Stem cell supernatant cryopreservation device

Abstract

The invention relates to the technical field of cell cryopreservation, in particular to a stem cell supernatant cryopreservation device which comprises a box body, a first supporting table is fixedly connected to the inner side wall of the box body, a first supporting column is fixedly connected to the bottom end of the first supporting table, the first supporting column is sleeved with and rotationally connected with a connecting rod, and lantern rings are fixedly connected to the two ends of the connecting rod; a second supporting column is fixedly connected into the lantern ring close to one end of the first supporting column, a freezing cover is hinged to the lantern ring away from one end of the first supporting column, and a pulley is in sliding fit with the top of the first supporting table and rotationally connected with the freezing cover. A refrigeration assembly is arranged on the freezing cover, a storage assembly is arranged in the opening, and a suction assembly is arranged in the box body. The freezing cover covers the top of the storage drawer, the refrigerator at the top of the freezing cover is used for refrigerating the freezing cover, and the freezing cover is used for uniformly freezing the stem cell supernate in the storage drawer, so that the freezing storage effect of the stem cell supernate is improved, and the generation of ice crystals in stem cells is reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of cell cryopreservation, and particularly relates to a cryopreservation device for stem cell supernatant. Background Art

[0002] Stem cell supernatant refers to the remaining liquid after removing stem cells and other cell components from the stem cell culture medium. This liquid consists of three parts: culture medium, cytokines, and exosomes. Stem cells release substances such as cytokines and growth factors, which can stimulate the proliferation and differentiation of surrounding cells. In short, the supernatant is rich in a large number of cell active substances, and its main function is to accelerate repair, promote cell activation, and improve cell survival rate, thereby achieving the effect of "reverse growth".

[0003] There may be a long interval between cell collection and clinical application, while the storage time limit of stem cell supernatant is relatively short. In order to extend its storage time limit or conduct long-term storage, it is necessary to freeze and store the stem cell supernatant. The cryopreservation of stem cell supernatant has high requirements for temperature control.

[0004] Currently, the existing cryopreservation devices for stem cell supernatant have certain limitations in temperature control accuracy when cryopreserving stem cell supernatant. The freezing effect on the stem cell supernatant is uneven, which easily leads to the formation of ice crystals in the stem cells. Therefore, it is necessary to propose a cryopreservation device for stem cell supernatant. Summary of the Invention

[0005] To solve the above problems, the present invention provides a cryopreservation device for stem cell supernatant. By covering the storage drawer with a freezing cover and using the cooler on the top of the freezing cover to cool the freezing cover, the stem cell supernatant in the storage drawer is evenly frozen, improving the cryopreservation effect of the stem cell supernatant and reducing the formation of ice crystals in the stem cells.

[0006] To achieve the above object, the technical solution of the present invention is as follows: A cryopreservation device for stem cell supernatant, comprising a box body. An opening for communicating with the outside is provided on one side of the box body. A first support platform and a second support platform are fixedly connected to the inner side wall of the box body, and the first support platform is directly above the second support platform.

[0007] A first support column is fixedly connected to the bottom end of the first support platform, far away from the inner wall of the box body. The first support column is sleeved and rotatably connected with a "へ"-shaped connecting rod. Both ends of the connecting rod are fixedly connected with collar rings. A second support column is fixedly connected inside the collar ring near the first support column. The collar ring far away from the first support column is hinged with a freezing cover. A pulley is slidably engaged with the top of the first support platform, and the pulley is rotatably connected with the freezing cover. A refrigeration component for cooling the stem cell supernatant is provided on the freezing cover;

[0008] On the side of the second support platform away from the inner wall of the box body, a third support column is fixedly connected, and a first spring is fixedly connected between the third support column and the second support column.

[0009] A storage component for storing the stem cell supernatant is provided inside the opening, and an attracting component for attracting the storage component is provided on the inner wall of the box body at the end far from the opening.

