Automatic tube and box sealing machine for nucleic acid testing

Abstract

The present invention discloses an automatic tube sealing and box sealing machine for nucleic acid detection, which belongs to the technical field of nucleic acid detection. The automatic tube sealing and box sealing machine for nucleic acid detection includes a transport box, a side of which is provided with a sample placement port and a sampling port in a straight line, and further includes a grabbing assembly, a transport assembly, a rotating assembly, a clamping assembly and a capping assembly placed in the transport box. The capping assembly and the clamping assembly are used in conjunction with each other to unscrew or fasten the tube cap of a reagent tube. The present invention forms a closed loop through the transport device, the grabbing assembly, the clamping assembly and the capping assembly. During the detection process, the reagent tube is taken out - the reagent tube is opened - the reagent tube is closed - the reagent tube is recovered and the sampling robot is cooperated to complete all the work of nucleic acid detection, which can truly achieve unmanned operation during the nucleic acid detection process and realize the automated operation of nucleic acid detection.

Description

Technical Field

[0001] The present invention relates to the technical field of nucleic acid detection, and in particular to an automatic tube and box sealing machine for nucleic acid detection. Background Art

[0002] Currently, the detection of some viral diseases is generally determined by viral nucleic acid testing. During the nucleic acid testing process, reagent tubes, sampling cotton swabs and other tools are generally used to collect samples and transfer them into test tubes.

[0003] For some highly contagious viruses, medical workers collecting the virus need to wear protective clothing. The process of viral nucleic acid testing can be divided into sample collection and sample recovery.

[0004] In the existing technology, the collection process can be replaced by a robotic arm, and most nucleic acid sampling robotic arms can only complete the sampling part with a cotton swab. The robotic arm takes the sample and places the throat swab in the reagent tube after sampling. However, after the sampling is completed, the reagent tube still needs the staff to screw the cap to seal the tube, and then place the sealed tube in a storage box and send it to the testing center. However, in the process of sealing the tube and the box, it is difficult for the staff to avoid contact with the sample to be tested, which will cause a certain risk of infection. Summary of the Invention

[0005] The purpose of the present invention is to overcome the problems in the prior art and provide an automatic tube and box sealing machine for nucleic acid testing, so as to realize unmanned operation during the nucleic acid testing process and realize automated operation of nucleic acid testing.

[0006] The present invention provides an automatic tube sealing machine for nucleic acid testing, comprising a transport box, a sampling port and a sampling port being straightly penetrated on the side surface of the transport box, a storage box for storing reagent tubes being placed in the transport box, and a grabbing assembly being placed in the transport box for grabbing the reagent tubes, the transport assembly being placed in the transport box and arranged above the grabbing assembly for driving the grabbing assembly to move along the direction of the connecting line between the sampling port and the sampling port, the rotating assembly being placed in the transport box and arranged close to the sampling port, the rotating assembly comprising a rotating disk, a plurality of placement slots being opened on the circumference of the rotating disk, the placement slots being used for temporarily placing the reagent tubes grabbed by the grabbing assembly, the clamping assembly being arranged on the inner side of the transport box and above the rotating disk, the clamping assembly comprising a test tube clamp and a horizontally arranged fixing rod, The test tube clamps are provided in a pair, and the pair of test tube clamps are both slidably sleeved on the fixing rod, and the pair of test tube clamps are connected to a first driving member for driving the test tube clamps to move on one side away from each other, and the capping assembly is placed in the transport box, and the capping assembly includes a capping head, and the capping head is located above the clamping assembly, and the top of the capping head is connected to a second driving member. The reagent tube stored on the storage box is grabbed by the grabbing assembly, placed in the placement slot through the transport assembly, and the rotating disk rotates to turn the reagent tube to one side of the clamping assembly, and the capping assembly cooperates with the clamping assembly to unscrew the tube cap of the reagent tube, and the robotic arm places the pharyngeal swab in the reagent tube through the sampling port, and then tightens the reagent tube again through the capping assembly, and puts the reagent tube back into the storage box through the grabbing assembly and the transport assembly.

