Soothing composition, application thereof and skin care product

Abstract

The invention belongs to the technical field of cosmetics, and discloses a soothing composition, application thereof and a skin care product, the soothing composition is obtained by combining extracts of radix ophiopogonis roots, dandelion and origanum leaves; the extract is obtained by performing alcohol extraction on a plant combination of radix ophiopogonis roots, dandelions and oregano leaves, wherein the weight ratio of the radix ophiopogonis roots to the dandelions to the oregano leaves in the plant combination is (2-10): (2-10): (1-8). When the three extracts are combined, the inflammation reaction can be intervened from multiple angles, so that the inflammation is relieved, and the relieving purpose is achieved; more types of free radicals can be removed through mutual cooperation of the three components, and the overall free radical removing efficiency is enhanced through the synergistic effect. In addition, the moisturizing components of the three components can complement each other to construct a more perfect moisturizing system; the three components have strong synergistic effects in anti-inflammatory, anti-oxidation and moisturizing aspects, so that a better soothing effect is realized.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and discloses a soothing composition, its uses, and skin care products. Background Art

[0002] With the changes in dietary structure, environmental pollution, and the increasing pressure of work and life, the skin is exposed to various complex and harmful environmental factors, and skin health is facing unprecedented severe challenges. Excessive exposure to ultraviolet rays can not only cause skin sunburn and tanning, but also lead to skin aging, the formation of age spots, and an increased risk of skin cancer after long-term accumulation. At the same time, with the increasingly serious air pollution, the particulate matter, chemical pollutants, etc. contained in it are easily attached to the skin surface, clogging pores and disrupting the normal metabolic function of the skin, thereby causing problems such as acne, acne, and dullness. And with the development of today's society, people's pursuit of beauty is getting higher and higher. In many occasions, they need to show their most beautiful side through makeup. However, the use of various cosmetics also poses potential threats to the skin. Many cosmetic products contain chemical components such as alcohol, fragrance, and preservatives, which may irritate the skin and cause discomfort symptoms such as skin allergies, itching, and flushing.

[0003] Therefore, cosmetics on the market often add some soothing ingredients to relieve various problems that occur to the skin. However, traditional soothing products generally have the following problems: First, the symptom relief is not deep enough. Most existing soothing cosmetics only focus on the immediate relief of surface symptoms. For example, adding cooling ingredients such as menthol can make the skin feel cool instantly when applied, and briefly relieve the burning and stinging caused by sunburn and allergies. However, this method only numbs the skin nerve endings and does not repair the root cause of the damaged skin. Second, the formulations of many soothing products are unreasonable, and the components cannot work synergistically, and can only achieve a single effect.

[0004] Based on this, the technical problem to be solved by the present invention is how to prepare a soothing product with high-efficiency soothing efficacy. Summary of the Invention

[0005] The purpose of the present invention is to provide a soothing composition containing extracts of Ophiopogon japonicus roots, Taraxacum mongolicum extracts, and Origanum vulgare leaf extracts. When the three components are used together, there is a synergistic effect and excellent soothing effect.

[0006] At the same time, the present invention also provides uses of a soothing composition and skin care products.

[0007] To achieve the above purpose, the present invention provides the following technical solutions:

[0008] A soothing composition, which is obtained by combining extracts of Ophiopogon japonicus roots, dandelions and oregano leaves. The extracts are obtained by alcohol extraction from a plant combination of Ophiopogon japonicus roots, dandelions and oregano leaves. The weight ratio of Ophiopogon japonicus roots, dandelions and oregano leaves in the plant combination is 2-10:2-10:1-8.