[0010] The technical principle of the above solution is as follows:

[0011] The storage component located inside the opening of the box body is used to store the stem cell supernatant to be frozen. The refrigeration component on the freezing cover is responsible for providing the necessary low-temperature environment for the stem cell supernatant.

[0012] Through the extrusion of the storage component, the storage component will extrude the freezing cover. When the freezing cover moves, it will drive the connecting rod to rotate, and the freezing cover will slide along the upper surface of the first support platform under the action of the pulley, so that the freezing cover covers the entire storage component.

[0013] A first spring is fixedly connected between the third support column and the second support column. When the freezing cover is closed, that is, during the rotation of the connecting rod, the first spring will be continuously compressed; when the storage component is pulled out, the first spring will undergo elastic deformation to help the freezing cover reset. This design not only improves the convenience of operation but also helps to maintain the stability of the device.

[0014] The above solution has the following beneficial effects:

[0015] 1. The present invention provides a low-temperature environment for the freezing cover through the refrigeration component, and freezes the stem cell supernatant in the storage component through the freezing cover, which is beneficial to the uniform freezing of the stem cell supernatant, thereby reducing the generation of ice crystals in the stem cell supernatant.

[0016] 2. When the freezing cover covers the storage component, the first spring is compressed by the freezing cover, so that the first spring stores energy. When the staff takes out the storage component, the freezing cover will reset to the vertical position under the action of the first spring, which is beneficial to improving the convenience and efficiency of the staff's operation.

[0017] Further, the storage component includes a storage drawer. An iron sheet and an extension plate are fixedly connected to the outer wall of the storage drawer on the side away from the opening of the box body. The extension plate is located below the iron sheet; the attracting component includes an electromagnet, which is fixedly connected to the inner wall of the box body, and a controller is fixedly connected to the outside of the box body. The controller is used to control the start and stop of the electromagnet.

[0018] Beneficial effect: When it is necessary to push the storage drawer into the box body, the electromagnet can be started through the controller. Due to the magnetic force between the electromagnet and the iron sheet, the storage drawer can be easily attracted, making the process of putting the storage drawer more simple and efficient.

[0019] The extension board is located below the iron sheet. When the storage drawer is inserted, the extension board helps to push the freezing cover in advance, enabling the freezing cover to slide on the upper surface of the first support platform through the pulley.

[0020] Furthermore, a second spring is fixedly connected between the electromagnet and the iron sheet.

[0021] Beneficial effect: The second spring can absorb the impact force when the electromagnet attracts or releases the storage drawer, reducing the direct collision between the iron sheet and the electromagnet, thereby prolonging the service life of the device.

[0022] When the electromagnet is powered off, the second spring can provide a certain reset force, which is beneficial to help the storage drawer be smoothly pulled out.

[0023] Furthermore, the refrigeration component includes a refrigerator, which is fixedly connected to the upper surface of the freezing cover, and the controller is used to control the start and stop of the refrigerator.

[0024] Beneficial effect: The refrigerator is directly fixedly connected to the upper surface of the freezing cover, which can ensure the maximization of the heat exchange efficiency between the refrigerant circulation system and the freezing space. This design reduces the energy loss during the heat transfer process, making the refrigeration effect more significant. The close contact between the refrigerator and the freezing cover also helps to reduce the accumulation of condensed water on the freezing cover, thereby keeping the storage drawer dry and clean.

[0025] Furthermore, a temperature sensor is fixedly connected inside the storage drawer, and the controller is used to receive the temperature data from the temperature sensor.

[0026] Beneficial effect: The temperature sensor can real-time monitor the temperature inside the storage drawer, ensuring that the temperature of the stem cell supernatant can be accurately controlled during storage. This precise temperature monitoring helps to improve the freezing effect of the stem cell supernatant.

[0027] Based on the temperature data monitored by the temperature sensor, the controller can adjust the working state of the refrigerator to improve the accuracy of temperature regulation inside the storage drawer.

[0028] Furthermore, a sealing strip for reducing cold air leakage is fixedly connected at the opening.

