Storage device and method for maintaining biomechanical strength of bone tissue

Abstract

The invention belongs to the technical field of bone tissue storage, and particularly relates to a storage device and method for keeping the biomechanical strength of bone tissue, and the storage device comprises a storage barrel used for placing the bone tissue; a sealing cover is detachably installed at the top of the storage tank, and a fixing pipe used for injecting liquid nitrogen is fixed to the outer wall of the storage tank; the supporting plate is fixed into the storage tank, an inner cavity of the storage tank is divided into a containing cavity and a liquid nitrogen cavity from top to bottom through the supporting plate, a first through groove is formed in the supporting plate, and cold air generated by liquid nitrogen evaporation is introduced into the containing cavity through the first through groove so as to conduct low-temperature storage on the storage barrel; by arranging the gas recovery assembly, cold air is recovered into the liquid nitrogen cavity before the storage barrel is taken out, and cold air leakage is effectively avoided in the process of taking out the storage barrel, so that resources are saved, and people are prevented from being hurt due to cold air leakage; the bone tissue is stored through the device and the method, and the biomechanical strength of the bone tissue can be kept to the maximum extent.

Description

Technical Field

[0001] The present invention belongs to the technical field of bone tissue storage, and specifically relates to a storage device and method for maintaining the biomechanical strength of bone tissue. Background Art

[0002] With the continuous development of the biomedical field, especially in tissue engineering, transplantation medicine, and cell therapy, the demand for preservation technologies for biological samples is increasing. The long-term preservation of bone tissue, bone marrow, and other tissues often requires the use of cryopreservation methods, and the liquid nitrogen preservation technology is widely used in the long-term storage of biological tissues and cells due to its superior low-temperature stability. Liquid nitrogen storage tanks have become common tools for storing biological samples such as bone tissue because they can provide a low-temperature environment of -196°C.

[0003] During the operation of a liquid nitrogen storage tank, when retrieving the stored samples, it is often necessary to open the lid or other closing devices of the storage tank, resulting in the evaporation of liquid nitrogen and the leakage of a large amount of cold air. Since liquid nitrogen rapidly vaporizes into nitrogen at room temperature, this process not only causes instability in the low-temperature environment but also may result in waste of liquid nitrogen resources. More seriously, if the operator operates improperly, they may suffer frostbite due to contact with the low-temperature liquid nitrogen cold air.

[0004] Therefore, the present invention provides a storage device and method for maintaining the biomechanical strength of bone tissue. Summary of the Invention

[0005] In order to make up for the deficiencies of the prior art and solve at least one of the technical problems proposed in the background art.

[0006] The technical solution adopted by the present invention to solve its technical problems is as follows: The storage device for maintaining the biomechanical strength of bone tissue according to the present invention includes: A storage barrel for placing bone tissue; A storage tank with a detachable sealing cover installed on the top, and a fixed tube for injecting liquid nitrogen fixed on the outer wall of the storage tank; A support plate fixed inside the storage tank, which divides the inner cavity of the storage tank into a placement cavity and a liquid nitrogen cavity from top to bottom through the support plate. The storage barrel is placed in the placement cavity, and liquid nitrogen is stored in the liquid nitrogen cavity. A first through groove is provided on the support plate, and the cold air generated by the evaporation of liquid nitrogen is introduced into the placement cavity through the first through groove to store the storage barrel at a low temperature; Gas recovery component, through which cold air is recovered into the liquid nitrogen chamber before the storage bucket is taken out. The gas recovery component includes a sealing ring that is hermetically and slidably installed inside the storage tank. The sealing ring is hermetically sleeved outside the storage bucket. A sleeve rod is fixed on the support plate. An active rod is slidably installed in the middle of the sleeve rod. The top end of the active rod is fixedly connected to the sealing ring. A lead screw is threadedly connected to the middle of the active rod. A motor for driving the rotation of the lead screw is fixed on the top of the storage tank.

[0007] Preferably, it further includes a lifting component for lifting the storage bucket. The lifting component includes a cylinder fixed in the middle of the liquid nitrogen chamber. A piston plate is hermetically and slidably installed inside the cylinder. A connecting rod is fixed on the top of the piston plate. A groove is opened at the center of the upper end surface of the support plate. A top plate is arranged in the groove. The connecting rod slidably penetrates through the through holes opened at the corresponding positions of the cylinder and the support plate and is fixed to the bottom of the top plate. A connecting pipe is fixed on the lower side wall of the cylinder. One end of the connecting pipe away from the cylinder communicates with the placement chamber.

