Compound feed additive for improving pet bones and preparation method thereof

Abstract

The invention relates to the technical field of pet feed additives, in particular to a compound feed additive for improving pet bones and a preparation method of the compound feed additive. Comprising the following components: 8% of chondroitin sulfate, 10% of glucosamine hydrochloride, 0.2% of dimethyl sulfone, 0.2% of collagen, 0.4% of vitamin D3, 0.8% of vitamin E, 0.4% of zinc gluconate, 0.4% of manganese gluconate, 0.4% of calcium gluconate, a taste adjusting component and other components. According to the pet feed additive, the chondroitin sulfate, the glucosamine hydrochloride, the dimethyl sulfone and the collagen are used as main components, and the vitamin D3, the vitamin E, the zinc gluconate, the manganese gluconate and the calcium gluconate are used as secondary components, so that the bone health problem in the growth process of pets can be effectively improved; therefore, the condition of dysplasia of the pets in a young state is avoided, and meanwhile, the conditions of osteoporosis and joint degeneration of the adult pets can be avoided.

Description

Technical Field

[0001] The present invention relates to the technical field of pet feed additives, and specifically relates to a compound feed additive for improving pet bones and a preparation method thereof. Background Art

[0002] With the continuous improvement of people's attention to the health and nutritional needs of pets, the pet compound feed additive industry is developing towards a more natural, healthy, personalized and intelligent direction. Adding additives to pet feed is a small amount or trace amount of substances added to pet feed to meet the special nutritional and health needs of pets, and these additives play an important role in the modern pet feed industry.

[0003] Pet bone health problems have attracted increasing attention. Problems such as poor pet bone development and osteoporosis not only affect the pet's motor ability but may also lead to serious health problems. However, during the use of existing pet compound feed additives, they only meet the pet's daily nutritional needs, and there is a lack of additives specifically for improving pet bone health needs, which is likely to cause poor pet development, osteoporosis and joint degeneration. Summary of the Invention

[0004] The purpose of the present invention is to provide a compound feed additive for improving pet bones and a preparation method thereof, so as to solve the problem that during the use of pet compound feed additives as mentioned in the above background art, they only meet the pet's daily nutritional needs, and there is a lack of additives specifically for improving pet bone health needs, which is likely to cause poor pet development, osteoporosis and joint degeneration.

[0005] To achieve the above purpose, the present invention provides the following technical solution: a compound feed additive for improving pet bones and a preparation method thereof, including: the components of the compound feed additive include chondroitin sulfate 8%, glucosamine hydrochloride 10%, dimethyl sulfone 0.2%, collagen 0.2%, vitamin D3 0.4%, vitamin E 0.8%, zinc gluconate 0.4%, manganese gluconate 0.4%, calcium gluconate 0.4%, flavor regulating components and other components.

[0006] Preferably, the process for preparing chondroitin sulfate is as follows: Select cartilage raw materials, clean them, remove the attached muscle and fat tissues and cut them into small pieces. Then, immerse them in water and heat up to 80-100 °C, and boil for 12-24 hours. After that, put them into a steaming kettle and steam at 100 °C for more than 4 hours. Immerse them in water again, add sodium chloride at 3% of the water volume, adjust the pH to 8-9 with an alkali, then heat up to 55-60 °C, add an appropriate amount of alkaline protease, keep the pH at 8-9 and stir for 4-6 hours while maintaining the temperature. Finally, heat up to boiling for 12-18 minutes, filter, adsorb the enzyme hydrolysate with resin, then elute, collect the eluate, adjust the pH of the eluate to 6-7, add alcohol to make the alcohol mass concentration of the solution reach 60%, precipitate for more than 16 hours, dissolve the precipitate in water, heat up to 40-60 °C, adjust the pH to 8-9, add an appropriate amount of hydrogen peroxide for decolorization, cool the decolorized solution to 0-30 °C, adjust the pH to 1-4 with hydrochloric acid, add an appropriate amount of sodium nitrite, stir for 1-3 hours, adjust the pH of the solution after impurity removal to 6-7, add sodium chloride at 5% of its volume, filter and then add alcohol to make the alcohol mass concentration of the solution reach 60%, precipitate for more than 16 hours, discard the upper layer of alcohol, collect the precipitate, and perform dehydration and drying treatment to finally obtain the chondroitin sulfate finished product.

[0007] Preferably, the process for preparing glucosamine hydrochloride is as follows: Clean the shrimp and crab shells, soak them in 1.5N hydrochloric acid for decalcification to remove the inorganic salts in the shells, rinse to neutrality and dry. Then, place the outer shells in a 2.5N NaOH solution and remove the protein at 60 °C, rinse to neutrality, and dry to obtain chitin. Put the chitin into a three-necked flask, add concentrated hydrochloric acid, and carry out a hydrolysis reaction by heating in a water bath. After completion, filter to obtain the filtrate and cool it for crystallization to obtain the crude product. Then, decolorize it with activated carbon and recrystallize it in an acetone system, and vacuum dry to obtain white powdery glucosamine hydrochloride.