[0007] Preferably, the transport assembly includes a transport frame, a toothed belt, a first gear, a second gear, a first motor, a guide rail and a column. The guide rails are provided in pair, and the bottoms of both horizontal ends of a pair of guide rails are fixedly connected to the columns, and the bottoms of several columns are fixedly connected to the transport box. The toothed belt is sleeved on the guide rails, and the transport frame is slidingly connected to the guide rails. The first motor is fixedly connected to one side of the guide rails, and the output end is fixedly connected to the first gear. The second gear is rotatably connected to the transport frame, and the first gear and the second gear are both engaged with the toothed belt, and the transport frame moves horizontally on the pair of guide rails.

[0008] Preferably, the grabbing assembly includes a grabbing head, a cylinder and a carrier frame, the carrier frame is horizontally slidably connected to the bottom of the transport frame, the cylinder is vertically arranged and fixedly connected to the carrier frame, and the end of the cylinder is fixedly connected to the grabbing head.

[0009] Preferably, the rotating assembly also includes a fifth motor, a third gear and a fourth gear, the fifth motor is vertically fixedly connected to the transport box, the output end of the fifth motor is fixedly connected to the third gear, the fourth gear is engaged with one side of the third gear, the support rod is vertically arranged, one end is rotatably connected to the transport box, and the other end is fixedly connected to the rotating disk, and the fourth gear is fixedly sleeved on the support rod.

[0010] Preferably, the first driving member also includes a first fixing frame, a double-headed screw and a second motor, the first fixing frame is arranged as a pair, the pair of first fixing frames are horizontally fixedly connected to the side wall of the transport box, the double-headed screw is rotatably connected between the pair of first fixing frames, a pair of threads on the double-headed screw are arranged in opposite directions, the second motor is fixedly connected to one side of the first fixing frame, the output end of the second motor is fixedly connected to the double-headed screw, and a pair of test tube clamps are threadedly sleeved on the double-headed screw.

[0011] Preferably, the second driving member includes a second fixing frame, a third motor, a threaded rod, a nut, a connecting plate and a fourth motor, the second fixing frame is fixedly connected to the transport box, the third motor is vertically fixedly connected to the bottom of the second fixing frame, the output end of the third motor is fixedly connected to the threaded rod, the nut is threadedly sleeved on the threaded rod, the connecting plate is horizontally fixedly connected to one side of the nut, the fourth motor is vertically fixedly connected to the connecting plate, the output end of the fourth motor is fixedly connected to the capping head, the nut is horizontally fixedly connected to a sliding rod on the side away from the connecting plate, and the sliding rod is vertically slidably connected to the inner wall of the transport box at one end away from the connecting plate.

[0012] Preferably, a stabilizing plate is fixedly connected to the top of the support rod, and a plurality of stabilizing grooves are provided on the side of the stabilizing plate. The plurality of stabilizing grooves and the plurality of placement grooves are arranged in a one-to-one correspondence and their axes coincide with each other.

[0013] Preferably, the grabbing head is configured to be medical clean rubber, and the inner diameter of the grabbing head is the same as the outer diameter of the anti-slip rough line on the outer side of the reagent tube cover.

[0014] Preferably, the capping head is made of rubber and has an interference fit with the cap of the reagent tube.

[0015] Preferably, support bases are fixedly connected to the bottom corners of the transport box.

[0016] Compared with the prior art, the beneficial effects of the present invention are as follows: an automatic tube sealing and box sealing machine for nucleic acid testing of the present invention is used in conjunction with a sampling robot, and the provided grabbing assembly is moved to the top of the reagent tube in the storage box through the transport assembly, and the reagent tube is grabbed by the grabbing assembly. At this time, the first motor reverses and the transport frame moves toward one side of the clamping assembly under the cooperation of the first gear, the second gear and the toothed belt. When the grabbing assembly moves the reagent tube to one side of the clamping assembly, the reagent tube is clamped and stabilized by the clamping assembly. At this time, the tube cap of the reagent tube is unscrewed by the provided ground screwing assembly, and the sampling robot puts the taken test sample into the reagent tube, and then tightens the tube cap of the reagent tube through the screwing assembly. At this time, the reagent tube is grabbed by the grabbing assembly, and the reagent tube containing the test sample is transported back to the storage box in cooperation with the transport assembly. The sampling process of the present invention forms a closed loop, and the various components cooperate with each other to achieve unmanned participation in the entire process, which saves labor and avoids the risk of infection. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the internal structure of the transport box of the present invention.