[0009] The substances used in the present invention have the following effects respectively:

[0010] Ophiopogon japonicus root extract

[0011] In terms of antioxidant: The Ophiopogon japonicus root extract contains antioxidant components such as flavonoids and saponins. These components can react with free radicals through their phenolic hydroxyl groups to generate relatively stable phenoxy radicals, preventing the further progress of the free radical chain reaction, reducing the oxidative stress damage of the skin, alleviating skin inflammation and discomfort caused by oxidative damage, and playing a role in soothing the skin. In addition, the Ophiopogon japonicus root extract can regulate the antioxidant enzyme system in the skin, promote the synthesis of antioxidant enzymes or enhance their activity, help maintain the redox balance in skin cells, and reduce skin inflammatory reactions caused by redox imbalance, achieving the effect of soothing the skin.

[0012] In terms of anti-inflammatory: The Ophiopogon japonicus root extract can inhibit the activation of inflammatory cells, thereby reducing the release of inflammatory mediators. Some components in the Ophiopogon japonicus root extract may act on the signal pathway of mast cells, prevent their degranulation process, reduce the release amount of histamine, and thus alleviate skin allergy and inflammation symptoms, playing a soothing role. At the same time, the Ophiopogon japonicus root extract can regulate the function of immune cells, inhibit the inflammatory reaction by regulating the secretion of cytokines, and play a role in soothing the skin.

[0013] Dandelion extract

[0014] In terms of antioxidant: The polyphenols and flavonoids in the dandelion extract are powerful free radical scavengers. These compounds have multiple phenolic hydroxyl groups, and the hydrogen atoms on the phenolic hydroxyl groups can be easily taken away by free radicals. Among them, in the face of superoxide anion radicals (O2 - ) and hydroxyl radicals (·OH), the hydrogen atoms in the polyphenol compounds will combine with the free radicals to form relatively stable free radical intermediates by themselves. Due to the existence of conjugated structures such as benzene rings in this intermediate, the energy of the unpaired electrons can be dispersed through resonance and other ways, increasing its stability, thereby preventing the free radical chain reaction. The flavonoid free radicals can carry out electron delocalization through intramolecular hydrogen bonds and conjugated systems, improving their stability, and then effectively scavenging free radicals, reducing the oxidative damage of free radicals to skin cells, and achieving the soothing effect.

[0015] In terms of anti - inflammation: Dandelion extract contains rich flavonoid compounds, which can inhibit key enzymes in the inflammatory response. Dandelion extract can also inhibit the synthesis of nitric oxide (NO). In inflammatory cells (such as macrophages), inducible nitric oxide synthase (iNOS) will be activated under inflammatory stimuli, producing a large amount of NO. NO has functions such as dilating blood vessels, increasing vascular permeability, and participating in inflammatory cytotoxicity. Dandelion extract may reduce the synthesis of NO by inhibiting the expression of the iNOS gene or interfering with its enzyme activity, thereby alleviating the inflammatory response. Dandelion extract can also regulate the function of eosinophils. In allergic inflammation and some skin inflammatory diseases, eosinophils will be recruited to the inflammatory site and release a variety of toxic proteins and inflammatory mediators. Dandelion extract may inhibit the activation of eosinophils and the release of inflammatory mediators by regulating the expression of cell - surface receptors or intracellular signal transduction, thus alleviating skin inflammation.

[0016] Oregano leaf extract

[0017] The polysaccharide component in oregano leaf extract plays a role in skin moisturization. These polysaccharide molecules have a large number of hydroxyl groups, which can form hydrogen bonds with water molecules. When applied to the skin surface, the polysaccharide molecules are intertwined to form a moisturizing film on the surface of the stratum corneum of the skin. This moisturizing film can prevent the loss of water inside the skin and reduce transepidermal water loss (TEWL). Moreover, this moisturizing film can also act as a physical barrier to resist the irritation of external environmental factors (such as dust, microorganisms, etc.) to the skin. At the same time, the hydrophilicity of the polysaccharide enables it to absorb water from the surrounding environment, supplement the water on the skin surface, and keep the skin in a moist state. In addition, oregano leaf extract can also improve the arrangement structure of stratum corneum cells. Normally, the tight and orderly arrangement of stratum corneum cells is beneficial for retaining water. Oregano leaf extract may make the stratum corneum cells arrange more tightly by regulating the lipid components between cells or the expression of cell - junction proteins, enhancing the water - retaining ability of the stratum corneum, so as to relieve discomfort symptoms such as skin dryness and tightness.

