Composition containing alpine plant extract and ergothioneine and application

Abstract

The invention provides a composition containing alpine plant extracts and ergothioneine. The composition is prepared from the following components in parts by weight: 0.005 to 0.8 part of crocus sativus extracts, 0.01 to 0.03 part of alpine radix scutellariae extracts, 0.1 to 0.4 part of alpine leontopodium extracts, 0.0005 to 0.005 part of alpine rose rhododendron extracts and 0.001 to 0.15 part of ergothioneine. The invention further provides a skin care product containing the composition containing the alpine plant extract and the ergothioneine. The composition and the skin care product containing the composition have excellent skin care effects of resisting light aging, relieving and resisting oxidation.

Description

Technical Field

[0001] This application relates to the technical field of cosmetics, and particularly to a composition containing alpine plant extracts and ergothioneine and its applications. Background Art

[0002] Photoaging is the skin aging change caused by long-term cumulative exposure to sunlight. Ultraviolet rays penetrate through the epidermis to reach the papillary dermis, resulting in the formation of sunburn cells in the epidermis. Ultraviolet rays can cause direct DNA damage by promoting the formation of cyclopyrimidine dimers; it can also promote the formation of other photoproducts such as reactive oxygen species, matrix metalloproteinases, and inflammatory factor IL-6 related to skin cancer and skin aging. Once the skin is exposed to ultraviolet radiation, multiple signaling pathways in skin cells that are responsible for promoting photoaging and skin cancer by regulating cell growth, proliferation, and survival are activated, leading to changes in the skin. In dermal cells such as fibroblasts, ultraviolet rays will inhibit the synthesis of type I procollagen. At the same time, the overexpression of matrix metalloproteinases leads to a decrease in the content of collagen and elastin, degrading the structural proteins that maintain skin elasticity in the dermis.

[0003] According to the principle of physical sunscreen, using raw materials such as titanium dioxide and zinc oxide to reflect sunlight to achieve the sunscreen effect. The quality of such products depends on the treatment quality of the reflective particles. Generally, ultraviolet rays can still penetrate through the gaps between the particles to reach the skin. However, this method has the following disadvantages: the product contains a large amount of reflective particles, which are easy to clog pores, generally relatively thick, not suitable for general sensitive skin and acne-prone skin, inconvenient to use as it requires makeup removal after use, generally only used when going out, and can only resist the damage of part of the ultraviolet rays, and can only passively resist light pollution and photoaging.

[0004] Combined with chemical ultraviolet absorbers, it resists light pollution by reflecting and absorbing harmful ultraviolet rays. Since the chemical sunscreen agent molecules will be absorbed by the skin, the process of absorbing ultraviolet rays occurs inside the skin and is cleared by human metabolism. However, this method has the following disadvantages: chemical sunscreen agents have certain irritation to the skin, the product has a high oil content, which is easy to cause acne formation, not suitable for general sensitive skin and acne-prone skin, inconvenient to use as it requires makeup removal after use, generally only used when going out, with relatively strong limitations, and can only passively resist light pollution and photoaging.

[0005] Using blue light absorbers such as calendula extract, sophora japonica extract, and hesperidin to prevent blue light in sunlight and LED blue light from reaching the skin and causing damage. However, this method has the following disadvantages: only applicable to the protection of LED blue light, cannot resist the light damage caused by sunlight, and can only passively resist the damage of LED blue light, and cannot actively resist the photoaging caused by LED blue light.

[0006] Therefore, it is still necessary to develop a composition that can resist light pollution, soothe and inhibit inflammation from the inside of the skin, and actively resist photoaging. Summary of the Invention

[0007] To solve at least one of the above technical problems, the present application provides a composition containing alpine plant extracts and ergothioneine, including: 0.005 - 0.8 parts by weight of saffron extract, 0.01 - 0.03 parts by weight of Scutellaria amoena extract, 0.1 - 0.4 parts by weight of Leontopodium alpinum extract, 0.0005 - 0.005 parts by weight of Rhododendron lapponicum extract, and 0.001 - 0.15 parts by weight of ergothioneine.

