Compound microbial agent for rapid decomposition of excrement and application of compound microbial agent

Abstract

The invention discloses a compound microbial agent for rapid decomposition of excrement and application of the compound microbial agent, and belongs to the technical field of microbial application. The microbial agent comprises sporotrichum thermophile, phanerochaete chrysosporium, bacillus amyloliquefaciens, bacillus mucilaginosus and pediococcus acidilactici. The viable bacterium concentration of each microbial agent in the compound microbial agent is greater than (1.0-3.0) * 10 < 8 > CFU / mL or (1.0-3.0) * 10 < 8 > CFU / g. The method can be directly applied to cow dung fermentation treatment under the condition of not adding any structural auxiliary materials, and shows good heating capacity, decomposition effect and product quality. In the fermentation process, a pile enters a high-temperature stage on the fourth day and reaches the highest temperature on the sixth day, the high-temperature stage is maintained for 10 days, decomposition is completed within 20 days, and a fermentation product is free of peculiar smell, uniform in structure and good in stability. The method is easy and convenient to operate, high in adaptability and suitable for in-situ rapid treatment of livestock farms or agricultural waste, and has good application and popularization prospects.

Description

Technical Field

[0001] The present invention belongs to the technical field of microbial applications, and particularly relates to a composite microbial agent for rapid composting of feces and its uses. Background Art

[0002] Cow dung is a common organic waste with high moisture content and high nitrogen. The traditional treatment method mainly focuses on aerobic composting. Although it can improve the resource utilization efficiency, it often faces problems such as slow temperature rise, long fermentation cycle, uneven composting, and obvious odor in practical applications.

[0003] To improve the fermentation efficiency, existing research mostly uses functional microbial agents for inoculation, but usually only single strains or dual-bacteria combinations, with limited functional coverage and difficult to support stable operation throughout the fermentation cycle. In addition, due to the existence of a certain proportion of easily degradable substances and excessive moisture in the cow dung raw material, in order to optimize the structure and carbon-nitrogen ratio, traditional composting techniques often need to add additional dry high-carbon materials such as straw and sawdust as auxiliary materials. This process increases the complexity of material handling and labor input, restricting the wide promotion of the technology in scenarios such as small and medium-sized farms and field plots.

[0004] Therefore, developing a composite microbial preparation that can be directly applied to cow dung raw materials without relying on exogenous auxiliary materials to achieve a fermentation system with rapid temperature rise, high-efficiency composting, stable process, and odorless products is an urgent problem to be solved in the current livestock manure resource utilization technology. Summary of the Invention

[0005] Aiming at the above deficiencies in the prior art, the present invention provides a composite microbial agent for rapid composting of feces and its uses, which can directly act on cow dung raw materials without adding structural auxiliary materials such as straw, achieving the goals of rapid temperature rise, stable composting, odorless process, and high-quality products, thereby simplifying the treatment process, reducing the operation cost, and enhancing the practical application efficiency and promotion potential of cow dung composting.

[0006] To achieve the above object, the technical solution adopted by the present invention to solve its technical problems is:

[0007] The object of the present invention is to provide a composite microbial agent for rapid composting of feces, which includes Sporotrichum thermophile, Phanerochaete chrysosporium, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Pediococcus acidilactici; the viable bacteria concentration of each microbial agent in the composite microbial agent is greater than (1.0 - 3.0) × 10 8 CFU / mL or (1.0 - 3.0) × 10 8 CFU / g.

[0008] Among them, Thermomyces lanuginosus was purchased from Shanghai Shisheng Technology Co., Ltd.; Phanerochaete chrysosporium, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Pediococcus acidilactici were purchased from Beijing Biological Preservation Co., Ltd.

[0009] Furthermore, the compound microbial inoculant is a liquid preparation or a solid preparation.

