Bactericidal composition and application thereof

Abstract

The invention belongs to the technical field of pesticide sterilization, and discloses a sterilization composition and application thereof.The sterilization composition comprises an active ingredient A and an active ingredient B. The active ingredient A is Cyclobutrifluram, and the active ingredient B is any one of ipconazole, metconazole or triticonazole. The mass ratio of the active component A to the active component B is (1: 50)-(55: 1). The bactericidal composition disclosed by the invention has a very good prevention and treatment effect on diseases caused by fusarium fungi, has a remarkable synergistic effect, reduces the use frequency and the use dosage of pesticides, reduces the production cost and reduces the environmental pollution.

Description

[0001] This divisional application of the present invention has an application number of 202311057228.X, a filing date of August 22, 2023, and an invention title of "A Bactericidal Composition and Its Application". Technical Field

[0002] The present invention belongs to the field of pesticide compounding, and specifically relates to a bactericidal composition and its application. Background Art

[0003] Triazole fungicides are a class of highly effective fungicides developed in the 1970s. They exert their efficacy by inhibiting the biosynthesis of ergosterol in the fungal cells, have good systemic properties, and are highly effective, low-toxic, have a long-lasting effect, and have both preventive and therapeutic effects. They can be used to control a variety of diseases caused by ascomycetes, basidiomycetes, and deuteromycetes.

[0004] Cyclobutrifluram is a novel nicotinamide bactericidal and nematicidal agent developed by Syngenta. IUPAC name: N-[2-(2,4-dichlorophenyl)cyclobutyl]-2-(trifluoromethyl)nicotinamide, which contains 80%-100% of the (1S,2S)-enantiomer and 0-20% of the (1R,2R)-enantiomer; CAS registry number: 1460292-16-3; molecular formula: C 17 H 13 CI2F3N2O. Cyclobutrifluram has a broad control spectrum, is highly effective, has a low dosage, is easy to use, can effectively control various nematodes and major fungal diseases, can be used for soil treatment or seed treatment, and can provide long-term control of nematodes and diseases in major crops and various environments, especially having excellent control effects against Fusarium.

[0005] Fusarium fungi are important pathogenic bacteria that cause various soil-borne diseases in crops. In agricultural production, they can cause a variety of plant diseases, such as wheat head blight, wheat basal stalk rot, rice bakanae disease, etc. Serious occurrence of these diseases causes significant economic losses to agricultural production.

[0006] Wheat basal stalk rot is a basal stalk disease caused by various Fusarium spp., which is a worldwide soil-borne disease. It is mainly manifested as brown rot at the basal stalk, onset of the disease, browning of the wheat coleoptile and base. During the filling stage of wheat, it causes the tillering part at the basal stalk to wither, and the upper part dies. The stem leaves and ears cannot obtain water and die, showing a withered white ear. When pulled out in the field, it is extremely easy to break from the base. The plants with mild disease grow thin and weak and soon wither, which has a very great impact on the yield. Wheat basal stalk rot has the characteristics of strong concealment and difficulty in detection at the seedling stage. By the time the symptoms appear above the ground, the best control period has been missed. If not controlled in time, it will cause a significant reduction in production or even a complete crop failure, commonly known as "the cancer of wheat". Research has found that there is no highly resistant variety to wheat basal stalk rot among the main popularized varieties in production, and crop rotation is difficult to implement. There is a lack of particularly effective control methods for the prevention and control of basal stalk rot.

[0007] Bakanae disease of rice is a seed-borne disease of rice caused by Fusarium, also known as foolish seedling disease, white stalk disease, etc. Generally, the disease can cause a 5% - 20% reduction in rice yield, and in severe cases, more than 50% reduction. After being infected by the bakanae pathogen of rice, the diseased plants show foolish growth at the 2 - 4 leaf stage. The plants are taller than the healthy plants, with long and narrow leaves and leaf sheaths, and abnormal root development, growing thin and weak. Diseased plants generally cannot panicle or cannot fully panicle. Even if they panicle, the panicles are small and unfilled. In recent years, bakanae disease of rice has shown an upward trend in the main rice-producing areas of our country. It has occurred severely in some areas, posing a threat to the stable and high yield of rice.

[0008] By measuring the bioactivity of compounds against target organisms and different variety combinations, it is one of the effective methods to develop pesticides, improve the control effect and delay drug resistance. The applicant has conducted in-depth research on Cyclobutrifluram combined with prothioconazole, metconazole or difenoconazole and any combination of Cyclobutrifluram with prothioconazole, metconazole or difenoconazole. It has been found that when Cyclobutrifluram is mixed with any one of prothioconazole, metconazole or difenoconazole, there is an obvious synergistic effect on Fusarium within a certain mixing ratio range, which can effectively improve the control of soil-borne diseases caused by Fusarium. Through further research, the present invention has been completed. There is no report on the mixing of Cyclobutrifluram with any one of prothioconazole, metconazole or difenoconazole for the control of diseases caused by Fusarium fungi. Summary of the Invention

[0009] Based on the above problems, the present invention provides a bactericidal composition, which can effectively control the diseases caused by Fusarium fungi, has an obvious synergistic effect, can effectively reduce the dosage of pesticides, and is environmentally friendly.

