Algae biotoxicity monitoring device

Abstract

The invention provides an algae biotoxicity detection device and a use method thereof.The detection device comprises a peristaltic pump, a metering unit, a multi-way valve, a chlorella solution tank, a distilled water tank, a water sample tank, a culture solution tank, a waste liquid tank, a control mechanism and a detection unit, and the detection unit comprises a shell, a culture tube A, a culture tube B and a constant-temperature control mechanism; the culture tube A and the culture tube B are respectively provided with a dissolved oxygen sensor and a light source group for providing illumination for photosynthesis of chlorella, and the culture tube A and the culture tube B are respectively connected with a multi-way valve through pipelines. According to the invention, the inhibition rate of the growth of chlorella is calculated by monitoring dissolved oxygen to realize on-line biotoxicity monitoring, and the device is simple in structure and high in detection precision.

Description

Technical Field

[0001] The present invention relates to an algal biotoxicity monitoring device, belonging to the technical field of ecological environment science and technology. Background Art

[0002] There are various types of toxic and harmful pollutants. The traditional physical and chemical monitoring methods that detect each single index one by one are beyond their capabilities and are difficult to meet the current needs of water environmental pollution risk monitoring and ecological status assessment under combined pollution. Therefore, it is necessary to conduct biological monitoring of pollutants to reflect the comprehensive toxicity of water bodies through monitoring.

[0003] Currently, water quality automatic online monitors are widely used in the field of water ecological environment monitoring. However, most of these devices are used for monitoring chemical indicators such as TP, TN, and COD. Their structures are complex, with numerous valves and pipelines and complex flow paths. Moreover, there are many dead volume areas in the flow path, and samples remain in the flow path, resulting in insufficient sample quantification accuracy and cross-interference between adjacent samples, directly affecting the accuracy of water quality biotoxicity measurement. In addition, most of these devices use acid-base buffer solution flushing to clean the pipelines, which changes the flow path environmental conditions and the cleaning is not thorough enough, directly affecting the photosynthetic activity state of the test algae. Moreover, algae can grow in the pipelines when the instrument is idle for a long time.

[0004] Chlorella is a spherical single-celled freshwater alga with a diameter of 3 - 8 micrometers. It is one of the earliest forms of life on Earth, appearing more than 2 billion years ago. It is widely distributed in nature, with the largest number of species in freshwater waters. Its cultivation is extremely simple and its adaptability is very strong. It is an efficient photosynthetic plant and an ideal test organism for online monitoring of water quality biotoxicity. Summary of the Invention

[0005] In view of the deficiencies of the prior art, the present invention provides an algal biotoxicity monitoring device that can effectively achieve online biotoxicity monitoring by monitoring the dissolved oxygen in the culture tube solution, with high detection accuracy and a simple structure.

[0006] To solve the above technical problems, the present invention provides an algal biotoxicity monitoring device, including a peristaltic pump, a metering unit, a multi-way valve, a Chlorella solution tank, a distilled water tank, a water sample tank, a culture solution tank, a waste liquid tank, a control mechanism, and a detection unit;

[0007] The peristaltic pump is connected to the metering unit, the metering unit is connected to the multi-way valve, and the Chlorella solution tank, the distilled water tank, the water sample tank, the culture solution tank, and the waste liquid tank are all connected to the multi-way valve;

[0008] The control mechanism is electrically connected to the peristaltic pump, the metering unit, the multi-way valve, and the detection unit;

[0009] The detection unit includes a housing, culture tube A, culture tube B, and a constant temperature control mechanism. Both ends of the top surface of the housing are provided with holes, and holes are also provided at positions corresponding to the positions of the holes at both ends of the top surface of the housing on the bottom surface of the housing. One end of culture tube A and one end of culture tube B respectively pass through the holes at both ends of the top surface of the housing and extend to the holes on the corresponding bottom surface, and then are connected to the bottom surface of the housing through the cooperation of a fixed seat and a hollow screw. The other ends of culture tube A and culture tube B are both connected to the top surface of the housing through the cooperation of a fixed seat and a screw. Both culture tube A and culture tube B are provided with dissolved oxygen sensors, and both culture tube A and culture tube B are respectively provided with a light source group for providing light for the photosynthesis of Chlorella. The constant temperature control mechanism is arranged in the inner cavity of the housing. Both culture tube A and culture tube B are connected to a multi-way valve through water pipes. The constant temperature control mechanism, the two light source groups, and the two dissolved oxygen sensors are all electrically connected to the control mechanism. The materials of both culture tube A and culture tube B are light-transmitting materials.