[0010] Furthermore, the compound microbial inoculant is a liquid preparation; the Thermomyces lanuginosus, Phanerochaete chrysosporium, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Pediococcus acidilactici are respectively formed into bacterial solutions, and each bacterial solution is mixed at a volume ratio of 0.5 - 1:0.5 - 1:0.5 - 1.5:1 - 1.5:0.5 - 1.

[0011] Furthermore, the volume ratio of each bacterial solution is 1:1:1:1:1.

[0012] Furthermore, the feces are animal feces, such as cow dung, horse dung, chicken manure, duck manure, etc.

[0013] Another object of the present invention is to provide a bacterial suspension, which comprises the above - mentioned compound microbial inoculant.

[0014] Another object of the present invention is to provide a fermentation product, which comprises the fermentation supernatant or fermentation metabolite produced by the fermentation of the above - mentioned compound microbial inoculant.

[0015] Another object of the present invention is to provide a preparation for rapid composting of feces, which comprises the above - mentioned compound microbial inoculant or bacterial suspension.

[0016] Another object of the present invention is to provide the use of the above - mentioned compound microbial inoculant in promoting the rapid composting of feces.

[0017] Another object of the present invention is to provide a method for promoting the rapid composting of feces, which uses feces as the only fermentation raw material, then inoculates the above - mentioned compound microbial inoculant, bacterial suspension, fermentation product or preparation, and performs stacking fermentation after inoculation.

[0018] Another object of the present invention is to provide the use of the above - mentioned compound microbial inoculant, bacterial suspension, fermentation product or preparation in promoting plant growth.

[0019] The beneficial effects of the present invention:

[0020] 1. Without structural auxiliary materials, significantly simplifying the fermentation process

[0021] In traditional cow dung composting, in order to adjust the carbon-nitrogen ratio and improve the structural properties of the materials, it is often necessary to add high-carbon materials such as straw and sawdust additionally, which increases the links of raw material preparation, ratio regulation and manual turning. Especially in areas where straw resources are scarce or difficult to crush, the treatment efficiency and cost control are severely restricted.

[0022] However, the composite microbial inoculant of the present invention has good system regulation ability and can directly act on cow dung raw materials. Without adding any structural auxiliary materials, it can achieve stable temperature rise and composting process, and has excellent fermentation effect, verifying its strong adaptability to raw materials, simple process and convenient management. While reducing the process steps, it greatly improves the adaptability and on-site practicality of the technology.

[0023] 2. The strain combination is scientific and rigorous, and the functions cooperate clearly

[0024] The inoculant of the present invention is composed of five microorganisms with clear functional positions, which are responsible for the key links in the composting process respectively:

[0025] Sporotrichum thermophile is used to initiate and maintain high temperature; Phanerochaete chrysosporium participates in the degradation of structures such as lignin and cellulose, improving the degree of compost maturity and structure decomposition in the later stage of composting; Bacillus amyloliquefaciens mainly acts in the early stage of fermentation, degrading the easily degradable carbon source in cow dung, providing starting energy for the rapid reproduction and temperature rise of microorganisms, and helping to release carbon source in the initial stage of fermentation; Bacillus mucilaginosus has the ability to dissolve phosphorus and potassium, enhancing nutrient release; Pediococcus acidilactici regulates the pH of the compost pile through acid production, inhibits the growth of spoilage bacteria and ammonia release, and significantly reduces ammonia volatilization and odor release. This combination covers the core links of multiple composting processes such as temperature rise initiation, degradation promotion, system stability, and odor control, making up for the problems of overlapping functions and single action of traditional two-strain or three-strain combinations, and the overall synergy efficiency is higher.

[0026] The carbon-nitrogen ratio (C:N) of the product of cow dung compost inoculated with the inoculant combination of the present invention is the lowest, and the pH is also the lowest. Specifically, the carbon-nitrogen ratio (C:N) of the cow dung compost product is reduced from about 18.42 initially to 11.2, a decrease of about 40%. The pH value is reduced from 8.32 to 7.75, a decrease of 6%. The effective nitrogen and total phosphorus contents of the final fermentation product inoculated with the inoculant of the present invention are the highest.

