Anti-wrinkle composition containing acetyl hexapeptide-8 as well as preparation method and application of anti-wrinkle composition

Abstract

The invention discloses an anti-wrinkle composition containing acetyl hexapeptide-8 as well as a preparation method and application of the anti-wrinkle composition. The anti-wrinkle composition comprises polypeptide lipidosome, a stevia rebaudiana extract, a stevia rebaudiana leaf / stem extract and a paullinia cupana seed extract, the polypeptide lipidosome comprises a lipid membrane and polypeptide located in the lipid membrane, and the polypeptide comprises acetyl hexapeptide-8, copper peptide and nonapeptide-1. The acetyl hexapeptide-8 is used for reducing the release of acetylcholine so as to effectively relax muscles, and a good instant anti-wrinkle effect is achieved; and copper peptide and nonapeptide-1 are supplemented to promote the expression of collagen and the like, stimulate the regeneration of a corium layer structure and bring a good long-acting anti-wrinkle effect. Meanwhile, the polypeptide combination is delivered by adopting lipidosome, so that the transdermal performance and the long-acting anti-wrinkle effect of the polypeptide combination are enhanced. According to the anti-wrinkle composition, the stevia rebaudiana leaf / stem extract serves as an instant anti-wrinkle reinforcing component, the stevia rebaudiana leaf / stem extract is matched with the copper peptide and the nonapeptide-1, a better collagen stimulation effect is achieved, the long-acting anti-wrinkle effect of the composition is enhanced, and the paullinia cupana cacao seed extract can strengthen skin barriers and improve the skin compactness.

Description

Technical Field

[0001] This application relates to the technical field of polypeptides, and in particular to an anti-wrinkle composition containing acetyl hexapeptide-8, its preparation method and application. Background Art

[0002] Acetyl hexapeptide-8, also known as Argireline, is a high-quality anti-wrinkle polypeptide ingredient. Its mechanism of action is to inhibit neurotransmitter release and block muscle contraction. The anti-wrinkle effect of acetyl hexapeptide-8 is significant and the side effects are small, so it has become a research hotspot in the anti-aging field and is widely used in various anti-aging personal care products. However, simply inhibiting muscle contraction cannot completely improve the skin because the skin includes multiple parts such as the epidermis, dermis, and muscle. Therefore, researchers have designed a comprehensive anti-aging plan that takes into account the epidermis, dermis, and muscle layers to comprehensively improve problems such as epidermal structure relaxation, dermal collagen degradation, and free radical damage, so as to achieve full cortex repair.

[0003] On the other hand, from the perspective of instant anti-wrinkle, acetyl hexapeptide-8 is often compounded with Tagetes erecta extract. However, the action target of acetyl hexapeptide is acetylcholine, and the action target of Tagetes erecta extract is the sodium ion channel. The sensitivity difference between the two is significant, and the actual application effect is difficult to guarantee. Moreover, the addition of other auxiliary components in the composition may also cause a decrease in the activity of the active ingredients, and the anti-wrinkle effect needs to be further tested. Therefore, it is necessary to be able to propose a composition with a higher anti-wrinkle effect. Summary of the Invention

[0004] This application aims to solve at least one of the technical problems existing in the prior art. For this purpose, this application proposes an anti-wrinkle composition containing acetyl hexapeptide-8, its preparation method and application.

[0005] In the first aspect of this application, an anti-wrinkle composition is provided. The anti-wrinkle composition includes polypeptide liposomes, Tagetes erecta extract, Stevia rebaudiana leaf / stem extract, and Theobroma grandiflorum seed extract. The polypeptide liposomes include a lipid membrane and a polypeptide located inside the lipid membrane. The polypeptide includes acetyl hexapeptide-8, copper peptide, and peptide-1.