[0010] Preferably, the detection unit is further provided with an ultraviolet light mechanism. A hole is provided at the central position of the connection line of the holes at both ends of the top surface of the housing. The ultraviolet light source of the ultraviolet light mechanism passes through the hole at the central position of the connection line of the holes at both ends of the top surface of the housing and extends into the inner cavity of the housing. The lamp holder of the ultraviolet light mechanism is connected to the top surface of the housing through a fixed cover plate. The ultraviolet light mechanism is electrically connected to the control mechanism.

[0011] Preferably, the materials of both culture tube A and culture tube B are quartz.

[0012] Preferably, the light source group is a blue light source group.

[0013] Preferably, the multi-way valve is further connected to a solution tank for containing a toxic solution with known composition and concentration.

[0014] Preferably, one end of the water pipe connecting culture tube A and the multi-way valve passes through the hollow screw cooperating with the fixed seat at the bottom of culture tube A and extends into the inner cavity of culture tube A. One end of the water pipe connecting culture tube B and the multi-way valve passes through the hollow screw cooperating with the fixed seat at the bottom of culture tube B and extends into the inner cavity of culture tube B.

[0015] Preferably, the dissolved oxygen probe of the dissolved oxygen sensor of culture tube A passes through the fixed seat at the top of culture tube A and extends into the inner cavity of culture tube A. The dissolved oxygen probe of the dissolved oxygen sensor of culture tube B passes through the fixed seat at the top of culture tube B and extends into the inner cavity of culture tube B.

[0016] Preferably, sealing rings are arranged between both ends of culture tube A and the corresponding fixed seats, and sealing rings are arranged between both ends of culture tube B and the corresponding fixed seats.

[0017] Preferably, the materials of the fixed seat, the hollow screw, and the screw are all corrosion-resistant materials.

[0018] Accordingly, the present invention further provides a method for using an algal biotoxicity monitoring device, comprising the following steps:

[0019] Step 1: The control mechanism controls the multi-way valve, metering unit and peristaltic pump to add distilled water into culture tube A and culture tube B to clean culture tube A, culture tube B and the two dissolved oxygen probes. After the cleaning is completed, the control mechanism controls the multi-way valve, metering unit and peristaltic pump to introduce the waste liquid in culture tube A and culture tube B into the waste liquid tank, and then the control mechanism turns on the ultraviolet light mechanism to kill the algae or other microorganisms remaining on the culture tubes and dissolved oxygen probes;

[0020] Step 2: The control mechanism turns off the ultraviolet light mechanism and turns on the constant temperature control mechanism in the detection unit to keep the temperature inside the detection unit constant at 18°C;

[0021] Step 3: The control mechanism controls the multi-way valve, metering unit and peristaltic pump to add 2 mL of Chlorella solution and culture medium into culture tube A and culture tube B respectively;

[0022] Step 4: The control mechanism controls the multi-way valve, metering unit and peristaltic pump to add 2 mL of distilled water into culture tube A;

[0023] Step 5: The control mechanism controls the multi-way valve, metering unit and peristaltic pump to add 2 mL of water sample into culture tube B;

[0024] Step 6: The control mechanism reads and records the dissolved oxygen information of culture tube A and the dissolved oxygen information of culture tube B;

[0025] Step 7: The control mechanism turns on the two light source groups simultaneously and starts timing. When the irradiation duration of the light source group reaches the predetermined duration, the control mechanism turns off the two light source groups simultaneously;

[0026] Step 8: The control mechanism reads and records the dissolved oxygen information of culture tube A and the dissolved oxygen information of culture tube B again;

[0027] Step 9: After the dissolved oxygen information of culture tube A and culture tube B is read and recorded, the control mechanism controls the multi-way valve, metering unit and peristaltic pump to introduce the waste liquid in culture tube A and culture tube B into the waste liquid tank;

