Antibacterial composition and application thereof in hand sanitizer

Abstract

The invention discloses a bacteriostatic composition and an application thereof in a hand sanitizer, the bacteriostatic composition comprises the following components by mass: 3-5% of lysozyme, 4-8% of an enzyme protection system, 8-12% of a plant bacteriostatic extract, 10-15% of a biological matrix carrier, 5-8% of a stable protection system, and the balance of water. According to the antibacterial composition, a three-dimensional network structure is formed through the carboxymethyl chitosan-sodium alginate compound, a protective layer with good biocompatibility can be formed on the skin surface, and the skin retention time of lysozyme and plant antibacterial active ingredients is remarkably prolonged. According to the invention, lysozyme and plant antibacterial components are adopted to form multiple antibacterial mechanisms: the lysozyme specifically hydrolyzes peptidoglycan in bacterial cell walls, and active components in the plant antibacterial extract destroy bacterial cell membranes and inhibit bacterial metabolism. The double-attack mechanism has a stronger antibacterial effect than a single component, and can prevent bacteria from generating drug resistance at the same time.

Description

Technical Field

[0001] The present invention relates to the technical field of hand sanitizers, and specifically relates to an antibacterial composition and its application in hand sanitizers. Background Art

[0002] With the continuous improvement of people's awareness of personal hygiene, hand sanitizers, as a convenient and effective hand cleaning and antibacterial product, are widely used. Currently, hand sanitizer products on the market are mainly divided into two categories: ordinary cleaning type and antibacterial type. Antibacterial hand sanitizers usually use chemically synthesized antibacterial agents as the main active ingredients, such as triclosan, quaternary ammonium salt compounds (such as benzalkonium bromide), etc. Although these chemical antibacterial components have strong immediate antibacterial effects, they have the following obvious deficiencies: First, most of the chemical antibacterial agents are washed away with water after handwashing, and the residual amount is small, resulting in poor antibacterial persistence; Second, long-term use may cause adverse reactions such as skin dryness and allergies; Third, some chemical antibacterial agents such as triclosan have been proven to possibly disrupt the endocrine system and promote the generation of drug-resistant strains, and have been restricted or prohibited in many countries and regions; Finally, these compounds have poor biodegradability and pose potential hazards to the environment.

[0003] In recent years, the application of natural antibacterial components such as plant extracts and enzymes such as lysozyme in antibacterial products has gradually increased. Components such as polyphenols, flavonoids, and terpenoids in plant extracts have broad-spectrum antibacterial activities, and are gentle to the human body and friendly to the environment. Lysozyme, as a hydrolase widely present in nature, can specifically hydrolyze the peptidoglycan component in the bacterial cell wall, destroy the cell structure and cause bacteria to die, and at the same time has the advantages of high safety and low tendency to produce drug resistance.

[0004] However, hand sanitizers based on natural antibacterial components in the prior art still face many technical problems: Lysozyme, as a protein enzyme, has poor stability in the hand sanitizer formulation environment, and its activity is easily affected by pH, temperature, and chemical substances and decreases; The contact time between natural antibacterial components and the skin is short, and it is difficult to form an effective and lasting antibacterial effect; The antibacterial spectrum of a single antibacterial component is limited and it is difficult to cover a variety of common pathogenic bacteria.

[0005] The currently published patent document CN113967251A discloses an antibacterial agent containing lysozyme, which is composed of plant extracts, lysozyme, pine oil, benzalkonium bromide, and a penetration aid. This technology forms a film on the skin surface through pine oil to extend the residence time of each antibacterial active substance on the skin, thereby achieving a better lasting antibacterial effect. However, this technology still has obvious deficiencies: It still relies on chemical antibacterial agents such as benzalkonium bromide; It does not provide an effective solution to the stability problem of lysozyme; The film formed by pine oil may bring an uncomfortable greasy feeling to users.

[0006] Therefore, how to develop an antibacterial composition that does not contain chemical antibacterial agents, has a long-lasting antibacterial effect, and can effectively protect the activity of lysozyme has become an important research and development direction in the current hand sanitizer technology field. Summary of the Invention

[0007] Based on the problems existing in the background technology, the present invention provides an innovative antibacterial composition based on lysozyme and natural plant extracts and its application in hand sanitizers.

