Polymerase amplification device and system for detecting African swine fever virus

Abstract

The invention belongs to the technical field of African swine fever virus detection, and particularly relates to a polymerase amplification device and system for African swine fever virus detection, and the device comprises a shell, and a first fixed plate, an amplification temperature control guide block heating part, a cold storage part, a heat dissipation part and a driving part which are integrally formed with the shell, and the amplification temperature control guide block is fixedly embedded in the first fixing plate. Compared with the prior art, the air outlet is formed in the bottom of the machine shell, the second fixing plate is arranged around the air outlet, the heat dissipation component slides in the second fixing plate in a limited mode, and the heat dissipation component can timely discharge accumulated heat generated by the cold storage component fixed to the upper portion of the heat dissipation component in the working process; the heating component is arranged between the amplification temperature control guide block and the cold storage component in a limited sliding mode, a heat preservation box is arranged in the machine shell, the heating component can be driven by the driving component to slide into the heat preservation box for heat insulation and heat preservation after completing heating of the amplification temperature control guide block, and waste heat in the heating component is stored and reused.

Description

Technical Field

[0001] The present invention belongs to the technical field of African swine fever virus detection, and particularly relates to a polymerase amplification device and system for African swine fever virus detection. Background Art

[0002] A polymerase chain reaction (hereinafter referred to as PCR for short, with the full English name of Polymerase chain reaction) amplification device is a device for amplifying specific DNA. Due to its strong specificity, high sensitivity, and rapidity, it is widely used in medical and biological laboratories, and can be used to determine whether a genetic disease map, an infectious disease diagnosis, gene replication, and paternity testing will be manifested in a specimen.

[0003] African swine fever virus is a large double-stranded DNA virus. PCR is a commonly used detection method. It is necessary to extract the viral DNA in pig blood and tissue samples, and achieve amplification through steps such as denaturation, annealing, and extension. Existing PCR amplification devices can automatically complete cyclic amplification. For example, a polymerase amplification device and system for African swine fever virus detection described in CN119144434A. The device includes a device casing and a fixed eaves integrally fixed with the device casing. An amplification temperature control guide block is fixedly arranged in the fixed eaves. A first heat conduction plate and a second pre-cooling plate are arranged below the amplification temperature control guide block. The first heat conduction plate and the second pre-cooling plate are on the same horizontal plane. This device can start refrigerating and storing cold during the denaturation heating process. When entering the annealing and cooling stage, it controls the second pre-cooling plate to be in thermal contact with the amplification temperature control guide block, so as to quickly cool the amplification temperature control guide block by using the stored cold.

[0004] However, the heat dissipation device in this device does not have a well-designed air duct, and the temperature control electric heating plate in the device is also directly exposed inside the box body. After multiple cycles, the heat accumulated inside the box cannot be discharged, which easily causes damage to the internal components of the device. Summary of the Invention

[0005] The present invention aims to provide a polymerase amplification device for African swine fever virus detection, mainly to solve the problem that the heat dissipation device in the existing polymerase amplification device does not have a well-designed air duct, and the temperature control electric heating plate in the device is also directly exposed inside the box body. After multiple cycles, the heat accumulated inside the box cannot be discharged, which easily causes damage to the internal components of the device.

[0006] To solve the above technical problems, the present invention provides the following technical solutions:

[0007] A polymerase amplification device for detecting African swine fever virus, comprising a casing, a first fixing plate integrally formed with the casing, and an amplification temperature control guide block. The amplification temperature control guide block is fixedly embedded in the first fixing plate. The device further includes a heating component, a cold storage component, a heat dissipation component, and a driving component. The heating component is limited and slidably arranged below the amplification temperature control guide block. An air outlet is formed at the bottom of the casing, and a second fixing plate is fixedly arranged around the air outlet. The heat dissipation component is longitudinally limited and slidably arranged in the second fixing plate. The cold storage component is fixedly installed on the upper part of the heat dissipation component, and the heating component is horizontally limited and slidably arranged on its upper part. A heat preservation box is further arranged inside the casing. One side of the heat preservation box is open and flush with the heating component. The driving component is used to drive the heating component to slide into the heat preservation box, and at the same time, it can also drive the cold storage component to rise and abut against the amplification temperature control guide block;

[0008] There are two groups of driving components. The two groups of driving components are installed on the bottom plate of the casing and located on both sides of the second fixing plate. The driving component includes a motor, a screw rod, a nut, a gear, and a rack. Two groups of first limiting plates and second limiting plates are arranged on both sides of the cold storage component. The first limiting plate and the second limiting plate are located in the same vertical plane and a second sliding groove is also arranged between them. The motor is fixedly connected to the bottom plate of the casing. The screw rod is fixedly connected to the rotating shaft of the motor and passes through the first limiting plate and the second limiting plate. The nut is sleeved on the screw rod and located between the first limiting plate and the second limiting plate. A second sliding block is arranged on the side of the nut, and the second sliding block is longitudinally limited and slidably arranged in the second sliding groove. The rack is fixedly installed on both sides of the heating component, and the gear is fixedly connected to the top of the screw rod and meshes with the rack.