[0006] The anti-wrinkle composition according to the embodiments of this application has at least the following beneficial effects:

[0007] Using acetyl hexapeptide-8 to reduce acetylcholine release, effectively relax muscles, relieve dynamic wrinkles, and have good anti-wrinkle effects; supplemented with copper peptides and nonapeptide-1 to promote the expression of collagen, elastin, and laminin, stimulate the regeneration of the dermal layer structure, strengthen the epidermis-dermis connection, improve sagging and relaxation, enhance firmness, and bring good long-term anti-wrinkle effects. At the same time, the polypeptide combination is delivered by liposomes to avoid the obstruction of the stratum corneum and enzymatic decomposition, enhance its transdermal performance and long-term anti-wrinkle effect. In addition, the composition also adds a variety of plant extracts. The extract of Centaurea cyanus L. acts as an instant anti-wrinkle multi-angle reinforcing component by inhibiting the activity of sodium ion channels, while the extract of Stevia rebaudiana Bertoni leaves / stems is combined with copper peptides and nonapeptide-1, having a better effect of stimulating collagen production and reducing the wrinkle area, further enhancing its long-term anti-wrinkle effect. Finally, the extract of Theobroma grandiflorum can strengthen the skin barrier and improve skin firmness, thereby forming a more efficient anti-wrinkle effect synergistically from multiple aspects and angles.

[0008] In some embodiments of the present application, the mass ratio of acetyl hexapeptide-8, copper peptide, and nonapeptide-1 is 1:(0.1 - 0.5):(1 - 2). Among them, the mass ratio of acetyl hexapeptide-8 to copper peptide can be 1:0.1, 1:0.2, 1:0.3, 1:0.4, 1:0.5; the mass ratio of acetyl hexapeptide-8 to nonapeptide-1 can be 1:1, 1:1.1, 1:1.2, 1:1.3, 1:1.4, 1:1.5, 1:1.6, 1:1.7, 1:1.8, 1:1.9, 1:2.

[0009] In some embodiments of the present application, the raw materials of the lipid membrane include phospholipids, ceramides, and polyethylene glycol. For the above combination of acetyl hexapeptide-8, copper peptide, and nonapeptide-1, the lipid membrane of the liposome uses phospholipids as the main film-forming component, adds ceramides to improve the penetration efficiency of the liposome, and is combined with polyethylene glycol modification to enhance the sustained release effect of the long-term anti-wrinkle components of copper peptide and nonapeptide-1, so as to achieve the synergistic improvement of instant anti-wrinkle and long-term anti-wrinkle effects.

[0010] In some embodiments of the present application, in the raw materials of the lipid membrane, the mass ratio of phospholipids, ceramides, and polyethylene glycol is 1:(0.01 - 0.1):(0.01 - 0.1). Among them, the mass ratio of phospholipids to ceramides can be 1:0.01, 1:0.02, 1:0.03, 1:0.04, 1:0.05, 1:0.06, 1:0.07, 1:0.08, 1:0.09, 1:0.1, and the mass ratio of phospholipids to polyethylene glycol can be 1:0.01, 1:0.02, 1:0.03, 1:0.04, 1:0.05, 1:0.06, 1:0.07, 1:0.08, 1:0.09, 1:0.1.

[0011] In some embodiments of the present application, the types of phospholipids include at least one of soy lecithin, hydrogenated soy lecithin, lysophosphatidylcholine, hydrogenated lysophosphatidylcholine, phosphatidylcholine, hydrogenated phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, and phosphatidylserine.

[0012] In some embodiments of the present application, the mass ratio of the Tagetes erecta extract, the Stevia rebaudiana leaf / stem extract, and the Theobroma grandiflorum seed extract is 1:(0.5 - 2):(0.01 - 0.1). Among them, the mass ratio of the Tagetes erecta extract to the Stevia rebaudiana leaf / stem extract can be 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:1.1, 1:1.2, 1:1.3, 1:1.4, 1:1.5, 1:1.6, 1:1.7, 1:1.8, 1:1.9, 1:2, and the mass ratio of the Tagetes erecta extract to the Theobroma grandiflorum seed extract can be 1:0.01, 1:0.02, 1:0.03, 1:0.04, 1:0.05, 1:0.06, 1:0.07, 1:0.08, 1:0.09, 1:0.1.

