Artificial pollution solution for green algae as well as preparation method and application of artificial pollution solution

Abstract

The invention belongs to the technical field of insulator pollution control, and particularly relates to an artificial pollution solution for green algae and a preparation method and application thereof. The green alga artificial contamination solution provided by the invention comprises the components such as the green alga culture medium, the green alga concentrated solution, the artificial contamination particles and the nonionic surfactant solution, so that attachment of microbial communities on the surface of an insulator can be simulated more truly, and a more accurate experimental basis is provided for research on a green alga contamination mechanism; the method meets the requirements of research and development of an insulator efficient cleaning technology, can shorten the experimental period, and has high practicability, so that the technical problem that in the prior art, a green alga culture medium is difficult to serve as a green alga artificial staining solution and meets the requirements of research and development of the insulator efficient cleaning technology is solved.

Description

Technical Field

[0001] This application belongs to the technical field of insulator pollution control, and particularly relates to an artificial green algae contamination solution, a preparation method thereof, and an application thereof. Background Art

[0002] In modern power systems, transmission lines mainly include electrical equipment such as conductors, towers, insulators, fittings, grounding devices, and lightning conductors; among them, insulators are key components to ensure the normal operation of transmission lines. Their main function is to provide electrical isolation and mechanical support. However, insulators in transmission lines are exposed to the natural environment for a long time. In areas with rainy and humid climates in the south, suitable sunlight and temperature will promote the reproduction of microorganisms such as green algae. Research shows that when microorganisms such as green algae attach to the surface of insulators in a humid environment and multiply in large numbers, forming a biofilm with strong adhesion, it will reduce the hydrophobicity of the insulator surface, resulting in the formation of a water film on the surface, changing the surface electric field distribution, and ultimately increasing the risk of flashover, discharge, and even breakdown of the equipment. It can be seen that the pollution of microorganisms such as green algae on the insulator surface poses a major threat to the safe operation of transmission lines.

[0003] At present, researchers will improve the design or material of insulators, such as using anti-flashover coatings or self-cleaning materials, to reduce the pollution of microorganisms such as green algae on the insulator surface; however, the prerequisite for developing high-efficiency cleaning technologies such as self-cleaning materials for insulators is to simulate the natural attachment process of green algae on the insulator surface through experiments. However, there are multiple problems to be solved in the experimental simulation of green algae pollution technology. For example, in the natural state, the pollution accumulation rate of microorganisms such as green algae is slow, resulting in a long experimental period and difficulty in meeting the research requirements; when using a culture medium inoculated with microorganisms such as green algae as an artificial green algae contamination solution to promote the attachment of microorganisms such as green algae on the insulator surface, due to the single formula of traditional culture media for microorganisms such as green algae, it is impossible to comprehensively simulate the complex nutritional conditions and physical and chemical environments in the natural environment, insufficient consideration of the growth-promoting factors of green algae, and ignoring the true composition of the attachments on the insulator surface, resulting in deviations between the cultured green algae in terms of adhesion and growth behavior and the actual situation, limiting the practical guiding significance of experimental results; therefore, the existing culture media inoculated with microorganisms such as green algae as artificial green algae contamination solutions are difficult to meet the requirements for developing high-efficiency cleaning technologies for insulators. Summary of the Invention

[0004] In view of this, this application provides an artificial green algae contamination solution, a preparation method thereof, and an application thereof, which are used to solve the technical problem that the existing green algae culture medium is difficult to be used as an artificial green algae contamination solution to meet the requirements for developing high-efficiency cleaning technologies for insulators.

[0005] In a first aspect of this application, an artificial green algae contamination solution includes the following components: a green algae culture medium, a green algae concentrate, artificial pollution particles, and a non-ionic surfactant solution;

[0006] The artificial pollution particles are diatomite / sodium chloride composite particles.

[0007] Preferably, the non-ionic surfactant solution is selected from at least one of polyvinylpyrrolidone solution, fatty alcohol polyoxyethylene ether, alkylphenol polyoxyethylene ether, and fatty acid polyoxyethylene ester.