[0009] Preferably, the heating component includes a first temperature guiding plate, a temperature control electric heating plate, and a heat insulation plate. The first temperature guiding plate is fixedly connected to the upper side of the temperature control electric heating plate, and the heat insulation plate is fixedly connected to the lower side of the temperature control electric heating plate. The first temperature guiding plate is horizontally limited and slidably arranged below the amplification temperature control guide block. The cold storage component further includes a semiconductor refrigeration sheet, a cold storage box, a sealing plate, a cold guiding sheet, and a second temperature guiding plate. The refrigerating side of the semiconductor refrigeration sheet is closely attached to the bottom of the cold storage box, and the heating side is fixedly connected to the heat dissipation component. The cold storage box is filled with a cold storage liquid. The sealing plate is closely attached to the upper part of the cold storage box. There are multiple cold guiding sheets fixedly connected to the lower side of the sealing plate and in contact with the cold storage liquid. The second temperature guiding plate is fixedly connected to the upper side of the sealing plate. The heat insulation plate is limited and slidably arranged on the upper part of the second temperature guiding plate. The first limiting plate, the second limiting plate, and the second sliding groove are all arranged on both sides of the cold storage box. Temperature sensors for temperature monitoring are respectively arranged in the amplification temperature control guide block, the first temperature guiding plate, and the second temperature guiding plate.

[0010] Preferably, the heat dissipation component includes a fan, a heat dissipation fin plate, and a first sliding block. The fan is fixedly connected to the lower part of the heat dissipation fin plate. The cold storage component is fixedly connected to the upper part of the heat dissipation fin plate. The first sliding block is arranged at both ends of the heat dissipation fin plate. First sliding grooves are longitudinally arranged at both ends of the second fixing plate. The heat dissipation fin plate is longitudinally limited and slidably arranged in the first sliding grooves of the second fixing plate through the first sliding block.

[0011] Preferably, a first air inlet and a second air inlet are further provided on the casing. The first air inlet is provided on both sides of the casing, and the second air inlet is provided on both sides of the second fixing plate.

[0012] Preferably, the heat preservation box includes a box body. One side of the box body is open, and the open side abuts against the amplification temperature control block. Third sliding grooves are transversely provided on both sides of the box body. The tooth bar is limited to slide in the third sliding grooves. A baffle and a second spring are further provided at the bottom of the third sliding grooves. The baffle is fixedly installed at one end of the second spring, and the other end of the second spring is fixedly connected to the bottom of the third sliding grooves.

[0013] Preferably, openings are provided on both sides of the cold storage box, and elastic sealing films are hermetically provided at the openings.

[0014] Preferably, a cover component is further included. The cover component includes a cylinder and a heat preservation cover. A cover notch is provided at the upper part of the open side of the box body. The cylinder is fixedly installed at one end of the second fixing plate close to the box body. The heat preservation cover is fixedly connected to the lower part of the push rod of the cylinder. A third limiting plate protrudes from the upper side of the heat preservation cover.

[0015] Preferably, the materials of both the heat preservation cover and the box body of the heat preservation box are materials with low heat conduction coefficients.

[0016] Preferably, the lower part of the heat preservation cover is wedge-shaped.

[0017] A system for detecting African swine fever virus includes the polymerase amplification device for detecting African swine fever virus according to any one of the claims, a sample processing module, a PCR reaction system configuration and a detection and analysis module. The sample is first processed by the sample processing module to extract the African swine fever virus nucleic acid therein, and then the PCR reaction system is configured. The extracted African swine fever virus nucleic acid, primers, deoxynucleoside triphosphates, buffer solution, heat-resistant DNA polymerase and an appropriate amount of water are added to a specific reaction tube, and then the reaction tube is sent into the polymerase amplification device for PCR amplification, and finally analyzed and judged by the detection and analysis module.

[0018] The beneficial effects of the present invention are as follows:

[0019] 1. The device includes a housing, a first fixed plate integrally formed with the housing, an amplification temperature control guide block heating component, a cold storage component, a heat dissipation component, and a driving component. The amplification temperature control guide block is fixedly embedded in the first fixed plate. An air outlet is provided at the bottom of the housing, and a second fixed plate is fixedly arranged around the air outlet. The heat dissipation component is longitudinally and slidably arranged in the second fixed plate. The cold storage component is fixedly installed on the upper part of the heat dissipation component, and a heating component is horizontally and slidably arranged on its upper part. A heat preservation box is also arranged inside the housing. One side of the heat preservation box is open and flush with the heating component. The driving component is used to drive the heating component to slide into the heat preservation box, and at the same time, it can also drive the cold storage component to rise and abut against the amplification temperature control guide block. By providing an air outlet at the bottom of the housing, the accumulated heat generated by the cold storage component during operation can be discharged in time. At the same time, by setting the driving component and the heat preservation box, after the heating component completes heating the amplification temperature control guide block, it can be driven by the driving component to slide into the heat preservation box for heat insulation and preservation to prevent heat from escaping into the housing, solving the problem of heat diffusion and saving the waste heat in the heating component for reuse, thus saving energy. The driving device can not only drive the heating component into the heat preservation shell, but also drive the cold storage component that has completed cold storage to rise and contact the amplification temperature control guide block to quickly cool the amplification temperature control guide block. After the cooling is completed, it can drive the cold storage component to descend, and at the same time, drive the heating component to slide back to the lower part of the amplification temperature control guide block to heat the amplification temperature control guide block, completing the amplification cycle.

