Fermentation probiotic agent as well as preparation method and application thereof

Abstract

The invention relates to a fermentation probiotic agent and a preparation method and application thereof.The preparation method of the fermentation probiotic agent comprises the steps that raw materials including, by weight, 2-4 parts of composite probiotic powder, 10-15 parts of fructus psoraleae, 8-13 parts of radix morindae officinalis, 35-42 parts of straw, 5-10 parts of soybean meal, 6-8 parts of trehalose and 18-24 parts of deionized water are weighed; fully mixing the compound probiotic powder, trehalose and deionized water, and performing anaerobic closed culture to obtain activated bacterial liquid for later use; respectively drying the fructus psoraleae, the radix morindae officinalis, the straw and the soybean meal, crushing, sieving with a 100-mesh sieve, uniformly mixing, adding into the activated bacterial liquid, carrying out anaerobic sealed fermentation, and then carrying out vacuum freeze drying to obtain the fermented probiotic agent. The fermented probiotics are used as the feed additive to be added into the basic ration, so that the feed additive has the effects of promoting the growth of livestock animals and improving the feed utilization rate, is remarkable in effect, and also has the effect of improving the immune function of the cattle.

Description

Technical Field

[0001] The invention belongs to the technical field of feed additives, and specifically relates to a fermented probiotic agent and a preparation method and application thereof. Background Art

[0002] Antibiotics are used in animal feed as feed additives to promote animal growth and prevent certain diseases, and have made great contributions to the development of the feed industry and animal husbandry. However, long-term use of antibiotics can cause bacteria to develop resistance to antibiotics, and ultimately cause antibiotics to lose their ability to inhibit or kill bacteria. Antibiotics cannot be effectively degraded in the animal's body. Long-term use will result in a large amount of antibiotics remaining in the animal's body. These antibiotics will be excreted from the animal's excrement and enter the environment, causing pollution to the ecological environment. These antibiotic residues may also enter the human body through the food chain, causing harm to human health, such as damage to the liver, kidneys and other organs.

[0003] Therefore, seeking new alternatives to antibiotics has become an urgent problem to be solved. Probiotics are generally considered safe as feed additives. Therefore, studying more fermented probiotics that can promote the growth of livestock and improve their immune function can accelerate the research process of replacing antibiotics with natural, environmentally friendly and green feed additives, which has important practical significance. Summary of the invention

[0004] The purpose of the present invention is to solve the above problems and provide a fermented probiotic agent and a preparation method and application thereof.

[0005] The present invention achieves the above-mentioned purpose through the following technical solutions:

[0006] The present invention provides a method for preparing a fermented probiotic agent, and the specific steps are as follows:

[0007] 1. Weigh each raw material by weight, including 2-4 parts of compound probiotic powder, 10-15 parts of psoralea corylifolia, 8-13 parts of Morinda officinalis, 35-42 parts of straw, 5-10 parts of soybean meal, 6-8 parts of trehalose, and 18-24 parts of deionized water;

[0008] 2. Fully mix the composite probiotic powder, trehalose and deionized water, perform anaerobic closed culture to obtain activated bacterial solution for later use;

[0009] 3. Dry and grind Psoralea corylifolia, Morinda officinalis, straw and soybean meal respectively, mix them evenly after passing through a 100-mesh sieve, and add them to the activated bacterial solution. After anaerobic sealed fermentation, vacuum freeze-drying is performed to obtain the fermented probiotic agent.

[0010] As a further optimization scheme of the present invention, in Steps 1 and 2, the compound probiotic powder comprises Bacillus licheniformis, Lactobacillus plantarum, and Lactobacillus acidophilus NCFM with a mass ratio of 1:1:1;

[0011] The Bacillus licheniformis has a product number of RC-JZ8322, and the Lactobacillus plantarum has a product number of XY-WSW-2325;

[0012] The viable counts of both the Bacillus licheniformis and Lactobacillus plantarum are 6.2 - 6.8×10 7 cfu / g; the viable count of the Lactobacillus acidophilus NCFM is 7.3 - 7.8×10 8 cfu / g.

[0013] As a further optimization scheme of the present invention, the straw includes at least one of wheat straw, rapeseed straw, and peanut straw.

[0014] As a further optimization scheme of the present invention, in Step 2, the anaerobic sealed culture temperature is 32°C - 38°C and the time is 20 - 28 h.