[0008] Preferably, the process for preparing dimethyl sulfone is as follows: First, prepare dimethyl sulfoxide and hydrogen peroxide, and add an appropriate amount of 2,6-naphthalenedicarboxylic acid catalyst. Pump the mixed solution into a fixed-bed reactor filled with the catalyst and react at 20-120 °C. The mixed solution flows along the catalyst bed layer in the fixed bed. After the reaction is completed, collect the reaction solution at the outlet of the fixed bed, cool and crystallize the reaction solution, and then obtain dimethyl sulfone crystals by centrifugal separation or filtration. Dry it in a vacuum dryer to obtain a high-purity dimethyl sulfone product.

[0009] Preferably, the process for preparing collagen is as follows: Select fresh animal tissues, remove fat and fascia, soak them in a dilute alkali solution, and then rinse them with distilled water until the alkali solution is completely removed and they are dried. After that, chop them up and add proteolytic enzymes, slowly stir under low-temperature conditions, then centrifuge with a high-speed refrigerated centrifuge, take the supernatant to obtain a crude collagen solution, add hydrogen peroxide solution, mix evenly and let it stand, adjust the pH value of the solution to about 5 with trisodium citrate solution, centrifuge again to remove the precipitate, add sodium chloride to the remaining solution for salting out, slowly stir and then let it stand overnight under low-temperature conditions to precipitate collagen. Put the precipitate obtained by salting out into a dialysis bag, dialyze with acetic acid solution for one day first, and then dialyze with distilled water for several days, changing the dialysis solution 2 - 3 times a day to remove salts and other small molecule impurities, and finally obtain a high-purity collagen solution. Concentrate the purified collagen solution to increase its concentration, and then convert it into powder form through spray drying technology for easy storage and use.

[0010] Preferably, the flavor-adjusting components include adding 2% chicken liver powder and 1% beef powder.

[0011] Preferably, the other components include 1.5% yucca extract, 59.7% starch, and 15% lactose.

[0012] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0013] 1. The feed additive with chondroitin sulfate, glucosamine hydrochloride, dimethyl sulfone, and collagen as the main components and vitamin D3, vitamin E, zinc gluconate, manganese gluconate, and calcium gluconate as the secondary components can effectively improve the bone health problems during the growth of pets, avoid the underdevelopment of pets in the juvenile state, and at the same time avoid the formation of osteoporosis and joint degeneration in adult pets, thereby enhancing the bone strength during the growth of pets, enabling the normal activities of pets, reducing the injuries of pets, and prolonging the lifespan of pets. Detailed embodiments

[0014] The described embodiments are only a part of the embodiments of the present invention, rather than all embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts fall within the scope of protection of the present invention.

[0015] A compound feed additive for improving pet bones and its preparation method, including: The components of the compound feed additive include 8% chondroitin sulfate, 10% glucosamine hydrochloride, 0.2% dimethyl sulfone, 0.2% collagen, 0.4% vitamin D3, 0.8% vitamin E, 0.4% zinc gluconate, 0.4% manganese gluconate, 0.4% calcium gluconate, flavor regulating components and other components. The combination of chondroitin sulfate and glucosamine can protect the joint cartilage of pets and promote cartilage repair, and the best effect is achieved when the ratio is 5:4. The combination of collagen and dimethyl sulfone can promote the recovery of damaged bone joints, provide organic sulfur, and accelerate tissue repair. The addition of various vitamins and mineral elements can synergistically promote bone health and improve the immunity of pets.

[0016] Further, the process for preparing chondroitin sulfate is as follows: Select cartilage raw materials, wash them, remove the attached muscle and fat tissues and cut them into small pieces. Then add water to submerge and heat up to 80 - 100 °C, cook for 12 - 24 hours. After that, put them into a steaming kettle and steam at 100 °C for more than 4 hours. Add water to submerge again, add 3% sodium chloride based on the volume of water, adjust the pH to 8 - 9 with alkali, then heat up to 55 - 60 °C, add an appropriate amount of alkaline protease, keep the pH at 8 - 9 and stir while keeping warm for 4 - 6 hours. Finally, heat up to boiling for 12 - 18 minutes, filter, adsorb the enzymatic hydrolysate with resin, then elute, collect the eluate, adjust the pH of the eluate to 6 - 7, add alcohol to make the alcohol mass concentration of the solution reach 60%, precipitate for more than 16 hours, dissolve the precipitate in water, heat up to 40 - 60 °C, adjust the pH to 8 - 9, add an appropriate amount of hydrogen peroxide for decolorization, cool the decolorized solution to 0 - 30 °C, adjust the pH to 1 - 4 with hydrochloric acid, add an appropriate amount of sodium nitrite, stir for 1 - 3 hours, adjust the pH of the impurity-removed solution to 6 - 7, add 5% sodium chloride based on its volume, filter, then add alcohol to make the alcohol mass concentration of the solution reach 60%, precipitate for more than 16 hours, discard the upper-layer alcohol, collect the precipitate, and perform dehydration and drying treatment to finally obtain the chondroitin sulfate finished product.