[0020] 2. The heat preservation box includes a box body. Third chutes are horizontally arranged on both sides of the box body. A baffle and a second spring are arranged at the bottom of the third chutes. The baffle is fixedly installed at one end of the second spring, and the other end of the second spring is fixedly connected to the bottom of the third chutes. The driving component includes a motor, a screw rod, a gear, and a toothed rod. The motor is fixedly connected to the bottom plate of the housing. The screw rod is fixedly connected to the rotating shaft of the motor. The toothed rod is fixedly installed on both sides of the heating component. The gear is fixedly connected to the top of the screw rod and meshes with the toothed rod. The elastic component composed of the baffle and the second spring enables the heating component to be bounced back by the elastic component even if the heating component moves excessively under the drive of the driving component, so that the toothed rod and the gear mesh again.

[0021] 3. The device also includes a sealing cover component. The sealing cover component includes a cylinder and a heat preservation cover. A sealing cover notch is arranged at the upper part of the open side of the box body. The cylinder is fixedly installed at one end of the second fixed plate close to the box body. The heat preservation cover is fixedly connected to the lower part of the push rod of the cylinder. The heating component includes a first heat conduction plate, a temperature control electric heating plate, and a heat insulation plate. The first heat conduction plate is fixedly connected to the upper side of the temperature control electric heating plate, and the heat insulation plate is fixedly connected to the lower side of the temperature control electric heating plate. After the driving component drives the heating component to slide into the heat preservation box, the cylinder drives the heat preservation cover to cover the first heat conduction plate in the heating component downward. The surrounding structure formed by the heat preservation cover and the heat insulation plate further ensures that the heat in the first heat conduction plate does not escape and is reused, saving energy. Description of the Drawings

[0022] To more clearly illustrate the technical solutions of the embodiments of the present invention, the following will briefly introduce the drawings required for the description of the embodiments. In all the drawings, similar elements or parts are generally identified by similar reference numerals. In the drawings, the elements or parts are not necessarily drawn to actual scale.

[0023] Figure 1 Schematic diagram of the overall structure of the present invention patent;

[0024] Figure 2 Schematic diagram of the section of the present invention patent;

[0025] Figure 3 Schematic diagram of the section of the casing of the present invention patent;

[0026] Figure 4 Schematic diagram of the overall structure of the functional components of the present invention patent;

[0027] Figure 5 Schematic diagram of the overall structure of the heating component of the present invention patent;

[0028] Figure 6 Schematic diagram of the overall structure of the cold storage component of the present invention patent;

[0029] Figure 7 Schematic diagram of the overall structure of the lifting nut of the present invention patent;

[0030] Figure 8 For the present invention patent Figure 4 Enlarged view of location A;

[0031] Figure 9 Schematic diagram of the overall structure of the heat preservation cover of the present invention.

[0032] The reference numerals in the accompanying drawings of the specification include: 1, casing; 101, first fixing plate; 102, air outlet; 103, second fixing plate; 104, first sliding groove; 105, first air inlet; 106, second air inlet; 2, amplification temperature control guide block; 3, heating component; 301, first heat conduction plate; 302, temperature control electric heating plate; 303, heat insulation plate; 4, cold storage component; 401, cold storage box; 402, sealing plate; 403, second heat conduction plate; 404, elastic sealing film; 405, cold conduction sheet; 406, first limiting plate; 407, second sliding groove; 408, second limiting plate; 409, semiconductor refrigeration sheet; 5, heat dissipation component; 501, fan; 502, heat dissipation fin; 503, first slider; 6, driving component; 601, motor; 602, screw rod; 603, nut; 6031, second slider; 6032, first spring; 604, gear; 605, rack; 7, heat preservation box; 701, box body; 702, third sliding groove; 703, baffle; 704, second spring; 705, cover notch; 8, cover component; 801, cylinder; 802, heat preservation cover; 8021, third limiting plate. Detailed implementation manners

[0033] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0034] In the description of the present invention, it should be noted that the orientation or positional relationship indicated by the terms "center", "longitudinal", "transverse", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "top part", "bottom part", "top surface", "bottom surface", "inner", "outer", "inner side", "outer side", etc. is based on the orientation or positional relationship shown in the accompanying drawings. It is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present invention.