[0015] As a further optimization scheme of the present invention, in Step 3, the anaerobic sealed fermentation temperature is 30°C - 40°C, the fermentation humidity is 30 - 40%, and the fermentation time is 5 - 7 days.

[0016] The present invention also provides a fermented probiotic agent prepared by the above preparation method.

[0017] The present invention also provides an application of the fermented probiotic agent in promoting the growth of livestock animals and improving the immune function of livestock animals.

[0018] As a further optimization scheme of the present invention, when the fermented probiotic agent is added to the feed as a feed additive, it can promote animal weight gain, improve the feed utilization rate of animals, and also increase the contents of immunoglobulin A and immunoglobulin G in the animal serum.

[0019] As a further optimization scheme of the present invention, the addition amount of the fermented probiotic agent in the feed is ≥6 wt%.

[0020] The beneficial effects of the present invention are as follows:

[0021] 1) The present invention uses the fermented probiotic as a feed additive and adds it to the basal diet. It has the effects of promoting the growth of livestock animals, improving the feed utilization rate, and the effect is significant. At the same time, it can also increase the contents of immunoglobulin A (IgA) and immunoglobulin G (IgG) in the serum of yellow cattle, indicating that the fermented probiotic of the present invention also has the function of improving the immune function of yellow cattle;

[0022] 2) In the present invention, wheat straw and peanut straw are used as fermentation substrates, and Chinese herbs Psoralea corylifolia and Morinda officinalis are added. The fermented probiotic agent is prepared by fermentation with a compound probiotic powder (Bacillus licheniformis RC-JZ8322, Lactobacillus plantarum XY-WSW-2325, Lactobacillus acidophilus NCFM with a mass ratio of 1:1:1). It has remarkable effects in promoting the growth of livestock and poultry, improving the feed utilization rate, and enhancing the immune function of livestock and poultry. It is speculated that there is a synergistic effect among the compound probiotic powder, wheat straw, peanut straw, and Chinese herbs Psoralea corylifolia and Morinda officinalis. Detailed implementation manners

[0023] The following further describes the present application in detail. It is necessary to point out here that the following specific implementation manners are only used to further illustrate the present application and cannot be construed as limiting the protection scope of the present application. Those skilled in the art can make some non-essential improvements and adjustments to the present application according to the above application content.

[0024] I. Materials

[0025] 1. Bacillus licheniformis, strain number RC-JZ8322, specification: freeze-dried powder, purchased from Tianjin Ruichuang Biotechnology Co., Ltd., product number RC-JZ8322;

[0026] 2. Bacillus licheniformis, strain number XHL2309, specification: freeze-dried powder, purchased from Hubei Xinhongli Chemical Industry Co., Ltd., product number XHL2309;

[0027] 3. Lactobacillus plantarum, strain number XY-WSW-2325, specification: freeze-dried powder, purchased from Shanghai Xuanya Biotechnology Co., Ltd., product number XY-WSW-2325;

[0028] 4. Lactobacillus plantarum, strain number BN1395, specification: freeze-dried powder, purchased from Shanghai Sentai Biotechnology Co., Ltd., product number BN1395;

[0029] 5. Lactobacillus acidophilus NCFM, strain number: SHBCCD24720, specification: freeze-dried powder, purchased from Shanghai Ruichu Biotechnology Co., Ltd., product number: SHBCC D24720;

[0030] 6. Psoralea corilyfolia L. is an important and commonly used traditional Chinese medicine, belonging to the Rosales order, the genus Psoralea of the Leguminosae family. It contains more than 30 chemical components such as coumarins, flavonoids, monoterpene phenols, benzofurans, etc., and has effects such as antibacterial, anti-tumor, antiviral, analgesic, anti-osteoporosis, and immune regulation. It was purchased from the First Affiliated Hospital of Anhui University of Chinese Medicine (Anhui Provincial Hospital of Traditional Chinese Medicine) located at No. 117, Meishan Road, Hefei City, Anhui Province;

[0031] 7. Morinda officinalis How, the dried root of the plant Morinda officinalis How of the genus Morinda of the Rubiaceae family, has various pharmacological effects such as tonifying the kidney yang, strengthening the bones and muscles, and anti-depression. It was purchased from the First Affiliated Hospital of Anhui University of Chinese Medicine (Anhui Provincial Hospital of Traditional Chinese Medicine) located at No. 117, Meishan Road, Hefei City, Anhui Province;