[0010] To achieve the above object, the present invention adopts the following technical solution: a bactericidal composition, the bactericidal composition comprising active ingredient A and active ingredient B, the active ingredient A being Cyclobutrifluram, and the active ingredient B being any one of prothioconazole, metconazole or triadimefon, and the mass ratio of the active ingredient A to the active ingredient B being 1:50 to 55:1;

[0011] Further, the active ingredient B is prothioconazole, and the mass ratio of the active ingredient A to the active ingredient B is 1:50 to 48:1;

[0012] Even further, the active ingredient B is prothioconazole, and the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 48:1;

[0013] Further, the active ingredient B is metconazole, and the mass ratio of the active ingredient A to the active ingredient B is 1:40 to 55:1;

[0014] Even further, the active ingredient B is metconazole, and the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 48:1;

[0015] Further, the active ingredient B is triadimefon, and the mass ratio of the active ingredient A to the active ingredient B is 1:32 to 48:1;

[0016] Even further, the active ingredient B is triadimefon, and the mass ratio of the active ingredient A to the active ingredient B is 1:25 to 30:1;

[0017] Further, based on 100 wt% of the total weight of the bactericidal combination, the total weight of the active ingredient A and the active ingredient B accounts for 0.1% to 80% of the total weight of the bactericidal composition;

[0018] Further, the bactericidal composition further comprises agriculturally acceptable auxiliary ingredients in addition to the active ingredients, and the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreezing agents, defoaming agents, solvents, preservatives, stabilizers, synergists, binders, fillers or carriers;

[0019] Further, the wetting agent is selected from one or more of alkylbenzene sulfonates, alkylnaphthalene sulfonates, lignin sulfonates, sodium dodecyl sulfate, sodium dioctyl sulfosuccinate, α-olefin sulfonates, alkylphenol polyoxyethylene ethers, castor oil polyoxyethylene ethers, alkylphenol ethoxylates, fatty alcohol ethoxylates, fatty alcohol polyoxyethylene ether sulfates, silkworm excrement, Chinese honey locust powder, sapindus powder, SOPA, detergents, emulsifier 2000 series and wetting penetrant F; and / or

[0020] The dispersant is selected from one or more of lignosulfonates, alkylnaphthalenesulfonate formaldehyde condensates, naphthalenesulfonates, triphenylvinylphenol ethoxylate phosphates, fatty alcohol ethoxylates, alkylphenol polyoxyethylene ethers, alkylphenol polyoxyethylene ether methyl ether condensate sulfates, fatty amine polyoxyethylene ethers, glycerol fatty acid ester polyoxyethylene ethers, polycarboxylates, polyacrylic acids, phosphates, EO-PO block copolymers and EO-PO graft copolymers; and / or

[0021] The emulsifier is selected from one or more of calcium dodecylbenzenesulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether and alkylphenol ether phosphate; and / or

[0022] The thickener is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and silica white; and / or

[0023] The disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and / or

[0024] The antifreeze is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons and inorganic salts; and / or

[0025] The defoamer is selected from one or more of C 10 -C 20 saturated fatty acid compounds, silicone oils, silicone compounds, C8-C 10 fatty alcohols; and / or

[0026] The solvent is selected from one or more of benzene, toluene, xylene, durene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel oil, solvent naphtha, vegetable oil, vegetable oil derivatives and water; and / or

[0027] The preservative is selected from one or more of propionic acid, sodium propionate, sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate, Kathon and 1,2-benzisothiazolin-3-one; and / or

[0028] The stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, silica white, talc powder, montmorillonite and starch; and / or

[0029] The synergist is selected from synergistic phosphorus and synergistic ether; and / or

[0030] The carrier is selected from one or more of ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils and vegetable oil derivatives.

[0031] By optimizing the contents of the active ingredients and adjuvants in the pesticide composition, the present invention enables a better balance between toxicity and residue, enhances the pesticidal effect, reduces the dosage of pesticides, and lowers the cost.

[0032] Furthermore, the bactericidal composition can be prepared into a pesticide-acceptable formulation dosage form, and the formulation dosage form is a seed treatment suspension, a seed treatment dry powder, a microemulsion, an emulsion in water, a suspension, a dispersible oil suspension, a soluble solution, an emulsifiable concentrate, a suspoemulsion, a microcapsule suspension, a water-dispersible granule, a wettable powder, a granule;

[0033] Furthermore, the formulation dosage form is a seed treatment suspension, a microemulsion, an emulsifiable concentrate, a suspension, an emulsion in water, a water-dispersible granule.