[0017] Further, the process for preparing glucosamine hydrochloride is as follows: Wash the shrimp and crab shells, soak them in 1.5N hydrochloric acid for decalcification to remove the inorganic salts in the shells, rinse to neutral and dry. Then place the outer shells in 2.5N NaOH solution and remove the proteins at 60 °C, rinse to neutral, and dry to obtain chitin. Put the chitin into a three-neck flask, add concentrated hydrochloric acid, and carry out a hydrolysis reaction by heating in a water bath. After completion, filter to obtain the filtrate and cool it for crystallization to obtain the crude product. Then decolorize it with activated carbon, recrystallize it in an acetone system, and vacuum dry it to obtain white powdery glucosamine hydrochloride.

[0018] Further, the process for preparing dimethyl sulfone is as follows: First, prepare dimethyl sulfoxide and hydrogen peroxide, and add an appropriate amount of 2,6-naphthalenedicarboxylic acid catalyst. Then, pump the mixed solution into a fixed-bed reactor filled with the catalyst and react at 20 - 120°C. The mixed solution flows along the catalyst bed in the fixed bed. After the reaction, collect the reaction solution at the outlet of the fixed bed, cool and crystallize the reaction solution, and then perform centrifugal separation or filtration to obtain dimethyl sulfone crystals. Dry the crystals in a vacuum dryer to obtain a high-purity dimethyl sulfone product.

[0019] Further, the process for preparing collagen is as follows: Select fresh animal tissues, remove fat and fascia, soak them in a dilute alkali solution, and then rinse with distilled water until the alkali solution is washed away and air-dried. Then, chop them and add proteolytic enzymes, slowly stir under low-temperature conditions, and then centrifuge with a high-speed refrigerated centrifuge. Take the supernatant to obtain a crude collagen solution. Then, add a hydrogen peroxide solution, mix evenly, let it stand, and adjust the pH value of the solution to about 5 with a trisodium citrate solution. Centrifuge again to remove the precipitate. Add sodium chloride to the remaining solution for salting out. After slowly stirring, let it stand overnight under low-temperature conditions to precipitate collagen. Pack the precipitate obtained by salting out into a dialysis bag, dialyze with acetic acid solution for one day first, and then dialyze with distilled water for several days, changing the dialysis solution 2 - 3 times a day to remove salts and other small-molecule impurities. Finally, obtain a high-purity collagen solution. Concentrate the purified collagen solution to increase its concentration, and then convert it into powder form through spray drying technology for easy storage and use.

[0020] Further, the flavor-adjusting components include adding 2% chicken liver powder and 1% beef powder. By adjusting the flavor, the palatability of the product can be improved, making pets more willing to eat.

[0021] Further, the other components include 1.5% yucca extract, 59.7% starch, and 15% lactose. Yucca extract has the functions of analgesia, anti-inflammation, and regulating the health of the intestinal flora, and an appropriate amount of binder is added during production.

[0022] Working principle: First, prepare the required raw materials and perform pretreatment, and pass various components through an 80-mesh sieve respectively to ensure that the particles of the components are fine and uniform. Then, weigh chondroitin sulfate, glucosamine hydrochloride, and part of the starch according to the weight ratio, add water to make a starch paste, and mix the remaining materials evenly. Then, sieve it three times again, pour the starch paste into the mixture and stir evenly. Finally, granulate with a 16-mesh sieve, dry the obtained granules at 50 - 60°C, and screen them with a 14-mesh sieve to obtain the finished product.

[0023] It is obvious to those skilled in the art that the present invention is not limited to the details of the above-described exemplary embodiments, and that the present invention can be implemented in other specific forms without departing from the spirit or essential characteristics of the present invention. Therefore, in all respects, the embodiments should be regarded as exemplary and non-restrictive. The scope of the present invention is defined by the appended claims rather than the above description. Therefore, all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be embraced within the present invention.

Claims

1. A compound feed additive for improving pet bones and its preparation method, characterized in that, Comprising: The components of the compound feed additive include 8% chondroitin sulfate, 10% glucosamine hydrochloride, 0.2% dimethyl sulfone, 0.2% collagen, 0.4% vitamin D3, 0.8% vitamin E, 0.4% zinc gluconate, 0.4% manganese gluconate, 0.4% calcium gluconate, flavor regulating components and other components.

