Fusarium solani and application thereof

Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to fusarium solani and application thereof. The fusarium solani Jing-YSD-PDA2 is separated and purified from fresh cyperus esculentus by adopting a tissue block separation method and a streak separation method, and the fusarium solani Jing-YSD-PDA2 has relatively good IAA (Indoleacetic Acid) production capacity and nitrogen fixation activity; in addition, the strain has a certain inhibition effect on mucor rouxii and water chestnut stem rot pathogenic bacteria curvularia lunata; the strain has a strong inhibition effect on the pathogenic bacterium fusarium oxysporum of the horseshoe fusarium wilt, the inhibition rate is 61.00%, the biocontrol potential is good, and a new strain resource can be provided for selection of biocontrol microbial agents for the horseshoe fusarium wilt and stem rot of the horseshoe fusarium wilt and the stem rot of the horseshoe fusarium wilt and the stem rot of the horseshoe fusarium wilt.

Description

Technical Field

[0001] The present invention belongs to the technical field of microbiology, and particularly relates to a Fusarium solani and its application. Background Art

[0002] Cyperus esculentus L., also known as Cyperus esculentus, iron water chestnut, etc., is a perennial herb of the genus Cyperus in the Cyperaceae family. It is native to the Mediterranean region, northern Africa, along the Nile River, etc. The main cultivation areas in China are concentrated in southern Xinjiang, Ordos in Inner Mongolia, Jilin and other places (Sun Rui, Zhong Peng, Liu Zedong, et al. Utilization value and industrial development status of Cyperus esculentus [J]. Feed Industry, 2023, 44(21): 19-24.). The protein content of Cyperus esculentus is similar to that of oats (10% - 13%), and the albumin content is as high as 80% - 95%; the oil of Cyperus esculentus is mainly oleic acid, and the oleic acid content can reach 65% - 75% (Duan Shuai, Zhang Dejian, Yao Yujun, et al. Research progress on the nutritional value and processing application of Cyperus esculentus [J]. Food Science and Technology, 2022(007): 047.); the fat content of Cyperus esculentus is 18.7% - 26.7%, the soluble sugar content is 15% - 25%, the dietary fiber content is 6.7% - 11.0%, and the starch content is 25% - 40%, among which the amylose content is 13.9% and the amylopectin content is 77.4% (Chen Lina, Shi Mao. Study on the determination of amylose and amylopectin content in Cyperus esculentus by dual-wavelength method [J]. Food Science and Technology, 2010(8): 3). It is a multi-purpose crop integrating grain, oil and feed, and can be widely used in the preparation of food, biodiesel, feed, etc. Cyperus esculentus grows rapidly, has well-developed roots, strong tillering and regeneration ability, and has few diseases during the whole growth period and does not require prevention and control; it has the characteristics of being drought-tolerant, waterlogging-tolerant, barren-tolerant, salt-alkali-tolerant, strong stress resistance and stress tolerance, and wide adaptability. It can be planted on low, medium and high fertility lands such as sandy soil, albic soil, saline-alkali land, black soil and wasteland slopes (Sun Rui, Zhong Peng, Liu Zedong, et al. Utilization value and industrial development status of Cyperus esculentus [J]. Feed Industry, 2023, 44(21): 19-24.). It is very suitable for planting on marginal lands such as desertified lands, and can contribute to the national edible oil and food security while not competing for land with staple grains. It is a high-quality crop that can be used for saline-alkali land improvement and marginal land utilization, and plays an important role in soil and water conservation, wind prevention and sand fixation, and protection of the natural ecological environment.

[0003] At present, there is little research on the endophytic fungi in the hardy Cyperus oleifera with existing technologies, especially the research on the endophytic fungi of Cyperus oleifera in plant growth promotion and biological control. Oyedara et al. (Oyedara OO, Rufai AB, Okunlola GO, et al. Nutritional and Endophytic Composition of Edible Tubers of Tiger nut (Cyperus esculentus L.) [J]. Jordan Journal of Biological Sciences, 2022, 15 (4). DOI: 10.54319 / jjbs / 150408.) isolated two endophytic fungi from Cyperus esculentus, namely Saccharomyces cerevisiae and Candida tropicalis; Wang Saisai et al. (Wang Saisai. Study on the growth-promoting effect of endophytes of Cyperus esculentus and the synthesis mechanism of flavonoids [D]. Jiangnan University, 2022.) studied the community diversity of different varieties of Cyperus esculentus (Cyperus esculentus I and Cyperus esculentus II).