[0008] The present invention is implemented through the following technical solutions:

[0009] In the first aspect of the present invention, an antibacterial composition is disclosed, which includes the following components by mass percentage: 3-5% of lysozyme, 4-8% of an enzyme protection system, 8-12% of a plant antibacterial extract, 10-15% of a biological matrix carrier, 5-8% of a stability protection system, and the balance is water.

[0010] Further, the enzyme protection system includes cofactors, enzyme stabilizers, and antioxidant protectants;

[0011] The mass ratio of the cofactors, enzyme stabilizers, and antioxidant protectants is (0.3-0.5):(3-6):(0.3-0.5);

[0012] Among them, the cofactors are magnesium ions and zinc ions, and the molar ratio of magnesium ions to zinc ions is 2:1;

[0013] The enzyme stabilizers are mannitol and trehalose, and the mass ratio of mannitol to trehalose is (1-2.5):1;

[0014] The antioxidant protectant is a mixture of glutathione and disodium EDTA, and the mass ratio is 1:0.2.

[0015] In the present invention, a multiple protection system for lysozyme is designed, including structural stability protection, antioxidant protection, and microenvironment regulation, so that lysozyme maintains high activity in a complex formulation environment. Mannitol and trehalose, as osmoprotectants, can stabilize the tertiary structure of lysozyme; glutathione and vitamin E acetate form an antioxidant network to inhibit protein oxidation; while the cofactors (magnesium ions, zinc ions) maintain the catalytic activity of the enzyme.

[0016] Further, the plant antibacterial extract includes green tea extract, pomegranate peel extract, grape seed extract, rosemary extract, and lemon extract;

[0017] The mass ratio of the green tea extract, pomegranate peel extract, grape seed extract, rosemary extract, and lemon extract is (2-3):(1.5-2.5):(1.5-2.5):(1.5-2.5):(1-2).

[0018] Further, the biological matrix carrier includes carboxymethyl chitosan-sodium alginate complex, hyaluronic acid, glycerol, and betaine;

[0019] The mass ratio of carboxymethyl chitosan-sodium alginate complex, hyaluronic acid, glycerol, and betaine is (4 - 8):(1 - 2):(2 - 3):(2 - 4).

[0020] Further, the stability protection system includes a fat-soluble antioxidant, panthenol, a pH buffer solution, and an emulsifier;

[0021] The mass ratio of the fat-soluble antioxidant, panthenol, the pH buffer system, and the emulsifier is (1.5 - 2.5):(1 - 2):(2 - 3):(0.2 - 0.8);

[0022] Among them, the fat-soluble antioxidant is vitamin E acetate; the pH buffer solution is a lactic acid / sodium lactate buffer solution.

[0023] Further, the pH value of the antibacterial composition is 6.5 - 7.0.

[0024] The second aspect of the present invention discloses a method for the antibacterial composition, including the following steps:

[0025] (1) Prepare the biological matrix carrier: Dissolve carboxymethyl chitosan in water, dissolve sodium alginate in water, slowly add the sodium alginate solution to the carboxymethyl chitosan solution, after mixing evenly, successively add hyaluronic acid, betaine, and glycerol, and mix evenly to obtain the biological matrix carrier;

[0026] (2) Prepare the lysozyme stabilization system: Dissolve the enzyme stabilizer in a phosphate buffer solution at pH 6.3, adjust the temperature to 20 ± 2 °C, add lysozyme, cofactors, and antioxidant protectants, and stir at 50 - 100 rpm until uniform to obtain the lysozyme stabilization system;

[0027] (3) Prepare the plant antibacterial extract stabilization system: Add an emulsifier and a fat-soluble antioxidant to an ethanol-water mixed solution, after dissolution, successively add the plant antibacterial extract components, mix evenly and then concentrate under reduced pressure to remove ethanol to obtain the plant antibacterial extract stabilization system;

[0028] (4) Add the lysozyme stabilization system obtained in step (2) to the biological matrix carrier obtained in step (1), and stir evenly;

[0029] (5) Add the plant antibacterial extract stabilization system obtained in step (3) to the mixture obtained in step (4), stir evenly, add panthenol and a pH buffer solution, adjust the pH value, homogenize and emulsify, and then fill to obtain the final antibacterial composition.

[0030] The third aspect of the present invention discloses the application of the antibacterial composition in hand sanitizer, and the hand sanitizer comprises the following components in percentage by weight: antibacterial composition 1-3%, dodecyl glucoside 2.5-3.5%, betaine 1.5-2.5%, glycerol 2-3%, panthenol 0.5-1%, thickener 0.5-0.8%, lactic acid / sodium lactate buffer solution 0.2-0.4%, preservative 0.3-0.5%, essence 0.05-0.1%, and the balance is water.