[0035] In the description of the present invention, the meaning of "several" is one or more, the meaning of "multiple" is two or more, "greater than", "less than", "exceeding", etc. are understood not to include the number itself, and "above", "below", "within", etc. are understood to include the number itself. If the terms "first", "second", "third" are described, they are only for the purpose of description and distinguishing technical features, and cannot be understood as indicating or implying relative importance or implicitly indicating the quantity of the indicated technical features or implicitly indicating the sequence of the indicated technical features.

[0036] In the description of the present invention, it should be noted that unless otherwise clearly specified and defined, the terms "installation", "connection", "coupling", and "setting" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two components. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances. The embodiments of the present invention will be described below according to its overall structure.

[0037] The present invention provides a polymerase amplification device for detecting African swine fever virus, aiming to solve the problem that the heat dissipation device in the existing polymerase amplification device does not have a well-designed air duct, and the temperature control electric heating plate in the device is also directly exposed inside the box body. After multiple cycles, the heat accumulated inside the box body cannot be discharged, which easily causes damage to the internal components of the device. As Figures 1-9 shown, the device includes a housing 1 and a first fixing plate 101 integrally formed with the housing 1. It further includes an amplification temperature control guide block 2, which is fixedly embedded in the first fixing plate 101. The device also includes a heating component 3, a cold storage component 4, a heat dissipation component 5, and a driving component 6. The heating component 3, the cold storage component 4, and the heat dissipation component 5 are arranged from top to bottom; a square air outlet 102 is opened at the bottom of the housing 1, and a second fixing plate 103 is fixedly arranged around the air outlet 102. The heat dissipation component 5 is longitudinally and limitably slidably arranged in the second fixing plate 103. The cold storage component 4 is fixedly installed on the upper part of the heat dissipation component 5. The heating component 3 is limitably slidably arranged between the amplification temperature control guide block 2 and the cold storage component 4. A heat preservation box 7 is also arranged inside the housing 1. One side of the heat preservation box 7 is open, and the other side is fixedly installed at the rear end of the housing 1. The inner edge of the heat preservation box 7 is larger than the outer edge of the heating component 3, so that the heating component 3 can smoothly slide into the heat preservation box 7. The driving component 6 is fixedly installed on the bottom plate of the housing 1 to drive the heating component 3 to slide into or out of the heat preservation box 7, and at the same time, it can also drive the cold storage component 4 to rise and abut against the amplification temperature control guide block 2.

[0038] As Figure 4As shown in the figure, there are two sets of driving components 6. The two sets of driving components 6 are installed on the bottom plate of the casing 1 and are located on both sides of the second fixing plate 103. The driving component 6 includes a motor 601, a screw rod 602, a nut 603, a gear 604 and a rack 605; there are limiting plates on both sides of the cold storage component 4, namely the first limiting plate 406 and the second limiting plate 408. The first limiting plate 406 and the second limiting plate 408 are in the same vertical plane. Concentric openings are provided on the first limiting plate 406 and the second limiting plate 408. A second chute 407 formed by two ribs is also provided between the first limiting plate 406 and the second limiting plate 408; the motor 601 is fixedly installed on the bottom plate of the casing 1, and the screw rod 602 is fixedly connected to the rotating shaft of the motor 601 and passes through the through holes on the first limiting plate 406 and the second limiting plate 408; the nut 603 is sleeved on the screw rod 602 in a matching manner and is located between the first limiting plate 406 and the second limiting plate 408. A second slider 6031 is provided on the side of the nut 603. The second slider 6031 is longitudinally and limit-slidably arranged in the second chute 407. A first spring 6032 is fixedly provided at the lower part of the nut 603. The first spring 6032 is also sleeved on the screw rod 602. It should be further noted that the rotating shaft of the motor 601 extends out of the second limiting plate 408 and is then fixedly connected to the screw rod 602, and the extending length is greater than the height of the nut 603. The spring is used to bounce the nut 603 back onto the screw rod 602 after the nut 603 moves out of position to cooperate with the rotation of the screw rod 602; the rack 605 is horizontally and fixedly installed on both sides of the heating component 3, and the gear 604 is fixedly connected to the top of the screw rod 602 and meshes with the rack 605.

[0039] During operation, the motor 601 controls the rotation of the screw rod 602, and the gear 604 cooperates with the rack 605. As Figure 4 shown in the figure, the driving heating component 3 slides entirely into the heat preservation box 7 to heat-preserve the first heat conduction plate 301. At the same time, since the nut 603 is limited by the second slider 6031 and cannot rotate, the nut 603 at the bottom of the screw rod 602 slides upward in the second chute 407 along with the rotation of the screw rod 602. After the entire heating component 3 is driven into the heat preservation box 7, the nut 603 reaches the lower part of the first limiting plate 406. The nut 603 drives the cold storage component 4 and the heat dissipation component 5 to rise until the second heat conduction plate 403 contacts the amplification temperature control block 2 to quickly cool down the amplification temperature control block 2.

