Handheld multi-tooth PCR plate film sealing device

Abstract

The invention provides a handheld multi-tooth PCR plate film sealing device, and relates to the technical field of film sealing devices. The device comprises a main body unit, a tooth-shaped unit and a scraper blade unit. The tooth-shaped units comprise scribing structures, and the multiple scribing structures are connected with the main body unit and evenly distributed at equal intervals. A plurality of scribing structures can accurately scribe gaps in holes of the PCR plate, and pressure is effectively applied; meanwhile, when an operator uses the film sealing device for a single time, a plurality of gaps between holes can be sealed at the same time through each time of scribing, the number of times of extra scribing needed in the whole is greatly reduced, time consumed for sealing the film at each time is greatly reduced, and the film sealing efficiency is remarkably improved. And the scraper unit is connected with the main body unit. And when the scraper unit scrapes the PCR plate surface, air between the plate sealing film and the PCR plate can be exhausted. The handheld multi-tooth PCR plate film sealing device is simple in structure, can reliably and quickly attach a pressure-sensitive PCR plate sealing film to a porous PCR plate, and effectively prevents a sample from leaking.

Description

Technical Field

[0001] The present invention relates to the technical field of film sealers, and particularly to a handheld multi-tooth PCR plate film sealer. Background Art

[0002] Biological laboratories often need to use pressure-sensitive PCR sealing films to temporarily seal multi-well PCR plates, so that the samples in the wells will not accidentally leak during steps such as oscillation and centrifugation, preventing sample loss or cross-contamination.

[0003] During the process of sealing the PCR plate with the sealing film, a film sealer is usually needed to assist. After placing the sealing film on the PCR plate, the film sealer is used to scrape across the plate surface multiple times to expel air, and then the four corners of the film sealer are used to vertically scratch the sealing edge of the plate, but no additional treatment is done for the gaps between the wells. However, it is found in actual use that there may be a risk of sample leakage if the gaps between the wells are not treated, and such film sealers are relatively expensive.

[0004] Therefore, some laboratories use film sealers with thinner edges and better prices, and on the basis of the original recommended scheme, the four corners of the film sealer are used to additionally scratch and seal the gaps between the wells parallel to the long side and the short side of the PCR plate in sequence. Although this step can prevent sample leakage to a certain extent, due to the large number of gaps between the wells, it takes a significant amount of time and the efficiency is low, making experimental processes such as DNA purification that require multiple manual plate sealings inefficient, laborious, and boring. Summary of the Invention

[0005] The purpose of the present invention is to provide a handheld multi-tooth PCR plate film sealer to solve the technical problems existing in the prior art. The many technical effects that can be produced by the preferred technical solutions provided by the present invention are described in detail below.

[0006] To achieve the above purpose, the present invention provides the following technical solutions:

[0007] A handheld multi-tooth PCR plate film sealer includes a main body unit, a toothed unit, and a scraping unit. The toothed unit includes a scribing structure, and a plurality of the scribing structures are connected to the main body unit and are evenly distributed at equal intervals. The scraping unit is connected to the main body unit.

[0008] Preferably, the main body unit is a rectangular plate-like structure, a plurality of the scribing structures are connected to one end of the main body unit in the length direction, and the scraping unit is connected to one end of the main body unit in the width direction.

[0009] Preferably, the scribing structure has two first ridges symmetrically arranged along the thickness direction of the main body unit, and the scribing structure forms a prismoid symmetrically about the first ridges on the left and right.

[0010] Preferably, the toothed unit further includes an arc-shaped connecting portion. One arc-shaped connecting portion is connected between two adjacent scribing structures, and the arc-shaped connecting portion is connected to the main body unit.

[0011] Preferably, the scraping unit includes a main body portion. The main body portion is connected to the main body unit and has the same length as the main body unit. The thickness of the main body portion gradually decreases from the end close to the main body unit to the end far from the main body unit.

