan oligosaccharide, 15 parts by weight of bio-fermented compound amino acids (the mass ratio of glutamic acid, aspartic acid, valine and leucine is 1:1:1:4), 2 parts by weight of 5-ALA, 8 parts by weight of chitosan, 10 parts by weight of humic acid, 5 parts by weight of zinc sulfate, 2 parts by weight of boric acid (borax), 1 part by weight of copper sulfate.

[0041] D: 12 parts by weight of mannan oligosaccharide, 15 parts by weight of bio-fermented compound amino acids (the mass ratio of glutamic acid, aspartic acid, valine and leucine is 1:1:1:4), 2 parts by weight of 5-ALA, 8 parts by weight of chitosan, 10 parts by weight of humic acid, 5 parts by weight of zinc sulfate, 2 parts by weight of boric acid (borax), 1 part by weight of copper sulfate.

[0042] E: 14 parts by weight of mannan oligosaccharide, 15 parts by weight of bio-fermented compound amino acids (the mass ratio of glutamic acid, aspartic acid, valine and leucine is 1:1:1:4), 2 parts by weight of 5-ALA, 8 parts by weight of chitosan, 10 parts by weight of humic acid, 5 parts by weight of zinc sulfate, 2 parts by weight of boric acid (borax), 1 part by weight of copper sulfate.

[0043] Take the obtained fertilizer synergist C, B, A, D, E and add them to the ordinary fertilizer according to a weight ratio of 1% to obtain C, B, A, D, E synergistic fertilizers.

[0044] The wheat seeds are Ruihua Wheat 596

[0045] 1.2 Experimental design

[0046] A total of five groups of treatments were set up in the experiment, with three replicates designed for each group, and each pot was a replicate with ten seedlings in each pot.

[0047] Treatment C: Apply synergistic fertilizer C with a fertilizer concentration of 1 g / Kg;

[0048] Treatment B: Apply synergistic fertilizer B with a fertilizer concentration of 1 g / Kg;

[0049] Treatment A: Apply synergistic fertilizer A with a fertilizer concentration of 1 g / Kg;

[0050] Treatment D: Apply synergistic fertilizer D with a fertilizer concentration of 1 g / Kg

[0051] Treatment E: Apply synergistic fertilizer E with a fertilizer concentration of 1 g / Kg

[0052] 1.3 Experimental methods.

[0053] (1) Soak and germinate the wheat seeds two days in advance for the experiment, and prepare the fertilizers with corresponding concentrations.

[0054] (2) Use the fertilizers with corresponding concentrations as the base fertilizers respectively, add an equal amount of water, and top-dress the fertilizers with corresponding concentrations after seven days.

[0055] (3) After culturing for a period of time, sample to measure the fresh weight, dry weight and plant height of wheat seedlings.

[0056] 2 Experimental results

[0057] It can be seen from Table 1 that with the increase of the addition amount of mannan oligosaccharide, the fresh weight, dry weight and plant height of wheat seedlings all show a trend of first increasing and then decreasing. Among them, the fresh weight, dry weight and plant height of wheat seedlings in treatment group A are significantly higher than those in other treatment groups. This indicates that when the addition amount of mannan oligosaccharide is 10 parts by weight, the growth promotion effect on wheat seedlings is the best.

[0058] Treatment group Fresh weight (g) Dry weight (g) Plant height (cm) C 1.76±0.08e 0.35±0.04d 23.35±1.16e B 2.40±0.06c 0.54±0.04c 28.58±0.43c A 4.00±0.14a 0.95±0.07a 36.74±1.48a D 2.67±0.11b 0.67±0.05b 31.56±1.09b E 2.14±0.08d 0.45±0.04c 26.12±0.91d

[0059] Example 4: Synergistic Fertilizer Containing Fertilizer Synergist

[0060] Take the fertilizer synergist prepared in Example 1 and add it to the ordinary fertilizer at a weight ratio of 1% to obtain the synergistic fertilizer.

[0061] Control Example 1:

[0062] Take the fertilizer synergist with the following formula and add it to the ordinary fertilizer at a weight ratio of 1% to obtain the synergistic fertilizer.

[0063] 10 parts by weight of mannan oligosaccharide, 15 parts by weight of bio-fermented composite f amino acids (the mass ratio of glutamic acid, aspartic acid, valine and leucine is 1:1:1:4), 2 parts by weight of 5-ALA, 18 parts by weight of humic acid, 5 parts by weight of zinc sulfate, 2 parts by weight of boric acid (borax), 1 part by weight of copper sulfate.

[0064] Control Example 2:

[0065] Take the fertilizer synergist with the following formula and add it to the ordinary fertilizer at a weight ratio of 1% to obtain the synergistic fertilizer.