[0018] Figure 2 It is a structural schematic diagram of the rotating assembly, the clamping assembly and the cover screwing assembly of the present invention when they cooperate.

[0019] Figure 3 It is a schematic structural diagram of the clamping assembly of the present invention.

[0020] Figure 4 It is a schematic diagram of the overall structure of the present invention.

[0021] Explanation of the accompanying symbols: 1. Transport box; 2. Storage box; 3. Reagent tube; 4. Stabilizing tank; 5. Transport assembly; 51. Transport rack; 52. Toothed belt; 53. First gear; 54. Second gear; 55. First motor; 56. Guide rail; 57. Column; 6. Grabbing assembly; 61. Carrying frame; 62. Cylinder; 63. Grabbing head; 7. Clamping assembly; 71. First fixing frame; 72. Double-headed screw; 73. Second motor ;74. Test tube clamp;75. Fixing rod;8. Capping assembly;81. Second fixing frame;82. Third motor;83. Threaded rod;84. Nut;85. Connecting plate;86. Fourth motor;87. Capping head;9. Rotating assembly;91. Fifth motor;92. Third gear;93. Fourth gear;94. Support rod;95. Rotating disk;96. Placement slot;10. Support seat;11. Lofting port;12. Stabilizing disk. DETAILED DESCRIPTION

[0022] The following is combined with Figures 1 to 4In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings of the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the described embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention. Unless otherwise defined, the technical terms or scientific terms used herein should be the common meanings understood by people with ordinary skills in the field to which the present invention belongs.

[0023] The words "first", "second" and similar words used in the patent application specification and claims of the present invention do not indicate any order, quantity or importance, but are only used to distinguish different components. Words such as "include" or "comprise" mean that the elements or objects appearing before the word include the elements or objects listed after the word and their equivalents, but do not exclude other elements or objects. "Inside", "outside", "upper", "lower", "far", "near", "front", "back" and the like are only used to indicate relative positional relationships. When the absolute position of the described object changes, the relative positional relationship may also change accordingly. The drawings in the present invention are not drawn strictly according to the actual scale. The specific size and quantity of each structure can be determined according to actual needs. The drawings described in the present invention are only structural schematic diagrams.

[0024] The present invention provides an automatic tube sealing machine for nucleic acid detection, such as Figures 1 to 4 As shown, it includes a transport box 1, a sample opening 11 and a sampling port are opened on the side of the transport box 1 in a straight line, a storage box 2 for storing the reagent tube 3 is placed in the transport box 1, and a grabbing assembly 6 is placed in the transport box 1 for grabbing the reagent tube 3. The transport assembly 5 is placed in the transport box 1 and is arranged above the grabbing assembly 6 to drive the grabbing assembly 6 to move along the direction of the connecting line between the sample opening 11 and the sampling port. The rotating assembly 9 is placed in the transport box 1 and is arranged near the sample opening 11. The rotating assembly 9 includes a rotating disk 95. A plurality of placement slots 96 are opened on the circumference of the rotating disk 95. The placement slots 96 are used to grab the grabbing assembly 6 The grabbed reagent tube 3 is temporarily placed, and the clamping assembly 7 is arranged on the inner side of the transport box 1 above the rotating disk 95. The clamping assembly 7 includes a test tube clamp 74 and a fixing rod 75. A pair of test tube clamps 74 are provided, and the pair of test tube clamps 74 are slidably sleeved on the fixing rod 75. The pair of test tube clamps 74 are connected to a first driving member for driving the test tube clamp 74 to move on one side away from each other. The capping assembly 8 is placed in the transport box 1. The capping assembly 8 includes a capping head 87. The capping head 87 is located above the clamping assembly 7. The top of the capping head 87 is connected to a second driving member. The capping assembly 8 is used in conjunction with the clamping assembly 7 to unscrew or buckle the tube cap of the reagent tube 3;

[0025] The reagent tube 3 on the storage and receiving box 2 is grabbed by the grabbing component 6, and placed in the placement slot 96 through the transport component 5. The rotating disk 95 rotates to turn the reagent tube 3 to the side of the clamping component 7. The capping component 8 cooperates with the clamping component 7 to unscrew the tube cap of the reagent tube 3. The robotic arm places the throat swab in the reagent tube 3 through the sampling port 11, and then tightens the reagent tube 3 again through the capping component 8, and puts the reagent tube 3 back into the storage box 2 through the grabbing component 6 and the transport component 5.