[0008] Preferably, a blocking component is arranged on the support plate to make the first through groove in a conducting or closed state. The blocking component includes a movable block. A sliding cavity is horizontally opened in the middle of the first through groove. The movable block is slidably arranged in the sliding cavity. A first spring for resetting the movable block is fixed inside the sliding cavity. A magnetic block is fixed at the bottom end of the active rod. The magnetic block attracts the movable block to slide along the sliding cavity through magnetic force.

[0009] Preferably, a second through groove is opened in the middle of the movable block. A retaining ring is fixed in the middle of the second through groove. A flap is arranged in a fitting manner above the retaining ring. The flap is rotatably installed on the inner wall of the second through groove through a torsion spring.

[0010] Preferably, a pressure relief component is arranged in the lifting component. The pressure relief component includes an air outlet opened at the top of the cylinder. An installation cavity is opened inside the piston plate. Through holes are arranged on the upper and lower sides of the installation cavity. A movable plate is slidably installed in the middle of the installation cavity. A top rod is fixed on the top of the movable plate. The top rod extends above the piston plate through the through hole. A second spring is arranged below the movable plate to push the movable plate upward to block the upper through hole.

[0011] Preferably, a limiting ring is fixed on the inner wall of the upper part of the storage tank.

[0012] Preferably, a vertical rod is slidably installed in a vertical groove formed in the middle of the connecting rod, and a pushing block is slidably installed in a horizontal groove formed in the middle of the piston plate. The pushing block is formed by combining two right trapezoidal blocks. A third spring for driving the pushing block to move horizontally is installed inside the horizontal groove. The top end of the vertical rod extends above the top plate, and the bottom end of the vertical rod extends into the horizontal groove and abuts against the inclined surface of the left trapezoidal block in the pushing block. The movable plate is frustum-shaped, and the inclined surface of the right trapezoidal block of the pushing block is adapted to the inclined surface of the movable plate.

[0013] Preferably, a threaded hole is formed in the middle of the sealing cover, and a threaded rod is threadedly connected in the threaded hole. A pressing plate is rotatably installed at the bottom end of the threaded rod, and a turning handle is fixed at the top end of the threaded rod.

[0014] Preferably, a sealing ring is fixed on the lower end surface of the limiting ring and at the edge position of the central hole.

[0015] A method for storing the biomechanical strength of bone tissue, which uses the above-mentioned device for storing the biomechanical strength of bone tissue, includes the following steps: S1. Place the bone tissue into a storage barrel filled with a biological medium; S2. The motor drives the lead screw to rotate, pushing the movable rod to move downward along the sleeve rod, and then driving the sealing ring to move downward to squeeze the low-temperature gas below. The low-temperature gas inside the placement cavity is introduced into the cylinder through the connecting pipe, exhausting the low-temperature gas in the placement cavity, and pushing the piston plate, connecting rod and movable plate upward; S3. In step S2, during the downward movement of the movable rod, the driving magnet and the movable block are misaligned, and the movable block is pushed to move by the spring and the through groove is closed, so that the cold air enters the cylinder through the connecting pipe; S4. The sealing ring continues to move downward until it fits with the support plate. During this period, the excess cold air inside the cylinder is discharged into the liquid nitrogen cavity through the pressure relief component; S5. Open the sealing cover, insert the storage barrel along the central hole of the limiting ring until it contacts the top plate; S6. Control the motor to reverse, drive the lead screw, movable rod and sealing ring to move in the reverse direction, a negative pressure is generated inside the placement cavity, the low-temperature gas at the bottom of the piston plate is pumped into the placement cavity, and at the same time, the flap is driven to rotate upward to open, so that the low-temperature gas in the liquid nitrogen cavity enters the placement cavity through the through groove two in the middle of the movable block; S7. After the sealing plate moves up to the initial position, the movable plate moves down into the groove above the support plate, the storage barrel is completely received into the placement cavity, the sealing cover is installed, and finally the pressing plate is driven to press tightly on the top end of the storage barrel by rotating the threaded rod, and the storage work can be completed.