[0034] The present invention also discloses the application of the bactericidal composition as described above in the prevention and treatment of diseases caused by Fusarium fungi.

[0035] Furthermore, the Fusarium fungi are Fusarium oxysporum, Fusarium solani, Fusarium moniliforme, Fusarium graminearum, Fusarium pseudograminearum, Fusarium equiseti, Fusarium culmorum, Fusarium avenaceum, Fusarium proliferatum;

[0036] Even further, the Fusarium fungi are Fusarium moniliforme, Fusarium graminearum, Fusarium pseudograminearum, Fusarium equiseti, Fusarium culmorum.

[0037] The beneficial effects of the present invention are as follows:

[0038] 1) The pesticide composition of the present invention is rationally compounded with active ingredients having different action mechanisms, and has an obvious effect on delaying the development of pathogen resistance;

[0039] 2) The pesticide composition of the present invention has excellent control effects on Fusarium fungi, has a significant synergistic effect, and effectively reduces the harm of Fusarium fungi to plants. Detailed implementation manners

[0040] In order to make the purpose, advantages and technical solutions of the present invention clearer, the present invention uses the following formulation preparation examples and specific examples to explain the technical solutions of the present invention. However, the protection scope of the present invention should not be limited by the specific implementation manners described herein.

[0041] Formulation preparation examples:

[0042] Preparation Example 1: 16% Cyclobutrifluram·Tebuconazole Seed Treatment Suspension Concentrate (1:3)

[0043] Formulation composition: Cyclobutrifluram 4%, Tebuconazole 12%, Isotridecyl Polyoxyethylene Ether 1%, Styrylphenol Polyoxyethylene Ether Phosphate 2%, Sodium Lignosulfonate 2%, Polyacrylate Emulsion 1%, Xanthan Gum 0.2%, Rose Red Pigment 5%, Ethylene Glycol 5%, Magnesium Aluminum Silicate 1%, Organosilicon Defoamer 0.5%, Sodium Benzoate 1%, Deionized water to make up the balance;

[0044] Preparation method: According to the ratio, the active ingredient, auxiliary agent and water are mixed evenly by high-shear mixing and stirring, and then sanded for 2.5 h by a sand mill to make the average particle size reach 1 - 5 microns, then the seed treatment suspension concentrate can be obtained.

[0045] Preparation Example 2: 9% Cyclobutrifluram·Tebuconazole Microemulsion (1:5)

[0046] Formulation composition: Cyclobutrifluram 1.5%, Tebuconazole 7.5%, Xylene 13%, Cyclohexanone 20%, Alkylphenol Polyoxyethylene Ether 12%, EO-PO Block Copolymer 3%, Sodium Styrylphenol Polyoxyethylene Ether Sulfate 2%, Ethylene Glycol 5%, Organosilicon Defoamer 0.05%, Deionized water to make up the balance;

[0047] Preparation method: The active ingredient, solvent, emulsifier, etc. are mixed evenly to prepare the oil phase, the antifreeze and water are mixed evenly to prepare the water phase, the oil phase is added to the water phase under stirring and stirred evenly, and then sheared for 10 min, and then the organosilicon defoamer is added and stirred evenly to obtain small droplets with oil phase particles of 0.01 - 0.1 microns, that is, the microemulsion of the present invention is prepared.

[0048] Preparation Example 3: 12% Cyclobutrifluram·Tebuconazole Emulsifiable Concentrate (7:1)

[0049] Formulation composition: Cyclobutrifluram 10.5%, Tebuconazole 1.5%, Propylene Carbonate 12%, Calcium Dodecylbenzenesulfonate 4%, Guerbet Alcohol Polyoxyethylene Ether 10%, N,N-Dimethylformamide 15%, Cyclohexanone 15%, Solvent oil to make up the balance;

[0050] Preparation method: The metered active ingredient, solvent and cosolvent are added to a mixing kettle and stirred to dissolve, then the emulsifier is added, and the balance is made up with the remaining solvent, and stirred evenly in a stirring kettle, and then filtered to obtain the emulsifiable concentrate required by the present invention.

