Feed additive and feed for improving quality of poultry eggs and application of feed additive and feed

Abstract

The invention provides a feed additive and feed for improving the quality of poultry eggs and application of the feed additive and the feed, and belongs to the technical field of feed. The invention provides a feed additive, which is prepared from the following components in parts by mass: 20 to 30 parts of lycopene, 10 to 20 parts of capsorubin, 20 to 30 parts of pumpkin powder and 20 to 30 parts of zeaxanthine. Lycopene and zeaxanthine have the effects of resisting oxidation, improving immunity and the like; capsorubin has the effects of resisting oxidation, resisting inflammation, enhancing immunity, resisting cancer and the like; the pumpkin powder has the effects of regulating blood sugar, promoting digestion, delaying senescence, enhancing immunity and the like. According to the feed additive provided by the invention, the lycopene, the capsorubin, the pumpkin powder and the zeaxanthine are combined for use, so that the effects of improving the egg yield of egg laying poultry, improving the egg quality and improving the nutritional value of eggs can be synergistically exerted.

Description

Technical Field

[0001] The present invention belongs to the technical field of feeds, and particularly relates to a feed additive, a feed for improving the egg quality of poultry, and their applications. Background Art

[0002] With the continuous development of the national economy, the living standards of residents have been continuously improved; with the improvement of residents' health awareness, consumption upgrading, and increased attention to food safety, the demand and consumption of high-quality livestock and poultry products by residents show an increasing trend. Eggs are one of the foods with low prices and rich nutrition in people's daily lives. With the development of society, it is necessary to not only meet people's demand for the quantity of eggs but also meet the quality requirements. Green, healthy, safe, and functional eggs have become a major trend in people's consumption.

[0003] In large-scale laying hen farming, laying hens are easily affected by various internal and external factors, resulting in stress, seriously affecting their production performance and egg quality, and even leading to their death, causing huge economic losses. For example, during the peak laying period, laying hens are easily affected by the outside world and prone to yolk peritonitis, and during the peak laying period, due to excessive egg production by laying hens, diseases such as prolapse of the oviduct and difficult egg laying may occur, thereby affecting the egg production and / or quality, etc.

[0004] In summary, the demand for high-quality livestock and poultry products by the public has increased, but laying hens are prone to economic losses due to being affected by internal and external factors during the large-scale production process. Therefore, it is crucial to produce high-quality eggs to meet the national demand and reduce economic losses in the breeding industry. At the same time, the problem of antibiotic residues in poultry bodies is also being increasingly recognized worldwide. In view of the problem of drug residues in current egg products, how to provide an effective feed additive that can replace antibiotics has become an important issue for industry researchers. Summary of the Invention

[0005] Aiming at the defects in the prior art, the purpose of the present invention is to provide a feed additive without antibiotics, which can simultaneously improve the egg production and / or quality.

[0006] The purpose of the present invention is achieved through the following technical solutions: The present invention provides a feed additive, which, by mass, comprises: 20 - 30 parts of lycopene, 10 - 20 parts of capsanthin, 20 - 30 parts of pumpkin powder, and 20 - 30 parts of zeaxanthin.

[0007] The present invention provides a preparation method of the feed additive as described in the above technical solution, comprising: Mixing lycopene, capsanthin, pumpkin powder, and zeaxanthin to obtain the feed additive.

[0008] The present invention provides a feed, which comprises the feed additive described in the above technical solution or the feed additive prepared by the preparation method described in the above technical solution.

[0009] Preferably, the feed further comprises a basal diet.

[0010] Preferably, the addition amount of the feed additive in the basal diet is 0.1 wt.% - 0.5 wt.%.

[0011] The present invention provides an application of a feed additive or a feed in the breeding of laying poultry. The feed additive is the feed additive described in the above technical solution or the feed additive prepared by the preparation method described in the above technical solution; the feed is the feed described in the above technical solution.

[0012] Preferably, the application includes any one or more of the following (1) - (4): (1) Increasing the average daily feed intake of poultry; (2) Increasing the egg production of poultry; (3) Improving the quality of eggs; (4) Increasing the nutritional value of eggs.

[0013] Preferably, the poultry includes chickens.

[0014] The present invention provides a method for increasing the egg production rate of poultry and / or improving the quality of eggs, which includes: Feeding poultry with a feed containing the feed additive described in the above technical solution or the feed additive prepared by the preparation method described in the above technical solution.