[0031] Further, the thickener is xanthan gum; the preservative is benzyl alcohol and / or phenoxyethanol.

[0032] Further, the pH value of the hand sanitizer is 6.5-7.0.

[0033] Advantages of the present invention:

[0034] 1. The antibacterial composition of the present invention can form a protective layer with good biocompatibility on the skin surface through the three-dimensional network structure formed by the carboxymethyl chitosan-sodium alginate complex, and can significantly prolong the skin retention time of lysozyme and plant antibacterial active ingredients. Different from the rapid decline of antibacterial activity after rinsing with traditional hand sanitizers, the hand sanitizer of the present invention still maintains an antibacterial rate of more than 85% within 8 hours after use. In the present invention, a multiple antibacterial mechanism is formed by lysozyme and plant antibacterial components: lysozyme specifically hydrolyzes peptidoglycan in the bacterial cell wall, while plant active ingredients such as green tea extract, pomegranate peel extract, and grape seed extract destroy the bacterial cell membrane and inhibit bacterial metabolism. This dual attack mechanism has a stronger antibacterial effect than single components and can prevent bacteria from developing drug resistance.

[0035] 2. The components of the antibacterial composition of the present invention are all derived from natural substances or materials with good biocompatibility, the skin irritation index <0.5, and it has a moisturizing and skin care effect. Long-term use will not cause skin dryness and irritation, solving the adverse effects of traditional antibacterial hand sanitizers on the skin. In the present invention, chemical antibacterial agents such as triclosan and benzalkonium bromide are completely removed, and all components have good biodegradability, are environmentally friendly, and conform to the concept of green sustainable development. Specific embodiments

[0036] The technical solutions of the present invention will be further described in detail below with reference to specific embodiments, but the protection scope of the present invention is not limited to the following embodiments.

[0037] Preparation examples of antibacterial agents

[0038] Preparation example 1

[0039] An antibacterial composition, by mass percentage, comprises the following components:

[0040] Lysozyme 4.0%

[0041] Enzyme protection system 6.0%: Cofactors (magnesium sulfate, zinc sulfate, molar ratio 2:1) 0.38%; Enzyme stabilizers (mannitol, trehalose, mass ratio 2:1) 5.0%; Antioxidant protectants (glutathione, disodium EDTA, mass ratio 1:0.2) 0.42%;

[0042] Plant antibacterial extract 10.0%: Green tea extract 2.5%, pomegranate peel extract 2.0%, grape seed extract 2.0%, rosemary extract 2.0%, lemon extract 1.5%;

[0043] Biological matrix carrier 12.0%: Carboxymethyl chitosan - sodium alginate complex 6.0%, hyaluronic acid 1.2%, glycerol 2.3%, betaine 2.5%;

[0044] Stable protection system 6.2%: Vitamin E acetate 1.8%, panthenol 1.5%, lactic acid / sodium lactate buffer solution 2.5%, emulsifier (polysorbate 80) 0.4%;

[0045] Purified water to make up the balance to 100%.

[0046] Method for antibacterial composition, comprising the following steps:

[0047] (1) Preparation of biological matrix carrier: Dissolve carboxymethyl chitosan (4.5%) in water, dissolve sodium alginate (1.5%) in water, slowly add the sodium alginate solution to the carboxymethyl chitosan solution, after mixing evenly, successively add hyaluronic acid, betaine and glycerol, stir at 500 rpm for 40 minutes to mix evenly, to obtain the biological matrix carrier;

[0048] (2) Preparation of lysozyme stabilization system: Dissolve mannitol and trehalose in phosphate buffer at pH 6.3, adjust the temperature to 20 ± 2 °C, add lysozyme, magnesium sulfate, zinc sulfate, glutathione and disodium EDTA, stir at 80 rpm for 60 minutes until uniform, to obtain the lysozyme stabilization system;

[0049] (3) Preparation of plant antibacterial extract stabilization system: Add polysorbate 80 and vitamin E acetate to ethanol - water (7:3) mixed solution, after dissolution, successively add green tea extract, pomegranate peel extract, grape seed extract, rosemary extract and lemon extract, mix evenly in a 40 °C water bath and then concentrate under reduced pressure at 45 °C to remove ethanol, to obtain the plant antibacterial extract stabilization system;

[0050] (4) Add the lysozyme stabilization system obtained in step (2) to the biological matrix carrier obtained in step (1), stir at 400 rpm for 30 minutes until uniform;

[0051] (5) Add the plant antibacterial extract stabilization system obtained in step (3) to the mixture obtained in step (4), stir evenly, add panthenol and lactic acid / sodium lactate buffer solution, adjust the pH value to 6.8, homogenize at 6000 rpm for 5 minutes, and then fill into containers to obtain the final antibacterial composition.