[0008] Through the above technical solutions, the present application takes the growth environment of numerous plant components as the screening idea. High altitude - pure and less polluted, high sunlight - strong anti - ultraviolet ability. Alpine plants are preferably used as the main force of the composition for anti - photoaging and antioxidant. The selected alpine plant extracts complement each other. Components such as saffron extract, Scutellaria amoena flower / leaf / stem extract, Leontopodium alpinum extract, Rhododendron lapponicum extract, and ergothioneine cooperate with each other through different action pathways, which can significantly improve the skin care effect. Saffron extract is rich in antioxidant components (crocin and crocetin), which can effectively neutralize free radicals. Crocin and crocetin have strong antioxidant properties, can inhibit lipid peroxidation, protect cell membranes and collagen from oxidative damage, and at the same time promote skin microcirculation and improve the skin's self - repair ability. Scutellaria amoena extract has anti - inflammatory and soothing effects. By inhibiting the release of inflammatory mediators, it reduces skin inflammatory reactions and enhances the skin barrier function. Leontopodium alpinum extract is famous for its strong moisturizing and anti - aging properties. It is rich in flavonoids, phenolic acids, polysaccharides and terpenoids. By neutralizing free radicals (such as reactive oxygen species ROS) generated after ultraviolet (UV) irradiation, it reduces the damage of oxidative stress to skin cells. It can also promote DNA repair by inhibiting matrix metalloproteinases (MMPs) in the skin and reduce the damage of ultraviolet rays to the cell genetic material. Rhododendron lapponicum extract further enhances the antioxidant and anti - inflammatory effects, and at the same time promotes collagen synthesis and improves skin elasticity. Ergothioneine, as an efficient antioxidant, can protect cells from the damage of ultraviolet rays and other environmental factors and delay skin aging. These components cooperate with each other through multiple action pathways such as antioxidant, anti - inflammatory, moisturizing, and promoting collagen synthesis. They can not only effectively resist the damage of the external environment to the skin, but also deeply nourish and repair the skin, thus achieving a better skin care effect and making the skin healthier, younger and more radiant. Under this system, saffron extract and Leontopodium alpinum extract show significant synergistic effects in improving skin anti - photoaging, soothing and antioxidant aspects.

[0009] Preferably, the composition containing alpine plant extracts and ergothioneine includes: 0.4 - 0.8 parts by weight of saffron extract, 0.01 - 0.03 parts by weight of Scutellaria amoena C. H. Wright extract, 0.1 - 0.4 parts by weight of Leontopodium alpinum Cass. extract, 0.0005 - 0.005 parts by weight of Rhododendron lapponicum (L.) Wahl. extract, and 0.01 - 0.1 parts by weight of ergothioneine.

[0010] Preferably, the composition containing alpine plant extracts and ergothioneine further includes a penetration enhancer, and the penetration enhancer includes 0.000001 - 0.00001 parts by weight of decapeptide - 4 and 0.000001 - 0.00001 parts by weight of glycoprotein.

[0011] Through the above - mentioned technical solution, decapeptide - 4 helps the skin restore firmness and elasticity by promoting the production of collagen and elastin. Glycoprotein enhances the adhesion between cells, improving the skin's moisturizing ability and barrier function.

[0012] Preferably, the weight ratio of the saffron extract to the Leontopodium alpinum Cass. extract is (1 - 8):1.

[0013] Through the above - mentioned technical solution, when the weight ratio of the saffron extract to the Leontopodium alpinum Cass. extract is controlled within a certain range, in this system, the saffron extract and the Leontopodium alpinum Cass. extract can exert greater performance advantages, achieving better anti - photoaging, soothing, and whitening effects.

[0014] In the second aspect of the present application, the present application provides an application of the above - mentioned composition containing alpine plant extracts and ergothioneine in the preparation of anti - photoaging, soothing, and whitening cosmetics or skin care products.

[0015] In the third aspect of the present application, the present application provides a skin care product, including the above - mentioned composition containing alpine plant extracts and ergothioneine, and the skin care product is any one of liquid, lotion, cream, oily or powdery.

[0016] Preferably, the mass percentage of the composition containing alpine plant extracts and ergothioneine in the skin care product is 0.9405 - 1.0116%.