[0026] The first gear 53 and the second gear 54 are used to move the transport frame 51 to the side of the clamping assembly 7. When the first gear 53 and the second gear 54 are used to move the transport frame 51 to the side of the clamping assembly 7, the first gear 53 and the second gear 54 are used to move the transport frame 51 to the side of the clamping assembly 7. When the first gear 53 and the second gear 54 are used to move the transport frame 51 to the side of the clamping assembly 7, the first gear 53 and the second gear 54 are used to move the transport frame 51 to the side of the clamping assembly 7. When the first gear 53 and the second gear 54 are used to move the transport frame 51 to the side of the clamping assembly 7, the first gear 53 and the second gear 54 are used to move the transport frame 51 to the side of the clamping assembly 7. When the first gear 53 and the second gear 54 are used to move the transport frame 51 to the side of the clamping assembly 7, the first gear 53 and the second gear 54 are used to move the transport frame 51 to the side of the clamping assembly 7.

[0027] Preferably, Figures 1 to 3 As shown, the transport assembly 5 includes a transport frame 51, a toothed belt 52, a first gear 53, a second gear 54, a first motor 55, a guide rail 56 and a column 57. The bottoms of both ends of a pair of guide rails 56 are fixedly connected to the columns 57, and the bottoms of several columns 57 are fixedly connected to the transport box 1. The toothed belt 52 is sleeved on the guide rail 56, and the transport frame 51 is slidingly connected to the guide rail 56. The first motor 55 is fixedly connected to one side of the guide rail 56, and the output end is fixedly connected to the first gear 53. The second gear 54 is rotatably connected to the transport frame 51. The first gear 53 and the second gear 54 are both engaged with the toothed belt 52. The transport frame 51 moves horizontally on the pair of guide rails 56, driving the reagent tube 3 to move horizontally.

[0028] The first motor 55 drives the first gear 53 to rotate, and the first gear 53 engages with the toothed belt 52. Under the action of the second gear 54, the transport frame 51 moves horizontally on a pair of guide rails 56, thereby driving the grabbing assembly 6 to move.

[0029] Preferably, Figures 1 and 2As shown, the grabbing assembly 6 includes a grabbing head 63, a cylinder 62 and a carrier frame 61. The carrier frame 61 is slidably connected to the bottom of the transport frame 51. The cylinder 62 is vertically arranged and fixedly connected to the carrier frame 61. The end of the cylinder 62 is fixedly connected to the grabbing head 63. The grabbing head 63 is set to medical clean rubber. The inner diameter of the grabbing head 63 is the same as the outer diameter of the anti-slip rough line on the outside of the tube cover of the reagent tube 3. The screwing head 87 is made of rubber and has an interference fit with the tube cap of the reagent tube 3. The support base 10 is fixedly connected to the bottom corners of the transport box 1.

[0030] In this embodiment, the carrier 61 is slidably arranged on a pair of guide rails 56. When the carrier 61 moves to the top of the reagent tube 3, the grabbing head 63 is pushed downward by the cylinder 62 to grab the reagent tube 3 placed in the storage box 2. In the present invention, the grabbing head 63 is set to medical clean rubber, and the inner diameter of the grabbing head 63 is designed to be the same as the outer diameter of the anti-slip rough line on the outside of the tube cover of the reagent tube 3, ensuring that the sample in the reagent tube 3 will not be contaminated after the sampling is completed, ensuring that the reagent tube 3 will not fall and avoiding damage to the reagent tube 3 due to excessive force. The grabbing head 63 can open and close automatically.