[0004] In summary, it is necessary to conduct in-depth research on the role of endophytic fungi in plant growth promotion and biological control in order to provide strain resources for the research of microbial agents in biological control and provide a theoretical basis and scientific guidance for biological control in agricultural production.

[0005] Eleocharis dulcis, also known as water chestnut, belongs to the same family as Cyperus oleiferus, and both belong to the Cyperaceae family. Eleocharis dulcis belongs to the genus Eleocharis in the Cyperaceae family. During its growth, it often encounters disease problems, such as Eleocharis wilt and stem rot. At present, the traditional control methods for Eleocharis wilt and stem rot mainly include agricultural control and chemical control. In recent years, the traditional agricultural control cannot fundamentally control the occurrence of diseases, and the control effect is unstable; the long-term use of chemical pesticides will cause pathogens to develop drug resistance, and excessive use of pesticides will also damage the soil, aggravate the pollution of pesticides to the environment, and cause too many toxic and harmful substances to remain in crops. Therefore, biological control methods are increasingly valued by people. Plant endophytes are a natural resource fungus for biological control of plant diseases. Most plants are parasitic on a large number of endophytic fungi, which have antibacterial activity and can be used to defend against pathogen attacks.

[0006] Horseshoe and Cyperus rotundus belong to the Cyperaceae family and have certain similarities in plant morphology, physiological characteristics, and growth habits. This means that they may have similar susceptibility to certain pathogens or similar defense mechanisms when facing diseases. Therefore, the endophytic fungi isolated from Cyperus rotundus are expected to be used for the prevention and treatment of horseshoe stem rot. Summary of the invention

[0007] In order to overcome the deficiencies and shortcomings of the prior art, the primary purpose of the present invention is to provide a Fusarium solani.

[0008] Another object of the present invention is to provide the application of the above-mentioned Fusarium solani.

[0009] Another object of the present invention is to provide a microbial fertilizer.

[0010] The fourth object of the present invention is to provide an antibacterial agent.

[0011] The purpose of the present invention is achieved through the following technical solutions:

[0012] A Fusarium solani, named Fusarium solani Jing-YSD-PDA2, was deposited in Guangdong Microbiological Culture Collection Center on July 11, 2024, with the deposit address being: 5th Floor, Building 59, No. 100, Xianlie Middle Road, Guangzhou, and the deposit number is: GDMCC No: 64860;

[0013] The Fusarium solani is isolated from Cyperus oleifera and is an endophytic fungus;

[0014] The Fusarium solani has the following morphological characteristics:

[0015] On PDA medium, the colonies of strain Jing-YSD-PDA2 were white, nearly round, with fuzzy hyphae and septa inside the hyphae that separated the hyphae into several segments.

[0016] Application of the Fusarium solani and / or its fermentation liquid in the field of plant growth promotion or soil remediation;

[0017] Application of the Fusarium solani and / or its fermentation liquid in the preparation of indoleacetic acid;

[0018] Application of the Fusarium solani and / or its fermentation liquid in the field of preparing microbial fertilizer;

[0019] The use of the Fusarium solani and / or its fermentation liquid in inhibiting plant pathogens, in preventing and controlling plant diseases caused by plant pathogens, or in the preparation of plant pathogen inhibitors or biocontrol agents;

[0020] The plant pathogen is at least one of Mucor rouxianus, Curvularialunata and Fusarium commune;

[0021] A plant growth promoter, comprising the above-mentioned Fusarium solani and / or its fermentation liquid;

[0022] A soil conditioner, comprising the above-mentioned Fusarium solani and / or its fermentation broth;

[0023] A microbial fertilizer, comprising the above-mentioned Fusarium solani and / or its fermentation broth;

[0024] An antibacterial agent, comprising the above-mentioned Fusarium solani and / or its fermentation broth;

[0025] The present invention has the following advantages and effects compared with the prior art:

[0026] (1) An endophytic fungus Jing-YSD-PDA2 was isolated and purified from Cyperus esculentus L. by tissue block separation method and streak plate method. Through morphological and molecular identification, the endophytic fungus of Cyperus esculentus L. was identified as Fusarium solani.