[0008] Preferably, in the artificial pollution solution of green algae, the volume mass ratio of the green algae culture medium, green algae concentrated solution, polyvinylpyrrolidone, and artificial pollution particles polyvinylpyrrolidone solution is 500 - 1500 mL: 50 - 150 mL: 50 - 150 mL: 5 - 15 g. Preferably, the mass ratio of diatomite to sodium chloride in the artificial pollution particles is 3 - 9:1;

[0009] The concentration of the non-ionic surfactant solution is 2 - 10 mg / L.

[0010] Preferably, in 500 - 1500 mL of the green algae culture medium, it includes NaNO3, K2HPO4·3H2O, MgSO4·7H2O, CaCl2·2H2O, KH2PO4, NaCl, Na2CO3, and FeCl3·6H2O, Fe-EDTA solution, trace element solution, soil extract, jackfruit leaf extract, and cow ear maple leaf extract;

[0011] The solute in the trace element solution includes H3BO3, ZnSO4·7H2O, MnCl2·4H2O, CuSO4·5H2O, and (NH4)6Mo7O2·4H2O.

[0012] Preferably, in 500 - 1500 mL of the green algae culture medium, it includes 0.1 - 0.5 g of NaNO3, 0.05 - 0.1 g of K2HPO4·3H2O, 0.05 - 0.1 g of MgSO4·7H2O, 0.01 - 0.05 g of CaCl2·2H2O, 0.1 - 0.25 g of KH2PO4, 0.01 - 0.05 g of NaCl, 1 - 3 g of Na2CO3, and 0.001 - 0.01 g of FeCl3·6H2O, 0.5 - 2 mL of Fe-EDTA solution with a concentration of 1 - 5 mmol, 0.5 - 2 mL of trace element solution, 20 - 60 mL of soil extract, 5 - 20 mL of jackfruit leaf extract, 5 - 20 mL of cow ear maple leaf extract;

[0013] In 0.5 - 2 mL of the trace element solution, the solute includes 1 - 5 mg of H3BO3, 0.1 - 0.5 mg of ZnSO4·7H2O, 1 - 3 mg of MnCl2·4H2O, 0.05 - 0.1 mg of CuSO4·5H2O, and 0.02 - 0.06 mg of (NH4)6Mo7O2·4H2O.

[0014] Preferably, the green algae culture medium includes 0.25 g of NaNO3, 0.075 g of K2HPO4·3H2O, 0.075 g of MgSO4·7H2O, 0.025 g of CaCl2·2H2O, 0.175 g of KH2PO4, 0.025 g of NaCl, 2 g of Na2CO3, 0.005 g of FeCl3·6H2O, 1 mL of a 3 mmol Fe-EDTA solution, 1 mL of a trace element solution, 40 mL of a soil extract, 10 mL of a jackfruit leaf extract, and 10 mL of a cow's ear maple leaf extract;

[0015] The solutes in the 1 mL of trace element solution include 2.86 mg of H3BO3, 0.22 mg of ZnSO4·7H2O, 1.81 mg of MnCl2·4H2O, 0.079 mg of CuSO4·5H2O, and 0.039 mg of (NH4)6Mo7O2·4H2O.

[0016] In a second aspect of the present application, a method for preparing an artificial polluted solution of green algae can prepare the artificial polluted solution of green algae described in the first aspect. The preparation method includes the following steps:

[0017] Step S1: Mix the weighed NaNO3, K2HPO4·3H2O, MgSO4·7H2O, CaCl2·2H2O, KH2PO4, NaCl, Na2CO3, FeCl3·6H2O, Fe-EDTA solution, trace element solution, soil extract, jackfruit leaf extract, cow's ear maple leaf extract, and distilled water evenly, and sterilize to obtain a green algae culture medium;

[0018] Step S2: Inoculate the green algae concentrate into the green algae culture medium for cultivation to obtain a green algae suspension;

[0019] Step S3: Add artificial pollution particles to the green algae suspension and disperse them to obtain an artificial polluted suspension of green algae;

[0020] Step S4: Add a non-ionic surfactant solution to the artificial polluted suspension of green algae and mix evenly to obtain an artificial polluted solution of green algae.

