Devices and methods for modifying trichothecenes

Abstract

The present invention relates to a method for modifying trichothecene, a composition comprising at least one polypeptide having at least 70% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO. 1 to SEQ ID NO. 54, and the use of said polypeptide.

Description

[0001] The present invention relates to a method for modifying at least one trichothecene, a composition comprising at least one polypeptide, and the use of said polypeptide.

[0002] Mycotoxins are secondary metabolites produced by various fungal species. Such fungi infect food and feed materials, especially all types of grains and feed crops, or other commodities such as cotton, coffee, peanuts, dates, spices, etc. When the fungi grow, mycotoxins such as aflatoxins, ochratoxins, ergot alkaloids, fumonisins, zearalenone and / or trichothecenes are produced, thus contaminating the infected materials.

[0003] In more than 17,000 samples, almost three quarters were found to contain at least one mycotoxin, and more than half of all samples contained one or more trichothecenes (Streit et al., 2013. J Sci Food Agric. 93(12):2892 - 2899). Deoxynivalenol (DON; also known as vomitoxin; CAS No. 51481 - 10 - 8) is one of the most prevalent trichothecenes. Feed contaminated with DON causes feed refusal and reduced weight gain, and thus not only affects animal health and well - being, but also causes significant economic losses in the livestock industry.

[0004] To some extent, the degree of contamination can be reduced by maintaining "good agricultural practices", by avoiding humidity as much as possible to inhibit fungal growth, or by applying fungicides to the infected materials. In addition, contaminated materials should be removed and discarded before processing. However, due to the high prevalence and low median toxic dose, further measures must be considered.

[0005] The conversion of DON to its isomer 3-epi-DON has been described as an effective measure for DON detoxification (Bracarense et al., 2020. Food Chem Toxicol. 140:111241). In the case of DON, the hydroxyl group at carbon atom 3 is in the R configuration, while in the case of 3-epi-DON, the hydroxyl group at carbon atom 3 is in the S configuration. The detoxification conversion from DON to 3-epi-DON has previously been described as a two-step mechanism through the intermediate trichothecene 3-keto-DON (Carere et al 2018. Microb Biotechnol. 11(6):1106-1111; Carere et al 2018. Front Microbiol. 9:1573; He et al 2020. Food Chem. 321:126703). These described mechanisms rely on a pyrroloquinoline quinone (PQQ)-dependent dehydrogenase to oxidize DON to form 3-keto-DON with a carbonyl group at carbon 3, and on one or more reductases to reduce 3-keto-DON to 3-epi-DON. It should be noted that some enzymes capable of using 3-keto-DON as a substrate catalyze reduction reactions that at least partially result in the return of DON rather than the detoxified isomer 3-epi-DON. Additionally, the oxidation reaction requires free PQQ, and the reduction reaction depends on the nicotinamide cofactor NADPH (reduced nicotinamide adenine dinucleotide phosphate). Although these reactions may be very efficient, the dependence on two or even three enzymes and at least two additional substrates PQQ and NADPH makes these mechanisms rather complex and thus difficult to apply as, for example, detoxifying additives for food or feed. Furthermore, NADPH is an acid-labile and relatively unstable molecule that readily oxidizes rapidly and must be supplied as a constant, continuous substance or regenerated through a cofactor regeneration system to ensure that the entire epimerization proceeds within the desired field. Such a cofactor regeneration system can consist of at least one enzyme that uses the oxidized cofactor NADP + to regenerate NADPH. It should be noted that due to the limited availability and stability of NADP(H), the in vivo application of such systems is challenging. Thus, although these published mechanisms ultimately result in 3-epi-DON, they are still accompanied by several obstacles in terms of applicability. Specifically, these published mechanisms rely on several cofactors and require a large number of enzymes.

[0006] In view of the prior art as described above, the object of the present invention is to provide devices and methods for modifying trichothecenes such as DON or 3-keto-DON to form less toxic trichothecene derivatives.

[0007] This object is achieved by providing a method for modifying at least one trichothecene (e.g., detoxifying at least one trichothecene), the method comprising the step of forming a reaction mixture by contacting at least one polypeptide with a nutritional composition comprising at least one trichothecene, and with NAD(H) and / or NADP(H), preferably with NAD(H), wherein the at least one polypeptide comprises an amino acid sequence having at least 70% sequence identity with an amino acid sequence selected from SEQ ID NO.1 to SEQ ID NO.54, preferably selected from SEQ ID NO.1 and SEQ ID NO.34. When performing such a method, detoxification of trichothecene can be achieved. Specifically, detoxification of DON to form less toxic 3-keto-DON, and / or modification of 3-keto-DON to form less toxic 3-epi-DON can be achieved.

[0008] As used herein, a nutritional composition is a composition comprising one or more nutritionally valuable components. Such components tend to provide energy to the consumer of the nutritional composition. The nutritional composition can be wholly or at least partly based on herbs or plants, such as common animal feed compositions. In a preferred embodiment, the at least one polypeptide is included in the reaction mixture at a concentration of at least 0.1 mM. Most preferably, the at least one polypeptide is included in the reaction mixture at a concentration of at least 1 mM. In some embodiments, the at least one polypeptide is included in the reaction mixture at a concentration of 1.0 mM, 1.1 mM, 1.2 mM, 1.3 mM, 1.4 mM or 1.5 mM.

[0009] The term "polypeptide" should be construed in the manner commonly used in the art and encompasses, for example, polypeptides, proteins, peptides, enzymes.

[0010] The term "sequence identity" is used to describe the degree of relatedness between two or more nucleic acid sequences (e.g., DNA or RNA polynucleotides) contained in a polynucleotide, or between two or more amino acid sequences contained in a polypeptide. Sequence identity can be determined by conventional methods known to those skilled in the art. In the present disclosure, a preferred method for determining the sequence identity between two amino acid sequences contained in two polypeptides is to use the Clustal Omega alignment tool of EMBL-EBI with default settings (https: / / www.ebi.ac.uk / Tools / msa / clustalo / ; Sievers et al. 2011. Mol. Syst. Biol. 7:539). Alternatively, the Needleman-Wunsch algorithm for global sequence alignment can be used with default settings (gap cost: existence: 11 extensions 1), such as the algorithm provided by the National Center for Biotechnology Information in the United States ("Needleman-Wunsch Global Align Protein Sequences"). A polypeptide containing an amino acid sequence having at least 70% sequence identity with the amino acid sequence of a target polypeptide can be a polypeptide containing an amino acid sequence having 70% or more sequence identity with the amino acid sequence of the target polypeptide, such as 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or even 100% sequence identity. For example, a polypeptide containing an amino acid sequence having 100% sequence identity with SEQ ID NO.1 is encompassed by the group of "polypeptides containing an amino acid sequence having at least 70% sequence identity with the amino acid sequence of SEQ ID NO.1". A common practice in the art is to form a fusion polypeptide by linking one or more peptide tags to the N-terminus and / or C-terminus of a polypeptide, for example, to facilitate easier purification or increase solubility, while substantially not altering the enzymatic properties of the labeled polypeptide. Generally, the term "fusion polypeptide" or "fusion protein" describes two or more polypeptide chains that are usually linked to each other by peptide bonds. Examples of commonly used tags include poly(His) tags (e.g., hexahistidine tags), maltose-binding protein (MBP) tags, Strep tags, StrepII tags, etc. Accordingly, the present invention encompasses fusion polypeptides having enzymatic activity including polypeptides containing an amino acid sequence having at least 70% sequence identity with the amino acid sequences selected from SEQ ID NO.1 to SEQ ID NO.54, preferably selected from SEQ ID NO.1 and SEQ ID NO.34.Such fusion polypeptides can for example comprise a hexahistidine tag fused to the N-terminus of a polypeptide comprising an amino acid sequence having 91% sequence identity with the amino acid sequence of SEQ ID NO.1, or for example comprise a maltose binding protein tag fused to the C-terminus of a polypeptide comprising an amino acid sequence having 100% sequence identity with the amino acid sequence of SEQ ID NO.34 via a linker peptide (such as GG; GPG; EAEA; GGSG; GGGGS; GSPAG; etc.). In other words, the present invention also encompasses said larger polypeptides comprising an amino acid sequence having at least 70% sequence identity with an amino acid sequence selected from SEQ ID NO.1 to SEQ ID NO.54, preferably selected from SEQ ID NO.1 and SEQ ID NO.34 as part of the larger polypeptide, provided that the enzymatic activity of the amino acid sequence having at least 70% sequence identity with an amino acid sequence selected from SEQ ID NO.1 to SEQ ID NO.54, preferably selected from SEQ ID NO.1 and SEQ ID NO.34 is not substantially eliminated. For the sake of clarity only, the polypeptides with enzymatic activity according to the present invention are capable of and thus suitable for modifying at least one trichothene.

[0011] "NAD(H)" refers to the oxidized form of the cofactor nicotinamide adenine dinucleotide, i.e. NAD + , and the reduced form, i.e. NADH. "NADP(H)" refers to the oxidized form of the cofactor nicotinamide adenine dinucleotide phosphate, i.e. NADP + , and the reduced form, i.e. NADPH. In a preferred embodiment of the method, the cofactor to which at least one polypeptide and the nutritional composition are contacted is NAD + and / or NADH.

[0012] In some embodiments of the present invention, the at least one trichothene is deoxynivalenol and / or 3-keto-deoxynivalenol.

[0013] In some embodiments, the method according to the invention comprises the step of incubating the reaction mixture at a temperature between 5 °C and 40 °C, preferably at a temperature between 20 °C and 40 °C. In a preferred embodiment, the reaction mixture is incubated at body temperature, for example between 35 °C and 40 °C. Preferably, the step of incubating the reaction mixture is carried out for at least 5 minutes, preferably at least 10 min (e.g. at least 15 min, 30 min, 60 min, etc.). The longer the step of incubating the reaction mixture is carried out, the greater the degree of modification of the trichothecene. Nevertheless, those skilled in the art know that the enzymatic type of reaction as described herein can also be carried out outside of said preferred conditions. For the sake of clarity only, it is considered that the incubation starts immediately after the polypeptide according to the invention has come into contact with the nutritional composition comprising at least one trichothecene. Those skilled in the art are well able to select appropriate incubation conditions to vary the concentration of trichothecene in the composition to the desired extent.

[0014] In some embodiments, the at least one trichothene is deoxynivalenol, and the at least one polypeptide comprises an amino acid sequence selected from SEQ ID NO.1 to SEQ ID NO.33 (e.g., SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9, SEQ ID NO.10, SEQ ID NO.11, SEQ ID NO.12, SEQ ID NO.13, SEQ ID NO.14, SEQ ID NO.15, SEQ ID NO.16, SEQ ID NO.17, SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.21, SEQ ID NO.22, SEQ ID NO.23, SEQ ID NO.24, SEQ ID NO.25, SEQ ID NO.26, SEQ ID NO.27, SEQ ID NO.28, SEQ ID NO.29, SEQ ID NO.30, SEQ ID NO.31, SEQ ID NO.32, SEQ ID NO.33, SEQ ID NO.1 to SEQ ID NO.2, SEQ ID NO.1 to SEQ ID NO.3, SEQ ID NO.1 to SEQ ID NO.4, SEQ ID NO.1 to SEQ ID NO.5, SEQ ID NO.1 to SEQ ID NO.6, SEQ ID NO.1 to SEQ ID NO.7, SEQ ID NO.1 to SEQ ID NO.8, SEQ ID NO.1 to SEQ ID NO.9, SEQ ID NO.1 to SEQ ID NO.10, SEQ ID NO.1 to SEQ ID NO.11, SEQ ID NO.1 to SEQ ID NO.12, SEQ ID NO.1 to SEQ ID NO.13, SEQ ID NO.1 to SEQ ID NO.14, SEQ ID NO.1 to SEQ ID NO.15, SEQ ID NO.1 to SEQ ID NO.16, SEQ ID NO.1 to SEQ ID NO.17, SEQ ID NO.1 to SEQ ID NO.18, SEQ ID NO.1 to SEQ ID NO.19, SEQ ID NO.1 to SEQ ID NO.20, SEQ ID NO.1 to SEQ ID NO.21, SEQ ID NO.1 to SEQ ID NO.22. The amino acid sequences of SEQ ID NO.1 to SEQ ID NO.23, SEQ ID NO.1 to SEQ ID NO.24, SEQ ID NO.1 to SEQ ID NO.25, SEQ ID NO.1 to SEQ ID NO.26, SEQ ID NO.1 to SEQ ID NO.27, SEQ ID NO.1 to SEQ ID NO.28, SEQ ID NO.1 to SEQ ID NO.29, SEQ ID NO.1 to SEQ ID NO.30, SEQ ID NO.1 to SEQ ID NO.31, SEQ ID NO.1 to SEQ ID NO.32, and / or SEQ ID NO.1 to SEQ ID NO.33), preferably amino acid sequences having at least 70% sequence identity with the amino acid sequence of SEQ ID NO.1. Specifically, the at least one trichothene is deoxynivalenol, and the at least one polypeptide has at least 70% sequence identity with an amino acid sequence selected from SEQ ID NO.1 to SEQ ID NO.33, preferably with the amino acid sequence of SEQ ID NO.1. Such a polypeptide is particularly effective in converting DON to 3-keto-DON.

[0015] In some embodiments, the at least one trichothene is 3-keto-deoxynivalenol, and the at least one polypeptide comprises an amino acid sequence selected from SEQ ID NOs. 34 to 54 (such as SEQ ID NO. 34, SEQ ID NO. 35, SEQ ID NO. 36, SEQ ID NO. 37, SEQ ID NO. 38, SEQ ID NO. 39, SEQ ID NO. 40, SEQ ID NO. 41, SEQ ID NO. 42, SEQ ID NO. 43, SEQ ID NO. 44, SEQ ID NO. 45, SEQ ID NO. 46, SEQ ID NO. 47, SEQ ID NO. 48, SEQ ID NO. 49, SEQ ID NO. 50, SEQ ID NO. 51, SEQ ID NO. 52, SEQ ID NO. 53, SEQ ID NO. 54, SEQ ID NOs. 34 to 35, SEQ ID NOs. 34 to 36, SEQ ID NOs. 34 to 37, SEQ ID NOs. 34 to 38, SEQ ID NOs. 34 to 39, SEQ ID NOs. 34 to 40, SEQ ID NOs. 34 to 41, SEQ ID NOs. 34 to 42, SEQ ID NOs. 34 to 43, SEQ ID NOs. 34 to 44, SEQ ID NOs. 34 to 45, SEQ ID NOs. 34 to 46, SEQ ID NOs. 34 to 47, SEQ ID NOs. 34 to 48, SEQ ID NOs. 34 to 49, SEQ ID NOs. 34 to 50, SEQ ID NOs. 34 to 51, SEQ ID NOs. 34 to 52, SEQ ID NOs. 34 to 53, and / or SEQ ID NOs. 34 to 54), preferably an amino acid sequence having at least 70% sequence identity with the amino acid sequence of SEQ ID NO. 34.Specifically, the at least one trichothecene is 3-keto-deoxynivalenol, and the at least one polypeptide has at least 70% sequence identity with an amino acid sequence selected from SEQ ID NO. 34 to SEQ ID NO. 54, preferably with the amino acid sequence of SEQ ID NO. 34. Such a polypeptide is particularly effective in converting 3-keto-DON to 3-epi-DON.