[0018] When Ophiopogon japonicus root extract, Taraxacum mongolicum extract, and Origanum vulgare leaf extract are used simultaneously, there is a synergistic effect. It is speculated that the mechanism may be as follows: In terms of anti-inflammation, Ophiopogon japonicus root extract mainly anti-inflames by regulating the functions of immune cells, such as affecting T lymphocyte differentiation and Langerhans cell antigen presentation. Taraxacum mongolicum extract focuses on inhibiting the synthesis of inflammatory mediators (such as inhibiting enzymes related to the arachidonic acid metabolic pathway) and the activation of inflammatory cells (such as inhibiting mast cell degranulation). Origanum vulgare leaf extract reduces inflammation by inhibiting the activities of key enzymes such as cyclooxygenase-2, lipoxygenase, and nitric oxide synthase with phenolic compounds. When the three are combined, they can intervene in the inflammatory response from multiple perspectives. In addition, Ophiopogon japonicus root extract may inhibit the overexpression of inflammatory cytokines by regulating cytokine signaling, Taraxacum mongolicum extract can inhibit inflammatory-related signaling pathways such as NF-κB, and Origanum vulgare leaf extract can also regulate the NF-κB signaling pathway to reduce the production of inflammatory mediators. When they act together, the regulation of these signaling pathways can produce a synergistic effect.

[0019] In terms of antioxidant, the components of Ophiopogon japonicus root extract may have an advantage in scavenging a certain type of free radical (such as superoxide anion radical), the components of Taraxacum mongolicum extract have a better effect on scavenging hydroxyl radicals, and the components of Origanum vulgare leaf extract are good at scavenging lipid peroxidation free radicals. When they coexist, they can scavenge more types of free radicals and enhance the overall free radical scavenging efficiency through synergistic effects. They may cooperate with each other to form a relay-style free radical scavenging mode. The relatively stable intermediate products generated after one component scavenges free radicals can be further processed by another component, thereby more effectively interrupting the free radical chain reaction.

[0020] In terms of moisturizing, Ophiopogon japonicus root extract contains components such as polysaccharides, which form a gel-like substance by binding water molecules to form a moisturizing film on the skin surface. The polysaccharide components of Taraxacum mongolicum extract can adsorb and retain water, form a moisturizing film in the stratum corneum of the skin, and also promote the synthesis and distribution of natural moisturizing factor (NMF) in stratum corneum cells. The polysaccharide components of Origanum vulgare leaf extract also form a moisturizing film on the surface of the stratum corneum of the skin by forming hydrogen bonds with water molecules and regulate the metabolism of stratum corneum cells to promote the expression of NMF-related genes. The moisturizing components of the three can complement each other to build a more perfect moisturizing system. The gel-like moisturizing film formed by Ophiopogon japonicus root extract can provide a certain moisturizing basis. The polysaccharides of Taraxacum mongolicum extract further enhance the water retention ability of the moisturizing film and promote the synthesis of NMF at the same time. Origanum vulgare leaf extract plays a role in regulating the metabolism of stratum corneum cells and enhancing the barrier function of the moisturizing film, jointly reducing transepidermal water loss (TEWL) and improving the water content and hydration of the skin.

[0021] The three components have a synergistic effect in anti-inflammation, antioxidant, and moisturizing, and can achieve a better soothing effect.