[0040] By providing an air outlet 102 at the bottom of the housing 1, the device can promptly discharge the accumulated heat generated by the cold storage component 4 during operation. Meanwhile, by providing a driving component 6 and a heat preservation box 7, after the heating component 3 finishes heating the amplification temperature control block 2, it can be driven by the driving component 6 to slide into the heat preservation box 7 for heat insulation and preservation to prevent heat from escaping into the housing 1. This not only solves the problem of heat diffusion but also saves the remaining heat in the heating component 3 for reuse, thus saving energy. The driving device can not only drive the heating component 3 into the heat preservation housing but also drive the cold storage component 4 that has completed cold storage to rise and contact the amplification temperature control block 2 to rapidly cool the amplification temperature control block 2. After the cooling is completed, it can drive the cold storage component 4 to descend, and at the same time drive the heating component 3 to slide back to the lower part of the amplification temperature control block 2 to heat the amplification temperature control block 2, completing the amplification cycle.

[0041] As Figure 5 shown, the heating component 3 includes a first heat conduction plate 301, a temperature control electric heating plate 302, and a heat insulation plate 303. The first heat conduction plate 301 is fixedly connected to the upper side of the temperature control electric heating plate 302 and is made of a material with good thermal conductivity. The heat insulation plate 303 is fixedly connected to the lower side of the temperature control electric heating plate 302 and the heat insulation plate 303 is made of a material with a low thermal conductivity coefficient. Multiple horizontal ribs are provided on the upper part of the first heat conduction plate 301, and corresponding ribs are provided on the lower part of the amplification temperature control block 2. The first heat conduction plate 301 is horizontally limited and slides at the lower part of the amplification temperature control block 2 through the rib fit. The temperature control electric heating plate 302 is used to heat the first heat conduction plate 301, and the heat is transferred from the first heat conduction plate 301 to the amplification temperature control block 2. The setting of the ribs facilitates the horizontal limitation of the first heat conduction plate 301 on one hand, and on the other hand, such a setting increases the contact area between the first heat conduction plate 301 and the amplification temperature control block, accelerating the heat transfer efficiency.

[0042] As Figure 6The cold storage component 4 shown also includes a thermoelectric cooler 409, a cold storage box 401, a sealing plate 402, and a second heat conduction plate 403; the refrigerating side of the thermoelectric cooler 409 is fixedly connected to the bottom of the cold storage box 401 in a sealed manner, and the heating side is fixedly connected to the heat dissipation component 5. While the device is being heated, the thermoelectric cooler 409 operates to fill the cold storage box 401 with a cold storage liquid, and the cold storage liquid is a liquid with a high specific heat capacity. The sealing plate 402 is fixedly connected to the upper part of the cold storage box 401 to seal the cold storage box 401. There are multiple cold conduction plates 405, which are fixedly connected to the lower side of the sealing plate 402 and are in contact with the cold storage liquid, so as to improve the heat transfer efficiency between the cold storage liquid and the sealing plate 402. The second heat conduction plate 403 is fixedly connected to the upper side of the sealing plate 402; to enhance the heat conduction efficiency, silicone grease can be filled between the second heat conduction plate 403 and the sealing plate 402 and between the thermoelectric cooler 409 and the heat dissipation component 5; the heat insulation plate 303 is provided with the same ridges as the lower part of the amplification temperature control block 2, and the second heat conduction plate 403 is provided with the same ridges as the upper part of the first heat conduction plate 301. The heat insulation plate 303 is fitted with the second heat conduction plate 403 through the ridges and is limited to slide on the upper part of the second heat conduction plate 403. It should be further noted that the outermost horizontal ridges of the amplification temperature control block 2 and the heat insulation plate 303 cover the horizontal ridges of the first heat conduction plate 301 and the second heat conduction plate 403 inside, preventing the heat dissipation of the first heat conduction plate 301 from causing energy waste and ensuring that the cold quantity of the second heat conduction plate 403 during the cold storage process is locked and will not dissipate, ensuring energy conservation. The first limiting plate 406, the second limiting plate 408, and the second sliding groove 407 are all arranged on both sides of the cold storage box 401. Temperature sensors for temperature monitoring are respectively arranged in the amplification temperature control block 2, the first heat conduction plate 301, and the second heat conduction plate 403 to monitor the temperatures of various components.

[0043] As Figure 2 shown, the heat dissipation component 5 includes a fan 501, heat dissipation fins 502, and a first slider 503. The fan 501 is fixedly connected to the lower part of the heat dissipation fins 502, the cold storage component 4 is fixedly connected to the upper part of the heat dissipation fins 502, the first slider 503 is arranged at both ends of the heat dissipation fins 502, and the second fixing plate 103 is longitudinally provided with a first sliding groove 104 at both ends. The heat dissipation fins 502 are longitudinally limited to slide in the first sliding groove 104 of the second fixing plate 103 through the first slider 503; a first air inlet 105 and a second air inlet 106 are also arranged on the housing 1. The first air inlet 105 is arranged on both sides of the housing 1, and the second air inlet 106 is arranged on both sides of the second fixing plate 103.

