Pseudomonas for degrading acyclovir and application thereof

Abstract

The invention discloses pseudomonas for degrading acyclovir and application of the pseudomonas, and relates to the technical field of microorganisms, the pseudomonas is named as pseudomonas CHJ605 (Pseudomonas protegens CHJ605), and is preserved in the China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC NO: M20242157, and the preservation time is October 10, 2024. The pseudomonas CHJ605 provided by the invention can efficiently degrade the acyclovir in the sewage at room temperature under aerobic conditions, and can completely degrade the acyclovir in the sewage with the acyclovir concentration of 1mM within 96 hours under the inoculum size of 1%-3%.

Description

Technical Field

[0001] The present invention relates to the field of microbial technology, and particularly relates to a Pseudomonas bacterium capable of degrading acyclovir and its application. Background Art

[0002] Acyclovir, also known as Acycloguanosine, is an antiviral drug of synthetic purine nucleoside analogs, mainly used for various infections caused by herpes viruses and is the first choice for the treatment of herpes simplex virus encephalitis. It is a white to off-white crystalline powder with a molecular weight of 225.21 and a solubility in water of less than 2.5 mg / mL at 37°C. The bioavailability of acyclovir is very low, and 70%-85% of the original drug is excreted in the urine after administration. Although acyclovir can be biodegraded in sewage treatment plants, the carboxy-acyclovir generated by biotransformation is more stable than the parent structure and is difficult to be secondarily degraded in the environment.

[0003] The discharge of antiviral drugs such as acyclovir into the environment causes certain harm to the environment, mainly being dangerous and toxic to biological groups such as crustaceans, fish, and algae. The degradation research of acyclovir mainly focuses on photocatalysis and advanced oxidation, and there is research on biodegradation of this compound by activated sludge, but there is no report on the degradation of this compound by pure culture microorganisms. Summary of the Invention

[0004] The main object of the present invention is to propose a Pseudomonas bacterium capable of degrading acyclovir and its application, aiming to propose a microorganism capable of degrading acyclovir.

[0005] To achieve the above object, the present invention proposes a Pseudomonas bacterium capable of degrading acyclovir, named Pseudomonas protegens CHJ605, which is preserved in the China Center for Type Culture Collection, with the preservation number of CCTCC NO: M 20242157, and the preservation time is October 10, 2024.

[0006] The present invention also proposes a screening method for the Pseudomonas bacterium capable of degrading acyclovir as described above, including the following steps:

[0007] S10. Obtain activated sludge, inoculate the activated sludge into a liquid inorganic salt medium, supplement acyclovir, and after culturing on a shaker for 7 to 10 days, take the culture and inoculate it into a new liquid inorganic salt medium, and add acyclovir for subculture;

[0008] S20. Dilute the culture after 5 to 6 subcultures into a diluted bacterial solution, take the diluted bacterial solution and spread it on an inorganic salt solid medium plate, add acyclovir, and culture it in an incubator at 28°C to 30°C for 7 to 10 days;

[0009] S30. Pick the larger single colonies on the inorganic salt solid medium, and after separation and purification, obtain the screened strains;

[0010] S40. Inoculate the screened strains into a new liquid inorganic salt medium, add acyclovir, and then culture them on a shaker for 40 - 48 hours. Measure the content of acyclovir in the medium, and screen out the strain with the most significant reduction in the acyclovir content in the medium, which is the Pseudomonas aeruginosa that degrades acyclovir.

[0011] In one embodiment, the liquid inorganic salt medium comprises components with the following concentrations:

[0012] Na2HPO4·12H2O 10.2 - 14.3 g / L, KH2PO4 2 - 3 g / L, FeSO4·7H2O 0.2 - 0.3 mg / L, MnSO4·H2O 0.05 - 0.07 mg / L, MgSO4·7H2O 0.015 - 0.02 mg / L, CaCl2 0.25 - 0.3 mg / L, CuSO4 0.0125 - 0.2 mg / L, ZnSO4 0.0125 - 0.2 mg / L, and H3BO3 0.0125 - 0.2 mg / L, (NH4)2SO4 2 - 3 mM;

[0013] The pH value of the liquid inorganic salt medium is 7.0 - 7.5;

[0014] The inorganic salt solid medium is the medium obtained by adding 15 - 20 g / L of agar powder to the liquid inorganic salt medium.