[0016] The beneficial effects of the present invention are as follows: 1. The storage device and method for maintaining the biomechanical strength of bone tissue according to the present invention recover cold air into the liquid nitrogen chamber through the gas recovery component before taking out the storage barrel. When it is necessary to take out the storage barrel, the motor drives the screw rod to rotate, thereby driving the movable rod to move downward along the sleeve rod, synchronously driving the sealing ring to move downward until it completely fits with the support plate. The sealing ring squeezes the cold air in the lower cavity, and all the cold air inside the placement cavity is recovered into the liquid nitrogen chamber. And through the tight fit of the sealing ring and the support plate, it prevents the cold air from flowing from the liquid nitrogen chamber to the placement cavity. In this way, during the process of opening the sealing cover and taking out the storage barrel, cold air leakage is effectively avoided, so as to save resources and avoid harm caused by the leakage of cold air. By storing bone tissue through this device and method, the biomechanical strength of bone tissue can be maximally maintained.

[0017] 2. The storage device and method for maintaining the biomechanical strength of bone tissue according to the present invention lift the storage barrel through the lifting component. When the sealing ring moves downward, it synchronously squeezes the cold air in the lower placement cavity. The cold air is introduced into the cylinder and accumulates below the piston plate, pushing the piston plate, connecting rod and top plate to move upward, and synchronously lifting the storage barrel upward, so that the top of the storage barrel protrudes from the top opening of the storage tank, facilitating the taking out of the storage barrel and improving the taking efficiency. Brief Description of the Drawings

[0018] The present invention will be further described below with reference to the accompanying drawings.

[0019] Figure 1 is a perspective view of the present invention; Figure 2 is the internal structure of the storage barrel of the present invention; Figure 3 is a partial structural cross-sectional view of the present invention; Figure 4 is a schematic structural view of the lifting component in the present invention; Figure 5 is Figure 4 the enlarged view at A in Figure 6 is a cross-sectional view of the plugging component in the present invention; Figure 7 is Figure 6 the enlarged view at B in Figure 8 is Figure 7 the enlarged view at C in Figure 9 is a flowchart of the method of the present invention.

[0020] In the figure: 1. Storage tank; 2. Sealing cover; 3. Fixed pipe; 4. Support plate; 5. Limiting ring; 6. Sealing ring; 7. Preservation barrel; 8. Placing cavity; 9. Liquid nitrogen cavity; 10. Lead screw; 11. Movable rod; 12. First through groove; 13. Sleeve rod; 14. Sealing ring; 15. Rotating handle; 16. Threaded rod; 17. Pressing plate; 18. Cylinder barrel; 19. Connecting rod; 20. Connecting pipe; 21. Piston plate; 22. Groove; 23. Top plate; 24. Movable plate; 25. Thrust rod; 26. First spring; 27. Air outlet hole; 28. Magnetic block; 29. Movable block; 30. Sliding cavity; 31. Second spring; 32. Stop ring; 33. Flap; 34. Second through groove; 35. Vertical rod; 36. Third spring; 37. Pushing block. Specific implementation manner

[0021] In order to make the technical means, creative features, achieved purposes and functions of the present invention easy to understand, the present invention will be further described below in conjunction with specific implementation manners.

[0022] Example 1: As Figures 1 to 8 shown, the storage device for maintaining the biomechanical strength of bone tissue according to the embodiment of the present invention includes: A preservation barrel 7 for placing bone tissue; A storage tank 1, a sealing cover 2 is detachably installed on the top of the storage tank 1, and a fixed pipe 3 for injecting liquid nitrogen is fixed on the outer wall of the storage tank 1; A support plate 4 is fixed inside the storage tank 1. Through the support plate 4, the inner cavity of the storage tank 1 is divided into a placing cavity 8 and a liquid nitrogen cavity 9 from top to bottom. The preservation barrel 7 is placed in the placing cavity 8, and liquid nitrogen is stored in the liquid nitrogen cavity 9. A first through groove 12 is opened on the support plate 4, and the cold air generated by the evaporation of liquid nitrogen is introduced into the placing cavity 8 through the first through groove 12 to store the preservation barrel 7 at a low temperature; A gas recovery component is used to recover the cold air into the liquid nitrogen cavity 9 before taking out the preservation barrel 7. The gas recovery component includes a sealing ring 6 that is hermetically slidably installed inside the storage tank 1. The sealing ring 6 is hermetically slidably sleeved outside the preservation barrel 7. A sleeve rod 13 is fixed on the support plate 4. A movable rod 11 is slidably installed in the middle of the sleeve rod 13. The top end of the movable rod 11 is fixedly connected to the sealing ring 6. A lead screw 10 is threadedly connected to the middle of the movable rod 11. A motor for driving the rotation of the lead screw 10 is fixed on the top of the storage tank 1; During operation, the storage barrel 7 is placed at the middle position on the upper end surface of the support plate 4. The sealing ring 6 is sleeved outside the storage barrel 7. A cavity for temporarily storing cold air is formed among the sealing ring 6, the storage tank 1, the support plate 4 and the storage barrel 7. The cold air generated by the evaporation of liquid nitrogen in the liquid nitrogen cavity 9 enters the cavity through the through groove to perform low-temperature preservation on the storage barrel 7 so as to maintain the activity of bone tissue. When it is necessary to take out the storage barrel 7, the gas recovery assembly is started. The lead screw 10 is rotated by the motor, and then the movable rod 11 is driven to move downward along the sleeve rod 13, and the sealing ring 6 is synchronously driven to move downward until it is completely attached to the support plate 4. The sealing ring 6 squeezes the cold air in the lower cavity, and all the cold air inside the placement cavity 8 is recovered into the liquid nitrogen cavity 9. And through the tight fit of the sealing ring 6 and the support plate 4, the cold air is prevented from flowing from the liquid nitrogen cavity 9 to the placement cavity 8. In this way, during the process of opening the sealing cover 2 to take out the storage barrel 7, cold air leakage is effectively avoided, so as to save resources and avoid cold air leakage from hurting people.