[0028] Step 10: After the waste liquid in culture tube A and culture tube B is drained, the control mechanism controls the multi-way valve, metering unit and peristaltic pump to add distilled water into culture tube A and culture tube B to clean culture tube A, culture tube B and the two dissolved oxygen probes. After the cleaning is completed, the control mechanism controls the multi-way valve, metering unit and peristaltic pump to introduce the waste liquid in culture tube A and culture tube B into the waste liquid tank, and then the control system turns on the ultraviolet light mechanism to kill the algae or other microorganisms remaining on the culture tubes and dissolved oxygen probes;

[0029] Step 11. The control mechanism automatically calculates and displays the inhibition rate I of the growth of Chlorella according to the following formula:

[0030] I = [(A2 - A1 - B2 + B1) / (A2 - A1)]·100%

[0031] Wherein, A1 is the dissolved oxygen value of the solution in culture tube A before turning on the light source group 45, A2 is the dissolved oxygen value of the solution in culture tube A after the Chlorella undergoes photosynthesis for a predetermined time after turning on the light source group, B1 is the dissolved oxygen value of the solution in culture tube B before turning on the light source group, and B2 is the dissolved oxygen value of the solution in culture tube B after the Chlorella undergoes photosynthesis for a predetermined time after turning on the light source group.

[0032] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0033] 1. The present invention controls the multi-way valve, metering unit, and peristaltic pump through the control mechanism to add Chlorella solution, distilled water, water sample, and culture solution into two culture tubes according to the actual situation. The constant temperature controller and the light source group provide suitable temperature and illumination for the photosynthesis of Chlorella under the control of the control mechanism, so as to stimulate the photosynthesis of Chlorella. The dissolved oxygen in the solutions in the two culture tubes is monitored by the dissolved oxygen sensor and transmitted to the control mechanism. The control mechanism stores the dissolved oxygen information and calculates and displays the inhibition rate of the growth of Chlorella according to the dissolved oxygen information, thereby realizing the on-line monitoring of biological toxicity. At the same time, through the real-time comparison and calibration of the water sample with the blank, the accuracy of each water sample test is ensured, and the monitoring accuracy is improved.

[0034] 2. The present invention uses distilled water to clean the culture tubes and the dissolved oxygen probe, and at the same time uses ultraviolet light to kill the algae or other microorganisms remaining on the culture tubes and the dissolved oxygen probe, reducing the interference of the cleaning procedure or the growth of algae or other microorganisms on the monitoring results of biological toxicity, and further improving the accuracy of the monitoring of biological toxicity.

[0035] 3. Since harmful substances in water will affect the growth of Chlorella, and the concentration of the poison is linearly negatively correlated with the oxygen production of Chlorella, the present invention determines the biological toxicity of the poison by monitoring the value of the dissolved oxygen in the culture tube and calculating the inhibition rate of the growth of Chlorella. Its structure is simple, the use cost is low, and the operation and maintenance are simple. Description of the Drawings

[0036] Figure 1 It is the structural layout diagram of the present invention.

[0037] Figure 2 It is the explosion structure schematic diagram of the detection unit of the present invention.

[0038] Reference numerals: peristaltic pump 1, metering unit 2, multi-way valve 3, detection unit 4, Chlorella solution tank 5, water sample tank 6, distilled water tank 7, culture solution tank 8, waste liquid tank 9, solution tank 10, housing 41, culture tube A 42, culture tube B 43, dissolved oxygen sensor 44, light source group 45, ultraviolet light mechanism 46, constant temperature control mechanism 47, fixed seat 48, hollow screw 49, sealing ring 50, ultraviolet light source 51, lamp holder 52, fixed cover plate 53, cover plate 54. Detailed implementation mode

[0039] The present invention will be described in detail below in combination with embodiments and the accompanying drawings. It should be noted that, without conflict, the embodiments in the present invention and the features in the embodiments can be combined with each other.