[0016] In a preferred embodiment of the method according to the invention, the first trichothecene is deoxynivalenol, and the first reaction mixture is formed by contacting a nutrient composition comprising deoxynivalenol with at least one polypeptide, said at least one polypeptide comprising an amino acid sequence selected from SEQ ID NO.1 to SEQ ID NO.33 (such as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9, SEQ ID NO.10, SEQ ID NO.11, SEQ ID NO.12, SEQ ID NO.13, SEQ ID NO.14, SEQ ID NO.15, SEQ ID NO.16, SEQ ID NO.17, SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.21, SEQ ID NO.22, SEQ ID NO.23, SEQ ID NO.24, SEQ ID NO.25, SEQ ID NO.26, SEQ ID NO.27, SEQ ID NO.28, SEQ ID NO.29, SEQ ID NO.30, SEQ ID NO.31, SEQ ID NO.32, SEQ ID NO.33, SEQ ID NO.1 to SEQ ID NO.2, SEQ ID NO.1 to SEQ ID NO.3, SEQ ID NO.1 to SEQ ID NO.4, SEQ ID NO.1 to SEQ ID NO.5, SEQ ID NO.1 to SEQ ID NO.6, SEQ ID NO.1 to SEQ ID NO.7, SEQ ID NO.1 to SEQ ID NO.8, SEQ ID NO.1 to SEQ ID NO.9, SEQ ID NO.1 to SEQ ID NO.10, SEQ ID NO.1 to SEQ ID NO.11, SEQ ID NO.1 to SEQ ID NO.12, SEQ ID NO.1 to SEQ ID NO.13, SEQ ID NO.1 to SEQ ID NO.14, SEQ ID NO.1 to SEQ ID NO.15, SEQ ID NO.1 to SEQ ID NO.16, SEQ ID NO.1 to SEQ ID NO.17, SEQ ID NO.1 to SEQ ID NO.18, SEQ ID NO.1 to SEQ ID NO.19, SEQ ID NO.1 to SEQ ID NO.20. The amino acid sequence of SEQ ID NO.1 to SEQ ID NO.21, SEQ ID NO.1 to SEQ ID NO.22, SEQ ID NO.1 to SEQ ID NO.23, SEQ ID NO.1 to SEQ ID NO.24, SEQ ID NO.1 to SEQ ID NO.25, SEQ ID NO.1 to SEQ ID NO.26, SEQ ID NO.1 to SEQ ID NO.27, SEQ ID NO.1 to SEQ ID NO.28, SEQ ID NO.1 to SEQ ID NO.29, SEQ ID NO.1 to SEQ ID NO.30, SEQ ID NO.1 to SEQ ID NO.31, SEQ ID NO.1 to SEQ ID NO.32, and / or SEQ ID NO.1 to SEQ ID NO.33), preferably an amino acid sequence having at least 70% sequence identity with the amino acid sequence of SEQ ID NO.1; and the second trichothene is 3-keto-deoxynivalenol, and the second reaction mixture is formed by contacting a nutrient composition containing 3-keto-deoxynivalenol with at least one polypeptide, said at least one polypeptide comprising an amino acid sequence selected from SEQ ID NO.34 to SEQ ID NO.54 (such as SEQ ID NO.34, SEQ ID NO.35, SEQ ID NO.36, SEQ ID NO.37, SEQ ID NO.38, SEQ ID NO.39, SEQ ID NO.40, SEQ ID NO.41, SEQ ID NO.42, SEQ ID NO.43, SEQ ID NO.44, SEQ ID NO.45, SEQ ID NO.46, SEQ ID NO.47, SEQ ID NO.48, SEQ ID NO.49, SEQ ID NO.50, SEQ ID NO.51, SEQ ID NO.52, SEQ ID NO.53, SEQ ID NO.54, SEQ ID NO.34 to SEQ ID NO.35, SEQ ID NO.34 to SEQ ID NO.36, SEQ ID NO.34 to SEQ ID NO.37, SEQ ID NO.34 to SEQ ID NO.38, SEQ ID NO.34 to SEQ ID NO.39, SEQ ID NO.34 to SEQ ID NO.40, SEQ ID NO.34 to SEQ ID NO.41, SEQ ID NO.34 to SEQ ID NO.42, SEQ ID NO.34 to SEQ ID NO.43, SEQ ID NO.The amino acid sequences of SEQ ID NO. 34 to SEQ ID NO. 44, SEQ ID NO. 34 to SEQ ID NO. 45, SEQ ID NO. 34 to SEQ ID NO. 46, SEQ ID NO. 34 to SEQ ID NO. 47, SEQ ID NO. 34 to SEQ ID NO. 48, SEQ ID NO. 34 to SEQ ID NO. 49, SEQ ID NO. 34 to SEQ ID NO. 50, SEQ ID NO. 34 to SEQ ID NO. 51, SEQ ID NO. 34 to SEQ ID NO. 52, SEQ ID NO. 34 to SEQ ID NO. 53, and / or SEQ ID NO. 34 to SEQ ID NO. 54), preferably an amino acid sequence having at least 70% sequence identity with the amino acid sequence of SEQ ID NO. 34. In a specific embodiment, the first trichothene is deoxynivalenol, and the first reaction mixture is formed by contacting a nutrient composition containing deoxynivalenol with at least one polypeptide, the at least one polypeptide being selected from SEQ ID NO. 1 to SEQ ID NO. 33 (such as SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6, SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9, SEQ ID NO. 10, SEQ ID NO. 11, SEQ ID NO. 12, SEQ ID NO. 13, SEQ ID NO. 14, SEQ ID NO. 15, SEQ ID NO. 16, SEQ ID NO. 17, SEQ ID NO. 18, SEQ ID NO. 19, SEQ ID NO. 20, SEQ ID NO. 21, SEQ ID NO. 22, SEQ ID NO. 23, SEQ ID NO. 24, SEQ ID NO. 25, SEQ ID NO. 26, SEQ ID NO. 27, SEQ ID NO. 28, SEQ ID NO. 29, SEQ ID NO. 30, SEQ ID NO. 31, SEQ ID NO. 32, SEQ ID NO. 33, SEQ ID NO. 1 to SEQ ID NO. 2, SEQ ID NO. 1 to SEQ ID NO. 3, SEQ ID NO. 1 to SEQ ID NO. 4, SEQ ID NO. 1 to SEQ ID NO. 5, SEQ ID NO. 1 to SEQ ID NO. 6, SEQ ID NO. 1 to SEQ ID NO. 7, SEQ ID NO.The amino acid sequence of 1 to SEQ ID NO.8, SEQ ID NO.1 to SEQ ID NO.9, SEQ ID NO.1 to SEQ ID NO.10, SEQ ID NO.1 to SEQ ID NO.11, SEQ ID NO.1 to SEQ ID NO.12, SEQ ID NO.1 to SEQ ID NO.13, SEQ ID NO.1 to SEQ ID NO.14, SEQ ID NO.1 to SEQ ID NO.15, SEQ ID NO.1 to SEQ ID NO.16, SEQ ID NO.1 to SEQ ID NO.17, SEQ ID NO.1 to SEQ ID NO.18, SEQ ID NO.1 to SEQ ID NO.19, SEQ ID NO.1 to SEQ ID NO.20, SEQ ID NO.1 to SEQ ID NO.21, SEQ ID NO.1 to SEQ ID NO.22, SEQ ID NO.1 to SEQ ID NO.23, SEQ ID NO.1 to SEQ ID NO.24, SEQ ID NO.1 to SEQ ID NO.25, SEQ ID NO.1 to SEQ ID NO.26, SEQ ID NO.1 to SEQ ID NO.27, SEQ ID NO.1 to SEQ ID NO.28, SEQ ID NO.1 to SEQ ID NO.29, SEQ ID NO.1 to SEQ ID NO.30, SEQ ID NO.1 to SEQ ID NO.31, SEQ ID NO.1 to SEQ ID NO.32, and / or SEQ ID NO.1 to SEQ ID NO.33), preferably having at least 70% sequence identity with the amino acid sequence of SEQ ID NO.1; and the second trichothene is 3-keto-deoxynivalenol, and the second reaction mixture is formed by contacting a nutrient composition containing 3-keto-deoxynivalenol with at least one polypeptide, said at least one polypeptide being selected from SEQ ID NO.34 to SEQ ID NO.54 (such as SEQ ID NO.34, SEQ ID NO.35, SEQ ID NO.36, SEQ ID NO.37, SEQ ID NO.38, SEQ ID NO.39, SEQ ID NO.40, SEQ ID NO.41, SEQ ID NO.42, SEQ ID NO.43, SEQ ID NO.44, SEQ ID NO.45, SEQ ID NO.46, SEQ ID NO.47, SEQ ID NO.48, SEQ ID NO.49, SEQ ID NO.the amino acid sequences of SEQ ID NO.50, SEQ ID NO.51, SEQ ID NO.52, SEQ ID NO.53, SEQ ID NO.54, SEQ ID NO.34 to SEQ ID NO.35, SEQ ID NO.34 to SEQ ID NO.36, SEQ ID NO.34 to SEQ ID NO.37, SEQ ID NO.34 to SEQ ID NO.38, SEQ ID NO.34 to SEQ ID NO.39, SEQ ID NO.34 to SEQ ID NO.40, SEQ ID NO.34 to SEQ ID NO.41, SEQ ID NO.34 to SEQ ID NO.42, SEQ ID NO.34 to SEQ ID NO.43, SEQ ID NO.34 to SEQ ID NO.44, SEQ ID NO.34 to SEQ ID NO.45, SEQ ID NO.34 to SEQ ID NO.46, SEQ ID NO.34 to SEQ ID NO.47, SEQ ID NO.34 to SEQ ID NO.48, SEQ ID NO.34 to SEQ ID NO.49, SEQ ID NO.34 to SEQ ID NO.50, SEQ ID NO.34 to SEQ ID NO.51, SEQ ID NO.34 to SEQ ID NO.52, SEQ ID NO.34 to SEQ ID NO.53, and / or SEQ ID NO.34 to SEQ ID NO.54), preferably having at least 70% sequence identity with the amino acid sequence of SEQ ID NO.34.

[0017] In a specific embodiment, the solution includes causing at least one to comprise a sequence selected from SEQ ID NO.1 to SEQ ID NO.33 (such as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9, SEQ ID NO.10, SEQ ID NO.11, SEQ ID NO.12, SEQ ID NO.13, SEQ ID NO.14, SEQ ID NO.15, SEQ ID NO.16, SEQ ID NO.17, SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.21, SEQ ID NO.22, SEQ ID NO.23, SEQ ID NO.24, SEQ ID NO.25, SEQ ID NO.26, SEQ ID NO.27, SEQ ID NO.28, SEQ ID NO.29, SEQ ID NO.30, SEQ ID NO.31, SEQ ID NO.32, SEQ ID NO.33, SEQ ID NO.1 to SEQ ID NO.2, SEQ ID NO.1 to SEQ ID NO.3, SEQ ID NO.1 to SEQ ID NO.4, SEQ ID NO.1 to SEQ ID NO.5, SEQ ID NO.1 to SEQ ID NO.6, SEQ ID NO.1 to SEQ ID NO.7, SEQ ID NO.1 to SEQ ID NO.8, SEQ ID NO.1 to SEQ ID NO.9, SEQ ID NO.1 to SEQ ID NO.10, SEQ ID NO.1 to SEQ ID NO.11, SEQ ID NO.1 to SEQ ID NO.12, SEQ ID NO.1 to SEQ ID NO.13, SEQ ID NO.1 to SEQ ID NO.14, SEQ ID NO.1 to SEQ ID NO.15, SEQ ID NO.1 to SEQ ID NO.16, SEQ ID NO.1 to SEQ ID NO.17, SEQ ID NO.1 to SEQ ID NO.18, SEQ ID NO.1 to SEQ ID NO.19, SEQ ID NO.1 to SEQ ID NO.20, SEQ ID NO.1 to SEQ ID NO.21, SEQ ID NO.1 to SEQ ID NO.22, SEQ ID NO.1 to SEQ ID NO.23, an amino acid sequence of SEQ ID NO.1 to SEQ ID NO.24, SEQ ID NO.1 to SEQ ID NO.25, SEQ ID NO.1 to SEQ ID NO.26, SEQ ID NO.1 to SEQ ID NO.27, SEQ ID NO.1 to SEQ ID NO.28, SEQ ID NO.1 to SEQ ID NO.29, SEQ ID NO.1 to SEQ ID NO.30, SEQ ID NO.1 to SEQ ID NO.31, SEQ ID NO.1 to SEQ ID NO.32, and / or SEQ ID NO.1 to SEQ ID NO.33), preferably a polypeptide having an amino acid sequence with at least 70% sequence identity to the amino acid sequence of SEQ ID NO.1, and a nutritional composition containing deoxynivalenol and with NAD. + Contact, so as to form 3-keto-deoxynivalenol from the deoxynivalenol and from the NAD +The first step of forming NADH; and contacting the 3-keto-deoxynivalenol formed in the first step with at least one polypeptide comprising an amino acid sequence selected from SEQ ID NOs. 34 to 54 (such as SEQ ID NO. 34, SEQ ID NO. 35, SEQ ID NO. 36, SEQ ID NO. 37, SEQ ID NO. 38, SEQ ID NO. 39, SEQ ID NO. 40, SEQ ID NO. 41, SEQ ID NO. 42, SEQ ID NO. 43, SEQ ID NO. 44, SEQ ID NO. 45, SEQ ID NO. 46, SEQ ID NO. 47, SEQ ID NO. 48, SEQ ID NO. 49, SEQ ID NO. 50, SEQ ID NO. 51, SEQ ID NO. 52, SEQ ID NO. 53, SEQ ID NO. 54, SEQ ID NOs. 34 to 35, SEQ ID NOs. 34 to 36, SEQ ID NOs. 34 to 37, SEQ ID NOs. 34 to 38, SEQ ID NOs. 34 to 39, SEQ ID NOs. 34 to 40, SEQ ID NOs. 34 to 41, SEQ ID NOs. 34 to 42, SEQ ID NOs. 34 to 43, SEQ ID NOs. 34 to 44, SEQ ID NOs. 34 to 45, SEQ ID NOs. 34 to 46, SEQ ID NOs. 34 to 47, SEQ ID NOs. 34 to 48, SEQ ID NOs. 34 to 49, SEQ ID NOs. 34 to 50, SEQ ID NOs. 34 to 51, SEQ ID NOs. 34 to 52, SEQ ID NOs. 34 to 53, and / or SEQ ID NOs. 34 to 54), preferably an amino acid sequence having at least 70% sequence identity with the amino acid sequence of SEQ ID NO. 34, and contacting with NADH, preferably with the NADH formed in the first step; thereby forming 3-epi-deoxynivalenol from 3-keto-deoxynivalenol and forming NAD from NADH +The second step. In other words, in the specific embodiment, the present invention relates to a method for modifying DON to form 3-epi-DON, the method comprising: 1) by causing at least one to comprise a sequence selected from SEQ ID NO.1 to SEQ ID NO.33 (such as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9, SEQ ID NO.10, SEQ ID NO.11, SEQ ID NO.12, SEQ ID NO.13, SEQ ID NO.14, SEQ ID NO.15, SEQ ID NO.16, SEQ ID NO.17, SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.21, SEQ ID NO.22, SEQ ID NO.23, SEQ ID NO.24, SEQ ID NO.25, SEQ ID NO.26, SEQ ID NO.27, SEQ ID NO.28, SEQ ID NO.29, SEQ ID NO.30, SEQ ID NO.31, SEQ ID NO.32, SEQ ID NO.33, SEQ ID NO.1 to SEQ ID NO.2, SEQ ID NO.1 to SEQ ID NO.3, SEQ ID NO.1 to SEQ ID NO.4, SEQ ID NO.1 to SEQ ID NO.5, SEQ ID NO.1 to SEQ ID NO.6, SEQ ID NO.1 to SEQ ID NO.7, SEQ ID NO.1 to SEQ ID NO.8, SEQ ID NO.1 to SEQ ID NO.9, SEQ ID NO.1 to SEQ ID NO.10, SEQ ID NO.1 to SEQ ID NO.11, SEQ ID NO.1 to SEQ ID NO.12, SEQ ID NO.1 to SEQ ID NO.13, SEQ ID NO.1 to SEQ ID NO.14, SEQ ID NO.1 to SEQ ID NO.15, SEQ ID NO.1 to SEQ ID NO.16, SEQ ID NO.1 to SEQ ID NO.17, SEQ ID NO.1 to SEQ ID NO.18, SEQ ID NO.1 to SEQ ID NO.19, SEQ ID NO.1 to SEQ ID NO.20, SEQ ID NO.1 to SEQ ID NO.21. A polypeptide having an amino acid sequence of SEQ ID NO.1 to SEQ ID NO.22, SEQ ID NO.1 to SEQ ID NO.23, SEQ ID NO.1 to SEQ ID NO.24, SEQ ID NO.1 to SEQ ID NO.25, SEQ ID NO.1 to SEQ ID NO.26, SEQ ID NO.1 to SEQ ID NO.27, SEQ ID NO.1 to SEQ ID NO.28, SEQ ID NO.1 to SEQ ID NO.29, SEQ ID NO.1 to SEQ ID NO.30, SEQ ID NO.1 to SEQ ID NO.31, SEQ ID NO.1 to SEQ ID NO.32, and / or SEQ ID NO.1 to SEQ ID NO.33), preferably a polypeptide having an amino acid sequence with at least 70% sequence identity to the amino acid sequence of SEQ ID NO.1, and a nutritional composition containing deoxynivalenol and NAD. +Contact to form 3-keto-DON and NADH; and 2) by contacting at least one polypeptide comprising an amino acid sequence selected from SEQ ID NO. 34 to SEQ ID NO. 54 (such as SEQ ID NO. 34, SEQ ID NO. 35, SEQ ID NO. 36, SEQ ID NO. 37, SEQ ID NO. 38, SEQ ID NO. 39, SEQ ID NO. 40, SEQ ID NO. 41, SEQ ID NO. 42, SEQ ID NO. 43, SEQ ID NO. 44, SEQ ID NO. 45, SEQ ID NO. 46, SEQ ID NO. 47, SEQ ID NO. 48, SEQ ID NO. 49, SEQ ID NO. 50, SEQ ID NO. 51, SEQ ID NO. 52, SEQ ID NO. 53, SEQ ID NO. 54, SEQ ID NO. 34 to SEQ ID NO. 35, SEQ ID NO. 34 to SEQ ID NO. 36, SEQ ID NO. 34 to SEQ ID NO. 37, SEQ ID NO. 34 to SEQ ID NO. 38, SEQ ID NO. 34 to SEQ ID NO. 39, SEQ ID NO. 34 to SEQ ID NO. 40, SEQ ID NO. 34 to SEQ ID NO. 41, SEQ ID NO. 34 to SEQ ID NO. 42, SEQ ID NO. 34 to SEQ ID NO. 43, SEQ ID NO. 34 to SEQ ID NO. 44, SEQ ID NO. 34 to SEQ ID NO. 45, SEQ ID NO. 34 to SEQ ID NO. 46, SEQ ID NO. 34 to SEQ ID NO. 47, SEQ ID NO. 34 to SEQ ID NO. 48, SEQ ID NO. 34 to SEQ ID NO. 49, SEQ ID NO. 34 to SEQ ID NO. 50, SEQ ID NO. 34 to SEQ ID NO. 51, SEQ ID NO. 34 to SEQ ID NO. 52, SEQ ID NO. 34 to SEQ ID NO. 53, and / or SEQ ID NO. 34 to SEQ ID NO. 54), preferably an amino acid sequence having at least 70% sequence identity with the amino acid sequence of SEQ ID NO. 34, with the 3-keto-DON formed in step 1) and contacting with NADH, preferably contacting with the NADH formed in step 1), to form 3-epi-DON and NAD +。In this way, DON is first modified to 3-keto-DON while NAD + is reduced to NADH, and in a second step, 3-keto-DON is reduced to form 3-epi-DON while NADH is recycled back to its oxidized form NAD +. Thus, the preferred cofactor NAD(H) can be recycled and reused in further cycles of the method. Advantageously, such a method requires only at least one comprising a sequence selected from SEQ ID NO.1 to SEQ ID NO.33 (such as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9, SEQ ID NO.10, SEQ ID NO.11, SEQ ID NO.12, SEQ ID NO.13, SEQ ID NO.14, SEQ ID NO.15, SEQ ID NO.16, SEQ ID NO.17, SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.21, SEQ ID NO.22, SEQ ID NO.23, SEQ ID NO.24, SEQ ID NO.25, SEQ ID NO.26, SEQ ID NO.27, SEQ ID NO.28, SEQ ID NO.29, SEQ ID NO.30, SEQ ID NO.31, SEQ ID NO.32, SEQ ID NO.33, SEQ ID NO.1 to SEQ ID NO.2, SEQ ID NO.1 to SEQ ID NO.3, SEQ ID NO.1 to SEQ ID NO.4, SEQ ID NO.1 to SEQ ID NO.5, SEQ ID NO.1 to SEQ ID NO.6, SEQ ID NO.1 to SEQ ID NO.7, SEQ ID NO.1 to SEQ ID NO.8, SEQ ID NO.1 to SEQ ID NO.9, SEQ ID NO.1 to SEQ ID NO.10, SEQ ID NO.1 to SEQ ID NO.11, SEQ ID NO.1 to SEQ ID NO.12, SEQ ID NO.1 to SEQ ID NO.13, SEQ ID NO.1 to SEQ ID NO.14, SEQ ID NO.1 to SEQ ID NO.15, SEQ ID NO.1 to SEQ ID NO.16, SEQ ID NO.1 to SEQ ID NO.17, SEQ ID NO.1 to SEQ ID NO.18, SEQ ID NO.1 to SEQ ID NO.19, SEQ ID NO.1 to SEQ ID NO.20, SEQ ID NO.1 to SEQ ID NO.21, SEQ ID NO.The amino acid sequence of 1 to SEQ ID NO.22, SEQ ID NO.1 to SEQ ID NO.23, SEQ ID NO.1 to SEQ ID NO.24, SEQ ID NO.1 to SEQ ID NO.25, SEQ ID NO.1 to SEQ ID NO.26, SEQ ID NO.1 to SEQ ID NO.27, SEQ ID NO.1 to SEQ ID NO.28, SEQ ID NO.1 to SEQ ID NO.29, SEQ ID NO.1 to SEQ ID NO.30, SEQ ID NO.1 to SEQ ID NO.31, SEQ ID NO.1 to SEQ ID NO.32, and / or SEQ ID NO.1 to SEQ ID NO.33); preferably a polypeptide having an amino acid sequence with at least 70% sequence identity to the amino acid sequence of SEQ ID NO.1; at least one additional polypeptide comprising an amino acid sequence selected from SEQ ID NO.34 to SEQ ID NO.54 (such as SEQ ID NO.34, SEQ ID NO.35, SEQ ID NO.36, SEQ ID NO.37, SEQ ID NO.38, SEQ ID NO.39, SEQ ID NO.40, SEQ ID NO.41, SEQ ID NO.42, SEQ ID NO.43, SEQ ID NO.44, SEQ ID NO.45, SEQ ID NO.46, SEQ ID NO.47, SEQ ID NO.48, SEQ ID NO.49, SEQ ID NO.50, SEQ ID NO.51, SEQ ID NO.52, SEQ ID NO.53, SEQ ID NO.54, SEQ ID NO.34 to SEQ ID NO.35, SEQ ID NO.34 to SEQ ID NO.36, SEQ ID NO.34 to SEQ ID NO.37, SEQ ID NO.34 to SEQ ID NO.38, SEQ ID NO.34 to SEQ ID NO.39, SEQ ID NO.34 to SEQ ID NO.40, SEQ ID NO.34 to SEQ ID NO.41, SEQ ID NO.34 to SEQ ID NO.42, SEQ ID NO.34 to SEQ ID NO.43, SEQ ID NO.34 to SEQ ID NO.44, SEQ ID NO.34 to SEQ ID NO.45, SEQ ID NO.34 to SEQ ID NO.46, SEQ ID NO.34 to SEQ ID NO.47, SEQ ID NO.an amino acid sequence selected from SEQ ID NO. 34 to SEQ ID NO. 48, SEQ ID NO. 34 to SEQ ID NO. 49, SEQ ID NO. 34 to SEQ ID NO. 50, SEQ ID NO. 34 to SEQ ID NO. 51, SEQ ID NO. 34 to SEQ ID NO. 52, SEQ ID NO. 34 to SEQ ID NO. 53, and / or SEQ ID NO. 34 to SEQ ID NO. 54), preferably a polypeptide having an amino acid sequence with at least 70% sequence identity to the amino acid sequence of SEQ ID NO. 34; and an NAD(H) cofactor to repeatedly convert DON to 3-epi-DON.