[0044] The thermal insulation box 7 includes a box body 701. One side of the box body 701 is open, and the open side thereof is in contact with the amplification temperature control block 2. Third sliding grooves 702 are horizontally arranged on both sides of the box body 701. The toothed rod 605 is slidably limited in the third sliding grooves 702. A baffle 703 and a second spring 704 are further arranged at the bottom of the third sliding grooves 702. The baffle 703 is fixedly installed at one end of the second spring 704, and the other end of the second spring 704 is fixedly connected to the bottom of the third sliding grooves 702. The elastic component composed of the baffle 703 and the second spring 704 enables the heating component 3 to be bounced back by the elastic component even if the heating component 3 moves excessively under the drive of the drive component 6, so that the toothed rod 605 meshes with the gear 604 again.

[0045] Openings are arranged on both sides of the cold storage box 401, and elastic sealing films 404 are hermetically arranged at the openings. Through the deformation of the elastic sealing films 404, the internal pressure change of the cold storage box 401 is weakened, thereby reducing the liquid leakage probability between the cold storage box 401 and the sealing plate 402 and the semiconductor refrigeration sheet 409.

[0046] In a preferred embodiment, the device further includes a capping component 8. The capping component 8 includes a cylinder 801 and a thermal insulation cover 802. A capping notch 705 is arranged at the upper part of the open side of the box body 701. The cylinder 801 is fixedly installed at one end of the second fixing plate 103 close to the box body 701. The thermal insulation cover 802 is fixedly connected to the lower part of the push rod of the cylinder 801. A third limiting plate 8021 protrudes from the upper side of the thermal insulation cover 802. The materials of the thermal insulation cover 802 and the box body 701 of the thermal insulation box 7 are both materials with low thermal conductivity coefficients. The lower part of the thermal insulation cover 802 is wedge-shaped, ensuring that the hypotenuse of the thermal insulation board when the thermal insulation cover 802 descends pushes the first heat conduction plate 301 into the thermal insulation box 7 to prevent interference. After the drive component 6 drives the heating component 3 to slide into the interior of the thermal insulation box 7, the cylinder 801 drives the thermal insulation cover 802 to cover the first heat conduction plate 301 in the heating component 3 downward. The surrounding structure formed by the thermal insulation cover 802 and the heat insulation board 303 further ensures that the heat in the first heat conduction plate 301 does not escape and is reused, saving energy.

[0047] The present invention also provides a system for detecting African swine fever virus, including the above-mentioned polymerase amplification device for detecting African swine fever virus, as well as a sample processing module, a PCR reaction system configuration and detection and analysis module. The sample is first processed by the sample processing module to extract the African swine fever virus nucleic acid therein, and then the PCR reaction system is configured. The extracted African swine fever virus nucleic acid, primers, deoxynucleoside triphosphates, buffer solution, heat-resistant DNA polymerase and an appropriate amount of water are added to a specific reaction tube, and then the reaction tube is sent into the polymerase amplification device for PCR amplification, and finally analyzed and judged by the detection and analysis module.

[0048] When the present device is in use, the reaction tube after the PCR reaction system is configured is inserted into the amplification temperature control block 2, asFigure 2 As shown, denaturation treatment is first carried out. It is heated by a temperature-controlled electric hot plate 302, and the heat is transferred to the amplification temperature control block 2 through the first heat conduction plate 301, so that the amplification temperature control block 2 is heated up. The temperature is monitored and controlled by the temperature sensor in the amplification temperature control block 2. At the same time, the operation of the semiconductor refrigeration sheet 409 can be controlled for pre-cooling, and the cold is stored through the cold storage liquid inside the cold storage box 401. The refrigeration of the semiconductor refrigeration sheet 409 reduces the temperature of the cold storage liquid inside the cold storage chamber. Through the conduction of the cold conduction sheet 405 and the sealing plate 402, the second heat conduction plate 403 is in a low-temperature state. At this time, the heat insulation plate 303 will cover and seal the second heat conduction plate 403, reducing the contact area between the second heat conduction plate 403 and the flowing air, thereby reducing the loss of cold quantity at the end of the second heat conduction plate 403.

[0049] When the denaturation treatment is completed, annealing and cooling are required. At this time, the motor 601 controls the rotation of the screw 602, and the gear 604 cooperates with the rack 605, as Figure 4 As shown, the heating component 3 is driven to slide into the heat preservation box 7 as a whole to keep the first heat conduction plate 301 warm. After the heating component 3 enters the heat preservation box 7 as a whole, the air cylinder 801 drives the heat preservation cover 802 to cover the first heat conduction plate 301 in the heating component 3 downward. The surrounding structure formed by the heat preservation cover 802 and the heat insulation plate 303 further ensures that the heat in the first heat conduction plate 301 does not escape and is reused. At the same time, because the nut 603 is limited by the second slider 6031 and cannot rotate, the nut 603 at the bottom of the screw 602 slides upward in the second chute 407 with the rotation of the screw 602. After the heating component 3 is driven into the heat preservation box 7 as a whole, the nut 603 reaches the lower part of the first limiting plate 406, and the nut 603 drives the cold storage component 4 and the heat dissipation component 5 to rise until the second heat conduction plate 403 contacts the amplification temperature control block 2 to quickly cool the amplification temperature control block 2. Due to the limitation of the second fixing plate 103 and the settings of the first air inlet 105 and the second air inlet 106, the heat generated by the cold storage component 4 can be continuously discharged from the air outlet 102.