[0040] Refer to Figures 1 to 2 , an algal biotoxicity monitoring device, comprising a peristaltic pump 1, a metering unit 2, a multi-way valve 3, a Chlorella solution tank 5, a distilled water tank 7, a water sample tank 6, a culture solution tank 8, a waste liquid tank 10, a control mechanism and a detection unit 4;

[0041] The peristaltic pump 1 is connected to the metering unit 2, the metering unit 2 is connected to the multi-way valve 3, and the Chlorella solution tank 5, the distilled water tank 7, the water sample tank 6, the culture solution tank 8 and the waste liquid tank 10 are all connected to the multi-way valve 3;

[0042] The control mechanism is electrically connected to the peristaltic pump 1, the metering unit 2, the multi-way valve 3 and the detection unit 4;

[0043] The detection unit 4 includes a housing 41, a culture tube A 42, a culture tube B 43 and a constant temperature control mechanism 47. The culture tube A 42 and the culture tube B 43 are respectively filled with a water sample solution and a distilled water solution to compare and proofread the water sample with the blank. The housing 4 is provided with a cover plate 54. Holes are opened at both ends of the top surface of the housing 41, and holes are also opened at positions corresponding to the positions of the holes at both ends of the top surface of the housing 41 on the bottom surface of the housing 41. One end of the culture tube A 42 and one end of the culture tube B 43 respectively pass through the holes at both ends of the top surface of the housing 41 and extend to the corresponding holes on the bottom surface, and then are connected to the bottom surface of the housing 41 through the cooperation of a fixing seat 48 and a hollow screw 49. The other ends of the culture tube A 42 and the culture tube B 43 are both connected to the top surface of the housing 41 through the cooperation of a fixing seat 48 and a screw. The upper ends of the culture tube A 42 and the culture tube B 43 are not sealed, and air can enter the culture tube A 42 and the culture tube B 43. Both the culture tube A 42 and the culture tube B 43 are provided with dissolved oxygen sensors 44. Preferably, the dissolved oxygen probe of the dissolved oxygen sensor 44 of the culture tube A 42 passes through the fixing seat 48 at the top of the culture tube A 42 and extends into the inner cavity of the culture tube A 42. The dissolved oxygen probe of the dissolved oxygen sensor 44 of the culture tube B 43 passes through the fixing seat 48 at the top of the culture tube B 43 and extends into the inner cavity of the culture tube B 43. The end parts of the two dissolved oxygen probes respectively extend below the liquid levels of the culture tube A 42 and the culture tube B 43 to effectively detect the dissolved oxygen in the solutions in the culture tube A 42 and the culture tube B 43. The culture tube A 42 and the culture tube B 43 are respectively provided with a light source group 45 for providing light for the photosynthesis of Chlorella. The constant temperature control mechanism 47 is arranged in the inner cavity of the housing 41. The constant temperature control mechanism 47 provides a suitable temperature for Chlorella under the control of the control mechanism, preferably 18°C. The culture tube A 42 and the culture tube B 43 are both connected to the multi-way valve 3 through water pipes. The constant temperature control mechanism 47, the two light source groups 45 and the two dissolved oxygen sensors 44 are all electrically connected to the control mechanism. The materials of the culture tube A 42 and the culture tube B 43 are both light-transmitting materials. Optionally, the materials of the culture tube A 42 and the culture tube B 43 are both quartz. The light of the light source group 45 can pass through the walls of the culture tube A 42 and the culture tube B 43 to provide light for the photosynthesis of Chlorella, and the light duration can be set according to the actual situation.

[0044] Under suitable temperature and light, Chlorella will carry out photosynthesis and produce oxygen. When the water body is safe, the photosynthesis of Chlorella is strong and a large amount of oxygen is produced. When the water body is polluted, the activity of Chlorella decreases, the photosynthesis declines, and the amount of oxygen produced decreases. When the pollution is serious, Chlorella will even die.

[0045] Through the dissolved oxygen value monitored by the dissolved oxygen sensor, the control mechanism substitutes the dissolved oxygen value into the formula to calculate the inhibition rate of the growth of Chlorella and can display it, so as to reflect the biotoxicity of the specific water sample.

[0046] Preferably, the detection unit 4 is further provided with an ultraviolet light mechanism 46. A hole is opened at the center position of the connection line of the holes at both ends of the top surface of the housing 41. The ultraviolet light source 51 of the ultraviolet light mechanism 46 extends through the hole at the center position of the connection line of the holes at both ends of the top surface of the housing 41 into the inner cavity of the housing 41. The lamp holder 52 of the ultraviolet light mechanism 46 is connected to the top surface of the housing 41 through a fixed cover plate 53. The ultraviolet light mechanism 46 is electrically connected to the control mechanism, and ultraviolet light is used to kill algae or other microorganisms remaining in the culture tube and the dissolved oxygen probe under the control of the control mechanism.

