A mucus preventing agent for freshwater lobster eggs and its use

By using an anti-sticking solution for Australian freshwater crayfish eggs and a specialized device, the problem of fertilized eggs adhering to female crayfish has been solved, improving hatching rates and production efficiency, simplifying management processes, and reducing the mortality rate of broodstock crayfish.

CN120323375BActive Publication Date: 2026-07-24重庆市水产科学研究所(重庆农垦农产品质量安全检验检测站)
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
重庆市水产科学研究所(重庆农垦农产品质量安全检验检测站)
Filing Date
2025-04-25
Publication Date
2026-07-24

AI Technical Summary

Technical Problem

During the breeding process of Australian freshwater crayfish, fertilized eggs are easily damaged due to cannibalism and territoriality, resulting in a low hatching rate. Furthermore, existing technologies make it difficult to effectively separate fertilized eggs from female crayfish, increasing management difficulty and facility requirements.

Method used

The product uses an anti-sticking solution for Australian freshwater crayfish eggs, containing albumin, vegetable oleic acid, and soy lecithin. This solution is used to soak fertilized female crayfish to prevent the fertilized eggs from adhering to the female crayfish's appendages. The fertilized eggs are then collected and incubated using a specific device, which combines water flow and gravity to achieve automatic detachment and collection.

Benefits of technology

It improved the hatching rate of fertilized eggs, reduced human intervention, lowered the mortality rate of broodstock shrimp, simplified the management process, and improved the efficiency of seedling production.

✦ Generated by Eureka AI based on patent content.

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Abstract

The application discloses a kind of Australian freshwater lobster egg anti-mucus and application thereof, it is related to lobster breeding field, including albumin 0.5%~1.5%, soybean lecithin 5%~15% and plant oil acid 5%~15%, and the rest is water.The application provides a kind of shrimp egg anti-mucus, can be soaked by the way, the female shrimp's pedipalpus cannot be adhered with the discharged spermatozoon, so that the spermatozoon of female shrimp is separated from female shrimp without mechanical or artificial strong intervention, by collecting spermatozoon and centralized hatching, the hatching rate of spermatozoon can be effectively avoided in the centralized feeding of Australian freshwater lobster, because the situation of spermatozoon membrane damage due to territoriality, so as to improve the hatching rate of spermatozoon.The application also provides a breeding device, under the cooperation of water flow, gravity and anti-mucus, can realize the spermatozoon and female shrimp separation, automatic centralized collection of separated spermatozoon, save manpower, and can shorten the time of parent shrimp in temporary pond, unified centralized hatching also reduces the workload of hatching process.
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Description

Technical Field

[0001] This invention relates to the field of lobster farming, specifically to an anti-sticking liquid for Australian freshwater lobster eggs and its application. Background Technology

[0002] Australian freshwater crayfish have a unique reproductive system, exhibiting the habit of carrying their young on their abdomens. Their egg-bearing capacity is relatively small, generally ranging from 200 to 1000 eggs. However, due to cannibalism and natural mortality, a single parent crayfish typically only produces a few dozen, at most a couple hundred, offspring, thus limiting large-scale seed production. Furthermore, Australian freshwater crayfish are highly territorial; fighting often results in broken limbs, and the eggs die along with the parent crayfish. Therefore, during the breeding process, it is necessary to reduce the density of parent crayfish, and after hatching, the parent crayfish must be removed promptly. In addition, the inconsistent hatching time of the eggs makes the unified removal of parent crayfish difficult, placing higher demands on facilities and management. Summary of the Invention

[0003] To address the aforementioned shortcomings of existing technologies, this invention provides an anti-sticking liquid for Australian freshwater crayfish eggs that can reduce egg loss during intensive aquaculture and its application.

[0004] To achieve the above-mentioned objectives, the technical solution adopted by this invention is as follows:

[0005] An anti-sticking liquid for Australian freshwater crayfish eggs is provided, comprising 0.5%–1.5% albumin, 5%–15% soybean lecithin, and 5%–15% oleic acid, with the remainder being water.

[0006] An anti-sticking liquid for Australian freshwater crayfish eggs comprises 0.5%–1.5% albumin, 5%–15% soybean lecithin, and 5%–15% vegetable oleic acid, with the remainder being water.

[0007] Furthermore, it contains 1% albumin, 9% soy lecithin, and 9% vegetable oleic acid, with the remainder being water.

[0008] The present invention also provides an application of the above-mentioned anti-sticking liquid for Australian freshwater lobster eggs, which is used to adhere to the appendages of fertilized female Australian freshwater lobsters to prevent the fertilized eggs excreted by the female lobster from adhering to the appendages of the female lobster, and to facilitate the detachment of the fertilized eggs from the female lobster.

[0009] The present invention also provides a method for culturing Australian freshwater crayfish using the above-mentioned anti-sticking liquid, comprising the following steps:

[0010] S1: Place the fertilized female shrimp in a culture pond containing anti-mucus and raise them normally;

[0011] S2: Collect the fertilized eggs that have detached from the female shrimp and fallen to the bottom of the breeding pond, and then artificially incubate the collected fertilized eggs separately.