[0022] Preferably, the extraction method of the extract is as follows:

[0023] Step 1: Add the plant combination of Ophiopogon japonicus roots, dandelions, and oregano leaves to water with a mass 10 times that of the plant combination, and perform cyclic extraction at 65 °C for 3 h to obtain an extract. Then, concentrate the extract to 25-45 wt% of the mass of the extract to obtain a first concentrated solution. Add 95 vol% ethanol with the same mass as the first concentrated solution to the first concentrated solution to obtain an alcohol precipitation solution;

[0024] Step 2: Then, perform centrifugal filtration, concentrate the filtrate until alcohol-free to obtain a second concentrated solution. Add butylene glycol and water to the second concentrated solution, stir and extract for 0.5 h, and perform ultrafiltration to obtain an ultrafiltrate, where the weight ratio of the concentrated solution, butylene glycol, and water is 3-5:3:2-4;

[0025] Step 3: Add 0.5 wt% of 1,2-hexanediol and 0.5 wt% of p-hydroxyacetophenone based on the weight of the filtrate to the filtrate, and sterilize at 85 °C for 1 h to obtain a soothing composition.

[0026] In addition, the present invention also discloses the use of the above-mentioned soothing composition in preparing skin care products.

[0027] Preferably, the skin care product is a skin care product for soothing.

[0028] Finally, the present invention also discloses a skin care product containing the above-mentioned soothing composition.

[0029] Preferably, the content of the soothing composition in the skin care product is 0.1-30 wt%.

[0030] Preferably, the dosage form of the skin care product is lotion, cream, spray, essence, lotion, facial mask, or gel.

[0031] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0032] The soothing composition of the present invention contains Ophiopogon japonicus root extract, dandelion extract, and oregano leaf extract. When the three are combined, they can intervene in the inflammatory response from multiple perspectives to reduce inflammation and achieve the soothing purpose; at the same time, the three can also cooperate with each other to scavenge more types of free radicals and enhance the overall free radical scavenging efficiency through synergistic effects. In addition, the moisturizing components of the three can complement each other to build a more perfect moisturizing system; the three components have obvious synergistic effects in anti-inflammation, antioxidant, and moisturizing, thereby achieving a better soothing effect. Description of the Drawings

[0033] Figure 1 It is a view of the chicken embryo before using the sample in Example 1;

[0034] Figure 2Observation diagram of chicken embryos after using the sample of Example 1. Detailed implementation mode

[0035] Next, in combination with the embodiments of the present invention, the technical solutions of the present invention will be clearly and completely described. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention.

[0036] Product information:

[0037] Ophiopogon japonicus root: with a water content of 14.3%, purchased from Bozhou Feimao Pharmaceutical Co., Ltd.;

[0038] Origanum vulgare leaves: with a water content of 8.6%, purchased from Bozhou Feimao Pharmaceutical Co., Ltd.;

[0039] Dandelion: with a water content of 9.5%, purchased from Bozhou Feimao Pharmaceutical Co., Ltd.

[0040] The preparation methods of the soothing compositions of each example and comparative example are as follows:

[0041] Step 1: Add the plant combination of Ophiopogon japonicus root, dandelion and Origanum vulgare leaves to 10 times the mass of the plant combination of water, and extract at 65°C for 3 h by circulation to obtain an extract. Then concentrate the extract to 40 wt% of the mass of the extract to obtain a first concentrated solution. Add 95 vol% ethanol with the same mass as the first concentrated solution to the first concentrated solution to obtain an alcohol precipitation solution;

[0042] Step 2: Then, after centrifugation, concentrate the filtrate to alcohol-free to obtain a second concentrated solution. Add butanediol and water to the second concentrated solution, stir and extract for 0.5 h, and perform ultrafiltration to obtain an ultrafiltrate, where the weight ratio of the concentrated solution, butanediol and water is 4:3:3;

[0043] Step 3: Add 0.5 wt% of 1,2-hexanediol and 0.5 wt% of p-hydroxyacetophenone to the filtrate, and sterilize at 85°C for 1 h to obtain a soothing composition.