[0047] Preferably, the light source group 45 is a blue light source group, which provides light for better stimulating the photosynthesis of Chlorella.

[0048] Optionally, the multi-way valve 3 is further connected to a solution tank 10 for containing a toxic solution with known components and concentrations, so as to determine the concentration of the toxic solution corresponding to the growth inhibition rate of Chlorella. When the components of the toxic solution are known, after the dissolved oxygen data is measured by the detection structure, the growth inhibition rate of Chlorella can be calculated by a formula, and the concentration of the toxic solution can be obtained.

[0049] One end of the water pipe connecting the culture tube A 42 and the multi-way valve 3 passes through a hollow screw 49 that cooperates with the fixed seat 48 at the bottom of the culture tube A 42 and extends into the inner cavity of the culture tube A 42. One end of the water pipe connecting the culture tube B 43 and the multi-way valve 3 passes through a hollow screw 49 that cooperates with the fixed seat 48 at the bottom of the culture tube B 43 and extends into the inner cavity of the culture tube B 43, which facilitates the entry of Chlorella solution, distilled water, water sample, and culture solution into the culture tube A 42 and the culture tube B 43 under the control of the control mechanism, and the unnecessary solution in the culture tube A 42 and the culture tube B 43 enters the waste liquid tank 9 under the control of the control mechanism.

[0050] Preferably, sealing rings 50 are provided between both ends of the culture tube A 42 and the corresponding fixed seats 48, and sealing rings 50 are provided between both ends of the culture tube B 43 and the corresponding fixed seats 48 to prevent damage to the culture tube A 42 and the culture tube B 43.

[0051] The fixed seats 48, the hollow screws 49, and the screws are all made of corrosion-resistant materials to improve their service life.

[0052] Correspondingly, a method for using an algal biotoxicity monitoring device includes the following operation steps:

[0053] 1. The control mechanism controls the multi-way valve 3, the metering unit 2, and the peristaltic pump 1 to add distilled water into the culture tube A42 and the culture tube B43 to clean the culture tube A42, the culture tube B43, and the two dissolved oxygen probes. After the cleaning is completed, the control mechanism controls the multi-way valve 3, the metering unit 2, and the peristaltic pump 1 to introduce the waste liquid in the culture tube A42 and the culture tube B43 into the waste liquid tank 9, and then the control mechanism turns on the ultraviolet light mechanism 46 to kill the algae or other microorganisms remaining on the culture tubes and the dissolved oxygen probes.

[0054] 2. The control mechanism turns off the ultraviolet light mechanism 46 and turns on the constant temperature control mechanism 47 in the detection unit 4 to keep the temperature in the inner cavity of the detection unit 4 constant at 18 °C.

[0055] 3. The control mechanism controls the multi-way valve 3, the metering unit 2, and the peristaltic pump 1 to add 2 mL of Chlorella solution and culture medium into the culture tube A42 and the culture tube B43 respectively.

[0056] 4. The control mechanism controls the multi-way valve 3, the metering unit 2, and the peristaltic pump 1 to add 2 mL of distilled water into the culture tube A42.

[0057] 5. The control mechanism controls the multi-way valve 3, the metering unit 2, and the peristaltic pump 1 to add 2 mL of water sample into the culture tube B43.

[0058] 6. The control mechanism reads and records the dissolved oxygen information of the culture tube A42 and the dissolved oxygen information of the culture tube B43.

[0059] 7. The control mechanism turns on the two groups of light source groups 45 simultaneously and starts timing. When the irradiation duration of the light source group 45 reaches the predetermined duration, the control mechanism turns off the two groups of light source groups 45 simultaneously. Preferably, the predetermined duration is 15 minutes.

[0060] 8. The control mechanism reads and records the dissolved oxygen information of the culture tube A42 and the dissolved oxygen information of the culture tube B43 again.

[0061] 9. After the dissolved oxygen information of the culture tube A42 and the culture tube B43 is read and recorded, the control mechanism controls the multi-way valve 1, the metering unit 2, and the peristaltic pump 3 to introduce the waste liquid in the culture tube A42 and the culture tube B43 into the waste liquid tank 9.