[0013] In some embodiments of the present application, the mass ratio of the polypeptide to the Tagetes erecta extract is 1:(0.01 - 100). Among them, the mass ratio of the polypeptide to the Tagetes erecta extract is 1:0.01, 1:0.02, 1:0.03, 1:0.04, 1:0.05, 1:0.06, 1:0.07, 1:0.08, 1:0.09, 1:0.1, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8, 1:0.9, 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10, 1:20, 1:30, 1:40, 1:50, 1:60, 1:70, 1:80, 1:90, 1:100.

[0014] The second aspect of the present application provides a method for preparing an anti-wrinkle composition, and the preparation method includes the following steps:

[0015] Prepare polypeptide liposomes;

[0016] Mix the polypeptide liposomes, the Tagetes erecta extract, the Stevia rebaudiana leaf / stem extract, and the Theobroma grandiflorum seed extract to obtain the anti-wrinkle composition.

[0017] In some embodiments of the present application, the method for preparing polypeptide liposomes includes at least one of the thin film hydration method, the reverse evaporation method, the injection-ultrasound combination method, etc.

[0018] In some embodiments of the present application, the method for preparing polypeptide liposomes includes the following steps:

[0019] Mix the raw materials of the lipid membrane to obtain an oil-phase mixture;

[0020] Mix acetyl hexapeptide-8, copper peptide and nonapeptide-1 with an aqueous solvent to obtain an aqueous-phase mixture;

[0021] Mix the oil-phase mixture with the aqueous-phase mixture, homogenize and emulsify to obtain polypeptide liposomes.

[0022] In some embodiments of the present application, the mixing of the oil-phase mixture is carried out at 60-80 °C, such as 60 °C, 62 °C, 64 °C, 65 °C, 66 °C, 68 °C, 70 °C, 72 °C, 74 °C, 75 °C, 76 °C, 78 °C, 80 °C.

[0023] In some embodiments of the present application, the mixing of the aqueous-phase mixture is carried out at 60-80 °C, such as 60 °C, 62 °C, 64 °C, 65 °C, 66 °C, 68 °C, 70 °C, 72 °C, 74 °C, 75 °C, 76 °C, 78 °C, 80 °C.

[0024] In some embodiments of the present application, the temperature of homogenization is 60-80 °C, such as 60 °C, 62 °C, 64 °C, 65 °C, 66 °C, 68 °C, 70 °C, 72 °C, 74 °C, 75 °C, 76 °C, 78 °C, 80 °C.

[0025] In some embodiments of the present application, emulsification includes primary emulsification and secondary emulsification, and the secondary emulsification is high-pressure emulsification at 500-1500 bar.

[0026] In some embodiments of the present application, the temperature of primary emulsification is 40-60 °C, such as 40 °C, 42 °C, 44 °C, 45 °C, 46 °C, 48 °C, 50 °C, 52 °C, 54 °C, 55 °C, 56 °C, 58 °C, 50 °C.

[0027] In some embodiments of the present application, the temperature of secondary emulsification is 20-40 °C, such as 20 °C, 22 °C, 24 °C, 25 °C, 26 °C, 28 °C, 30 °C, 32 °C, 34 °C, 35 °C, 36 °C, 38 °C, 40 °C.

[0028] In the third aspect of the present application, a cosmetic is provided, and the cosmetic includes the aforementioned anti-wrinkle composition.

[0029] In some embodiments of the present application, the dosage form of the cosmetic includes any one of aqueous solutions, ointments, creams, emulsions, gels, hydrogels, aerosols, sprays, injections.

[0030] In some embodiments of the present application, it further includes at least one of an emulsifier and a thickener.

[0031] In some embodiments of the present application, the emulsifier includes polyglycerol esters, and the thickener includes at least one of xanthan gum, carbomer, and cellulose.