[0015] Advantages of the present invention: The present invention provides a feed additive, which, by mass fraction, comprises: 20-30 parts of lycopene, 10-20 parts of capsanthin, 20-30 parts of pumpkin powder, and 20-30 parts of zeaxanthin. In the present invention, lycopene and zeaxanthin have functions such as antioxidation and immunity improvement; capsanthin has functions such as antioxidation, anti-inflammation, immunity enhancement, and anti-cancer; pumpkin powder has functions such as blood sugar regulation, digestion promotion, aging delay, and immunity enhancement. The combined use of lycopene, capsanthin, pumpkin powder, and zeaxanthin in the feed additive provided by the present invention can synergistically exert the effects of increasing the egg production of laying poultry, improving the egg quality, and enhancing the egg nutritional value. Moreover, the raw materials in the feed additive are all plant extracts, which have the advantages of being safe, non-toxic, and having no side effects, and low production cost, and are feed additives without antibiotics. The results of the examples of the present invention show that: the feed additive can significantly increase the average daily feed intake of laying hens and increase the weekly egg production of laying hens; the feed additive can significantly improve the yolk color score, albumen height, and Haugh unit of eggs, and thus can improve the egg quality; the feed additive can significantly increase the contents of vitamin A and polyunsaturated fatty acids in eggs, and significantly increase the content of antioxidant substances in eggs, and thus is beneficial to improving the nutritional value of eggs. In summary, the feed additive provided by the present invention is beneficial to increasing the yield and / or quality of eggs. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for use in the embodiments. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0017] Figure 1 It is the yolk color map of eggs in each experimental group in the 1st week of the experiment; Figure 2 It is the yolk color map of eggs in each experimental group in the 2nd week of the experiment; Figure 3 It is the yolk color map of eggs in each experimental group in the 3rd week of the experiment; Figure 4 It is the yolk color map of eggs in each experimental group in the 4th week of the experiment; Figure 5 It is the yolk color map of eggs in each experimental group in the 5th week of the experiment; Figure 6 It is the yolk color map of eggs in each experimental group in the 6th week of the experiment; Figure 7 It is the yolk color map of eggs in each experimental group in the 7th week of the experiment; Figure 8 It is the yolk color score map of eggs in each experimental group in the 7th week of the experiment; Figure 9 It is a comparison chart of the egg yolk colors in each experimental group of eggs in Weeks 1 - 7 of the experiment. Specific implementation manners

[0018] The present invention provides a feed additive. By mass, the feed additive includes: 20 - 30 parts of lycopene, 10 - 20 parts of capsanthin, 20 - 30 parts of pumpkin powder, and 20 - 30 parts of zeaxanthin.

[0019] In the following technical solutions, the present invention does not have special limitations on the sources of each raw material, and conventional commercially available products in the art can be used.

[0020] As an optional implementation manner of the present invention, by mass, the mass of lycopene in the feed additive can be 20 - 30 parts, or can be 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or 30 parts.

[0021] As an optional implementation manner of the present invention, based on the mass of lycopene, the mass of capsanthin in the feed additive can be 10 - 20 parts, and can be 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 parts.

[0022] As an optional implementation manner of the present invention, based on the mass of lycopene, the mass of pumpkin powder in the feed additive can be 20 - 30 parts, or can be 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or 30 parts.

[0023] As an optional implementation manner of the present invention, based on the mass of lycopene, the mass of zeaxanthin in the feed additive can be 20 - 30 parts, or can be 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or 30 parts.

[0024] In the feed additive provided by the present invention, lycopene and zeaxanthin have functions such as antioxidant and immune - enhancing; capsanthin has functions such as antioxidant, anti - inflammatory, immune - enhancing, and anti - cancer; pumpkin powder has functions such as blood - sugar regulation, digestion promotion, aging delay, and immune - enhancing. Combining lycopene, capsanthin, pumpkin powder, and zeaxanthin in the feed additive provided by the present invention can synergistically play the effects of increasing the egg production of laying poultry, improving egg quality, and increasing egg nutritional value.

[0025] The present invention provides a preparation method of the feed additive according to the above - mentioned technical solution, including: mixing lycopene, capsanthin, pumpkin powder, and zeaxanthin to obtain the feed additive. The present invention does not have special limitations on the mixing method, and it can be evenly mixed by using a conventional mixing method in the art.