[0021] Preferably, in step S1, the preparation method of the Fe-EDTA solution includes: dissolving Na2EDTA in distilled water as solution A, dissolving FeCl3·6H2O in HCl solution as solution B, and mixing and stirring solution A and solution B evenly to obtain the Fe-EDTA solution;

[0022] The preparation method of the trace element solution includes: dissolving H3BO3, ZnSO4·7H2O, MnCl2·4H2O, CuSO4·5H2O, and (NH4)6Mo7O2·4H2O in distilled water to obtain the trace element solution;

[0023] The preparation method of the soil extract includes: adding distilled water and unfertilized soil into a container, sealing the mouth of the container with a breathable plug, successively performing boiling water heating, cooling, precipitation, and filtration, and taking the supernatant of the filtrate for sterilization to obtain the soil extract;

[0024] The preparation method of the jackfruit leaf extract includes: adding distilled water to fresh jackfruit leaves, successively performing heating and boiling, cooling, and filtration, and taking the supernatant of the filtrate for sterilization to obtain the jackfruit leaf extract;

[0025] The preparation method of the earleaf maple leaf extract includes: adding distilled water to earleaf maple leaf powder, successively performing heating and boiling, cooling, and filtration, and taking the supernatant of the filtrate for sterilization to obtain the earleaf maple leaf extract.

[0026] Preferably, in step S2, the light intensity for the cultivation is 4000 - 6000 lux;

[0027] The light cycle for the cultivation is 12 hours of light and 12 hours of darkness successively;

[0028] The temperature for the cultivation is 20 - 30 °C;

[0029] The pH value for the cultivation is 6.5 - 8.0.

[0030] Preferably, in step S3, the preparation method of the artificial contamination particles includes: mixing diatomite particles, solid sodium chloride particles, and deionized water evenly, evaporating the water, and performing grinding after evaporation to obtain the artificial contamination particles.

[0031] The third aspect of the present application provides an application of a green algae artificial contamination solution in the research and development of an efficient insulator cleaning technology.

[0032] Compared with the prior art, the green algae artificial contamination solution provided by the present application has at least the following beneficial effects:

[0033] 1. In the green algae artificial contamination solution provided by the present application, it includes a green algae concentrate providing the algal source, a green algae culture medium simulating the nutrient conditions and physical and chemical environment on the surface of insulators in the natural environment, artificial contamination particles, and artificial biofilm components such as polyvinylpyrrolidone solution. It can simulate the natural attachment process of green algae on the surface of insulators in experiments, provide a more accurate experimental basis for the research on the green algae pollution mechanism, and meet the requirements for the research and development of an efficient insulator cleaning technology.

[0034] 2. The green algae culture medium in the artificial contamination solution of green algae provided by this application includes nitrogen source, phosphorus source, magnesium source, calcium source, sodium source, carbon source, iron source, trace elements such as boron, zinc, manganese, copper and molybdenum, special trace elements and organic substances in the soil, and various plant growth promoting substances, which can promote the growth and metabolic activities of green algae, shorten the attachment process of simulating green algae on the insulator surface in the experiment, and accelerate the experimental process.

[0035] 3. The main components used in the artificial contamination solution of green algae provided by this application are natural plant extracts and environmentally friendly materials, meeting the requirements of modern environmental protection and sustainable development. Description of the Drawings

[0036] In order to more clearly illustrate the specific embodiments of this application or the technical solutions in the prior art, the following will briefly introduce the drawings required for use in the description of the specific embodiments or the prior art. Obviously, the drawings in the following description are some embodiments of this application. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0037] Figure 1 It is a schematic diagram of the formula of an artificial contamination solution of green algae provided in Embodiment 1 of this application;

[0038] Figure 2 It is a schematic flowchart of the preparation method of an artificial contamination solution of green algae provided in Embodiment 1 of this application. Detailed Embodiments

[0039] This application provides an artificial contamination solution of green algae, its preparation method and application, which are used to solve the technical problem that it is difficult for the green algae culture medium in the prior art to be used as an artificial contamination solution of green algae to meet the requirements of developing efficient insulator cleaning technology.