[0027] 3. The fermentation process is stable and the composting effect is remarkable

[0028] The inoculant of the present invention is in a small compost pile (50×70×50cm 3) It is possible to achieve a rapid increase in the temperature of the compost heap, and it can enter the high-temperature stage (above 50°C) on the 4th day, reaching the highest temperature of 63°C on the 6th day. The entire fermentation cycle is controlled within 20 days. The pH of the product decreases, the C / N ratio decreases significantly, the structure becomes loose, the color deepens, and the germination index (GI) of the seeds exceeds 115%, indicating sufficient composting, low toxicity, and good agricultural safety. Stable composting can still be achieved in a small-volume fermentation system that is not conducive to maintaining temperature, demonstrating the good high efficiency and environmental adaptability of this bacterial agent combination, and it is suitable for popularization and application under different scale conditions.

[0029] For the control group of bacterial agent combinations, the fastest one entered the high-temperature stage on the 6th day, and reached the highest temperature successively on the 10th day. Moreover, the highest temperature of the compost of other bacterial agent combinations could only reach about 55°C. After inoculating the bacterial agent combination of the present invention, the composting was completed within 21 days, the temperature of the compost heap naturally dropped, and the odor disappeared. The composting was completed 2 - 5 days earlier than the control group, and the fermentation efficiency was significantly improved.

[0030] 4. The odor is significantly reduced, and it is more environmentally friendly

[0031] Cow dung has a high nitrogen content, and ammonia volatilization and odor release are very likely to occur during the fermentation process. Although traditional bacterial agents have made progress in degradation ability, they generally lack systematic regulation of the microecology of the compost heap and ammonia volatilization.

[0032] In the present invention, Pediococcus acidilactici is particularly introduced. By producing acid in the early stage of fermentation to adjust the pH, inhibit the growth of putrefactive bacteria, and reduce the ammonia volatilization rate, the simultaneous promotion of "deodorization + composting" is achieved, which is of great significance for improving the odor pollution problem in the process of treating livestock and poultry waste, and is particularly suitable for use in scenarios close to residential areas.

[0033] 5. The nutrient retention rate of the product is high, and it has good agricultural value

[0034] After the fermentation is completed, the total nitrogen (TN) content in cow dung increases. This is mainly due to the large degradation of carbon sources, the concentration of organic matter, and the enrichment effect of microbial residues during the fermentation process, showing a good nitrogen retention effect. At the same time, the structure of the product is loose, which is conducive to soil aeration. Forage grass experiments show that the fresh weight of the above-ground part increases significantly after its application, and it has both fertilizer efficiency and biological stimulation performance.

[0035] 6. It has strong scalability and is suitable for a variety of actual application scenarios

[0036] The fermentation system of the present invention shows good temperature rise performance and stability under laboratory-scale conditions, and has the feasibility of expanding to pilot-scale and engineering-scale (such as 1.5 - 2m windrow composting, elevated trough composting). The preparation cost of the bacterial agent is moderate, and the inoculation method is simple. It is suitable for various treatment scenarios such as daily composting in farms, household composting in rural areas, and on-site utilization of manure resources.

[0037] 7. The present invention provides a multi-functional composite microbial inoculant combination system that can directly act on cow dung raw materials. Through precise strain configuration and stable fermentation control, it achieves comprehensive technical effects of simplifying operations, improving efficiency, reducing pollution, and enhancing product quality. It not only breaks through the technical bottlenecks of traditional composting treatment's dependence on auxiliary materials and poor fermentation stability, but also provides a new feasible path for the resource utilization of organic waste, with good innovation, practicability, and promotion value. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 It is a curve graph of temperature changes during composting under different microbial inoculant combinations;

[0039] Figure 2 It is a detection graph of the carbon-nitrogen ratio of the compost product after treatment with different microbial inoculants;