[0032] In some embodiments of the present application, the polyglycerol ester is a polyglycerol ester formed by polyglycerol and at least one of C6-C30 fatty acids, for example, it can be C6, C7, C8, C9, C10, C11, C12, C13, C14, C15, C16, C17, C18, C19, C20, C21, C22, C23, C24, C25, C26, C27, C28, C29, C30. In some of these embodiments, the fatty acid is a saturated fatty acid or an unsaturated fatty acid. In some of these embodiments, the degree of polymerization of glycerol in the polyglycerol ester is 2-10, for example, it can be 2, 3, 4, 5, 6, 7, 8, 9, 10. In some of these embodiments, the C6-C30 fatty acids include at least one of stearic acid, isostearic acid, oleic acid, lauric acid, palmitic acid, myristic acid, etc.

[0033] In some embodiments of the present application, the polyglycerol ester includes at least one of polyglyceryl-10 myristate, polyglyceryl-10 stearate, polyglyceryl-10 oleate, polyglyceryl-10 palmitate, polyglyceryl-2 oleate, and polyglyceryl-2 triisostearate.

[0034] In some embodiments of the present application, the mass percentage of the emulsifier in the cosmetic is 0.1-5%, for example, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.8%, 1%, 2%, 3%, 4%, 5%.

[0035] In some embodiments of the present application, the mass percentage of the thickener in the cosmetic is 0.1-5%, for example, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.8%, 1%, 2%, 3%, 4%, 5%.

[0036] In some embodiments of the present application, the cosmetic includes at least one of essence, cream, eye cream, facial mask, and hydrodermabrasion needle.

[0037] The embodiments of the present application also provide the use of the aforementioned anti-wrinkle composition or the aforementioned cosmetic in removing wrinkles.

[0038] The embodiments of the present application also provide the use of the aforementioned anti-wrinkle composition or the aforementioned cosmetic in the preparation of a product for removing wrinkles.

[0039] The embodiments of the present application also provide a method for removing wrinkles, including using the aforementioned anti-wrinkle composition or the aforementioned cosmetic.

[0040] Additional aspects and advantages of the present application will be given in part in the following description, become apparent in part from the following description, or be learned through the practice of the present application. Detailed Embodiments

[0041] The concept of the present application and the resulting technical effects will be clearly and completely described below in conjunction with embodiments to fully understand the purpose, features, and effects of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all embodiments. Other embodiments obtained by those skilled in the art based on the embodiments of the present application without creative efforts shall fall within the scope of protection of the present application.

[0042] Embodiments of the present application will be described in detail below. The described embodiments are exemplary and are only used to explain the present application, and should not be construed as a limitation of the present application.

[0043] In the description of the present application, the meaning of "several" is more than one, the meaning of "multiple" is more than two, "greater than", "less than", "exceeding", etc. are understood as not including the number itself, and "above", "below", "within", etc. are understood as including the number itself. If there is a description of "first" and "second", it is only for the purpose of distinguishing technical features and should not be understood as indicating or implying relative importance or implicitly indicating the quantity of the indicated technical features or implicitly indicating the sequence relationship of the indicated technical features.

[0044] Unless otherwise defined, all technical and scientific terms used in the present application have the same meaning as commonly understood by those skilled in the technical field to which the present application belongs. The terms used herein are only for the purpose of describing the embodiments of the present application and are not intended to limit the present application. The specific features, structures, materials, or characteristics described may be combined in a suitable manner in any one or more embodiments or examples.

[0045] The present application will be described below in conjunction with specific embodiments.

[0046] Embodiment 1

[0047] This embodiment provides an anti-wrinkle composition, which includes polypeptide liposome, Gomphrena globosa extract, Stevia rebaudiana leaf / stem extract, Theobroma grandiflorum seed extract, as well as p-hydroxyacetophenone, 1,2-hexanediol, xanthan gum, sodium carboxymethylcellulose, and deionized water. The preparation process of the anti-wrinkle composition is as follows:

[0048] 1. Preparation of polypeptide liposome

[0049] 1a. Take 5 parts by mass of hydrogenated soy lecithin, 0.5 parts by mass of ceramide, and 0.5 parts by mass of polyethylene glycol-400 and put them into an oil phase pot and heat to 70 °C to obtain an oil phase mixture.