[0031] Preferably, Figures 1 and 2 As shown, the rotating assembly 9 also includes a fifth motor 91, a third gear 92 and a fourth gear 93. The fifth motor 91 is vertically fixedly connected to the transport box 1. The output end of the fifth motor 91 is fixedly connected to the third gear 92. The fourth gear 93 is engaged with one side of the third gear 92. The support rod 94 is vertically arranged, one end is rotatably connected to the transport box 1, and the other end is fixedly connected to the rotating disk 95. The fourth gear 93 is fixedly sleeved on the support rod 94.

[0032] In this embodiment, the transport box 1 supports the fifth motor 91, and the fifth motor 91 drives the third gear 92 to rotate. The third gear 92 engages with the fourth gear 93, thereby driving the support rod 94 to rotate, and the support rod 94 drives the rotating disk 95 to rotate. The transport component 5 places the transported reagent tube 3 in the placement slot 96 opened on the rotating disk 95, standardizes the verticality of the reagent tube 3, and can complete the work of the reagent tube 3 in the working box, ensuring that the subsequent work such as screwing the cap and collecting the throat swab can be carried out smoothly.

[0033] Preferably, Figures 1 to 3 As shown, the first driving member includes a first fixing frame 71, a double-headed screw 72 and a second motor 73. The first fixing frame 71 is arranged as a pair. The pair of first fixing frames 71 are horizontally fixedly connected to the side wall of the transport box 1. The double-headed screw 72 is rotatably connected between the pair of first fixing frames 71. A pair of threads on the double-headed screw 72 are set in opposite directions. The second motor 73 is fixedly connected to one side of the first fixing frame 71. The output end of the second motor 73 is fixedly connected to the double-headed screw 72. A pair of test tube clamps 74 are threadedly sleeved on the double-headed screw 72.

[0034] In this embodiment, the transport box 1 supports the clamping assembly 7. When the reagent tube 3 is transported to one side of the clamping assembly 7 by the transport assembly 5, the double-headed screw 72 is driven to rotate by the second motor 73, so that the pair of test tube clamps 74 are moved under the guidance of the fixed rod 75 to stably clamp the reagent tube 3, and are used together with the cap screwing assembly 8 to unscrew the tube cap of the reagent tube 3, so that the robotic arm can place the cotton swab with completed sampling in the opened reagent tube 3.

[0035] Preferably, Figures 1 to 3 As shown, the second driving member includes a second fixing frame 81, a third motor 82, a threaded rod 83, a nut 84, a connecting plate 85 and a fourth motor 86. The second fixing frame 81 is fixedly connected to the transport box 1, the third motor 82 is vertically fixedly connected to the bottom of the second fixing frame 81, the output end of the third motor 82 is fixedly connected to the threaded rod 83, the nut 84 is threadedly sleeved on the threaded rod 83, the connecting plate 85 is horizontally fixedly connected to one side of the nut 84, the fourth motor 86 is vertically fixedly connected to the connecting plate 85, the output end of the fourth motor 86 is fixedly connected to the capping head 87, and the nut 84 is horizontally fixedly connected to a sliding rod on the side away from the connecting plate 85, and the sliding rod is vertically slidably connected to the inner wall of the transport box 1 at one end away from the connecting plate 85.

[0036] In this embodiment, the transport box 1 supports the second fixing frame 81, and the second fixing frame 81 supports the third motor 82. After the clamping assembly 7 clamps the reagent tube 3, the third motor 82 rotates to make the nut 84 move down steadily on the threaded rod 83 with the assistance of the sliding rod. Here, the threaded rod 83 and the nut 84 are used to cooperate with the capping head 87 to perform precise vertical regulation. When the capping head 87 covers the reagent tube 3, the fourth motor 86 drives the capping head 87 to rotate, unscrews the tube cap of the reagent tube 3, and then moves the capping head 87 up by the third motor 82. The capping head 87 achieves the fixation and rotation of the tube cap of the reagent tube 3 through interference fit.

[0037] Preferably, Figures 1 to 3 As shown, a stabilizing plate 12 is fixedly connected to the top of the support rod 94 , and a plurality of stabilizing grooves 4 are opened on the side of the stabilizing plate 12 . The plurality of stabilizing grooves 4 are arranged in a one-to-one correspondence with the plurality of placement grooves 96 and their axes coincide with each other.

