Specific carbon source denitrification domestication activated sludge culture device and application thereof

Abstract

The invention relates to the technical field of sewage treatment, and discloses a specific carbon source denitrification domestication activated sludge culture device and application thereof.The specific carbon source denitrification domestication activated sludge culture device comprises a sludge culture reactor, a carbon source water inlet bucket, a nitrogen source water inlet bucket, a sludge stirring device, a timer, a water inlet pump and a water outlet pump; the carbon source water inlet pipe and the nitrogen source water inlet pipe converge through a three-way pipe joint and are pumped into the sludge culture reactor through a water inlet pump, a water outlet of the reactor is connected with a water outlet pipe and sludge is discharged through the water outlet pump, and a carbon source, a nitrogen source, a phosphorus source and various trace elements are regularly added into the sludge culture reactor. Wherein the adding amount of the phosphorus source and the trace elements and the carbon-nitrogen ratio of the inlet water are kept in a preset proportion. Activated sludge is domesticated by using different carbon sources, and a phosphorus source and trace elements are added to support microbial growth. And determining the denitrification rate, analyzing a metagenome community structure, a metabolic pathway and enzyme activity, and exploring the metabolism similarity of the carbon source. The composite denitrification effect of similar carbon sources is verified, and the development of a novel efficient composite carbon source is guided.

Description

Technical Field

[0001] The present invention relates to the technical field of sewage treatment, and specifically to a device for culturing activated sludge by denitrification with a specific carbon source and its application. Background Art

[0002] In the field of sewage treatment, with the increasingly strict TN emission standards in the "Draft for Soliciting Opinions on the Pollutant Discharge Standards for Municipal Sewage Treatment Plants" and the low carbon-nitrogen ratio of urban domestic sewage in China resulting in insufficient carbon sources for biological denitrification and nitrogen removal, sewage treatment plants often add external carbon sources. However, existing carbon sources have prominent problems in terms of type selection, operation regulation, etc. For example, methanol is toxic and has high risks in transportation and storage, sodium acetate has high costs and high sludge production rates, sugar substances have slow denitrification rates and are prone to sludge bulking, etc., and operation regulation is not precise, resulting in exceeded effluent standards, increased costs, increased carbon emissions, and more problems in sludge treatment and disposal. Under the goal of "carbon peak and carbon neutrality", the sewage treatment industry urgently needs a low-carbon and efficient way of using carbon sources to solve the problems of carbon source selection and efficient utilization, achieve quality improvement and efficiency increase, and low-carbon operation. The research and development of a device for culturing activated sludge by denitrification with a specific carbon source is imminent.

[0003] Previous devices have obvious shortcomings in treating high-concentration nitrate nitrogen wastewater. In terms of carbon source control, it is impossible to precisely control the type of carbon source, the dosing amount, and operating conditions, and it is difficult to simulate the denitrification process under different carbon source environments, so it is impossible to deeply explore the denitrification metabolic characteristics of carbon sources by combining the measurement and analysis of denitrification rates, activated sludge community structures, etc. This makes it impossible for previous devices to effectively improve the biological activity of activated sludge in the system, and the wastewater treatment effect is poor. At the same time, due to the lack of a scientific experimental platform and technical support, it is difficult for previous devices to develop efficient composite carbon sources. The development can only rely on experience and trial and error, with low efficiency and poor effects. The device for culturing activated sludge by denitrification with a specific carbon source can make up for these deficiencies. Summary of the Invention

[0004] In view of the deficiencies of the prior art, the present invention provides a device for culturing activated sludge by denitrification with a specific carbon source and its application, which solves the problems that previous devices cannot effectively improve the biological activity of activated sludge in the system and are difficult to develop efficient composite carbon sources that can quickly achieve treatment effects when treating high-concentration nitrate nitrogen wastewater.

[0005] To achieve the above objectives, the present invention is realized through the following technical solutions: A device for culturing activated sludge by denitrification with a specific carbon source includes a sludge culture reactor, a carbon source inlet bucket, a nitrogen source inlet bucket, a sludge stirring device, a timer, a feed pump, and a discharge pump. The carbon source inlet pipe and the nitrogen source inlet pipe converge through a three-way pipe joint and are pumped into the sludge culture reactor via the feed pump. The outlet of the reactor is connected to the outlet pipe, and the sludge is discharged via the discharge pump.