[0050] 1b. Take 1 part by mass of acetyl hexapeptide-8, 0.5 part by mass of copper peptide, 1.5 parts by mass of nonapeptide-1, 50 parts by mass of deionized water, and 14 parts by mass of glycerol, and put them into a vacuum emulsifying pot and heat to 60 °C to obtain an aqueous phase mixture.

[0051] 1c. Put the oil phase mixture from step 1a into a vacuum emulsifying pot, maintain at 60 °C, and use a homogenizer to homogenize at a speed of 4000 rpm for 3 minutes to complete one emulsification, and cool to 50 °C to obtain a primary emulsified product.

[0052] 1d. Put the primary emulsified product into a high-pressure emulsifying machine, and perform secondary emulsification at 40 °C and 1000 bar to obtain polypeptide liposomes.

[0053] 2. Preparation of anti-wrinkle composition

[0054] 2a. Take 0.8 part by mass of p-hydroxyacetophenone, 0.4 part by mass of 1,2-hexanediol, 0.2 part by mass of xanthan gum, 0.1 part by mass of sodium carboxymethyl cellulose, and 60 parts by mass of deionized water, mix them, heat to 85 °C, and stir until completely dissolved to obtain a composition matrix.

[0055] 2b. After the composition matrix is cooled to 45 °C, add 10 parts by mass of polypeptide liposomes (containing 5 wt% polypeptide), 2 parts by mass of Centaurea cyanus extract, 1 part by mass of Stevia rebaudiana leaf / stem extract, 0.2 part by mass of Theobroma grandiflorum seed extract, and 25.3 parts by mass of deionized water, mix well, and cool to 25 °C to obtain the anti-wrinkle composition.

[0056] Example 2

[0057] This example provides an anti-wrinkle composition. The difference from Example 1 is that in step 1b, the amount of copper peptide becomes 0.1 part by mass, and the amount of deionized water is adjusted to keep the total mass unchanged.

[0058] Example 3

[0059] This example provides an anti-wrinkle composition. The difference from Example 1 is that in step 1b, the amount of nonapeptide-1 becomes 1 part by mass, and the amount of deionized water is adjusted to keep the total mass unchanged.

[0060] Example 4

[0061] This example provides an anti-wrinkle composition. The difference from Example 1 is that in step 1b, the amount of nonapeptide-1 becomes 2 parts by mass, and the amount of deionized water is adjusted to keep the total mass unchanged.

[0062] Example 5

[0063] This example provides an anti-wrinkle composition, which is different from Example 1 in that in step 1a, hydrogenated soy lecithin is replaced with phosphatidylcholine of equal mass.

[0064] Example 6

[0065] This example provides an anti-wrinkle composition, which is different from Example 1 in that in step 1a, hydrogenated soy lecithin is replaced with phosphatidylethanolamine of equal mass.

[0066] Example 7

[0067] This example provides an anti-wrinkle composition, which is different from Example 1 in that in step 1a, the amount of ceramide becomes 0.05 parts by mass.

[0068] Example 8

[0069] This example provides an anti-wrinkle composition, which is different from Example 1 in that in step 1a, the amount of polyethylene glycol becomes 0.05 parts by mass.

[0070] Example 9

[0071] This example provides an anti-wrinkle composition, which is different from Example 1 in that in step 2b, the amount of stevia leaf / stem extract becomes 4 parts by mass, and the amount of deionized water is adjusted to keep the total mass unchanged.

[0072] Example 10

[0073] This example provides an anti-wrinkle composition, which is different from Example 1 in that in step 2b, the amount of Theobroma grandiflorum seed extract becomes 0.02 parts by mass, and the amount of deionized water is adjusted to keep the total mass unchanged.

[0074] Example 11

[0075] This example provides an anti-wrinkle composition, which is different from Example 1 in that in step 2b, the amount of polypeptide liposome becomes 1 part by mass, and the amount of deionized water is adjusted to keep the total mass unchanged.