[0018] In some embodiments, at least one polypeptide consisting of or comprising an amino acid sequence selected from the amino acid sequences of SEQ ID NO. 1 to SEQ ID NO. 33, preferably having an amino acid sequence with at least 70% sequence identity to the amino acid sequence of SEQ ID NO. 1, and at least one polypeptide consisting of or comprising an amino acid sequence selected from the amino acid sequences of SEQ ID NO. 34 to SEQ ID NO. 54, preferably having an amino acid sequence with at least 70% sequence identity to the amino acid sequence of SEQ ID NO. 34 are each added to the nutritional composition to achieve a final concentration of at least 0.1 mM, preferably at least 1 mM (such as 1.0 mM, 1.1 mM, 1.2 mM, 1.3 mM, 1.4 mM or 1.5 mM).

[0019] In another aspect, the present invention relates to a composition for modifying at least one trichothecene (e.g., detoxifying at least one trichothecene), such as an additive for food and / or feed; feed or food, the composition comprising at least one polypeptide having an amino acid sequence with at least 70% sequence identity to an amino acid sequence selected from SEQ ID NO.1 to SEQ ID NO.54 (e.g., SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9, SEQ ID NO.10, SEQ ID NO.11, SEQ ID NO.12, SEQ ID NO.13, SEQ ID NO.14, SEQ ID NO.15, SEQ ID NO.16, SEQ ID NO.17, SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.21, SEQ ID NO.22, SEQ ID NO.23, SEQ ID NO.24, SEQ ID NO.25, SEQ ID NO.26, SEQ ID NO.27, SEQ ID NO.28, SEQ ID NO.29, SEQ ID NO.30, SEQ ID NO.31, SEQ ID NO.32, SEQ ID NO.33, SEQ ID NO.34, SEQ ID NO.35, SEQ ID NO.36, SEQ ID NO.37, SEQ ID NO.38, SEQ ID NO.39, SEQ ID NO.40, SEQ ID NO.41, SEQ ID NO.42, SEQ ID NO.43, SEQ ID NO.44, SEQ ID NO.45, SEQ ID NO.46, SEQ ID NO.47, SEQ ID NO.48, SEQ ID NO.49, SEQ ID NO.50, SEQ ID NO.51, SEQ ID NO.52, SEQ ID NO.53, and / or SEQ ID NO.54), preferably selected from SEQ ID NO.1 and SEQ ID NO.34. Such compositions are capable of detoxifying trichothecenes, such as DON and / or 3-keto-DON.

[0020] In some embodiments of the present invention, the composition is substantially free of cofactors other than NAD(H) and / or NADP(H), and preferably the composition is substantially free of cofactors other than NAD(H). In a specific embodiment, the composition is free of pyrroloquinoline quinone (PQQ; CAS No. 72909-34-3).

[0021] The composition according to the present invention can be, for example, a food additive; a food additive; a forage additive; a feed additive; a nutritional supplement; an intermediate thereof; and / or a mixture thereof. Such compositions may contain additional components, such as prebiotics and / or probiotics. For purposes of clarification only, forage or feed can, for example, comprise or consist of corn, hay, straw bedding, soybeans, or products obtained therefrom. Again, forage or feed can comprise or consist of expanded feed products (such as pellets). Additives for food, foodstuffs, forage, or feed are often used to improve or enhance the properties of the food, forage, or feed. For example, such additives can be added to improve sensory properties, such as to improve the taste, odor, appearance, or color of the food, foodstuff, forage, or feed. Again, additives can be added to improve palatability, nutrient utilization, or to add probiotic microorganisms to the food, foodstuff, forage, or feed, or to add or enhance the prebiotic activity of the food, foodstuff, forage, or feed. Again, such additives can be added to counteract potential adverse effects of the food, foodstuff, forage, or feed, such as to remove or reduce one or more undesired components contained in the food, foodstuff, forage, or feed.

[0022] In some embodiments, the composition further comprises a cofactor selected from NAD(H) and NADP(H). Thus, the composition is not affected by varying exogenous NAD(H) / NADP(H) cofactor concentrations.

[0023] In a preferred embodiment, the composition comprises at least one comprising a sequence selected from SEQ ID NO.1 to SEQ ID NO.54 (e.g., SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9, SEQ ID NO.10, SEQ ID NO.11, SEQ ID NO.12, SEQ ID NO.13, SEQ ID NO.14, SEQ ID NO.15, SEQ ID NO.16, SEQ ID NO.17, SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.21, SEQ ID NO.22, SEQ ID NO.23, SEQ ID NO.24, SEQ ID NO.25, SEQ ID NO.26, SEQ ID NO.27, SEQ ID NO.28, SEQ ID NO.29, SEQ ID NO.30, SEQ ID NO.31, SEQ ID NO.32, SEQ ID NO.33, SEQ ID NO.34, SEQ ID NO.35, SEQ ID NO.36, SEQ ID NO.37, SEQ ID NO.38, SEQ ID NO.39, SEQ ID NO.40, SEQ ID NO.41, SEQ ID NO.42, SEQ ID NO.43, SEQ ID NO.44, SEQ ID NO.45, SEQ ID NO.46, SEQ ID NO.47, SEQ ID NO.48, SEQ ID NO.49, SEQ ID NO.50, SEQ ID NO.51, SEQ ID NO.52, SEQ ID NO.53, SEQ ID NO.54, SEQ ID NO.1 to SEQ ID NO.54, SEQ ID NO.1 to SEQ ID NO.2, SEQ ID NO.1 to SEQ ID NO.3, SEQ ID NO.1 to SEQ ID NO.4, SEQ ID NO.1 to SEQ ID NO.5, SEQ ID NO.1 to SEQ ID NO.6, SEQ ID NO.1 to SEQ ID NO.7, SEQ ID NO.1 to SEQ ID NO.8, SEQ ID NO.1 to SEQ ID NO.9, SEQ ID NO.1 to SEQ ID NO.10, SEQ ID NO.1 to SEQ ID NO.11. SEQ ID NO.1 to SEQ ID NO.12, SEQ ID NO.1 to SEQ ID NO.13, SEQ ID NO.1 to SEQ ID NO.14, SEQ ID NO.1 to SEQ ID NO.15, SEQ ID NO.1 to SEQ ID NO.16, SEQ ID NO.1 to SEQ ID NO.17, SEQ ID NO.1 to SEQ ID NO.18, SEQ ID NO.1 to SEQ ID NO.19, SEQ ID NO.1 to SEQ ID NO.20, SEQ ID NO.1 to SEQ ID NO.21, SEQ ID NO.1 to SEQ ID NO.22, SEQ ID NO.1 to SEQ ID NO.23, SEQ ID NO.1 to SEQ ID NO.24, SEQ ID NO.1 to SEQ ID NO.25, SEQ ID NO.1 to SEQ ID NO.26, SEQ ID NO.1 to SEQ ID NO.27, SEQ ID NO.1 to SEQ ID NO.28, SEQ ID NO.1 to SEQ ID NO.29, SEQ ID NO.1 to SEQ ID NO.30, SEQ ID NO.1 to SEQ ID NO.31, SEQ ID NO.1 to SEQ ID NO.32, SEQ ID NO.1 to SEQ ID NO.33, SEQ ID NO.34 to SEQ ID NO.35, SEQ ID NO.34 to SEQ ID NO.36, SEQ ID NO.34 to SEQ ID NO.37, SEQ ID NO.34 to SEQ ID NO.38, SEQ ID NO.34 to SEQ ID NO.39, SEQ ID NO.34 to SEQ ID NO.40, SEQ ID NO.34 to SEQ ID NO.41, SEQ ID NO.34 to SEQ ID NO.42, SEQ ID NO.34 to SEQ ID NO.43, SEQ ID NO.34 to SEQ ID NO.44, SEQ ID NO.34 to SEQ ID NO.45, SEQ ID NO.34 to SEQ ID NO.46, SEQ ID NO.34 to SEQ ID NO.47, SEQ ID NO.34 to SEQ ID NO.48, SEQ ID NO.34 to SEQ ID NO.49, SEQ ID NO.34 to SEQ ID NO.50, SEQ ID NO.34 to SEQ ID NO.51, SEQ ID NO.a polypeptide having an amino acid sequence of at least 70% sequence identity to the amino acid sequences of SEQ ID NO. 34 to SEQ ID NO. 52, SEQ ID NO. 34 to SEQ ID NO. 53, and / or SEQ ID NO. 34 to SEQ ID NO. 54), preferably selected from the amino acid sequences having at least 70% sequence identity to SEQ ID NO. 1 and SEQ ID NO. 34; and NAD(H). An advantage of NAD(H) is that it is less susceptible to acids than NADP(H) and is a more stable molecule. In a specific embodiment, the composition comprises at least one polypeptide having an amino acid sequence of at least 70% sequence identity to the amino acid sequences selected from SEQ ID NO. 1 to SEQ ID NO. 54, preferably selected from SEQ ID NO. 1 and SEQ ID NO. 34; and NAD(H).

[0024] In some embodiments, the composition according to the present invention comprises at least two polypeptides, wherein at least the first polypeptide comprises an amino acid sequence selected from SEQ ID NO.1 to SEQ ID NO.33 (such as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9, SEQ ID NO.10, SEQ ID NO.11, SEQ ID NO.12, SEQ ID NO.13, SEQ ID NO.14, SEQ ID NO.15, SEQ ID NO.16, SEQ ID NO.17, SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.21, SEQ ID NO.22, SEQ ID NO.23, SEQ ID NO.24, SEQ ID NO.25, SEQ ID NO.26, SEQ ID NO.27, SEQ ID NO.28, SEQ ID NO.29, SEQ ID NO.30, SEQ ID NO.31, SEQ ID NO.32, SEQ ID NO.33, SEQ ID NO.1 to SEQ ID NO.2, SEQ ID NO.1 to SEQ ID NO.3, SEQ ID NO.1 to SEQ ID NO.4, SEQ ID NO.1 to SEQ ID NO.5, SEQ ID NO.1 to SEQ ID NO.6, SEQ ID NO.1 to SEQ ID NO.7, SEQ ID NO.1 to SEQ ID NO.8, SEQ ID NO.1 to SEQ ID NO.9, SEQ ID NO.1 to SEQ ID NO.10, SEQ ID NO.1 to SEQ ID NO.11, SEQ ID NO.1 to SEQ ID NO.12, SEQ ID NO.1 to SEQ ID NO.13, SEQ ID NO.1 to SEQ ID NO.14, SEQ ID NO.1 to SEQ ID NO.15, SEQ ID NO.1 to SEQ ID NO.16, SEQ ID NO.1 to SEQ ID NO.17, SEQ ID NO.1 to SEQ ID NO.18, SEQ ID NO.1 to SEQ ID NO.19, SEQ ID NO.1 to SEQ ID NO.20, SEQ ID NO.1 to SEQ ID NO.21, SEQ ID NO.1 to SEQ ID NO.22. At least one amino acid sequence of SEQ ID NO.1 to SEQ ID NO.23, SEQ ID NO.1 to SEQ ID NO.24, SEQ ID NO.1 to SEQ ID NO.25, SEQ ID NO.1 to SEQ ID NO.26, SEQ ID NO.1 to SEQ ID NO.27, SEQ ID NO.1 to SEQ ID NO.28, SEQ ID NO.1 to SEQ ID NO.29, SEQ ID NO.1 to SEQ ID NO.30, SEQ ID NO.1 to SEQ ID NO.31, SEQ ID NO.1 to SEQ ID NO.32, and / or SEQ ID NO.1 to SEQ ID NO.33); preferably an amino acid sequence having at least 70% sequence identity with the amino acid sequence of SEQ ID NO.1; and at least a second polypeptide comprises an amino acid sequence selected from SEQ ID NO.34 to SEQ ID NO.54 (such as SEQ ID NO.34, SEQ ID NO.35, SEQ ID NO.36, SEQ ID NO.37, SEQ ID NO.38, SEQ ID NO.39, SEQ ID NO.40, SEQ ID NO.41, SEQ ID NO.42, SEQ ID NO.43, SEQ ID NO.44, SEQ ID NO.45, SEQ ID NO.46, SEQ ID NO.47, SEQ ID NO.48, SEQ ID NO.49, SEQ ID NO.50, SEQ ID NO.51, SEQ ID NO.52, SEQ ID NO.53, SEQ ID NO.54, SEQ ID NO.34 to SEQ ID NO.35, SEQ ID NO.34 to SEQ ID NO.36, SEQ ID NO.34 to SEQ ID NO.37, SEQ ID NO.34 to SEQ ID NO.38, SEQ ID NO.34 to SEQ ID NO.39, SEQ ID NO.34 to SEQ ID NO.40, SEQ ID NO.34 to SEQ ID NO.41, SEQ ID NO.34 to SEQ ID NO.42, SEQ ID NO.34 to SEQ ID NO.43, SEQ ID NO.34 to SEQ ID NO.44, SEQ ID NO.34 to SEQ ID NO.45, SEQ ID NO.34 to SEQ ID NO.46, SEQ ID NO.34 to SEQ ID NO.47, SEQ ID NO.34 to SEQ ID NO.48. At least one amino acid sequence of SEQ ID NO.34 to SEQ ID NO.49, SEQ ID NO.34 to SEQ ID NO.50, SEQ ID NO.34 to SEQ ID NO.51, SEQ ID NO.34 to SEQ ID NO.52, SEQ ID NO.34 to SEQ ID NO.53, and / or SEQ ID NO.34 to SEQ ID NO.54), preferably an amino acid sequence having at least 70% sequence identity with the amino acid sequence of SEQ ID NO.34. In a specific embodiment, the composition according to the present invention comprises at least two polypeptides, wherein at least the first polypeptide is selected from SEQ ID NO.1 to SEQ ID NO.33 (such as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9, SEQ ID NO.10, SEQ ID NO.11, SEQ ID NO.12, SEQ ID NO.13, SEQ ID NO.14, SEQ ID NO.15, SEQ ID NO.16, SEQ ID NO.17, SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.21, SEQ ID NO.22, SEQ ID NO.23, SEQ ID NO.24, SEQ ID NO.25, SEQ ID NO.26, SEQ ID NO.27, SEQ ID NO.28, SEQ ID NO.29, SEQ ID NO.30, SEQ ID NO.31, SEQ ID NO.32, SEQ ID NO.33, SEQ ID NO.1 to SEQ ID NO.2, SEQ ID NO.1 to SEQ ID NO.3, SEQ ID NO.1 to SEQ ID NO.4, SEQ ID NO.1 to SEQ ID NO.5, SEQ ID NO.1 to SEQ ID NO.6, SEQ ID NO.1 to SEQ ID NO.7, SEQ ID NO.1 to SEQ ID NO.8, SEQ ID NO.1 to SEQ ID NO.9, SEQ ID NO.1 to SEQ ID NO.10, SEQ ID NO.1 to SEQ ID NO.11, SEQ ID NO.1 to SEQ ID NO.12, SEQ ID NO.1 to SEQ ID NO.13, SEQ ID NO.at least one amino acid sequence of 1 to SEQ ID NO.14, SEQ ID NO.1 to SEQ ID NO.15, SEQ ID NO.1 to SEQ ID NO.16, SEQ ID NO.1 to SEQ ID NO.17, SEQ ID NO.1 to SEQ ID NO.18, SEQ ID NO.1 to SEQ ID NO.19, SEQ ID NO.1 to SEQ ID NO.20, SEQ ID NO.1 to SEQ ID NO.21, SEQ ID NO.1 to SEQ ID NO.22, SEQ ID NO.1 to SEQ ID NO.23, SEQ ID NO.1 to SEQ ID NO.24, SEQ ID NO.1 to SEQ ID NO.25, SEQ ID NO.1 to SEQ ID NO.26, SEQ ID NO.1 to SEQ ID NO.27, SEQ ID NO.1 to SEQ ID NO.28, SEQ ID NO.1 to SEQ ID NO.29, SEQ ID NO.1 to SEQ ID NO.30, SEQ ID NO.1 to SEQ ID NO.31, SEQ ID NO.1 to SEQ ID NO.32, and / or SEQ ID NO.1 to SEQ ID NO.33), preferably having at least 70% sequence identity with the amino acid sequence of SEQ ID NO.1; and at least a second polypeptide is selected from SEQ ID NO.34 to SEQ ID NO.54 (such as SEQ ID NO.34, SEQ ID NO.35, SEQ ID NO.36, SEQ ID NO.37, SEQ ID NO.38, SEQ ID NO.39, SEQ ID NO.40, SEQ ID NO.41, SEQ ID NO.42, SEQ ID NO.43, SEQ ID NO.44, SEQ ID NO.45, SEQ ID NO.46, SEQ ID NO.47, SEQ ID NO.48, SEQ ID NO.49, SEQ ID NO.50, SEQ ID NO.51, SEQ ID NO.52, SEQ ID NO.53, SEQ ID NO.54, SEQ ID NO.34 to SEQ ID NO.35, SEQ ID NO.34 to SEQ ID NO.36, SEQ ID NO.34 to SEQ ID NO.37, SEQ ID NO.34 to SEQ ID NO.38, SEQ ID NO.34 to SEQ ID NO.39, SEQ ID NO.34 to SEQ ID NO.At least one amino acid sequence of SEQ ID NO.34 to SEQ ID NO.41, SEQ ID NO.34 to SEQ ID NO.42, SEQ ID NO.34 to SEQ ID NO.43, SEQ ID NO.34 to SEQ ID NO.44, SEQ ID NO.34 to SEQ ID NO.45, SEQ ID NO.34 to SEQ ID NO.46, SEQ ID NO.34 to SEQ ID NO.47, SEQ ID NO.34 to SEQ ID NO.48, SEQ ID NO.34 to SEQ ID NO.49, SEQ ID NO.34 to SEQ ID NO.50, SEQ ID NO.34 to SEQ ID NO.51, SEQ ID NO.34 to SEQ ID NO.52, SEQ ID NO.34 to SEQ ID NO.53, and / or SEQ ID NO.34 to SEQ ID NO.54), preferably having at least 70% sequence identity with the amino acid sequence of SEQ ID NO.34.