[0051] Preparation Example 4: 12% Cyclobutrifluram · Metconazole Suspension Concentrate (5:1)

[0052] Formulation composition: Cyclobutrifluram 10%, Metconazole 2%, Dioctyl Sulfosuccinate 3%, EO-PO Block Copolymer 2%, Sodium Alkylphenol Polyoxyethylene Ether Methyl Ether Condensate Sulfate 3%, Sodium Polycarboxylate 1%, Organosilicon Defoamer 0.5%, Xanthan Gum 0.2%, Magnesium Aluminum Silicate 1%, Ethylene Glycol 5%, Sodium Benzoate 0.5%, Deionized water to make up the balance;

[0053] Preparation method: According to the formulation ratio, the active ingredient, surfactant and other functional auxiliaries are placed in the reaction kettle in sequence, mixed evenly with water, and then subjected to high-speed shearing, wet grinding, and finally homogenized and filtered to obtain the suspension concentrate product.

[0054] Preparation Example 5: 21% Cyclobutrifluram · Metconazole Water Dispersible Granules (1:6)

[0055] Formulation composition: Cyclobutriflura 3%, Metconazole 18%, Sodium Lauryl Sulfate 2%, Naphthalene Sulfonate Formaldehyde Condensate 8%, Sodium Polycarboxylate 2%, White Sugar 5%, Kaolin to make up the balance;

[0056] Preparation method: According to the formulation ratio of the example, the active ingredient is added to the carrier, and surfactants and other functional auxiliaries are added thereto, mixed, and after air flow pulverization, 10-25% of water is added, and then kneaded, granulated, dried, and screened to obtain the water dispersible granule product; or the pulverized powder is sprayed with water, granulated, dried in a fluidized bed granulator, and then screened to obtain the product.

[0057] Preparation Example 6: 12% Cyclobutrifluram · Metconazole Emulsion in Water (1:1)

[0058] Formulation composition: Cyclobutrifluram 6%, Metconazole 6%, Glycerol Fatty Acid Ester Polyoxyethylene Ether 3%, Polyoxyethylene Sorbitan Monooleate 3%, Cyclohexanone 20%, Xanthan Gum 0.2%, Ethylene Glycol 5%, Urea 2%, Sodium Sorbate 0.2%, Organosilicon Defoamer 0.5%, Deionized water to make up the balance;

[0059] Preparation method: According to the formulation ratio of the example, the active ingredient is dissolved in the solvent and emulsifiers are added to make a homogeneous oil phase, and deionized water, antifreeze, etc. are mixed together to form a homogeneous water phase; under high-speed shearing, the oil phase is added to the water phase, and finally thickeners and defoamers are added to form a well-dispersed emulsion in water product.

[0060] Preparation Example 7: 18% Cyclobutrifluram·Triticonazole Seed Treatment Suspension Concentrate (1:8)

[0061] Formulation composition: Cyclobutrifluram 2%, Triticonazole 16%, glycerol fatty acid ester polyoxyethylene ether 2%, fatty alcohol ethylene oxide-propylene oxide copolymer 2%, sodium lignosulfonate 2%, magnesium aluminum silicate 1%, xanthan gum 0.3%, polyacrylic acid emulsion 1%, rose pigment 5%, propylene glycol 5%, silicone defoamer 0.5%, Kathon 0.1%, deionized water to make up the balance.

[0062] Preparation method: The same as Preparation Example 1.

[0063] Preparation Example 8: 21% Cyclobutrifluram·Triticonazole Seed Treatment Suspension Concentrate (2:1)

[0064] Formulation composition: Cyclobutrifluram 14%, Triticonazole 7%, sodium octylphenol polyoxyethylene ether sulfonate 1%, sodium polycarboxylate 1%, alkylaryl polyoxyethylene polyoxypropylene ether 2%, sodium fatty alcohol polyoxyethylene ether sulfate 3%, magnesium aluminum silicate 1%, xanthan gum 0.2%, polyacrylic acid emulsion 1%, rose pigment 6%, propylene glycol 5%, silicone defoamer 0.5%, sodium p-hydroxybenzoate 0.2%, deionized water to make up the balance.

[0065] Preparation method: The same as Preparation Example 1.

[0066] Preparation Example 9: 22% Cyclobutrifluram·Triticonazole Seed Treatment Suspension Concentrate (10:1)

[0067] Formulation composition: Cyclobutrifluram 20%, Triticonazole 2%, sodium alkylphenol polyoxyethylene ether methyl ether condensate sulfate 1%, sodium alkylnaphthalene sulfonate 1%, alkylphenol polyoxyethylene ether 3%, sodium alkyl polyoxyethylene ether sulfate 3%, magnesium aluminum silicate 1%, xanthan gum 0.2%, polyacrylic acid emulsion 1%, rose pigment 6%, glycerol 5%, silicone defoamer 0.5%, potassium benzisothiazolinone 0.1%, deionized water to make up the balance;

[0068] Preparation method: The same as Preparation Example 1.