[0032] 8. The MRS liquid medium, MRS solid medium, and MRS plate medium are as follows:

[0033] MRS liquid medium: Peptone 20 g / L, yeast powder 5 g / L, beef powder 20 g / L, glucose 8 g / L, sodium chloride 5 g / L, dipotassium hydrogen phosphate 2 g / L, diammonium citrate 1 g / L, Tween 80 2 g / L. It is fully dissolved with water as the solvent and made up to 1 L in volume. The pH is adjusted to 6.5 - 7, and it is sterilized at 125 °C and 0.1 MPa for 20 min. The sterilized medium is poured into petri dishes and left to cool for later use;

[0034] MRS solid medium: On the basis of the MRS liquid medium, 1% (w / v) agar powder is added;

[0035] MRS plate medium: On the basis of the MRS liquid medium, 1.5% (w / v) agar powder is added.

[0036] The methods used in the present invention are all conventional methods known to those skilled in the art without special instructions. The raw materials, reagents and other materials used, without special instructions, are all commercially available products.

[0037] II. Methods

[0038] 2.1. Preparation of bacterial powder

[0039] The preserved / purchased Bacillus licheniformis RC-JZ8322, Bacillus licheniformis XHL2309, Lactobacillus plantarum XY-WSW-2325, Lactobacillus plantarum BN1395, and Lactobacillus acidophilus NCFM were respectively activated on MRS plate culture medium, and a single colony was selected and transferred to 100 mL of MRS liquid culture medium, and cultured at a constant temperature of 35°C±2°C for 12 h to obtain a seed solution, which was then inoculated into an MRS solid culture medium at an inoculum size of 1% (v / v), and then cultured at 35°C±2°C for 28 h to obtain a bacterial solution; after centrifuging the bacterial solution, the bacterial bodies were collected, washed with sterile physiological saline, and resuspended in 15% (w / w) reconstituted skim milk to obtain a bacterial suspension, and the bacterial suspension was freeze-dried to obtain bacterial powder of the corresponding bacteria for later use.

[0040] The bacterial powders of the corresponding bacteria obtained above were applied to the following experiments, wherein the number of viable bacteria of Bacillus licheniformis RC-JZ8322, Bacillus licheniformis XHL2309, Lactobacillus plantarum XY-WSW-2325, and Lactobacillus plantarum BN1395 were all 6.2-6.8×10 7 cfu / g; the number of viable bacteria of Lactobacillus acidophilus NCFM is 7.3-7.8×10 8 cfu / g.

[0041] 2.2 Preparation of fermented probiotics

[0042] The preparation method of the fermented probiotic agent comprises the following specific steps:

[0043] 1. Weigh each raw material by weight, including 2-5 parts of compound probiotic powder, 10-15 parts of psoralea corylifolia, 8-13 parts of Morinda officinalis, 35-42 parts of straw, 5-10 parts of soybean meal, 6-8 parts of trehalose, and 18-24 parts of deionized water;

[0044] The composite probiotic powder includes Bacillus licheniformis RC-JZ8322, Lactobacillus plantarum XY-WSW-2325, and Lactobacillus acidophilus NCFM in a mass ratio of 1:1:1;

[0045] 2. Fully mix the composite probiotic powder, trehalose and deionized water, and perform anaerobic closed culture (anaerobic closed culture temperature is 32°C to 38°C, time is 20 to 28h, preferably 34°C, 26h) to obtain activated bacterial solution for standby use;

[0046] 3. Dry and grind Psoralea corylifolia, Morinda officinalis, straw and soybean meal respectively, mix them after passing through a 100-mesh sieve, add them to the activated bacterial solution, and perform anaerobically sealed fermentation (the anaerobic sealed fermentation temperature is 30°C to 40°C, the fermentation humidity is 30-40%, the fermentation time is 5-7 days, preferably fermentation at 35°C and 37% fermentation humidity for 6 days), and then perform vacuum freeze-drying to obtain the fermented probiotic agent.