[0052] Preparation Example 2

[0053] An antibacterial composition, by mass percentage, comprises the following components:

[0054] Lysozyme 4.5%

[0055] Enzyme protection system 6.92%: Cofactor (magnesium sulfate, zinc sulfate, molar ratio 2:1) 0.44%; Enzyme stabilizer (mannitol, trehalose, mass ratio 2:1) 6.0%; Antioxidant protection agent (glutathione, disodium EDTA, mass ratio 1:0.2) 0.48%;

[0056] Plant antibacterial extract 11.0%: Green tea extract 2.8%, pomegranate peel extract 2.2%, grape seed extract 2.2%, rosemary extract 2.2%, lemon extract 1.6%;

[0057] Biological matrix carrier 14.5%: Carboxymethyl chitosan-sodium alginate complex 7.0% (carboxymethyl chitosan 5.0%, sodium alginate 2.0%), hyaluronic acid 1.8%, glycerol 2.5%, betaine 3.2%;

[0058] Stable protection system 7.1%: Vitamin E acetate 2.0%, panthenol 1.8%, lactic acid / sodium lactate buffer solution 2.8%, emulsifier (polysorbate 80) 0.5%;

[0059] Purified water up to 100%.

[0060] The preparation method of the antibacterial agent is the same as that of Preparation Example 1.

[0061] Comparative Preparation Example 1

[0062] Based on Preparation Example 1, this comparative preparation example does not include the enzyme protection system, and the rest are the same as Preparation Example 1;

[0063] The preparation method of the antibacterial composition is specifically as follows:

[0064] (1) Prepare the biological matrix carrier: same as Preparation Example 1;

[0065] (2) Prepare the lysozyme solution: directly dissolve lysozyme in 200 mL of phosphate buffer solution with a pH of 6.3 without adding any components of the enzyme protection system;

[0066] (3) Prepare the plant antibacterial extract stabilization system: same as Preparation Example 1;

[0067] (4) Add the lysozyme solution obtained in step (2) to the biological matrix carrier obtained in step (1), and stir at 400 rpm for 30 minutes until uniform.

[0068] (5) Add the plant antibacterial extract stabilization system obtained in step (3) to the mixture obtained in step (4), stir evenly, and perform subsequent operations in the same manner as in Preparation Example 1 to obtain the final antibacterial composition.

[0069] Comparative Preparation Example 2

[0070] Based on Preparation Example 1, this comparative preparation example lacks the plant antibacterial extract.

[0071] The preparation method of the antibacterial composition is specifically as follows:

[0072] (1) Prepare the biological matrix carrier: the same as Preparation Example 1.

[0073] (2) Prepare the lysozyme stabilization system: the same as Preparation Example 1.

[0074] (3) Add the lysozyme stabilization system to the biological matrix carrier and stir evenly.

[0075] (4) Add vitamin E acetate and an emulsifier to the mixture obtained in step (3), stir evenly, add panthenol and lactic acid / sodium lactate buffer solution, adjust the pH value to 6.8, and perform subsequent operations in the same manner as in Preparation Example 1 to obtain the final antibacterial composition.

[0076] Comparative Preparation Example 3

[0077] Based on Preparation Example 1, this comparative preparation example uses hydroxypropyl methylcellulose as the carrier.

[0078] The preparation method of the antibacterial composition is specifically as follows:

[0079] (1) Prepare the biological matrix carrier: Dissolve 1.5% of hydroxypropyl methylcellulose (HPMC) in water, add 3.0% of glycerol, and stir evenly to obtain the carrier.

[0080] (2) Prepare the lysozyme stabilization system: the same as Preparation Example 1.

[0081] (3) Prepare the plant antibacterial extract stabilization system: the same as Preparation Example 1.

[0082] (4) Add the lysozyme stabilization system obtained in step (2) to the carrier obtained in step (1), and stir at 400 rpm for 30 minutes until uniform.