[0023] It further includes a lifting assembly. The storage barrel 7 is lifted by the lifting assembly. The lifting assembly includes a cylinder 18 fixed in the middle of the liquid nitrogen cavity 9. A piston plate 21 is hermetically and slidably installed inside the cylinder 18. A connecting rod 19 is fixed to the top of the piston plate 21. A groove 22 is formed in the center of the upper end surface of the support plate 4. A top plate 23 is arranged in the groove 22. The connecting rod 19 slidably penetrates through through holes opened at corresponding positions on the cylinder 18 and the support plate 4 and is fixed to the bottom of the top plate 23. A connecting pipe 20 is fixed to the lower side wall of the cylinder 18. One end of the connecting pipe 20 away from the cylinder 18 communicates with the placement cavity 8. During operation, when the sealing ring 6 moves downward, it synchronously squeezes the cold air in the lower placement cavity 8. The cold air is introduced into the inside of the cylinder 18 and accumulates below the piston plate 21. The volume of the placement cavity 8 is larger than the volume of the cylinder 18. Therefore, there is enough gas to push the piston plate 21, the connecting rod 19 and the top plate 23 to move upward, synchronously lifting the storage barrel 7 upward so that the top end of the storage barrel 7 protrudes from the top opening of the storage tank 1, facilitating the taking out of the storage barrel 7 and improving the taking efficiency.

[0024] A blocking assembly is arranged on the support plate 4. The through groove 12 is made to be in a conducting or closed state through the blocking assembly. The blocking assembly includes a movable block 29. A sliding cavity 30 is horizontally opened in the middle of the through groove 12. The movable block 29 is slidably arranged in the sliding cavity 30. A first spring 26 for resetting the movable block 29 is fixed inside the sliding cavity 30. A magnetic block 28 is fixed to the bottom end of the movable rod 11. The magnetic block 28 attracts the movable block 29 to slide along the sliding cavity 30 through magnetic force. During operation, when the storage barrel 7 is located inside the storage tank 1 for low-temperature storage, the movable rod 11 is at the topmost position. The magnet 28 at the bottom of the movable rod 11 is aligned with the movable block 29. The movable block 29 is attracted by the magnetic force to move horizontally along the sliding cavity 30, and the first spring 26 is compressed, so that the first through groove 12 is in a conducting state, allowing the cold air in the liquid nitrogen chamber 9 to enter the placement chamber 8 through the through groove and contact the side wall of the storage barrel 7 for low-temperature storage of the storage barrel 7. When the lead screw 10 drives the movable rod 11 to move downward, the lead screw 10 drives the magnet 28 to move downward, causing the magnet 28 to be misaligned with the movable block 29. At this time, the magnet 28 no longer attracts the movable block 29. Under the rebounding action of the first spring 26, the movable block 29 is pushed to reset, so that the movable block 29 closes the first through groove 12. At this time, the cold air in the placement chamber 8 cannot enter the liquid nitrogen chamber 9 through the first through groove 12, and the cold air can only be pressed into the cylinder barrel 18 through the connecting pipe 20 to drive the lifting assembly to move, realizing the lifting of the storage barrel 7 for easy removal of the storage barrel 7.