[0027] (2) The Fusarium solani Jing-YSD-PDA2 provided by the present invention has good IAA-producing ability, and the mass concentration of IAA produced is 0.52 mg / L.

[0028] (2) The Fusarium solani Jing-YSD-PDA2 provided by the present invention was inoculated on Ashby's medium and continuously cultured for three generations, and it could still grow normally and had a good growth state, indicating that it has nitrogen fixation activity.

[0029] (3) The Fusarium solani Jing-YSD-PDA2 provided by the present invention has a certain inhibitory effect on Mucor rouxii and Curvularia lunata, the pathogen of stem rot of Eleocharis tuberosa; it has strong resistance to Fusarium oxysporum, the pathogen of wilt of Eleocharis tuberosa, and the inhibition rate is 61.00%. It has good biocontrol potential and can provide new strain resources for the selection of biocontrol agents for wilt and stem rot of Eleocharis tuberosa.

[0030] (4) The Fusarium solani Jing-YSD-PDA2 provided by the present invention has the ability to produce indoleacetic acid (IAA) and nitrogen fixation ability at the same time, which can help plants fully absorb and utilize the required nutrients, promote plant growth, and has the potential to be developed into a microbial fertilizer. Description of the Drawings

[0031] Figure 1 is the colony morphology diagram of the strain Jing-YSD-PDA2.

[0032] Figure 2 is the morphological diagram of the strain Jing-YSD-PDA2 under the microscope.

[0033] Figure 3It is the gel electrophoresis result diagram of the 18S rDNA PCR amplification product of strain Jing-YSD-PDA2.

[0034] Figure 4 It is the phylogenetic tree result diagram constructed based on the 18S rDNA sequence of strain Jing-YSD-PDA2.

[0035] Figure 5 It is the qualitative analysis result diagram of the IAA-producing ability of strain Jing-YSD-PDA2.

[0036] Figure 6 It is the PC standard curve (low-concentration standard curve) and S2 standard curve (high-concentration standard curve) diagram.

[0037] Figure 7 It is the result display diagram of the nitrogen fixation ability of strain Jing-YSD-PDA2 in Ashby's medium (the 3rd generation).

[0038] Figure 8 It is the antagonistic effect display diagram of strain Jing-YSD-PDA2 against Mucor rouxianus. Among them, A is the control; B: treatment (Mucor rouxianus on the left, Jing-YSD-PDA2 on the right).

[0039] Figure 9 It is the antagonistic effect display diagram of strain Jing-YSD-PDA2 against the pathogen of water chestnut stem rot (Curvularia lunata). Among them, A is the control; B is the treatment (strain Jing-YSD-PDA2 on the left, the pathogen of fusarium wilt on the right).

[0040] Figure 10 It is the antagonistic effect display diagram of strain Jing-YSD-PDA2 against the pathogen of water chestnut fusarium wilt (Fusarium oxysporum). Among them, A is the control; B is the treatment (strain Jing-YSD-PDA2 on the left, the pathogen of fusarium wilt on the right). Specific implementation manners

[0041] The present invention will be further described in detail below in conjunction with the embodiments and the accompanying drawings, but the implementation manners of the present invention are not limited thereto.

[0042] 1. Materials and reagents involved in the embodiments

[0043] (1) Test pathogens: Curvularia lunata, strain number: 142975, provided by Beina Chuanglian Biotechnology Co., Ltd.; Mucor rouxianus, strain number: AS3.3447, provided by Shanghai Biological Preservation Technology Center; Fusarium commune provided by China Center for Type Culture Collection, strain preservation number: CCTCC AF 2013028.