[0029] Beneficial effect: The sealing strip can closely fit the gap at the opening, effectively reducing the leakage of cold air from the gap. This helps to improve the refrigeration effect inside the box.

[0030] While improving the refrigeration effect inside the box, the sealing strip also significantly improves the sealing performance of the device, reducing the entry of external air, dust, moisture and other impurities into the storage drawer, thus preventing the pollution of the stem cell supernatant.

[0031] Furthermore, an indicator light is fixedly connected to the top of the box body, and the controller is used to control the indicator light to flash to remind the staff when the temperature changes abnormally.

[0032] Beneficial effects: When the temperature in the storage drawer changes abnormally, the indicator light will immediately flash under the control of the controller, providing an immediate visual warning to the staff. This immediate feedback helps the staff take measures quickly and reduces the damage to the stem cell supernatant caused by the unnoticed abnormal temperature.

[0033] Furthermore, an alarm is fixedly connected to the top of the box body, and the controller is used to control the alarm to sound when the temperature changes abnormally.

[0034] Beneficial effects: When the temperature in the storage drawer changes abnormally, the alarm will sound under the control of the controller. When the staff is not at the work site and cannot observe the change of the indicator light in time, the alarm reminds the staff that the temperature in the storage drawer is abnormal.

[0035] Furthermore, the inner wall of the storage drawer is covered with a heat-insulating layer.

[0036] Beneficial effects: The heat-insulating layer is used to reduce heat transfer. The heat-insulating layer is beneficial to reducing the loss of cold air in the storage drawer to the external environment. Therefore, the heat-insulating layer reduces the energy required for the refrigerator to maintain a low temperature and is beneficial to reducing the energy consumption of the refrigerator.

[0037] Furthermore, a partition board for separating the storage drawer from the third support column is fixedly connected to the inner bottom wall of the box body.

[0038] Beneficial effects: The partition board is used to limit the position of the storage drawer in the box body and reduce the direct contact between the storage drawer and the third support column, thereby preventing damage to the third support column.

[0039] The additional aspects and advantages of the present invention will be partly given in the following description, partly become obvious from the following description, or be understood through the practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0040] Figure 1 It is an axonometric view of the stem cell supernatant cryopreservation device in the embodiment of the present invention.

[0041] Figure 2 For the embodiment of the present invention Figure 1 A cross-sectional view of the stem cell supernatant cryopreservation device along A-A in the embodiment.

[0042] Figure 3 For the embodiment of the present invention Figure 1 A cross-sectional view of the stem cell supernatant cryopreservation device along B-B in the embodiment.

[0043] Figure 4For the embodiments of the present invention Figure 1 Cross-sectional view of the cryopreservation device for stem cell supernatant along C-C in the present invention

[0044] The reference numerals in the accompanying drawings of the specification include: 1, box body; 2, storage drawer; 3, alarm; 4, indicator light; 5, temperature sensor; 6, second support platform; 7, third support column; 8, connecting rod; 9, first spring; 10, first support column; 11, first support platform; 12, second support column; 13, freezing cover; 14, collar; 15, pulley; 16, electromagnet; 17, second spring; 18, extension plate; 19, iron sheet; 20, refrigerator; 21, partition board Specific embodiments

[0045] The following is a further detailed description through specific embodiments

[0046] Embodiment 1

[0047] As shown in the attached Figures 1-4 figure: A cryopreservation device for stem cell supernatant includes a box body 1. An opening for communicating with the outside is provided on one side of the box body 1. The inner side wall of the box body 1 is fixedly connected with a first support platform 11 and a second support platform 6 by bolts. The first support platform 11 is located directly above the second support platform 6. One side of the bottom end of the first support platform 11 away from the inner wall of the box body 1 is fixedly connected with a first support column 10 by bolts. The first support column 10 is sleeved and rotatably connected with a "へ"-shaped connecting rod 8. Both ends of the connecting rod 8 are fixedly connected with collars 14 by bolts. A second support column 12 is fixedly connected inside the right collar 14 by bolts. The left collar 14 is hinged with a freezing cover 13. A pulley 15 is slidably fitted on the top of the first support platform 11, and the pulley 15 is rotatably connected with the freezing cover 13; A refrigeration component for refrigerating the stem cell supernatant is provided on the freezing cover 13