[0025] In some embodiments, the compositions of the present invention further comprise at least one carrier. The carrier can be any suitable carrier. The composition can comprise one, two, three or even more carriers. The carrier can be a dietary supplement, a nutritional preparation and / or a drug, such as vitamins, minerals, amino acids, essential fatty acids, fiber, trace elements, antioxidants, plant extracts, herbal extracts and / or essential oils. The carrier can also be a carrier for an enzyme. Carriers for enzymes can be both of inorganic origin and of organic origin. Potential inorganic materials for immobilizing enzymes are silica (sol-gel silica, fumed silica, colloidal silica nanoparticles and silica gel), and oxides such as titanium dioxide, alumina and zirconia. In addition, clay materials such as bentonite, halloysite, kaolinite, montmorillonite, sepiolite and calcium apatite can be carriers. In addition, carbon-based materials such as activated carbon and charcoal can be carriers. Organic enzyme carriers can be biopolymers (such as carbohydrates, proteins, maltodextrin, trehalose, inulin, collagen, cellulose, keratin, carrageenan, chitin, chitosan and alginate) or synthetic polymers (such as polyaniline, polyamide, polystyrene, polyurethane, polypropylene, polyvinyl alcohol and ion exchange resins). Liquid carriers can be, for example, buffer substances and / or polyols such as polyalkylene oxides, polyvinyl alcohol, polyvinyl maleic anhydride copolymer, polystyrene maleic anhydride copolymer, dextran, cellulose, chitosan hydrolyzate, starch, glycogen, sorbitol, agarose and its derivatives, guar gum, amylopectin, inulin, xanthan gum, carrageenan, pectin, alginic acid hydrolyzate, biopolymers, sorbitol, glycerol, cellobiose and monopropylene glycol. The carrier can additionally or alternatively be an edible component, preferably a non-toxic component and / or a component providing texture. In a specific embodiment, the carrier is selected from bentonite, silica and carbohydrates; preferably, the carrier is bentonite.

[0026] In yet another aspect, the present invention relates to the use of at least one polypeptide for modifying trichothecenes, wherein the at least one polypeptide comprises an amino acid sequence having at least 70% sequence identity with an amino acid sequence selected from SEQ ID NO.1 to SEQ ID NO.54, preferably selected from SEQ ID NO.1 and SEQ ID NO.34.

[0027] In some embodiments, the present invention relates to the use of at least one polypeptide for modifying trichothecenes, preferably deoxynivalenol (in particular for detoxifying it), wherein the at least one polypeptide comprises an amino acid sequence selected from SEQ ID NO.1 to SEQ ID NO.33 (such as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9, SEQ ID NO.10, SEQ ID NO.11, SEQ ID NO.12, SEQ ID NO.13, SEQ ID NO.14, SEQ ID NO.15, SEQ ID NO.16, SEQ ID NO.17, SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.21, SEQ ID NO.22, SEQ ID NO.23, SEQ ID NO.24, SEQ ID NO.25, SEQ ID NO.26, SEQ ID NO.27, SEQ ID NO.28, SEQ ID NO.29, SEQ ID NO.30, SEQ ID NO.31, SEQ ID NO.32, SEQ ID NO.33, SEQ ID NO.1 to SEQ ID NO.2, SEQ ID NO.1 to SEQ ID NO.3, SEQ ID NO.1 to SEQ ID NO.4, SEQ ID NO.1 to SEQ ID NO.5, SEQ ID NO.1 to SEQ ID NO.6, SEQ ID NO.1 to SEQ ID NO.7, SEQ ID NO.1 to SEQ ID NO.8, SEQ ID NO.1 to SEQ ID NO.9, SEQ ID NO.1 to SEQ ID NO.10, SEQ ID NO.1 to SEQ ID NO.11, SEQ ID NO.1 to SEQ ID NO.12, SEQ ID NO.1 to SEQ ID NO.13, SEQ ID NO.1 to SEQ ID NO.14, SEQ ID NO.1 to SEQ ID NO.15, SEQ ID NO.1 to SEQ ID NO.16, SEQ ID NO.1 to SEQ ID NO.17, SEQ ID NO.1 to SEQ ID NO.18, SEQ ID NO.1 to SEQ ID NO.19, SEQ ID NO.1 to SEQ ID NO.20, SEQ ID NO.1 to SEQ ID NO.The amino acid sequences of SEQ ID NO.1 to SEQ ID NO.22, SEQ ID NO.1 to SEQ ID NO.23, SEQ ID NO.1 to SEQ ID NO.24, SEQ ID NO.1 to SEQ ID NO.25, SEQ ID NO.1 to SEQ ID NO.26, SEQ ID NO.1 to SEQ ID NO.27, SEQ ID NO.1 to SEQ ID NO.28, SEQ ID NO.1 to SEQ ID NO.29, SEQ ID NO.1 to SEQ ID NO.30, SEQ ID NO.1 to SEQ ID NO.31, SEQ ID NO.1 to SEQ ID NO.32, and / or SEQ ID NO.1 to SEQ ID NO.33), preferably an amino acid sequence having at least 70% sequence identity with the amino acid sequence of SEQ ID NO.1.

[0028] In some embodiments, the present invention relates to the use of at least one polypeptide for modifying trichothecenes, preferably 3-keto-deoxynivalenol (in particular for detoxifying it), wherein the at least one polypeptide comprises an amino acid sequence selected from SEQ ID NOs. 34 to 54 (such as SEQ ID NO. 34, SEQ ID NO. 35, SEQ ID NO. 36, SEQ ID NO. 37, SEQ ID NO. 38, SEQ ID NO. 39, SEQ ID NO. 40, SEQ ID NO. 41, SEQ ID NO. 42, SEQ ID NO. 43, SEQ ID NO. 44, SEQ ID NO. 45, SEQ ID NO. 46, SEQ ID NO. 47, SEQ ID NO. 48, SEQ ID NO. 49, SEQ ID NO. 50, SEQ ID NO. 51, SEQ ID NO. 52, SEQ ID NO. 53, SEQ ID NO. 54, SEQ ID NOs. 34 to 35, SEQ ID NOs. 34 to 36, SEQ ID NOs. 34 to 37, SEQ ID NOs. 34 to 38, SEQ ID NOs. 34 to 39, SEQ ID NOs. 34 to 40, SEQ ID NOs. 34 to 41, SEQ ID NOs. 34 to 42, SEQ ID NOs. 34 to 43, SEQ ID NOs. 34 to 44, SEQ ID NOs. 34 to 45, SEQ ID NOs. 34 to 46, SEQ ID NOs. 34 to 47, SEQ ID NOs. 34 to 48, SEQ ID NOs. 34 to 49, SEQ ID NOs. 34 to 50, SEQ ID NOs. 34 to 51, SEQ ID NOs. 34 to 52, SEQ ID NOs. 34 to 53, and / or SEQ ID NOs. 34 to 54), preferably an amino acid sequence having at least 70% sequence identity with the amino acid sequence of SEQ ID NO. 34.

[0029] In some embodiments, the present invention relates to the use of at least two polypeptides for modifying trichothecenes, preferably deoxynivalenol and / or 3-keto-deoxynivalenol (especially for detoxifying them), wherein at least the first polypeptide comprises an amino acid sequence selected from SEQ ID NO.1 to SEQ ID NO.33 (such as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9, SEQ ID NO.10, SEQ ID NO.11, SEQ ID NO.12, SEQ ID NO.13, SEQ ID NO.14, SEQ ID NO.15, SEQ ID NO.16, SEQ ID NO.17, SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.21, SEQ ID NO.22, SEQ ID NO.23, SEQ ID NO.24, SEQ ID NO.25, SEQ ID NO.26, SEQ ID NO.27, SEQ ID NO.28, SEQ ID NO.29, SEQ ID NO.30, SEQ ID NO.31, SEQ ID NO.32, SEQ ID NO.33, SEQ ID NO.1 to SEQ ID NO.2, SEQ ID NO.1 to SEQ ID NO.3, SEQ ID NO.1 to SEQ ID NO.4, SEQ ID NO.1 to SEQ ID NO.5, SEQ ID NO.1 to SEQ ID NO.6, SEQ ID NO.1 to SEQ ID NO.7, SEQ ID NO.1 to SEQ ID NO.8, SEQ ID NO.1 to SEQ ID NO.9, SEQ ID NO.1 to SEQ ID NO.10, SEQ ID NO.1 to SEQ ID NO.11, SEQ ID NO.1 to SEQ ID NO.12, SEQ ID NO.1 to SEQ ID NO.13, SEQ ID NO.1 to SEQ ID NO.14, SEQ ID NO.1 to SEQ ID NO.15, SEQ ID NO.1 to SEQ ID NO.16, SEQ ID NO.1 to SEQ ID NO.17, SEQ ID NO.1 to SEQ ID NO.18, SEQ ID NO.1 to SEQ ID NO.19, SEQ ID NO.1 to SEQ ID NO.20. The amino acid sequence of SEQ ID NO.1 to SEQ ID NO.21, SEQ ID NO.1 to SEQ ID NO.22, SEQ ID NO.1 to SEQ ID NO.23, SEQ ID NO.1 to SEQ ID NO.24, SEQ ID NO.1 to SEQ ID NO.25, SEQ ID NO.1 to SEQ ID NO.26, SEQ ID NO.1 to SEQ ID NO.27, SEQ ID NO.1 to SEQ ID NO.28, SEQ ID NO.1 to SEQ ID NO.29, SEQ ID NO.1 to SEQ ID NO.30, SEQ ID NO.1 to SEQ ID NO.31, SEQ ID NO.1 to SEQ ID NO.32, and / or SEQ ID NO.1 to SEQ ID NO.33), preferably an amino acid sequence having at least 70% sequence identity with the amino acid sequence of SEQ ID NO.1; and wherein at least the second polypeptide comprises an amino acid sequence selected from SEQ ID NO.34 to SEQ ID NO.54 (such as SEQ ID NO.34, SEQ ID NO.35, SEQ ID NO.36, SEQ ID NO.37, SEQ ID NO.38, SEQ ID NO.39, SEQ ID NO.40, SEQ ID NO.41, SEQ ID NO.42, SEQ ID NO.43, SEQ ID NO.44, SEQ ID NO.45, SEQ ID NO.46, SEQ ID NO.47, SEQ ID NO.48, SEQ ID NO.49, SEQ ID NO.50, SEQ ID NO.51, SEQ ID NO.52, SEQ ID NO.53, SEQ ID NO.54, SEQ ID NO.34 to SEQ ID NO.35, SEQ ID NO.34 to SEQ ID NO.36, SEQ ID NO.34 to SEQ ID NO.37, SEQ ID NO.34 to SEQ ID NO.38, SEQ ID NO.34 to SEQ ID NO.39, SEQ ID NO.34 to SEQ ID NO.40, SEQ ID NO.34 to SEQ ID NO.41, SEQ ID NO.34 to SEQ ID NO.42, SEQ ID NO.34 to SEQ ID NO.43, SEQ ID NO.34 to SEQ ID NO.44, SEQ ID NO.34 to SEQ ID NO.45, SEQ ID NO.34 to SEQ ID NO.46. The amino acid sequence of SEQ ID NO. 34 to SEQ ID NO. 47, SEQ ID NO. 34 to SEQ ID NO. 48, SEQ ID NO. 34 to SEQ ID NO. 49, SEQ ID NO. 34 to SEQ ID NO. 50, SEQ ID NO. 34 to SEQ ID NO. 51, SEQ ID NO. 34 to SEQ ID NO. 52, SEQ ID NO. 34 to SEQ ID NO. 53, and / or SEQ ID NO. 34 to SEQ ID NO. 54), preferably an amino acid sequence having at least 70% sequence identity with the amino acid sequence of SEQ ID NO. 34.

[0030] In yet another aspect, the present invention relates to the use of at least one polypeptide for the manufacture of an additive for feed and / or food, a feed and / or food composition, or a pharmaceutical composition, wherein the at least one polypeptide comprises an amino acid sequence having at least 70% sequence identity with an amino acid sequence selected from SEQ ID NO. 1 to SEQ ID NO. 54, preferably selected from SEQ ID NO. 1 and SEQ ID NO. 34.

[0031] In some embodiments, the present invention relates to the use of at least one polypeptide for manufacturing an additive for feed and / or food, a feed and / or food composition, or a pharmaceutical composition, wherein the at least one polypeptide comprises a sequence selected from SEQ ID NO.1 to SEQ ID NO.33 (e.g., SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9, SEQ ID NO.10, SEQ ID NO.11, SEQ ID NO.12, SEQ ID NO.13, SEQ ID NO.14, SEQ ID NO.15, SEQ ID NO.16, SEQ ID NO.17, SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.21, SEQ ID NO.22, SEQ ID NO.23, SEQ ID NO.24, SEQ ID NO.25, SEQ ID NO.26, SEQ ID NO.27, SEQ ID NO.28, SEQ ID NO.29, SEQ ID NO.30, SEQ ID NO.31, SEQ ID NO.32, SEQ ID NO.33, SEQ ID NO.1 to SEQ ID NO.2, SEQ ID NO.1 to SEQ ID NO.3, SEQ ID NO.1 to SEQ ID NO.4, SEQ ID NO.1 to SEQ ID NO.5, SEQ ID NO.1 to SEQ ID NO.6, SEQ ID NO.1 to SEQ ID NO.7, SEQ ID NO.1 to SEQ ID NO.8, SEQ ID NO.1 to SEQ ID NO.9, SEQ ID NO.1 to SEQ ID NO.10, SEQ ID NO.1 to SEQ ID NO.11, SEQ ID NO.1 to SEQ ID NO.12, SEQ ID NO.1 to SEQ ID NO.13, SEQ ID NO.1 to SEQ ID NO.14, SEQ ID NO.1 to SEQ ID NO.15, SEQ ID NO.1 to SEQ ID NO.16, SEQ ID NO.1 to SEQ ID NO.17, SEQ ID NO.1 to SEQ ID NO.18, SEQ ID NO.1 to SEQ ID NO.19, SEQ ID NO.1 to SEQ ID NO.20, SEQ ID NO.1 to SEQ ID NO.21. The amino acid sequence of SEQ ID NO.1 to SEQ ID NO.22, SEQ ID NO.1 to SEQ ID NO.23, SEQ ID NO.1 to SEQ ID NO.24, SEQ ID NO.1 to SEQ ID NO.25, SEQ ID NO.1 to SEQ ID NO.26, SEQ ID NO.1 to SEQ ID NO.27, SEQ ID NO.1 to SEQ ID NO.28, SEQ ID NO.1 to SEQ ID NO.29, SEQ ID NO.1 to SEQ ID NO.30, SEQ ID NO.1 to SEQ ID NO.31, SEQ ID NO.1 to SEQ ID NO.32, and / or SEQ ID NO.1 to SEQ ID NO.33), preferably an amino acid sequence having at least 70% sequence identity with the amino acid sequence of SEQ ID NO.1.

[0032] In some embodiments, the present invention relates to the use of at least one polypeptide for manufacturing an additive for feed and / or food, a feed and / or food composition, or a pharmaceutical composition, wherein the at least one polypeptide comprises an amino acid sequence selected from SEQ ID NO.34 to SEQ ID NO.54 (such as SEQ ID NO.34, SEQ ID NO.35, SEQ ID NO.36, SEQ ID NO.37, SEQ ID NO.38, SEQ ID NO.39, SEQ ID NO.40, SEQ ID NO.41, SEQ ID NO.42, SEQ ID NO.43, SEQ ID NO.44, SEQ ID NO.45, SEQ ID NO.46, SEQ ID NO.47, SEQ ID NO.48, SEQ ID NO.49, SEQ ID NO.50, SEQ ID NO.51, SEQ ID NO.52, SEQ ID NO.53, SEQ ID NO.54, SEQ ID NO.34 to SEQ ID NO.35, SEQ ID NO.34 to SEQ ID NO.36, SEQ ID NO.34 to SEQ ID NO.37, SEQ ID NO.34 to SEQ ID NO.38, SEQ ID NO.34 to SEQ ID NO.39, SEQ ID NO.34 to SEQ ID NO.40, SEQ ID NO.34 to SEQ ID NO.41, SEQ ID NO.34 to SEQ ID NO.42, SEQ ID NO.34 to SEQ ID NO.43, SEQ ID NO.34 to SEQ ID NO.44, SEQ ID NO.34 to SEQ ID NO.45, SEQ ID NO.34 to SEQ ID NO.46, SEQ ID NO.34 to SEQ ID NO.47, SEQ ID NO.34 to SEQ ID NO.48, SEQ ID NO.34 to SEQ ID NO.49, SEQ ID NO.34 to SEQ ID NO.50, SEQ ID NO.34 to SEQ ID NO.51, SEQ ID NO.34 to SEQ ID NO.52, SEQ ID NO.34 to SEQ ID NO.53, and / or SEQ ID NO.34 to SEQ ID NO.54), preferably an amino acid sequence having at least 70% sequence identity with the amino acid sequence of SEQ ID NO.34.