[0025] A second through groove 34 is formed in the middle of the movable block 29. A retaining ring 32 is fixed in the middle of the second through groove 34. A flap 33 is disposed in a fitting manner above the retaining ring 32. The flap 33 is rotatably mounted on the inner wall of the second through groove 34 through a torsion spring. During operation, when the placement control motor rotates in reverse, driving the lead screw 10, the movable rod 11 and the sealing ring 6 to move in the reverse direction, a negative pressure is generated inside the placement chamber 8, driving the flap 33 to rotate upward and open, so that the cold air in the liquid nitrogen chamber 9 enters the placement chamber 8 through the second through groove 34 in the middle of the movable block 29 for low-temperature storage of the storage barrel 7.

[0026] A pressure relief component is provided in the lifting component. The pressure relief component includes an air outlet hole 27 formed in the top of the cylinder barrel 18. An installation cavity is formed inside the piston plate 21. Through holes are provided on the upper and lower sides of the installation cavity. A movable plate 24 is slidably mounted in the middle of the installation cavity. A top rod 25 is fixed to the top of the movable plate 24. The diameter of the top rod 25 is smaller than the inner diameter of the through hole above the installation cavity. The top rod 25 extends above the piston plate 21 through the through hole. A second spring 31 is disposed below the movable plate 24 to push the movable plate 24 upward to block the upper through hole. During operation, the cold air in the placement chamber 8 is pressed into the bottom of the piston plate 21, pushing the piston plate 21 upward until the cushion block at the top of the piston plate 21 contacts the inner wall of the top of the cylinder barrel 18. During this process, the top rod 25 first contacts the top of the cylinder barrel 18 and is squeezed and contracted into the installation cavity. At this time, the movable plate 24 is separated from the top end of the installation cavity, and the cold air under the piston plate 21 can enter the upper space through the installation cavity and be discharged into the liquid nitrogen chamber 9 through the air outlet hole 27. This can reduce the pressure inside the cylinder barrel 18 and prevent the cylinder barrel 18 from being damaged due to excessive pressure inside the cylinder barrel 18. As the cold air in the placement chamber 8 is gradually discharged into the liquid nitrogen chamber 9, the internal pressure of the cylinder barrel 18 tends to be stable. At this time, the movable plate 24 will gradually move upward under the action of the second spring 31 and seal the top through hole of the installation chamber, preventing the gas below the piston plate 21 from leaking out continuously, so that the piston plate 21, the connecting rod 19 and the top plate 23 can stably support the storage barrel 7. In addition, a vertical notch is provided on the side of the movable plate 24. The notch is arranged at the edge position of the movable plate 24. When the movable plate 24 is attached to the inner wall of the top of the installation chamber, there is no air leakage in the notch. When the movable plate 24 moves downward, the gas flow rate is increased through the notch, which is convenient for accelerating the discharge of the redundant gas.

[0027] A limiting ring 5 is fixedly installed on the upper inner wall of the storage tank 1; during operation, the size of the middle hole of the limiting ring 5 is adapted to the outer diameter of the storage barrel 7. When placing the storage barrel 7, the storage barrel 7 is lowered from the middle hole of the limiting ring 5, and the limiting ring 5 limits the storage barrel 7 in the horizontal direction to prevent horizontal movement during the transportation process; in addition, the outer wall of the storage barrel 7 is attached to the inner wall of the middle hole of the limiting ring 5. During the process of the storage barrel 7 being lowered through the middle hole, there is friction between the storage barrel 7 and the inner wall of the middle hole of the limiting ring 5, so that the storage barrel 7 is slowly lowered to avoid damage to the storage barrel 7.