[0044] The proportions of the plant combinations of each example and comparative example are shown in Table 1;

[0045] Table 1 Plant combination formula table (parts by weight)

[0046] Ophiopogon japonicus root Taraxacum mongolicum Origanum vulgare leaf Example 1 6 6 3 Example 2 10 2 8 Example 3 2 10 1 Comparative Example 1 15 0 0 Comparative Example 2 0 15 0 Comparative Example 3 0 0 15 Comparative Example 4 7.5 7.5 0 Comparative Example 5 10 0 5 Comparative Example 6 0 10 5

[0047] Performance detection

[0048] Chorioallantoic membrane test of chicken embryos

[0049] 1. Test purpose and principle

[0050] The chicken embryo chorioallantoic membrane (CAM) assay is an early in vitro method for evaluating eye irritation. The chorioallantoic membrane is a respiratory membrane that surrounds the chicken embryo. This assay takes advantage of the intact, clear, and transparent vascular system of the chorioallantoic membrane in the mid-stage of incubated chicken embryos. A certain amount of the test substance is directly contacted with the chorioallantoic membrane of the chicken embryo. After a period of action, the changes in the toxicity effect indicators of the chorioallantoic membrane (such as bleeding, coagulation, and vascular lysis) are observed. These indicators reflect the changes in the morphological structure, color, and permeability of blood vessels and vascular networks, as well as the phenomena of protein denaturation in the chorioallantoic membrane and its degree of damage. Then a score is combined to evaluate the eye irritation of the test substance.

[0051] The purpose of this assay is to test the ability of the test substance to cause toxic changes in the chorioallantoic membrane of chicken embryos and to evaluate the elements and processes of the potential eye irritation of the substance being evaluated.

[0052] 2. Test Materials and Methods

[0053] 2.1 Instrumentation

[0054] Fully automatic incubator;

[0055] Stereomicroscope;

[0056] SPF chicken embryos.

[0057] 2.2 Reagents

[0058] Sodium chloride;

[0059] Sodium dodecyl sulfate (SDS).

[0060] 2.3 Incubation Conditions

[0061] Room temperature: 20 - 25 °C, relative humidity: 45 - 70%. Incubation temperature: 37.5 ± 0.5 °C, relative humidity: 55 - 70%, turntable rotation: 3 - 6 times / h. Chicken embryos at 9 days of age do not need to be rotated during incubation.

[0062] 2.4 Test Method

[0063] (1) Test Operating Procedures

[0064] For each group in this test, 6 embryos are selected. The situation of the chorioallantoic membrane is recorded with a photographing device. The composition of Example 1 is diluted with pure water to an aqueous solution with a sample concentration of 2% and then added to the chorioallantoic membrane of the chicken embryo. The time of adding the sample is recorded and the air chamber is covered with moistened plastic wrap. The chicken embryo is transferred to a constant temperature and humidity incubator for cultivation. The degree of change in each toxicity effect is observed and photographed for recording.

[0065] (2) Result Observation

[0066] Observe and record the manifestations of bleeding, coagulation and vascular lysis, and score according to their severity.

[0067] (3) Data analysis

[0068] Table 2 Scoring criteria

[0069]

[0070]

[0071] The endpoint evaluation method was used for the test, and the endpoint evaluation (ES) was calculated, with the result reserved to two decimal places; when ES ≤ 4, there is no irritation; when 4 < ES ≤ 12, there is mild irritation; when 12 < ES < 16, there is moderate irritation; when ES ≥ 16, there is strong irritation / corrosion; the score of each chicken embryo = the sum of the degrees of bleeding, coagulation and vascular lysis observed in each chicken embryo; ES = the average value of the mathematical sum obtained from 6 chicken embryos.

[0072] After testing, the composition of Example 1 is mild and non-irritating. Refer to Figure 1 、 Figure 2 for the comparison of the states of chicken embryos before and after dropping the solution prepared from the composition of Example 1 onto the chicken embryos. Among them, Figure 1 is the observation diagram of the chicken embryo before using the sample, Figure 2 is the observation diagram of the chicken embryo after using the sample.