[0040] Figure 3 It is a detection graph of the pH of the compost product after treatment with different microbial inoculants;

[0041] Figure 4 It is a detection graph of the available nitrogen content of the compost product after treatment with different microbial inoculants;

[0042] Figure 5 It is a detection graph of the total phosphorus content of the compost product after treatment with different microbial inoculants;

[0043] Figure 6 It is the germination index (GI) of the compost product after treatment with different microbial inoculants;

[0044] Figure 7 It is the strong seedling index (SVI) of forage grass after applying the compost product treated with different inoculants;

[0045] Figure 8 It is a detection graph of the fresh weight of forage grass after applying the compost product treated with different inoculants. DETAILED DESCRIPTION OF THE INVENTION

[0046] The following describes the specific embodiments of the present invention to facilitate those skilled in the art to understand the present invention. However, it should be clear that the present invention is not limited to the scope of the specific embodiments. For those of ordinary skill in the art, as long as various changes are within the spirit and scope of the present invention defined and determined by the appended claims, these changes are obvious, and all inventions made using the concept of the present invention are within the scope of protection.

[0047] The bacterial solutions of Bacillus subtilis, Aspergillus niger, Trichoderma harzianum, and Trichoderma reesei used in the present invention are all purchased from Beijing Biological Preservation Co., Ltd.

[0048] Example 1

[0049] A compound microbial inoculant for rapid composting of feces, which is a liquid inoculant and includes Sporotrichum thermophile, Phanerochaete chrysosporium, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Pediococcus acidilactici;

[0050] The viable bacteria concentration of each inoculant in the compound microbial inoculant is 5.0×10 8 CFU / mL, and the bacterial liquids formed by each inoculant are mixed in a volume ratio of 1:1:1:1:1.

[0051] Example 2

[0052] A compound microbial inoculant for rapid composting of feces, which is a liquid inoculant and includes Sporotrichum thermophile, Phanerochaete chrysosporium, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Pediococcus acidilactici;

[0053] The viable bacteria concentration of each inoculant in the compound microbial inoculant is 4.5×10 8 CFU / mL, and the bacterial liquids formed by each inoculant are mixed in a volume ratio of 1:1:1.5:1:1.

[0054] Example 3

[0055] A compound microbial inoculant for rapid composting of feces, which is a liquid inoculant and includes Sporotrichum thermophile, Phanerochaete chrysosporium, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Pediococcus acidilactici;

[0056] The viable bacteria concentration of each inoculant in the compound microbial inoculant is 2.0×10 9 CFU / mL, and the bacterial liquids formed by each inoculant are mixed in a volume ratio of 0.5:0.5:0.5:1:0.5.

[0057] Example 4

[0058] A compound microbial inoculant for rapid composting of feces, which is a liquid inoculant and includes Sporotrichum thermophile, Phanerochaete chrysosporium, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Pediococcus acidilactici;

[0059] The viable bacteria concentration of each inoculant in the compound microbial inoculant is 3.0×10 9 CFU / mL, and the bacterial liquids formed by each inoculant are mixed in a volume ratio of 0.6:0.5:1:1.2:0.8.

[0060] Control group

[0061] To further illustrate the efficacy of the compound microbial inoculant of the present invention, the following control group scheme is provided. The dosage ratio and viable bacteria concentration of each strain in the control group scheme are the same as those in Example 1. The specific control group inoculant scheme is as follows:

[0062] Other microbial agents 1: Bacillus subtilis + Phanerochaete chrysosporium + Aspergillus niger + Bacillus mucilaginosus + Pediococcus acidilactici;

[0063] Other microbial agents 2: Bacillus subtilis + Phanerochaete chrysosporium + Bacillus amyloliquefaciens + Trichoderma harzianum + Pediococcus acidilactici;

[0064] Other microbial agents 3: Bacillus subtilis + Trichoderma reesei + Aspergillus niger + Bacillus mucilaginosus + Pediococcus acidilactici;