[0062] 10. After the waste liquid in culture tubes A42 and B43 is drained completely, the control mechanism controls the multi-way valve 3, metering unit 2 and peristaltic pump 1 to add distilled water into culture tubes A42 and B43 to clean culture tubes A42, B43 and the two dissolved oxygen probes. After the cleaning is completed, the control mechanism controls the multi-way valve 3, metering unit 2 and peristaltic pump 1 to introduce the waste liquid in culture tubes A42 and B43 into the waste liquid tank 9, and then starts the ultraviolet light mechanism 46 through the control system to kill the algae or other microorganisms remaining on the culture tubes and dissolved oxygen probes.

[0063] 11. The control mechanism automatically calculates and displays the inhibition rate I of the growth of Chlorella according to the following formula:

[0064] I = [(A2 - A1 - B2 + B1) / (A2 - A1)]·100%

[0065] Wherein, A1 is the dissolved oxygen value of the solution in culture tube A before the light source group 45 is turned on, A2 is the dissolved oxygen value of the solution in culture tube A after the Chlorella has carried out photosynthesis for a preset time after the light source group 45 is turned on, B1 is the dissolved oxygen value of the solution in culture tube B before the light source group 45 is turned on, and B2 is the dissolved oxygen value of the solution in culture tube B after the Chlorella has carried out photosynthesis for a preset time after the light source group 45 is turned on.

[0066] By detecting the dissolved oxygen values of several water samples through the above steps and calculating the inhibition rate I of the growth of Chlorella according to the formula based on the dissolved oxygen values, the biotoxicity of the water sample can be reflected.

[0067] Using this device to monitor the inhibition rate of the growth of Chlorella by a potassium dichromate solution with a concentration of 0.8 mg / L according to the corresponding usage steps (monitoring 6 times), adding distilled water to culture tube A and adding a potassium dichromate solution with a concentration of 0.8 mg / L to culture tube B, the situation is shown in Table 1:

[0068] Table 1 Inhibition rate of the growth of Chlorella by a potassium dichromate solution with a concentration of 0.8 mg / L

[0069]

[0070] It can be seen from the data in Table 1 that the inhibition rate of the growth of Chlorella is between 50% and 54%, the test data is stable, and it conforms to the conclusion of "Water Quality - Freshwater Algal Growth Inhibition Test with Unicellular Green Algae" with the standard number BS EN ISO 8692 - 2012.

[0071] Using this device, the concentration of the toxic solution with known components corresponding to the growth inhibition rate of Chlorella can be determined. When the components of the known toxic solution are known, after the dissolved oxygen data is measured by the detection structure, the growth inhibition rate of Chlorella can be calculated through a formula, and then the concentration of the toxic solution can be obtained. For example: Using this device, monitor the growth inhibition rate of different concentrations of potassium dichromate solution on Chlorella according to the corresponding usage steps. Each time, add distilled water to culture tube A and potassium dichromate solution to culture tube B. The situation is shown in Table 2 as follows:

[0072] Table 2 Growth inhibition rate of different concentrations of potassium dichromate solution on Chlorella

[0073]

[0074] From the data in Table 2, it can be known that when the concentration of the potassium dichromate test solution is 0.8 mg / L, the growth inhibition rate of Chlorella is closest to 50%.

[0075] Using this device, monitor the growth inhibition rate of distilled water on Chlorella (monitor 6 times) according to the corresponding usage steps. Add distilled water to both culture tube A and culture tube B. The situation is shown in Table 3 as follows:

[0076] Table 3 Growth inhibition rate of distilled water on Chlorella

[0077]

[0078] From the data in Table 3, it can be seen that the growth inhibition rate of Chlorella is between -10% and 10%, and the data is stable.

[0079] By analyzing the above data, the monitoring results of the device of the present invention can meet the technical requirements of on-line monitoring of water quality biotoxicity and can effectively monitor the biotoxicity of water quality.

[0080] The above content is a further detailed description of the present invention in combination with specific preferred embodiments. It cannot be determined that the specific implementation of the present invention is only limited to these descriptions. For those of ordinary skill in the technical field to which the present invention belongs, without departing from the concept of the present invention, several simple deductions and substitutions can still be made, and all should be regarded as belonging to the protection scope of the present invention.