[0033] In some embodiments, the present invention relates to the use of at least two polypeptides for manufacturing an additive for feed and / or food, a feed and / or food composition, or a pharmaceutical composition, wherein at least the first polypeptide comprises a sequence selected from SEQ ID NO.1 to SEQ ID NO.33 (such as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9, SEQ ID NO.10, SEQ ID NO.11, SEQ ID NO.12, SEQ ID NO.13, SEQ ID NO.14, SEQ ID NO.15, SEQ ID NO.16, SEQ ID NO.17, SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.21, SEQ ID NO.22, SEQ ID NO.23, SEQ ID NO.24, SEQ ID NO.25, SEQ ID NO.26, SEQ ID NO.27, SEQ ID NO.28, SEQ ID NO.29, SEQ ID NO.30, SEQ ID NO.31, SEQ ID NO.32, SEQ ID NO.33, SEQ ID NO.1 to SEQ ID NO.2, SEQ ID NO.1 to SEQ ID NO.3, SEQ ID NO.1 to SEQ ID NO.4, SEQ ID NO.1 to SEQ ID NO.5, SEQ ID NO.1 to SEQ ID NO.6, SEQ ID NO.1 to SEQ ID NO.7, SEQ ID NO.1 to SEQ ID NO.8, SEQ ID NO.1 to SEQ ID NO.9, SEQ ID NO.1 to SEQ ID NO.10, SEQ ID NO.1 to SEQ ID NO.11, SEQ ID NO.1 to SEQ ID NO.12, SEQ ID NO.1 to SEQ ID NO.13, SEQ ID NO.1 to SEQ ID NO.14, SEQ ID NO.1 to SEQ ID NO.15, SEQ ID NO.1 to SEQ ID NO.16, SEQ ID NO.1 to SEQ ID NO.17, SEQ ID NO.1 to SEQ ID NO.18, SEQ ID NO.1 to SEQ ID NO.19, SEQ ID NO.1 to SEQ ID NO.20, SEQ ID NO.1 to SEQ ID NO.21, the amino acid sequences of SEQ ID NO.1 to SEQ ID NO.22, SEQ ID NO.1 to SEQ ID NO.23, SEQ ID NO.1 to SEQ ID NO.24, SEQ ID NO.1 to SEQ ID NO.25, SEQ ID NO.1 to SEQ ID NO.26, SEQ ID NO.1 to SEQ ID NO.27, SEQ ID NO.1 to SEQ ID NO.28, SEQ ID NO.1 to SEQ ID NO.29, SEQ ID NO.1 to SEQ ID NO.30, SEQ ID NO.1 to SEQ ID NO.31, SEQ ID NO.1 to SEQ ID NO.32, and / or SEQ ID NO.1 to SEQ ID NO.33), preferably an amino acid sequence having at least 70% sequence identity with the amino acid sequence of SEQ ID NO.1; and wherein at least the second polypeptide comprises an amino acid sequence selected from SEQ ID NO.34 to SEQ ID NO.54 (such as SEQ ID NO.34, SEQ ID NO.35, SEQ ID NO.36, SEQ ID NO.37, SEQ ID NO.38, SEQ ID NO.39, SEQ ID NO.40, SEQ ID NO.41, SEQ ID NO.42, SEQ ID NO.43, SEQ ID NO.44, SEQ ID NO.45, SEQ ID NO.46, SEQ ID NO.47, SEQ ID NO.48, SEQ ID NO.49, SEQ ID NO.50, SEQ ID NO.51, SEQ ID NO.52, SEQ ID NO.53, SEQ ID NO.54, SEQ ID NO.34 to SEQ ID NO.35, SEQ ID NO.34 to SEQ ID NO.36, SEQ ID NO.34 to SEQ ID NO.37, SEQ ID NO.34 to SEQ ID NO.38, SEQ ID NO.34 to SEQ ID NO.39, SEQ ID NO.34 to SEQ ID NO.40, SEQ ID NO.34 to SEQ ID NO.41, SEQ ID NO.34 to SEQ ID NO.42, SEQ ID NO.34 to SEQ ID NO.43, SEQ ID NO.34 to SEQ ID NO.44, SEQ ID NO.34 to SEQ ID NO.45, SEQ ID NO.34 to SEQ ID NO.46, SEQ ID NO.34 to SEQ ID NO.47. The amino acid sequence of SEQ ID NO. 34 to SEQ ID NO. 48, SEQ ID NO. 34 to SEQ ID NO. 49, SEQ ID NO. 34 to SEQ ID NO. 50, SEQ ID NO. 34 to SEQ ID NO. 51, SEQ ID NO. 34 to SEQ ID NO. 52, SEQ ID NO. 34 to SEQ ID NO. 53, and / or SEQ ID NO. 34 to SEQ ID NO. 54), preferably an amino acid sequence having at least 70% sequence identity with the amino acid sequence of SEQ ID NO. 34.

[0034] In another aspect, the present invention relates to the use of at least one polypeptide for producing biogas; bioethanol, DDGS; sugar, preferably sugar from sugarcane or sugar beet; corn oil, corn germ, corn germ meal, corn fiber, corn gluten, starch, especially corn starch; and / or silage; wherein the at least one polypeptide comprises an amino acid sequence having at least 70% sequence identity with an amino acid sequence selected from SEQ ID NO. 1 to SEQ ID NO. 54, preferably selected from SEQ ID NO. 1 and SEQ ID NO. 34.

[0035] In some embodiments, the present invention relates to the use of at least one polypeptide for the production of biogas; bioethanol, DDGS; sugars, preferably sugars from sugarcane or sugar beet; corn oil, corn germ, corn germ meal, corn fiber, corn gluten, starch, especially corn starch; and / or silage; wherein the at least one polypeptide comprises a sequence selected from SEQ ID NO.1 to SEQ ID NO.33 (such as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9, SEQ ID NO.10, SEQ ID NO.11, SEQ ID NO.12, SEQ ID NO.13, SEQ ID NO.14, SEQ ID NO.15, SEQ ID NO.16, SEQ ID NO.17, SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.21, SEQ ID NO.22, SEQ ID NO.23, SEQ ID NO.24, SEQ ID NO.25, SEQ ID NO.26, SEQ ID NO.27, SEQ ID NO.28, SEQ ID NO.29, SEQ ID NO.30, SEQ ID NO.31, SEQ ID NO.32, SEQ ID NO.33, SEQ ID NO.1 to SEQ ID NO.2, SEQ ID NO.1 to SEQ ID NO.3, SEQ ID NO.1 to SEQ ID NO.4, SEQ ID NO.1 to SEQ ID NO.5, SEQ ID NO.1 to SEQ ID NO.6, SEQ ID NO.1 to SEQ ID NO.7, SEQ ID NO.1 to SEQ ID NO.8, SEQ ID NO.1 to SEQ ID NO.9, SEQ ID NO.1 to SEQ ID NO.10, SEQ ID NO.1 to SEQ ID NO.11, SEQ ID NO.1 to SEQ ID NO.12, SEQ ID NO.1 to SEQ ID NO.13, SEQ ID NO.1 to SEQ ID NO.14, SEQ ID NO.1 to SEQ ID NO.15, SEQ ID NO.1 to SEQ ID NO.16, SEQ ID NO.1 to SEQ ID NO.17, SEQ ID NO.1 to SEQ ID NO.18, SEQ ID NO.1 to SEQ ID NO.19, SEQ ID NO.The amino acid sequence of 1 to SEQ ID NO.20, SEQ ID NO.1 to SEQ ID NO.21, SEQ ID NO.1 to SEQ ID NO.22, SEQ ID NO.1 to SEQ ID NO.23, SEQ ID NO.1 to SEQ ID NO.24, SEQ ID NO.1 to SEQ ID NO.25, SEQ ID NO.1 to SEQ ID NO.26, SEQ ID NO.1 to SEQ ID NO.27, SEQ ID NO.1 to SEQ ID NO.28, SEQ ID NO.1 to SEQ ID NO.29, SEQ ID NO.1 to SEQ ID NO.30, SEQ ID NO.1 to SEQ ID NO.31, SEQ ID NO.1 to SEQ ID NO.32, and / or SEQ ID NO.1 to SEQ ID NO.33), preferably an amino acid sequence having at least 70% sequence identity with the amino acid sequence of SEQ ID NO.1.

[0036] In some embodiments, the present invention relates to the use of at least one polypeptide for the production of biogas; bioethanol, DDGS; sugar, preferably sugar from sugarcane or sugar beet; corn oil, corn germ, corn germ meal, corn fiber, corn gluten, starch, especially corn starch; and / or silage; wherein the at least one polypeptide comprises an amino acid sequence selected from SEQ ID NO. 34 to SEQ ID NO. 54 (such as SEQ ID NO. 34, SEQ ID NO. 35, SEQ ID NO. 36, SEQ ID NO. 37, SEQ ID NO. 38, SEQ ID NO. 39, SEQ ID NO. 40, SEQ ID NO. 41, SEQ ID NO. 42, SEQ ID NO. 43, SEQ ID NO. 44, SEQ ID NO. 45, SEQ ID NO. 46, SEQ ID NO. 47, SEQ ID NO. 48, SEQ ID NO. 49, SEQ ID NO. 50, SEQ ID NO. 51, SEQ ID NO. 52, SEQ ID NO. 53, SEQ ID NO. 54, SEQ ID NO. 34 to SEQ ID NO. 35, SEQ ID NO. 34 to SEQ ID NO. 36, SEQ ID NO. 34 to SEQ ID NO. 37, SEQ ID NO. 34 to SEQ ID NO. 38, SEQ ID NO. 34 to SEQ ID NO. 39, SEQ ID NO. 34 to SEQ ID NO. 40, SEQ ID NO. 34 to SEQ ID NO. 41, SEQ ID NO. 34 to SEQ ID NO. 42, SEQ ID NO. 34 to SEQ ID NO. 43, SEQ ID NO. 34 to SEQ ID NO. 44, SEQ ID NO. 34 to SEQ ID NO. 45, SEQ ID NO. 34 to SEQ ID NO. 46, SEQ ID NO. 34 to SEQ ID NO. 47, SEQ ID NO. 34 to SEQ ID NO. 48, SEQ ID NO. 34 to SEQ ID NO. 49, SEQ ID NO. 34 to SEQ ID NO. 50, SEQ ID NO. 34 to SEQ ID NO. 51, SEQ ID NO. 34 to SEQ ID NO. 52, SEQ ID NO. 34 to SEQ ID NO. 53, and / or SEQ ID NO. 34 to SEQ ID NO. 54), preferably an amino acid sequence having at least 70% sequence identity with the amino acid sequence of SEQ ID NO. 34.

[0037] In some embodiments, the present invention relates to the use of at least two polypeptides for the production of biogas; bioethanol, DDGS; sugars, preferably sugars from sugar cane or sugar beet; corn oil, corn germ, corn germ meal, corn fiber, corn gluten, starch, especially corn starch; and / or silage; wherein at least the first polypeptide comprises a sequence selected from SEQ ID NO.1 to SEQ ID NO.33 (such as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9, SEQ ID NO.10, SEQ ID NO.11, SEQ ID NO.12, SEQ ID NO.13, SEQ ID NO.14, SEQ ID NO.15, SEQ ID NO.16, SEQ ID NO.17, SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.21, SEQ ID NO.22, SEQ ID NO.23, SEQ ID NO.24, SEQ ID NO.25, SEQ ID NO.26, SEQ ID NO.27, SEQ ID NO.28, SEQ ID NO.29, SEQ ID NO.30, SEQ ID NO.31, SEQ ID NO.32, SEQ ID NO.33, SEQ ID NO.1 to SEQ ID NO.2, SEQ ID NO.1 to SEQ ID NO.3, SEQ ID NO.1 to SEQ ID NO.4, SEQ ID NO.1 to SEQ ID NO.5, SEQ ID NO.1 to SEQ ID NO.6, SEQ ID NO.1 to SEQ ID NO.7, SEQ ID NO.1 to SEQ ID NO.8, SEQ ID NO.1 to SEQ ID NO.9, SEQ ID NO.1 to SEQ ID NO.10, SEQ ID NO.1 to SEQ ID NO.11, SEQ ID NO.1 to SEQ ID NO.12, SEQ ID NO.1 to SEQ ID NO.13, SEQ ID NO.1 to SEQ ID NO.14, SEQ ID NO.1 to SEQ ID NO.15, SEQ ID NO.1 to SEQ ID NO.16, SEQ ID NO.1 to SEQ ID NO.17, SEQ ID NO.1 to SEQ ID NO.18, SEQ ID NO.1 to SEQ ID NO.19, SEQ ID NO.The amino acid sequence of 1 to SEQ ID NO.20, SEQ ID NO.1 to SEQ ID NO.21, SEQ ID NO.1 to SEQ ID NO.22, SEQ ID NO.1 to SEQ ID NO.23, SEQ ID NO.1 to SEQ ID NO.24, SEQ ID NO.1 to SEQ ID NO.25, SEQ ID NO.1 to SEQ ID NO.26, SEQ ID NO.1 to SEQ ID NO.27, SEQ ID NO.1 to SEQ ID NO.28, SEQ ID NO.1 to SEQ ID NO.29, SEQ ID NO.1 to SEQ ID NO.30, SEQ ID NO.1 to SEQ ID NO.31, SEQ ID NO.1 to SEQ ID NO.32, and / or SEQ ID NO.1 to SEQ ID NO.33), preferably an amino acid sequence having at least 70% sequence identity with the amino acid sequence of SEQ ID NO.1; and wherein at least the second polypeptide comprises an amino acid sequence selected from SEQ ID NO.34 to SEQ ID NO.54 (such as SEQ ID NO.34, SEQ ID NO.35, SEQ ID NO.36, SEQ ID NO.37, SEQ ID NO.38, SEQ ID NO.39, SEQ ID NO.40, SEQ ID NO.41, SEQ ID NO.42, SEQ ID NO.43, SEQ ID NO.44, SEQ ID NO.45, SEQ ID NO.46, SEQ ID NO.47, SEQ ID NO.48, SEQ ID NO.49, SEQ ID NO.50, SEQ ID NO.51, SEQ ID NO.52, SEQ ID NO.53, SEQ ID NO.54, SEQ ID NO.34 to SEQ ID NO.35, SEQ ID NO.34 to SEQ ID NO.36, SEQ ID NO.34 to SEQ ID NO.37, SEQ ID NO.34 to SEQ ID NO.38, SEQ ID NO.34 to SEQ ID NO.39, SEQ ID NO.34 to SEQ ID NO.40, SEQ ID NO.34 to SEQ ID NO.41, SEQ ID NO.34 to SEQ ID NO.42, SEQ ID NO.34 to SEQ ID NO.43, SEQ ID NO.34 to SEQ ID NO.44, SEQ ID NO.34 to SEQ ID NO.45, SEQ ID NO.the amino acid sequences of SEQ ID NO. 34 to SEQ ID NO. 46, SEQ ID NO. 34 to SEQ ID NO. 47, SEQ ID NO. 34 to SEQ ID NO. 48, SEQ ID NO. 34 to SEQ ID NO. 49, SEQ ID NO. 34 to SEQ ID NO. 50, SEQ ID NO. 34 to SEQ ID NO. 51, SEQ ID NO. 34 to SEQ ID NO. 52, SEQ ID NO. 34 to SEQ ID NO. 53, and / or SEQ ID NO. 34 to SEQ ID NO. 54), preferably an amino acid sequence having at least 70% sequence identity with the amino acid sequence of SEQ ID NO. 34.

[0038] In yet another aspect, the present invention relates to a host cell comprising at least one polypeptide and / or at least one polynucleotide encoding the at least one polypeptide, wherein the at least one polypeptide comprises an amino acid sequence selected from SEQ ID NO.1 to SEQ ID NO.54 (e.g., SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9, SEQ ID NO.10, SEQ ID NO.11, SEQ ID NO.12, SEQ ID NO.13, SEQ ID NO.14, SEQ ID NO.15, SEQ ID NO.16, SEQ ID NO.17, SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.21, SEQ ID NO.22, SEQ ID NO.23, SEQ ID NO.24, SEQ ID NO.25, SEQ ID NO.26, SEQ ID NO.27, SEQ ID NO.28, SEQ ID NO.29, SEQ ID NO.30, SEQ ID NO.31, SEQ ID NO.32, SEQ ID NO.33, SEQ ID NO.34, SEQ ID NO.35, SEQ ID NO.36, SEQ ID NO.37, SEQ ID NO.38, SEQ ID NO.39, SEQ ID NO.40, SEQ ID NO.41, SEQ ID NO.42, SEQ ID NO.43, SEQ ID NO.44, SEQ ID NO.45, SEQ ID NO.46, SEQ ID NO.47, SEQ ID NO.48, SEQ ID NO.49, SEQ ID NO.50, SEQ ID NO.51, SEQ ID NO.52, SEQ ID NO.53, SEQ ID NO.54, SEQ ID NO.1 to SEQ ID NO.54, SEQ ID NO.1 to SEQ ID NO.2, SEQ ID NO.1 to SEQ ID NO.3, SEQ ID NO.1 to SEQ ID NO.4, SEQ ID NO.1 to SEQ ID NO.5, SEQ ID NO.1 to SEQ ID NO.6, SEQ ID NO.1 to SEQ ID NO.7, SEQ ID NO.1 to SEQ ID NO.8, SEQ ID NO.1 to SEQ ID NO.9. SEQ ID NO.1 to SEQ ID NO.10, SEQ ID NO.1 to SEQ ID NO.11, SEQ ID NO.1 to SEQ ID NO.12, SEQ ID NO.1 to SEQ ID NO.13, SEQ ID NO.1 to SEQ ID NO.14, SEQ ID NO.1 to SEQ ID NO.15, SEQ ID NO.1 to SEQ ID NO.16, SEQ ID NO.1 to SEQ ID NO.17, SEQ ID NO.1 to SEQ ID NO.18, SEQ ID NO.1 to SEQ ID NO.19, SEQ ID NO.1 to SEQ ID NO.20, SEQ ID NO.1 to SEQ ID NO.21, SEQ ID NO.1 to SEQ ID NO.22, SEQ ID NO.1 to SEQ ID NO.23, SEQ ID NO.1 to SEQ ID NO.24, SEQ ID NO.1 to SEQ ID NO.25, SEQ ID NO.1 to SEQ ID NO.26, SEQ ID NO.1 to SEQ ID NO.27, SEQ ID NO.1 to SEQ ID NO.28, SEQ ID NO.1 to SEQ ID NO.29, SEQ ID NO.1 to SEQ ID NO.30, SEQ ID NO.1 to SEQ ID NO.31, SEQ ID NO.1 to SEQ ID NO.32, SEQ ID NO.1 to SEQ ID NO.33, SEQ ID NO.34 to SEQ ID NO.35, SEQ ID NO.34 to SEQ ID NO.36, SEQ ID NO.34 to SEQ ID NO.37, SEQ ID NO.34 to SEQ ID NO.38, SEQ ID NO.34 to SEQ ID NO.39, SEQ ID NO.34 to SEQ ID NO.40, SEQ ID NO.34 to SEQ ID NO.41, SEQ ID NO.34 to SEQ ID NO.42, SEQ ID NO.34 to SEQ ID NO.43, SEQ ID NO.34 to SEQ ID NO.44, SEQ ID NO.34 to SEQ ID NO.45, SEQ ID NO.34 to SEQ ID NO.46, SEQ ID NO.34 to SEQ ID NO.47, SEQ ID NO.34 to SEQ ID NO.48, SEQ ID NO.34 to SEQ ID NO.49, SEQ ID NO.from SEQ ID NO. 34 to SEQ ID NO. 50, SEQ ID NO. 34 to SEQ ID NO. 51, SEQ ID NO. 34 to SEQ ID NO. 52, SEQ ID NO. 34 to SEQ ID NO. 53, and / or SEQ ID NO. 34 to SEQ ID NO. 54), preferably an amino acid sequence having at least 70% sequence identity to the amino acid sequences of SEQ ID NO. 1 and SEQ ID NO. 34. Such a host cell according to the invention allows the production of the polypeptides mentioned herein, in particular the recombinant, heterologous production of the polypeptides mentioned herein.