[0026] The present invention provides a feed, comprising the feed additive described in the above technical solution or the feed additive prepared by the preparation method described in the above technical solution. As an optional implementation manner of the present invention, the feed further comprises a basal diet. The present invention has no special limitation on the composition of the basal diet, and any basal diet commonly used for poultry feeding in the art can be adopted. As an optional implementation manner of the present invention, the addition amount of the feed additive in the basal diet is 0.1 wt.% to 0.5 wt.%, and can be 0.1 wt.%, 0.2 wt.%, 0.3 wt.%, 0.4 wt.% or 0.5 wt.%.

[0027] In the present invention, the preparation method of the feed comprises: uniformly mixing the diet additive with other components.

[0028] The present invention provides an application of a feed additive or a feed in the breeding of laying poultry. The feed additive is the feed additive described in the above technical solution or the feed additive prepared by the preparation method described in the above technical solution; the feed is the feed described in the above technical solution. As an optional implementation manner of the present invention, the poultry includes chickens. In the present invention, the application includes any one or two or more of the following (1) to (4): (1) Increasing the average daily feed intake of poultry; (2) Increasing the egg production of poultry; (3) Improving the egg quality; (4) Improving the nutritional value of eggs.

[0029] The feed additive or the feed provided by the present invention can increase the average daily feed intake of poultry. The results of the examples of the present invention show that the feed additive can significantly increase the average daily feed intake of laying hens.

[0030] The feed additive or the feed provided by the present invention can increase the egg production of poultry. The results of the examples of the present invention show that the feed additive can significantly increase the weekly egg production of laying hens.

[0031] The feed additive or the feed provided by the present invention can improve the egg quality. As an optional implementation manner of the present invention, the improvement of the egg quality includes any one or two or more of improving the egg weight, yolk weight, egg white weight, eggshell weight, yolk color score, egg white height, eggshell thickness and Haugh unit of the egg. The results of the examples of the present invention show that the feed additive is beneficial to improving the egg weight, yolk weight, egg white weight, eggshell weight, yolk color score, egg white height, eggshell thickness and Haugh unit of eggs, can significantly improve the yolk score, egg white height and Haugh unit, and thus can improve the egg quality.

[0032] The feed additive or the feed provided by the present invention can improve the nutritional value of eggs. As an alternative embodiment of the present invention, the improvement of the nutritional value of eggs includes improving any one or more of vitamin A, polyunsaturated fatty acids, and antioxidant substances in the eggs. The results of the examples of the present invention show that: the feed additive can significantly increase the content of vitamin A and polyunsaturated fatty acids in eggs, and significantly increase the content of antioxidant substances in eggs, etc., thereby being beneficial to improving the nutritional value of eggs.

[0033] The present invention provides a method for improving the egg production rate of poultry and / or improving egg quality, including: Feeding poultry with the feed containing the feed additive described in the above technical solution or the feed additive prepared by the preparation method described in the above technical solution.

[0034] As an alternative embodiment of the present invention, the present invention can add the feed additive described in the above technical solution or the feed additive prepared by the preparation method described in the above technical solution to the basal diet for feeding poultry. In the examples of the present invention, the effect of the feed additive was verified by taking chickens as an example.

[0035] As an alternative embodiment of the present invention, the addition amount of the feed additive in the basal diet can be 0.1wt.% - 0.5wt.%, and can be 0.1wt.%, 0.2wt.%, 0.3wt.%, 0.4wt.% or 0.5wt.%.

[0036] To further illustrate the present invention, the technical solutions provided by the present invention will be described in detail below with reference to the accompanying drawings and examples, but they should not be construed as limiting the protection scope of the present invention.

[0037] The lycopene and capsanthin used in the following examples were purchased from Chenguang Biotech Group Handan Co., Ltd., and the pumpkin powder and zeaxanthin were purchased from Henan Ren'an Biotech Co., Ltd.

[0038] Example 1 A feed additive, by mass, consists of: 20 parts of lycopene, 10 parts of capsanthin, 20 parts of pumpkin powder, and 20 parts of zeaxanthin.

[0039] The preparation method of the feed additive is: Mix lycopene, capsanthin, pumpkin powder, and zeaxanthin by mass to obtain the feed additive.

[0040] Example 2 A feed additive, by mass, consists of: 30 parts of lycopene, 20 parts of capsanthin, 30 parts of pumpkin powder, and 30 parts of zeaxanthin.

[0041] The preparation method of the feed additive is the same as that in Example 1.