[0017] Preferably, the skin care product further comprises at least one of a chelating agent, a humectant, a thickening agent, an emulsifier, an emollient, a preservative, a solvent and a pH regulator. The chelating agent is disodium EDTA or tetrasodium EDTA. The humectant is selected from at least one of butylene glycol, glycerol, propylene glycol and vegetable oil. The thickening agent is selected from at least one of ammonium acryloyldimethyltaurate / VP copolymer, sodium polyacryloyldimethyltaurate, sodium acrylate / sodium acryloyldimethyltaurate copolymer, acrylamide / sodium acryloyldimethyltaurate copolymer, xanthan gum and carbomer. The emulsifier is selected from at least one of cetearyl glucoside, PEG-100 stearate, sucrose stearate, methyl glucoside sesquistearate and cetearyl olivate / sorbitan olivate. The emollient is selected from at least one of triglyceride caprylic / capric acid, polydimethylsiloxane, caprylic / capric triglyceride, squalane, sweet almond oil, jojoba oil, isododecane and diisopropyl dimer dilinoleate.

[0018] Preferably, the skin care product is a cream. By mass percentage, the cream comprises: 0.4-0.8% of saffron extract, 0.01-0.03% of Scutellaria amoena extract, 0.1-0.4% of Leontopodium alpinum extract, 0.0005-0.005% of Rhododendron ferrugineum extract, 0.01-0.1% of ergothioneine, 0.000002-0.00002% of penetration enhancer, 0.01-0.04% of chelating agent, 4-11% of humectant, 1-4% of thickening agent, 2-4% of emulsifier, 2-10% of emollient, 0.1-1% of preservative and the balance water.

[0019] Through the above technical solution, the composition containing alpine plant extracts and ergothioneine can be prepared into a cream after adding auxiliary materials such as a chelating agent, a humectant, a thickening agent, an emulsifier, an emollient and a preservative, which can improve the stability of the product, enhance the moisturizing effect, improve the skin feel and texture, and enhance the safety of the product.

[0020] Preferably, the skin care product is in the form of a cream. By mass percentage, the cream comprises: 0.4 - 0.8% of saffron extract, 0.01 - 0.03% of Scutellaria amoena extract, 0.1 - 0.4% of Leontopodium alpinum extract, 0.0005 - 0.005% of Rhododendron ferrugineum extract, 0.01 - 0.1% of ergothioneine, 0.000001 - 0.00001% of decapeptide - 4, 0.000001 - 0.00001% of glycoprotein, 0.01 - 0.05% of disodium EDTA, 3 - 8% of butylene glycol, 1 - 4% of glycerol, 0.1 - 0.8% of acryloyldimethyltaurate / VP copolymer, 1.2 - 1.8% of cetearyl alcohol, 1.5 - 2.5% of cetearyl alcohol or cetearyl glucoside, 1.2 - 1.8% of PEG - 100 stearate or glyceryl stearate, 3 - 7% of caprylic / capric triglyceride, 1 - 3% of polydimethylsiloxane, 3 - 7% of caprylic / capric triglyceride, 1 - 3% of polydimethylsiloxane, 0.5 - 1% of preservative, and the balance of water.

[0021] Preferably, the skin care product is in the form of a serum. By mass percentage, the serum comprises: 0.4 - 0.8% of saffron extract, 0.01 - 0.03% of Scutellaria amoena extract, 0.1 - 0.4% of Leontopodium alpinum extract, 0.0005 - 0.005% of Rhododendron ferrugineum extract, 0.01 - 0.1% of ergothioneine, 0.000002 - 0.00002% of penetration enhancer, 0.01 - 0.04% of chelating agent, 4 - 11% of humectant, 0.1 - 0.5% of thickener, 1 - 3% of pH regulator, 0.1 - 1% of preservative, and the balance of water.

[0022] Through the above - mentioned technical solution, the composition containing alpine plant extracts and ergothioneine is prepared into a serum after adding excipients such as chelating agents, humectants, thickeners, pH regulators, and preservatives. This serum has excellent stability, moisturizing properties, and can improve the feel and texture.

[0023] Preferably, by mass percentage, the serum comprises: 0.4 - 0.8% of saffron extract, 0.01 - 0.03% of Scutellaria amoena extract, 0.1 - 0.4% of Leontopodium alpinum extract, 0.0005 - 0.005% of Rhododendron ferrugineum extract, 0.01 - 0.1% of ergothioneine, 0.000001 - 0.00001% of decapeptide - 4, 0.000001 - 0.00001% of glycoprotein, 0.01 - 0.04% of disodium EDTA, 3 - 8% of butylene glycol, 1 - 3% of glycerol, 0.05 - 0.2% of xanthan gum, 0.05 - 0.3% of carbomer, 1 - 3% of sodium hydroxide, 0.1 - 1% of preservative, and the balance of water.