2. The compound feed additive for improving pet bones and its preparation method according to claim 1, characterized in that, The process for preparing chondroitin sulfate is as follows: Select cartilage raw materials, wash them, remove the attached muscle and fat tissues and cut them into small pieces, then add water to submerge and heat up to 80 - 100 °C, boil for 12 - 24 hours, then put them into a steaming kettle and steam at 100 °C for more than 4 hours, add water to submerge again, add sodium chloride at 3% of the water volume, adjust the pH to 8 - 9 with an alkali, then heat up to 55 - 60 °C, add an appropriate amount of alkaline protease, keep the pH at 8 - 9 and stir while maintaining the temperature for 4 - 6 hours, finally heat up to boiling for 12 - 18 minutes, filter, adsorb the enzymatic hydrolysate with resin, then elute, collect the eluate, adjust the pH of the eluate to 6 - 7, then add alcohol to make the alcohol mass concentration of the solution reach 60%, precipitate for more than 16 hours, dissolve the precipitate in water, heat up to 40 - 60 °C, adjust the pH to 8 - 9, add an appropriate amount of hydrogen peroxide for decolorization, cool the decolorized solution to 0 - 30 °C, adjust the pH to 1 - 4 with hydrochloric acid, add an appropriate amount of sodium nitrite, stir for 1 - 3 hours, adjust the pH of the solution after impurity removal to 6 - 7, add sodium chloride at 5% of its volume, filter and then add alcohol to make the alcohol mass concentration of the solution reach 60%, precipitate for more than 16 hours, discard the upper layer of alcohol, collect the precipitate, and perform dehydration and drying treatment to finally obtain the finished product of chondroitin sulfate.

3. A compound feed additive for improving pet bones and its preparation method according to claim 1, characterized in that, The process for preparing glucosamine hydrochloride is as follows: Wash shrimp and crab shells, soak them in 1.5N hydrochloric acid for decalcification to remove the inorganic salts in the shells, rinse to neutral and dry, then place the outer shells in 2.5N NaOH solution and remove proteins at 60 °C, rinse to neutral, dry to obtain chitin, put the chitin into a three-necked flask, add concentrated hydrochloric acid, heat in a water bath for hydrolysis reaction, filter the filtrate after completion and cool it for crystallization to obtain a crude product, then decolorize with activated carbon and recrystallize in an acetone system, and vacuum dry to obtain white powdery glucosamine hydrochloride.

4. A compound feed additive for improving pet bones and its preparation method according to claim 1, characterized in that, The process for preparing dimethyl sulfone is as follows: First prepare dimethyl sulfoxide and hydrogen peroxide, and add an appropriate amount of 2,6-naphthalenedicarboxylic acid catalyst, pump the mixed solution into a fixed-bed reactor filled with the catalyst and react at 20 - 120 °C, the mixed solution flows along the catalyst bed layer in the fixed bed, collect the reaction solution at the outlet of the fixed bed after the reaction, cool and crystallize the reaction solution, then separate by centrifugation or filtration to obtain dimethyl sulfone crystals, and dry them in a vacuum dryer to obtain high-purity dimethyl sulfone products.

5. A compound feed additive for improving pet bones and its preparation method according to claim 1, characterized in that, The process for preparing collagen is as follows: Select fresh animal tissues, remove fat and fascia, soak them in a dilute alkali solution, then rinse with distilled water until the alkali solution is completely washed away and air-dry. After that, chop them up and add proteolytic enzymes, slowly stir under low-temperature conditions, then centrifuge with a high-speed refrigerated centrifuge, take the supernatant to obtain a crude collagen solution, add hydrogen peroxide solution, mix evenly and let it stand, adjust the pH value of the solution to about 5 with trisodium citrate solution, centrifuge again to remove the precipitate, add sodium chloride to the remaining solution for salting out, slowly stir and then let it stand overnight under low-temperature conditions to precipitate collagen. Put the precipitate obtained by salting out into a dialysis bag, dialyze with acetic acid solution for one day first, then dialyze with distilled water for several days, changing the dialysis solution 2 - 3 times a day to remove salts and other small-molecule impurities, and finally obtain a high-purity collagen solution. Concentrate the purified collagen solution to increase its concentration, and then convert it into powder form through spray drying technology for easy storage and use.

6. The compound feed additive for improving pet bones and its preparation method according to claim 1, characterized in that: The flavor adjustment components include adding 2% chicken liver powder and 1% beef powder.

7. A compound feed additive for improving pet bones and its preparation method according to claim 1, characterized in that: The other components include 1.5% yucca extract, 59.7% starch, and 15% lactose.