[0042] Example 3 A feed additive, by mass, consists of: 25 parts of lycopene, 15 parts of capsanthin, 25 parts of pumpkin powder, and 25 parts of zeaxanthin.

[0043] The preparation method of the feed additive is the same as that in Example 1.

[0044] Example 4 A feed consists of: a basal diet and the feed additive in Example 1, where the addition amount of the feed additive in the basal diet is 0.15 wt.%.

[0045] The basal diet is purchased from Changchun Hefeng Feed Co., Ltd., and its composition is shown in Table 1.

[0046] Table 1 Composition of the basal diet

[0047] Note: 1. The content of the premix in each kilogram of the diet is as follows: vitamin A 8000 IU, vitamin D 3750 IU, vitamin E 100 mg, vitamin B2 12.5 mg, vitamin B6 9 mg, vitamin B12 0.03 mg, pantothenic acid 18 mg, niacin 60 mg, folic acid 1.5 mg, biotin 0.225 mg, iron 80 mg, copper 9 mg, selenium 0.3 mg, manganese 12.25 mg, and zinc 25.2 mg. 2. The values in the table are calculated based on the data provided by the Chinese Feed Database (2013).

[0048] The preparation method of the feed is: mixing the basal diet and the feed additive evenly to obtain the feed.

[0049] Example 5 A feed consists of: a basal diet and the feed additive in Example 1, where the addition amount of the feed additive in the basal diet is 0.25 wt.%. The composition of the basal diet is the same as that in Example 4.

[0050] Example 6 A feed consists of: a basal diet and the feed additive in Example 1, where the addition amount of the feed additive in the basal diet is 0.4 wt.%. The composition of the basal diet is the same as that in Example 4.

[0051] Example 7 A feed consists of: a basal diet and the feed additive in Example 1, where the addition amount of the feed additive in the basal diet is 0.5 wt.%. The composition of the basal diet is the same as that in Example 4.

[0052] Example 8 A feed, composed of: a basal diet and the feed additive in Example 2, wherein the addition amount of the feed additive in the basal diet is 0.2 wt.%. The composition of the basal diet is the same as that in Example 4.

[0053] Example 9 A feed, composed of: a basal diet and the feed additive in Example 2, wherein the addition amount of the feed additive in the basal diet is 0.3 wt.%. The composition of the basal diet is the same as that in Example 4.

[0054] Example 10 A feed, composed of: a basal diet and the feed additive in Example 2, wherein the addition amount of the feed additive in the basal diet is 0.5 wt.%. The composition of the basal diet is the same as that in Example 4.

[0055] Example 11 A feed, composed of: a basal diet and the feed additive in Example 3, wherein the addition amount of the feed additive in the basal diet is 0.1 wt.%. The composition of the basal diet is the same as that in Example 4.

[0056] Example 12 A feed, composed of: a basal diet and the feed additive in Example 3, wherein the addition amount of the feed additive in the basal diet is 0.3 wt.%. The composition of the basal diet is the same as that in Example 4.

[0057] Example 13 A feed, composed of: a basal diet and the feed additive in Example 3, wherein the addition amount of the feed additive in the basal diet is 0.5 wt.%. The composition of the basal diet is the same as that in Example 4.

[0058] Application Example 1 Verification of the feeding effect of the feed containing the feed additive on laying hens 1. Animal selection and experimental design Select 360 185-day-old Jinghong laying hens, randomly divide them into 4 groups, with 90 hens in each group; name them the blank group, experimental group 1, experimental group 2, and experimental group 3 respectively, and each group is randomly divided into 3 sub-groups, with 30 hens in each sub-group; conduct a pre-experiment for 3 days to observe the feed intake, egg production, and health status of the laying hens.

[0059] Among them, the blank group fed the basal diet to each chicken every day. The experimental group 1 fed the feed with 0.15% feed additive added to the basal diet, the experimental group 2 fed the feed with 0.25% feed additive added to the basal diet, and the experimental group 3 fed the feed with 0.4% feed additive added to the basal diet. Each experimental group was fed three times a day, in the morning, at noon, and in the evening, and the amount of feed fed to each chicken each time was 50 g; the remaining feed amount and egg production were recorded every morning, and the average daily feed intake (grams) of each laying hen in each group was calculated, and the weekly egg production (pieces) of each experimental group (30 laying hens) was calculated. Every 7 days, the egg weight, egg yolk weight, egg white weight, egg white height, eggshell thickness, eggshell weight of the eggs produced by the laying hens in each experimental group were detected, and the egg yolk color score was recorded according to the Roche Color Fan. The pictures of the egg yolk color in the eggs from week 1 to week 7 of the experiment are as Figures 1 - 9 shown. On the 49th day of the experiment, 100 eggs were collected from each group for the detection of egg nutritional components and antioxidant indexes in the egg yolk.