[0066] 10 parts by weight of mannan oligosaccharide, 15 parts by weight of bio-fermented composite amino acids (the mass ratio of glutamic acid, aspartic acid, valine and leucine is 1:1:1:4), 2 parts by weight of compound nitrophenolate sodium, 8 parts by weight of chitosan, 10 parts by weight of humic acid, 5 parts by weight of zinc sulfate, 2 parts by weight of boric acid (borax), 1 part by weight of copper sulfate.

[0067] Example 5: Effects of Synergistic Fertilizer on the Growth and Salt-Alkali Tolerance of Wheat Seedlings

[0068] Through the pot experiment of wheat seedlings, comprehensively investigate the synergistic effect of the synergistic fertilizer added with the fertilizer synergist of the present invention, and verify its effects on the growth and salt-alkali tolerance of wheat seedlings.

[0069] 2. Materials and Methods

[0070] 1.1 Experimental materials: Synergistic fertilizers prepared in Example 4, Control Example 1, and Control Example 2; Conventional fertilizers purchased from the fertilizer market; Wheat seeds are Ruihua Wheat 596

[0071] 1.2 Experimental design

[0072] A total of seven groups of treatments were set in the experiment, with three replicates designed for each group, and each pot was taken as a replicate, with ten seedlings in each pot.

[0073] Treatment 1: Blank control (CK), applying conventional fertilizer;

[0074] Treatment 2: Applying the synergistic fertilizer prepared in Control Example 1, with a fertilizer concentration of 1 g / Kg

[0075] Treatment 3: Apply the synergistic fertilizer prepared in Comparative Example 2 with a fertilizer concentration of 1 g / Kg

[0076] Treatment 4: Apply the synergistic fertilizer prepared in Example 4 with a fertilizer concentration of 1 g / Kg

[0077] Treatment 5: Apply the synergistic fertilizer prepared in Comparative Example 1 with a fertilizer concentration of 1 g / Kg; add saline-alkali treatment with a saline-alkali concentration of 50 mmol / L

[0078] Treatment 6: Apply the synergistic fertilizer prepared in Comparative Example 2 with a fertilizer concentration of 1 g / Kg; add saline-alkali treatment with a saline-alkali concentration of 50 mmol / L

[0079] Treatment 7: Apply the synergistic fertilizer prepared in Example 4 with a fertilizer concentration of 1 g / Kg; add saline-alkali treatment with a saline-alkali concentration of 50 mmol / L

[0080] 1.3 Test method

[0081] (1) Soak and germinate wheat seeds two days in advance for experimental use, and prepare fertilizers with corresponding concentrations

[0082] (2) Use fertilizers with corresponding concentrations as base fertilizers respectively, add equal amounts of water, and top-dress fertilizers with corresponding concentrations after seven days

[0083] (3) After culturing for a period of time, take samples to measure the contents of chlorophyll, proline and malondialdehyde (MDA) in wheat seedlings, as well as the activities of catalase, superoxide dismutase and peroxidase

[0084] 2. Test results

[0085] As Figure 1 shown, under normal conditions, compared with the control group of Treatment 1, the contents of chlorophyll a, b and total chlorophyll in wheat seedlings increased significantly after fertilization. Among them, the chlorophyll content of wheat seedlings treated with the synergistic fertilizer prepared in Example 4 increased most significantly. It shows that the synergistic fertilizer prepared in Example 4 can enhance the photosynthesis of wheat seedlings and promote the growth of wheat seedlings. Under saline-alkali stress, the chlorophyll content of wheat seedlings decreased significantly. Compared with Treatments 5 and 6, the contents of chlorophyll a, b and total chlorophyll in wheat seedlings treated with the synergistic fertilizer prepared in Example 4 increased significantly. It shows that the synergistic fertilizer prepared in Example 4 can effectively inhibit saline-alkali stress and improve the salt tolerance of wheat seedlings

[0086] Proline is one of the important osmotic adjustment substances in plants, which can help plants maintain osmotic balance. Malondialdehyde (MDA) is one of the important indicators to measure the degree of oxidative stress in plants, which can reflect the degree of membrane lipid peroxidation in plants. As Figure 2As shown, under saline-alkali stress, the contents of proline and MDA in wheat seedlings increased significantly, indicating that under saline-alkali stress, wheat seedlings suffered from osmotic imbalance and oxidative stress. The contents of proline and malondialdehyde in wheat seedlings treated with the synergistic fertilizer prepared in Example 4 decreased significantly, and their contents were even lower than those in the control group of Treatment 1. This indicates that the synergistic fertilizer prepared in Example 4 can greatly promote plants to regulate osmotic stress and improve the ability to resist oxidative stress.