[0069] Example 1: Indoor Activity Test of the Compound of Cyclobutrifluram with Any One of Carboxin, Metconazole or Triticonazole against Gibberella zeae

[0070] Test basis: The test refers to the agricultural industry standard of the People's Republic of China NY / T 1156.2-2006 "Pesticide Bioassay in the Laboratory - Guidelines for Fungicides - Part 2: Inhibition of Mycelial Growth of Pathogenic Fungi - Petri Dish Method".

[0071] Test target: Fusarium pseudograminearum.

[0072] Instruments and equipment: autoclave, laminar flow hood, constant temperature light incubator, electric drying oven, ten-thousandth electronic balance, pipette, alcohol lamp, small beaker, volumetric flask, Erlenmeyer flask, Petri dish (Φ9 cm), borer, inoculator (Φ0.6 cm), ruler, etc.

[0073] Test agents: 97% prothioconazole technical, 95% metconazole technical, 95% triadimefon technical, 90% Cyclobutrifluram technical. All the above agents are provided by the Group R & D Center.

[0074] Agent preparation: Dissolve the above technical agents with acetone respectively, and then dilute them with 0.1% Tween 80 aqueous solution to prepare single-agent stock solutions. Design different ratios according to the mixing purpose and agent activity, and prepare the required series of mass concentrations for each single agent and each group of mixed agents.

[0075] Test repetition: For each concentration of the test agent, 4 Petri dishes are used, 1 Petri dish for each repetition, with a total of 4 repetitions. Use 0.1% Tween 80 aqueous solution without the agent as the blank control.

[0076] Agent treatment: Under aseptic operation conditions, quantitatively add the pre-melted and sterilized PDA medium into a sterile conical flask according to the test treatment. Sequentially and quantitatively pipette 10 mL of the prepared treatment solutions of each concentration from low to high, and add them into the above conical flask respectively. Shake well, and then pour an equal amount into the Petri dish to make the corresponding concentration of drug-containing plates. Set 0.1% Tween 80 aqueous solution without adding the agent as the blank control, with 4 repetitions for each treatment.

[0077] Inoculation: Cut the mycelial cake from the edge of the colony of the pre-cultured Fusarium pseudograminearum under aseptic conditions with a sterilized borer, and inoculate the mycelial cake in the center of the drug-containing plate with an inoculator. Cover the Petri dish lid and place it in a constant temperature light incubator at 26°C for dark culture.

[0078] Data investigation: Investigate the growth of the pathogenic bacteria mycelium according to the growth of the mycelium in the blank control Petri dish. Measure the colony diameter with a ruler, with the unit of centimeter (cm). Measure the diameter of each colony once with the cross method, take the average value, and record the original data of all repetitions of each treatment.

[0079] Data statistics and analysis: According to the investigation results, calculate the mycelial growth inhibition rate of each treatment concentration against the test target bacteria, with the unit of percentage (%). Keep the calculation result to two decimal places.

[0080] D = D1 - D2

[0081] In the formula:

[0082] D —— Colony growth diameter;

[0083] D1 —— Colony diameter;

[0084] D2 —— Mycelium cake diameter.

[0085]

[0086] In the formula:

[0087] I —— Mycelium growth inhibition rate;

[0088] D0 —— Colony growth diameter of blank control;

[0089] D T —— Colony growth diameter treated with medicament.

[0090] Analyze with DPS statistical analysis system to obtain the virulence regression line and EC 50 value, and evaluate the activity of the tested medicament on the biological test materials.

[0091] Sun Yunpei method: Evaluate the synergistic effect of medicament mixture according to the co-toxicity coefficient (CTC). When the co-toxicity coefficient CTC of the mixture ≥ 120, it shows a synergistic effect; when CTC ≤ 80, it shows an antagonistic effect; when 80 < CTC < 120, it shows an additive effect.

[0092] Calculation of the co-toxicity coefficient (CTC value) of the mixture:

[0093]

[0094] In the formula:

[0095] ATI —— Measured virulence index of the mixture;

[0096] S —— EC 50 of the standard medicament, in milligrams per liter (mg / L);

[0097] M —— EC 50 of the mixture, in milligrams per liter (mg / L).

[0098] TTI = TI A ×P A +TI B ×P B

[0099] In the formula:

[0100] TTI —— Theoretical virulence index of the mixture;

[0101] TI A —— Virulence index of medicament A;

[0102] P A —— The percentage content of Agent A in the mixture, in percentage (%)

[0103] TI B —— The toxicity index of Agent B

[0104] P B —— The percentage content of Agent B in the mixture, in percentage (%)

[0105]

[0106] Where:

[0107] CTC - Co-toxicity coefficient

[0108] ATI - The measured toxicity index of the mixture

[0109] TTI - The theoretical toxicity index of the mixture

[0110] The test results are shown in the following table:

[0111] Table 1 Indoor bioactivity determination test of the combination of Cyclobutrifluram and ipconazole against Fusarium pseudograminearum