[0038] The stability of the placed reagent tube 3 is ensured by the stabilizing plate 12 , and because the stabilizing groove 4 and the placement groove 96 are arranged in a one-to-one correspondence and their axes coincide, the reagent tube 3 is ensured to be placed vertically, thereby preventing the reagent tube 3 from tipping over.

[0039] The method of using the automatic tube sealing and box sealing machine for nucleic acid detection of the present invention is as follows:

[0040] The first motor 55 drives the first gear 53 to rotate, and the first gear 53 is engaged with the toothed belt 52. Under the action of the second gear 54, the transport frame 51 moves horizontally on the pair of guide rails 56, and moves the grabbing assembly 6 to the top of the reagent tube 3 in the storage box 2.

[0041] The grabbing head 63 is pushed downward by the cylinder 62 to grab the reagent tube 3 placed in the storage box 2. In the present invention, the grabbing head 63 is made of medical clean rubber, and the inner diameter of the grabbing head 63 is the same as the outer diameter of the anti-slip rough line on the outer side of the tube cover of the reagent tube 3, which ensures that the sample in the reagent tube 3 will not be contaminated after the sampling is completed. It not only ensures that the reagent tube 3 does not fall but also prevents the reagent tube 3 from being damaged due to excessive force. After the reagent tube 3 is grabbed, the first motor 55 is reversed, and the transport frame 51 moves toward the side of the clamping assembly 7 under the cooperation of the first gear 53, the second gear 54 and the toothed belt 52;

[0042] The grabbed reagent tube 3 is placed in the placement slot 96 in the rotating disk 95. The second motor 73 drives the double-headed screw 72 to rotate, so that the pair of test tube clamps 74 are moved under the guidance of the fixed rod 75 to stably clamp the reagent tube 3.

[0043] After the clamping assembly 7 clamps the reagent tube 3, the third motor 82 rotates to cause the nut 84 to move down steadily on the threaded rod 83 with the assistance of the sliding rod. Here, the threaded rod 83 and the nut 84 cooperate to precisely control the capping head 87 in the vertical direction. When the capping head 87 covers the reagent tube 3, the fourth motor 86 drives the capping head 87 to rotate to unscrew the cap of the reagent tube 3. The robotic arm places the sample collected into the reagent tube 3 through the sampling port 11, thereby completing the sampling. The reagent tube 3 is grabbed by the grabbing head 63 and placed in the placement slot 96 through the transport assembly 5. The rotating disk 95 rotates to turn the reagent tube 3 to the side of the clamping assembly 7. The capping assembly 8 cooperates with the clamping assembly 7 to unscrew the cap of the reagent tube 3. The robotic arm places the throat swab into the reagent tube 3 through the sampling port 11, and then tightens the reagent tube 3 again through the capping assembly 8. The grabbing assembly 6 and the transport assembly 5 cooperate to place the reagent tube 3 in the storage box 2.

[0044] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. An automatic tube and carton sealing machine for nucleic acid testing, characterized in that: The transport box comprises a transport box, a sample placement port and a sampling port are provided on the side of the transport box in a straight line, a storage box for storing reagent tubes is placed in the transport box, and the following is placed in the transport box: A grabbing component, used for grabbing the reagent tube; A transport assembly is provided above the grabbing assembly and is used to drive the grabbing assembly to move along the direction of the connecting line between the lofting port and the sampling port; A rotating assembly is arranged near the sample opening, the rotating assembly includes a rotating disk, and a plurality of placement grooves are opened on the rotating disk in a circumferential direction, and the placement grooves are used to temporarily place the reagent tubes grabbed by the grabbing assembly; A clamping assembly is located above the rotating disk, the clamping assembly includes a test tube clamp and a horizontally arranged fixed rod, the test tube clamp is provided in a pair, the test tube clamps are both slidably sleeved on the fixed rod, and the test tube clamps are both connected to a first driving member for driving the test tube clamps to move on one side away from each other; A capping assembly, comprising a capping head, the capping head being located above the clamping assembly, the top of the capping head being connected to a second driving member; The reagent tube stored in the storage box is grabbed by the grabbing assembly and placed in the placement slot through the transport assembly. The rotating disk rotates to turn the reagent tube to the side of the clamping assembly. The capping assembly cooperates with the clamping assembly to unscrew the tube cap of the reagent tube. The robotic arm places the throat swab in the reagent tube through the sampling port, and then tightens the reagent tube again through the capping assembly, and puts the reagent tube back into the storage box through the grabbing assembly and the transport assembly.