[0006] By adopting the above technical solutions: Through denitrification domestication of activated sludge with different carbon sources, a phosphorus source and various trace elements are added to the influent water to ensure the growth requirements of microorganisms. The denitrification rate changes of the sludge domesticated with each carbon source for various carbon sources are measured, and the metagenome is combined to analyze the activated sludge community structure and metabolic pathways, as well as the measurement of key enzyme activities to explore the denitrification metabolism similarities of various carbon sources, and then verify the denitrification effect after the combination of carbon sources with similar metabolism, so as to guide the development of new and efficient composite carbon sources.

[0007] Preferably, in the sludge culture reactor, a carbon source, a nitrogen source, a phosphorus source and various trace elements are regularly added, and the addition amounts of the phosphorus source and the trace elements are kept in a preset ratio with the influent carbon-nitrogen ratio.

[0008] Preferably, the carbon source and the nitrogen source are added through independent influent systems, and the phosphorus source and the trace elements are pre-configured into a concentrated solution and added in an intermittent manner.

[0009] Preferably, the phosphorus source and various trace elements are configured into a concentrated solution according to the required amounts in a certain ratio, sealed and stored in a brown volumetric flask, and added to the influent bucket when configuring the influent carbon source and nitrogen source.

[0010] Preferably, an application method of domesticated activated sludge for the biological doubling process is used for the specific carbon source denitrification domesticated activated sludge culture device described above, and the domesticated activated sludge is used to treat high-nitrate nitrogen wastewater in a sewage treatment plant.

[0011] Preferably, an application method of domesticated activated sludge for the biological doubling process includes the following steps: S1. After concentrating and filtering the sludge mixture to remove impurities, inoculate it into the sludge culture reactor; S2. Determine the device operation cycle and drainage ratio. Each cycle includes influent, stirring, sedimentation and effluent, and the program is automatically controlled by a timer; S3. Adopt the gradient concentration method for carbon source denitrification domestication, and synchronously add the phosphorus source and trace elements to maintain the growth of microorganisms; S4. After the domestication starts, monitor the effluent COD and nitrate nitrogen concentration, and measure the changes in the extended COD and nitrate nitrogen concentration during the denitrification stage after a certain period. Determine the domestication situation of the sludge by measuring the denitrification rate, and determine the domestication cycle and response time of various carbon sources to the activated sludge; S5. After the domestication of the activated sludge is completed, carry out flocculation precipitation to obtain high-concentration activated sludge.

[0012] Preferably, for an application method of domesticated activated sludge for the biological doubling process, the activated sludge domesticated by using the specific carbon source denitrification domesticated activated sludge culture device is added to the aeration tank system for treating high-concentration nitrate nitrogen wastewater by the activated sludge method.

[0013] Preferably, a method for developing an efficient composite denitrifying carbon source is used for the specific carbon source denitrification acclimated activated sludge culture device, and the method includes the following steps: S1. First, measure the change in the denitrification rate of the sludge acclimated with each carbon source for various carbon sources. S2. Combine metagenomic analysis to analyze the activated sludge community structure and metabolic pathways. S3. Explore the denitrification metabolic similarity of various carbon sources by measuring the key enzyme activity. S4. Verify the denitrification effect after compounding carbon sources with similar metabolism to guide the development of a new type of efficient composite carbon source.

[0014] Preferably, a system for treating high-concentration nitrate nitrogen wastewater by the activated sludge method is used for the specific carbon source denitrification acclimated activated sludge culture device, and further includes an aeration tank and a sedimentation tank, and the sludge culture reactor is connected to the aeration tank.

[0015] Preferably, a process for treating high-concentration nitrate nitrogen wastewater by the activated sludge method is used for the system for treating high-concentration nitrate nitrogen wastewater by the activated sludge method, and the process includes the following steps: S1. Sewage flows into the aeration tank, and the high-concentration activated sludge obtained by the specific carbon source denitrification acclimated activated sludge culture device is supplemented into the aeration tank for aeration and mixing. S2. The mixed liquid after the purification of the activated sludge enters the sedimentation tank, and the suspended activated sludge and other solid substances in the mixed liquid precipitate and are separated from the water, and the clarified sewage is discharged from the system as treated water. S3. The sludge concentrated after sedimentation is discharged from the bottom of the sedimentation tank, and most of it is returned to the aeration tank as inoculation sludge to ensure the suspended solid concentration and microbial concentration in the aeration tank. S4. The remaining sludge is discharged from the system.