[0024] In summary, the present application includes at least one of the following beneficial technical effects: 1. The present application provides a composition containing alpine plant extracts and ergothioneine. The composition includes saffron extract, alpine scutellaria extract, alpine edelweiss extract, alpine rose rhododendron extract, and ergothioneine. These ingredients cooperate with each other through multiple action pathways such as antioxidant, anti-inflammatory, moisturizing, and promoting collagen synthesis. They can not only effectively resist the damage of the external environment to the skin, but also deeply nourish and repair the skin, thus achieving better anti-photoaging, soothing, and antioxidant skin care effects.

[0025] 2. The present application also provides a skin care product containing the above composition containing alpine plant extracts and ergothioneine. The skin care product has excellent anti-photoaging, soothing, and antioxidant skin care effects. Detailed implementation manners

[0026] The present invention will be further described below in conjunction with specific embodiments. However, the following embodiments are only used to illustrate the present invention and should not be regarded as limiting the scope of the present invention. For those not specified in the following embodiments, they are carried out according to conventional conditions or conditions recommended by the manufacturer. The methods used, unless otherwise specified, are all conventional methods well known in the art. The consumables and reagents used, unless otherwise specified, are all commercially available. Unless otherwise stated, the professional and scientific terms used herein have the same meaning as those familiar to those skilled in the art. In addition, any method or material similar or equivalent to the described content can also be applied to the present invention. The saffron extract is purchased from Jiangxi Yisenyuan Plant Spice Co., Ltd.; the alpine scutellaria extract, ALPAFLOR SCUTELLARIA CB, is purchased from DSM Vitamins (Shanghai) Co., Ltd., the alpine edelweiss extract is Edelweiss extract C, purchased from Guangzhou Luojie Biotechnology Co., Ltd., the alpine rose rhododendron extract, is purchased from Guangzhou Chengzhi Trading Co., Ltd., with the brand name Alpenrose flower (CH) Extract Glycerine unpreserved, ergothioneine is purchased from Jiangsu Jinsan Biotechnology Co., Ltd.; decapeptide-4 is purchased from Fujian Longsheng Biotechnology Co., Ltd.; glycoprotein is purchased from Fujian Longsheng Biotechnology Co., Ltd.; acryloyldimethyltaurine / VP copolymer, with the brand name: KLEASOL AVC, is purchased from SNF SA; disodium EDTA is purchased from: Nouryon Chemicals (Ningbo) Co., Ltd.; cetearyl alcohol, with the brand name: RFA C1618, is purchased from Global Green Chemicals Public Company Limited.

[0027] Example 1 A cream containing an alpine plant extract and ergothioneine, and its component composition is shown in Table 1 below.

[0028] Table 1 The preparation method of the above cream containing an alpine plant extract and ergothioneine includes the following steps: 1) Add deionized water to a stirring pot, heat it to 78 - 82 °C, and sequentially add a humectant, a chelating agent, a thickener, and the preservative p-hydroxyacetophenone, and homogenize for 3 - 5 minutes until uniform. 2) Mix the emollient and the emulsifier, heat them to 78 - 82 °C, stir until completely melted, then add them to the stirring pot, and homogenize for 3 - 5 minutes until emulsification is complete.

[0029] 3) When the mixture obtained by homogenization in step 2) is cooled to 40 °C, add the composition containing the alpine plant extract and ergothioneine, and the preservative 1,2 - hexanediol, and stir (10 - 30 rpm) until uniform to obtain the cream containing the alpine plant extract and ergothioneine.

[0030] Example 2 Example 2 provides a cream containing an alpine plant extract and ergothioneine. The difference between Example 2 and Example 1 is only that the mass percentages of the saffron extract and the edelweiss extract are different. In Example 2, the mass percentage of the saffron extract is 0.45%, and the mass percentage of the edelweiss extract is 0.45%.