[0083] (5) Add the stabilization system of the plant antibacterial extract obtained in step (3) to the mixture obtained in step (4), stir evenly, and perform subsequent operations in the same manner as in Preparation Example 1 to obtain the final antibacterial composition.

[0084] Comparative Preparation Example 4

[0085] On the basis of Preparation Example 1, the types of plant antibacterial extracts only include green tea extract and pomegranate peel extract, and grape seed extract, rosemary extract and lemon extract are omitted;

[0086] The preparation method of the antibacterial composition is the same as that of Preparation Example 1.

[0087] Performance test of antibacterial composition

[0088] 1. Antibacterial activity test

[0089] The antibacterial effects of the antibacterial composition on Staphylococcus aureus, Escherichia coli, Candida albicans and methicillin-resistant Staphylococcus aureus (MRSA) were determined by the plate diffusion method. Each test strain was inoculated on a suitable culture medium plate, holes (6 mm in diameter) were punched on the plate, and 100 μL of the antibacterial composition of different preparation examples was added to each hole. After culturing at 37 °C for 24 hours, the diameter of the antibacterial zone was measured. The specific results are shown in Table 1.

[0090] Table 1 Results of antibacterial activity test (diameter of antibacterial zone, mm)

[0091] Group Staphylococcus aureus Escherichia coli Candida albicans MRSA Preparation Example 1 18.5 16.8 15.4 16.2 Preparation Example 2 19.2 17.5 16.0 16.9 Comparative Preparation Example 1 12.6 11.5 10.2 9.8 Comparative Preparation Example 2 14.8 13.2 9.5 12.3 Comparative Preparation Example 3 15.9 14.7 13.5 13.5 Comparative Preparation Example 4 16.3 15.1 12.2 13.8

[0092] It can be seen from the results in Table 1 that Preparation Example 1 and Preparation Example 2 showed significantly better antibacterial effects than the comparative examples. The antibacterial activity of Comparative Preparation Example 1 (lacking the enzyme protection system) was significantly reduced, indicating that the enzyme protection system is crucial for maintaining the activity of lysozyme; the inhibitory effect on fungi of Comparative Preparation Example 2 (lacking plant antibacterial extract) was significantly weakened, indicating that plant antibacterial extracts play an important role in combating fungal infections; the antibacterial effect of Comparative Example 3 (using a common carrier) was between the preparation examples and other comparative examples, indicating that the biological matrix carrier can improve antibacterial activity. Although Comparative Preparation Example 4 (incomplete types of plant antibacterial extracts) had a certain inhibitory effect on common strains, its antibacterial effects on Candida albicans and MRSA were significantly inferior to the complete formula, indicating that the synergistic effect of multiple plant extracts is crucial for expanding the antibacterial spectrum and enhancing antibacterial efficacy.

[0093] 2. Lysozyme activity stability test

[0094] Store the antibacterial compositions of each preparation example at 40 °C for 0 days, 30 days, 60 days, 90 days, and 180 days, and measure the residual activity of lysozyme. Taking the initial activity as 100%, calculate the relative activity percentage at each time point. The specific results are shown in Table 2.

[0095] Table 2 Results of Lysozyme Activity Stability Test (Residual Activity %, Storage Condition: 40°C)

[0096] Group 0 days 30 days 60 days 90 days 180 days Preparation Example 1 100 92.5 85.3 78.6 70.3 Preparation Example 2 100 93.8 86.9 80.2 72.5 Comparative Preparation Example 1 100 65.3 42.6 25.8 12.4 Comparative Preparation Example 2 100 90.2 82.5 75.4 65.8 Comparative Preparation Example 3 100 83.7 70.4 58.9 45.2 Comparative Preparation Example 4 100 88.6 79.5 71.8 62.3

[0097] As can be seen from the results in Table 2, Preparation Example 1 and Preparation Example 2 showed excellent lysozyme activity retention rates under high-temperature storage conditions, with approximately 80% activity remaining after 90 days and more than 70% activity remaining after 180 days. In Comparative Example 1, due to the lack of an enzyme protection system, the lysozyme activity decreased rapidly, and only 12.4% remained after 180 days; in Comparative Example 3, a common carrier was used instead of the biological matrix carrier, resulting in a significant reduction in lysozyme stability, indicating that the biological matrix carrier and the enzyme protection system of the present invention act synergistically to effectively protect the lysozyme activity.