[0048] As Figure 2 and Figure 3 shown, one side of the second support platform 6 away from the inner wall of the box body 1 is fixedly connected with a third support column 7 by bolts. A first spring 9 is fixedly connected between the third support column 7 and the second support column 12 by screws

[0049] A storage component for storing stem cell supernatant is provided inside the opening, and an attracting component for attracting the storage component is provided on the inner wall of the box body 1 at the end far from the opening

[0050] As Figure 4As shown in the figure, the storage component includes a storage drawer 2. On the left side of the storage drawer 2, an iron sheet 19 and an extension plate 18 are fixedly connected by screws. The extension plate 18 is located below the iron sheet 19. The attraction component includes an electromagnet 16. The electromagnet 16 is fixedly connected to the inner wall of the box body 1 by screws. A controller is fixedly connected to the outside of the box body 1 by screws. The controller is used to control the startup and shutdown of the electromagnet 16. A second spring 17 is fixedly connected between the electromagnet 16 and the iron sheet 19 by screws.

[0051] The refrigeration component includes a refrigerator 20. The refrigerator 20 is fixedly connected to the upper surface of the freezing cover 13 by bolts. The controller is used to control the startup and shutdown of the refrigerator 20.

[0052] As Figure 2 shown, a temperature sensor 5 is fixedly connected inside the storage drawer 2 by screws. The controller is used to receive the temperature data of the temperature sensor 5.

[0053] As Figure 2 shown, an indicator light 4 is fixedly connected to the top of the box body 1 by screws. The controller is used to control the indicator light 4 to flash to remind the staff when the temperature changes abnormally.

[0054] The specific implementation process is as follows: When the staff needs to freeze the stem cell supernatant, the staff first needs to pull out the storage drawer 2, then put the sealed stem cell supernatant into the storage drawer 2, and then push the storage drawer 2 into the box body 1. At the same time, the staff can start the electromagnet 16 through the controller. During the process of the staff pushing the storage drawer 2 into the box body 1, taking Figure 4 as an example, the extension plate 18 will first abut against the freezing cover 13. The extension plate 18 will push the freezing cover 13 to move upward. When the freezing cover 13 moves upward, it will slide along the surface of the first support platform 11 through the pulley 15. Taking Figure 3 as an example, when the freezing cover 13 slides along the surface of the first support platform 11, the freezing cover 13 will drive the connecting rod 8 to rotate through the collar 14 at the end away from the first support column 10. Taking Figure 2 as an example, when the connecting rod 8 rotates, it will drive the second support column 12 to rotate. Because a first spring 9 is fixedly connected between the second support column 12 and the third support column 7 by bolts, when the second support column 12 rotates, the second support column 12 will squeeze the first spring 9 to store energy in the first spring 9.

[0055] The freezing cover 13 will continue to slide along the upper surface of the first support platform 11 through the pulley 15. When the bottom end of the freezing cover 13 slides above the extension plate 18, the storage drawer 2 will continue to push the freezing cover 13 to slide along the upper surface of the first support platform 11 through the pulley 15.

[0056] Taking Figure 4For example, during the process of pushing the storage drawer 2 into the box body 1, the second spring 17 will continuously store energy under the push of the storage drawer 2, and the electromagnet 16 will continuously generate an attractive force on the iron sheet 19.

[0057] After the storage drawer 2 is completely pushed into the box body 1, the iron sheet 19 abuts against the electromagnet 16, and the freezing cover 13 slides to directly above the storage drawer 2. At this time, the freezing cover 13 will completely cover the storage drawer 2. At this time, the staff can turn on the refrigerator 20 through the controller, cool the freezing cover 13 through the refrigerator 20, and use the freezing cover 13 to evenly freeze the stem cell supernatant in the storage drawer 2.