[0016] Working principle: First, accurately add carbon and nitrogen sources. Through the design of separate dosing of the carbon source inlet bucket and the nitrogen source inlet bucket, the flow direction of the solution is controlled by independent pipelines and inlet valves. The carbon source inlet bucket provides a specific carbon source (such as sodium acetate, methanol, etc.) as the only carbon source, and the nitrogen source inlet bucket adds nitrogen sources such as nitrates and supporting phosphorus sources and trace elements; this separate dosing method effectively avoids the pre-reaction of carbon and nitrogen and ensures the precise control of the culture environment; Then, perform periodic acclimation culture. Set a standardized operation cycle (inlet water - stirring - sedimentation - outlet water) through a timer, and strictly control each link: in the inlet water stage, quantitatively add carbon and nitrogen sources; in the stirring stage, create an anoxic environment to promote denitrification; in the sedimentation stage, achieve the separation of mud and water; in the outlet water stage, discharge the supernatant. This periodic operation simulates the actual sewage treatment conditions and promotes the directional acclimation of microorganisms. Finally, the sludge performance is optimized. Under precisely controlled environmental conditions, the device promotes the formation of tightly structured zoogloea by microorganisms through continuous selective pressure. The stirring device maintains a uniform mixing state to avoid sludge sedimentation; sludge is regularly discharged to control the sludge age, and dominant bacterial communities with good settling performance (SVI < 80 mL / g) and strong adsorption capacity are screened out. After multi-cycle cultivation, the sludge concentration can be increased to over 5000 mg / L, fully meeting the sludge supplementation requirements of the biological treatment system.

[0017] The present invention provides a device for cultivating specific carbon source denitrification-acclimated activated sludge and its application, having the following beneficial effects: 1. In the present invention, the activated sludge is denitrification-acclimated with different carbon sources, and a phosphorus source and various trace elements are added to the influent water to ensure the growth requirements of microorganisms. The denitrification rate changes of the sludge acclimated with each carbon source for various carbon sources are measured, and the metagenome is combined to analyze the activated sludge community structure and metabolic pathways, as well as the determination of key enzyme activities to explore the denitrification metabolism similarities of various carbon sources, and then verify the denitrification effect after the combination of carbon sources with similar metabolism, guiding the development of new and efficient composite carbon sources.

[0018] 2. In the present invention, under the combined action of key components such as a sludge cultivation reactor, a carbon source inlet bucket, a nitrogen source inlet bucket, a sludge stirring device, an inlet pump, an outlet pump, and a timer, the water inlet, stirring, sedimentation, and water outlet are automatically controlled in each cycle by determining the operation cycle, drainage ratio, and sludge age. The system operates stably, promoting the formation of high-quality zoogloea by microorganisms, and the rapid proliferation of activated sludge, meeting the sludge supplementation requirements of the nitrite nitrogen wastewater biological treatment system.

[0019] 3. In the present invention, due to the unique advantages of the device for cultivating specific carbon source denitrification-acclimated activated sludge, denitrification-acclimation work can be carried out for a variety of different carbon sources. A phosphorus source and various trace elements are scientifically and reasonably added in the water inlet link to create a good nutrient environment for the growth of microorganisms. By accurately measuring the denitrification rate changes of the acclimated sludge and combining metagenome analysis technology to deeply analyze its internal mechanism, it can provide a strong basis for the development of new and efficient composite carbon sources, and then significantly improve the biological treatment efficiency of activated sludge. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 is a perspective view of a device for cultivating specific carbon source denitrification-acclimated activated sludge of the present invention; Figure 2 is a flow chart of an application method for acclimating activated sludge for a biological doubling process of the present invention; Figure 3 is a flow chart of a development method for a high-efficiency composite denitrification carbon source of the present invention; Figure 4Process flow chart of a process for treating high-concentration nitrate nitrogen wastewater by the activated sludge method of the present invention; Figure 5 Schematic diagram of the changes in COD, NO3-N, and NO2-N concentrations after the denitrification domestication of different carbon sources of the present invention becomes stable; Figure 6 Table of sludge concentration and sedimentation performance domesticated with different carbon sources of the present invention; Figure 7 Schematic diagram of the dilution curve of 16S sequencing of sludge domesticated with different carbon sources of the present invention; Figure 8 Schematic diagram of the shannon index and chao1 index of microorganisms in sludge domesticated with different carbon sources of the present invention; Figure 9 Schematic diagram of the species analysis of sludge domesticated with different carbon sources of the present invention at the phylum and genus levels; Figure 10 Thermal schematic diagram of the metabolic pathway of sludge domesticated with different carbon sources of the present invention. Detailed implementation manners

[0021] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the specification. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without making creative efforts shall fall within the protection scope of the present invention.

