Aphid antifeedant composition and application thereof

Abstract

The invention relates to an aphid antifeedant composition and application thereof, the antifeedant composition comprises abietic acid and borneol, and based on the antifeedant composition, green control of aphids can be realized.

Description

Technical Field

[0001] The present invention relates to the field of pest control, and particularly to an aphid antifeedant and its application. Background Art

[0002] Aphids are an important class of phytophagous pests. While sucking the sap of plants, they also spread various plant viruses. The overuse of traditional chemical pesticides has caused major problems such as pest resistance and environmental pollution. Therefore, it is urgent to develop efficient plant-derived green pesticides to control aphids. Summary of the Invention

[0003] One aspect of the present invention provides an antifeedant composition that causes aphid antifeeding behavior, which comprises abietic acid and borneol.

[0004] In a specific embodiment, the mass ratio of abietic acid to borneol is from 1:1 to 1:2.

[0005] In a specific embodiment, the antifeedant composition further comprises a pharmaceutically acceptable carrier.

[0006] In a specific embodiment, the pharmaceutically acceptable carrier is an aqueous ethanol solution of 50 wt%.

[0007] In a specific embodiment, the aphid is Acyrthosiphon pisum.

[0008] Another aspect of the present invention provides the application of the antifeedant composition according to any one of the above in controlling aphids.

[0009] In a specific embodiment, the aphid is Acyrthosiphon pisum.

[0010] The term "insect antifeeding behavior" refers to the behavior of insects showing avoidance of feeding when facing certain plants or environments. For example, "aphid antifeeding behavior" refers to the behavior of aphids showing avoidance of feeding when facing certain plants or environments.

[0011] Advantages of the present invention: The present invention discovers that although abietic acid and borneol alone can hardly cause aphid antifeeding behavior, when the two are mixed in a mass ratio of 1:1 to 1:2, they can cause aphid antifeeding behavior. Based on this, green control of aphids can be achieved. In addition, the compounds abietic acid and borneol involved in the present invention are both natural components from plants, which are safe and friendly to the environment and can effectively reduce environmental pollution problems. And it achieves pest control by interfering with the feeding behavior of insects, has very little impact on non-target organisms, further reflecting its ecological friendliness and potential for sustainable development. Brief Description of the Drawings

[0012] Figure 1The antifeedant index of pea aphids treated with different compounds. The same letters in a, b, c, d, and e indicate no significant difference at the α=0.05 level, and different letters indicate significant difference at the α=0.05 level. Solvent represents solvent (50% ethanol aqueous solution), Abietic acid represents rosin acid, and Borneol represents borneol. 1:1, 1:2, 1:3, 2:1, and 3:1 represent the mass ratios of rosin acid and borneol, respectively. DETAILED DESCRIPTION

[0013] The present invention is further described below by specific examples. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. Unless otherwise specified, the reagents in the examples of the present invention are all commercially available.

[0014] The abietic acid and borneol used in the present invention can be purchased commercially. Example 1

[0015] Preparation of single-dose solution: dissolve rosin acid (molecular weight 302.45) in 50wt% ethanol aqueous solution to prepare a rosin acid solution with a concentration of 1 mg / mL; dissolve borneol (molecular weight 154.25) in 50wt% ethanol aqueous solution to prepare a borneol solution with a concentration of 1 mg / mL.

[0016] Preparation of mixed solution: The rosin acid solution and the borneol solution were fully mixed in a mass ratio of 1:1, 1:2, 1:3, 2:1 and 3:1 to form mixed solutions with different ratios.

[0017] A 50 wt % ethanol aqueous solution was used as a blank control.