[0112]

[0113] Table 2 Indoor bioactivity determination test of the combination of Cyclobutrifluram and metconazole against Fusarium pseudograminearum

[0114]

[0115] Table 3 Indoor bioactivity determination test of the combination of Cyclobutrifluram and triadimefon against Fusarium pseudograminearum

[0116]

[0117]

[0118] As can be seen from Table 1-3 above, when Cyclobutrifluram is rationally compounded with any one of the triazole fungicides, namely, ipconazole, metconazole or tetraconazole, it has a good control effect on Fusarium pseudograminearum. When the mass ratio of Cyclobutrifluram to ipconazole is 1:50 to 48:1, it shows a synergistic effect on Fusarium pseudograminearum; when the mass ratio of Cyclobutrifluram to metconazole is 1:40 to 55:1, the combined effect shows a synergistic effect; when the mass ratio of Cyclobutrifluram to tetraconazole is 1:32 to 48:1, the combined effect on Fusarium pseudograminearum shows a synergistic effect.

[0119] Example 2: Indoor Activity Test of the Compound of Cyclobutrifluram with Any One of Ipconazole, Metconazole or Tetraconazole against Bakanae Disease of Rice

[0120] Test Basis: The test refers to the agricultural industry standard of the People's Republic of China, NY / T 1156.2-2006, "Pesticide Bioassay in the Laboratory - Guidelines for Fungicides - Part 2: Inhibiting the Mycelial Growth of Pathogenic Fungi - Petri Dish Method".

[0121] Test Strain: Fusarium moniliforme, provided by Shenyang Research Institute of Chemical Industry.

[0122] Instrument and Equipment: High-pressure steam sterilizer, laminar flow hood, incubator, electrothermal forced air drying oven, ten-thousandth electronic balance, pipettor, alcohol lamp, beaker (50 mL), volumetric flask, Erlenmeyer flask (100 mL), petri dish (Φ9 cm), punch (Φ0.6 cm), inoculator, ruler, etc.

[0123] Test Target Culture Conditions: Transfer the Fusarium moniliforme stored at 4°C in the refrigerator indoors to the potato dextrose agar medium and place it in a 25°C incubator for dark culture for 5 days to activate it for standby.

[0124] Test Agents: 97% ipconazole technical, 95% metconazole technical, 95% tetraconazole technical, 90% Cyclobutrifluram technical, and all the above agents are provided by the R & D Center of the Group.

[0125] Agent Preparation: Dissolve the above technical agents in acetone respectively to prepare high-concentration mother liquors, and then dilute them with 0.1% Tween 80 aqueous solution to prepare single-agent mother liquors. According to the compounding purpose and agent activity, design different ratios, and prepare the required series of mass concentrations for each single agent and each group of mixed agents.

[0126] Test Repetition: For each concentration of the test agent, use 4 petri dishes, 1 petri dish for each repetition, with a total of 4 repetitions. Use 0.1% Tween 80 aqueous solution without the agent as the blank control.

[0127] Drug treatment: Under aseptic operation conditions, use a pipette to add 5 mL of liquid medicine with different concentrations into pre-calibrated sterile Erlenmeyer flasks respectively. Then add the medium melted and cooled to an appropriate temperature into the Erlenmeyer flasks. After shaking well, pour an equal amount into 4 Petri dishes to make PDA plates containing drugs with corresponding concentrations.

[0128] Inoculation: Cut out fungal discs from the edge of the colony of pre-cultured Fusarium moniliforme under aseptic conditions using a sterilized borer. Use an inoculator to inoculate the fungal discs in the center of the drug-containing plates, cover the lids, and place them in a constant temperature incubator at 25 °C for dark culture.

[0129] Data investigation: Conduct experimental investigation when the colony in the control treatment grows to 2 / 3 - 4 / 5 of the diameter of the Petri dish. Measure the colony diameter (cm) with a ruler. Measure the diameter of each colony once using the cross method and take the average value.

[0130] Data statistics and analysis: According to the investigation results, calculate the mycelial growth inhibition rate of each treatment concentration on the test target fungus, and calculate the result to two decimal places in percentage (%).

[0131] D = D1 - D2

[0132] In the formula:

[0133] D - - Colony growth diameter;

[0134] D1 - - Colony diameter;

[0135] D2 - - Fungal disc diameter.

[0136]

[0137] In the formula:

[0138] I - - Mycelial growth inhibition rate;

[0139] D0 - - Colony growth diameter of the blank control;

[0140] D T - - Colony growth diameter of the drug treatment.

[0141] Analyze with the DPS statistical analysis system to obtain the virulence regression line and EC 50 value, and evaluate the activity of the test drugs on the biological test materials.