[0050] Next, the next cycle is required, and the reaction tube is denatured again. At this time, the motor 601 rotates in reverse, the nut 603 descends, driving the entire cold storage component 4 to descend. After the nut 603 descends to the lowest part of the screw 602, the nut 603 will fall off the screw 602 as the screw 602 rotates. However, the first spring 6032 fixed to the lower part of the nut 603 will bounce the nut 603 back due to the limit of the second limit plate 408, preventing the nut 603 from detaching from the screw 602. After the cold storage component 4 descends to the lowest point, the cylinder 801 drives the heat preservation cover 802 to rise. Since the elastic component composed of the second spring 704 and the third limit plate 8021 is compressed, the accumulated elastic potential energy will bounce the heating component 3 back, causing the toothed rod 605 to mesh with the gear 604 again. The gear 604 drives the toothed rod 605 to drive the heating component 3 to slide back under the amplification temperature control guide block 2 again to heat it. Such denaturation and annealing are repeated to amplify the specific DNA in the reaction tube.

[0051] During this process, the heat preservation cover 802 and the heat preservation box 7 can preserve the heat in the first heat conduction plate 301 as much as possible. At the same time, the heat generated by the cold storage component 4 is continuously discharged by the heat dissipation component 5 from the air outlet 102. Therefore, a relatively stable temperature can be maintained inside the device to ensure the working environment of each internal component and prevent each component from being damaged due to high temperature.

[0052] The above description of the specific exemplary embodiments of the present invention is for the purpose of illustration and exemplification. These descriptions are not intended to limit the present invention to the precise forms disclosed. Obviously, many changes and variations can be made according to the above teachings. Although the embodiments of the present invention have been shown and described, the specific embodiments are only explanations of the present invention and not limitations of the invention. The specific features, structures, materials or characteristics described can be combined in a suitable manner in any one or more embodiments or examples. The purpose of selecting and describing the exemplary embodiments is to explain the specific principles of the present invention and its practical applications, so that those skilled in the art can, after reading this specification, make modifications, substitutions, variations and various different selections and changes that do not make creative contributions to the embodiments as needed, but as long as they are within the scope of the claims of the present invention, they are protected by the patent law.

Claims

1. A polymerase amplification device for detecting African swine fever virus, comprising a machine shell (1), a first fixing plate (101) integrally formed with the machine shell (1), and an amplification temperature control guide block (2), wherein the amplification temperature control guide block (2) is fixedly embedded in the first fixing plate (101), and is characterized in that: The device further includes a heating component (3), a cold storage component (4), a heat dissipation component (5) and a driving component (6). The heating component (3) is arranged in a limited and sliding manner below the amplification temperature control guide block (2). An air outlet (102) is formed at the bottom of the housing (1), and a second fixing plate (103) is fixedly arranged around the air outlet (102). The heat dissipation component (5) is longitudinally arranged in a limited and sliding manner within the second fixing plate (103). The cold storage component (4) is fixedly installed on the upper part of the heat dissipation component (5), and the heating component (3) is horizontally arranged in a limited and sliding manner on its upper part. A heat preservation box (7) is further arranged inside the housing (1). One side of the heat preservation box (7) is open and flush with the heating component (3). The driving component (6) is installed at the bottom of the housing (1) to drive the heating component (3) to slide into the heat preservation box (7), and at the same time, it can also drive the cold storage component (4) to rise and abut against the amplification temperature control guide block (2). There are two groups of the driving components (6). The two groups of the driving components (6) are installed on the bottom plate of the housing (1) and on both sides of the second fixing plate (103). The driving component (6) includes a motor (601), a screw rod (602), a nut (603), a gear (604) and a rack (605). Two groups of a first limiting plate (406) and a second limiting plate (408) are arranged on both sides of the cold storage component (4). The first limiting plate (406) and the second limiting plate (408) are in the same vertical plane and a second sliding groove (407) is further arranged between them. The motor (601) is fixedly connected to the bottom plate of the housing (1). The screw rod (602) is fixedly connected to the rotating shaft of the motor (601) and passes through the first limiting plate (406) and the second limiting plate (408). The nut (603) is sleeved on the screw rod (602) and is located between the first limiting plate (406) and the second limiting plate (408). A second sliding block (6031) is arranged on the side of the nut (603). The second sliding block (6031) is longitudinally arranged in a limited and sliding manner within the second sliding groove (407). A first spring (6032) is further fixedly connected to the bottom of the nut (603). The rack (605) is fixedly installed on both sides of the heating component (3). The gear (604) is fixedly connected to the top of the screw rod (602) and meshes with the rack (605).