[0012] Preferably, the scraping unit further includes an extension portion. The extension portion is formed by the main body portion extending outward at the end close to the toothed unit in the length direction. The outer side wall of the scribing structure closest to the scraping unit in the toothed unit is connected to the extension portion.

[0013] Preferably, a support unit is further included, and the support unit is connected to the main body unit.

[0014] Preferably, the support unit includes a prismatic support structure. Each scribing structure is respectively connected to a prismatic support structure on the front side and the back side of the main body unit. The prismatic support structure is connected to the main body unit. The length direction of the prismatic support structure is parallel to the length direction of the main body unit. The prismatic support structure has a second central ridge, and the second central ridge is collinear with the corresponding first central ridge. The prismatic support structure is symmetrically formed into a triangular prism along the second central ridge.

[0015] Preferably, an auxiliary holding unit is further included, and the auxiliary holding unit is connected to the main body unit.

[0016] Preferably, the auxiliary holding unit includes a holding platform structure. Two holding platform structures are respectively connected to the front and the back of the main body unit. The top surface of the holding platform structure is flush with the ridge edge of the first central ridge or the second central ridge.

[0017] The beneficial effects of the present invention include:

[0018] The scribing structure of the toothed unit can accurately scribe the gaps in the holes of the PCR plate, effectively apply pressure, and achieve rapid sealing. At the same time, due to the provision of a plurality of scribing structures, when the operator uses the film sealer once, each scribing can seal multiple inter-hole gaps simultaneously, greatly reducing the overall number of additional scribing times required. On the premise of ensuring reliable no leakage, the operator can greatly reduce the time consumed for each film sealing, significantly improving the film sealing efficiency.

[0019] The scraping unit is connected to the main body unit. When the scraping unit scrapes across the PCR plate surface, it can discharge the air between the sealing film and the PCR plate.

[0020] The structure of the handheld multi-tooth PCR plate sealer is more concise, and it can reliably and quickly attach the pressure-sensitive PCR sealing film to the porous PCR plate, effectively preventing sample leakage. Description of the Drawings

[0021] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0022] Figure 1 It is a three-dimensional structure diagram of the present invention;

[0023] Figure 2 It is a detailed structure diagram of the present invention;

[0024] Figure 3 It is a front view structure diagram of the present invention;

[0025] Figure 4 It is a bottom view structure diagram of the present invention;

[0026] In the figure,

[0027] 1. Main body unit;

[0028] 2. Tooth-shaped unit; 21. Scoring structure; 211. First mid-ridge; 22. Arc-shaped connecting part;

[0029] 3. Scraper unit; 31. Main body part; 32. Extension part;

[0030] 4. Support unit; 41. Prismatic support structure; 411. Second mid-ridge;

[0031] 5. Auxiliary holding unit; 51. Holding platform structure. Detailed Embodiments

[0032] In order to make the purpose, technical solutions and advantages of the present invention clearer, the technical solutions of the present invention will be described in detail below. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other implementation manners obtained by those of ordinary skill in the art without creative efforts fall within the scope protected by the present invention.

[0033] In the description of the present invention, it should be understood that the orientation or positional relationships indicated by the terms "center", "lateral", "length", "width", "height", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "side", etc. are based on the Figure 1 orientation or positional relationship shown, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as a limitation to the present invention.

[0034] In the description of the present invention, it should also be noted that unless otherwise clearly specified and defined, the terms "installed", "connected", and "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0035] Referring to Figures 1 to 4 , the present invention provides a handheld multi-tooth PCR plate sealer, which includes a main body unit 1, a toothed unit 2, and a scraper unit 3;

[0036] The toothed unit 2 includes a scribing structure 21. A plurality of scribing structures 21 are connected to the main body unit 1 and are evenly distributed at equal intervals. The scribing structure 21 of the toothed unit 2 can accurately scribe the gaps in the PCR plate holes, effectively apply pressure, and achieve rapid sealing;

