Escherichia coli sampling and detecting equipment for mouse experiment

Abstract

The invention relates to the field of microorganism sampling detection, in particular to escherichia coli sampling detection equipment for mouse experiments. The technical problem to be solved is that when mouse intestinal escherichia coli is sampled and detected, attention needs to be highly concentrated during manual operation, physical and mental exhaustion is easily caused, and the intestinal tract is damaged by slight carelessness. In addition, in the prior art, adipose tissue existing on the outer surface of the intestinal tract cannot be removed, and therefore the sampling detection result is affected. According to the technical scheme, the escherichia coli sampling and detecting equipment for the mouse experiment comprises a sampling box, a fixing assembly and the like; and a fixing assembly is mounted in the sampling box. According to the device, the left end and the right end of the intestinal tract of a mouse are fixed through the first clamping block and the second clamping block respectively, sterile water is injected into the intestinal tract of the mouse through an injection pipe for flushing, and cleaning of the intestinal tract of the mouse is completed, so that the pressure of manual operation is relieved, and meanwhile accidental damage to the intestinal tract of the mouse caused by manual operation is reduced.

Description

Technical Field

[0001] The present invention relates to the field of microbial sampling and detection, and particularly to an Escherichia coli sampling and detection device for mouse experiments. Background Art

[0002] When sampling and detecting Escherichia coli in the mouse intestine, first, the entire mouse intestine is taken out under aseptic conditions, and then the intestine is washed with sterile water. However, due to the small size of the mouse intestine, high concentration of attention is required during manual operation, which is extremely likely to cause physical and mental fatigue. Even a slight carelessness may cause damage to the intestine and affect the sampling and detection results.

[0003] At the same time, adipose tissue usually exists on the outer surface of the intestine. When sampling and detecting the intestine, the adipose tissue that has not been removed will affect the accuracy of the sampling results. The existing technology cannot remove the adipose tissue existing on the outer surface of the intestine, resulting in inaccurate sampling and detection results. Summary of the Invention

[0004] In order to overcome the disadvantages that during the sampling and detection of Escherichia coli in the mouse intestine, high concentration of attention is required during manual operation, which is extremely likely to cause physical and mental fatigue, and even a slight carelessness may cause damage to the intestine, and at the same time, the existing technology cannot remove the adipose tissue existing on the outer surface of the intestine, thus affecting the sampling and detection results, the present invention provides an Escherichia coli sampling and detection device for mouse experiments.

[0005] The technical solution is as follows: An Escherichia coli sampling and detection device for mouse experiments includes a sampling box; it further includes a fixing component, a placement block, a first clamping block, a second clamping block, an injection tube, and a scraping system; the fixing component is installed inside the sampling box; a placement block for placing the mouse intestine is arranged inside the sampling box; a placement groove is arranged on the placement block; a first clamping block for fixing the left end of the mouse intestine is slidably connected to the left side of the sampling box; a second clamping block for fixing the right end of the mouse intestine is slidably connected to the right side of the sampling box; an injection tube for flushing the inside of the mouse intestine is installed on the left side of the sampling box, and the injection tube is arranged as a conical tube horizontally placed to the right; a scraping system for scraping the adipose tissue on the outer surface of the mouse intestine is arranged on the placement block.

[0006] Preferably, the fixing component includes a fixing plate, a first fixing rod, and a second fixing rod; a fixing plate is fixedly connected to the middle of the sampling box, and the lower side of the placement block is fixedly connected to the fixing plate; a first fixing rod is slidably connected to the left side of the sampling box, and the first clamping block is fixedly connected to the first fixing rod; a second fixing rod is slidably connected to the right side of the sampling box, and the second clamping block is fixedly connected to the second fixing rod.

[0007] Preferably, it further includes a pressing rod; a pressing rod for pressing the contents inside the mouse intestine is slidably connected to the left side of the sampling box, and the pressing rod is located inside the injection tube.

[0008] Preferably, it further includes a fixed disk, an arc-shaped rod, and a limit ring; the right side of the extrusion rod is slidably connected to the fixed disk; several round rods for restricting the movement of the fixed disk are fixedly connected to the fixed disk; several arc-shaped rods are fixedly connected to the fixed disk, and each arc-shaped rod is made of a deformable material; several arc-shaped rods are all located inside the injection tube; the right side of the extrusion rod is fixedly connected to the limit ring, and the limit ring is located to the right of the fixed disk.