[0076] Example 12

[0077] This example provides an anti-wrinkle composition, which is different from Example 1 in that in step 2b, the amount of extract becomes 0.5 parts by mass of Centaurea cyanus extract, 0.3 parts by mass of stevia leaf / stem extract, 0.05 parts by mass of Theobroma grandiflorum seed extract, and the amount of deionized water is adjusted to keep the total mass unchanged.

[0078] Comparative Example 1

[0079] This comparative example provides an anti-wrinkle composition, which is different from Example 1 in that the polypeptide in the polypeptide liposome is only acetyl hexapeptide-8 with equal total mass.

[0080] Comparative Example 2

[0081] This comparative example provides an anti-wrinkle composition, which is different from Example 1 in that the polypeptide in the polypeptide liposome is only the copper peptide with the total mass unchanged.

[0082] Comparative Example 3

[0083] This comparative example provides an anti-wrinkle composition, which is different from Example 1 in that the polypeptide in the polypeptide liposome is only the nonapeptide-1 with the total mass unchanged.

[0084] Comparative Example 4

[0085] This comparative example provides an anti-wrinkle composition, which is different from Example 1 in that the total mass of the polypeptide in the polypeptide liposome remains unchanged, and the mass ratio of acetyl hexapeptide-8, copper peptide and nonapeptide-1 is 1:2:1.5.

[0086] Comparative Example 5

[0087] This comparative example provides an anti-wrinkle composition, which is different from Example 1 in that the total mass of the polypeptide in the polypeptide liposome remains unchanged, and the mass ratio of acetyl hexapeptide-8, copper peptide and nonapeptide-1 is 1:0.5:0.2.

[0088] Comparative Example 6

[0089] This comparative example provides an anti-wrinkle composition, which is different from Example 1 in that there is only the extract of Centaurea cyanus under the condition that the total mass of the extract remains unchanged.

[0090] Comparative Example 7

[0091] This comparative example provides an anti-wrinkle composition, which is different from Example 1 in that there is only the extract of Stevia rebaudiana Bertoni leaves / stems under the condition that the total mass of the extract remains unchanged.

[0092] Comparative Example 8

[0093] This comparative example provides an anti-wrinkle composition, which is different from Example 1 in that there is only the extract of Theobroma grandiflorum seeds under the condition that the total mass of the extract remains unchanged.

[0094] Comparative Example 9

[0095] This comparative example provides an anti-wrinkle composition, which is different from Example 1 in that under the condition that the total mass of the extract remains unchanged, the mass ratio of the extract of Centaurea cyanus, the extract of Stevia rebaudiana Bertoni leaves / stems and the extract of Theobroma grandiflorum seeds is 1:5:0.1.

[0096] Comparative Example 10

[0097] This comparative example provides an anti-wrinkle composition, which is different from Example 1 in that, when the total mass of the extracts remains unchanged, the mass ratio of the Centaurea cyanus extract, the Stevia rebaudiana leaf / stem extract, and the Theobroma grandiflorum seed extract is 1:0.5:3.

[0098] Comparative Example 11

[0099] This comparative example provides an anti-wrinkle composition, which is different from Example 1 in that, when the total mass of the extracts remains unchanged, the mass ratio of the Centaurea cyanus extract, the Stevia rebaudiana leaf / stem extract, and the Theobroma grandiflorum seed extract is 1:0.1:0.1.

[0100] Safety test

[0101] The skin patch test was carried out in accordance with the requirements of the "Technical Specifications for Cosmetics Safety" (2015 Edition).