[0044] (2) Cyperus esculentus tubers: Cyperus esculentus tubers were collected from 20 healthy and disease-free individual Cyperus esculentus plants with strong growth in Xinshao Town, Shaoguan City, Guangdong Province (N24°46’, E113°39’, altitude 105 m).

[0045] (3) Test medium: Potato Dextrose Agar (PDA) medium: 5.0 g of potato leaching powder, 20.0 g of glucose, 15.0 g of agar, 1 L of distilled water. Heat and boil until completely dissolved, autoclave at 121 °C for 15 min, cool to about 55 °C, add two antibiotics, kanamycin base and ampicillin, mix well and pour into plates for standby, which is used for the cultivation of plant pathogenic fungi and the determination of the antagonistic effect of strains.

[0046] Table 1 List of main reagents

[0047]

[0048] 2. Data statistics and processing in the examples

[0049] Each experiment was conducted with 3 parallel tests. Excel was used for data sorting and analysis, and MEGA 11.0 software was used for graphing.

[0050] Example 1 Isolation and purification of strains

[0051] 1. Experimental method

[0052] 1.1 Sample treatment

[0053] (1) Sample pretreatment: The collected Cyperus esculentus tuber samples were placed in a labeled self-sealing bag and stored at 4 °C, and then taken back to the laboratory. Rinse the surface with tap water to remove sediment and wash with tap water for 5 min.

[0054] (2) Surface disinfection and detection of strains: Refer to the method of Qu Siqi (Qu Siqi. Research on the effect of endophyte fermentation on the ginsenoside content of Oplopanax elatus Nakai [D]. Yanbian University, 2020.). Surface disinfection treatment: Immerse in 75% (v / v) ethanol solution for 2 min, rinse with sterile water 5 times, immerse in 2% (w / w) NaClO solution for 30 s, rinse with sterile water 5 times, repeat 3 - 4 times, and blot dry with sterile filter paper. Take 0.1 mL of the sterile water from the last rinse and spread it on PDA medium, incubate in an inverted position at 28 °C for 5 - 7 d to detect the surface sterilization effect, ensure complete surface disinfection, and the isolated strain is an endophytic fungus rather than an exogenous contaminant.

[0055] 1.2 Isolation and purification of endophytic fungi

[0056] The method described by Zang Sanli (Zang Sanli. Isolation, Screening and Characterization of Endophytic Fungi from Plants Producing Limonin [D]. Taiyuan: Shanxi University, 2019.) was followed. The surface-sterilized samples were cut into 1 cm × 1 cm pieces with a sterile blade under sterile conditions and inoculated onto PDA solid medium plates. Each sample was replicated on 3 plates and incubated in an inverted position at 28 °C. After the growth of the mycelium around the sample, a small amount of mycelium was picked from the edge with an inoculation loop and transferred to a new PDA medium. After the mycelium grew into colonies, the outer-edge mycelium was picked with an inoculation loop and repeatedly cultured until a single strain was obtained. The strain was transferred to a slant medium and stored at 4 °C. To inhibit bacterial growth, 20 μg / mL kanamycin and 20 μg / mL ampicillin were added to the PDA medium.

[0057] 1.3 Identification of endophytic fungi

[0058] (1) Morphological identification of endophytic fungi:

[0059] The strains isolated and purified from Cyperus esculentus tubers were inoculated onto PDA plate medium and cultured at 28 °C. The characteristics of the colonies were observed. The mycelium (including sporophores) of the purified strains was made into slides, and the morphological characteristics of the mycelium, spore-bearing structures, and spores were observed under a microscope. The fungal classification handbook was consulted for the preliminary identification of the strains. Combining the cultural characteristics, the strains were identified to the genus level, and then the species were identified according to the classification criteria of different genera.