[0009] Preferably, the scraping system includes a connecting block and arc-shaped pieces; several sliding grooves are arranged on the placing block, and the placing groove is located between several sliding grooves; a connecting block is slidably connected together between several sliding grooves; several arc-shaped pieces are movably connected to the connecting block, and several arc-shaped pieces are rotationally connected to each other; each arc-shaped piece is arranged as a downwardly convex arc shape, and the lower side of the lower arc-shaped piece is attached to the surface of the placing groove; several scraping pieces for scraping the adipose tissue on the outer surface of the mouse intestine are arranged on each arc-shaped piece.

[0010] Preferably, it further includes a baffle; a baffle for preventing adipose tissue from remaining on the placing groove is fixedly connected to the concave part of the upper arc-shaped piece.

[0011] Preferably, it further includes a semi-ring and a pushing block; an arc-shaped block is arranged on the upper side of the second clamping block; a rotating block is arranged on the lower side of the second clamping block; a semi-ring is fixedly connected to each of the arc-shaped block and the rotating block, and the two semi-rings are attached together to form a complete circular ring shape; grooves are arranged on both the upper and lower sides of the second clamping block, and the semi-ring slides in the groove; a pushing block for improving the cleaning effect on the outer surface of the mouse intestine is fixedly connected to the right side of the rotating block of the second clamping block.

[0012] Preferably, it further includes a curved arc piece and a fixing block; several curved arc pieces for extruding the contents of the mouse intestine are fixedly connected to the right side of the extrusion rod, and each curved arc piece is made of a deformable material; a fixing block is fixedly connected together on the right side of several curved arc pieces.

[0013] Preferably, it further includes a ball; several balls are rotatably connected to the middle of each curved arc piece.

[0014] Preferably, it further includes a rubber sheet; a rubber sheet is fixedly connected between every two adjacent curved arc pieces.

[0015] Advantages of the present invention: The present invention realizes fixing the left end and the right end of the mouse intestine by the first clamping block and the second clamping block respectively, so that the injection tube injects sterile water into the mouse intestine for flushing, thereby completing the cleaning of the mouse intestine, reducing the pressure of manual operation and reducing accidental damage to the mouse intestine caused by manual operation; scraping the adipose tissue on the outer surface of the mouse intestine by the scraping pieces on the two arc-shaped pieces pressed together, thereby preventing the existence of adipose tissue from affecting the accuracy of the sampling and detection results; The fat tissue after scraping is moved to the right along with the arc-shaped piece through the baffle, preventing the fat tissue after scraping from staying in the placement groove, avoiding affecting the observation of the outer surface of the mouse intestine by the tester, and improving the cleaning effect of the outer surface of the mouse intestine; The right end of the mouse intestine is driven to rotate by the pushing block, so that the scraping piece on the arc-shaped piece scrapes the fat tissue on various parts of the outer surface of the mouse intestine, thereby further improving the cleaning effect of the outer surface of the mouse intestine; The contact area with the mouse intestinal contents is increased through the curved arc piece, thereby improving the discharge effect of the contents in the mouse intestine. At the same time, the inner wall of the mouse intestine is contacted by the ball and the adhered contents on the inner wall are scraped, thereby further improving the cleaning effect of the inner wall of the mouse intestine and avoiding incomplete cleaning, which affects the subsequent sampling and detection results. Description of the Drawings

[0016] Figure 1 It is a three-dimensional structural schematic diagram of the Escherichia coli sampling and detection device for mouse experiments of the present invention; Figure 2 It is a cross-sectional view of the sampling box of the present invention; Figure 3 It is a cross-sectional view of the injection tube of the present invention; Figure 4 It is a three-dimensional structural schematic diagram of the combination of the fixed disk, arc-shaped rod and limit ring of the present invention; Figure 5 It is a first three-dimensional structural schematic diagram of the scraping system of the present invention; Figure 6 It is a second three-dimensional structural schematic diagram of the scraping system of the present invention; Figure 7 It is a three-dimensional structural schematic diagram of the combination of the semi-ring and the pushing block of the present invention; Figure 8 It is a three-dimensional structural schematic diagram of the combination of the curved arc piece and the ball of the present invention; Figure 9 It is a three-dimensional structural schematic diagram of the combination of the curved arc piece, the ball and the rubber piece of the present invention.