[0028] A vertical rod 35 is slidably installed in a vertical groove formed in the middle of the connecting rod 19, and a push block 37 is slidably installed in a horizontal groove formed in the middle of the piston plate 21. The push block 37 is formed by combining two right trapezoidal blocks. A third spring 36 for driving the push block 37 to move horizontally is installed inside the horizontal groove. The top end of the vertical rod 35 extends above the top plate 23, and the bottom end of the vertical rod 35 extends into the horizontal groove and abuts against the inclined surface of the left trapezoidal block in the push block 37. The movable plate 24 is frustum-shaped, and the inclined surface of the right trapezoidal block in the push block 37 is adapted to the inclined surface of the movable plate 24. During operation, the storage barrel 7 is lifted up by the lifting assembly, and then the storage barrel 7 is taken out. At this time, the restriction on the top of the vertical rod 35 is removed, and the push block 37 is driven to move horizontally by the rebound of the spring three 36, and the vertical rod 35 is pushed upward by the inclined surface of the left trapezoidal block, and the end of the trapezoidal block on the right side of the push block 37 squeezes the upper inclined surface of the movable plate 24, driving the movable plate 24 to move downward. At this time, the installation cavity is connected, and the piston plate 21, the connecting rod 19 and the pressure plate 17 are affected by gravity and automatically descend, thereby discharging the gas under the piston plate 21 until the pressure plate 17 moves down to the groove 22 at the top of the support plate 4; When a new preservation barrel 7 is placed later, the preservation barrel 7 can move down along the middle hole of the limiting ring 5 until the bottom contacts the top of the support plate 4. When the sealing ring 6 is driven to move upward, the sealing ring 6 squeezes the air above and discharges the air from the through hole opened on the limiting ring 5, thereby reducing the pressure between the limiting ring 5 and the sealing ring 6. At the same time, the cold air in the liquid nitrogen chamber 9 is discharged upward into the cavity below the sealing ring 6 through the middle through groove 2 34 of the movable block 29. During the movement of the sealing ring 6, it always fits with the outer wall of the preservation barrel 7, thereby effectively preventing the leakage of cold air. The cold air contacts the side wall of the preservation barrel 7, thereby preserving the preservation barrel 7 at a low temperature. In addition, the storage barrel 7 can press down the vertical rod 35, and the bottom end of the vertical rod 35 contacts the inclined surface of the trapezoidal block on the left side of the push block 37, thereby driving the push block 37 to separate from the movable plate 24, and driving the movable plate 24 to return to the through hole at the top of the closed installation cavity through the spring 2 31, so as to make advance preparations for the next lifting work.

[0029] A threaded hole is provided in the middle of the sealing cover 2, in which a threaded rod 16 is threadedly connected, a pressure plate 17 is rotatably installed at the bottom end of the threaded rod 16, and a turning handle 15 is fixed to the top end of the threaded rod 16; when working, the preservation barrel 7 is first lowered from the middle hole of the limiting ring 5, and then the sealing cover 2 is tightened in the open top of the storage tank 1, and then the threaded rod 16 is rotated by the turning handle 15 to drive the pressure plate 17 to move downward and press on the top of the preservation barrel 7, so as to limit the preservation barrel 7 in the vertical direction, so that the preservation barrel 7 can be stably stored inside the storage tank 1.

[0030] Embodiment 2: Figures 1 to 4 As shown, in contrast to Example 1, another implementation of the present invention is: a sealing ring 14 is fixed on the lower end surface of the limiting ring 5 and located at the edge of the center hole, and the sealing ring 14 is a hollow structure and is made of elastic material; during operation, when the sealing ring 6 moves toward the limiting ring 5, the sealing ring 14 is squeezed by the sealing ring 6 and the limiting ring 5, and the sealing ring 14 is deformed and fits precisely with the outer wall of the preservation barrel 7, thereby further preventing cold air leakage.

[0031] like Figure 9 As shown, a method for storing the biomechanical strength of bone tissue is provided, wherein the method adopts the above-mentioned storage device for storing the biomechanical strength of bone tissue, and comprises the following steps: S1. Place the bone tissue into the storage barrel 7 filled with biological medium; S2. The motor drives the screw rod 10 to rotate, pushing the movable rod 11 to move downward along the sleeve rod 13, and then driving the sealing ring 6 to move downward to squeeze the low-temperature gas below. The low-temperature gas inside the placement cavity 8 is introduced into the cylinder barrel 18 through the connecting pipe 20, evacuating the low-temperature gas in the placement cavity 8, and pushing the piston plate 21, the connecting rod 19, and the movable plate 24 upward; S3. In step S2, during the downward movement of the movable rod 11, the driving magnet block 28 is misaligned with the movable block 29, and the movable block 29 is pushed to move by the spring to close the through groove, so that the cold air enters the cylinder barrel 18 through the connecting pipe 20; S4. The sealing ring 6 continues to move downward until it fits against the support plate 4. During this period, the excess cold air inside the cylinder barrel 18 is discharged into the liquid nitrogen cavity 9 through the pressure relief component; S5. Open the sealing cover 2, insert the storage barrel 7 along the central hole of the limiting ring 5 until it contacts the top plate 23; S6. Control the motor to reverse, driving the screw rod 10, the movable rod 11, and the sealing ring 6 to move in the reverse direction. A negative pressure is generated inside the placement cavity 8, sucking the low-temperature gas at the bottom of the piston plate 21 into the placement cavity 8. At the same time, drive the flap 33 to rotate upward to open, so that the low-temperature gas in the liquid nitrogen cavity 9 enters the placement cavity 8 through the through groove two 34 in the middle of the movable block 29; S7. After the sealing plate moves upward to the initial position, the movable plate 24 moves downward into the groove 22 above the support plate 4, and the storage barrel 7 is completely received into the placement cavity 8. Install the sealing cover 2, and finally drive the pressing plate 17 to press tightly on the top of the storage barrel 7 by rotating the threaded rod 16, then the storage work can be completed.