[0060] (2) Identification of the ITS gene sequence of the strains:

[0061] Genomic DNA of the isolated strains was extracted according to the instructions of the Ezup Column Fungal Genomic DNA Extraction Kit. Using this DNA as a template, PCR amplification was performed with ITS1 (5’-TCCGTAGGTGAACCTGCGG-3’) and ITS4-R (5’-TCCTCCGCTTATTGATATGC-3’) as primers. The PCR amplification system was 25.0 μL, including 1.0 μL of template DNA, 12.5 μL of 50 mM MgSO4, 1 μL each of 10 μmol / L primers ITS1 and ITS4, and 9.5 μL of ddH2O. The PCR reaction conditions were: pre-denaturation at 95 °C for 5 min; denaturation at 94 °C for 30 s, annealing at 57 °C for 30 s, extension at 72 °C for 90 s; the cycle of denaturation to extension was repeated 30 times; final extension at 72 °C for 10 min; and finally cooled to 4 °C for storage. After detecting the PCR products by agarose gel electrophoresis, they were sent to Shanghai Sangon Biotech Co., Ltd. for sequencing. The obtained sequences were submitted to the NCBI database GenBank for BLAST homology alignment analysis, and a phylogenetic tree was constructed by the neighbor-joining method using MEGA11.0 to determine the taxonomic status of the strains.

[0062] Molecular weight comparison: The electrophoresis bands of the sample were compared with those of the marker to determine the DNA molecular weight of the sample strain.

[0063] 2. Result analysis

[0064] 2.1 Isolation and identification of endophytic fungi

[0065] For the 1-year-old Cyperus esculentus tubers, potato dextrose agar medium (PDA) was used for isolation and purification, and 1 strain of endophytic fungi was obtained. This strain of endophytic fungi was numbered: Jing-YSD-PDA2.

[0066] 2.2 Morphological identification

[0067] The colony morphology of strain Jing-YSD-PDA2 was observed, and its mycelium and spore morphology were observed by optical microscope. The results are shown in Figure 1 and Figure 2 . Among them, on the PDA medium, the colony of strain Jing-YSD-PDA2 was white, nearly circular, with villous mycelium, and there were transverse septa in the mycelium to divide the mycelium into several segments.

[0068] 2.3 Molecular biology identification

[0069] Strain Jing-YSD-PDA2 was amplified using universal fungal primers. After detecting the PCR products by agarose gel electrophoresis, a 1323bp fragment ( Figure 3 ) was obtained. The PCR products were sequenced, and the sequencing results are as follows:

[0070] Strain Jing-YSD-PDA2 (1323bp):

[0071]

[0072] The above sequences were submitted to the NCBI database GenBank for BLAST homology analysis. After similar sequences were downloaded, the phylogenetic tree was constructed using the neighbor-joining method using MEGA 11.0 software. The results are shown in Figure 4 Strain Jing-YSD-PDA2, Fusarium solani (ON950402.1) and Fusarium solani (ON026100.1) were clustered on one branch, and the similarity between strain Jing-YSD-PDA2 and Fusarium solani was 99.62%, indicating a close relationship.

[0073] Combined with morphological characteristics, the strain Jing-YSD-PDA2 was identified as Fusarium solani, and named Fusarium solani Jing-YSD-PDA2. It was deposited in Guangdong Provincial Microbiological Culture Collection on July 11, 2024. The storage address is: 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou, and the storage number is: GDMCCNo: 64860.

[0074] Example 2 Determination of IAA production and nitrogen fixation ability of strains

[0075] 1. Experimental Methods

[0076] 1.1 Determination of IAA production capacity

[0077] (1) Preparation of PC colorimetric solution: weigh 12 g of FeCl3 and dissolve it in 300 mL of distilled water. Then slowly add 429.7 mL of 98% H2SO4. After cooling, dilute to 1 L. The measurement range is 0.3-20 mg / L.

[0078] Preparation of S2 colorimetric solution: Dissolve 4.5g FeCl3 in 300mL distilled water, then slowly add 587.4mL H2SO4 with a mass fraction of 98%, and adjust the volume to 1L after cooling. The IAA range of the measurement is 5-200mg / L.

[0079] (2) Qualitative analysis: The Salkowski method (Arun K. Screening and identification of abiotic stress-responsive efficient antifungal Pseudomonas spp. from ricerhizospheric soil. [J].