[0015] In one embodiment, in step S10:

[0016] The temperature of the shaker culture is 28°C - 30°C, and the shaker speed is 180 - 200 r / min;

[0017] The concentration of acyclovir is 2 - 3 mM;

[0018] The mass concentration of the activated sludge in the liquid inorganic salt medium is 10 - 50 g / L;

[0019] In step S20, the concentration of acyclovir is 2 - 3 mM.

[0020] The present invention also provides an application of the Pseudomonas aeruginosa that degrades acyclovir as described above in treating sewage, where the sewage contains acyclovir, and the Pseudomonas aeruginosa is used to reduce the content of acyclovir in the sewage.

[0021] The present invention also provides an application of the Pseudomonas aeruginosa that degrades acyclovir as described above in the preparation of biological agents.

[0022] The present invention also provides a biological bactericide for degrading acyclovir, which comprises the aforementioned Pseudomonas for degrading acyclovir.

[0023] The present invention also provides a method for degrading acyclovir in sewage, comprising the following steps:

[0024] Inoculate the aforementioned Pseudomonas into the sewage containing acyclovir, and supplement inorganic salts, and degrade for 80 - 120 hours.

[0025] In one embodiment, the inoculation amount of the Pseudomonas in the sewage is 1% - 3%.

[0026] The Pseudomonas CHJ605 provided by the present invention can efficiently degrade acyclovir in sewage at room temperature and under aerobic conditions. At an inoculation amount of 1% - 3%, it can completely degrade acyclovir in sewage with an acyclovir concentration of 1 mM within 96 hours. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the following drawings are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other related drawings can also be obtained based on these drawings.

[0028] Figure 1 It is the Gram staining diagram of strain CHJ605 in Embodiment 1 of the present invention;

[0029] Figure 2 It is the colony morphology diagram of strain CHJ605 in Embodiment 1 of the present invention;

[0030] Figure 3 It is the diagram of the degradation of acyclovir by strain CHJ605 and its growth result in Embodiment 2 of the present invention.

[0031] The realization of the object, functional features and advantages of the present invention will be further described in conjunction with the embodiments with reference to the drawings. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0032] To make the objectives, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. For those conditions not specified in the embodiments, they are carried out according to conventional conditions or the conditions recommended by the manufacturer. For the reagents or instruments not specified by the manufacturer, they are all conventional products that can be obtained through commercial purchase.

[0033] Currently, the degradation research of acyclovir mainly focuses on photocatalysis and advanced oxidation, and there is research on biodegradation of this compound by activated sludge, but there is no report on the biodegradation of this compound by pure-culture microorganisms.

[0034] In view of this, the present invention provides a Pseudomonas strain for degrading acyclovir, named Pseudomonas protegens CHJ605, which is deposited in the China Center for Type Culture Collection (CCTCC), with the deposit address being Wuhan, China, the deposit number being CCTCC NO: M20242157, and the deposit date being October 10, 2024.

[0035] The Pseudomonas protegens CHJ605 provided by the present invention can efficiently degrade acyclovir in sewage at room temperature and aerobic conditions. At an inoculation amount of 1% - 3%, it can completely degrade acyclovir in sewage with an acyclovir concentration of 1 mM within 96 hours.

[0036] The present invention also provides a screening method for the Pseudomonas strain for degrading acyclovir as described above, comprising the following steps:

[0037] S10. Obtain activated sludge, inoculate the activated sludge into a liquid inorganic salt medium, add acyclovir, and culture it on a shaker for 7 - 10 days. Then, take the culture and inoculate it into a new liquid inorganic salt medium, and add acyclovir for subculture.

[0038] S20. Dilute the culture after 5 - 6 subcultures into a diluted bacterial solution, take the diluted bacterial solution and spread it on an inorganic salt solid medium plate, add acyclovir, and culture it in an incubator at 28°C - 30°C for 7 - 10 days.

[0039] S30. Pick the larger single colonies on the inorganic salt solid medium, and after separation and purification, obtain the screened strains.

[0040] S40. Inoculate the screened strains into a new liquid inorganic salt medium, add acyclovir, and culture it on a shaker for 40 - 48 hours. Measure the content of acyclovir in the medium, and screen out the strain with the most significant reduction in the acyclovir content in the medium, which is the Pseudomonas strain for degrading acyclovir.