[0017] Description of the Reference Numerals: 1 - sampling box, 2 - placement block, 2001 - placement groove, 2002 - sliding groove, 3 - first clamping block, 3001 - groove, 4 - second clamping block, 4001 - arc-shaped block, 4002 - rotating block, 5 - injection tube, 101 - fixing plate, 102 - first fixing rod, 103 - second fixing rod, 104 - extrusion rod, 105 - fixed disk, 106 - arc-shaped rod, 107 - limit ring, 201 - connecting block, 202 - arc-shaped piece, 20201 - scraping piece, 203 - baffle, 301 - semi-ring, 302 - pushing block, 401 - curved arc piece, 402 - ball, 403 - rubber piece, 404 - fixing block. Detailed Embodiments

[0018] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0019] Embodiment 1 As Figures 2 - 4 shown, an Escherichia coli sampling and detection device for mouse experiments includes a sampling box 1; It further includes a fixing component, a placement block 2, a first clamping block 3, a second clamping block 4, an injection tube 5 and a scraping system; a fixing component is installed in the sampling box 1; a placement block 2 is arranged in the sampling box 1; a placement groove 2001 is arranged on the placement block 2; a first clamping block 3 is slidably connected to the left side of the sampling box 1; a second clamping block 4 is slidably connected to the right side of the sampling box 1; an injection tube 5 is installed on the left side of the sampling box 1, and the injection tube 5 is a conical tube horizontally placed to the right; a scraping system is arranged on the placement block 2; the left end and the right end of the mouse intestine are respectively fixed by the first clamping block 3 and the second clamping block 4, so that the injection tube 5 injects sterile water into the mouse intestine for flushing, thereby completing the cleaning of the mouse intestine, reducing the pressure of manual operation, and at the same time reducing accidental damage to the mouse intestine caused by manual operation.

[0020] The fixing component includes a fixing plate 101, a first fixing rod 102 and a second fixing rod 103; a fixing plate 101 is fixedly connected to the middle of the sampling box 1, and the lower side of the placement block 2 is fixedly connected to the fixing plate 101; a first fixing rod 102 is slidably connected to the left side of the sampling box 1, and the first clamping block 3 is fixedly connected to the first fixing rod 102; a second fixing rod 103 is slidably connected to the right side of the sampling box 1, and the second clamping block 4 is fixedly connected to the second fixing rod 103; the first clamping block 3 and the second clamping block 4 are respectively controlled to move left and right by the first fixing rod 102 and the second fixing rod 103, so as to adjust the position according to different intestinal lengths, facilitating the subsequent work.

[0021] It further includes a pressing rod 104; a pressing rod 104 is slidably connected to the left side of the sampling box 1, and the pressing rod 104 is located inside the injection tube 5; the contents in the mouse intestine are pushed and penetrated by the pressing rod 104, thereby improving the flushing effect of the injection tube 5 on the inside of the mouse intestine.

[0022] It further includes a fixing disk 105, an arc rod 106 and a limiting ring 107; a fixing disk 105 is slidably connected to the right side of the pressing rod 104; four round rods are fixedly connected to the fixing disk 105; four arc rods 106 are fixedly connected to the fixing disk 105, and each arc rod 106 is made of a deformable material; the four arc rods 106 are all located inside the injection tube 5; a limiting ring 107 is fixedly connected to the right side of the pressing rod 104, and the limiting ring 107 is located to the right of the fixing disk 105.