[0102] Test subjects: Aged 18 - 60 years old, with an equal number of men and women. For each of Examples 1 - 12 and Comparative Examples 1 - 11, 3 people were randomly selected. Those who had used antihistamines in the past week or immunosuppressants in the past month, those who had applied any anti-inflammatory drugs to the test site in the past two months, those with inflammatory skin diseases that were not clinically cured, insulin-dependent diabetes patients, asthma or other chronic respiratory disease patients who were receiving treatment, those who had received anti-cancer chemotherapy in the past 6 months, immunodeficiency or autoimmune disease patients, lactating or pregnant women, those who had undergone bilateral mastectomy and bilateral axillary lymph node resection, those whose test sites were affected by scars, pigmentation, atrophy, nevus flammeus or other defects and thus affected the determination of test results, those who participated in other clinical trials, those with highly sensitive constitutions, those who were not willing to participate or could not complete the specified content according to the test requirements were excluded.

[0103] Test method: Select a qualified patch test device with an area not exceeding 50 mm 2 and a depth of about 1 mm. Put 0.020 mL of the composition of the example or comparative example into the small chamber of the patch test device, apply it to the normal skin of the left forearm of the subject with a low-sensitization tape, gently press it with the palm to make it evenly adhere to the skin, and at the same time mark the test site for observation. After 24 h, remove the patch test tape, and observe the skin condition at 30 min, 24 h, and 48 h respectively. And a blank control group without any substance was set up.

[0104] The skin reaction was evaluated according to the following grading criteria:

[0105]

[0106] The results showed that the scoring grades of the examples and comparative examples were all 0. The above compositions were relatively mild, safe and non-irritating, and would not cause adverse reactions to the human body.

[0107] Efficacy test

[0108] Volunteers, re - enroll the volunteers according to the safety test standards.

[0109] The Canfieldsci Skin Rapid Optical Imaging System PRIMOS is used to detect the skin wrinkle volume (nasolabial fold), wrinkle depth (forehead wrinkle), and wrinkle number (crow's feet). The smaller the result, the smaller the wrinkle volume, wrinkle depth, or wrinkle number.

[0110] Volunteers should not use any cosmetics or topical medications on their faces for 3 days before the test. On the test day, clean the face in a constant - temperature and constant - humidity room and sit quietly for 30 minutes, then measure the wrinkle volume (nasolabial fold), wrinkle depth (forehead wrinkle), and wrinkle number (crow's feet) as the original values. Use it once in the morning and once in the evening for 28 consecutive days, and then return to the laboratory for retesting. The tests for the same volunteer are completed by the same measurement personnel.

[0111] The calculation formula for the improvement rate of wrinkle volume (nasolabial fold) is:

[0112] V%=(V1 - V2) / V1×100%;

[0113] Among them, V% is the continuous improvement rate of wrinkle volume (nasolabial fold), V2 is the wrinkle volume (nasolabial fold) after continuously using the sample for 28 days, and V1 is the wrinkle volume (nasolabial fold) before using the sample.

[0114] The calculation formula for the improvement rate of wrinkle depth (forehead wrinkle) is:

[0115] D%=(D1 - D2) / D1×100%;

[0116] Among them, D% is the continuous improvement rate of wrinkle depth (forehead wrinkle), D2 is the wrinkle depth (forehead wrinkle) after continuously using the sample for 28 days, and D1 is the wrinkle depth (forehead wrinkle) before using the sample.

[0117] The calculation formula for the improvement rate of wrinkle number (crow's feet) is:

[0118] N%=(N1 - N2) / N1×100%;

[0119] Among them, N% is the continuous improvement rate of wrinkle number (crow's feet), N2 is the wrinkle number (crow's feet) after continuously using the sample for 28 days, and N1 is the wrinkle number (crow's feet) before using the sample.

[0120] In addition, for the above - mentioned volunteers, after the test, a scoring evaluation of 1 - 10 points (10 points being the full score) is given for the experience effect.

[0121] The results are shown in Table 1 respectively. Each example or comparative example uses 3 volunteers for the experiment, and the results are averaged:

[0122] Table 1. Comparison of wrinkle improvement rate and experience effect

[0123]

[0124]

[0125] As can be seen from Table 1, the anti-wrinkle composition provided in the embodiments of the present application reduces the wrinkle volume (nasolabial folds), wrinkle depth (forehead wrinkles), and wrinkle number (crow's feet) to a certain extent, while the efficacy of the composition in the comparative examples decreases significantly.