[0058] During the refrigeration process of the refrigerator 20, the temperature sensor 5 will continuously monitor the temperature in the storage drawer 2 to ensure that the temperature in the storage drawer 2 meets the freezing temperature of the stem cell supernatant.

[0059] When the freezing temperature in the storage drawer 2 is abnormal and does not meet the freezing temperature of the stem cell supernatant, the controller will detect this abnormal temperature through the temperature sensor 5. Subsequently, the controller will control the indicator light 4 to flash, and remind the staff through the flashing of the indicator light 4 that the freezing temperature in the storage drawer 2 is abnormal and needs to be processed in time.

[0060] When the staff needs to take out the storage drawer 2, the staff can control the electromagnet 16 and the refrigerator 20 to turn off through the controller. After the refrigerator 20 is turned off, the freezing cover 13 will stop freezing the stem cell supernatant; after the electromagnet 16 is turned off, the electromagnet 16 will no longer generate an attractive force on the iron sheet 19. When the staff takes out the storage drawer 2, the second spring 17 will release the stored energy to provide a thrust for the removal of the storage drawer 2, helping the staff to take out the storage drawer 2 more labor - saving.

[0061] After the storage drawer 2 is taken out, the first spring 9 will also release the stored energy. The first spring 9 will push the second support column 12 to rotate. The rotation of the second support column 12 will drive the rotation of the connecting rod 8. When the connecting rod 8 rotates, it will drive the freezing cover 13 to rotate. The freezing cover 13 will slide backward along the first support platform 11 through the pulley 15, and finally the freezing cover 13 will return to the vertical position.

[0062] Embodiment 2:

[0063] The difference from the above - mentioned embodiment is that a sealing strip for reducing cold air leakage is adhesively bonded at the opening.

[0064] The specific implementation process is as follows: when the refrigerator 20 starts to work, there will be a large amount of cold air in the box body 1, and the sealing strip can effectively isolate the exchange of the outside air and the cold air in the box body 1, thereby improving the refrigeration efficiency of the refrigerator 20, and by isolating the exchange of the outside air and the cold air in the box body 1, the sealing strip is also beneficial to reduce the power consumption of the refrigerator 20, thereby better preserving the stem cell supernatant.

[0065] Embodiment 3:

[0066] As attached Figure 2 As shown, the difference from the above embodiment is that an alarm 3 is fixedly connected to the top of the box body 1 by screws, and the controller is used to control the alarm 3 to sound an alarm when the temperature changes abnormally.

[0067] The specific implementation process is as follows: when the temperature in the storage drawer 2 is abnormal, if the staff is not near the box 1, they cannot directly see the flashing of the indicator light 4, which may easily delay the processing time of the abnormal temperature in the storage drawer 2. At this time, the alarm 3 will immediately alarm and use voice prompts, such as "The temperature is abnormal, please deal with it immediately!" to remind the staff that they need to deal with the abnormal temperature in the storage drawer 2 immediately.

[0068] Embodiment 4:

[0069] The difference from the above embodiment is that the inner wall of the storage drawer 2 is covered with a heat-insulating layer, and the heat-insulating layer is preferably a polyurethane layer.

[0070] The specific implementation process is as follows: when the refrigerator 20 freezes the stem cell supernatant in the storage drawer 2 through the freezing cover 13, the insulation layer in the storage drawer 2 can reduce the heat exchange between the cold air in the storage drawer 2 and the external environment, reduce the leakage of cold air, and thus improve the freezing effect of the stem cell supernatant.

[0071] Embodiment 5:

[0072] As attached Figure 2 As shown, the difference from the above embodiment is that a partition plate 21 for separating the storage drawer 2 and the third support column 7 is fixedly connected to the inner bottom wall of the box body 1 by bolts.