[0023] The following is a detailed description of the mouse intestinal cleaning process: first, the entire mouse intestinal tract after dissection is manually cut into segments according to needs, and then a segment of the mouse intestinal tract is horizontally placed in the placement groove 2001 of the placement block 2, and according to the length of the mouse intestinal tract, the first fixing rod 102 and the second fixing rod 103 are pushed respectively, so that the left end and the right end of the mouse intestinal tract are respectively located on the first clamping block 3 and the second clamping block 4, at this time, the first clamping block 3 and the second clamping block 4 are both in an open state. After the mouse intestinal tract is placed, the first clamping block 3 and the second clamping block 4 are closed, so that the first clamping block 3 and the second clamping block 4 fix the left end and the right end of the mouse intestinal tract respectively, and then according to the length and size of the mouse intestinal tract, a suitable injection is selected. Tube 5, and align the injection tube 5 with the opening at the left end of the mouse intestine. At this time, the tester slowly pushes the squeezing rod 104. At this time, the fixed disk 105 and the curved rod 106 follow the squeezing rod 104 to move to the right until the fixed disk 105 moves to the tube mouth of the injection tube 5. The round rod on the fixed disk 105 is squeezed by the tube mouth of the injection tube 5 and cannot continue to move to the right. At the same time, the right end of the squeezing rod 104 and the curved rod 106 on the fixed disk 105 gradually move from the tube mouth of the injection tube 5 to the inside of the left end of the mouse intestine. With the movement of the curved rod 106, the curved rod 106 gradually breaks away from the squeezing of the injection tube 5 and gradually expands outward, thereby expanding the left end of the mouse intestine. At this time, the injection tube 5 is used to inject the mouse with Sterile water is introduced into the intestine, and part of the left end of the mouse intestine is clamped on the groove 3001 of the first clamping block 3 through the right end of the arc rod 106, thereby increasing the content of sterile water injected into the mouse intestine by the injection tube 5, avoiding that most of the sterile water cannot flow smoothly from the left end of the mouse intestine to the right end of the mouse intestine due to the small opening, reducing resource waste, and preventing the left end of the mouse intestine from being separated from the first clamping block 3 due to the sterile water injection force of the injection tube 5 when the injection tube 5 is flushing the mouse intestine, thereby affecting the cleaning efficiency of the mouse intestine. At the same time, the tester continues to slowly push the squeezing rod 104 to the right according to the state of the mouse intestine, so that the squeezing rod 104 can The contents are squeezed and penetrated, thereby improving the flushing effect of the injection tube 5 on the inside of the mouse intestine until it is flushed clean. After the flushing is completed, the left and right ends of the cleaned mouse intestine are removed as needed, and then the mouse intestine is cut into tissue blocks of appropriate sizes and placed in a sterile freezing tube. The inspector then pulls the squeezing rod 104 to return to its original position. At this time, the limiting ring 107 gradually approaches the expanded arc rod 106 and squeezes the arc rod 106 to move the arc rod 106 to the left. At this time, through the squeezing of the injection tube 5 and the limiting ring 107, the arc rod 106 gradually changes from an expanded state to a contracted state, and gradually enters the interior of the injection tube 5, thereby facilitating the subsequent reuse of the device.

[0024] Example 2 On the basis of Example 1, Figure 5 and Figure 6As shown, the scraping system includes a connecting block 201 and an arc-shaped piece 202; two front-to-back symmetrical sliding grooves 2002 are arranged on the placement block 2, and the placement groove 2001 is located between the two sliding grooves 2002; a connecting block 201 is slidably connected between the two sliding grooves 2002; two arc-shaped pieces 202 are movably connected to the connecting block 201, and the two arc-shaped pieces 202 are rotatably connected; each arc-shaped piece 202 is arranged to be a downwardly convex curved arc, and the lower side of the lower arc-shaped piece 202 is in contact with the surface of the placement groove 2001; a number of scraping blades 20201 are arranged on each arc-shaped piece 202; the fat tissue on the outer surface of the mouse intestine is scraped by the scraping blades 20201 on the two arc-shaped pieces 202 pressed together, thereby preventing the presence of fat tissue from affecting the accuracy of the sampling and detection results.

[0025] It also includes a baffle 203; the baffle 203 is fixedly connected to the concave part of the upper arc-shaped piece 202; the baffle 203 allows the scraped fat tissue to move to the right along the arc-shaped piece 202, thereby preventing the scraped fat tissue from being retained in the placement groove 2001, avoiding affecting the inspection personnel's observation of the outer surface of the mouse intestine, and improving the cleaning effect of the outer surface of the mouse intestine.

[0026] The cleaning of the outer surface of the mouse intestine is described in detail below: first, manually push the connecting block 201 so that the connecting block 201 is located at the left end of the placement block 2, and then put the mouse intestine cut into segments into the placement groove 2001. After fixing the left and right ends, rotate the upper arc piece 202 to close it with the lower arc piece 202. At this time, the mouse intestine is located between the arc pieces 202, and because the two arc pieces 202 are both set to be curved arcs convex downward, the mouse intestine is in a tightly fitted state with the two arc pieces 202. According to the size of the mouse intestine, use appropriate force to push the upper and lower arc pieces 202 together. The arc-shaped pieces 202 are pressed together, and then the connecting block 201 is pushed to slide to the right in the sliding groove 2002, so that the scraper 20201 on the pressed arc-shaped pieces 202 scrapes the fat tissue on the outer surface of the mouse intestine, thereby preventing the presence of fat tissue from affecting the accuracy of the sampling test results. At the same time, in the process of the two arc-shaped pieces 202 moving to the right, the scraped fat tissue is blocked by the baffle 203, thereby preventing the scraped fat tissue from remaining in the placement groove 2001, affecting the inspection personnel's observation of the outer surface of the mouse intestine, thereby improving the cleaning effect of the outer surface of the mouse intestine.