[0037] At the same time, due to the provision of a plurality of scribing structures 21, when the operator uses the sealer once, each scribing can seal multiple inter-hole gaps simultaneously, greatly reducing the total number of additional scribing times required. On the premise of ensuring reliable no-leakage, the operator can greatly reduce the time-consuming for each sealing, and significantly improve the sealing efficiency;

[0038] The scraper unit 3 is connected to the main body unit 1. When the scraper unit 3 scrapes across the PCR plate surface, it can discharge the air between the sealing plate film and the PCR plate;

[0039] The structure of the handheld multi-tooth PCR plate sealer is more concise, and it can reliably and quickly attach the pressure-sensitive PCR sealing plate film to the multi-well PCR plate, effectively preventing sample leakage.

[0040] In this embodiment, taking a 96-well PCR plate as an example, the operation method of the prior art requires at least 9 scribing operations parallel to the long side and 13 scribing operations parallel to the short side. By using the handheld multi-tooth PCR plate sealer mentioned in this embodiment, it can be reduced to at least 2 scribing operations parallel to the long side and 3 scribing operations parallel to the short side. Skilled operators can halve the sealing time per operation to about 15 seconds while ensuring reliable no-leakage, significantly improving the sealing efficiency.

[0041] As an optional implementation manner, the main body unit 1 is a cuboid plate-like structure, forming the plate-like foundation of the whole sealer;

[0042] A plurality of scribing structures 21 are connected to one end of the main body unit 1 in the length direction, and the scraping unit 3 is connected to one end of the main body unit 1 in the width direction.

[0043] As an optional implementation manner, the scribing structure 21 has two first ridges 211 symmetrically arranged along the thickness direction of the main body unit 1. The scribing structure 21 is symmetrically formed into a prismoid along the first ridges 211. The prismoid can accurately scribe the gap in the PCR plate hole, effectively apply pressure, and achieve rapid sealing.

[0044] As an optional implementation manner, the toothed unit 2 further includes an arc connecting portion 22. An arc connecting portion 22 is connected between two adjacent scribing structures 21. The arc connecting portion 22 is connected to the main body unit 1. The arc connecting portion 22 can strengthen the structural strength at the scribing structure 21 and improve the connection strength between the main body unit 1 and the toothed unit 2.

[0045] As an optional implementation manner, the scraping unit 3 includes a main body portion 31. The main body portion 31 is connected to the main body unit 1 and the length of the main body portion 31 is the same as the length of the main body unit 1. The main body portion 31 extends in the width direction of the main body unit 1 relative to the main body unit 1;

[0046] The thickness of the main body portion 31 gradually decreases from the end close to the main body unit 1 to the end far from the main body unit 1, so that its cross-sectional profile forms a trapezoidal effect. With such a setting, it can scrape the PCR plate surface more effectively during use and discharge the air between the sealing plate film and the PCR plate.

[0047] As an optional implementation manner, the scraping unit 3 further includes an extension portion 32. One end of the main body portion 31 close to the toothed unit 2 in the length direction extends outward to form the extension portion 32. The outer side wall of the scribing structure 21 closest to the scraping unit 3 in the toothed unit 2 is connected to the extension portion 32. With such a setting, the effective scraping area of the scraping unit 3 can be expanded, and the overall structural strength can be further improved, making the overall structural form more beautiful.

[0048] As an alternative embodiment, it further includes a support unit 4. The support unit 4 is connected to the main body unit 1, and the support unit 4 can effectively enhance the overall structural strength of the main body unit 1, making it not easily bend and deform.