2. The polymerase amplification device for detecting African swine fever virus according to claim 1, wherein: The heating component (3) includes a first heat conduction plate (301), a temperature-controlled electric heating plate (302), and a heat insulation plate (303). The first heat conduction plate (301) is fixedly connected to the upper side of the temperature-controlled electric heating plate (302), and the heat insulation plate (303) is fixedly connected to the lower side of the temperature-controlled electric heating plate (302). The first heat conduction plate (301) is horizontally limited and slidable at the lower part of the amplification temperature control guide block (2). The cold storage component (4) further includes a semiconductor refrigeration chip (409), a cold storage box (401), a sealing plate (402), a cold conduction plate (405), and a second heat conduction plate (403). The refrigerating side of the semiconductor refrigeration chip (409) is closely attached to the bottom of the cold storage box (401), and the heating side is fixedly connected to the heat dissipation component (5). The cold storage box (401) is filled with a cold storage liquid. The sealing plate (402) is closely attached to the upper part of the cold storage box (401). There are multiple cold conduction plates (405) fixedly connected to the lower side of the sealing plate (402) and in contact with the cold storage liquid. The second heat conduction plate (403) is fixedly connected to the upper side of the sealing plate (402). The heat insulation plate (303) is limited and slidably arranged on the upper part of the second heat conduction plate (403). The first limiting plate (406), the second limiting plate (408), and the second sliding groove (407) are all arranged on both sides of the cold storage box (401). Temperature sensors for temperature monitoring are respectively arranged in the amplification temperature control guide block (2), the first heat conduction plate (301), and the second heat conduction plate (403).

3. The polymerase amplification device for detecting African swine fever virus according to claim 1, wherein: The heat dissipation component (5) includes a fan (501), heat dissipation fins (502), and a first slider (503). The fan (501) is fixedly connected to the lower part of the heat dissipation fins (502), and the cold storage component (4) is fixedly connected to the upper part of the heat dissipation fins (502). The first slider (503) is arranged at both ends of the heat dissipation fins (502). First sliding grooves (104) are longitudinally arranged at both ends of the second fixing plate (103). The heat dissipation fins (502) are longitudinally limited and slidably arranged in the first sliding grooves (104) of the second fixing plate (103) through the first slider (503).

4. The polymerase amplification device for detecting African swine fever virus according to claim 1, wherein: A first air inlet (105) and a second air inlet (106) are further arranged on the machine shell (1). The first air inlet (105) is arranged on both sides of the machine shell (1), and the second air inlet (106) is arranged on both sides of the second fixing plate (103).

5. The polymerase amplification device for African swine fever virus detection according to claim 1, wherein: The heat preservation box (7) includes a box body (701). One side of the box body (701) is open, and the open side abuts against the amplification temperature control guide block (2). Third sliding grooves (702) are horizontally arranged on both sides of the box body (701). The toothed rod (605) is limited and slidable in the third sliding grooves (702). A baffle (703) and a second spring (704) are further arranged at the bottom of the third sliding grooves (702). The baffle (703) is fixedly installed at one end of the second spring (704), and the other end of the second spring (704) is fixedly connected to the bottom of the third sliding grooves (702).

6. The polymerase amplification device for detecting African swine fever virus according to claim 2, wherein: The cold storage box (401) is provided with openings on both sides, and elastic sealing films (404) are hermetically arranged at the openings.

7. The polymerase amplification device for detecting African swine fever virus according to claim 5, characterized in that: It further includes a cover component (8), the cover component (8) includes a cylinder (801) and a heat preservation cover (802), an upper part of one side of the opening of the box body (701) is provided with a cover notch (705), the cylinder (801) is fixedly installed at one end of the second fixed plate (103) close to the box body (701), the heat preservation cover (802) is fixedly connected to the lower part of the push rod of the cylinder (801), and a third limiting plate (8021) protrudes from the upper side of the heat preservation cover (802).

8. The polymerase amplification device for detecting African swine fever virus according to claim 7, wherein: Both the heat preservation cover (802) and the box body (701) of the heat preservation box (7) are made of materials with low heat conduction coefficients.

9. The polymerase amplification device for detecting African swine fever virus according to claim 7, characterized in that: The lower part of the heat preservation cover (802) is wedge-shaped.

10. A system for detecting African swine fever virus, characterized in that: It includes the polymerase amplification device for African swine fever virus detection, the sample processing module, the PCR reaction system configuration and the detection and analysis module according to any one of claims 1-9. The sample is first processed by the sample processing module to extract the African swine fever virus nucleic acid therein, and then the PCR reaction system is configured. The extracted African swine fever virus nucleic acid, primers, deoxynucleoside triphosphates, buffer solution, heat-resistant DNA polymerase and an appropriate amount of water are added to a specific reaction tube, and then the reaction tube is sent into the polymerase amplification device for PCR amplification, and finally analyzed and judged by the detection and analysis module.

Images