[0018] Antifeedant effect of abietic acid on Acyrthosiphon pisum: Four round holes with a diameter of 1.0 cm were evenly punched in the bottom of a petri dish with a diameter less than 9 cm, and the centers of the four round holes were connected in sequence to form a square, which was called the leaf cover plate; 25 mL of 16 g / L agar matrix was placed in the bottom of a 9-cm-diameter petri dish for leaf moisturization; broad bean leaves were made into round leaves with a diameter of 1.5 cm using a hole puncher, and the backs of the four round leaves were placed upwards on the agar matrix, and the centers of the four round leaves were connected in sequence to form a square. The leaf cover plate was covered on the agar matrix with the leaves, and the four round leaves were exposed from the four round holes of the cover plate to form a leaf disc. 10 μL of 50 wt% ethanol aqueous solution was coated on two diagonal leaves with a small brush, and 10 μL of abietic acid solution with the same concentration was coated on the other two diagonal leaves with a small brush to exclude the influence of factors such as orientation in the environment. At the 6th h after the start of the experiment, the feeding A. pisum were counted and the antifeedant rate was calculated. 20 effective insects were taken as one replicate, and at least 8 biological replicates were carried out. One-way analysis of variance was used for significance analysis. Antifeedant index (AI) = (number of feeding insects on blank control leaves - number of feeding insects on treated leaves) / (number of feeding insects on blank control leaves + number of feeding insects on treated leaves).

[0019] Antifeedant effect of borneol on Acyrthosiphon pisum: Replace abietic acid in the above abietic acid experiment with borneol, and the other operations are the same.

[0020] Antifeedant effect of the mixture of abietic acid and borneol with a mass ratio of 1:1 on Acyrthosiphon pisum: Replace abietic acid in the above abietic acid experiment with a mixture of abietic acid and borneol with a mass ratio of 1:1, and the other operations are the same.

[0021] Antifeedant effect of the mixture of abietic acid and borneol with a mass ratio of 1:2 on Acyrthosiphon pisum: Replace abietic acid in the above abietic acid experiment with a mixture of abietic acid and borneol with a mass ratio of 1:2, and the other operations are the same.

[0022] Antifeedant effect of the mixture of abietic acid and borneol with a mass ratio of 1:3 on Acyrthosiphon pisum: Replace abietic acid in the above abietic acid experiment with a mixture of abietic acid and borneol with a mass ratio of 1:3, and the other operations are the same.

[0023] Antifeedant effect of the mixture of abietic acid and borneol with a mass ratio of 2:1 on Acyrthosiphon pisum: Replace abietic acid in the above abietic acid experiment with a mixture of abietic acid and borneol with a mass ratio of 2:1, and the other operations are the same.

[0024] Antifeedant effect of the mixture of abietic acid and borneol with a mass ratio of 3:1 on Acyrthosiphon pisum: Replace abietic acid in the above abietic acid experiment with a mixture of abietic acid and borneol with a mass ratio of 3:1, and the other operations are the same.

[0025] The antifeedant results of Acyrthosiphon pisum are shown in Figure 1 . As shown by Figure 1 , there were no significant differences between abietic acid and borneol alone and the blank control, indicating that abietic acid and borneol alone could hardly cause antifeeding in Acyrthosiphon pisum. When the two components were mixed, there were also no significant differences between the mixtures with the mass ratios of abietic acid to borneol of 2:1 and 3:1 and the blank control, indicating that the mixtures with these two ratios could hardly cause antifeeding in Acyrthosiphon pisum. However, when they were mixed at the mass ratios of 1:1 and 1:2, the antifeeding indices of both were significantly higher than those of the blank control, abietic acid and borneol alone, indicating that the two compounds had a synergistic effect at this time, producing unexpected technical effects.

Claims

1. An antifeedant composition that causes aphids to exhibit antifeedant behavior, which comprises abietic acid and borneol.

2. The feeding deterrent composition according to claim 1, characterized in that, The mass ratio of the abietic acid to the borneol is 1:1 to 1:

2.

3. The anorectic composition according to claim 1, wherein The antifeedant composition further comprises a pharmaceutically acceptable carrier.

4. The antifeedant composition according to claim 3, characterized in that, The pharmaceutically acceptable carrier is an aqueous ethanol solution of 50 wt%.

5. The antifeedant composition according to any one of claims 1 to 4, characterized in that, The aphids are Acyrthosiphon pisum.

6. Use of the antifeedant composition according to any one of claims 1 to 5 in controlling aphids.

7. The application according to claim 6, wherein The aphids are Acyrthosiphon pisum.

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