[0073] Hyaluronidase inhibition rate

[0074] 1. Test purpose and principle

[0075] Hyaluronidase is a specific lyase of hyaluronic acid, a participant in allergic reactions, and has a strong correlation with the release of histamine by mast cells. Whether the test sample has a soothing effect can be judged by the hyaluronidase inhibition rate. The higher the hyaluronidase inhibition rate, the stronger the soothing effect of the substance, and vice versa.

[0076] In this test, the hyaluronidase inhibition rate test results of the test sample and the negative control were compared. If the inhibition rate of the test sample is higher than that of the negative control, it can be considered that the test sample has a certain soothing effect.

[0077] 2. Test materials and methods

[0078] 2.1 Instrument and equipment

[0079] BSA224S analytical balance;

[0080] L6s ultraviolet spectrophotometer.

[0081] 2.2 Reagents

[0082] Hyaluronidase, purity is BR;

[0083] Sodium hyaluronate, with a purity of BR.

[0084] 2.3 Test methods

[0085] (1) Treatment of the control and test samples

[0086] Sample group: Dilute the compositions of Examples 1 to 3 and Comparative Examples 1 to 6 with pure water to a sample concentration of 5%;

[0087] Positive control (dipotassium glycyrrhizinate, purity ≥ 98%): Dilute with water to a positive control concentration of 3%;

[0088] Negative control: Pure water.

[0089] (2) Test operation steps

[0090] Set up a sample group, a sample background group, a solvent group, and a solvent background group. Three parallels should be set up for each group. Add different reagent solutions to the four groups respectively, shake well, allow to develop color at room temperature for 30 min, and measure the absorbance value at a wavelength of 528 nm using an ultraviolet spectrophotometer.

[0091] (3) Calculation formula

[0092]

[0093] In the formula:

[0094] A—Absorbance of the reaction solution without the sample and containing the enzyme; B—Absorbance of the reaction solution without the sample and the enzyme;

[0095] C—Absorbance of the reaction solution containing the sample and the enzyme; D—Absorbance of the reaction solution containing the sample and without the enzyme.

[0096] 3 Test results

[0097] Table 3 Results table of hyaluronidase inhibition rate

[0098]

[0099]

[0100] Note: The hyaluronidase inhibition rate is reserved to two decimal places.

[0101] It can be seen from the data in Table 3 that:

[0102] The solution of Example 1 of the present invention contains extracts of Ophiopogon japonicus roots, dandelion extracts, and oregano leaf extracts at the same time, and has an extremely high hyaluronidase inhibition rate of 61.07%, which is only about 7% different from the positive control group; Examples 1 to 3 are all within the dosage range of the technical solution of the present invention, and all have a relatively high hyaluronidase inhibition rate.

[0103] Comparative Examples 1 to 3 are cases where the three components are used alone, and among them, the hyaluronidase inhibition rate of the extract of Ophiopogon japonicus roots is relatively high; by comparing the data of Example 1 and Comparative Examples 1 to 3, it can be seen that in this case, by compounding the extract of Ophiopogon japonicus roots, dandelion extracts, and oregano leaf extracts, a synergistic effect of improving the hyaluronidase inhibition rate of the composition is produced.

[0104] Comparative Examples 4 to 6 are cases where the three components are combined in pairs. By analyzing their data, it is found that the dosage of Ophiopogon japonicus roots in Comparative Example 4 and Comparative Example 5 is significantly higher than that in Example 1. Theoretically, its hyaluronidase inhibition rate should be higher than that in Example 1, but in fact, the hyaluronidase inhibition rate it shows is significantly lower than that in Example 1, indicating that in this case, by compounding the extract of Ophiopogon japonicus roots, dandelion extracts, and oregano leaf extracts, a synergistic effect of improving the hyaluronidase inhibition rate of the composition is produced.