[0049] As an alternative embodiment, the support unit 4 includes a ribbed support structure 41. Each scoring structure 21 is respectively connected to a ribbed support structure 41 on both the front side and the back side of the main body unit 1;

[0050] The length direction of the ribbed support structure 41 is parallel to the length direction of the main body unit 1. One end of the ribbed support structure 41 is connected to the scoring structure 21. The main plane of the ribbed support structure 41 is connected to the surface of the main body unit 1. The other end of the ribbed support structure 41 extends to be flush with the end of the main body unit 1 away from the scoring structure 21;

[0051] The ribbed support structure 41 has a second ridge 411. The second ridge 411 is collinear with the corresponding first ridge 211. Therefore, the ribbed support structure 41 is equivalent to the continuation of the scoring structure 21 on the main body unit 1. The ribbed support structure 41 is symmetrically formed into a triangular prism along the second ridge 411.

[0052] As an alternative embodiment, it further includes an auxiliary holding unit 5. The auxiliary holding unit 5 is connected to the main body unit 1. The presence of the auxiliary holding unit 5 is more prominent and convenient for the operator to hold the film sealer. At the same time, the auxiliary holding unit 5 can guide the operator, especially for first-time users, and can guide the operator to hold it accurately.

[0053] As an alternative embodiment, the auxiliary holding unit 5 preferably includes a holding platform structure 51. In this embodiment, since the main body unit 1 has a front side and a back side, the number of the holding platform structures 51 is preferably two. The two holding platform structures 51 are respectively connected to the front and back of the main body unit 1. Such a setting can make the structure more symmetrical and more beautiful. More importantly, the symmetrical structure design enables both the operator's left hand and right hand to hold it, which is more convenient;

[0054] Refer to Figure 4 As shown in the figure, when viewed from the thickness direction, the holding platform structure 51 protrudes outward as a whole compared to the front and back of the main body unit 1, so it has a certain thickness. The top surface of the holding platform structure 51 is flush with the ridge edge of the first ridge 211 or the second ridge 411;

[0055] Refer to Figure 3, when viewed from the plane projection direction, the holding platform structure 51 spans and occupies the space between several prismatic support structures 41. In the attached drawings of this embodiment, a structural form spanning and occupying the space between three prismatic support structures 41 is preferably shown. At the same time, the holding platform structure 51 is preferably formed by cutting off small cuboids at both diagonal ends of a large cuboid to form a special-shaped structure. Based on the figure shown, the upper left corner and the lower right corner are cut off, and a smooth transition inclined plane is provided along the length direction of the main body unit 1 between the top surface of the holding platform structure 51 and the surface of the main body unit 1;

[0056] In addition to the holding platform structure 51, the auxiliary holding unit 5 can be flexibly adjusted to other structural forms according to actual usage requirements, so as to further improve the hand feeling, or be matched with other external accessories, such as selecting the structure form of a handle for holding, as an alternative to the holding platform structure 51.

[0057] In this embodiment, during the production process of the handheld multi-tooth PCR plate sealer, it is preferably integrally formed by 3D printing, so it can be easily produced and has better structural strength at the same time;

[0058] The production material of the sealer is preferably white photosensitive resin. White photosensitive resin is suitable for 3D printing production and can save costs. In addition, the material of the sealer can be flexibly changed according to actual usage requirements. For example, in order to achieve different toughness, durability, environmental protection, and appearance characteristics, other materials such as rubber, PP plastic, and PLA plastic can also be used.

[0059] In this embodiment, actually, the main body unit 1, the toothed unit 2, the scraping unit 3, the support unit 4, and the auxiliary holding unit 5 are uniformly symmetrically arranged along the central plane of the main body unit 1. With this arrangement, while the structure is more beautiful, the operator can flexibly choose to hold and use it with the left hand or the right hand, and it is also easier to produce and is conducive to rapid 3D printing.

[0060] In this embodiment, referring to the attached Figure 3 , based on the figure shown, rounded edge decorations are provided at the lower left corner of the main body unit 1, the connection part between the main body unit 1 and the toothed unit 2, and the upper right corner and the lower right corner of the scraping unit 3. The rounded edge decorations can improve the hand feeling of the sealer, prevent sharp tips from damaging the operator's hand, and can also play a role in scribing the sealing edge of the PCR plate.