[0040] The following will clearly and completely describe the technical solutions of this application with reference to the drawings. Obviously, the described embodiments are some embodiments of this application, rather than all embodiments. Based on the embodiments in this application, all other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the protection scope of this application.

[0041] In view of the current defects of the green algae culture medium, which is difficult to comprehensively simulate the complex nutritional conditions and physical and chemical environment in the natural environment, with insufficient consideration of the factors promoting the growth of green algae and ignoring the true composition of the attachments on the insulator surface, resulting in difficulty in meeting the requirements of developing efficient insulator cleaning technology; this application provides an artificial contamination solution of green algae; the composition of the artificial contamination solution of green algae provided by this application includes: green algae culture medium, green algae concentrate, artificial contamination particles, and non-ionic surfactant solutions such as polyvinylpyrrolidone solution; the artificial contamination particles are diatomite / sodium chloride composite particles.

[0042] In the artificial green algae contamination solution provided by this application, the concentrated green algae solution is a commercially available conventional Chlorella liquid algal species, which is used to provide the algal source. The green algae culture medium includes various inorganic salts, trace elements, and organic substances, which can comprehensively simulate the nutrient conditions and physicochemical environment in the natural environment and promote the growth and reproduction of green algae. At the same time, insulators are applied to transmission lines. When the transmission lines supply power to industrial and mining enterprises, industrial mineral powders are likely to adhere to the surface of the insulators, and in the humid substrate of the southern coast, the salts brought by the sea breeze will also adhere to the surface of the insulators, resulting in a complex attachment of microorganisms on the surface of the insulators. In this application, diatomite / sodium chloride composite particles, including mineral impurities and salts such as silicon dioxide, aluminum oxide, iron oxide, and calcium oxide, are introduced into the artificial green algae contamination solution, which can simulate the real situation of microorganism attachment on the surface of the insulators. In addition, polyvinylpyrrolidone solution, polyoxyethylene solutions such as fatty alcohol polyoxyethylene ether, are all non-ionic surfactant solutions. They have an affinity for the particle surface and also have an affinity for the solvent, simulating the formation of green algae biofilms in the natural environment and more realistically simulating the attachment of microorganism communities on the surface of the insulators. Therefore, the artificial green algae contamination solution provided by this application has strong practicability. Components such as the green algae culture medium can shorten the experimental period, while components such as artificial contamination particles and non-ionic surfactant solutions can simulate the natural attachment process of green algae on the surface of the insulators in the experiment, providing a more accurate experimental basis for the study of the green algae pollution mechanism and meeting the requirements for developing efficient insulator cleaning technologies.