[0142] Sun Yunpei method: Evaluate the synergistic effect of drug mixtures according to the co-toxicity coefficient (CTC). When the co-toxicity coefficient CTC of the compound ≥ 120, it shows a synergistic effect; when CTC ≤ 80, it shows an antagonistic effect; when 80 < CTC < 120, it shows an additive effect.

[0143] Calculation of the co-toxicity coefficient (CTC value) of the mixture:

[0144]

[0145] Where:

[0146] ATI——Actual toxicity index of the mixture;

[0147] S——EC of the standard agent 50 , in milligrams per liter (mg / L);

[0148] M——EC of the mixture 50 , in milligrams per liter (mg / L).

[0149] TTI = TI A ×P A +TI B ×P B Where:

[0150] TTI——Theoretical toxicity index of the mixture;

[0151] TI A ——Toxicity index of agent A;

[0152] P A ——Percentage content of agent A in the mixture, in percentage (%);

[0153] TI B ——Toxicity index of agent B;

[0154] P B ——Percentage content of agent B in the mixture, in percentage (%).

[0155]

[0156] Where:

[0157] CTC——Co-toxicity coefficient;

[0158] ATI——Actual toxicity index of the mixture;

[0159] TTI——Theoretical toxicity index of the mixture.

[0160] The test results are shown in the following table:

[0161] Table 4 Indoor bioactivity determination test of cyclobutrifluram and ipconazole against Fusarium moniliforme

[0162]

[0163] Table 5 Indoor bioactivity test of cyclobutrifluram and metconazole against Fusarium moniliforme

[0164]

[0165]

[0166] Table 6 Cyclobutrifluram and trichlorfonazole combined with indoor biological activity test on Fusarium moniliforme

[0167]

[0168] As can be seen from Tables 4-6 above, a reasonable combination of Cyclobutrifluram and any of the triazole fungicides cyproconazole, metconazole or trichlorfon has a good control effect on Fusarium moniliforme. When the mass ratio of Cyclobutrifluram to cyproconazole is 1:30-48:1, it has a synergistic effect on Fusarium moniliforme; when the mass ratio of Cyclobutrifluram to metconazole is 1:45-40:1, the combined effect is a synergistic effect; when the mass ratio of Cyclobutrifluram to trichlorfonazole is 1:25-30:1, the combined effect on Fusarium moniliforme is a synergistic effect.

[0169] Example 3: Field test on controlling rice bakanae disease

[0170] Test basis: The test refers to GB / T 17980.104-2004 "Guidelines for Field Efficacy Tests of Pesticides (II) Part 104: Bactericidal and Control of Rice Seedling Disease".

[0171] Test target: Rice seedling pathogen.

[0172] Experimental crops: rice.

[0173] Experimental design: The plots of experimental agents, control agents and blank controls were arranged in random blocks. The experiment set up 7 agent soaking treatments, and clear water was used as a blank control. Each treatment area of the seedbed was 10m 2 , the field planting area is 30m 2 , each treatment was repeated 4 times.

[0174] The experiment was conducted on May 31, 2022, with seeds soaked, germinated on June 2, and sown on June 5. Prepare the solution according to the experimental design concentration for each treatment and stir it thoroughly, pour in dry seeds, soak the seeds for 48 hours for germination, and keep the seeds out of the water during the soaking period. Sow in seedling trays 3 days later, and strictly control the temperature and humidity of the seedbed according to the rice technical regulations. Move the seedling tray to the seedling field after germination, and move it to the field when the seedlings are 30 days old.

[0175] Survey items: When all rice seedlings on the seedbed have emerged, investigate the emergence rate of each treatment area. The disease index survey is conducted once before transplanting and once during the heading stage of field rice.

[0176] Before transplanting the seedlings, take samples at 5 points in each plot in the seedling field, investigate 200 seedlings at each point, investigate the disease rate, and calculate the control effect;

[0177] In the field investigation at the booting stage, 5 random sampling points were taken in each plot, 20 clusters were investigated at each point, the number of diseased plants was recorded, and the diseased plant rate and control effect of each treatment were calculated.

[0178] Calculation method of drug efficacy:

[0179]

[0180] The test results are as follows:

[0181] Table 7 Field control of rice seedling disease test results

[0182]

[0183]

[0184] As can be seen from Table 7, the pesticide composition of the present invention exhibits significant control effects on rice seedling blanching disease. Compared with the blank control agent treatment, the emergence rate is basically the same as the blank control, indicating that the rice seeds will not be damaged by the preparation examples after soaking the rice seeds. Compared with the single-agent fungicide, the diseased plant rate of each compound preparation is reduced, and it has a good control effect.

[0185] Example 4: Field test on prevention and control of wheat stem rot

[0186] Test target: wheat stem rot pathogen.