[0047] 2.3. To study the effects of different ratios of the preparation raw materials of the fermented probiotic agent on animal growth (weight gain), feed utilization rate, and immune function, experimental groups 1-9 and control groups 1-10 were set up as follows:

[0048] It should be noted that the only difference in the preparation raw materials of the fermented probiotic agent in experimental groups 1-7 lies in the different straws used, as shown in Table 1:

[0049] Table 1 Preparation raw materials of the fermented probiotic agent for experimental groups 1-12

[0050]

[0051] Note: The mass ratio of wheat straw to rapeseed straw is 1:1; the mass ratio of wheat straw to peanut straw is 1:1; the mass ratio of rapeseed straw to peanut straw is 1:1; the mass ratio of wheat straw, rapeseed straw, and peanut straw is 1:1:1.

[0052] Experimental group 8

[0053] In this experimental group, a fermented probiotic agent, the preparation raw materials of the fermented probiotic agent are calculated by weight, including 3 parts of compound probiotic powder, 13 parts of Psoralea corylifolia, 10 parts of Morinda officinalis, 39 parts of straw, 8 parts of soybean meal, 7 parts of trehalose, and 20 parts of deionized water; the rest are the same as those in experimental group 5.

[0054] Experimental group 9

[0055] In this experimental group, a fermented probiotic agent, the preparation raw materials of the fermented probiotic agent are calculated by weight, including 4 parts of compound probiotic powder, 15 parts of Psoralea corylifolia, 8 parts of Morinda officinalis, 42 parts of straw, 5 parts of soybean meal, 8 parts of trehalose, and 18 parts of deionized water; the rest are the same as those in experimental group 5.

[0056] Control group 1

[0057] In this control group, a fermented probiotic agent, the preparation raw materials of the fermented probiotic agent are calculated by weight, including 3 parts of compound probiotic powder, 13 parts of Crataegus pinnatifida, 10 parts of Morinda officinalis, 39 parts of straw, 8 parts of soybean meal, 7 parts of trehalose, and 20 parts of deionized water; the rest are the same as those in experimental group 8.

[0058] Control group 2

[0059] In this control group, a fermented probiotic agent, the preparation raw materials of the fermented probiotic agent are calculated by weight, including 3 parts of compound probiotic powder, 13 parts of Psoralea corylifolia, 10 parts of Psychotria serpens, 39 parts of straw, 8 parts of soybean meal, 7 parts of trehalose, and 20 parts of deionized water; the rest are the same as those in experimental group 8.

[0060] Control group 3

[0061] In this control group, a fermented probiotic agent, the preparation raw materials of the fermented probiotic agent are calculated by weight, including 3 parts of compound probiotic powder, 23 parts of Psoralea corylifolia, 39 parts of straw, 8 parts of soybean meal, 7 parts of trehalose, and 20 parts of deionized water; the rest are the same as those in experimental group 8.

[0062] Control group 4

[0063] In this control group, a fermented probiotic agent, the preparation raw materials of the fermented probiotic agent are calculated by weight, including 3 parts of compound probiotic powder, 23 parts of Morinda officinalis, 39 parts of straw, 8 parts of soybean meal, 7 parts of trehalose, and 20 parts of deionized water; the rest are the same as those in experimental group 8.

[0064] Control group 5

[0065] In this control group, a fermented probiotic agent, the preparation raw materials of the fermented probiotic agent are calculated by weight, including 3 parts of probiotic powder, 13 parts of Psoralea corylifolia, 10 parts of Morinda officinalis, 39 parts of straw, 8 parts of soybean meal, 7 parts of trehalose, and 20 parts of deionized water;

[0066] Among them, the probiotic powder is Bacillus licheniformis RC-JZ8322;

[0067] The rest are the same as those in experimental group 8.

[0068] Control group 6

[0069] In this control group, a fermented probiotic agent, the preparation raw materials of the fermented probiotic agent are calculated by weight, including 3 parts of probiotic powder, 13 parts of Psoralea corylifolia, 10 parts of Morinda officinalis, 39 parts of straw, 8 parts of soybean meal, 7 parts of trehalose, and 20 parts of deionized water;

[0070] Among them, the probiotic powder is Lactobacillus plantarum XY-WSW-2325;

[0071] The rest are the same as those in experimental group 8.