[0031] Example 3 Example 3 provides a cream containing an alpine plant extract and ergothioneine. The difference between Example 3 and Example 1 is only that the mass percentages of the saffron extract and the edelweiss extract are different. In Example 3, the mass percentage of the saffron extract is 0.8%, and the mass percentage of the edelweiss extract is 0.1%.

[0032] Example 4 Example 4 provides a cream containing an alpine plant extract and ergothioneine. The difference between Example 4 and Example 1 is only that the raw material components are different. The raw material components in Example 4 are shown in Table 2 below.

[0033] Table 2 Example 5 Example 5 provides a cream containing an alpine plant extract and ergothioneine. The difference between Example 5 and Example 1 is only that the raw material components are different. The raw material components in Example 5 are shown in Table 3 below.

[0034] Table 3 Comparative Example 1 Comparative Example 1 provides a cream containing an alpine plant extract and ergothioneine. The difference between Comparative Example 1 and Example 1 is only that the saffron extract is replaced with an equal mass of Leontopodium alpinum extract.

[0035] Comparative Example 2 Comparative Example 2 provides a cream containing an alpine plant extract and ergothioneine. The difference between Comparative Example 2 and Example 1 is only that the Leontopodium alpinum extract is replaced with an equal mass of saffron extract.

[0036] Comparative Example 3 Comparative Example 3 provides a cream containing an alpine plant extract and ergothioneine. The difference between Comparative Example 3 and Example 1 is only that the Rhododendron lapponicum extract is not added.

[0037] Comparative Example 4 Comparative Example 4 provides a cream containing an alpine plant extract and ergothioneine. The difference between Comparative Example 4 and Example 1 is only that the Scutellaria amoena extract is not added.

[0038] Comparative Example 5 Comparative Example 5 provides a cream containing an alpine plant extract. The difference between Comparative Example 5 and Example 1 is only that ergothioneine is not added.

[0039] Detection 1) Perform a photoaging effect test on the creams containing the alpine plant extract and ergothioneine obtained in the examples and comparative examples. Establish a UV-induced photoaging model cell culture: Culture HaCaT cells in DMEM medium supplemented with 10% heat-inactivated fetal bovine serum (FBS) in an incubator at 37°C and 5% CO2.

[0040] UV radiation treatment: Select HaCaT cells in the logarithmic growth phase and treat the cells with UVB radiation (80 mJ / cm 2 ) to establish a photoaging model.

[0041] The creams of the compositions containing alpine plant extracts and ergothioneine obtained in the examples and comparative examples were added to the culture medium at 10 mg / L respectively and treated for 24 h. At the same time, a blank control group without UV radiation treatment and without adding cream and a UV group with UV radiation treatment and without adding cream were set up. The cell viability was detected, and the calculation formula for cell viability was: Cell viability = (Dexperimental group - Dblank control group) / (DUV group - Dblank control group), where Dexperimental group was the absorbance OD value of the experimental group at 450 nm, Dblank control group was the absorbance OD value of the blank control group at 450 nm, and DUV group was the absorbance OD value of the UV group at 450 nm. The results are shown in Table 4.

[0042] Table 4 Comparing Comparative Examples 1 and 2 with Example 1 and combining with Table 4, it can be seen that in this system, saffron extract and leontopodium alpinum extract show a significant synergistic effect in improving skin photoaging resistance.

[0043] Comparing Comparative Example 3 with Example 1 and combining with Table 4, it can be seen that when the rhododendron ferrugineum extract is not added, the photoaging resistance effect of the cream on the skin is significantly reduced.

[0044] Comparing Comparative Example 4 with Example 1 and combining with Table 4, it can be seen that when the scutellaria baicalensis georgi extract is not added, the photoaging resistance effect of the cream on the skin is significantly reduced.

[0045] Comparing Comparative Example 5 with Example 1 and combining with Table 4, it can be seen that when ergothioneine is not added, the photoaging resistance effect of the cream on the skin is significantly reduced.

[0046] Comparing Examples 2 and 3 with Example 1 and combining with Table 4, it can be seen that the weight ratio of saffron extract and the leontopodium alpinum extract will significantly affect the photoaging resistance effect of the cream on the skin. When the weight ratio of saffron extract and the leontopodium alpinum extract is (1 - 8):1, the cream has the best photoaging resistance effect on the skin.