2. The automatic tube and box sealing machine for nucleic acid detection according to claim 1, characterized in that: The transport assembly includes a transport frame, a toothed belt, a first gear, a second gear, a first motor, a guide rail and a column. The guide rails are provided in pair, and the bottoms of both horizontal ends of the pair of guide rails are fixedly connected to the columns. The bottoms of several columns are fixedly connected to the transport box. The toothed belt is sleeved on the guide rails, and the transport frame is slidingly connected to the guide rails. The first motor is fixedly connected to one side of the guide rails, and the output end is fixedly connected to the first gear. The second gear is rotatably connected to the transport frame. The first gear and the second gear are both engaged with the toothed belt, and the transport frame moves horizontally on the pair of guide rails.

3. The automatic tube and box sealing machine for nucleic acid detection according to claim 2, characterized in that: The grabbing assembly includes a grabbing head, a cylinder and a carrier frame. The carrier frame is horizontally slidably connected to the bottom of the transport frame. The cylinder is vertically arranged and fixedly connected to the carrier frame. The end of the cylinder is fixedly connected to the grabbing head.

4. The automatic tube and box sealing machine for nucleic acid detection according to claim 1, characterized in that: The rotating assembly also includes a fifth motor, a third gear and a fourth gear. The fifth motor is vertically fixedly connected to the transport box, the output end of the fifth motor is fixedly connected to the third gear, and the fourth gear is engaged with one side of the third gear. The support rod is vertically arranged, one end is rotatably connected to the transport box, and the other end is fixedly connected to the rotating disk. The fourth gear is fixedly sleeved on the support rod.

5. The automatic tube and box sealing machine for nucleic acid detection according to claim 1, characterized in that: The first driving member includes a first fixing frame, a double-headed screw and a second motor. The first fixing frames are arranged as a pair. The pair of first fixing frames are horizontally fixedly connected to the side walls of the transport box. The double-headed screw is rotatably connected between the pair of first fixing frames. A pair of threads on the double-headed screw are arranged in opposite directions. The second motor is fixedly connected to one side of the first fixing frame. The output end of the second motor is fixedly connected to the double-headed screw. A pair of test tube clamps are threadedly sleeved on the double-headed screw.

6. The automatic tube and box sealing machine for nucleic acid detection according to claim 1, characterized in that: The second driving member includes a second fixing frame, a third motor, a threaded rod, a nut, a connecting plate and a fourth motor. The second fixing frame is fixedly connected to the transport box, the third motor is vertically fixedly connected to the bottom of the second fixing frame, the output end of the third motor is fixedly connected to the threaded rod, the nut is threadedly sleeved on the threaded rod, the connecting plate is horizontally fixedly connected to one side of the nut, the fourth motor is vertically fixedly connected to the connecting plate, the output end of the fourth motor is fixedly connected to the capping head, the nut is horizontally fixedly connected to a sliding rod on the side away from the connecting plate, and the sliding rod is vertically slidably connected to the inner side wall of the transport box at one end away from the connecting plate.

7. The automatic tube and box sealing machine for nucleic acid detection according to claim 4, characterized in that: The top of the support rod is fixedly connected with a stabilizing plate, and a plurality of stabilizing grooves are opened on the side of the stabilizing plate. The plurality of stabilizing grooves are arranged in a one-to-one correspondence with the plurality of placement grooves, and their axes coincide with each other.

8. The automatic tube and box sealing machine for nucleic acid detection according to claim 3, characterized in that: The grabbing head is configured to be medical clean rubber, and the inner diameter of the grabbing head is the same as the outer diameter of the anti-slip rough line on the outer side of the reagent tube cover.

9. The automatic tube and box sealing machine for nucleic acid detection according to claim 1, characterized in that: The capping head is made of rubber and has an interference fit with the cap of the reagent tube.

10. The automatic tube and box sealing machine for nucleic acid detection according to claim 1, characterized in that: The bottom corners of the transport box are all fixedly connected with support seats.

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