[0032] The above front, back, left, right, up, and down are all based on the Figure 1 description in the accompanying drawings of the specification. Taking the perspective of the person observing as the standard, the side of the device facing the observer is defined as the front, the left side of the observer is defined as the left, and so on.

[0033] In the description of the present invention, it should be understood that the orientation or positional relationship indicated by the terms "center", "longitudinal", "transverse", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the accompanying drawings. It is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as limiting the protection scope of the present invention.

[0034] The foregoing has shown and described the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited by the above embodiments, and what is described in the above embodiments and the specification is only to illustrate the principle of the present invention. Without departing from the spirit and scope of the present invention, the present invention will have various changes and improvements, and these changes and improvements fall within the scope of the present invention claimed. The scope of protection claimed by the present invention is defined by the appended claims and their equivalents.

Claims

1. Bone tissue biomechanical strength storage device, characterized in that: Comprising: A storage barrel (7) for placing bone tissue; A storage tank (1) with a sealing cover (2) detachably installed on the top thereof, and a fixing pipe (3) for injecting liquid nitrogen is fixed on the outer wall of the storage tank (1); A support plate (4) fixed inside the storage tank (1). The inner cavity of the storage tank (1) is divided into a placement cavity (8) and a liquid nitrogen cavity (9) from top to bottom by the support plate (4). The storage barrel (7) is placed in the placement cavity (8), and liquid nitrogen is stored in the liquid nitrogen cavity (9). A first through groove (12) is formed on the support plate (4), and cold air generated by the evaporation of liquid nitrogen is introduced into the placement cavity (8) through the first through groove (12) to store the storage barrel (7) at a low temperature; A gas recovery assembly for recovering the cold air into the liquid nitrogen cavity (9) before taking out the storage barrel (7). The gas recovery assembly includes a sealing ring (6) slidably installed inside the storage tank (1) in a sealed manner. The sealing ring (6) is sleeved outside the storage barrel (7) in a sealed and slidable manner. A sleeve rod (13) is fixed on the support plate (4). A movable rod (11) is slidably installed in the middle of the sleeve rod (13). The top end of the movable rod (11) is fixed to the sealing ring (6). A lead screw (10) is threadedly connected to the middle of the movable rod (11). A motor for driving the rotation of the lead screw (10) is fixed on the top of the storage tank (1).

2. The bone tissue biomechanical strength maintaining storage device according to claim 1, characterized in that: It further includes a lifting assembly for lifting the storage barrel (7). The lifting assembly includes a cylinder (18) fixed in the middle of the liquid nitrogen cavity (9). A piston plate (21) is slidably installed inside the cylinder (18) in a sealed manner. A connecting rod (19) is fixed to the top of the piston plate (21). A groove (22) is formed in the center of the upper end surface of the support plate (4). A top plate (23) is arranged in the groove (22). The connecting rod (19) slidably penetrates through through holes formed at corresponding positions on the cylinder (18) and the support plate (4) and is fixed to the bottom of the top plate (23). A connecting pipe (20) is fixed to the lower side wall of the cylinder (18). One end of the connecting pipe (20) away from the cylinder (18) communicates with the placement cavity (8).

3. The bone tissue biomechanical strength maintaining storage device according to claim 2, wherein: A blocking assembly is arranged on the support plate (4) to make the first through groove (12) in a conducting or closed state. The blocking assembly includes a movable block (29). A sliding cavity (30) is horizontally formed in the middle of the first through groove (12). The movable block (29) is slidably arranged in the sliding cavity (30). A first spring (26) for resetting the movable block (29) is fixed inside the sliding cavity (30). A magnetic block (28) is fixed to the bottom end of the movable rod (11). The magnetic block (28) attracts the movable block (29) to slide along the sliding cavity (30) through magnetic force.