[0080] (Biotechnologia, 2021, 102(1): 15 - 19.) It was inoculated into King's liquid medium, with 3 replicates, cultured with shaking at 28 °C and 120 r / min for 5 days, centrifuged at 8000 r / min for 10 min, and the supernatant was taken into a test tube. 2 mL of PC colorimetric solution and S2 colorimetric solution were added respectively, and it was left to stand in the dark at room temperature for 30 min to observe the color change. If the color turns red, it indicates the production of IAA, and the darker the color, the more IAA is produced. If there is no color change, no IAA is produced.

[0081] (3) Quantitative analysis: Standard curves of two groups of IAA were drawn using standards for quantitative testing. The concentration gradients of the first group were 2.5, 5.0, 7.5, 10.0, 12.5, 15.0 mg / L, and those of the second group were 25, 50, 75, 100, 125, 150 mg / L. According to the above color development method, the absorbance was measured at 530 nm using an ultraviolet spectrophotometer to draw the standard curve, calculate the standard curve equation. The color-developed strain solution in (2) was placed at 530 nm of the ultraviolet spectrophotometer to measure the OD value. Using the distilled water added with PC colorimetric solution and S2 colorimetric solution respectively as the control, the absorbance values OD of the bacterial solutions of each strain were measured at 530 nm. 530 The amount of IAA in the culture solution per unit volume was calculated according to the standard curve.

[0082] 1.2 Determination of nitrogen fixation ability

[0083] The nitrogen fixation ability was detected by nitrogen-free medium (Cao Ziqin, Zhao Xiaoqing, Zhang Xiangqian, et al. Effects of nitrogen application level on nitrogen, phosphorus, potassium accumulation, distribution and yield of Cyperus esculentus L. in sandy soil [J / OL]. Acta Agronomica Sinica, 1 - 14 [2024 - 04 - 22]). The activated strain to be tested was inoculated on Ashby medium and cultured in a constant temperature incubator at 28 °C for 7 days, and continuously subcultured 3 times. If the strain grows normally on Ashby medium, it indicates that the strain has nitrogen fixation activity.

[0084] 2. Result analysis

[0085] 2.1 Results of IAA production ability determination

[0086] The IAA production ability of the endophytic fungal strain Jing - YSD - PDA2 isolated from Cyperus esculentus L. was determined. Through qualitative analysis by the Salkowski method, the color of the test tube of the strain Jing - YSD - PDA2 became light pink, indicating that this endophytic fungus has good IAA production ability ( Figure 5 ).

[0087] After the determination of the IAA standard product, the absorbance value was measured at a wavelength of 530 nm using an enzyme-labeled instrument, and the obtained data was used to plot the standard curve equation. Among them, the PC standard curve equation was y = 0.0312x + 0.0145 and the S2 standard curve equation was y = 0.0094x + 0.2171( Figure 6 ), and the R 2 values were 0.9931 and 0.9424 respectively, indicating a good linear relationship. By measuring the OD 530 value, plotting the standard curve equation, measuring the absorbance value of the sample to be tested at a wavelength of 530 nm using an enzyme-labeled instrument, and calculating the amount of IAA produced by the strain according to the standard curve equation, the concentration of IAA produced by the strain Jing-YSD-PDA2 was calculated to be 0.52 mg / L.

[0088] Table 2 Qualitative and Quantitative Screening Results of IAA Secretion by Strain Jing-YSD-PDA2

[0089]

[0090] 2.2 Determination Results of the Nitrogen-Fixing Ability of Strain Jing-YSD-PDA2

[0091] The strain Jing-YSD-PDA2 was inoculated on Ashby's medium for cultivation. For the first generation, the mycelium was picked with an inoculation loop for cultivation on a new plate. The above method was repeated for the second generation, and after continuous cultivation for three generations( Figure 7 ), it was found that Jing-YSD-PDA2 could grow normally and had a good growth state, indicating its nitrogen-fixing activity.