[0041] In the technical solution of the present invention, in step S10, the activated sludge is sampled at multiple points from the activated sludge of a Wuhan sewage treatment plant as the raw material for screening strains that can efficiently degrade acyclovir. It should be noted that when inoculating the culture into a new liquid inorganic salt medium, all or part of the culture can be selected according to actual needs. In this embodiment, only part of the culture is inoculated into the new liquid inorganic salt medium. Specifically, in order to provide sufficient growth space and nutrients for the microorganisms in the activated sludge, the mass ratio of the activated sludge to the volume of the liquid inorganic salt medium is 1 g: 20 - 100 mL.

[0042] In one embodiment, the liquid inorganic salt medium comprises components with the following concentrations:

[0043] Na2HPO4·12H2O 10.2 - 14.3 g / L, KH2PO4 2 - 3 g / L, FeSO4·7H2O 0.2 - 0.3 mg / L, MnSO4·H2O 0.05 - 0.07 mg / L, MgSO4·7H2O 0.015 - 0.02 mg / L, CaCl2 0.25 - 0.3 mg / L, CuSO4 0.0125 - 0.2 mg / L, ZnSO4 0.0125 - 0.2 mg / L, and H3BO3 0.0125 - 0.2 mg / L, (NH4)2SO4 2 - 3 mM;

[0044] The pH value of the liquid inorganic salt medium is 7.0 - 7.5;

[0045] The inorganic salt solid medium is obtained by adding 15 - 20 g / L of agar powder to the liquid inorganic salt medium.

[0046] In one embodiment, in step S10:

[0047] The temperature for shaking culture is 28°C - 30°C, and the shaking speed is 180 - 200 r / min;

[0048] The concentration of acyclovir is 2 - 3 mM;

[0049] The mass concentration of the activated sludge in the liquid inorganic salt medium is 10 - 50 g / L;

[0050] In step S20, the concentration of acyclovir is 2 - 3 mM.

[0051] The present invention also proposes an application of the Pseudomonas aeruginosa for degrading acyclovir as described above in treating sewage, where the sewage contains acyclovir, and the Pseudomonas aeruginosa is used to reduce the content of acyclovir in the sewage.

[0052] The present invention also provides an application of the aforementioned Pseudomonas in preparing a biological bacterial agent for degrading acyclovir.

[0053] The present invention also provides a biological bacterial agent for degrading acyclovir, which comprises the aforementioned Pseudomonas for degrading acyclovir.

[0054] The present invention also provides a method for degrading acyclovir in sewage, comprising the following steps:

[0055] Inoculate the aforementioned Pseudomonas into sewage containing acyclovir, supplement inorganic salts, and degrade for 80 - 120 h.

[0056] In the technical solution of the present invention, the Pseudomonas CHJ605 can efficiently degrade acyclovir in sewage at room temperature and under aerobic conditions. For sewage with an acyclovir concentration of 1 mM, acyclovir can be completely degraded within 96 hours.

[0057] It should be noted that whether during the process of screening the strain for highly efficient degradation of acyclovir or after the screening is completed, when detecting the degradation effect of the strain for highly efficient degradation of acyclovir on acyclovir, qualitative and quantitative analysis of acyclovir is required. There are many detection methods for measuring the qualitative and quantitative of acyclovir. Considering the specific situation in the experimental process of the present invention, the high performance liquid chromatography (HPLC) detection method of acyclovir is preferably used to analyze the changes of acyclovir during the experiment.

[0058] Among them, the necessary inorganic salt components are preferably the same as those of the liquid inorganic salt medium described above in this article to provide necessary nutrients for the growth and reproduction of the Pseudomonas CHJ605.

[0059] In one embodiment, the inoculation amount of the Pseudomonas in the sewage is 1% - 3%.

[0060] In the technical solution of the present invention, by adjusting the inoculation amount of the Pseudomonas in the sewage to 1% - 3%, it can ensure that the Pseudomonas effectively degrades acyclovir in the sewage; if the inoculation amount is less than 1%, the degradation time is too long and the efficiency is too low; if the inoculation amount is higher than 3%, the number of Pseudomonas is large, and inorganic salts or oxygen are easily consumed rapidly during the growth process, resulting in the death of Pseudomonas.