[0047] Comparing Examples 4 and 5 with Example 1 and combining with Table 4, it can be seen that when the composition containing alpine plant extracts and ergothioneine includes 0.4 - 0.8 parts by weight of saffron extract, 0.01 - 0.03 parts by weight of scutellaria baicalensis georgi extract, 0.1 - 0.4 parts by weight of leontopodium alpinum extract, 0.0005 - 0.005 parts by weight of rhododendron ferrugineum extract, and 0.01 - 0.1 parts by weight of ergothioneine, the cream prepared therefrom has a good photoaging resistance effect on the skin.

[0048] 2) The soothing effect of the creams of the compositions containing alpine plant extracts and ergothioneine obtained in Examples 1-3 and Comparative Examples 1-5 was tested. RAW264.7 cells were cultured in DMEM medium containing 10% FBS in an incubator at 37°C and 5% CO2. RAW264.7 cells were stimulated with 200 ng / mL of LPS. After LPS stimulation, the levels of TNF-α and IL-6 cytokines were significantly upregulated. The creams of the compositions containing alpine plant extracts and ergothioneine obtained in Examples 1-3 and Comparative Examples 1-5 were added to the medium at 2.5 mg / L respectively and treated for 24 h. At the same time, a blank control group without LPS stimulation and without adding the cream and an LPS group with LPS stimulation and without adding the cream were set up. The levels of TNF-α and IL-6 were detected. The results are shown in Table 5.

[0049] Table 5 Comparing Comparative Examples 1 and 2 with Example 1 and combining with Table 5, it can be seen that in this system, saffron extract and leontopodium alpinum extract show a significant synergistic effect in skin soothing.

[0050] Comparing Comparative Example 3 with Example 1 and combining with Table 5, it can be seen that when the alpine rhododendron extract is not added, the soothing effect of the cream on the skin is significantly reduced.

[0051] Comparing Comparative Example 4 with Example 1 and combining with Table 5, it can be seen that when the alpine scutellaria extract is not added, the soothing effect of the cream on the skin is significantly reduced.

[0052] Comparing Comparative Example 5 with Example 1 and combining with Table 5, it can be seen that when ergothioneine is not added, the soothing effect of the cream on the skin is significantly reduced.

[0053] Comparing Examples 2 and 3 with Example 1 and combining with Table 5, it can be seen that the weight ratio of saffron extract and the leontopodium alpinum extract will significantly affect the soothing effect of the cream on the skin. When the weight ratio of saffron extract and the leontopodium alpinum extract is (1-8):1, the cream has the best soothing effect on the skin.

[0054] 3) An in vitro DPPH free radical scavenging test was carried out on the creams of the compositions containing alpine plant extracts and ergothioneine obtained in Examples 1-3 and Comparative Examples 1-5. The creams of the compositions containing alpine plant extracts and ergothioneine obtained in Examples 1-3 and Comparative Examples 1-5 were diluted with PBS to a concentration of 40 μg / mL respectively to prepare the test solutions. A DPPH ethanol solution with a concentration of 0.1 mmol / L was prepared. Table 6 shows the reaction system of the in vitro DPPH free radical scavenging test.

[0055] Table 6 Reaction system for in vitro DPPH free radical scavenging test Place the reaction system at room temperature and react in the dark for 30 min. After the reaction is completed, read the absorbance OD value at 517 nm, and calculate the scavenging rate of the sample on DPPH free radicals according to the following formula.

[0056] In the formula, C1 is the absorbance OD value of the blank DPPH system at 517 nm; C2 is the absorbance OD value of the blank DPPH-free system at 517 nm; T1 is the absorbance OD value of the sample group with DPPH system at 517 nm; T2 is the absorbance OD value of the sample group without DPPH system at 517 nm.

[0057] The test results of Examples 1-3 and Comparative Examples 1-5 are shown in Table 7.

[0058] Table 7 Example Scavenging rate of DPPH free radicals Example 1 56.9％ Example 2 65.1％ Example 3 63.9％ Comparative Example 1 22.3％ Comparative Example 2 26.2％ Comparative Example 3 38.1％ Comparative Example 4 35.9％ Comparative Example 5 17.9％ Comparing Comparative Examples 1 and 2 with Example 1 and combining with Table 7, it can be seen that in this system, saffron extract and Leontopodium alpinum extract show significant synergistic effects in terms of antioxidant.