4. The bone tissue biomechanical strength maintaining storage device according to claim 3, characterized in that: A second through groove (34) is formed in the middle of the movable block (29). A retaining ring (32) is fixed in the middle of the second through groove (34). A flap (33) is arranged in a fitting manner above the retaining ring (32). The flap (33) is rotatably installed on the inner wall of the second through groove (34) through a torsion spring.

5. The bone tissue biomechanical strength preservation storage device according to claim 4, characterized in that: A pressure relief component is arranged in the lifting component. The pressure relief component includes an air outlet hole (27) opened at the top of the cylinder barrel (18). An installation cavity is opened inside the piston plate (21). Through holes are arranged on the upper and lower sides of the installation cavity. A movable plate (24) is slidably installed in the middle of the installation cavity. A top rod (25) is fixed to the top of the movable plate (24). The top rod (25) extends above the piston plate (21) through the through hole. A second spring (31) is arranged below the movable plate (24). The second spring (31) pushes the movable plate (24) to move upward to block the upper through hole.

6. The bone tissue biomechanical strength maintaining storage device according to claim 5, characterized in that: A limiting ring (5) is fixed to the inner wall of the upper part of the storage tank (1).

7. The bone tissue biomechanical strength maintaining storage device according to claim 6, characterized in that: A vertical rod (35) is slidably installed in a vertical groove opened in the middle of the connecting rod (19). A pushing block (37) is slidably installed in a horizontal groove opened in the middle of the piston plate (21). The pushing block (37) is formed by combining two right trapezoidal blocks. A third spring (36) for driving the pushing block (37) to move horizontally is installed inside the horizontal groove. The top end of the vertical rod (35) extends above the top plate (23). The bottom end of the vertical rod (35) extends into the horizontal groove and abuts against the inclined surface of the left trapezoidal block in the pushing block (37). The movable plate (24) is in a frustum shape. The inclined surface of the right trapezoidal block of the pushing block (37) is adapted to the inclined surface of the movable plate (24).

8. The bone tissue biomechanical strength maintaining storage device according to claim 7, characterized in that: A threaded hole is opened in the middle of the sealing cover (2). A threaded rod (16) is threadedly connected in the threaded hole. A pressing plate (17) is rotatably installed at the bottom end of the threaded rod (16). A turning handle (15) is fixed to the top end of the threaded rod (16).

9. The bone tissue biomechanical strength maintaining storage device according to claim 8, wherein: A sealing ring (14) is fixed to the lower end surface of the limiting ring (5) and at the edge position of the central hole.

10. A method for storing the biomechanical strength of bone tissue, which uses the device for storing the biomechanical strength of bone tissue according to claim 9, characterized in that: It includes the following steps: S1. Put the bone tissue into the preservation barrel (7) filled with biological medium; S2. The motor drives the lead screw (10) to rotate, pushes the movable rod (11) to move downward along the sleeve rod (13), and then drives the sealing ring (6) to move downward to squeeze the lower low-temperature gas. The low-temperature gas inside the placement cavity (8) is introduced into the cylinder barrel (18) through the connecting pipe (20), the low-temperature gas in the placement cavity (8) is emptied, and the piston plate (21), the connecting rod (19) and the movable plate (24) are pushed to move upward; S3. In step S2, during the downward movement of the movable rod (11), the driving magnetic block (28) is displaced from the movable block (29), and the movable block (29) is pushed to move by the spring and the through groove is closed, so that the cold air enters the cylinder barrel (18) through the connecting pipe (20); S4. The sealing ring (6) continuously moves downward until it fits with the support plate (4). During this period, the excess cold air inside the cylinder barrel (18) is discharged into the liquid nitrogen cavity (9) through the pressure relief component; S5. Open the sealing cover (2), insert the preservation barrel (7) into the central hole of the limiting ring (5) until it contacts the top plate (23); S6. Reverse the control of the motor to drive the lead screw (10), the movable rod (11) and the sealing ring (6) to move in the reverse direction, generating negative pressure inside the placement cavity (8), sucking the low-temperature gas at the bottom of the piston plate (21) into the placement cavity (8), and at the same time driving the flap (33) to rotate upward to open, so that the low-temperature gas in the liquid nitrogen cavity (9) enters the placement cavity (8) through the second through groove (34) in the middle of the movable block (29); S7. After the sealing plate moves up to the initial position, the movable plate (24) moves down into the groove (22) above the support plate (4), the storage barrel (7) is completely received into the placement cavity (8), the sealing cover (2) is installed, and finally the pressure plate (17) is driven to press against the top of the storage barrel (7) by rotating the threaded rod (16), and the storage work can be completed.

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