[0098] Example 1

[0099] A hand sanitizer containing an antibacterial composition, the hand sanitizer comprising the following components in weight percentages: antibacterial composition 2.5%, dodecyl glucoside 3.0%, betaine 2.0%, glycerol 2.5%, panthenol 0.7%, xanthan gum 0.6%, lactic acid / sodium lactate buffer solution 0.28%, benzyl alcohol 0.2, phenoxyethanol 0.25%, fragrance 0.08%, and the balance being water.

[0100] The antibacterial composition used in this example is the antibacterial composition prepared in Preparation Example 1.

[0101] The preparation method of the hand sanitizer includes the following steps:

[0102] 1. Slowly add xanthan gum to a part of purified water, and stir at 600 rpm until completely dissolved to obtain a uniform thickening system;

[0103] 2. Add dodecyl glucoside, betaine, glycerol, and panthenol to another part of water, heat to 45°C, and stir until completely dissolved to obtain a surfactant system;

[0104] 3. Slowly add the surfactant system to the thickening system, stir at 400 rpm for 10 minutes until well mixed; add the antibacterial composition, stir at 500 rpm for 15 minutes until uniformly dispersed; add the lactic acid / sodium lactate buffer solution, adjust the pH to 6.8; add benzyl alcohol and phenoxyethanol as preservatives, and stir well; finally add the fragrance and stir well; homogenize at 5000 rpm for 3 minutes, filter through a 0.45 μm filter membrane, and fill.

[0105] Example 2

[0106] The antibacterial composition used in this example is the antibacterial composition prepared in Preparation Example 2; the remaining components and the preparation method of the hand sanitizer are the same as those in Example 1.

[0107] Comparative Example 1

[0108] The antibacterial composition used in this comparative example was the antibacterial composition prepared in Comparative Preparation Example 1; the remaining components and the preparation method of the hand sanitizer were the same as those in Example 1.

[0109] Comparative Example 2

[0110] The antibacterial composition used in this comparative example was the antibacterial composition prepared in Comparative Preparation Example 2; the remaining components and the preparation method of the hand sanitizer were the same as those in Example 1.

[0111] Comparative Example 3

[0112] The antibacterial composition used in this comparative example was the antibacterial composition prepared in Comparative Preparation Example 3; the remaining components and the preparation method of the hand sanitizer were the same as those in Example 1.

[0113] Comparative Example 4

[0114] The antibacterial composition used in this comparative example was the antibacterial composition prepared in Comparative Preparation Example 4; the remaining components and the preparation method of the hand sanitizer were the same as those in Example 1.

[0115] Test Example

[0116] Thirty volunteers were recruited. Each person washed their right hand with the test sample and their left hand with ordinary soap as a control. Hand microbial samples were collected at 0 hour, 2 hours, 4 hours, and 8 hours after handwashing, and the antibacterial rate was calculated. The test results are shown in Table 3.

[0117] Table 3 Antibacterial Persistence after Handwashing with Hand Sanitizer (%)

[0118]

[0119]

[0120] It can be seen from the test results in Table 3 that the hand sanitizers of Example 1 and Example 2 still maintained an antibacterial rate of over 85% within 8 hours after use, significantly superior to each comparative example and the ordinary soap control group. In Comparative Example 1, due to the lack of an enzyme protection system, the activity of lysozyme was unstable, resulting in a significant decrease in antibacterial persistence, especially a rapid decline in the antibacterial rate after 4 hours and 8 hours; in Comparative Example 2, the lack of plant antibacterial extracts led to a narrow antibacterial spectrum and less persistence than the complete formula; in Comparative Example 3, using an ordinary carrier instead of a biological matrix carrier reduced the retention time of the antibacterial components on the skin, affecting the long-term antibacterial effect; in Comparative Example 4, although it contained some plant antibacterial extracts, due to incomplete components, the antibacterial persistence was lower than that of Example 1 and Example 2.

[0121] Finally, it should be noted that the above-described embodiments only represent several implementation manners of the present invention, and are not intended to limit the present invention. For those of ordinary skill in the art, any modifications, equivalent replacements, improvements, etc. made without departing from the concept of the present invention shall be included within the protection scope of the present invention. Therefore, the protection scope of this invention patent shall be subject to the appended claims.