[0073] The specific implementation process is as follows: during the process of taking out and pushing in the storage drawer 2, the partition plate 21 can effectively separate the third support column 7 from the storage drawer 2, reduce the shaking of the storage drawer 2 in the box body 1, and can reduce the collision between the storage drawer 2 and the third support column 7, and reduce the mutual influence between the storage drawer 2 and the third support column 7.

[0074] Obviously, the above embodiments are merely examples given for clear illustration and are not limitations on the implementation manners. For those of ordinary skill in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to exhaustively list all implementation manners here. And the obvious changes or modifications derived therefrom still fall within the protection scope of the present invention.

Claims

1. A cryopreservation device for stem cell supernatant, comprising a box body (1), and an opening for communicating with the outside is formed on one side of the box body (1), and it is characterized in that, On the inner side wall of the box body (1), a first support platform (11) and a second support platform (6) are fixedly connected, and the first support platform (11) is directly above the second support platform (6); On one side of the bottom end of the first support platform (11) away from the inner wall of the box body (1), a first support column (10) is fixedly connected, and a "へ”-shaped connecting rod (8) is sleeved and rotatably connected to the first support column (10); Both ends of the connecting rod (8) are fixedly connected with collar rings (14); a second support column (12) is fixedly connected inside the collar ring (14) near one end of the first support column (10); a freezing cover (13) is hinged on the collar ring (14) at the end away from the first support column (10); a pulley (15) is slidably fitted on the top of the first support platform (11), and the pulley (15) is rotatably connected to the freezing cover (13); A refrigeration component for refrigerating the stem cell supernatant is provided on the freezing cover (13); On one side of the second support platform (6) away from the inner wall of the box body (1), a third support column (7) is fixedly connected, and a first spring (9) is fixedly connected between the third support column (7) and the second support column (12); A storage component for storing the stem cell supernatant is provided in the opening; an attracting component for attracting the storage component is provided on the inner wall of the box body (1) at the end away from the opening.

2. The cryopreservation device for stem cell supernatant according to claim 1, wherein The storage component includes a storage drawer (2), and an iron sheet (19) and an extension plate (18) are fixedly connected to the outer wall of the storage drawer (2) on the side away from the opening of the box body (1), and the extension plate (18) is below the iron sheet (19); The attracting component includes an electromagnet (16), and the electromagnet (16) is fixedly connected to the inner wall of the box body (1); a controller is fixedly connected outside the box body (1), and the controller is used to control the start and stop of the electromagnet (16).

3. The cryopreservation device for stem cell supernatant according to claim 2, wherein A second spring (17) is fixedly connected between the electromagnet (16) and the iron sheet (19).

4. The stem cell supernatant cryopreservation device according to claim 3, wherein, The refrigeration component includes a refrigerator (20), and the refrigerator (20) is fixedly connected to the upper surface of the freezing cover (13), and the controller is used to control the start and stop of the refrigerator (20).

5. The cryopreservation device for stem cell supernatant according to claim 4, wherein A temperature sensor (5) is fixedly connected inside the storage drawer (2), and the controller is used to receive the temperature data of the temperature sensor (5).

6. The cryopreservation device for stem cell supernatant according to claim 5, characterized in that, A sealing strip for reducing cold air leakage is fixedly connected at the opening.

7. The cryopreservation device for stem cell supernatant according to claim 6, wherein An indicator light (4) is fixedly connected to the top of the box body (1), and the controller is used to control the indicator light (4) to flash to remind the staff when the temperature changes abnormally.

8. The cryopreservation device for stem cell supernatant according to claim 7, wherein, An alarm (3) is fixedly connected to the top of the box body (1), and the controller is used to control the alarm (3) to alarm when the temperature changes abnormally.

9. The cryopreservation device for stem cell supernatant according to claim 8, wherein, The inner wall of the storage drawer (2) is covered with a heat insulation layer.

10. The cryopreservation device for stem cell supernatant according to claim 9, wherein A partition board (21) for separating the storage drawer (2) and the third support column (7) is fixedly connected to the inner bottom wall of the box body (1).

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