[0012] The present invention also provides a breeding device for Australian freshwater crayfish, including a breeding pond filled with the above-mentioned anti-sticking liquid for crayfish eggs, a filter screen with a pore size larger than that of fertilized eggs but smaller than that of crayfish installed in the breeding pond, a fertilized egg collection port installed at the bottom of the breeding pond, and a fertilized egg incubation pond connected to the fertilized egg collection port.

[0013] Furthermore, it also includes a water collection component for real-time collection of fertilized eggs. The water collection component includes a water pump, the output end of which is connected to the aquaculture pond through an anti-slip solution inlet, and the input end of which is connected to a shrimp egg anti-slip solution preparation tank. The anti-slip solution inlet is located above the filter screen, and the top of the fertilized egg hatching tank is located below the filter screen.

[0014] Furthermore, the bottom of the aquaculture pond is funnel-shaped, and there are multiple anti-slippery liquid inlets, which are evenly distributed around the circumference of the aquaculture pond.

[0015] The beneficial effects of this invention are as follows:

[0016] This invention provides a shrimp egg anti-adhesion liquid that, through soaking, prevents the female shrimp's appendages from adhering to the released fertilized eggs. This allows the fertilized eggs to detach from the female shrimp without mechanical or strong human intervention. By collecting the fertilized eggs and hatching them in a centralized manner, it effectively avoids the damage to the egg membrane caused by territorial behavior when Australian freshwater crayfish are fed in groups, thereby improving the hatching rate of the fertilized eggs.

[0017] The present invention also provides an aquaculture device that, with the help of water flow, gravity and anti-slip liquid, can realize the separation of fertilized eggs from female shrimp and automatically collect the separated fertilized eggs, saving manpower and shortening the time that parent shrimp spend in the temporary holding pond. The unified centralized hatching also reduces the workload of the hatching process. Attached Figure Description

[0018] Figure 1 A schematic diagram of a farming apparatus for Australian freshwater crayfish;

[0019] Figure 2 This is a top view of the aquaculture pond.

[0020] The components include: 1. Aquaculture pond; 2. Filter screen; 3. Fertilized egg collection port; 4. Fertilized egg hatching pond; 5. Water pump; 6. Anti-slip solution inlet; and 7. Shrimp egg anti-slip solution preparation pond. Detailed Implementation

[0021] The specific embodiments of the present invention are described below to enable those skilled in the art to understand the present invention. However, it should be understood that the present invention is not limited to the scope of the specific embodiments. For those skilled in the art, various changes are obvious as long as they are within the spirit and scope of the present invention as defined and determined by the appended claims. All inventions utilizing the concept of the present invention are protected.

[0022] Example 1

[0023] Food-grade albumin (98%) from Claylan (Shaanxi) Biotechnology Co., Ltd., food-grade soybean lecithin (99%) from Anhui Weimao Biotechnology Co., Ltd., and food-grade oleic acid (99%) from Wuhan Kemike Biomedical Technology Co., Ltd. were used to prepare 10L of anti-adhesion solutions for shrimp eggs with different ratios. Tap water was used for preparation. Female shrimp carrying eggs were soaked in each anti-adhesion solution for 5–10 minutes. The total number of fertilized eggs and the number of detached eggs were counted. The anti-adhesion rate was calculated as: number of detached fertilized eggs / (number of detached fertilized eggs + number of fertilized eggs adhering to the appendages). Each ratio was tested three times. The total number of detached fertilized eggs and the total number of fertilized eggs from the three experiments were used to calculate the detachment rate. The ratios and statistical results are shown in Table 1 below.

[0024] Table 1

[0025] Group 1 0.1% 3% 3% vegetable oleic acid 50.2% Group 2 0.5% 5% 5% vegetable oleic acid 84.3% Group 3 1% 9% 9% vegetable oleic acid 90.5% Group 4 1.5% 15% 15% vegetable oleic acid 92.3% Group 5 2% 20% 20% vegetable oleic acid 74.2%* Group 6 1.5% 15% Animal oleic acid 15% 86.2%

[0026] As shown in Group 1 of Table 1, when the amounts of albumin, soy lecithin, and oleic acid in the anti-mucus solution are less than those specified in this invention, the fertilized eggs of the female shrimp cannot detach smoothly. As shown in Groups 2 to 4, the anti-mucus solution of this invention can effectively detach the fertilized eggs from the appendages of the female shrimp.

[0027] As shown in Groups 4 and 6, although the desorption rate using animal oleic acid can reach 86.2%, it is still inferior to that using vegetable oleic acid.

[0028] Among them, the anti-mucus solution in group 5 caused the female shrimp to struggle after being placed in the solution, making the operation inconvenient and causing significant stimulation to the parent shrimp. Although the detachment rate was high, the cost was also high. The significant stimulation to the parent shrimp also had a significant impact on the subsequent release and hatching of fertilized eggs.