[0087] The activities of catalase, superoxide dismutase and peroxidase can reflect the degree of oxidative stress in plants. As Figure 3 shown, under normal conditions, the activities of catalase, superoxide dismutase and peroxidase in wheat seedlings treated with the synergistic fertilizer prepared in Example 4 decreased significantly. Under saline-alkali stress, the activities of catalase, superoxide dismutase and peroxidase in wheat seedlings increased significantly. After applying the common synergistic fertilizer and the synergistic fertilizer prepared in Example 4, the activities of catalase, superoxide dismutase and peroxidase in wheat seedlings decreased significantly, especially in the wheat seedlings of Treatment Group 7 treated with the synergistic fertilizer prepared in Example 4. This indicates that the synergistic fertilizer prepared in Example 4 has extremely significant effects in enhancing the salt tolerance and alkali resistance of wheat and resisting its oxidative stress.

[0088] In summary, the fertilizer synergist provided by the present invention, when mixed with ordinary fertilizers to form a synergistic fertilizer, can promote the physiological cycle and photosynthesis of plants after application, thereby efficiently supplementing nutrient elements for crops and promoting their growth. In addition, the fertilizer synergist shown in the present invention has extremely significant effects in improving the salt tolerance and alkali resistance of crops. It can regulate the proline content of crops and maintain the osmotic balance of crops. At the same time, it regulates the MDA content, improves the degree of membrane lipid peroxidation, and regulates the activities of catalase, superoxide dismutase and peroxidase to enhance the antioxidant capacity of crops. It can be applied to the preparation of more efficient synergistic fertilizers to promote the efficient growth of crops while improving their salt tolerance and alkali resistance.

[0089] The above-described embodiments are only used to describe the preferred embodiments of the present invention and do not limit the scope of the present invention. Without departing from the design spirit of the present invention, various deformations and improvements made by those of ordinary skill in the art to the technical solutions of the present invention shall fall within the protection scope determined by the claims of the present invention.

Claims

1. A fertilizer synergist A, characterized in that, It contains the following components in weight percentage: 8 - 12 parts by weight of mannan oligosaccharide; 13 - 17 parts by weight of bio - fermented compound amino acid; 0.1 part by weight - 5 parts by weight of plant growth regulator; 1 part by weight - 15 parts by weight of soil structure improver; 1 part by weight - 8 parts by weight of compound trace elements; The bio - fermented compound amino acid contains one or more of glutamic acid, aspartic acid, valine and leucine; The plant growth regulator contains 5 - ALA; The soil structure improver contains one or more of chitosan, humic acid, fulvic acid, polyacrylamide (PAM), polyvinyl alcohol (PVA); The compound trace elements contain one or more of zinc sulfate, boric acid (borax), ammonium molybdate, copper sulfate, manganese sulfate.

2. The fertilizer synergist A according to claim 1, characterized in that The bio - fermented compound amino acid contains glutamic acid, aspartic acid, valine and leucine, and the plant growth regulator contains 2 parts by weight of 5 - ALA; The soil structure improver contains chitosan and humic acid; The compound trace elements contain zinc sulfate, boric acid (borax) and copper sulfate.

3. The fertilizer synergist A according to claim 2, characterized in that, It contains the following components in weight percentage: 9 - 11 parts by weight of mannan oligosaccharide, 14 - 16 parts by weight of bio - fermented compound amino acid, 1 - 3 parts by weight of 5 - ALA, 7 - 9 parts by weight of chitosan, 9 - 11 parts by weight of humic acid, 4 - 6 parts by weight of zinc sulfate, 1 - 3 parts by weight of boric acid (borax), 0.5 - 2 parts by weight of copper sulfate. The bio - fermented compound amino acid is composed of glutamic acid, aspartic acid, valine and leucine, and the mass ratio of glutamic acid, aspartic acid, valine and leucine is 1 - 2:1 - 2:1 - 2:4 - 6.

4. The fertilizer synergist A according to claim 3, wherein It contains the following components in weight percentage: 10 parts by weight of mannan oligosaccharide, 15 parts by weight of bio - fermented compound amino acid, 2 parts by weight of 5 - ALA, 8 parts by weight of chitosan, 10 parts by weight of humic acid, 5 parts by weight of zinc sulfate, 2 parts by weight of boric acid (borax), 1 part by weight of copper sulfate; The bio - fermented compound amino acid is composed of glutamic acid, aspartic acid, valine and leucine, and the mass ratio of glutamic acid, aspartic acid, valine and leucine is 1:1:1:

4.

5. Use of the fertilizer synergist A according to any one of claims 1 - 4 for promoting the growth of wheat.

6. Use of the fertilizer synergist A according to any one of claims 1 - 4 for improving the resistance of wheat to saline - alkali stress.

Images