[0022] Please refer to the attached Figure 1 , an activated sludge culture device for denitrification domestication with a specific carbon source, comprising a sludge culture reactor, a carbon source inlet bucket, a nitrogen source inlet bucket, a sludge stirring device, a timer, a feed pump, and a discharge pump. The carbon source inlet pipe and the nitrogen source inlet pipe converge through a tee joint and are pumped into the sludge culture reactor via the feed pump, and the outlet of the reactor is connected to the outlet pipe to discharge the sludge via the discharge pump.

[0023] Specifically, an influent valve is provided in the sludge cultivation reactor to control the solution circulation and circulation direction among the sludge cultivation reactor, the carbon source inlet bucket, and the nitrogen source inlet bucket. Among them, the sludge cultivation reactor is used to complete the domestication and cultivation of activated sludge with a specific carbon source as the sole carbon source. The carbon source inlet bucket and the nitrogen source inlet bucket are used to add a certain concentration of specific carbon source, nitrogen source, phosphorus source, and various trace elements to the sludge cultivation reactor. Among them, the system operates stably, enabling microorganisms to form zoogloea with a compact structure, good adsorption effect, and excellent sedimentation performance. The activated sludge can proliferate rapidly, and the sludge concentration increases significantly, which can meet the sludge supplement demand of the nitrate nitrogen wastewater biological treatment system to the greatest extent. Among them, the activated sludge reactor is cylindrical, and inlet and outlet valves are distributed on the cylinder body. Among them, the inlet pipe and the outlet pipe are made of silica gel and are replaced regularly to prevent water leakage. The water pipe at the connection of the inlet pump and the outlet pump is made of wear-resistant silica gel pipe and is replaced regularly to prevent water leakage. When sludge domestication is carried out under cold conditions, a heat preservation strip can be wound around the sludge cultivation reactor to maintain the sludge cultivation temperature in the reactor. The outlet valve of the activated sludge reactor should not be too low to prevent the precipitation sludge from being discharged. At the same time, sludge should be discharged regularly according to the setting of the sludge age.

[0024] Please refer to the appendix Figure 1 , in the sludge cultivation reactor, carbon source, nitrogen source, phosphorus source, and various trace elements are added regularly. Among them, the dosing amounts of the phosphorus source and the trace elements are maintained at a preset ratio to the influent carbon-nitrogen ratio. The carbon source and the nitrogen source are added through independent influent systems. The phosphorus source and the trace elements are pre-configured into a concentrated solution and are added in an intermittent dosing manner. The phosphorus source and various trace elements are configured into a concentrated solution according to the required amounts in a certain ratio, sealed and stored in a brown volumetric flask, and added to the inlet bucket when configuring the influent carbon source and nitrogen source.

[0025] Specifically, first determine the device operation cycle, drainage ratio, and sludge age. Each cycle includes influent, stirring, sedimentation, and effluent, and the program is automatically controlled by a timer: ① Influent: During the influent period, the carbon source and the nitrogen source are added to the reactor separately through peristaltic pumps. The purpose of separating the influent of the carbon source and the nitrogen source is to prevent denitrification from occurring in the influent container, resulting in low influent carbon and nitrogen concentrations.

[0026] ② Stirring: In this stage, denitrification occurs in the reactor, and the denitrification reaction should proceed fully to ensure the sedimentation of the sludge.

[0027] ③ Sedimentation: In this stage, the mud and water are separated under static conditions.