[0060] Among them, the basal diet was the basal diet in Example 4, and the feed additive was the feed additive in Example 1.

[0061] 2. Laying Hen Feeding Management It was carried out according to the production management manual of Jinghuadu Group. The large-scale production method was adopted, with step-by-step cage rearing, 3 chickens in each cage, natural ventilation, free drinking water, keeping the pen clean and hygienic, and the immunization program was carried out according to the routine.

[0062] 3. Slaughter and Sample Collection On the 49th day of the experiment, 100 eggs were collected from each experimental group to detect the content of polyunsaturated fatty acids and vitamin A in the eggs and the antioxidant indexes of SOD, T-AOC, DPPH and reducing power in the egg yolk.

[0063] 4. Experimental Data Collection and Result Analysis After the experimental data was preliminarily sorted and statistically analyzed by Excel, the variance analysis was then carried out using the SPSS (29.0) statistical software, and the least significant difference method was used for multiple comparisons; P<0.05 and P<0.01 were used as the judgment criteria for significant and extremely significant differences.

[0064] 5. Experimental Results (1) The detection results of the production performance indexes of laying hens are shown in Table 2.

[0065] Table 2 Detection Results of Laying Hen Production Performance Indexes

[0066] Note: The superscript letters on the right side of the data in the table indicate significant differences between groups (P<0.05), and are marked according to statistical significance.

[0067] As can be seen from Table 2, there were no significant differences in the average daily feed intake, weekly egg production, and feed-to-egg ratio among Experimental Group 1, Experimental Group 2, and Experimental Group 3, which were supplemented with the feed additive two weeks before the experiment; in the third week of the experiment, the average daily feed intake of Experimental Group 1 was significantly higher than that of the blank group, and the weekly egg production of Experimental Groups 1 and 3 was significantly higher than that of the blank group; in the fourth week of the experiment, the average daily feed intake of Experimental Group 2 was significantly higher than that of the blank group, and the weekly egg production of Experimental Groups 1, 2, and 3 was significantly higher than that of the blank group; in the fifth week of the experiment, the weekly egg production of Experimental Groups 1 and 3 was significantly higher than that of the blank group; in the sixth week of the experiment, the average daily feed intake of Experimental Group 1 was significantly increased, and the weekly egg production of Experimental Groups 1 and 3 was significantly higher than that of the blank group; in the seventh week of the experiment, the average daily feed intake of Experimental Groups 1 and 3 was significantly higher than that of the blank group, and the weekly egg production of Experimental Groups 1, 2, and 3 was significantly higher than that of the blank group. The results show that adding the feed additive provided by the present invention to the basic diet of laying hens can effectively increase egg production and daily feed intake.

[0068] (2)The test results of egg quality indexes are shown in Table 3.

[0069] Table 3 Test Results of Egg Quality Indexes

[0070]

[0071]

[0072] Note: The lowercase letters superscripted on the right side of the data in the table indicate significant differences between groups (P<0.05), and the capital letters indicate extremely significant differences between groups (P<0.01), which are marked according to statistical significance.