Claims

1. An algal biotoxicity monitoring device, characterized in that, It includes a peristaltic pump (1), a metering unit (2), a multi-way valve (3), a Chlorella solution tank (5), a distilled water tank (7), a water sample tank (6), a culture solution tank (8), a waste liquid tank (9), a control mechanism and a detection unit (4); The peristaltic pump (1) is connected to the metering unit (2), the metering unit (2) is connected to the valve core of the multi-way valve (3), and the Chlorella solution tank (5), the distilled water tank (7), the water sample tank (6), the culture solution tank (8) and the waste liquid tank (9) are all connected to the corresponding channels of the multi-way valve (3) through pipelines; The control mechanism is electrically connected to the peristaltic pump (1), the metering unit (2), the multi-way valve (3) and the detection unit (4); The detection unit (4) includes a housing (41), a culture tube A (42), a culture tube B (43) and a constant temperature control mechanism (47). There are holes at both ends of the top surface of the housing (41), and holes are also provided at positions corresponding to the positions of the holes at both ends of the top surface of the housing at the bottom surface of the housing (41). One end of the culture tube A (42) and one end of the culture tube B (43) respectively pass through the holes at both ends of the top surface of the housing and extend to the holes at the corresponding bottom surface, and then are connected to the bottom surface of the housing (41) through the cooperation of a fixing seat (48) and a hollow screw (49). The other ends of the culture tube A (42) and the culture tube B (43) are both connected to the top surface of the housing (41) through the cooperation of a fixing seat (48) and a screw. The culture tube A (42) and the culture tube B (43) are both provided with dissolved oxygen sensors (44). The culture tube A (42) and the culture tube B (43) are respectively provided with a light source group (45) for providing light for the photosynthesis of Chlorella. The constant temperature control mechanism (47) is arranged in the inner cavity of the housing (41). The culture tube A (42) and the culture tube B (43) are both connected to the corresponding channels of the multi-way valve (3) through pipelines. The constant temperature control mechanism (47), the two light source groups (45) and the two dissolved oxygen sensors (44) are all electrically connected to the control mechanism. The materials of the culture tube A (42) and the culture tube B (43) are both light-transmitting materials.

2. The algal biotoxicity monitoring device according to claim 1, characterized in that, The detection unit (4) is further provided with an ultraviolet light mechanism (46). There is a hole at the central position of the connection line of the holes at both ends of the top surface of the housing (41). The ultraviolet light source (51) of the ultraviolet light mechanism (46) passes through the hole at the central position of the connection line of the holes at both ends of the top surface of the housing (41) and extends into the inner cavity of the housing (41). The lamp holder (52) of the ultraviolet light mechanism (46) is connected to the top surface of the housing (41) through a fixed cover plate (53). The ultraviolet light mechanism (46) is electrically connected to the control mechanism.

3. The algal biotoxicity monitoring device according to claim 2, wherein The materials of the culture tube A (42) and the culture tube B (43) are both quartz.

4. The algal biotoxicity monitoring device according to claim 3, wherein The light source group (45) is a blue light source group.

5. The algal biotoxicity monitoring device according to claim 4, wherein The multi-way valve (3) is further connected to a solution tank (10) for containing a toxic solution with known composition and concentration.

6. The algal biotoxicity monitoring device according to claim 5, wherein, One end of the water pipe connecting the culture tube A (42) and the multi-way valve (3) passes through a hollow screw (49) that mates with a fixed seat (48) at the bottom of the culture tube A (42) and extends into the inner cavity of the culture tube A (42). One end of the water pipe connecting the culture tube B (43) and the multi-way valve (3) passes through a hollow screw (49) that mates with a fixed seat (48) at the bottom of the culture tube B (43) and extends into the inner cavity of the culture tube B.

7. The algal biotoxicity monitoring device according to claim 6, wherein The dissolved oxygen probe of the dissolved oxygen sensor (44) of the culture tube A (42) passes through a fixed seat (48) at the top of the culture tube A (42) and extends into the inner cavity of the culture tube A (42). The dissolved oxygen probe of the dissolved oxygen sensor (44) of the culture tube B (43) passes through a fixed seat (48) at the top of the culture tube B (43) and extends into the inner cavity of the culture tube B (43).