[0072] Control group 7

[0073] In this control group, a fermented probiotic agent, the preparation raw materials of the fermented probiotic agent are calculated by weight, including 3 parts of probiotic powder, 13 parts of Psoralea corylifolia, 10 parts of Morinda officinalis, 39 parts of straw, 8 parts of soybean meal, 7 parts of trehalose, and 20 parts of deionized water;

[0074] Among them, the probiotic powder is Lactobacillus acidophilus NCFM;

[0075] The rest are the same as those in experimental group 8.

[0076] Control group 8

[0077] In this control group, a fermented probiotic agent, the raw materials for preparing the fermented probiotic agent are calculated by weight and include 3 parts of compound probiotic powder, 13 parts of Psoralea corylifolia, 10 parts of Morinda officinalis, 39 parts of straw, 8 parts of soybean meal, 7 parts of trehalose, and 20 parts of deionized water;

[0078] Among them, the compound probiotic powder includes Bacillus licheniformis XHL2309, Lactobacillus plantarum BN1395, and Lactobacillus acidophilus NCFM with a mass ratio of 1:1:1;

[0079] The rest are the same as those in experimental group 8.

[0080] Control group 9

[0081] In this control group, a fermented probiotic agent, the raw materials for preparing the fermented probiotic agent are calculated by weight and include 3 parts of compound probiotic powder, 13 parts of Psoralea corylifolia, 10 parts of Morinda officinalis, 39 parts of straw, 8 parts of soybean meal, 7 parts of trehalose, and 20 parts of deionized water;

[0082] Among them, the compound probiotic powder includes Bacillus licheniformis RC-JZ8322, Lactobacillus plantarum BN1395, and Lactobacillus acidophilus NCFM with a mass ratio of 1:1:1;

[0083] The rest are the same as those in experimental group 8.

[0084] Control group 10

[0085] In this control group, a fermented probiotic agent, the raw materials for preparing the fermented probiotic agent are calculated by weight and include 3 parts of compound probiotic powder, 13 parts of Psoralea corylifolia, 10 parts of Morinda officinalis, 39 parts of straw, 8 parts of soybean meal, 7 parts of trehalose, and 20 parts of deionized water;

[0086] Among them, the compound probiotic powder includes Bacillus licheniformis XHL2309, Lactobacillus plantarum XY-WSW-2325, and Lactobacillus acidophilus NCFM with a mass ratio of 1:1:1;

[0087] The rest are the same as those in experimental group 8.

[0088] III. Experiment

[0089] Select 80 healthy and disease-free yellow cattle with a body weight of 120 kg ± 3 kg, randomly divide them into 20 groups, with 2 replicates in each group and 2 sheep in each replicate, namely experimental groups 1-9, control groups 1-10, and a blank group. In experimental groups 1-9 and control groups 1-10, the fermented probiotic agent of the present application is added to the basal diet at a ratio of 6 wt%. The fermented probiotic agent of the present application is not added to the blank group. The composition and nutritional level of the basal diet for the experiment are shown in Table 2. The yellow cattle in each group are allowed to freely eat and drink water. The experimental period is 120 days. During the experiment, the feeding and management methods of each group are exactly the same, and immunization, deworming, and daily disinfection are carried out according to the conventional management measures of the farm.

[0090] Table 2 Composition and nutritional levels of the basal diet

[0091]

[0092]

[0093] Note: The premix provides Fe 40 mg, Cu 25 mg, Mn 15 mg, Zn 20 mg, I 0.5 mg, Se 0.3 mg, Co 0.2 mg, VA 6000 IU, VD3 1500 IU, and VE 30 IU per kilogram of diet.

[0094] Statistical analysis of animal weight gain test data: The average daily gain, average daily feed intake (7.23 ± 0.47 [kg / (head·d)]), and feed-to-gain ratio (feed-to-gain ratio = average daily feed intake / average daily gain) data of yellow cattle from 0 to 120 days were statistically analyzed. The results are shown in Table 3:

[0095] Table 3 Statistical analysis of animal weight gain test data

[0096]

[0097] On the 120th day of the experiment, 10 mL of blood was collected from the jugular vein of yellow cattle using a disposable syringe and placed in a 15 mL centrifuge tube. The tube was centrifuged at 5000 r / min for 10 min, and the supernatant was the serum sample. The immunoglobulin A (IgA) and immunoglobulin G (IgG) indices in the serum sample were measured sequentially using kits from Nanjing Jiancheng Bioengineering Institute. The data are shown in Table 4:

[0098] Table 4 Statistical analysis of test data on serum immune indices of yellow cattle