[0043] Preferably, in the artificial green algae contamination solution provided by the present application, the green algae culture medium includes NaNO3, K2HPO4·3H2O, MgSO4·7H2O, CaCl2·2H2O, KH2PO4, NaCl, Na2CO3, FeCl3·6H2O, Fe-EDTA solution, trace element solution, soil extract, jackfruit leaf extract, and cow ear maple leaf extract; among them, NaNO3 serves as a nitrogen source for the synthesis of amino acids and nucleic acids by green algae; K2HPO4·3H2O provides a phosphorus source for green algae for the synthesis of nucleic acids, ATP, and phospholipids; MgSO4·7H2O serves as a magnesium source, which is a key element for photosynthesis in green algae cells; CaCl2·2H2O serves as a calcium source, which plays a role in the formation of cell walls and the conduction of cell signals; KH2PO4 is also used to provide a phosphorus source and regulate the pH value of the culture medium; NaCl provides a sodium source for the culture medium, and Na2CO3 serves as a carbon source and can also regulate the pH value of the culture medium. FeCl3·6H2O serves as an iron source, which is a cofactor of various enzymes and is used for photosynthesis and respiration. The Fe-EDTA solution can increase the solubility and bioavailability of iron. Elements such as boron, zinc, manganese, copper, and molybdenum in the trace element solution are crucial for the growth and metabolic activities of green algae. In addition, the soil extract can also simulate soil conditions in the natural environment and provide special trace elements and organic substances in the soil. At the same time, the jackfruit leaf extract and cow ear maple leaf extract contain various plant growth-promoting substances, including growth hormones and trace elements. These components can promote the growth and reproduction of green algae, increase the biomass of algae, and the compounds contained can enhance the stress resistance of green algae such as ultraviolet resistance and antioxidant ability.

[0044] Next, a specific description will be given to an artificial green algae contamination solution provided by the present application in combination with examples and experimental examples.

[0045] Example 1

[0046] The present application provides a preparation method of an artificial green algae contamination solution in Example 1. The preparation method includes the following steps: the preparation step of the green algae culture medium, the step of culturing algae, the step of adding artificial pollution particles, the step of adding artificial biofilm components, and the dilution step.

[0047] The preparation step of the green algae culture medium includes:

[0048] Weigh 10 mL of the extract of Niu'er maple leaves, 10 mL of the extract of jackfruit leaves, 40 mL of the soil extract, 1 mL of the trace element solution, 1 mL of the Fe-EDTA solution, 0.25 g of NaNO3, 0.075 g of K2HPO4·3H2O, 0.075 g of MgSO4·7H2O, 0.025 g of CaCl2·2H2O, 0.175 g of KH2PO4, 0.025 g of NaCl, 2 g of Na2CO3, and 0.005 g of FeCl3·6H2O as the raw materials for the green algae culture medium;

[0049] Add the weighed 1 mL of Fe-EDTA solution, 0.25 g of NaNO3, 0.075 g of K2HPO4·3H2O, 0.075 g of MgSO4·7H2O, 0.025 g of CaCl2·2H2O, 0.175 g of KH2PO4, 0.025 g of NaCl, 2 g of Na2CO3, and 0.005 g of FeCl3·6H2O into a beaker, add a small amount of distilled water, stir with a magnetic stirrer until completely dissolved, then add 10 mL of the extract of Niu'er maple leaves, 10 mL of the extract of jackfruit leaves, 40 mL of the soil extract, 1 mL of the trace element solution, and 1 mL of the Fe-EDTA solution. After mixing all the components, add distilled water to make up to 1000 mL. Subsequently, use an autoclave to sterilize at 121 °C for 30 minutes to obtain the green algae culture medium.

[0050] The steps for culturing algae include: Inoculate 100 mL of the purchased green algae concentrate (Chlorella liquid algal species) into the sterilized culture medium under sterile conditions, and use a spreading rod to spread the bacterial liquid evenly. Use an artificial light source, control the light intensity at 4000 - 6000 lux, maintain a 12-hour light / 12-hour dark cycle, set the temperature at 20 - 30 °C, and keep the pH value between 6.5 - 8.0.

[0051] The steps for adding artificial fouling particles include: First, put 30 g of diatomaceous earth and 5 g of solid sodium chloride particles into a glass beaker according to a mass ratio of 6:1, add 750 g of deionized water, and stir well with a glass rod; then place the beaker in a constant temperature test box and place it at 80 °C for 6 h. After the water has evaporated, take out the beaker; next, pour the mixture of diatomaceous earth and sodium chloride in the beaker into a mortar, and grind it thoroughly to obtain artificial fouling particles; then add 10 g of the prepared artificial fouling particles to the green algae suspension, and use equipment such as a magnetic stirrer or an ultrasonic disperser to disperse it evenly.