[0092] Example 3

[0093] 1. Experimental Method

[0094] The endophytic fungus Jing-YSD-PDA2 isolated and purified from Example 1 was screened by the plate confrontation method (Cui Y, Zhu Y, Dong G, et al. Evaluation of the control efficacy of antagonistic bacteria from V-Ti magnetite mine tailings on kiwifruit brown spots in pot and field experiments[J]. Frontiers in Microbiology, 2024, 15:1280333-1280333.). First, the endophytic fungus Jing-YSD-PDA2 of Cyperus esculentus and the pathogenic bacteria (Curvularia lunata, strain number: 142975, Mucor rouxianus, strain number: AS3.3447; Fusarium commune, preservation number: CCTCC AF 2013028) were inoculated on two PDA media for cultivation. After suitable growth, a fungal plug was punched out using a borer The two fungal plugs were inoculated on the same PDA medium, with a distance of about 4 cm between the two fungal plugs. Each treatment was repeated 3 times. The fungal plug inoculated with the pathogenic bacteria alone was used as the blank control. It was placed in a constant temperature incubator and incubated at 28 °C for about 3-5 days. The colony diameters of the endophytic fungus, the pathogenic bacteria, and the pathogenic bacteria used as the blank control were measured, and the inhibition rate was calculated:

[0095]

[0096] 2. Result analysis

[0097] The antagonistic effects of the strain Jing-YSD-PDA2 against Mucor rouxianus, the pathogen of Cyperus rot (Curvularia lunata), and the pathogen of Cyperus wilt (Fusarium commune) were observed by the plate confrontation method. After 5 days of confrontation culture, the colony diameters of the control pathogenic bacteria and the colony diameters of the pathogenic bacteria pointing to the fungus in the treatment were measured, and the inhibition rate was calculated. The strain Jing-YSD-PDA2 had certain inhibitory effects on Mucor rouxianus and the pathogen Curvularia lunata of Cyperus rot (Table 3, Figures 8 - 9 ), and had a good inhibitory effect on the pathogen of Cyperus wilt. Among them, the inhibition rate of the strain Jing-YSD-PDA2 against Fusarium commune was 61.00% (Table 3, Figure 10 ).

[0098] Table 3 Inhibition rates of the strain Jing-YSD-PDA2 against different pathogenic bacteria

[0099]

[0100] Note: "+++" represents strong, "++" represents medium, "+" represents weak, and "—" represents no antagonistic effect

[0101] The above embodiments are preferred embodiments of the present invention. However, the embodiments of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications made without departing from the spirit and principle of the present invention shall be equivalent replacement methods and are all included in the protection scope of the present invention.

Claims

1. A Fusarium solani, characterized in that The name is Fusarium solani Jing-YSD-PDA2, and it was deposited in the Guangdong Provincial Microbiological Culture Collection Center on July 11, 2024. The storage address is: 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou City, and the storage number is: GDMCC No: 64860.

2. Use of the Fusarium solani and / or its fermentation liquid according to claim 1 in the field of plant growth promotion or soil remediation.

3. Use of the Fusarium solani according to claim 1 and / or its fermentation liquid in the preparation of indoleacetic acid.

4. Use of the Fusarium solani described in claim 1 and / or its fermentation liquid in the field of preparing microbial fertilizer.

5. Use of the Fusarium solani and / or its fermentation liquid according to claim 1 in inhibiting plant pathogens, in preventing and controlling plant diseases caused by plant pathogens, or in the preparation of plant pathogen inhibitors or biocontrol agents.

6. The use according to claim 5, characterized in that: The plant pathogenic bacteria is at least one of Mucor rouxianus, Curvularia lunata and Fusarium commune.

7. A plant growth promoter, characterized in that The method comprises the Fusarium solani according to claim 1 and / or its fermentation liquid.

8. A soil remediation agent, characterized in that The method comprises the Fusarium solani according to claim 1 and / or its fermentation liquid.

9. A microbial fertilizer, characterized in that The method comprises the Fusarium solani according to claim 1 and / or its fermentation liquid.

10. An antibacterial agent, characterized in that The method comprises the Fusarium solani according to claim 1 and / or its fermentation liquid.

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