[0065] Other microbial agents 4: Bacillus subtilis + Trichoderma reesei + Aspergillus niger + Trichoderma harzianum + Pediococcus acidilactici;

[0066] Other microbial agents 5: Bacillus subtilis + Trichoderma reesei + Bacillus amyloliquefaciens + Trichoderma harzianum + Pediococcus acidilactici;

[0067] Other microbial agents 6: Bacillus subtilis + Phanerochaete chrysosporium + Aspergillus niger + Trichoderma harzianum + Pediococcus acidilactici;

[0068] Other microbial agents 7: Sporotrichum thermophile + Trichoderma reesei + Bacillus amyloliquefaciens + Bacillus mucilaginosus + Pediococcus acidilactici;

[0069] Other microbial agents 8: Sporotrichum thermophile + Phanerochaete chrysosporium + Aspergillus niger + Bacillus mucilaginosus + Pediococcus acidilactici;

[0070] Other microbial agents 9: Sporotrichum thermophile + Phanerochaete chrysosporium + Bacillus amyloliquefaciens + Trichoderma harzianum + Pediococcus acidilactici;

[0071] Other microbial agents 10: Sporotrichum thermophile + Trichoderma reesei + Aspergillus niger + Bacillus mucilaginosus + Pediococcus acidilactici;

[0072] Other microbial agents 11: Sporotrichum thermophile + Trichoderma reesei + Aspergillus niger + Trichoderma harzianum + Pediococcus acidilactici;

[0073] Other microbial agents 12: Sporotrichum thermophile + Trichoderma reesei + Bacillus amyloliquefaciens + Trichoderma harzianum + Pediococcus acidilactici;

[0074] Other microbial agents 13: Sporotrichum thermophile + Phanerochaete chrysosporium + Aspergillus niger + Trichoderma harzianum + Pediococcus acidilactici.

[0075] Finally, cow dung without adding any microbial agents was used as the blank control group.

[0076] Test examples

[0077] 1. The composite microbial agents in Example 1 and the control group were used for cow dung fermentation testing, and the specific process was as follows:

[0078] S1: Raw material preparation and test frame arrangement

[0079] S11: Select fresh cow dung from a conventional farm as the only fermentation raw material, without adding structural auxiliary materials such as straw and sawdust. To improve the repeatability of the experiment and the comparability between treatments, the whole batch of cow dung is manually turned over three times in advance to mix it thoroughly and ensure the consistency of the raw materials used in each experimental group. This operation helps to reduce the interference of the differences in the physical and chemical properties of the raw materials on the inoculation effect and enhances the reliability of the experimental data.

[0080] S12: Adjust the moisture content to 55% - 65%, with the standard that when squeezed by hand, it forms a ball without dripping water and loosens when released.

[0081] S13: Load the cow dung into a fixed frame of 50 cm × 70 cm × 50 cm (length × width × height). A total of 45 frames are prepared for different inoculant treatments and repeated experiments.

[0082] S2: Preparation of the bacterial solution and inoculation

[0083] S21: Prepare the bacterial solution according to the protocols recorded in Example 1 and the control group. All bacterial solutions are stored refrigerated at 4°C before inoculation and activated at room temperature for 1 hour before inoculation to ensure the activity of the bacteria during inoculation.

[0084] S2:2: According to the dosage of 0.5% of the wet weight of the cow dung (0.5 mL of the bacterial solution is inoculated per 100 g of cow dung), evenly spray the prepared compound bacterial solution on the surface of the cow dung.

[0085] S23: Manually mix the materials with a small shovel or by manual turning to ensure that the bacterial solution is fully contacted and distributed with the raw materials. This operation is only carried out once, and no additional inoculant is added during the subsequent fermentation process.

[0086] S3: Management of stacking fermentation

[0087] S31: Naturally stack the inoculated cow dung in the frame and place it in a well-ventilated environment. During fermentation, no artificial temperature control or air-blowing device is used, and it completely relies on natural ventilation and heat accumulation.