[0052] The steps for adding the artificial biofilm component include: Use 100 mL of a 5 mg / L polyvinylpyrrolidone (PVP) solution as the artificial biofilm component, add it to the green algae suspension and mix evenly to obtain the artificially contaminated green algae solution.

[0053] The dilution step includes: diluting the artificial contaminated solution of green algae and deionized water according to a volume ratio of 1:2 as needed, and adjusting the pH value to 7.5 using NaOH / HCl.

[0054] In this embodiment, the preparation steps of 10 mL of Liquidambar formosana leaf extract include: taking 200 g of fresh Liquidambar formosana leaves, cleaning, drying or baking them, and then grinding them into powder. Mix the powder with distilled water and heat it in boiling water. The amount of distilled water used is 10 times the mass of the leaves, and the heating temperature ranges from 90 °C to 100 °C, and continue to boil for 3 hours. After boiling, cool it, filter to obtain the supernatant, and sterilize it under high pressure at 121 °C for 30 minutes. After sterilization, store it, and take 10 mL of it for the preparation of the contaminated solution.

[0055] The preparation steps of 10 mL of Artocarpus heterophyllus leaf extract include: taking 200 g of fresh Artocarpus heterophyllus leaves, the amount of distilled water used is 8 times the mass of the leaves, and keep the heating temperature in the range of 90 °C to 100 °C. Continue to boil for 3 hours. After boiling, cool it, filter to obtain the supernatant, and sterilize it under high pressure at 121 °C for 30 minutes. After sterilization, store it, and take 10 mL of it for the preparation of the contaminated solution.

[0056] The preparation steps of 40 mL of soil extract include: taking 200 g of unfertilized soil, adding 1000 mL of distilled water, sealing the bottle mouth with a breathable plug, heating it in boiling water in a water bath for 3 hours, cooling it, and precipitating for 24 hours. This process is carried out continuously for 3 times, then filter it, take the supernatant, sterilize it in an autoclave and store it, and take 40 mL of it for the preparation of the contaminated solution

[0057] The preparation steps of 1 mL of trace element solution include: weighing 286 mg of H3BO3, 22 mg of ZnSO4·7H2O, 181 mg of MnCl2·4H2O, 7.9 mg of CuSO4·5H2O, and 3.9 mg of (NH4)6Mo7O2·4H2O and dissolving them in 100 mL of distilled water, and taking 1 mL of it for the preparation of the contaminated solution.

[0058] The preparation steps of 1 mL of Fe-EDTA solution include: dissolving 1 g of Na2EDTA in 50 mL of distilled water as solution A, dissolving 81 mg of FeCl3·6H2O in 50 mL of 0.1 mol / L HCl solution as solution B. After fully stirring and dissolving, mix solutions A and B and stir evenly.

[0059] In the embodiments of the present application, after culturing algae in an algal medium, if only a part of the algal suspension is used, a part of the green algae can be dried from an average water content of 80% wet basis to a safe water content of 10%. The drying can be carried out in the temperature range of 40-140°C, and the optimal medium temperature is 60-80°C to avoid adverse effects on the surface color, structure, and carbohydrate and lipid composition of the green algae. Rapid freezing is carried out at a very low temperature (about -70°C), and then vacuum drying is carried out at a very low temperature to keep the metabolic activities of the algal species in the algal suspension in a highly quiescent state.

[0060] Comparative Example 1

[0061] Comparative Example 1 of the present application provides a method for preparing an artificial contaminated solution of green algae. The preparation method includes the following steps of preparing an algal medium, culturing algae, adding artificial fouling particles, and diluting; different from Example 1, polyvinylpyrrolidone (PVP) is not added as an artificial biofilm component.

[0062] Experimental Example 1

[0063] In Experimental Example 1 of the present application, performance tests are carried out on the artificial contaminated solutions of green algae provided by Example 1 and Comparative Example 1. The performance test is the growth and attachment of green algae on the surface of the insulator. During the performance test, the artificial contaminated solution of green algae provided by Example 1 is used as the experimental group, and the artificial contaminated solution of green algae provided by Comparative Example 1 is used as the control group. The performance test results are shown in Table 1.