[0027] Example 3 On the basis of Example 2, Figure 7As shown, it also includes a semi-ring 301 and a push block 302; an arc-shaped block 4001 is arranged on the upper side of the second clamping block 4; a rotating block 4002 is arranged on the lower side of the second clamping block 4; a semi-ring 301 is fixedly connected to both the arc-shaped block 4001 and the rotating block 4002, and the two semi-rings 301 are attached together to form a complete circular ring; grooves are arranged on both the upper and lower sides of the second clamping block 4, and the semi-ring 301 slides in the grooves; a push block 302 is fixedly connected to the right side of the rotating block 4002 of the second clamping block 4; after the upper and lower parts of the second clamping block 4 are combined to fix the right end of the mouse, the tester pushes the push block 302 at the right end and makes the push block 302 rotate around the groove on the second clamping block 4, so that the whole mouse intestine rotates. At this time, sterile water flows through the mouse intestine, so the mouse intestine is in a bulging state, thus cooperating with the scraping blade 20201 on the arc-shaped piece 202 to scrape the adipose tissue at various parts on the outer surface of the mouse intestine, thereby further improving the cleaning effect of the outer surface of the mouse intestine.

[0028] Example 4 On the basis of Examples 1 - 3, as Figure 1 、 Figure 8 and Figure 9 shown, it also includes a curved arc piece 401 and a fixing block 404; five curved arc pieces 401 are fixedly connected to the right side of the extrusion rod 104, and each curved arc piece 401 is made of a deformable material; a fixing block 404 is fixedly connected to the right sides of the five curved arc pieces 401 together. When the extrusion rod 104 extrudes the contents of the mouse intestine, the fixing block 404 is extruded and moves leftward, thereby extruding the curved arc piece 401 and making it deform, gradually protruding towards the inner wall of the mouse intestine, realizing an increase in the contact area with the contents of the mouse intestine, and further improving the discharge effect of the contents inside the mouse intestine and the sampling efficiency.

[0029] It also includes ball bearings 402; four ball bearings 402 are rotatably connected to the middle of each curved arc piece 401; after the curved arc piece 401 deforms, it contacts the inner wall of the mouse intestine through the ball bearings 402 and scrapes the contents adhered to the inner wall, further improving the cleaning effect of the inner wall of the mouse intestine and avoiding incomplete cleaning, which may affect the subsequent sampling and detection results.

[0030] It also includes rubber sheets 403; a rubber sheet 403 is fixedly connected between every two adjacent curved arc pieces 401. Through the rubber sheet 403, the deformed curved arc pieces 401 are still in a closed state, preventing gaps from being generated after the curved arc piece 401 deforms and causing scratches on the inner wall of the mouse intestine during the scraping process, thus avoiding affecting the subsequent sampling and detection results.

[0031] The above has described the embodiments of the present invention in detail with reference to the accompanying drawings. However, the present invention is not limited to the above embodiments. Within the scope of knowledge possessed by those skilled in the art, various changes can be made without departing from the purpose of the present invention.

Claims

1. An Escherichia coli sampling and detection device for mouse experiments, comprising a sampling box (1); characterized in that, It also includes a fixing component, a placement block (2), a first clamping block (3), a second clamping block (4), an injection tube (5) and a scraping system; a fixing component is installed in the sampling box (1); a placement block (2) for placing the mouse intestine is arranged in the sampling box (1); a placement groove (2001) is arranged on the placement block (2); a first clamping block (3) for fixing the left end of the mouse intestine is slidably connected to the left side of the sampling box (1); a second clamping block (4) for fixing the right end of the mouse intestine is slidably connected to the right side of the sampling box (1); an injection tube (5) for flushing the inside of the mouse intestine is installed on the left side of the sampling box (1), and the injection tube (5) is arranged as a conical tube horizontally placed to the right; a scraping system for scraping the adipose tissue on the outer surface of the mouse intestine is arranged on the placement block (2).