[0099]

[0100]

[0116] Experimental conclusion: According to the data statistics results, it can be known that when the fermented probiotics in experimental groups 5, 8, and 9 are added to the basal diet as feed additives, they can promote the growth of livestock and improve the feed utilization rate, with significant effects. At the same time, they can also increase the contents of immunoglobulin A (IgA) and immunoglobulin G (IgG) in the serum of yellow cattle. Immunoglobulin A (IgA) and immunoglobulin G (IgG) play important functions in the immune system of animals. Thus, it can be shown that the fermented probiotics in experimental groups 5, 8, and 9 of the present invention also have the effect of improving the immune function of yellow cattle, and the effects of the fermented probiotics in experimental groups 1, 2, and 3 are second best;

[0117] Through the control experiment, it can be known that using wheat straw and peanut straw as fermentation substrates, adding traditional Chinese medicines Psoralea corylifolia and Morinda officinalis, and fermenting and preparing a fermented probiotic agent with a compound probiotic powder (Bacillus licheniformis RC-JZ8322, Lactobacillus plantarum XY-WSW-2325, Lactobacillus acidophilus NCFM with a mass ratio of 1:1:1), it has excellent effects in promoting the growth of livestock, improving the feed utilization rate, and enhancing the immune function of livestock. It is speculated that there is a synergistic effect between the compound probiotic powder and wheat straw, peanut straw, as well as traditional Chinese medicines Psoralea corylifolia and Morinda officinalis.

[0118] The above tests only represent several implementation modes of the present invention, and the description is relatively specific and detailed, but it should not be construed as a limitation to the scope of the present invention patent. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present invention, several modifications and improvements can still be made, and these all belong to the protection scope of the present invention.

Claims

1. A preparation method of a fermented probiotic agent, characterized in that: The specific steps are as follows:

1. Weigh each raw material by weight, including 2-4 parts of compound probiotic powder, 10-15 parts of psoralea corylifolia, 8-13 parts of Morinda officinalis, 35-42 parts of straw, 5-10 parts of soybean meal, 6-8 parts of trehalose, and 18-24 parts of deionized water; 2. Fully mix the composite probiotic powder, trehalose and deionized water, perform anaerobic closed culture to obtain activated bacterial solution for later use; 3. Dry and grind Psoralea corylifolia, Morinda officinalis, straw and soybean meal respectively, mix them evenly after passing through a 100-mesh sieve, and add them to the activated bacterial solution. After anaerobic sealed fermentation, vacuum freeze-drying is performed to obtain the fermented probiotic agent.

2. The preparation method of a fermented probiotic agent according to claim 1, characterized in that: In steps 1 and 2, the composite probiotic powder comprises Bacillus licheniformis, Lactobacillus plantarum, and Lactobacillus acidophilus NCFM in a mass ratio of 1:1:1; The Bacillus licheniformis is RC-JZ8322, and the Lactobacillus plantarum is XY-WSW-2325; The viable counts of both Bacillus licheniformis and Lactobacillus plantarum are 6.2 - 6.8×10 7 cfu / g; the viable count of Lactobacillus acidophilus NCFM is 7.3 - 7.8×10 8 cfu / g.

3. The preparation method of a fermented probiotic agent according to claim 1, characterized in that: The straw includes at least one of wheat straw, rape straw and peanut straw.

4. The preparation method of a fermented probiotic agent according to claim 1, characterized in that: In step 2, the anaerobic closed culture temperature is 32° C. to 38° C. and the time is 20 to 28 hours.

5. The preparation method of a fermented probiotic agent according to claim 1, characterized in that: In step three, the anaerobic sealed fermentation temperature is 30°C to 40°C, the fermentation humidity is 30% to 40%, and the fermentation time is 5 to 7 days.

6. A fermented probiotic agent, characterized in that: The method is prepared by any one of claims 1 to 5.

7. Use of the fermented probiotic agent as claimed in claim 6 in promoting the growth of livestock and improving the immune function of livestock.

8. The use according to claim 7, wherein the fermented probiotic agent is added to feed as a feed additive to promote animal weight gain, improve animal feed utilization, and increase the content of immunoglobulin A and immunoglobulin G in animal serum.

9. The use according to claim 8, wherein the amount of the fermented probiotic added to the feed is ≥ 6wt%.

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