[0064] As can be seen from Table 1, for the artificial contaminated solution of green algae provided by Comparative Example 1, due to the absence of polyvinylpyrrolidone, it is difficult to simulate the formation of a green algal biofilm. However, for the artificial contaminated solution of green algae provided by Example 1, it can simulate the formation of a green algal biofilm on the surface of the insulator. The green algal biofilm is a complex microbial community formed by green algae and their secreted extracellular polymeric substances (EPS) on the insulator. The favorable microenvironment constructed by the green algal biofilm can promote nutrient cycling and physical protection, significantly promoting the growth of green algae itself. This makes the artificial contaminated solution of green algae provided by Example 1 have significantly improved green algal growth rate, biomass, and attachment after contamination on the surface of the insulator, being closer to the green algal attachment situation on the surface of the insulator in the natural environment.

[0065] Table 1: Growth and attachment of green algae on the surface of the insulator

[0066]

[0067] Experimental Example 2

[0068] In Experimental Example 2 of this application, the performance of the green algae artificial contamination solution in Example 1 was tested. For the performance test, the green algae artificial contamination solution provided in Example 1 and deionized water were diluted respectively according to the volume ratios of 1:2, 1:5, 1:10, and 1:20, and the pH value was adjusted to 7.5 using NaOH or HCl. Then, after contaminating the surface of the insulator with the green algae artificial contamination solution at different dilution multiples, the growth and attachment of green algae on the surface of the insulator were tested. The performance test results are shown in Table 2.

[0069] Table 2: Growth and attachment of green algae on the surface of insulators contaminated with green algae artificial contamination solution at different dilution multiples

[0070]

[0071] As can be seen from Table 2, for the green algae artificial contamination solution provided in Example 1 of this application, at different dilution multiples, the biomass of green algae increases per unit volume and adheres to the surface of the insulator, which can simulate the microbial contamination on the surface of the insulator.

[0072] The above embodiments are only used to illustrate the technical solutions of this application, rather than to limit them; although this application has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements for some or all of the technical features; and these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of this application.

Claims

1. An artificial polluted solution of green algae, characterized in that, Comprising: A green algae culture medium, a green algae concentrate, artificial fouling particles, and a non-ionic surfactant solution; The artificial fouling particles are diatomite / sodium chloride composite particles.

2. The artificial green algae contamination solution according to claim 1, characterized in that, In the green algae artificially contaminated solution, the volume mass ratio of the green algae culture medium, the green algae concentrate, the non-ionic surfactant solution, and the artificial fouling particles is 500~1500 mL: 50~150 mL: 50~150 mL: 5~15 g.

3. The artificial pollution solution of green algae according to claim 1, characterized in that, The mass ratio of diatomite to sodium chloride in the artificial fouling particles is 3~9:1; The concentration of the non-ionic surfactant solution is 2~10 mg / L.

4. A green algae artificial contamination solution according to claim 1, wherein, The green algae culture medium includes NaNO3, K2HPO4·3H2O, MgSO4·7H2O, CaCl2·2H2O, KH2PO4, NaCl, Na2CO3, and FeCl3·6H2O, Fe-EDTA solution, trace element solution, soil extract, jackfruit leaf extract, and cow ear maple leaf extract; The solutes in the trace element solution include H3BO3, ZnSO4·7H2O, MnCl2·4H2O, CuSO4·5H2O, and (NH4)6Mo7O2·4H2O.