[0088] S32: Within the first 10 days, manually turn the pile once every 2 - 3 days to promote ventilation and balance the temperature. The frequency of turning the pile in the later stage is appropriately reduced according to the temperature change of the pile body.

[0089] S33: When the temperature of the pile body exceeds 65°C, carry out the turning pile operation in advance to prevent local overheating from inhibiting the activity of the bacteria.

[0090] S4: Recording of temperature changes and determination of maturity

[0091] S41: After inoculation and fermentation, measure the temperature daily. The temperature is measured using a center-inserted needle thermometer, and the center temperature of the pile is measured at 8 am and 4 pm every day.

[0092] S42: The criteria for completed composting include the following indicators: the temperature of the compost pile drops below 30 °C, the color of the fermentation product deepens, and the structure becomes loose; the peculiar smell disappears, presenting a natural soil smell.

[0093] S43: Take samples of the mature fermentation product and send them to the laboratory to measure indicators such as pH, available nitrogen (ammonium nitrogen and nitrate nitrogen), available phosphorus, organic matter, total carbon, total nitrogen, total phosphorus, and germination index.

[0094] The detection methods refer to national and industry standards such as "NY / T 525-2021 Organic Fertilizer" and "GB / T 19524.1-2004", and the results are shown in Figures 1 to 6 .

[0095] The present invention verifies the fermentation performance and application potential of the composite microbial inoculant combination of the present invention under the condition of a small compost pile.

[0096] As Figure 1 shown, compared with the control inoculant in the comparative example, the composite microbial inoculant treatment group prepared by the present invention has a faster temperature rise and a longer duration of the high-temperature period (≥55 °C), indicating that it has stronger organic matter degradation ability and composting fermentation activity. Figure 2 The C / N detection results in

[0097] Figure 3 also further confirm that the composite microbial inoculant constructed by the present invention has better effects in organic matter degradation. Figure 4 and Figure 5 show that the pH of the compost product treated with the composite microbial inoculant constructed by the present invention is the lowest, significantly lower than most of the controls, indicating that the final product obtained by the treatment with the composite microbial inoculant of the present invention is more stable and more suitable for plant growth. In addition, the contents of available nitrogen and total phosphorus in the compost product after treatment with the composite microbial inoculant of the present invention are the highest (

[0098] According to Figure 6 the detection results, the germination index of the composite microbial inoculant treatment group prepared by the present invention is greater than 100%, indicating that its compost product has low toxicity and is fully composted, and has met the conditions for safe use in plants. And its GI value is significantly higher than that of the control group, further indicating that the composite microbial inoculant prepared by the present invention has good plant growth promotion potential, which helps to improve the agricultural application value of the compost product.

[0099] Based on the above results, the compost heap treated with the compound microbial inoculant constructed in the present invention had a rapid temperature rise during fermentation. It entered the high-temperature period (>50 °C) on the 4th day and reached the highest temperature of 64 °C on the 6th day, which was 2 - 4 days earlier than the other inoculant combinations in the control group on average. The C / N of the fermentation product decreased from the initial 18.42 to 11.2, the pH dropped to 7.75, the structure was loose, without odor, the GI reached 112%, and the composting period was 20 days.

[0100] 2. Monitoring of agricultural effects

[0101] S1: Take the mature compost obtained from each treatment group above for potting pasture experiments. Apply 10% (by volume) of the mature compost obtained from the corresponding treatment in the previous composting experiment into the soil. After thoroughly mixing it with the soil, fill it into pots, with 5 kg of soil in each pot and 20 oat seeds sown in each pot.