[0073] As shown in Table 3, in the first week of the experiment, the egg yolk color scores of experimental groups 1, 2, and 3 were significantly higher than those of the blank control group (P<0.01). The albumen height of experimental group 1 was significantly higher than that of the blank group (P<0.05), and the Haugh unit of experimental group 3 was significantly higher than that of the blank control group (P<0.05). In the second week of the experiment, the egg weight and albumen weight of experimental group 1 were significantly higher than those of the blank control group (P<0.05). The eggshell weight of experimental groups 1 and 3 was significantly higher than that of the blank control group (P<0.05). The egg yolk color scores of experimental groups 1, 2, and 3 were significantly higher than those of the blank control group (P<0.01). The albumen height of experimental group 1 was significantly higher than that of the blank group (P<0.05), and the Haugh unit of experimental group 1 was significantly higher than that of the blank group (P<0.05). In the third week of the experiment, the egg weights of experimental groups 1 and 3 were significantly higher than those of the blank group (P<0.05). The egg yolk weight of experimental group 1 was significantly higher than that of the blank group (P<0.05). The albumen weights of experimental groups 1 and 3 were significantly higher than those of the blank group (P<0.05). The egg yolk scores of experimental groups 1, 2, and 3 were significantly higher than those of the blank group (P<0.01). The albumen height of experimental group 1 was significantly higher than that of the blank group (P<0.05), and the eggshell thickness of experimental group 3 was significantly higher than that of the blank control group (P<0.05). In the fourth week of the experiment, the egg weight of experimental group 1 was significantly higher than that of the blank group (P<0.05). The egg yolk weight of experimental group 3 was significantly higher than that of the blank control group (P<0.05). The albumen weights of experimental groups 1 and 3 were significantly higher than those of the blank group (P<0.05). The egg yolk color scores of experimental groups 1, 2, and 3 were significantly higher than those of the blank control group (P<0.01), and the eggshell thickness of experimental group 3 was significantly higher than that of the blank group (P<0.05). In the fifth week of the experiment, the eggshell weights of experimental groups 1 and 3 were significantly higher than those of the blank group (P<0.05). The egg yolk color scores and eggshell thicknesses of experimental groups 1, 2, and 3 were significantly higher than those of the blank group (P<0.01). In the sixth week of the experiment, the egg weights, egg yolk weights, egg yolk color scores, albumen heights, and Haugh units of experimental groups 1, 2, and 3 were significantly higher than those of the blank group (P<0.05). The albumen weight of experimental group 2 was significantly higher than that of the blank group (P<0.05), and the eggshell thickness of experimental group 3 was significantly higher than that of the blank group (P<0.05). In the seventh week of the experiment, the egg yolk color scores, albumen heights, eggshell thicknesses, and Haugh units of experimental groups 1, 2, and 3 were significantly higher than those of the blank group (P<0.05). The egg weights of experimental groups 1 and 3 were significantly higher than those of the blank group. The albumen weights of experimental groups 1 and 2 were significantly higher than those of the blank group (P<0.05), and the egg yolk weight of experimental group 2 was significantly higher than that of the blank group (P<0.05).

[0074] In summary, the feed additive provided by the present invention can significantly improve the egg yolk color score once fed, and the improvement effect is stable. As the feeding time extends to the 6th - 7th week, the feed additive can significantly and stably improve the albumen height and Haugh unit, thus improving the quality of eggs.

[0075] (3)The test results of the nutritional component indicators in eggs are shown in Table 4.

[0076] Table 4 Test Results of Nutritional Component Indicators in Eggs

[0077] It can be analyzed from Table 4 that the contents of vitamin A and polyunsaturated fatty acids in the eggs of experimental group 1 are significantly increased compared with the blank group (P < 0.01); the content of polyunsaturated fatty acids in the eggs of experimental group 2 is significantly increased compared with the blank group (P < 0.01); the contents of vitamin A and polyunsaturated fatty acids in the eggs of experimental group 3 are significantly increased compared with the blank group (P < 0.01). The results show that adding the feed additive provided by the present invention to the basic diet of laying hens can effectively increase the vitamin A and polyunsaturated fatty acids in eggs, and thus increase the content of nutrients in eggs.

[0078] (4)The test results of antioxidant substances in eggs are shown in Table 5.

[0079] Table 5 Test Results of Antioxidant Substances in Eggs

[0080] Superoxide dismutase (SOD) is an important antioxidant enzyme that can scavenge reactive oxygen free radicals and protect cells from oxidative stress damage. A high SOD content in eggs can improve the nutritional value of eggs, and consuming eggs with high SOD activity can improve the antioxidant capacity of the human body. The content of 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging ability reflects the antioxidant capacity of eggs, and a higher DPPH scavenging rate is related to the egg yolk color, freshness and nutritional value. The total antioxidant capacity (T-AOC) content is an important indicator to measure the overall antioxidant performance of eggs. The higher the T-AOC level, the stronger the antioxidant capacity of eggs. The T-AOC content has a positive correlation with the nutritional value, preservation time and light yellow color of eggs. Measuring the reducing power in the egg yolk, the reducing power has a positive correlation with its antioxidant capacity and the potential to resist oxidative damage. The stronger the reducing power, the fresher the eggs, the higher the nutritional value, and the brighter the egg yolk color.