[0028] ④ Effluent: The supernatant after sedimentation is discharged Please refer to the appendix Figure 2, An application method of domesticated activated sludge for the biological doubling process, which is used for the above-mentioned specific carbon source denitrification domesticated activated sludge cultivation device, and the domesticated activated sludge is used to treat high-nitrate nitrogen wastewater in a sewage treatment plant; the method includes the following steps: S1. After concentrating and filtering the sludge mixture to remove impurities, inoculate it into the sludge cultivation reactor; S2. Determine the device operation cycle and drainage ratio. Each cycle includes water inlet, stirring, sedimentation and water outlet, and the program is automatically controlled by a timer; S3. Adopt the gradient concentration method for carbon source denitrification domestication, and synchronously add phosphorus source and trace elements to maintain the growth of microorganisms; S4. After the domestication starts, monitor the effluent COD and nitrate nitrogen concentration, and measure the changes in the extended COD and nitrate nitrogen concentration during the denitrification stage after a certain period. Judge the domestication situation of the sludge by measuring the denitrification rate, and determine the domestication cycle and response time of various carbon sources to the activated sludge; S5. After the domestication of the activated sludge is completed, carry out flocculation precipitation to obtain high-concentration activated sludge.

[0029] Specifically, S1 is used to establish a high-activity initial sludge system, which can avoid the interference of impurities on the growth of microorganisms, increase the initial sludge concentration, and accelerate the domestication process; S2 is used to simulate the actual sewage treatment conditions and screen efficient denitrifying bacteria groups; S3 is used to gradually improve the carbon source adaptability and maintain the microbial metabolic balance, which can avoid the disintegration of the sludge caused by carbon source shock, and the trace elements activate the key enzymes of denitrification (such as nitrate reductase); S4 is used to quantify the domestication effect and optimize the carbon source selection; among them, the effluent COD is used to reflect the carbon source utilization efficiency (target removal rate > 90%); the nitrate nitrogen concentration is used to evaluate the denitrification effect (target removal rate > 85%); the denitrification rate is the amount of NO3 - -N degradation amount (g NO3 - -N / g VSS·h).

[0030] Please refer to the appendix Figure 2 , An application method of domesticated activated sludge for the biological doubling process, the activated sludge obtained by domesticating with a specific carbon source denitrification domesticated activated sludge cultivation device is added to the aeration tank system for treating high-concentration nitrate nitrogen wastewater by the activated sludge method.

[0031] Specifically, the application includes culturing activated sludge in the specific carbon source denitrification acclimation activated sludge culture device, using a specific carbon source as the sole carbon source to acclimate and culture the activated sludge, obtaining highly concentrated activated sludge with biological multiplication for treating high-nitrate wastewater and developing a highly efficient new denitrification carbon source; first, placing the activated sludge to be acclimated in a sludge culture reactor; performing denitrification acclimation on the activated sludge with various carbon sources respectively, adding a phosphorus source and various trace elements to ensure the growth requirements of microorganisms; after the acclimation of the activated sludge is completed, performing flocculation precipitation to obtain highly concentrated activated sludge.

[0032] Please refer to Appendix Figure 3 , a method for developing a highly efficient composite denitrification carbon source, for the above-mentioned specific carbon source denitrification acclimation activated sludge culture device, the method includes the following steps: S1. First, measure the change in the denitrification rate of the sludge acclimated with each carbon source for various carbon sources; S2. Combine metagenomic analysis to analyze the activated sludge community structure and metabolic pathways; S3. Explore the denitrification metabolic similarity of various carbon sources by measuring the activity of key enzymes; S4. Verify the denitrification effect after compounding carbon sources with similar metabolism, and guide the development of a new type of highly efficient composite carbon source.

[0033] Specifically, the function of S1 is to quantify the denitrification efficiency of different carbon sources and screen highly efficient basic carbon sources; the function of S2 is to analyze the influence of carbon sources on the microbial community and functional genes; the function of S3 is to verify the carbon source metabolic similarity and guide the compounding ratio; the function of S4 is to develop a highly efficient composite carbon source with strong synergism and low cost.

[0034] Please refer to Appendix 1, a system for treating high-concentration nitrate wastewater by the activated sludge method, for the above-mentioned specific carbon source denitrification acclimation activated sludge culture device, further including an aeration tank and a sedimentation tank, and the sludge culture reactor is connected to the aeration tank.