[0102] S2: During the growth period of oats, unified management is adopted and watering is carried out at the appropriate time. Check the emergence situation of each pot every day at the initial stage of the experiment and record it to calculate the emergence rate. After 7 days of germination, count the number of actually germinated seeds in each pot, measure the above-ground part and root length of each seedling, and calculate the average total seedling length. Calculate the Seedling Vigor Index (SVI) according to the following formula (the results are shown in Figure 7 ):

[0103] SVI = germination rate (%) × average seedling length (cm)

[0104] S3: After 20 days of planting, uniformly collect the plants in each pot and weigh the fresh weight of the above-ground part. The results are shown in Figure 8 .

[0105] As Figure 7 and Figure 8 shown, in the pasture experiment, the Seedling Vigor Index and the fresh weight of forage obtained from the compost product treated with the compound microbial inoculant prepared in the present invention were the highest, indicating that the compound microbial inoculant prepared in the present invention helps to improve the growth potential after seed germination, has a good growth-promoting effect, and also has obvious advantages in improving plant productivity.

[0106] The compound microbial inoculant combination proposed in the present invention has good high-temperature adaptability, microecological regulation ability and system synergy efficiency. The fermentation process is stable, the degree of compost maturity is high, and the agricultural value of the product is excellent, with good applicability and promotion prospects. Moreover, according to the test results, the compound microbial inoculant constructed in the present invention is overall superior to the other inoculant combinations in the control group in terms of temperature rise efficiency, odor control, degree of compost maturity and growth-promoting effect, reflecting the outstanding advantages of the compound microbial inoculant of the present invention in multi-functional synergy and system stability, and having strong practical application value and promotion potential.

[0107] Finally, it should be noted that the above specific embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail with reference to the examples, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced without departing from the spirit and scope of the technical solutions of the present invention, and they should all be covered within the scope of the claims of the present invention.

Claims

1. A compound microbial inoculum for rapid composting of feces, characterized in that, It includes Thermosporothrix thermophila, Phanerochaete chrysosporium, Bacillus amyloliquefaciens, Bacillus mucilaginosus and Pediococcus acidilactici; the viable bacteria concentration of each bacterial agent in the composite microbial inoculum is greater than (1.0 - 3.0)×10 8 CFU / mL or (1.0 - 3.0)×10 8 CFU / g.

2. The composite microbial inoculum according to claim 1, characterized in that, The composite microbial inoculant is a liquid preparation or a solid preparation.

3. The composite microbial inoculant according to claim 2, wherein The composite microbial inoculant is a liquid preparation; the Thermophilic Sporotrichum thermophile, Phanerochaete chrysosporium, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Pediococcus acidilactici are respectively formed into bacterial liquids, and each bacterial liquid is mixed in a volume ratio of 0.5-1:0.5-1:0.5-1.5:1-1.5:0.5-1.

4. The composite microbial inoculum according to claims 1 to 3, characterized in that, The feces are animal feces.

5. A bacterial suspension, characterized in that, It includes the composite microbial inoculant according to any one of claims 1 to 4.

6. A fermentation product, characterized in that, It includes the fermentation supernatant or fermentation metabolite produced by the fermentation of the composite microbial inoculant according to any one of claims 1 to 4.

7. A preparation for rapid composting of feces, characterized in that, It includes the composite microbial inoculant according to any one of claims 1 to 4, or the bacterial suspension according to claim 5.

8. Use of the composite microbial inoculant according to any one of claims 1 to 4, the bacterial suspension according to claim 5, the fermentation product according to claim 6, or the preparation according to claim 7 in promoting the rapid composting of feces.

9. A method for promoting rapid composting of feces, characterized in that, Using feces as the sole fermentation raw material, then inoculating the composite microbial inoculant according to any one of claims 1 to 4, the bacterial suspension according to claim 5, the fermentation product according to claim 6, or the preparation according to claim 7, and performing stacking fermentation after inoculation.

10. Use of the composite microbial inoculant according to any one of claims 1 to 4, the bacterial suspension according to claim 5, the fermentation product according to claim 6, or the preparation according to claim 7 in promoting plant growth.

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