[0081] It can be analyzed from Table 5 that the reducing power, DPPH, SOD and T-AOC contents in the egg yolks of experimental group 1, experimental group 2 and experimental group 3 are significantly increased compared with the blank group (P < 0.01). The results show that adding the feed additive composition can significantly increase the reducing power, superoxide dismutase (SOD), 1,1-diphenyl-2-picrylhydrazyl (DPPH), total antioxidant capacity (T-AOC) contents in the egg yolks. Adding the feed additive provided by the present invention to the basic diet of laying hens can effectively enhance the antioxidant capacity of eggs.

[0082] Comparative Example 1 A feed additive consisting solely of lycopene.

[0083] A feed comprises a basic diet and lycopene, wherein the added amount of lycopene in the basic diet is 0.15%.

[0084] The composition of the basal diet was the same as in Example 4.

[0085] Comparative Example 2 A feed additive consists of lycopene, capsanthin and pumpkin powder, wherein the mass ratio of lycopene, capsanthin and pumpkin powder is 3:2:3.

[0086] A feed comprises a basic diet and the feed additive in this comparative example, wherein the addition amount of the feed additive in the basic diet is 0.15%.

[0087] The composition of the basal diet was the same as in Example 4.

[0088] Comparative Example 3 A feed additive comprises capsanthin, pumpkin powder and zeaxanthin, wherein the mass ratio of capsanthin, pumpkin powder and zeaxanthin is 3:2:3. The addition amount of the feed additive in a basic diet is 0.15%.

[0089] The composition of the basal diet was the same as in Example 4.

[0090] Application Example 2 Feeding effect of the feed provided by Comparative Example 1, Comparative Example 2 and Comparative Example 3 The experiment in Application Example 2 was conducted at the same time as the experiment in Application Example 1, and the animals selected were the same batch of 185-day-old Beijing-red laying hens. The feeding method and laying hen breeding management method were exactly the same. To facilitate the recording of the results, they are listed in the form of Application Example 2.

[0091] 1. Animal Selection and Experimental Design 360 185-day-old Beijing-red laying hens were selected and randomly divided into 4 groups, each with 90 hens; they were named blank group, comparative example group 1, comparative example group 2 and comparative example group 3, and each group was randomly divided into 3 small groups, each with 30 hens; the pre-test lasted 3 days, and the feed intake, egg production and health status of the laying hens were observed. The composition of the basal diet was the same as that of the basal diet in Example 4.

[0092] Among them, the blank group was fed the basal diet every day, the control group 1 was fed the feed of Comparative Example 1 every day, the control group 2 was fed the feed of Comparative Example 2 every day, and the control group 3 was fed the feed of Comparative Example 3 every day. The feed of the experimental group or the basal diet of the blank group was fed three times a day, in the morning, at noon and in the evening; the remaining feed amount and egg production were recorded every morning, and the average daily feed intake (grams) of each laying hen was calculated, and the weekly egg production (pieces) of each experimental group (30 laying hens) was calculated. Every 7 days, the egg weight, egg yolk weight, egg white weight, egg white height, eggshell thickness, eggshell weight, etc. of the eggs produced by the laying hens in each experimental group were detected. On the 42nd day of the experiment, 100 eggs were collected from each group for the detection of egg nutritional components and antioxidant indexes in the egg yolk.

[0093] 2. Laying Hen Feeding Management It was carried out according to the production management manual of Jinghuadu Group. The large-scale production method was adopted, with stepped cage farming, 3 hens in each cage, natural ventilation, free drinking water, keeping the pens clean and hygienic, and the immunization program was carried out routinely.

[0094] 3. Slaughtering and Sample Collection On the 49th day of the experiment, 100 eggs were collected from each experimental group to detect the content of polyunsaturated fatty acids and vitamin A in the eggs, as well as the antioxidant indexes of SOD, T-AOC, DPPH and reducing power in the egg yolk.

[0095] 4. Experimental Data Collection and Result Analysis After the experimental data was preliminarily sorted and statistically analyzed by Excel, the variance analysis was then carried out using the SPSS (29.0) statistical software, and the least significant difference method was used for multiple comparisons; P<0.05 and P<0.01 were used as the judgment criteria for significant and extremely significant differences.

[0096] 5. Experimental Results (1) The detection results of the production performance of laying hens are shown in Table 7.