[0061] The following further details the technical solution of the present invention in conjunction with specific embodiments and the accompanying drawings. It should be understood that the following embodiments are only used to explain the present invention and are not used to limit the present invention.

[0062] Experimental materials

[0063] The activated sludge for experiments was sampled from multiple points in the activated sludge of a Wuhan sewage treatment plant;

[0064] Liquid inorganic salt medium: Na2HPO4·12H2O 10.2 - 14.3 g / L, KH2PO4 2 - 3 g / L, FeSO4·7H2O 0.2 - 0.3 mg / L, MnSO4·H2O 0.05 - 0.07 mg / L, MgSO4·7H2O 0.015 - 0.02 mg / L, CaCl2 0.25 - 0.3 mg / L, CuSO4 0.0125 - 0.2 mg / L, ZnSO4 0.0125 - 0.2 mg / L, and H3BO3 0.0125 - 0.2 mg / L, (NH4)2SO4 2 - 3 mM;

[0065] Inorganic salt solid medium: obtained by solidifying the liquid inorganic salt medium with 25 g / L agar.

[0066] Example 1 Screening of strains highly efficient in degrading acyclovir

[0067] Samples were taken from multiple points in the activated sludge of a Wuhan sewage treatment plant in China. The specific screening steps are as follows:

[0068] Step S10: The activated sludge was inoculated into the liquid inorganic salt medium. The mass concentration of the activated sludge in the liquid inorganic salt medium was 30 g / L. 2 mM acyclovir was added as a supplement. After culturing on a shaker for 7 - 10 days, the culture was taken and inoculated into a new liquid inorganic salt medium, and 2 mM acyclovir was added for subculture;

[0069] Step S20: The culture after 5 - 6 subcultures was diluted into a diluted bacterial solution. The diluted bacterial solution was spread on the inorganic salt solid medium plate. After adding 2 mM acyclovir, it was cultured in an incubator at 28°C - 30°C for 7 - 10 days;

[0070] Step S30: The larger single colonies on the inorganic salt solid medium were picked. After separation and purification, the screened strains were obtained;

[0071] Step S40: The screened strains were inoculated into a new liquid inorganic salt medium, and acyclovir was added. After culturing on a shaker for 40 - 48 hours, the content of acyclovir in the medium was measured by HPLC. The strain with the most significant reduction in the acyclovir content in the medium was screened out, which was the strain highly efficient in degrading acyclovir.

[0072] Example 2 Strain identification

[0073] Traditional morphological identification

[0074] The isolated strains were subjected to Gram staining and observed under a microscope. The morphological characteristics of their colonies were observed. The morphology under a 100 - fold microscope was asFigure 1 As shown, it indicates that the obtained strain is Gram-negative.

[0075] Observation was carried out by culturing on an inorganic salt solid medium, and the results are as Figure 2 shown. After culturing Pseudomonas CHJ605 on a solid inorganic salt medium plate, the colonies are round, with relatively regular edges, milky white to light yellow, smooth and moist on the surface, and the color is light and uniform.

[0076] Molecular biological identification (identification of ribosomal 16S rDNA sequence)

[0077] Test method: By means of polymerase chain reaction (PCR), using Taq DNA polymerase, the ribosomal 16S rDNA sequence of Pseudomonas CHJ605 was amplified with primers 1492R and F27. The PCR amplification product was sent to Shanghai Sangon Biological Engineering Technology & Services Co., Ltd. for sequencing to obtain the 16S rRNA gene sequence of this strain, and the taxonomic status of this strain was determined by comparison with highly similar sequences.

[0078] The 16S rRNA gene sequence is as follows:

[0079] TTTGAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGC

[0080] AGGCCTAACACATGCAAGTCGAGCGGCAGCACGGGTACTTGTACCTGGT

[0081] GGCGAGCGGCGGACGGGTGAGTAATGCCTAGGAATCTGCCTAGTAGTGG

[0082] GGGATAACGTCCGGAAACGGGCGCTAATACCGCATACGTCCTACGGGAG

[0083] AAAGTGGGGGATCTTCGGACCTCACGCTATTAGATGAGCCTAGGTCGGA

[0084] TTAGCTAGTTGGTGAGGTAATGGCTCACCAAGGCGACGATCCGTAACTG

[0085] GTCTGAGAGGATGATCAGTCACACTGGAACTGAGACACGGTCCAGACT

[0086] CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTG

[0087] ATCCAGCCATGCCGCGTGTGTGAAGAAGGTCTTCGGATTGTAAAGCACT

[0088] TTAAGTTGGGAGGAAGGGCAGTTACCTAATACGTGATTGTTTTGACGTTA

[0089] CCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATAC

[0090] AGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGG

[0091] TGGTTTGTTAAGTTGGATGTGAAAGCCCCGGGCTCAACCTGGGAACTGC

[0092] ATCCAAAACTGGCAAGCTAGAGTATGGTAGAGGGTGGTGGAATTTCCTG

[0093] TGTAGCGGTGAAATGCGTAGATATAGGAAGGAACACCAGTGGCGAAGG

[0094] CGACCACCTGGACTGATACTGACACTGAGGTGCGAAAGCGTGGGGAGC

[0095] AAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCAACTA

[0096] GCCGTTGGGAGCCTTGAGCTCTTAGTGGCGCAGCTAACGCATTAAGTTG

[0097] ACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAATGAATTGACG

[0098] GGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCG

[0099] AAGAACCTTACCAGGCCTTGACATCCAATGAACTTTCTAGAGATAGATTG

[0100] GTGCCTTCGGGAACATTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCG

[0101] TGTCGTGAGATGTTGGGTTAAGTCCCGTAACGAGCGCAACCCTTGTCCT

[0102] TAGTTACCAGCACGTTATGGTGGGCACTCTAAGGAGACTGCCGGTGACA

[0103] AACCGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGGC

[0104] CTGGGCTACACACGTGCTACAATGGTCGGTACAAAGGGTTGCCAAGCCG

[0105] CGAGGTGGAGCTAATCCCATAAAACCGATCGTAGTCCGGATCGCAGTCT

[0106] GCAACTCGACTGCGTGAAGTCGGAATCGCTAGTAATCGCGAATCAGAAT

[0107] GTCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCA

[0108] TGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGAGGACG

[0109] GTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCC

[0110] GTAGGGGAACCTGCGGCTGGATCACCTCCTTAAT(SEQ ID NO.1). Performance determination of acyclovir degradation by Pseudomonas CHJ605 in Example 3

[0111] The Pseudomonas CHJ605 obtained by culturing in Example 1 was inoculated into a liquid inorganic salt medium containing 1 mM acyclovir, and the inoculation amount was 2%.

[0112] Step A10, Pseudomonas CHJ605 was inoculated into a liquid inorganic salt medium, 2 mM acyclovir was added, and it was cultured in a shaker at 28 °C and 200 r / min to obtain an acyclovir-degrading bacterial solution.

[0113] Step A20: At different time intervals, sample 500 L of the degrading bacterial solution, add 500 L of acetonitrile, mix evenly by shaking for 2 min, centrifuge the mixed sample at 12,000 g for 10 min, then use a disposable sterile syringe to aspirate the solution, filter it through a 0.22-μm organic filter membrane, and place it in a brown chromatographic vial to obtain a test sample.

[0114] Step A30: Detect the test sample by high performance liquid chromatography (HPLC). The HPLC uses a Dalian Elite EClassical 3100system chromatograph, the chromatographic column is a C18 column Supersil ODS2 chromatographic column (4.6 x 200 mm, 5 μm), the column temperature is 30 °C, and the injection volume is 20 L. The mobile phase is 20% water (A) and 80% acetonitrile (B), with gradient elution. The detection wavelength of the DAD detector is 275 nm. Qualitative analysis is performed by comparing the retention time with the standard product, and quantitative analysis is performed by the external standard method.

[0115] The detection method of the high performance liquid chromatography (HPLC) has high separation efficiency, good selectivity, and high detection sensitivity. It can effectively separate and detect acyclovir in the above-mentioned degrading solution, and can qualitatively analyze acyclovir and quantitatively analyze the changes in acyclovir.

[0116] The final results are as Figure 3 shown. Among them, Control Group 1 is a liquid inorganic salt medium containing 1 mM acyclovir, but Pseudomonas sp. CHJ605 is not inoculated; Control Group 2 is a liquid inorganic salt medium without added acyclovir, but Pseudomonas sp. CHJ605 is inoculated.