[0039] In some embodiments, the present invention relates to a host cell comprising at least two polypeptides, wherein at least the first polypeptide comprises a sequence selected from SEQ ID NO.1 to SEQ ID NO.33 (such as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9, SEQ ID NO.10, SEQ ID NO.11, SEQ ID NO.12, SEQ ID NO.13, SEQ ID NO.14, SEQ ID NO.15, SEQ ID NO.16, SEQ ID NO.17, SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.21, SEQ ID NO.22, SEQ ID NO.23, SEQ ID NO.24, SEQ ID NO.25, SEQ ID NO.26, SEQ ID NO.27, SEQ ID NO.28, SEQ ID NO.29, SEQ ID NO.30, SEQ ID NO.31, SEQ ID NO.32, SEQ ID NO.33, SEQ ID NO.1 to SEQ ID NO.2, SEQ ID NO.1 to SEQ ID NO.3, SEQ ID NO.1 to SEQ ID NO.4, SEQ ID NO.1 to SEQ ID NO.5, SEQ ID NO.1 to SEQ ID NO.6, SEQ ID NO.1 to SEQ ID NO.7, SEQ ID NO.1 to SEQ ID NO.8, SEQ ID NO.1 to SEQ ID NO.9, SEQ ID NO.1 to SEQ ID NO.10, SEQ ID NO.1 to SEQ ID NO.11, SEQ ID NO.1 to SEQ ID NO.12, SEQ ID NO.1 to SEQ ID NO.13, SEQ ID NO.1 to SEQ ID NO.14, SEQ ID NO.1 to SEQ ID NO.15, SEQ ID NO.1 to SEQ ID NO.16, SEQ ID NO.1 to SEQ ID NO.17, SEQ ID NO.1 to SEQ ID NO.18, SEQ ID NO.1 to SEQ ID NO.19, SEQ ID NO.1 to SEQ ID NO.20, SEQ ID NO.1 to SEQ ID NO.21, SEQ ID NO.1 to SEQ ID NO.22. The amino acid sequence of SEQ ID NO.1 to SEQ ID NO.23, SEQ ID NO.1 to SEQ ID NO.24, SEQ ID NO.1 to SEQ ID NO.25, SEQ ID NO.1 to SEQ ID NO.26, SEQ ID NO.1 to SEQ ID NO.27, SEQ ID NO.1 to SEQ ID NO.28, SEQ ID NO.1 to SEQ ID NO.29, SEQ ID NO.1 to SEQ ID NO.30, SEQ ID NO.1 to SEQ ID NO.31, SEQ ID NO.1 to SEQ ID NO.32, and / or SEQ ID NO.1 to SEQ ID NO.33), preferably an amino acid sequence having at least 70% sequence identity with the amino acid sequence of SEQ ID NO.1; and wherein at least the second polypeptide comprises an amino acid sequence selected from SEQ ID NO.34 to SEQ ID NO.54 (e.g., SEQ ID NO.34, SEQ ID NO.35, SEQ ID NO.36, SEQ ID NO.37, SEQ ID NO.38, SEQ ID NO.39, SEQ ID NO.40, SEQ ID NO.41, SEQ ID NO.42, SEQ ID NO.43, SEQ ID NO.44, SEQ ID NO.45, SEQ ID NO.46, SEQ ID NO.47, SEQ ID NO.48, SEQ ID NO.49, SEQ ID NO.50, SEQ ID NO.51, SEQ ID NO.52, SEQ ID NO.53, SEQ ID NO.54, SEQ ID NO.34 to SEQ ID NO.35, SEQ ID NO.34 to SEQ ID NO.36, SEQ ID NO.34 to SEQ ID NO.37, SEQ ID NO.34 to SEQ ID NO.38, SEQ ID NO.34 to SEQ ID NO.39, SEQ ID NO.34 to SEQ ID NO.40, SEQ ID NO.34 to SEQ ID NO.41, SEQ ID NO.34 to SEQ ID NO.42, SEQ ID NO.34 to SEQ ID NO.43, SEQ ID NO.34 to SEQ ID NO.44, SEQ ID NO.34 to SEQ ID NO.45, SEQ ID NO.34 to SEQ ID NO.46, SEQ ID NO.34 to SEQ ID NO.47, SEQ ID NO.34 to SEQ ID NO.The amino acid sequences of SEQ ID NO. 34 to SEQ ID NO. 49, SEQ ID NO. 34 to SEQ ID NO. 50, SEQ ID NO. 34 to SEQ ID NO. 51, SEQ ID NO. 34 to SEQ ID NO. 52, SEQ ID NO. 34 to SEQ ID NO. 53, and / or SEQ ID NO. 34 to SEQ ID NO. 54), preferably amino acid sequences having at least 70% sequence identity with the amino acid sequence of SEQ ID NO. 34.

[0040] The term "host cell" refers to any cell capable of producing a recombinant protein. Specifically, a "host cell" refers to a prokaryotic cell and / or a eukaryotic cell, preferably Pichia pastoris, Escherichia coli, Bacillus sp. (such as Bacillus subtilis or Bacillus amyloliquefaciens), Streptomyces sp., Hansenula sp., Trichoderma sp., Lactobacillus sp., Aspergillus sp., plant cells, and / or spores of the genus Bacillus, Trichoderma, or Aspergillus. It should be noted that strains commonly associated with "Pichia pastoris" are sometimes referred to as Komagataella pastoris, Komagataella phaffii, or Komagataella pseudopastoris, depending on the corresponding taxonomic system at the time of reference.

[0041] In another aspect, the present invention relates to a polypeptide for modifying at least one trichothecene (e.g., detoxifying at least one trichothecene), the polypeptide comprising an amino acid sequence selected from SEQ ID NO.1 to SEQ ID NO.22 (e.g., SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9, SEQ ID NO.10, SEQ ID NO.11, SEQ ID NO.12, SEQ ID NO.13, SEQ ID NO.14, SEQ ID NO.15, SEQ ID NO.16, SEQ ID NO.17, SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.21, SEQ ID NO.22, SEQ ID NO.1 to SEQ ID NO.2, SEQ ID NO.1 to SEQ ID NO.3, SEQ ID NO.1 to SEQ ID NO.4, SEQ ID NO.1 to SEQ ID NO.5, SEQ ID NO.1 to SEQ ID NO.6, SEQ ID NO.1 to SEQ ID NO.7, SEQ ID NO.1 to SEQ ID NO.8, SEQ ID NO.1 to SEQ ID NO.9, SEQ ID NO.1 to SEQ ID NO.10, SEQ ID NO.1 to SEQ ID NO.11, SEQ ID NO.1 to SEQ ID NO.12, SEQ ID NO.1 to SEQ ID NO.13, SEQ ID NO.1 to SEQ ID NO.14, SEQ ID NO.1 to SEQ ID NO.15, SEQ ID NO.1 to SEQ ID NO.16, SEQ ID NO.1 to SEQ ID NO.17, SEQ ID NO.1 to SEQ ID NO.18, SEQ ID NO.1 to SEQ ID NO.19, SEQ ID NO.1 to SEQ ID NO.20, SEQ ID NO.1 to SEQ ID NO.21, and / or SEQ ID NO.1 to SEQ ID NO.22), preferably having at least 96.0% sequence identity with SEQ ID NO.1; or selected from SEQ ID NO.34 to SEQ ID NO.54 (e.g., SEQ ID NO.34, SEQ ID NO.35, SEQ ID NO.36, SEQ ID NO.37, SEQ ID NO.the amino acid sequences of SEQ ID NO.38, SEQ ID NO.39, SEQ ID NO.40, SEQ ID NO.41, SEQ ID NO.42, SEQ ID NO.43, SEQ ID NO.44, SEQ ID NO.45, SEQ ID NO.46, SEQ ID NO.47, SEQ ID NO.48, SEQ ID NO.49, SEQ ID NO.50, SEQ ID NO.51, SEQ ID NO.52, SEQ ID NO.53, SEQ ID NO.54, SEQ ID NO.34 to SEQ ID NO.35, SEQ ID NO.34 to SEQ ID NO.36, SEQ ID NO.34 to SEQ ID NO.37, SEQ ID NO.34 to SEQ ID NO.38, SEQ ID NO.34 to SEQ ID NO.39, SEQ ID NO.34 to SEQ ID NO.40, SEQ ID NO.34 to SEQ ID NO.41, SEQ ID NO.34 to SEQ ID NO.42, SEQ ID NO.34 to SEQ ID NO.43, SEQ ID NO.34 to SEQ ID NO.44, SEQ ID NO.34 to SEQ ID NO.45, SEQ ID NO.34 to SEQ ID NO.46, SEQ ID NO.34 to SEQ ID NO.47, SEQ ID NO.34 to SEQ ID NO.48, SEQ ID NO.34 to SEQ ID NO.49, SEQ ID NO.34 to SEQ ID NO.50, SEQ ID NO.34 to SEQ ID NO.51, SEQ ID NO.34 to SEQ ID NO.52, SEQ ID NO.34 to SEQ ID NO.53, and / or SEQ ID NO.34 to SEQ ID NO.54), preferably an amino acid sequence having at least 91.0% sequence identity with SEQ ID NO.34.

[0042] In some embodiments, the present invention relates to polypeptides for modifying at least one trichothene, in particular deoxynivalenol (e.g., detoxifying it), said polypeptides comprising an amino acid sequence selected from SEQ ID NO.1 to SEQ ID NO.22 (such as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9, SEQ ID NO.10, SEQ ID NO.11, SEQ ID NO.12, SEQ ID NO.13, SEQ ID NO.14, SEQ ID NO.15, SEQ ID NO.16, SEQ ID NO.17, SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.21, SEQ ID NO.22, SEQ ID NO.1 to SEQ ID NO.2, SEQ ID NO.1 to SEQ ID NO.3, SEQ ID NO.1 to SEQ ID NO.4, SEQ ID NO.1 to SEQ ID NO.5, SEQ ID NO.1 to SEQ ID NO.6, SEQ ID NO.1 to SEQ ID NO.7, SEQ ID NO.1 to SEQ ID NO.8, SEQ ID NO.1 to SEQ ID NO.9, SEQ ID NO.1 to SEQ ID NO.10, SEQ ID NO.1 to SEQ ID NO.11, SEQ ID NO.1 to SEQ ID NO.12, SEQ ID NO.1 to SEQ ID NO.13, SEQ ID NO.1 to SEQ ID NO.14, SEQ ID NO.1 to SEQ ID NO.15, SEQ ID NO.1 to SEQ ID NO.16, SEQ ID NO.1 to SEQ ID NO.17, SEQ ID NO.1 to SEQ ID NO.18, SEQ ID NO.1 to SEQ ID NO.19, SEQ ID NO.1 to SEQ ID NO.20, SEQ ID NO.1 to SEQ ID NO.21, and / or SEQ ID NO.1 to SEQ ID NO.22), preferably an amino acid sequence having at least 96.0% sequence identity with SEQ ID NO.1.

[0043] In some embodiments, the present invention relates to polypeptides for modifying at least one trichothene, in particular 3-keto-deoxynivalenol (e.g., detoxifying it), said polypeptides comprising an amino acid sequence selected from SEQ ID NO. 34 to SEQ ID NO. 54 (e.g., SEQ ID NO. 34, SEQ ID NO. 35, SEQ ID NO. 36, SEQ ID NO. 37, SEQ ID NO. 38, SEQ ID NO. 39, SEQ ID NO. 40, SEQ ID NO. 41, SEQ ID NO. 42, SEQ ID NO. 43, SEQ ID NO. 44, SEQ ID NO. 45, SEQ ID NO. 46, SEQ ID NO. 47, SEQ ID NO. 48, SEQ ID NO. 49, SEQ ID NO. 50, SEQ ID NO. 51, SEQ ID NO. 52, SEQ ID NO. 53, SEQ ID NO. 54, SEQ ID NO. 34 to SEQ ID NO. 35, SEQ ID NO. 34 to SEQ ID NO. 36, SEQ ID NO. 34 to SEQ ID NO. 37, SEQ ID NO. 34 to SEQ ID NO. 38, SEQ ID NO. 34 to SEQ ID NO. 39, SEQ ID NO. 34 to SEQ ID NO. 40, SEQ ID NO. 34 to SEQ ID NO. 41, SEQ ID NO. 34 to SEQ ID NO. 42, SEQ ID NO. 34 to SEQ ID NO. 43, SEQ ID NO. 34 to SEQ ID NO. 44, SEQ ID NO. 34 to SEQ ID NO. 45, SEQ ID NO. 34 to SEQ ID NO. 46, SEQ ID NO. 34 to SEQ ID NO. 47, SEQ ID NO. 34 to SEQ ID NO. 48, SEQ ID NO. 34 to SEQ ID NO. 49, SEQ ID NO. 34 to SEQ ID NO. 50, SEQ ID NO. 34 to SEQ ID NO. 51, SEQ ID NO. 34 to SEQ ID NO. 52, SEQ ID NO. 34 to SEQ ID NO. 53, and / or SEQ ID NO. 34 to SEQ ID NO. 54), preferably an amino acid sequence having at least 91.0% sequence identity with SEQ ID NO. 34.

[0044] In yet another aspect, the present invention relates to at least one polypeptide for treating, ameliorating and / or preventing symptoms caused by mycotoxin poisoning, in particular trichothecene mycotoxin poisoning, wherein said at least one polypeptide comprises an amino acid sequence having at least 70% sequence identity with an amino acid sequence selected from SEQ ID NO.1 to SEQ ID NO.54, preferably selected from SEQ ID NO.1 and SEQ ID NO.34.

[0045] In some embodiments, the present invention relates to at least one polypeptide for treating, ameliorating, and / or preventing symptoms caused by mycotoxin poisoning, particularly trichothecene mycotoxin poisoning (e.g., deoxynivalenol mycotoxin poisoning), wherein the at least one polypeptide comprises a sequence selected from SEQ ID NO.1 to SEQ ID NO.33 (e.g., SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9, SEQ ID NO.10, SEQ ID NO.11, SEQ ID NO.12, SEQ ID NO.13, SEQ ID NO.14, SEQ ID NO.15, SEQ ID NO.16, SEQ ID NO.17, SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.21, SEQ ID NO.22, SEQ ID NO.23, SEQ ID NO.24, SEQ ID NO.25, SEQ ID NO.26, SEQ ID NO.27, SEQ ID NO.28, SEQ ID NO.29, SEQ ID NO.30, SEQ ID NO.31, SEQ ID NO.32, SEQ ID NO.33, SEQ ID NO.1 to SEQ ID NO.2, SEQ ID NO.1 to SEQ ID NO.3, SEQ ID NO.1 to SEQ ID NO.4, SEQ ID NO.1 to SEQ ID NO.5, SEQ ID NO.1 to SEQ ID NO.6, SEQ ID NO.1 to SEQ ID NO.7, SEQ ID NO.1 to SEQ ID NO.8, SEQ ID NO.1 to SEQ ID NO.9, SEQ ID NO.1 to SEQ ID NO.10, SEQ ID NO.1 to SEQ ID NO.11, SEQ ID NO.1 to SEQ ID NO.12, SEQ ID NO.1 to SEQ ID NO.13, SEQ ID NO.1 to SEQ ID NO.14, SEQ ID NO.1 to SEQ ID NO.15, SEQ ID NO.1 to SEQ ID NO.16, SEQ ID NO.1 to SEQ ID NO.17, SEQ ID NO.1 to SEQ ID NO.18, SEQ ID NO.1 to SEQ ID NO.19, SEQ ID NO.1 to SEQ ID NO.20. The amino acid sequence of SEQ ID NO.1 to SEQ ID NO.21, SEQ ID NO.1 to SEQ ID NO.22, SEQ ID NO.1 to SEQ ID NO.23, SEQ ID NO.1 to SEQ ID NO.24, SEQ ID NO.1 to SEQ ID NO.25, SEQ ID NO.1 to SEQ ID NO.26, SEQ ID NO.1 to SEQ ID NO.27, SEQ ID NO.1 to SEQ ID NO.28, SEQ ID NO.1 to SEQ ID NO.29, SEQ ID NO.1 to SEQ ID NO.30, SEQ ID NO.1 to SEQ ID NO.31, SEQ ID NO.1 to SEQ ID NO.32, and / or SEQ ID NO.1 to SEQ ID NO.33), preferably an amino acid sequence having at least 70% sequence identity with the amino acid sequence of SEQ ID NO.1.

[0046] In some embodiments, the present invention relates to at least one polypeptide for treating, ameliorating, and / or preventing symptoms caused by mycotoxin poisoning, particularly trichothecene mycotoxin poisoning (e.g., 3-keto-deoxynivalenol mycotoxin poisoning), wherein the at least one polypeptide comprises an amino acid sequence selected from SEQ ID NO. 34 to SEQ ID NO. 54 (e.g., SEQ ID NO. 34, SEQ ID NO. 35, SEQ ID NO. 36, SEQ ID NO. 37, SEQ ID NO. 38, SEQ ID NO. 39, SEQ ID NO. 40, SEQ ID NO. 41, SEQ ID NO. 42, SEQ ID NO. 43, SEQ ID NO. 44, SEQ ID NO. 45, SEQ ID NO. 46, SEQ ID NO. 47, SEQ ID NO. 48, SEQ ID NO. 49, SEQ ID NO. 50, SEQ ID NO. 51, SEQ ID NO. 52, SEQ ID NO. 53, SEQ ID NO. 54, SEQ ID NO. 34 to SEQ ID NO. 35, SEQ ID NO. 34 to SEQ ID NO. 36, SEQ ID NO. 34 to SEQ ID NO. 37, SEQ ID NO. 34 to SEQ ID NO. 38, SEQ ID NO. 34 to SEQ ID NO. 39, SEQ ID NO. 34 to SEQ ID NO. 40, SEQ ID NO. 34 to SEQ ID NO. 41, SEQ ID NO. 34 to SEQ ID NO. 42, SEQ ID NO. 34 to SEQ ID NO. 43, SEQ ID NO. 34 to SEQ ID NO. 44, SEQ ID NO. 34 to SEQ ID NO. 45, SEQ ID NO. 34 to SEQ ID NO. 46, SEQ ID NO. 34 to SEQ ID NO. 47, SEQ ID NO. 34 to SEQ ID NO. 48, SEQ ID NO. 34 to SEQ ID NO. 49, SEQ ID NO. 34 to SEQ ID NO. 50, SEQ ID NO. 34 to SEQ ID NO. 51, SEQ ID NO. 34 to SEQ ID NO. 52, SEQ ID NO. 34 to SEQ ID NO. 53, and / or SEQ ID NO. 34 to SEQ ID NO. 54), preferably an amino acid sequence having at least 70% sequence identity with the amino acid sequence of SEQ ID NO. 34.