[0187] Experimental crop: wheat.

[0188] Application method: The test application is divided into two methods: root spraying and seed mixing with pesticides.

[0189] Experimental design: The corresponding treatments were set according to the experimental method; 5 treatments were set for the root spray test and 8 treatments were set for the seed dressing test. The above experiments were repeated 4 times. Each experimental plot was arranged in a random block group with an area of 20m 2 .

[0190] Test method: The root spray test was carried out 3 and a half months after wheat planting. Root spray was carried out at the early stage of wheat stem base rot, and then sprayed again after an interval of 7 days, for a total of 2 applications.

[0191] Before wheat planting, the wheat seeds were treated with medicaments according to the experimental design.

[0192] Investigation method and time:

[0193] The experiment was investigated at the milk ripening stage of wheat. Five random sampling points were taken in each plot, and 100 plants were selected at each point to investigate the number of diseased plants at each level. The grading criteria are as follows:

[0194] The disease severity was graded according to the following criteria:

[0195] Grade 0: The whole plant has no browning symptom;

[0196] Grade 1: Browning appears at the root;

[0197] Grade 3: Browning and rot appear at the first stem node above the ground;

[0198] Grade 5: Browning and rot appear at the second stem node above the ground;

[0199] Grade 7: The lesion exceeds the second stem node, but there is no white ear;

[0200] Grade 9: The lesion exceeds the second stem node, and there is a white ear;

[0201] Calculation formulas for disease index and control effect:

[0202]

[0203] The test results are as follows:

[0204] Table 8 Test results of medicament seed dressing application for wheat basal stalk rot

[0205]

[0206] Table 9 Test results of root spraying application for wheat basal stalk rot

[0207]

[0208] The field efficacy showed that the mixture of cyclobutrifluram with ipconazole, metconazole and triadimefon showed significantly better antibacterial activity against wheat basal stalk rot than single agents.

[0209] In summary, through indoor toxicity determination and field efficacy tests, it can be seen that the pesticide composition of the present invention has good control effect on Fusarium, is safe for target crops, has significant control effect, is superior to single agents in delaying the generation of drug resistance and prolonging the long-lasting effect, can effectively reduce costs and reduce pesticide residues.

[0210] Although the present application describes specific embodiments in detail by way of examples, the disclosure of the present application may adopt various modifications and alternative forms. However, it should be understood that the disclosure of the present application is not limited to the specific forms disclosed. On the contrary, the disclosure of the present application covers all modifications, equivalents and alternative forms within the scope of the disclosure of the present application, and the scope of the present application is defined by the appended claims and their legal equivalents.

Claims

1. A bactericidal composition, characterized in that, The described bactericidal composition contains active ingredient A and active ingredient B. Active ingredient A is Cyclobutrifluram, and active ingredient B is metconazole. The mass ratio of active ingredient A to active ingredient B is 1:50 to 55:

1.

2. The bactericidal composition according to claim 1, wherein The mass ratio of active ingredient A to active ingredient B is 1:40 to 55:

1.

3. The bactericidal composition according to claim 2, wherein The mass ratio of active ingredient A to active ingredient B is 1:30 to 48:

1.

4. The bactericidal composition according to claim 1, characterized in that, Calculated based on 100 wt%, the total weight of the bactericidal combination, and the total weight of active ingredient A and active ingredient B accounts for 0.1% to 80% of the total weight of the bactericidal composition.

5. The bactericidal composition according to claim 1, characterized in that, In addition to the active ingredients, the described bactericidal composition also contains agriculturally acceptable auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreezing agents, defoaming agents, solvents, preservatives, stabilizers, synergists, binders, fillers, or carriers.

6. The bactericidal composition according to claim 1, wherein The described bactericidal composition can be prepared into pesticide-acceptable formulation dosage forms, which are seed treatment suspension concentrates, seed treatment dry powders, microemulsions, emulsifiable concentrates, suspension concentrates, dispersible oil suspension concentrates, soluble solutions, emulsifiable concentrates, suspension emulsions, microcapsule suspension concentrates, water-dispersible granules, wettable powders, granules.

7. The bactericidal composition according to claim 6, wherein The formulation dosage forms are seed treatment suspension concentrates, microemulsions, emulsifiable concentrates, suspension concentrates, emulsifiable concentrates, water-dispersible granules.

8. Use of the bactericidal composition according to any one of claims 1-7 for controlling diseases caused by Fusarium fungi.

9. The application according to claim 8, characterized in that, The Fusarium fungi are Fusarium oxysporum, Fusarium solani, Fusarium moniliforme, Fusarium graminearum, Fusarium pseudograminearum, Fusarium equiseti, Fusarium culmorum, Fusarium avenaceum, Fusarium proliferatum.

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