[0035] Specifically, it is composed of a specific carbon source denitrification acclimation activated sludge culture device, an aeration tank and a sedimentation tank, and each component works together to achieve efficient nitrogen removal. Among them, the function of the aeration tank is to complete the degradation of organic matter, ammonia nitrogen nitrification (NH4 + →NO3 - ), and denitrification (NO3 - →N2); the function of the sedimentation tank is to achieve the separation of mud and water, ensure clear effluent, and at the same time recover the activated sludge.

[0036] Please refer to Appendix Figure 4 , a process for treating high-concentration nitrate wastewater by the activated sludge method, for the above-mentioned system for treating high-concentration nitrate wastewater by the activated sludge method, the process includes the following steps: S1. Sewage flows into the aeration tank, and the high-concentration activated sludge obtained through the specific carbon source denitrification-acclimated activated sludge cultivation device is supplemented into the aeration tank, and aeration and mixing are carried out. S2. The mixed liquor after the purification of the activated sludge enters the sedimentation tank, and the suspended activated sludge and other solid substances in the mixed liquor precipitate and are separated from the water, and the clarified sewage is discharged from the system as treated water. S3. The sludge concentrated after sedimentation is discharged from the bottom of the sedimentation tank, and most of it is returned to the aeration tank as inoculation sludge to ensure the suspended solid concentration and microorganism concentration in the aeration tank. S4. The remaining sludge is discharged from the system.

[0037] Specifically, in the above high-concentration nitrate nitrogen wastewater, the concentration of nitrate nitrogen pollutants ≥ 50 mg / L; the above application includes supplementing the high-concentration activated sludge obtained through the specific carbon source denitrification-acclimated activated sludge cultivation device into the aeration tank of the system for treating high-concentration nitrate nitrogen wastewater by the activated sludge method.

[0038] Example 1: Six carbon sources (methanol, ethanol, glycerol, glucose, sucrose, starch) were used to carry out denitrification acclimation on the sludge respectively. By measuring the nitrate nitrogen concentration in the effluent every day, it was found that the response times of different carbon sources to the denitrification function of the sludge were different.

[0039] As shown in the appendix Figure 5 When the sludge denitrification acclimation was stable, the concentration changes of extended COD, NO3-N and NO2-N in each device were measured.

[0040] As shown in the appendix Figure 6 When the sludge denitrification acclimation was stable, the sludge concentration and sedimentation performance in each device were measured.

[0041] As shown in the appendix Figure 7 As shown: The microbial composition of the sludge after being acclimated with different carbon sources has large differences. Compared with the unacclimated sludge, the community diversity of the acclimated sludge generally decreases. This is because a large number of aerobic bacteria are eliminated under anoxic conditions, resulting in a decrease in the overall species diversity.

[0042] As shown in the appendix Figure 8 As shown: The glucose-acclimated sludge and glycerol-acclimated sludge have a relatively fast denitrification rate for various carbon sources. At the genus level, different carbon sources respectively enrich specific functional flora. In the similarity analysis, the sludge communities acclimated with glucose, ethanol and glycerol have a relatively high similarity, and the sludge communities acclimated with starch and sucrose have a relatively high similarity.

[0043] As shown in the appendix Figure 9As shown: Under different carbon source conditions, there are significant differences in the composition of the microbial community in the sludge. Under certain carbon source conditions, the species diversity of the microbial community is relatively high, while under other conditions it is relatively low. This indicates that the type of carbon source has an important impact on the species composition of the microbial community. Among them, Proteobacteria and Bacteroidetes are the main microbial phyla, occupying a relatively high relative abundance. At the genus level, genera such as Pseudomonas and Bacillus show different relative abundances under different carbon source conditions.

[0044] As shown in the appendix Figure 10 As shown: In the analysis of the metabolic pathways of each sample, the characteristic metabolic pathways of the sludge domesticated with different carbon sources were screened, and the similarities and differences in the metabolism of various carbon sources can be obtained in these pathways.

[0045] Although the embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A culturing device for denitrifying and domesticating activated sludge with a specific carbon source, comprising a sludge culturing reactor, a carbon source inlet bucket, a nitrogen source inlet bucket, a sludge stirring device, a timer, a water inlet pump and a water outlet pump, characterized in that, The carbon source inlet pipe and the nitrogen source inlet pipe converge through a tee joint and are pumped into the sludge cultivation reactor by an inlet water pump. The outlet of the reactor is connected to an outlet pipe, and the sludge is discharged through an outlet water pump.