[0105] Comparative Example 6 uses a combination of dandelion extracts and oregano leaf extracts. In the absence of the extract of Ophiopogon japonicus roots, the effect it shows is only a simple addition of effects.

[0106] Macrophages detect the content of inflammatory factor PGE2

[0107] 1. Test purpose and principle

[0108] Macrophages can be used as a cell model for studying the inhibition of PGE2 content by cosmetics. By stimulating with lipopolysaccharide (LPS) of bacteria, the content of PGE2 in the negative control and the test sample group is measured to evaluate whether the test sample has an effect in inhibiting the content of PGE2. The content of PGE2 is measured by the enzyme-linked immunosorbent assay (ELISA).

[0109] In this test, referring to the laboratory method, the cell PGE2 content measurement results of the test sample and the negative control are compared. If the cell PGE2 content of the test sample is lower than that of the negative control, it can be considered that the test sample has a certain soothing effect.

[0110] 2. Test materials and methods

[0111] 2.1 Instrument and equipment

[0112] RT-6100 microplate reader.

[0113] 2.2 Cells used in the experiment

[0114] Macrophages: RAW264.7 cells.

[0115] 2.3 Test methods

[0116] (1) Treatment of test samples

[0117] Sample group: Dilute the compositions of Examples 1 - 3 and Comparative Examples 1 - 6 with pure water to a 0.5% solution, then filter through a 0.22 μm filter, and collect the filtrate as the sample.

[0118] Negative control: Basal culture medium (cell viability test); Basal culture medium containing LPS (PGE2 content determination).

[0119] (2) Test operation steps

[0120] Determination of PGE2 content: Seed RAW264.7 cells in a 96 - well plate. After culturing for 24 h, replace the medium with basal medium containing test samples at different concentrations. After 24 h, collect the cell supernatant and centrifuge. Pipette the supernatant and use an ELISA kit to determine the PGE2 content in the cell culture supernatant, so as to obtain the effect of the test samples on the PGE2 content of macrophages.

[0121] (3) Calculation formula

[0122]

[0123] 3. Test results

[0124] Table 4 PGE2 relative content data

[0125] Sample Cell viability of 0.5 wt% solution / % Relative content of PGE2 / % Example 1 97.43 51.35 Example 2 98.43 52.73 Example 3 95.45 55.36 Comparative Example 1 97.31 65.75 Comparative Example 2 95.79 79.30 Comparative Example 3 97.76 72.08 Comparative Example 4 99.07 64.64 Comparative Example 5 95.73 66.89 Comparative Example 6 95.96 71.48 Negative control 100.00 100.00

[0126] The solution of Example 1 of the present invention contains Ophiopogon japonicus root extract, Taraxacum mongolicum extract and Origanum vulgare leaf extract at the same time, and has an extremely high inhibitory effect on the PGE2 inflammatory factor. The relative content of the PGE2 inflammatory factor in the cells is 51.35%; Examples 1 - 3 are all within the dosage range of the technical solution of the present invention, and all have a high effect of inhibiting the release of the PGE2 inflammatory factor.

[0127] Comparative Examples 1 - 3 are the cases of using the three components alone. Among them, Ophiopogon japonicus root extract has a relatively high effect on inhibiting the release of the PGE2 inflammatory factor; by comparing the data of Example 1 and Comparative Examples 1 - 3, it can be seen that in this case, by compounding Ophiopogon japonicus root extract, Taraxacum mongolicum extract and Origanum vulgare leaf extract, a synergistic effect of inhibiting the release of the PGE2 inflammatory factor is produced.