[0059] Comparing Comparative Example 3 with Example 1 and combining with Table 7, it can be seen that when Leontopodium discolor extract is not added, the antioxidant effect of the cream on the skin is significantly reduced.

[0060] Comparing Comparative Example 4 with Example 1 and combining with Table 7, it can be seen that when Scutellaria amoena extract is not added, the antioxidant effect of the cream on the skin is significantly reduced.

[0061] Comparing Comparative Example 5 with Example 1 and combining with Table 7, it can be seen that when ergothioneine is not added, the antioxidant effect of the cream on the skin is significantly reduced.

[0062] Comparing Examples 2 and 3 with Example 1 and combining with Table 7, it can be seen that the weight ratio of saffron extract and the Leontopodium alpinum extract will significantly affect the antioxidant effect of the cream on the skin. When the weight ratio of saffron extract and the Leontopodium alpinum extract is (1-8):1, the antioxidant effect of the cream on the skin is the best.

[0063] Example 6 The example provides an essence containing a composition of alpine plant extract and ergothioneine, and its component composition is shown in Table 8 below.

[0064] Table 8 Preparation method of essence containing alpine plant extract and ergothioneine, comprising the following steps: 1) Add deionized water to a stirring pot and heat to 80 - 85 °C. Sequentially add a humectant, a chelating agent, a thickening agent, and the preservative p - hydroxyacetophenone, and stir until completely dissolved; 2) Homogenize the mixture obtained in step 1). When the temperature is lowered to 40 °C, sequentially add a pH regulator, the preservative 1,2 - hexanediol, and the composition containing alpine plant extract and ergothioneine, and stir evenly to obtain the essence containing alpine plant extract and ergothioneine.

[0065] Comparative Example 6 Comparative Example 6 provides an essence containing alpine plant extract and ergothioneine. The difference between Comparative Example 6 and Example 6 is only that the saffron extract is replaced with an equal mass of Leontopodium alpinum extract.

[0066] Comparative Example 7 Comparative Example 7 provides an essence containing alpine plant extract and ergothioneine. The difference between Comparative Example 7 and Example 6 is only that the Leontopodium alpinum extract is replaced with an equal mass of saffron extract.

[0067] Detection 4) Conduct a photo - aging effect test on the essences containing alpine plant extract and ergothioneine obtained in Example 6 and Comparative Examples 6 and 7 Establish a UV - induced photo - aging model cell culture: Culture HaCaT cells in DMEM medium supplemented with 10% heat - inactivated fetal bovine serum (FBS), and culture in an incubator at 37 °C and 5% CO2.

[0068] UV radiation treatment: Select HaCaT cells in the logarithmic growth phase and use UVB radiation (dose 80 mJ / cm 2 ) to treat the cells to establish a photo - aging model.

[0069] Respectively add the essences containing alpine plant extract and ergothioneine obtained in Example 6 and Comparative Examples 6 and 7 to the medium at 10 mg / L and treat for 24 h. At the same time, set up a blank control group without UV radiation and without adding essence and a UV group with UV radiation and without adding essence. Detect cell viability. The calculation formula for cell viability is: Cell viability=(Dexperimental group - Dblank control group) / (DUV group - Dblank control group), where Dexperimental group is the absorbance OD value of the experimental group at 450 nm, Dblank control group is the absorbance OD value of the blank control group at 450 nm, and DUV group is the absorbance OD value of the UV group at 450 nm. The results are shown in Table 9.

[0070] Table 9 Example Cell viability Example 6 151.53％ UV group 100％ Blank control group 206.52％ Comparative Example 6 117.53％ Comparative Example 7 116.96％ From the comparison between Comparative Examples 6 and 7 and Example 6 in conjunction with Table 9, it can be seen that under this system, saffron extract and Leontopodium alpinum extract show a significant synergistic effect in improving skin photoaging resistance.

[0071] The above are all preferred embodiments of this application. Without restricting the protection scope of this application based on this, therefore: All equivalent changes made according to the structure, shape, and principle of this application should be covered within the protection scope of this application.