Claims

1. An antibacterial composition, characterized in that By mass percentage, it comprises the following components: lysozyme 3 - 5%, enzyme protection system 4 - 8%, plant antibacterial extract 8 - 12%, biological matrix carrier 10 - 15%, stable protection system 5 - 8%, and the balance is water.

2. The antibacterial composition according to claim 1, wherein The enzyme protection system includes cofactors, enzyme stabilizers, and antioxidant protectants; The mass ratio of cofactors, enzyme stabilizers, and antioxidant protectants is (0.3 - 0.5):(3 - 6):(0.3 - 0.5); Among them, the cofactors are magnesium ions and zinc ions, and the molar ratio of magnesium ions to zinc ions is 2:1; The enzyme stabilizers are mannitol and trehalose, and the mass ratio of mannitol to trehalose is (1 - 2.5):1; The antioxidant protectant is a mixture of glutathione and disodium EDTA, and the mass ratio is 1:0.

2.

3. The antibacterial composition according to claim 1, wherein The plant antibacterial extract includes green tea extract, pomegranate peel extract, grape seed extract, rosemary extract, and lemon extract; The mass ratio of green tea extract, pomegranate peel extract, grape seed extract, rosemary extract, and lemon extract is (2 - 3):(1.5 - 2.5):(1.5 - 2.5):(1.5 - 2.5):(1 - 2).

4. The antibacterial composition according to claim 1, wherein The biological matrix carrier includes carboxymethyl chitosan - sodium alginate complex, hyaluronic acid, glycerol, and betaine; The mass ratio of carboxymethyl chitosan - sodium alginate complex, hyaluronic acid, glycerol, and betaine is (4 - 8):(1 - 2):(2 - 3):(2 - 4).

5. The antibacterial composition according to claim 1, wherein The stable protection system includes lipophilic antioxidants, panthenol, pH buffer solution, and emulsifiers; The mass ratio of lipophilic antioxidants, panthenol, pH buffer system, and emulsifiers is (1.5 - 2.5):(1 - 2):(2 - 3):(0.2 - 0.8); Among them, the lipophilic antioxidant is vitamin E acetate; the pH buffer solution is lactic acid / sodium lactate buffer solution.

6. The antibacterial composition according to any one of claims 1-5, characterized in that, The pH value of the antibacterial composition is 6.5 - 7.

0.

7. A method for preparing the antibacterial composition according to any one of claims 1-6, characterized in that, It includes the following steps: (1) Prepare the biological matrix carrier: Dissolve carboxymethyl chitosan in water, dissolve sodium alginate in water, slowly add the sodium alginate solution to the carboxymethyl chitosan solution, after mixing evenly, successively add hyaluronic acid, betaine, and glycerol, and mix evenly to obtain the biological matrix carrier; (2) Prepare the lysozyme stabilization system: Dissolve the enzyme stabilizer in a phosphate buffer solution with a pH of 6.3, adjust the temperature to 20 ± 2 °C, add lysozyme, cofactors, and antioxidant protectants, and stir at 50 - 100 rpm until uniform to obtain the lysozyme stabilization system; (3) Prepare the plant antibacterial extract stabilization system: Add emulsifiers and lipophilic antioxidants to an ethanol - water mixed solution, after dissolution, successively add the components of the plant antibacterial extract, mix evenly, and then concentrate under reduced pressure to remove ethanol to obtain the plant antibacterial extract stabilization system; (4) Add the lysozyme stabilization system obtained in step (2) to the biological matrix carrier obtained in step (1), and stir evenly; (5) Add the plant antibacterial extract stabilization system obtained in step (3) to the mixture obtained in step (4), stir evenly, add panthenol and a pH buffer solution, adjust the pH value, and fill after homogenization and emulsification to obtain the final antibacterial composition.

8. Use of the antibacterial composition according to any one of claims 1-6 in a hand sanitizer, characterized in that, The hand sanitizer contains the following components in weight percentages: 1-3% of the antibacterial composition, 2.5-3.5% of dodecyl glucoside, 1.5-2.5% of betaine, 2-3% of glycerol, 0.5-1% of panthenol, 0.5-0.8% of thickener, 0.2-0.4% of lactic acid / sodium lactate buffer solution, 0.3-0.5% of preservative, 0.05-0.1% of essence, and the balance is water.

9. The application according to claim 8, wherein The thickener is xanthan gum; the preservative is benzyl alcohol and / or phenoxyethanol.

10. The application according to claim 8, wherein The pH value of the hand sanitizer is 6.5-7.0.

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