[0126] Among them, it can be seen from Comparative Examples 1-3 and Example 1 that any one of the three polypeptides has a certain anti-wrinkle effect, but the combined use of the three has a greater effect on enhancing the anti-wrinkle effect. It can be seen from Comparative Examples 4-5 and Example 1 that when all three are used simultaneously, although the anti-wrinkle effect is significantly improved compared to Comparative Examples 1-3, due to the excessive or insufficient dosage of some of the peptides, there is still room for growth in the overall effect compared to Example 1.

[0127] It can be seen from Comparative Examples 6-8 and Example 1 that after adding any one of the extract components, the anti-wrinkle effect of the composition can be significantly improved, and the combined method of Example 1 is particularly significant for enhancing the anti-wrinkle effect. It can be seen from Comparative Examples 9-11 and Example 1 that compared with adding only one of the extracts, the combination of the three improves the anti-wrinkle effect, and the specific ratio method in Example 1 has a greater improvement in the anti-wrinkle effect than other ratios.

[0128] In summary, it can be seen that there is a synergistic effect among the components of the composition of the present invention. When some components are missing or the ratio changes greatly, the efficacy of the composition decreases significantly, and the volunteers also prefer the experience effect of the products in the examples.

[0129] The above has described the present application in detail in combination with the embodiments, but the present application is not limited to the above embodiments. Within the scope of knowledge possessed by those of ordinary skill in the art, various changes can be made without departing from the purpose of the present application. In addition, without conflict, the embodiments of the present application and the features in the embodiments can be combined with each other.

Claims

1. An anti-wrinkle composition, characterized in that, It includes polypeptide liposomes, Tagetes erecta extract, Stevia rebaudiana leaf / stem extract, and Theobroma grandiflorum seed extract. The polypeptide liposomes include a lipid membrane and a polypeptide located within the lipid membrane. The polypeptide includes acetyl hexapeptide-8, copper peptide, and nonapeptide-1.

2. The anti-wrinkle composition according to claim 1, characterized in that, The mass ratio of acetyl hexapeptide-8, copper peptide, and nonapeptide-1 is 1:(0.1 - 0.5):(1 - 2).

3. The anti-wrinkle composition according to claim 1, characterized in that, The raw materials of the lipid membrane include phospholipids, ceramides, and polyethylene glycol.

4. The anti-wrinkle composition according to claim 1, wherein The mass ratio of the Tagetes erecta extract, the Stevia rebaudiana leaf / stem extract, and the Theobroma grandiflorum seed extract is 1:(0.5 - 2):(0.01 - 0.1).

5. The anti-wrinkle composition according to claim 1, characterized in that, The mass ratio of the polypeptide to the Tagetes erecta extract is 1:(1 - 1000).

6. A method for preparing the anti-wrinkle composition according to any one of claims 1 to 5, characterized in that, It includes the following steps: Prepare polypeptide liposomes; Mix the polypeptide liposomes, Tagetes erecta extract, Stevia rebaudiana leaf / stem extract, and Theobroma grandiflorum seed extract to obtain the anti-wrinkle composition.

7. The preparation method according to claim 6, characterized in that, The preparation of the polypeptide liposomes includes the following steps: Mix the raw materials of the lipid membrane to obtain an oil-phase mixture; Mix acetyl hexapeptide-8, copper peptide, nonapeptide-1, and an aqueous solvent to obtain an aqueous-phase mixture; Mix the oil-phase mixture and the aqueous-phase mixture, homogenize and emulsify to obtain the polypeptide liposomes.

8. A cosmetic, characterized in that, It includes the anti-wrinkle composition according to any one of claims 1 to 5.

9. The cosmetic according to claim 8, characterized in that, The dosage form of the cosmetic includes any one of aqueous solutions, ointments, creams, emulsions, gels, hydrogels, aerosols, sprays, and injections.

10. The cosmetic according to claim 8, characterized in that, It also includes at least one of an emulsifier and a thickener.

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