[0029] Example 2

[0030] like Figure 1-2 As shown, an Australian freshwater crayfish farming device includes a farming pond 1, which is filled with the aforementioned anti-sticking liquid for crayfish eggs. A filter screen 2 with a pore size larger than that of fertilized eggs but smaller than that of crayfish is installed in the farming pond 1. A fertilized egg collection port 3 is provided at the bottom of the farming pond 1, and the fertilized egg collection port 3 is connected to a fertilized egg hatching pond 4.

[0031] It also includes a water collection component for real-time collection of fertilized eggs. The water collection component includes a water pump 5. The output end of the water pump 5 is connected to the breeding pond 1 through an anti-slip solution inlet 6. The input end of the water pump 5 is connected to a shrimp egg anti-slip solution preparation pond 7. The anti-slip solution inlet 6 is located above the filter screen 2. The top of the fertilized egg hatching pond 4 is located below the filter screen 2.

[0032] The bottom of the aquaculture pond 1 is funnel-shaped, and there are multiple anti-slippery liquid inlets 6, which are evenly distributed around the aquaculture pond 1.

[0033] After Australian freshwater crayfish are fertilized, the female crayfish are placed in a rearing tank 1 containing an anti-fouling solution for their eggs. Tank 1 serves as a temporary holding tank. The fertilized female crayfish releases fertilized eggs in tank 1. Meanwhile, the anti-fouling solution is continuously prepared in a tank 7 and pumped evenly into tank 1 via a water pump 5. Under the gentle impact of the water flow, gravity, and the anti-fouling solution, the fertilized eggs released by the female crayfish fall towards the fertilized egg collection port 3 at the bottom of tank 1. The filter screen 2 prevents the female crayfish from passing through it. The fertilized eggs are unaffected because the funnel-shaped bottom of the rearing pond 1 allows the fertilized eggs to naturally gather together with the water flow. They then flow through the fertilized egg collection port 3 to the fertilized egg hatching pond 4. The entire process requires no manual or other mechanical intervention. The fertilized eggs will not be damaged by the gentle water flow. When there are too many fertilized eggs and the fertilized egg collection port 3 becomes clogged, the water pump 5 stops working, pausing the introduction of shrimp egg anti-sticking fluid into the rearing pond 1. The fertilized eggs are allowed to flow to the fertilized egg hatching pond 4 under gravity. The water pump 5 is restarted after the clog is cleared. The fertilized egg hatching pond 4 creates a suitable hatching environment for the collected fertilized eggs to hatch.

Claims

1. An anti-sticking liquid for Australian freshwater crayfish eggs, characterized in that, It contains 0.5%~1.5% albumin, 5%~15% soy lecithin, and 5%~15% oleic acid, with the remainder being water; The anti-sticking liquid is used to adhere to the appendages of the female Australian freshwater lobster after fertilization, preventing the fertilized eggs released by the female from adhering to the appendages and facilitating the detachment of the fertilized eggs from the female.

2. The anti-sticking liquid for Australian freshwater crayfish eggs according to claim 1, characterized in that, It contains 0.5%~1.5% albumin, 5%~15% soy lecithin and 5%~15% vegetable oleic acid, with the remainder being water.

3. The anti-sticking liquid for Australian freshwater crayfish eggs according to claim 2, characterized in that, It contains 1% albumin, 9% soy lecithin, and 9% vegetable oleic acid, with the remainder being water.

4. A method for culturing Australian freshwater crayfish using the anti-slip solution described in claim 1 or 2, characterized in that, Includes the following steps: S1: Place the fertilized female shrimp in a culture pond containing anti-mucus and raise them normally; S2: Collect the fertilized eggs that have detached from the female shrimp and fallen to the bottom of the breeding pond, and then artificially incubate the collected fertilized eggs separately.

5. A farming apparatus for Australian freshwater crayfish, characterized in that, The system includes a breeding pond (1), which is filled with the anti-sticking liquid for shrimp eggs as described in claim 1. The breeding pond (1) is equipped with a filter screen (2) with a pore size larger than that of fertilized eggs and smaller than that of lobsters. The bottom of the breeding pond (1) is equipped with a fertilized egg collection port (3), which is connected to a fertilized egg hatching pond (4).

6. The aquaculture apparatus according to claim 5, characterized in that, It also includes a water flow collection component for real-time collection of fertilized eggs, the water flow collection component including a water pump (5), the output end of the water pump (5) is connected to the breeding pond (1) through an anti-slip liquid inlet (6), and the input end of the water pump (5) is connected to a shrimp egg anti-slip liquid preparation pond (7); the anti-slip liquid inlet (6) is located above the filter screen (2); the top of the fertilized egg hatching pond (4) is located below the filter screen (2).

7. The aquaculture apparatus according to claim 6, characterized in that, The bottom of the aquaculture pond (1) is funnel-shaped, and there are multiple anti-mucus inlets (6), which are evenly distributed around the aquaculture pond (1).