2. The Escherichia coli sampling and detection device for mouse experiments according to claim 1, wherein The fixing component includes a fixing plate (101), a first fixing rod (102) and a second fixing rod (103); a fixing plate (101) is fixedly connected to the middle of the sampling box (1), and the lower side of the placement block (2) is fixedly connected to the fixing plate (101); a first fixing rod (102) is slidably connected to the left side of the sampling box (1), and the first clamping block (3) is fixedly connected to the first fixing rod (102); a second fixing rod (103) is slidably connected to the right side of the sampling box (1), and the second clamping block (4) is fixedly connected to the second fixing rod (103).

3. The Escherichia coli sampling and detection device for mouse experiments according to claim 1, characterized in that, It also includes a pressing rod (104); a pressing rod (104) for pressing the contents inside the mouse intestine is slidably connected to the left side of the sampling box (1), and the pressing rod (104) is located inside the injection tube (5).

4. The Escherichia coli sampling and detection device for mouse experiments according to claim 3, wherein, It also includes a fixing disk (105), an arc-shaped rod (106) and a limiting ring (107); a fixing disk (105) is slidably connected to the right side of the pressing rod (104); several round rods for restricting the movement of the fixing disk (105) are fixedly connected to the fixing disk (105); several arc-shaped rods (106) are fixedly connected to the fixing disk (105), and each arc-shaped rod (106) is made of a deformable material; several arc-shaped rods (106) are all located inside the injection tube (5); a limiting ring (107) is fixedly connected to the right side of the pressing rod (104), and the limiting ring (107) is located to the right of the fixing disk (105).

5. The Escherichia coli sampling and detection device for mouse experiments according to claim 1, characterized in that, The scraping system includes a connecting block (201) and an arc-shaped piece (202); several sliding grooves (2002) are arranged on the placement block (2), and the placement groove (2001) is located between several sliding grooves (2002); a connecting block (201) is slidably connected among several sliding grooves (2002); several arc-shaped pieces (202) are movably connected to the connecting block (201), and several arc-shaped pieces (202) are rotationally connected to each other; each arc-shaped piece (202) is arranged as a downwardly convex arc shape, and the lower side of the lower arc-shaped piece (202) is attached to the surface of the placement groove (2001); several scraping blades (20201) for scraping the adipose tissue on the outer surface of the mouse intestine are arranged on each arc-shaped piece (202).

6. The Escherichia coli sampling and detection device for mouse experiments according to claim 5, characterized in that, It also includes a baffle (203); a baffle (203) for preventing adipose tissue from remaining on the placement groove (2001) is fixedly connected to the concave part of the upper arc-shaped piece (202).

7. The Escherichia coli sampling and detection device for mouse experiments according to claim 1, wherein, It further includes a semi-ring (301) and a pushing block (302); an arc-shaped block (4001) is arranged on the upper side of the second clamping block (4); a rotating block (4002) is arranged on the lower side of the second clamping block (4); a semi-ring (301) is fixedly connected to both the arc-shaped block (4001) and the rotating block (4002), and the two semi-rings (301) are fitted together to form a complete circular ring; grooves are arranged on both the upper and lower sides of the second clamping block (4), and the semi-ring (301) slides in the grooves; a pushing block (302) for improving the cleaning effect of the outer surface of the mouse intestine is fixedly connected to the right side of the rotating block (4002) of the second clamping block (4).

8. The Escherichia coli sampling and detection device for mouse experiments according to claim 3, characterized in that, It further includes a curved arc plate (401) and a fixing block (404); a plurality of curved arc plates (401) for extruding the intestinal contents of the mouse are fixedly connected to the right side of the extrusion rod (104), and each curved arc plate (401) is made of a deformable material; a fixing block (404) is fixedly connected to the right sides of the plurality of curved arc plates (401) together.

9. The Escherichia coli sampling and detection device for mouse experiments according to claim 8, characterized in that, It further includes balls (402); a plurality of balls (402) are rotatably connected to the middle of each curved arc plate (401).

10. The Escherichia coli sampling and detection device for mouse experiments according to claim 8, characterized in that, It further includes a rubber sheet (403); a rubber sheet (403) is fixedly connected between every two adjacent curved arc plates (401).

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