2. A specific carbon source denitrification acclimation activated sludge cultivation device according to claim 1, characterized in that, In the sludge cultivation reactor, a carbon source, a nitrogen source, a phosphorus source, and various trace elements are regularly added. The addition amounts of the phosphorus source and the trace elements maintain a preset ratio with the influent carbon-nitrogen ratio.

3. A specific carbon source denitrification acclimation activated sludge cultivation device according to claim 2, characterized in that, The carbon source and the nitrogen source are added through independent inlet systems. The phosphorus source and the trace elements are pre-prepared into a concentrated solution and added in an intermittent manner.

4. A specific carbon source denitrification acclimation activated sludge cultivation device according to claim 3, characterized in that, The phosphorus source and various trace elements are configured into a concentrated solution according to the required amounts in a certain ratio and stored in a brown volumetric flask in a sealed manner, and are added to the inlet water bucket when configuring the influent carbon source and nitrogen source.

5. An application method of domesticated activated sludge for a biological doubling process, characterized in that, For a specific carbon source denitrification-acclimated activated sludge cultivation device according to any one of claims 1-4, the acclimated activated sludge is used to treat high-nitrate nitrogen wastewater in a sewage treatment plant.

6. The application method of domesticated activated sludge for a biological doubling process according to claim 5, characterized in that, The method includes the following steps: S1. After concentrating and filtering the sludge mixture to remove impurities, inoculate it into the sludge cultivation reactor; S2. Determine the device operation cycle and drainage ratio. Each cycle includes influent, stirring, sedimentation, and effluent, and the program is automatically controlled by a timer; S3. Use the gradient concentration method for carbon source denitrification acclimation, and synchronously add the phosphorus source and trace elements to maintain the growth of microorganisms; S4. After the acclimation starts, monitor the effluent COD and nitrate nitrogen concentration, and measure the changes in the extended COD and nitrate nitrogen concentration during the denitrification stage after a certain period. Judge the acclimation situation of the sludge by measuring the denitrification rate, and determine the acclimation cycle and response time of various carbon sources to the activated sludge; S5. After the activated sludge acclimation is completed, perform flocculation precipitation to obtain high-concentration activated sludge.

7. The application method of domesticated activated sludge for a biological multiplication process according to claim 6, characterized in that, The activated sludge obtained by acclimation using the specific carbon source denitrification-acclimated activated sludge cultivation device is added to the aeration tank system for treating high-concentration nitrate nitrogen wastewater by the activated sludge method.

8. A development method of an efficient composite denitrifying carbon source, characterized in that For a specific carbon source denitrification-acclimated activated sludge cultivation device according to any one of claims 1-4, the method includes the following steps: S1. First, measure the changes in the denitrification rate of the sludge acclimated with each carbon source for various carbon sources; S2. Combine metagenomic analysis to analyze the activated sludge community structure and metabolic pathways; S3. Explore the denitrification metabolism similarity of various carbon sources by measuring the key enzyme activity; S4. Verify the denitrification effect after compounding carbon sources with similar metabolism, and guide the development of new and efficient compound carbon sources.

9. A system for treating high-concentration nitrate nitrogen wastewater by the activated sludge process, characterized in that, For a specific carbon source denitrification-acclimated activated sludge cultivation device according to any one of claims 1-4, it further includes an aeration tank and a sedimentation tank, and the sludge cultivation reactor is connected to the aeration tank.

10. A process for treating high-concentration nitrate nitrogen wastewater by the activated sludge method, characterized in that, For a system for treating high-concentration nitrate nitrogen wastewater by the activated sludge method according to claim 9, the process includes the following steps: S1. The sewage flows into the aeration tank, and the high-concentration activated sludge obtained by the specific carbon source denitrification-acclimated activated sludge cultivation device is supplemented to the aeration tank for aeration and mixing; S2. The mixed liquid after the purification of the activated sludge enters the sedimentation tank. The suspended activated sludge and other solid substances in the mixed liquid precipitate and are separated from the water, and the clarified sewage is discharged from the system as treated water. S3. The sludge after sedimentation and thickening is discharged from the bottom of the sedimentation tank, and most of it is refluxed to the aeration tank as inoculation sludge to ensure the suspended solid concentration and microorganism concentration in the aeration tank; S4. The remaining sludge is discharged from the system.

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