[0117] According to Figure 3 it can be seen that Pseudomonas sp. CHJ605 can completely degrade 1 mM of acyclovir within 96 hours. At the same time, Pseudomonas sp. CHJ605 can use acyclovir as its carbon and nitrogen source for growth and reproduction; while the acyclovir concentration in Control Group 1 has no obvious change, and the number of Pseudomonas in Control Group 2 has no obvious change, further proving that acyclovir can participate in the metabolism of Pseudomonas sp. CHJ605.

[0118] The above are only the preferred embodiments of the present invention, and do not limit the patent scope of the present invention. For those skilled in the art, the present invention can have various changes and modifications. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the patent protection scope of the present invention.

Claims

1. A Pseudomonas that degrades acyclovir, characterized in that, The Pseudomonas is named Pseudomonas protegens CHJ605, deposited in the China Center for Type Culture Collection, with the deposit number CCTCC NO: M 20242157, and the deposit date is October 10, 2024.

2. A screening method for Pseudomonas that degrades acyclovir as described in claim 1, characterized in that, It includes the following steps: S10. Obtain activated sludge, inoculate the activated sludge into a liquid inorganic salt medium, supplement acyclovir, and after culturing on a shaker for 7 - 10 days, take the culture and inoculate it into a new liquid inorganic salt medium, and add acyclovir for subculture; S20. Dilute the culture after 5 - 6 subcultures into a diluted bacterial solution, take the diluted bacterial solution and spread it on an inorganic salt solid medium plate, add acyclovir and culture it in an incubator at 28°C - 30°C for 7 - 10 days; S30. Pick the larger single colonies on the inorganic salt solid medium, and after separation and purification, obtain the screened strains; S40. Inoculate the screened strains into a new liquid inorganic salt medium, add acyclovir and culture it on a shaker for 40 - 48 hours, measure the content of acyclovir in the medium, and screen out the strain with the most reduction in the content of acyclovir in the medium, which is the Pseudomonas that degrades acyclovir.

3. The screening method of Pseudomonas for degrading acyclovir according to claim 2, characterized in that, The liquid inorganic salt medium includes components with the following concentrations: Na2HPO4·12H2O 10.2 - 14.3 g / L, KH2PO4 2 - 3 g / L, FeSO4·7H2O 0.2 - 0.3 mg / L, MnSO4·H2O 0.05 - 0.07 mg / L, MgSO4·7H2O 0.015 - 0.02 mg / L, CaCl2 0.25 - 0.3 mg / L, CuSO4 0.0125 - 0.2 mg / L, ZnSO4 0.0125 - 0.2 mg / L, and H3BO3 0.0125 - 0.2 mg / L, (NH4)2SO4 2 - 3 mM; The pH value of the liquid inorganic salt medium is 7.0 - 7.5; The inorganic salt solid medium is the medium obtained by adding 15 - 20 g / L agar powder to the liquid inorganic salt medium.

4. The screening method of Pseudomonas for degrading acyclovir according to claim 2, characterized in that, In step S10: The temperature of the shaker culture is 28°C - 30°C, and the shaker speed is 180 - 200 r / min; The concentration of acyclovir is 2 - 3 mM; The mass concentration of the activated sludge in the liquid inorganic salt medium is 10 - 50 g / L; In step S20, the concentration of acyclovir is 2 - 3 mM.

5. Use of the Pseudomonas that degrades acyclovir as described in claim 1 in treating sewage, characterized in that, The sewage contains acyclovir, and the Pseudomonas is used to reduce the content of acyclovir in the sewage.

6. Use of the Pseudomonas that degrades acyclovir as claimed in claim 1 in the preparation of a biological bacterial agent.

7. A biological agent for degrading acyclovir, characterized in that, The biological bacterial agent includes the Pseudomonas that degrades acyclovir as claimed in claim 1.

8. A method for degrading acyclovir in sewage, characterized in that, It includes the following steps: Inoculate the Pseudomonas as claimed in claim 1 into the sewage containing acyclovir, and supplement inorganic salts, and degrade for 80 - 120 h.

9. Use of the Pseudomonas that degrades acyclovir according to claim 8 in treating sewage, characterized in that, The inoculation amount of the Pseudomonas in the sewage is 1% - 3%.

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