[0097] Table 7 Detection Results of Laying Hen Production Performance

[0098] It can be seen from Table 7 that on the 21st day of the experiment, the average daily feed intakes of the Comparative Example 2 and 3 groups were significantly increased compared with the blank group and the control group 1 (P<0.05). On the 28th day of the experiment, the average daily feed intake of the Comparative Example 2 group was significantly increased compared with the blank group (P<0.05). On the 35th day of the experiment, the weekly egg production of the Comparative Example 2 group was significantly increased compared with the blank group (P<0.05).

[0099] (2) The detection results of the nutritional component indexes in the eggs are shown in Table 8.

[0100] Table 8 Detection Results of Nutritional Component Indexes in Eggs

[0101] As shown in Table 8, the vitamin A content in the control group 2 was significantly higher than that in the blank group (P<0.01), and the polyunsaturated fatty acid content in the control groups 1 and 3 was significantly higher than that in the blank group (P<0.01). Adding lycopene to the basal diet significantly increased the vitamin A content in eggs compared with adding only the basal diet. There was no significant change in improving vitamin A in eggs in the feeding group of the feed additive obtained by combining lycopene, capsanthin and pumpkin powder compared with the blank group. Adding lycopene or the feed additive composed of lycopene, capsanthin and pumpkin powder to the basal diet significantly increased the polyunsaturated fatty acid content in eggs compared with the blank group.

[0102] (3)The detection results of antioxidant substances in eggs are shown in Table 9.

[0103] Table 9 Detection Results of Antioxidant Substances in Eggs

[0104] As shown in Table 9, compared with the blank group, the reducing power in the control group 3 was significantly higher than that in the blank group (P<0.05), the DPPH content in the control groups 1, 2 and 3 was significantly higher than that in the blank group (P<0.01), and the SOD and T-AOC contents in the control group 1 were significantly higher than that in the blank group (P<0.05). The T-AOC content in the control groups 1, 2 and 3 was significantly higher than that in the blank group (P<0.01).

[0105] (4)The detection results of egg quality are shown in Tables 10 - 15.

[0106] Table 10 Detection Results of Egg Quality in Each Test Group in the First Week

[0107] Table 11 Detection Results of Egg Quality in Each Test Group in the Second Week

[0108] Table 12 Detection Results of Egg Quality in Each Test Group in the Third Week

[0109] Table 13 Detection Results of Egg Quality in Each Test Group in the Fourth Week

[0110] Table 14 Detection Results of Egg Quality in Each Test Group in the Fifth Week

[0111] Table 15 Detection Results of Egg Quality in Each Test Group in the Sixth Week

[0112] In summary, the feed additive provided by the present invention can significantly increase the average daily feed intake of laying hens, increase the egg production, improve the egg quality and increase the nutritional value of eggs.

[0113] Although the above embodiments have described the present invention in detail, they are only a part of the embodiments of the present invention, rather than all embodiments. People can also obtain other embodiments based on these embodiments without creative efforts, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A feed additive, characterized in that, The feed additive includes, by mass parts: 20 - 30 parts of lycopene, 10 - 20 parts of capsanthin, 20 - 30 parts of pumpkin powder, and 20 - 30 parts of zeaxanthin.

2. The preparation method of the feed additive according to claim 1, characterized in that, It includes: Mix lycopene, capsanthin, pumpkin powder, and zeaxanthin to obtain the feed additive.

3. A feed, characterized in that, It includes the feed additive described in claim 1 or the feed additive prepared by the preparation method described in claim 2.

4. The feed according to claim 3, wherein The feed further includes a basal diet.

5. The feed according to claim 4, characterized in that, The addition amount of the feed additive in the basal diet is 0.1wt.% - 0.5wt.%.

6. An application of a feed additive or a feed in the breeding of laying poultry, wherein the feed additive is the feed additive described in claim 1 or the feed additive prepared by the preparation method described in claim 2; the feed is the feed described in any one of claims 3 - 5.

7. The application according to claim 6, characterized in that The application includes any one or more than two of the following (1) - (4): (1) Improve the average daily feed intake of poultry; (2) Increase the egg production of poultry; (3) Improve the quality of eggs; (4) Improve the nutritional value of eggs.

8. The application according to claim 6 or 7, characterized in that The poultry includes chickens.

9. A method for improving the egg production rate of poultry and / or improving egg quality, characterized in that, It includes: Feed poultry with the feed containing the feed additive described in claim 1 or the feed additive prepared by the preparation method described in claim 2.

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