Claims

1. A composition containing an alpine plant extract and ergothioneine, characterized in that, Comprising: 0.005 - 0.8 parts by weight of saffron extract, 0.01 - 0.03 parts by weight of Scutellaria baicalensis Georgi extract, 0.1 - 0.4 parts by weight of Leontopodium alpinum Cass. extract, 0.0005 - 0.005 parts by weight of Rhododendron ferrugineum L. extract, and 0.001 - 0.15 parts by weight of ergothioneine.

2. The composition containing alpine plant extract and ergothioneine according to claim 1, characterized in that, Comprising: 0.4 - 0.8 parts by weight of saffron extract, 0.01 - 0.03 parts by weight of Scutellaria baicalensis Georgi extract, 0.1 - 0.4 parts by weight of Leontopodium alpinum Cass. extract, 0.0005 - 0.005 parts by weight of Rhododendron ferrugineum L. extract, and 0.01 - 0.1 parts by weight of ergothioneine.

3. The composition containing alpine plant extract and ergothioneine according to claim 1, characterized in that, The composition containing alpine plant extracts and ergothioneine further comprises a penetration enhancer, which comprises 0.000001 - 0.00001 parts by weight of decapeptide - 4 and 0.000001 - 0.00001 parts by weight of glycoprotein.

4. The composition containing alpine plant extract and ergothioneine according to claim 1, characterized in that, The weight ratio of the saffron extract to the Leontopodium alpinum Cass. extract is (1 - 8):

1.

5. Use of the composition containing alpine plant extracts and ergothioneine according to any one of claims 1 - 4 in the preparation of anti - photoaging, soothing, and whitening cosmetics or skin care products.

6. A skin care product, characterized in that, Comprising the composition containing alpine plant extracts and ergothioneine according to any one of claims 1 - 4, and the skin care product is any one of liquid, emulsion, cream, oil - based or powder.

7. The skin care product according to claim 6, characterized in that, The mass percentage of the composition containing alpine plant extracts and ergothioneine in the skin care product is 0.9405 - 1.0116%.

8. The skin care product according to claim 6, characterized in that, The skin care product further comprises at least one of a chelating agent, a humectant, a thickening agent, an emulsifier, an emollient, a preservative, a solvent, and a pH regulator. The chelating agent is disodium EDTA or tetrasodium EDTA. The humectant is selected from at least one of butylene glycol, glycerol, propylene glycol, and vegetable oil. The thickening agent is selected from at least one of ammonium acryloyldimethyltaurate / VP copolymer, sodium acryloyldimethyltaurate, sodium acrylate / sodium acryloyldimethyltaurate copolymer, acrylamide / sodium acryloyldimethyltaurate copolymer, xanthan gum, and carbomer. The emulsifier is selected from at least one of cetearyl glucoside, PEG - 100 stearate, sucrose stearate, methyl glucoside sesquistearate, and cetearyl olivate / sorbitan olivate. The emollient is selected from at least one of caprylic / capric triglyceride, polydimethylsiloxane, caprylic / capric acid triglyceride, squalane, sweet almond oil, jojoba oil, isododecane, and diisopropyl dimer dilinoleate.

9. The skin care product according to claim 6, wherein The skin care product is a cream. By mass percentage, the cream comprises: 0.4 - 0.8% of saffron extract, 0.01 - 0.03% of Scutellaria amoena extract, 0.1 - 0.4% of Leontopodium alpinum extract, 0.0005 - 0.005% of Rhododendron ferrugineum extract, 0.01 - 0.1% of ergothioneine, 0.000002 - 0.00002% of penetration enhancer, 0.01 - 0.04% of chelating agent, 4 - 11% of humectant, 1 - 4% of thickener, 2 - 4% of emulsifier, 2 - 10% of emollient, 0.1 - 1% of preservative and the balance of water.

10. The skin care product according to claim 6, characterized in that, The skin care product is a serum. By mass percentage, the serum comprises: 0.4 - 0.8% of saffron extract, 0.01 - 0.03% of Scutellaria amoena extract, 0.1 - 0.4% of Leontopodium alpinum extract, 0.0005 - 0.005% of Rhododendron ferrugineum extract, 0.01 - 0.1% of ergothioneine, 0.000002 - 0.00002% of penetration enhancer, 0.01 - 0.04% of chelating agent, 4 - 11% of humectant, 0.1 - 0.5% of thickener, 1 - 3% of pH regulator, 0.1 - 1% of preservative and the balance of water.

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