8. The algal biotoxicity monitoring device according to claim 7, wherein, Sealing rings (50) are provided between both ends of the culture tube A (42) and the corresponding fixed seats (48). Sealing rings (50) are provided between both ends of the culture tube B (43) and the corresponding fixed seats (48).

9. The algal biotoxicity monitoring device according to claim 8, characterized in that, The fixed seat (48), the hollow screw (49), and the screw are all made of corrosion-resistant materials.

10. A method for using an algal biotoxicity monitoring device, characterized in that, It includes the following steps: Step 1: The control mechanism controls the multi-way valve (3), the metering unit (2), and the peristaltic pump (1) to add distilled water into the culture tube A (42) and the culture tube B (43) to clean the culture tube A (42), the culture tube B (43), and the two dissolved oxygen probes. After the cleaning is completed, the control mechanism controls the multi-way valve (3), the metering unit (2), and the peristaltic pump (1) to introduce the waste liquid in the culture tube A (42) and the culture tube B (43) into the waste liquid tank (9), and then the control mechanism turns on the ultraviolet light mechanism (46) to kill the algae or other microorganisms remaining on the culture tube and the dissolved oxygen probes. Step 2: The control mechanism turns off the ultraviolet light mechanism (46), and the control mechanism turns on the temperature control mechanism (47) in the detection unit (4) to keep the temperature in the inner cavity of the detection unit (4) constant at 18 °C. Step 3: The control mechanism controls the multi-way valve (3), the metering unit (2), and the peristaltic pump (1) to add 2 mL of Chlorella solution and culture medium into the culture tube A (42) and the culture tube B (43) respectively. Step 4: The control mechanism controls the multi-way valve (3), the metering unit (2), and the peristaltic pump (1) to add 2 mL of distilled water into the culture tube A (42). Step 5: The control mechanism controls the multi-way valve (3), the metering unit (2), and the peristaltic pump (1) to add 2 mL of water sample into the culture tube B (43). Step 6: The control mechanism reads and records the dissolved oxygen information of the culture tube A (42) and the dissolved oxygen information of the culture tube B (43). Step 7: The control mechanism turns on both groups of light source groups (45) simultaneously and starts timing. When the irradiation duration of the light source group (45) reaches the predetermined duration, the control mechanism turns off both groups of light source groups (45) simultaneously. Step 8: The control mechanism reads and records the dissolved oxygen information of the culture tube A (42) and the dissolved oxygen information of the culture tube B (43) again. Step 9: After the dissolved oxygen information of culture tube A (42) and culture tube B (43) is read and recorded, the control mechanism controls the multi-way valve (3), metering unit (2) and peristaltic pump (1) to introduce the waste liquid in culture tube A (42) and culture tube B (43) into the waste liquid tank (9). Step 10: After the waste liquid in culture tube A (42) and culture tube B (43) is drained, the control mechanism controls the multi-way valve (3), metering unit (2) and peristaltic pump (1) to add distilled water into culture tube A (42) and culture tube B (43) to clean culture tube A (42), culture tube B (43) and the two dissolved oxygen probes. After the cleaning is completed, the control mechanism controls the multi-way valve (3), metering unit (2) and peristaltic pump (1) to introduce the waste liquid in culture tube A (42) and culture tube B (43) into the waste liquid tank (9), and then turns on the ultraviolet light mechanism (46) through the control system to kill the algae or other microorganisms remaining on the culture tubes and dissolved oxygen probes. Step 11: The control mechanism automatically calculates and displays the inhibition rate I of the growth of Chlorella according to the following formula: I = [(A2 - A1 - B2 + B1) / (A2 - A1)]·100% where A1 is the dissolved oxygen value of the solution in culture tube A (42) before turning on the light source group (45), A2 is the dissolved oxygen value of the solution in culture tube A (42) after the Chlorella has carried out photosynthesis for a predetermined period of time after turning on the light source group (45), B1 is the dissolved oxygen value of the solution in culture tube B (43) before turning on the light source group (45), and B2 is the dissolved oxygen value of the solution in culture tube B (43) after the Chlorella has carried out photosynthesis for a predetermined period of time after turning on the light source group (45).

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