[0061] In the toothed unit 2 shown in the attached drawings of this embodiment, the distance between the first ridges 211 of adjacent scribing structures 21 is preferably 9 mm, the width of the scribing structure 21 is preferably 1 mm, and the number of scribing structures 21 is five. With this arrangement, it can adapt to the general standard of 96-well PCR plates.

[0062] In this embodiment, the shape and size of the main unit 1 of the film sealer can be slightly changed to better adapt to different plate types of PCR plates or achieve different holding effects;

[0063] Similarly, the width and spacing of the toothed unit 2 of the film sealer can be slightly changed to better adapt to other plate types such as 384-well plates and 48-well plates.

[0064] The above is only the specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention can easily think of changes or substitutions, which should all be covered within the protection scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Claims

1. A hand-held multi-tooth PCR plate sealer, characterized in that, It includes a main body unit (1), a toothed unit (2) and a scraping unit (3). The toothed unit (2) includes a scoring structure (21). A plurality of the scoring structures (21) are connected to the main body unit (1) and are evenly distributed at equal intervals. The scraping unit (3) is connected to the main body unit (1).

2. The hand-held multi-tooth PCR plate sealer according to claim 1, wherein The main body unit (1) is a rectangular plate-like structure. A plurality of the scoring structures (21) are connected to one end of the main body unit (1) in the length direction. The scraping unit (3) is connected to one end of the main body unit (1) in the width direction.

3. The hand-held multi-tooth PCR plate sealer according to claim 1, characterized in that, The scoring structure (21) has two first ridges (211) symmetrically arranged along the thickness direction of the main body unit (1). The scoring structure (21) is symmetrically formed into a prismoid along the left and right sides of the first ridge (211).

4. The hand-held multi-tooth PCR plate sealer according to claim 1, wherein The toothed unit (2) further includes an arc connecting portion (22). One arc connecting portion (22) is connected between two adjacent scoring structures (21). The arc connecting portion (22) is connected to the main body unit (1).

5. The hand-held multi-tooth PCR plate sealer according to claim 1, wherein The scraping unit (3) includes a main body portion (31). The main body portion (31) is connected to the main body unit (1) and the length of the main body portion (31) is the same as the length of the main body unit (1). The thickness of the main body portion (31) gradually decreases from the end close to the main body unit (1) to the end far from the main body unit (1).

6. The hand-held multi-tooth PCR plate sealer according to claim 5, wherein, The scraping unit (3) further includes an extension portion (32). The main body portion (31) extends outward at one end close to the toothed unit (2) in the length direction to form the extension portion (32). The outer side wall of the scoring structure (21) closest to the scraping unit (3) in the toothed unit (2) is connected to the extension portion (32).

7. The hand-held multi-tooth PCR plate sealer according to claim 3, characterized in that, It further includes a support unit (4). The support unit (4) is connected to the main body unit (1).

8. The hand-held multi-tooth PCR plate sealer according to claim 7, characterized in that, The support unit (4) includes a prismatic support structure (41). Each scoring structure (21) is respectively connected to one prismatic support structure (41) on the front side and the back side of the main body unit (1). The prismatic support structure (41) is connected to the main body unit (1). The length direction of the prismatic support structure (41) is parallel to the length direction of the main body unit (1). The prismatic support structure (41) has a second ridge (411). The second ridge (411) is collinear with the corresponding first ridge (211). The prismatic support structure (41) is symmetrically formed into a triangular prism along the second ridge (411).

9. The hand-held multi-tooth PCR plate sealer according to claim 8, wherein It further includes an auxiliary holding unit (5). The auxiliary holding unit (5) is connected to the main body unit (1).

10. The hand-held multi-tooth PCR plate sealer according to claim 9, characterized in that, The auxiliary holding unit (5) includes a holding platform structure (51). Two holding platform structures (51) are respectively connected to the front and the back of the main body unit (1). The top surface of the holding platform structure (51) is flush with the ridge edge of the first ridge (211) or the second ridge (411).

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