[0047] In some embodiments, the present invention relates to at least two polypeptides for treating, ameliorating, and / or preventing symptoms caused by mycotoxin poisoning, particularly trichothecene mycotoxin poisoning (e.g., deoxynivalenol and / or 3-keto-deoxynivalenol mycotoxin poisoning), wherein at least the first polypeptide comprises an amino acid sequence selected from SEQ ID NO.1 to SEQ ID NO.33 (e.g., SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9, SEQ ID NO.10, SEQ ID NO.11, SEQ ID NO.12, SEQ ID NO.13, SEQ ID NO.14, SEQ ID NO.15, SEQ ID NO.16, SEQ ID NO.17, SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.21, SEQ ID NO.22, SEQ ID NO.23, SEQ ID NO.24, SEQ ID NO.25, SEQ ID NO.26, SEQ ID NO.27, SEQ ID NO.28, SEQ ID NO.29, SEQ ID NO.30, SEQ ID NO.31, SEQ ID NO.32, SEQ ID NO.33, SEQ ID NO.1 to SEQ ID NO.2, SEQ ID NO.1 to SEQ ID NO.3, SEQ ID NO.1 to SEQ ID NO.4, SEQ ID NO.1 to SEQ ID NO.5, SEQ ID NO.1 to SEQ ID NO.6, SEQ ID NO.1 to SEQ ID NO.7, SEQ ID NO.1 to SEQ ID NO.8, SEQ ID NO.1 to SEQ ID NO.9, SEQ ID NO.1 to SEQ ID NO.10, SEQ ID NO.1 to SEQ ID NO.11, SEQ ID NO.1 to SEQ ID NO.12, SEQ ID NO.1 to SEQ ID NO.13, SEQ ID NO.1 to SEQ ID NO.14, SEQ ID NO.1 to SEQ ID NO.15, SEQ ID NO.1 to SEQ ID NO.16, SEQ ID NO.1 to SEQ ID NO.17, SEQ ID NO.1 to SEQ ID NO.18, SEQ ID NO.1 to SEQ ID NO.19, SEQ ID NO.The amino acid sequence of 1 to SEQ ID NO.20, SEQ ID NO.1 to SEQ ID NO.21, SEQ ID NO.1 to SEQ ID NO.22, SEQ ID NO.1 to SEQ ID NO.23, SEQ ID NO.1 to SEQ ID NO.24, SEQ ID NO.1 to SEQ ID NO.25, SEQ ID NO.1 to SEQ ID NO.26, SEQ ID NO.1 to SEQ ID NO.27, SEQ ID NO.1 to SEQ ID NO.28, SEQ ID NO.1 to SEQ ID NO.29, SEQ ID NO.1 to SEQ ID NO.30, SEQ ID NO.1 to SEQ ID NO.31, SEQ ID NO.1 to SEQ ID NO.32, and / or SEQ ID NO.1 to SEQ ID NO.33), preferably an amino acid sequence having at least 70% sequence identity with the amino acid sequence of SEQ ID NO.1; and wherein at least the second polypeptide comprises an amino acid sequence selected from SEQ ID NO.34 to SEQ ID NO.54 (such as SEQ ID NO.34, SEQ ID NO.35, SEQ ID NO.36, SEQ ID NO.37, SEQ ID NO.38, SEQ ID NO.39, SEQ ID NO.40, SEQ ID NO.41, SEQ ID NO.42, SEQ ID NO.43, SEQ ID NO.44, SEQ ID NO.45, SEQ ID NO.46, SEQ ID NO.47, SEQ ID NO.48, SEQ ID NO.49, SEQ ID NO.50, SEQ ID NO.51, SEQ ID NO.52, SEQ ID NO.53, SEQ ID NO.54, SEQ ID NO.34 to SEQ ID NO.35, SEQ ID NO.34 to SEQ ID NO.36, SEQ ID NO.34 to SEQ ID NO.37, SEQ ID NO.34 to SEQ ID NO.38, SEQ ID NO.34 to SEQ ID NO.39, SEQ ID NO.34 to SEQ ID NO.40, SEQ ID NO.34 to SEQ ID NO.41, SEQ ID NO.34 to SEQ ID NO.42, SEQ ID NO.34 to SEQ ID NO.43, SEQ ID NO.34 to SEQ ID NO.44, SEQ ID NO.34 to SEQ ID NO.45, SEQ ID NO.The amino acid sequence of SEQ ID NO. 34 to SEQ ID NO. 46, SEQ ID NO. 34 to SEQ ID NO. 47, SEQ ID NO. 34 to SEQ ID NO. 48, SEQ ID NO. 34 to SEQ ID NO. 49, SEQ ID NO. 34 to SEQ ID NO. 50, SEQ ID NO. 34 to SEQ ID NO. 51, SEQ ID NO. 34 to SEQ ID NO. 52, SEQ ID NO. 34 to SEQ ID NO. 53, and / or SEQ ID NO. 34 to SEQ ID NO. 54), preferably an amino acid sequence having at least 70% sequence identity with the amino acid sequence of SEQ ID NO. 34.

[0048] The present invention is further characterized by the following items:

[0049] Item 1: A method for modifying at least one trichothecene, preferably deoxynivalenol (DON) and / or 3-keto-deoxynivalenol (3-keto-DON), for example, detoxifying it, the method comprising forming a reaction mixture by contacting at least one polypeptide with a nutrient composition containing at least one trichothecene, and with NAD(H) and / or NADP(H), preferably with NAD(H), the at least one polypeptide comprising an amino acid sequence having at least 70% (such as at least 70%, 71%, 72%, 73%, 74, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100%) sequence identity with an amino acid sequence selected from SEQ ID NO. 1 to SEQ ID NO. 54, preferably selected from SEQ ID NO. 1 and SEQ ID NO. 34.

[0050] Item 2. A method for modifying at least one trichothene, preferably deoxynivalenol (DON), e.g., detoxifying it, the method comprising forming a reaction mixture by contacting at least one polypeptide with a nutritional composition comprising at least one trichothene and with NAD(H) and / or NADP(H), preferably with NAD(H), the at least one polypeptide comprising an amino acid sequence selected from SEQ ID NO.1 to SEQ ID NO.33 (such as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9, SEQ ID NO.10, SEQ ID NO.11, SEQ ID NO.12, SEQ ID NO.13, SEQ ID NO.14, SEQ ID NO.15, SEQ ID NO.16, SEQ ID NO.17, SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.21, SEQ ID NO.22, SEQ ID NO.23, SEQ ID NO.24, SEQ ID NO.25, SEQ ID NO.26, SEQ ID NO.27, SEQ ID NO.28, SEQ ID NO.29, SEQ ID NO.30, SEQ ID NO.31, SEQ ID NO.32, SEQ ID NO.33, SEQ ID NO.1 to SEQ ID NO.2, SEQ ID NO.1 to SEQ ID NO.3, SEQ ID NO.1 to SEQ ID NO.4, SEQ ID NO.1 to SEQ ID NO.5, SEQ ID NO.1 to SEQ ID NO.6, SEQ ID NO.1 to SEQ ID NO.7, SEQ ID NO.1 to SEQ ID NO.8, SEQ ID NO.1 to SEQ ID NO.9, SEQ ID NO.1 to SEQ ID NO.10, SEQ ID NO.1 to SEQ ID NO.11, SEQ ID NO.1 to SEQ ID NO.12, SEQ ID NO.1 to SEQ ID NO.13, SEQ ID NO.1 to SEQ ID NO.14, SEQ ID NO.1 to SEQ ID NO.15, SEQ ID NO.1 to SEQ ID NO.16, SEQ ID NO.1 to SEQ ID NO.17, SEQ ID NO.1 to SEQ ID NO.18, the amino acid sequence of SEQ ID NO.1 to SEQ ID NO.19, SEQ ID NO.1 to SEQ ID NO.20, SEQ ID NO.1 to SEQ ID NO.21, SEQ ID NO.1 to SEQ ID NO.22, SEQ ID NO.1 to SEQ ID NO.23, SEQ ID NO.1 to SEQ ID NO.24, SEQ ID NO.1 to SEQ ID NO.25, SEQ ID NO.1 to SEQ ID NO.26, SEQ ID NO.1 to SEQ ID NO.27, SEQ ID NO.1 to SEQ ID NO.28, SEQ ID NO.1 to SEQ ID NO.29, SEQ ID NO.1 to SEQ ID NO.30, SEQ ID NO.1 to SEQ ID NO.31, SEQ ID NO.1 to SEQ ID NO.32, and / or SEQ ID NO.1 to SEQ ID NO.33), preferably an amino acid sequence having at least 70% (e.g., at least 70%, 71%, 72%, 73%, 74, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100%) sequence identity to the amino acid sequence of SEQ ID NO.1.

[0051] Item 3. A method for modifying at least one trichothene, preferably 3-keto-deoxynivalenol (3-keto-DON), for example detoxifying it, the method comprising forming a reaction mixture by contacting at least one polypeptide with a nutrient composition comprising at least one trichothene and with NAD(H) and / or NADP(H), preferably with NAD(H), the at least one polypeptide comprising an amino acid sequence selected from SEQ ID NO. 34 to SEQ ID NO. 54 (such as SEQ ID NO. 34, SEQ ID NO. 35, SEQ ID NO. 36, SEQ ID NO. 37, SEQ ID NO. 38, SEQ ID NO. 39, SEQ ID NO. 40, SEQ ID NO. 41, SEQ ID NO. 42, SEQ ID NO. 43, SEQ ID NO. 44, SEQ ID NO. 45, SEQ ID NO. 46, SEQ ID NO. 47, SEQ ID NO. 48, SEQ ID NO. 49, SEQ ID NO. 50, SEQ ID NO. 51, SEQ ID NO. 52, SEQ ID NO. 53, SEQ ID NO. 54, SEQ ID NO. 34 to SEQ ID NO. 35, SEQ ID NO. 34 to SEQ ID NO. 36, SEQ ID NO. 34 to SEQ ID NO. 37, SEQ ID NO. 34 to SEQ ID NO. 38, SEQ ID NO. 34 to SEQ ID NO. 39, SEQ ID NO. 34 to SEQ ID NO. 40, SEQ ID NO. 34 to SEQ ID NO. 41, SEQ ID NO. 34 to SEQ ID NO. 42, SEQ ID NO. 34 to SEQ ID NO. 43, SEQ ID NO. 34 to SEQ ID NO. 44, SEQ ID NO. 34 to SEQ ID NO. 45, SEQ ID NO. 34 to SEQ ID NO. 46, SEQ ID NO. 34 to SEQ ID NO. 47, SEQ ID NO. 34 to SEQ ID NO. 48, SEQ ID NO. 34 to SEQ ID NO. 49, SEQ ID NO. 34 to SEQ ID NO. 50, SEQ ID NO. 34 to SEQ ID NO. 51, SEQ ID NO. 34 to SEQ ID NO. 52, SEQ ID NO. 34 to SEQ ID NO. 53, and / or SEQ ID NO. 34 to SEQ ID NO. 54), preferably with SEQ ID NO.An amino acid sequence having at least 70% (e.g., at least 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100%) sequence identity to the amino acid sequence of 34.

[0052] Item 4. The method according to any one of the preceding items, wherein the at least one polypeptide is capable of modifying trichothecene, preferably modifying trichothecene at the C3 atom.

[0053] Item 5. The method according to any one of the preceding items, wherein the at least one polypeptide is capable of modifying deoxynivalenol, preferably modifying deoxynivalenol at the C3 atom.

[0054] Item 6. The method according to any one of the preceding items, wherein the at least one polypeptide is capable of modifying 3-keto-deoxynivalenol, preferably modifying 3-keto-deoxynivalenol at the C3 atom.

[0055] Item 7. The method according to any one of the preceding items, wherein the method further comprises incubating the reaction mixture at a temperature of 5 - 40°C, preferably at a temperature of 20 - 40°C.

[0056] Item 8. The method according to any one of the preceding items, wherein the step of incubating the reaction mixture is carried out for at least 5 minutes, preferably at least 10 minutes.

[0057] Item 9. The method according to any one of Items 1, 2, 4, 5, 7, 8, wherein the at least one trichothene is deoxynivalenol, and wherein the at least one polypeptide is selected from SEQ ID NO.1 to SEQ ID NO.33 (such as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9, SEQ ID NO.10, SEQ ID NO.11, SEQ ID NO.12, SEQ ID NO.13, SEQ ID NO.14, SEQ ID NO.15, SEQ ID NO.16, SEQ ID NO.17, SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.21, SEQ ID NO.22, SEQ ID NO.23, SEQ ID NO.24, SEQ ID NO.25, SEQ ID NO.26, SEQ ID NO.27, SEQ ID NO.28, SEQ ID NO.29, SEQ ID NO.30, SEQ ID NO.31, SEQ ID NO.32, SEQ ID NO.33, SEQ ID NO.1 to SEQ ID NO.2, SEQ ID NO.1 to SEQ ID NO.3, SEQ ID NO.1 to SEQ ID NO.4, SEQ ID NO.1 to SEQ ID NO.5, SEQ ID NO.1 to SEQ ID NO.6, SEQ ID NO.1 to SEQ ID NO.7, SEQ ID NO.1 to SEQ ID NO.8, SEQ ID NO.1 to SEQ ID NO.9, SEQ ID NO.1 to SEQ ID NO.10, SEQ ID NO.1 to SEQ ID NO.11, SEQ ID NO.1 to SEQ ID NO.12, SEQ ID NO.1 to SEQ ID NO.13, SEQ ID NO.1 to SEQ ID NO.14, SEQ ID NO.1 to SEQ ID NO.15, SEQ ID NO.1 to SEQ ID NO.16, SEQ ID NO.1 to SEQ ID NO.17, SEQ ID NO.1 to SEQ ID NO.18, SEQ ID NO.1 to SEQ ID NO.19, SEQ ID NO.1 to SEQ ID NO.20, SEQ ID NO.1 to SEQ ID NO.The amino acid sequences of SEQ ID NO.1 to SEQ ID NO.22, SEQ ID NO.1 to SEQ ID NO.23, SEQ ID NO.1 to SEQ ID NO.24, SEQ ID NO.1 to SEQ ID NO.25, SEQ ID NO.1 to SEQ ID NO.26, SEQ ID NO.1 to SEQ ID NO.27, SEQ ID NO.1 to SEQ ID NO.28, SEQ ID NO.1 to SEQ ID NO.29, SEQ ID NO.1 to SEQ ID NO.30, SEQ ID NO.1 to SEQ ID NO.31, SEQ ID NO.1 to SEQ ID NO.32, and / or SEQ ID NO.1 to SEQ ID NO.33), preferably having at least 70% sequence identity with the amino acid sequence of SEQ ID NO.1.

[0058] Item 10. The method according to any one of Items 1, 3, 4, 6 - 8, wherein the at least one trichothene is 3 - keto - deoxynivalenol, and wherein the at least one polypeptide comprises an amino acid sequence selected from SEQ ID NO. 34 to SEQ ID NO. 54 (such as SEQ ID NO. 34, SEQ ID NO. 35, SEQ ID NO. 36, SEQ ID NO. 37, SEQ ID NO. 38, SEQ ID NO. 39, SEQ ID NO. 40, SEQ ID NO. 41, SEQ ID NO. 42, SEQ ID NO. 43, SEQ ID NO. 44, SEQ ID NO. 45, SEQ ID NO. 46, SEQ ID NO. 47, SEQ ID NO. 48, SEQ ID NO. 49, SEQ ID NO. 50, SEQ ID NO. 51, SEQ ID NO. 52, SEQ ID NO. 53, SEQ ID NO. 54, SEQ ID NO. 34 to SEQ ID NO. 35, SEQ ID NO. 34 to SEQ ID NO. 36, SEQ ID NO. 34 to SEQ ID NO. 37, SEQ ID NO. 34 to SEQ ID NO. 38, SEQ ID NO. 34 to SEQ ID NO. 39, SEQ ID NO. 34 to SEQ ID NO. 40, SEQ ID NO. 34 to SEQ ID NO. 41, SEQ ID NO. 34 to SEQ ID NO. 42, SEQ ID NO. 34 to SEQ ID NO. 43, SEQ ID NO. 34 to SEQ ID NO. 44, SEQ ID NO. 34 to SEQ ID NO. 45, SEQ ID NO. 34 to SEQ ID NO. 46, SEQ ID NO. 34 to SEQ ID NO. 47, SEQ ID NO. 34 to SEQ ID NO. 48, SEQ ID NO. 34 to SEQ ID NO. 49, SEQ ID NO. 34 to SEQ ID NO. 50, SEQ ID NO. 34 to SEQ ID NO. 51, SEQ ID NO. 34 to SEQ ID NO. 52, SEQ ID NO. 34 to SEQ ID NO. 53, and / or SEQ ID NO. 34 to SEQ ID NO. 54), preferably with SEQ ID NO.An amino acid sequence having at least 70% (such as at least 70%, 71%, 72%, 73%, 74, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100%) sequence identity to the amino acid sequence of 34.