[0128] Comparative Examples 4 to 6 are cases of pairwise combinations of three components. Through analysis of their data, it is found that in Comparative Example 4 and Comparative Example 5, the dosage of Ophiopogon japonicus roots is significantly higher than that in Example 1. Theoretically, the relative content of PGE2 inflammatory factor in its cells should be lower than that in Example 1, but in fact, the relative content of PGE2 inflammatory factor in its cells is significantly higher than that in Example 1, indicating that through the compounding of Ophiopogon japonicus root extract, Taraxacum mongolicum extract, and Origanum vulgare leaf extract in this case, a synergistic effect of inhibiting the release of PGE2 inflammatory factor is produced.

[0129] In Comparative Example 6, a combination of Taraxacum mongolicum extract and Origanum vulgare leaf extract was used. In the absence of Ophiopogon japonicus root extract, the effect shown was merely a simple additive effect.

[0130] Application Example

[0131] The present invention discloses a skin care product containing the soothing composition of Example 1, and its formula is shown in Table 5; the preparation method is as follows:

[0132] Step 1: Pre-dissolve Phase B and stir to mix evenly; pre-mix and stir Phase C evenly for standby.

[0133] Step 2: Add the pure water in Phase A to the pot, start homogenization, and sequentially add the remaining raw materials in Phase A under the homogenization state. Homogenize at medium speed for 30 - 60S, stir and heat to 80 - 85°C, homogenize at medium speed for 1 - 2 min until there are no gel particles, keep warm for 30 min, and then stir and cool down.

[0134] Step 3: Cool down to 50°C, and sequentially add the pre-prepared Phase B and Phase C, and stir evenly.

[0135] Step 4: Cool down to 45°C, add Phase D, and stir evenly.

[0136] Table 5 Skin Care Product Formula Table

[0137]

[0138] For those skilled in the art, it is obvious that the present invention is not limited to the details of the above exemplary embodiments, and the present invention can be implemented in other specific forms without departing from the spirit or basic characteristics of the present invention. Therefore, from any point of view, the embodiments should be regarded as exemplary and non-limiting. The scope of the present invention is defined by the appended claims rather than the above description. Therefore, all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be encompassed within the present invention. Any reference signs in the claims should not be regarded as limiting the claimed rights.

Claims

1. A soothing composition, characterized in that, The described soothing composition is obtained by combining extracts of Ophiopogon japonicus roots, dandelions, and oregano leaves. The extracts are obtained by alcohol extraction from a plant combination of Ophiopogon japonicus roots, dandelions, and oregano leaves. The weight ratio of Ophiopogon japonicus roots, dandelions, and oregano leaves in the plant combination is 2 - 10:2 - 10:1 - 8.

2. The soothing composition according to claim 1, wherein The extraction method of the described extracts is as follows: Step 1: Add the plant combination of Ophiopogon japonicus roots, dandelions, and oregano leaves to water, and perform cyclic extraction to obtain an extract solution. Then concentrate the extract solution to obtain a first concentrated solution. Add ethanol with the same mass as the first concentrated solution to the first concentrated solution to obtain an alcohol precipitation solution; Step 2: Then, through centrifugal filtration, concentrate the filtrate until there is no alcohol to obtain a second concentrated solution. Add butylene glycol and water to the second concentrated solution, stir and extract, and perform ultrafiltration to obtain an ultrafiltrate. The weight ratio of the concentrated solution, butylene glycol, and water is 3 - 5:3:2 - 4; Step 3: Add a preservative to the ultrafiltrate, and after sterilization, obtain the soothing composition.

3. Use of the soothing composition according to any one of claims 1 - 2 in the preparation of skin care products.

4. The use according to claim 3, characterized in that, The skin care products are skin care products for soothing.

5. A skin care product, characterized in that, Contain the soothing composition according to any one of claims 1 - 2.

6. The skin care product according to claim 5, wherein, The content of the soothing composition in the skin care products is 0.1 - 30 wt%.

7. The skin care product according to claim 6, characterized in that, The dosage form of the skin care products is lotion, cream, spray, essence, lotion, mask, gel.

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