5. A green algae artificial contamination solution according to claim 1, characterized in that, In 500~1500 mL of the green algae culture medium, it includes 0.1~0.5 g of NaNO3, 0.05~0.1 g of K2HPO4·3H2O, 0.05~0.1 g of MgSO4·7H2O, 0.01~0.05 g of CaCl2·2H2O, 0.1~0.25 g of KH2PO4, 0.01~0.05 g of NaCl, 1~3 g of Na2CO3, and 0.001~0.01 g of FeCl3·6H2O, 0.5~2 mL of Fe-EDTA solution with a concentration of 1~5 mmol, 0.5~2 mL of trace element solution, 20~60 mL of soil extract, 5~20 mL of jackfruit leaf extract, 5~20 mL of cow ear maple leaf extract; In 0.5~2 mL of the trace element solution, the solutes include 1~5 mg of H3BO3, 0.1~0.5 mg of ZnSO4·7H2O, 1~3 mg of MnCl2·4H2O, 0.05~0.1 mg of CuSO4·5H2O, and 0.02~0.06 mg of (NH4)6Mo7O2·4H2O.

6. A preparation method of an artificial pollution solution of green algae, characterized in that, For preparing the green algae artificially contaminated solution as described in any one of claims 1-5, it includes the following steps: Step S1: Mix the weighed NaNO3, K2HPO4·3H2O, MgSO4·7H2O, CaCl2·2H2O, KH2PO4, NaCl, Na2CO3, FeCl3·6H2O, Fe-EDTA solution, trace element solution, soil extract, jackfruit leaf extract, cow ear maple leaf extract, and distilled water evenly, and sterilize to obtain the green algae culture medium; Step S2: Inoculate the green algae concentrate into the green algae culture medium for cultivation to obtain a green algae suspension; Step S3: Add the artificial fouling particles to the green algae suspension for dispersion to obtain a green algae artificially fouled suspension; Step S4: Add the non-ionic surfactant solution to the artificial suspension of green algae soiling and mix evenly to obtain the artificially soiled green algae solution.

7. The preparation method of an artificial pollution solution of green algae according to claim 6, wherein In step S1, the preparation method of the Fe-EDTA solution includes: dissolving Na2EDTA in distilled water as solution A, dissolving FeCl3·6H2O in HCl solution as solution B, and mixing and stirring solutions A and B evenly to obtain the Fe-EDTA solution; The preparation method of the trace element solution includes: dissolving H3BO3, ZnSO4·7H2O, MnCl2·4H2O, CuSO4·5H2O, (NH4)6Mo7O2·4H2O in distilled water to obtain the trace element solution; The preparation method of the soil extract includes: adding distilled water and unfertilized soil to a container, sealing the mouth of the container with a breathable plug, heating with boiling water, cooling, precipitating, and filtering in sequence, and taking the supernatant of the filtered liquid and sterilizing it to obtain the soil extract; The preparation method of the jackfruit leaf extract includes: adding distilled water to fresh jackfruit leaves, heating to boiling, cooling, and filtering in sequence, and taking the supernatant of the filtered liquid and sterilizing it to obtain the jackfruit leaf extract; The preparation method of the earleaf maple leaf extract includes: adding distilled water to earleaf maple leaf powder, heating to boiling, cooling, and filtering in sequence, and taking the supernatant of the filtered liquid and sterilizing it to obtain the earleaf maple leaf extract.

8. A method for preparing an artificial polluted solution of green algae according to claim 6, characterized in that, In step S2, the light intensity for the cultivation is 4000 - 6000 lux; The light cycle for the cultivation is 12 hours of light followed by 12 hours of darkness in sequence; The temperature for the cultivation is 20 - 30 °C; The pH value for the cultivation is 6.5 - 8.

0.

9. The preparation method of an artificial pollution solution of green algae according to claim 6, characterized in that, In step S3, the preparation method of the artificial soiling particles includes: mixing diatomite particles, solid sodium chloride particles, and deionized water evenly, evaporating the water and then grinding to obtain the artificial soiling particles.

10. Application of an artificial pollution solution of green algae in the research and development of an efficient insulator cleaning technology, characterized in that, Use of the artificially soiled green algae solution according to any one of claims 1 - 5 in the research and development of high-efficiency insulator cleaning technology.

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