[0059] Item 11. The method according to any one of Items 1, 7, and 8, wherein the first trichothene is deoxynivalenol, and wherein the first reaction mixture is formed by contacting a nutrient composition comprising deoxynivalenol with at least a first polypeptide, the at least first polypeptide comprising an amino acid sequence selected from SEQ ID NO.1 to SEQ ID NO.33 (such as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9, SEQ ID NO.10, SEQ ID NO.11, SEQ ID NO.12, SEQ ID NO.13, SEQ ID NO.14, SEQ ID NO.15, SEQ ID NO.16, SEQ ID NO.17, SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.21, SEQ ID NO.22, SEQ ID NO.23, SEQ ID NO.24, SEQ ID NO.25, SEQ ID NO.26, SEQ ID NO.27, SEQ ID NO.28, SEQ ID NO.29, SEQ ID NO.30, SEQ ID NO.31, SEQ ID NO.32, SEQ ID NO.33, SEQ ID NO.1 to SEQ ID NO.2, SEQ ID NO.1 to SEQ ID NO.3, SEQ ID NO.1 to SEQ ID NO.4, SEQ ID NO.1 to SEQ ID NO.5, SEQ ID NO.1 to SEQ ID NO.6, SEQ ID NO.1 to SEQ ID NO.7, SEQ ID NO.1 to SEQ ID NO.8, SEQ ID NO.1 to SEQ ID NO.9, SEQ ID NO.1 to SEQ ID NO.10, SEQ ID NO.1 to SEQ ID NO.11, SEQ ID NO.1 to SEQ ID NO.12, SEQ ID NO.1 to SEQ ID NO.13, SEQ ID NO.1 to SEQ ID NO.14, SEQ ID NO.1 to SEQ ID NO.15, SEQ ID NO.1 to SEQ ID NO.16, SEQ ID NO.1 to SEQ ID NO.17, SEQ ID NO.1 to SEQ ID NO.18, SEQ ID NO.1 to SEQ ID NO.19, SEQ ID NO.The amino acid sequence of 1 to SEQ ID NO.20, SEQ ID NO.1 to SEQ ID NO.21, SEQ ID NO.1 to SEQ ID NO.22, SEQ ID NO.1 to SEQ ID NO.23, SEQ ID NO.1 to SEQ ID NO.24, SEQ ID NO.1 to SEQ ID NO.25, SEQ ID NO.1 to SEQ ID NO.26, SEQ ID NO.1 to SEQ ID NO.27, SEQ ID NO.1 to SEQ ID NO.28, SEQ ID NO.1 to SEQ ID NO.29, SEQ ID NO.1 to SEQ ID NO.30, SEQ ID NO.1 to SEQ ID NO.31, SEQ ID NO.1 to SEQ ID NO.32, and / or SEQ ID NO.1 to SEQ ID NO.33), preferably an amino acid sequence having at least 70% (e.g., at least 70%, 71%, 72%, 73%, 74, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100%) sequence identity to the amino acid sequence of SEQ ID NO.1; wherein the second trichothecene is 3-keto-deoxynivalenol, and wherein the second reaction mixture is formed by contacting a nutrient composition comprising 3-keto-deoxynivalenol with at least a second peptide, said at least second peptide comprising an amino acid sequence selected from SEQ ID NO.34 to SEQ ID NO.54 (e.g., SEQ ID NO.34, SEQ ID NO.35, SEQ ID NO.36, SEQ ID NO.37, SEQ ID NO.38, SEQ ID NO.39, SEQ ID NO.40, SEQ ID NO.41, SEQ ID NO.42, SEQ ID NO.43, SEQ ID NO.44, SEQ ID NO.45, SEQ ID NO.46, SEQ ID NO.47, SEQ ID NO.48, SEQ ID NO.49, SEQ ID NO.50, SEQ ID NO.51, SEQ ID NO.52, SEQ ID NO.53, SEQ ID NO.54, SEQ ID NO.34 to SEQ ID NO.35, SEQ ID NO.34 to SEQ ID NO.36, SEQ ID NO.34 to SEQ ID NO.37, SEQ ID NO.34 to SEQ ID NO.38. The amino acid sequence of SEQ ID NO.34 to SEQ ID NO.39, SEQ ID NO.34 to SEQ ID NO.40, SEQ ID NO.34 to SEQ ID NO.41, SEQ ID NO.34 to SEQ ID NO.42, SEQ ID NO.34 to SEQ ID NO.43, SEQ ID NO.34 to SEQ ID NO.44, SEQ ID NO.34 to SEQ ID NO.45, SEQ ID NO.34 to SEQ ID NO.46, SEQ ID NO.34 to SEQ ID NO.47, SEQ ID NO.34 to SEQ ID NO.48, SEQ ID NO.34 to SEQ ID NO.49, SEQ ID NO.34 to SEQ ID NO.50, SEQ ID NO.34 to SEQ ID NO.51, SEQ ID NO.34 to SEQ ID NO.52, SEQ ID NO.34 to SEQ ID NO.53, and / or SEQ ID NO.34 to SEQ ID NO.54), preferably an amino acid sequence having at least 70% (such as at least 70%, 71%, 72%, 73%, 74, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100%) sequence identity with the amino acid sequence of SEQ ID NO.34.

[0060] Item 12. The method according to item 11, wherein the at least first polypeptide is capable of modifying deoxynivalenol, preferably modifying deoxynivalenol at the C3 atom, and wherein the at least second polypeptide is capable of modifying 3-keto-deoxynivalenol, preferably modifying 3-keto-deoxynivalenol at the C3 atom.

[0061] Item 13. A method for modifying at least one trichothecene, preferably deoxynivalenol (DON), such as detoxifying it, the method comprising

[0062] (i) forming 3-keto-deoxynivalenol (3-keto-DON) and NAD + , including contacting at least a first polypeptide with a nutrient composition comprising DON and with NAD +Contact, wherein the at least first polypeptide comprises an amino acid sequence selected from SEQ ID NO.1 to SEQ ID NO.33 (such as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9, SEQ ID NO.10, SEQ ID NO.11, SEQ ID NO.12, SEQ ID NO.13, SEQ ID NO.14, SEQ ID NO.15, SEQ ID NO.16, SEQ ID NO.17, SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.21, SEQ ID NO.22, SEQ ID NO.23, SEQ ID NO.24, SEQ ID NO.25, SEQ ID NO.26, SEQ ID NO.27, SEQ ID NO.28, SEQ ID NO.29, SEQ ID NO.30, SEQ ID NO.31, SEQ ID NO.32, SEQ ID NO.33, SEQ ID NO.1 to SEQ ID NO.2, SEQ ID NO.1 to SEQ ID NO.3, SEQ ID NO.1 to SEQ ID NO.4, SEQ ID NO.1 to SEQ ID NO.5, SEQ ID NO.1 to SEQ ID NO.6, SEQ ID NO.1 to SEQ ID NO.7, SEQ ID NO.1 to SEQ ID NO.8, SEQ ID NO.1 to SEQ ID NO.9, SEQ ID NO.1 to SEQ ID NO.10, SEQ ID NO.1 to SEQ ID NO.11, SEQ ID NO.1 to SEQ ID NO.12, SEQ ID NO.1 to SEQ ID NO.13, SEQ ID NO.1 to SEQ ID NO.14, SEQ ID NO.1 to SEQ ID NO.15, SEQ ID NO.1 to SEQ ID NO.16, SEQ ID NO.1 to SEQ ID NO.17, SEQ ID NO.1 to SEQ ID NO.18, SEQ ID NO.1 to SEQ ID NO.19, SEQ ID NO.1 to SEQ ID NO.20, SEQ ID NO.1 to SEQ ID NO.21, SEQ ID NO.1 to SEQ ID NO.22, SEQ ID NO.1 to SEQ ID NO.23, SEQ ID NO.The amino acid sequence of 1 to SEQ ID NO.24, SEQ ID NO.1 to SEQ ID NO.25, SEQ ID NO.1 to SEQ ID NO.26, SEQ ID NO.1 to SEQ ID NO.27, SEQ ID NO.1 to SEQ ID NO.28, SEQ ID NO.1 to SEQ ID NO.29, SEQ ID NO.1 to SEQ ID NO.30, SEQ ID NO.1 to SEQ ID NO.31, SEQ ID NO.1 to SEQ ID NO.32, and / or SEQ ID NO.1 to SEQ ID NO.33), preferably an amino acid sequence having at least 70% (such as at least 70%, 71%, 72%, 73%, 74, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100%) sequence identity with the amino acid sequence of SEQ ID NO.1;.

[0063] (ii) Form 3-epi-deoxynivalenol (3-epi-DON) and NADH, including contacting 3-keto-DON, preferably 3-keto-DON formed in (i), with at least a second polypeptide and with NADH, preferably with NADH formed in (ii), wherein the at least second polypeptide comprises an amino acid sequence selected from SEQ ID NO. 34 to SEQ ID NO. 54 (such as SEQ ID NO. 34, SEQ ID NO. 35, SEQ ID NO. 36, SEQ ID NO. 37, SEQ ID NO. 38, SEQ ID NO. 39, SEQ ID NO. 40, SEQ ID NO. 41, SEQ ID NO. 42, SEQ ID NO. 43, SEQ ID NO. 44, SEQ ID NO. 45, SEQ ID NO. 46, SEQ ID NO. 47, SEQ ID NO. 48, SEQ ID NO. 49, SEQ ID NO. 50, SEQ ID NO. 51, SEQ ID NO. 52, SEQ ID NO. 53, SEQ ID NO. 54, SEQ ID NO. 34 to SEQ ID NO. 35, SEQ ID NO. 34 to SEQ ID NO. 36, SEQ ID NO. 34 to SEQ ID NO. 37, SEQ ID NO. 34 to SEQ ID NO. 38, SEQ ID NO. 34 to SEQ ID NO. 39, SEQ ID NO. 34 to SEQ ID NO. 40, SEQ ID NO. 34 to SEQ ID NO. 41, SEQ ID NO. 34 to SEQ ID NO. 42, SEQ ID NO. 34 to SEQ ID NO. 43, SEQ ID NO. 34 to SEQ ID NO. 44, SEQ ID NO. 34 to SEQ ID NO. 45, SEQ ID NO. 34 to SEQ ID NO. 46, SEQ ID NO. 34 to SEQ ID NO. 47, SEQ ID NO. 34 to SEQ ID NO. 48, SEQ ID NO. 34 to SEQ ID NO. 49, SEQ ID NO. 34 to SEQ ID NO. 50, SEQ ID NO. 34 to SEQ ID NO. 51, SEQ ID NO. 34 to SEQ ID NO. 52, SEQ ID NO. 34 to SEQ ID NO. 53, and / or SEQ ID NO. 34 to SEQ ID NO. 54), preferably with SEQ ID NO.An amino acid sequence having at least 70% (e.g., at least 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100%) sequence identity to the amino acid sequence of 34.

[0064] Item 14. The method according to item 13, wherein the at least first polypeptide is capable of modifying deoxynivalenol, preferably modifying deoxynivalenol at the C3 atom, and wherein the at least second polypeptide is capable of modifying 3-keto-deoxynivalenol, preferably modifying 3-keto-deoxynivalenol at the C3 atom.

[0065] Item 15. The method according to any one of items 11 to 14, wherein (i) and (ii) are carried out at a temperature of 5 - 40°C, preferably at a temperature of 20 - 40°C.

[0066] Item 16. The method according to any one of items 11 to 15, wherein (i) and (ii) are each carried out for at least 5 minutes, preferably at least 10 minutes.

[0067] Article 17. A composition, such as an additive for food and / or feed; feed or food, the composition being for modifying at least one trichothecene, such as detoxifying at least one trichothecene, the composition comprising at least one polypeptide, the at least one polypeptide comprising an amino acid sequence selected from SEQ ID NO.1 to SEQ ID NO.54 (such as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9, SEQ ID NO.10, SEQ ID NO.11, SEQ ID NO.12, SEQ ID NO.13, SEQ ID NO.14, SEQ ID NO.15, SEQ ID NO.16, SEQ ID NO.17, SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.21, SEQ ID NO.22, SEQ ID NO.23, SEQ ID NO.24, SEQ ID NO.25, SEQ ID NO.26, SEQ ID NO.27, SEQ ID NO.28, SEQ ID NO.29, SEQ ID NO.30, SEQ ID NO.31, SEQ ID NO.32, SEQ ID NO.33, SEQ ID NO.34, SEQ ID NO.35, SEQ ID NO.36, SEQ ID NO.37, SEQ ID NO.38, SEQ ID NO.39, SEQ ID NO.40, SEQ ID NO.41, SEQ ID NO.42, SEQ ID NO.43, SEQ ID NO.44, SEQ ID NO.45, SEQ ID NO.46, SEQ ID NO.47, SEQ ID NO.48, SEQ ID NO.49, SEQ ID NO.50, SEQ ID NO.51, SEQ ID NO.52, SEQ ID NO.53, SEQ ID NO.54, SEQ ID NO.1 to SEQ ID NO.54, SEQ ID NO.1 to SEQ ID NO.2, SEQ ID NO.1 to SEQ ID NO.3, SEQ ID NO.1 to SEQ ID NO.4, SEQ ID NO.1 to SEQ ID NO.5, SEQ ID NO.1 to SEQ ID NO.6, SEQ ID NO.1 to SEQ ID NO.7, SEQ ID NO.1 to SEQ ID NO.8, SEQ ID NO.1 to SEQ ID NO.9, SEQ ID NO.1 to SEQ ID NO.10, SEQ ID NO.1 to SEQ ID NO.11, SEQ ID NO.1 to SEQ ID NO.12, SEQ ID NO.1 to SEQ ID NO.13, SEQ ID NO.1 to SEQ ID NO.14, SEQ ID NO.1 to SEQ ID NO.15, SEQ ID NO.1 to SEQ ID NO.16, SEQ ID NO.1 to SEQ ID NO.17, SEQ ID NO.1 to SEQ ID NO.18, SEQ ID NO.1 to SEQ ID NO.19, SEQ ID NO.1 to SEQ ID NO.20, SEQ ID NO.1 to SEQ ID NO.21, SEQ ID NO.1 to SEQ ID NO.22, SEQ ID NO.1 to SEQ ID NO.23, SEQ ID NO.1 to SEQ ID NO.24, SEQ ID NO.1 to SEQ ID NO.25, SEQ ID NO.1 to SEQ ID NO.26, SEQ ID NO.1 to SEQ ID NO.27, SEQ ID NO.1 to SEQ ID NO.28, SEQ ID NO.1 to SEQ ID NO.29, SEQ ID NO.1 to SEQ ID NO.30, SEQ ID NO.1 to SEQ ID NO.31, SEQ ID NO.1 to SEQ ID NO.32, SEQ ID NO.1 to SEQ ID NO.33, SEQ ID NO.34 to SEQ ID NO.35, SEQ ID NO.34 to SEQ ID NO.36, SEQ ID NO.34 to SEQ ID NO.37, SEQ ID NO.34 to SEQ ID NO.38, SEQ ID NO.34 to SEQ ID NO.39, SEQ ID NO.34 to SEQ ID NO.40, SEQ ID NO.34 to SEQ ID NO.41, SEQ ID NO.34 to SEQ ID NO.42, SEQ ID NO.34 to SEQ ID NO.43, SEQ ID NO.34 to SEQ ID NO.44, SEQ ID NO.34 to SEQ ID NO.45, SEQ ID NO.34 to SEQ ID NO.46, SEQ ID NO.34 to SEQ ID NO.47, SEQ ID NO.34 to SEQ ID NO.48, SEQ ID NO.34 to SEQ ID NO.49. An amino acid sequence selected from SEQ ID NO. 34 to SEQ ID NO. 50, SEQ ID NO. 34 to SEQ ID NO. 51, SEQ ID NO. 34 to SEQ ID NO. 52, SEQ ID NO. 34 to SEQ ID NO. 53, and / or SEQ ID NO. 34 to SEQ ID NO. 54), preferably an amino acid sequence selected from SEQ ID NO. 1 and SEQ ID NO. 34, having at least 70% (e.g., at least 70%, 71%, 72%, 73%, 74, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100%) sequence identity.

[0068] Item 18. The composition according to Item 17, wherein the composition comprises a cofactor selected from NAD(H) and NADP(H), preferably NAD(H).

[0069] Item 19. The composition according to Item 17 or 18, wherein the composition comprises: at least a first polypeptide and at least a second polypeptide, the at least first polypeptide comprising an amino acid sequence selected from SEQ ID NO.1 to SEQ ID NO.33 (e.g., SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9, SEQ ID NO.10, SEQ ID NO.11, SEQ ID NO.12, SEQ ID NO.13, SEQ ID NO.14, SEQ ID NO.15, SEQ ID NO.16, SEQ ID NO.17, SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.21, SEQ ID NO.22, SEQ ID NO.23, SEQ ID NO.24, SEQ ID NO.25, SEQ ID NO.26, SEQ ID NO.27, SEQ ID NO.28, SEQ ID NO.29, SEQ ID NO.30, SEQ ID NO.31, SEQ ID NO.32, SEQ ID NO.33, SEQ ID NO.1 to SEQ ID NO.2, SEQ ID NO.1 to SEQ ID NO.3, SEQ ID NO.1 to SEQ ID NO.4, SEQ ID NO.1 to SEQ ID NO.5, SEQ ID NO.1 to SEQ ID NO.6, SEQ ID NO.1 to SEQ ID NO.7, SEQ ID NO.1 to SEQ ID NO.8, SEQ ID NO.1 to SEQ ID NO.9, SEQ ID NO.1 to SEQ ID NO.10, SEQ ID NO.1 to SEQ ID NO.11, SEQ ID NO.1 to SEQ ID NO.12, SEQ ID NO.1 to SEQ ID NO.13, SEQ ID NO.1 to SEQ ID NO.14, SEQ ID NO.1 to SEQ ID NO.15, SEQ ID NO.1 to SEQ ID NO.16, SEQ ID NO.1 to SEQ ID NO.17, SEQ ID NO.1 to SEQ ID NO.18, SEQ ID NO.1 to SEQ ID NO.19, SEQ ID NO.1 to SEQ ID NO.20, SEQ ID NO.1 to SEQ ID NO.21, SEQ ID NO.at least one amino acid sequence of 1 to SEQ ID NO.22, SEQ ID NO.1 to SEQ ID NO.23, SEQ ID NO.1 to SEQ ID NO.24, SEQ ID NO.1 to SEQ ID NO.25, SEQ ID NO.1 to SEQ ID NO.26, SEQ ID NO.1 to SEQ ID NO.27, SEQ ID NO.1 to SEQ ID NO.28, SEQ ID NO.1 to SEQ ID NO.29, SEQ ID NO.1 to SEQ ID NO.30, SEQ ID NO.1 to SEQ ID NO.31, SEQ ID NO.1 to SEQ ID NO.32, and / or SEQ ID NO.1 to SEQ ID NO.33), preferably an amino acid sequence having at least 70% (e.g., at least 70%, 71%, 72%, 73%, 74, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100%) sequence identity with the amino acid sequence of SEQ ID NO.1; said at least second polypeptide comprises an amino acid sequence selected from SEQ ID NO.34 to SEQ ID NO.54 (e.g., SEQ ID NO.34, SEQ ID NO.35, SEQ ID NO.36, SEQ ID NO.37, SEQ ID NO.38, SEQ ID NO.39, SEQ ID NO.40, SEQ ID NO.41, SEQ ID NO.42, SEQ ID NO.43, SEQ ID NO.44, SEQ ID NO.45, SEQ ID NO.46, SEQ ID NO.47, SEQ ID NO.48, SEQ ID NO.49, SEQ ID NO.50, SEQ ID NO.51, SEQ ID NO.52, SEQ ID NO.53, SEQ ID NO.54, SEQ ID NO.34 to SEQ ID NO.35, SEQ ID NO.34 to SEQ ID NO.36, SEQ ID NO.34 to SEQ ID NO.37, SEQ ID NO.34 to SEQ ID NO.38, SEQ ID NO.34 to SEQ ID NO.39, SEQ ID NO.34 to SEQ ID NO.40, SEQ ID NO.34 to SEQ ID NO.41, SEQ ID NO.34 to SEQ ID NO.42, SEQ ID NO.at least one amino acid sequence of SEQ ID NO. 34 to SEQ ID NO. 43, SEQ ID NO. 34 to SEQ ID NO. 44, SEQ ID NO. 34 to SEQ ID NO. 45, SEQ ID NO. 34 to SEQ ID NO. 46, SEQ ID NO. 34 to SEQ ID NO. 47, SEQ ID NO. 34 to SEQ ID NO. 48, SEQ ID NO. 34 to SEQ ID NO. 49, SEQ ID NO. 34 to SEQ ID NO. 50, SEQ ID NO. 34 to SEQ ID NO. 51, SEQ ID NO. 34 to SEQ ID NO. 52, SEQ ID NO. 34 to SEQ ID NO. 53, and / or SEQ ID NO. 34 to SEQ ID NO. 54), preferably an amino acid sequence having at least 70% (e.g., at least 70%, 71%, 72%, 73%, 74, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100%) sequence identity with the amino acid sequence of SEQ ID NO. 34.

[0070] Item 20. The composition according to any one of Items 17 - 19, wherein the composition comprises at least one carrier, and the at least one carrier is preferably selected from bentonite, silica, and carbohydrates.

[0071] Item 21. The composition according to any one of Items 17 - 20, wherein the composition is a feed additive.

[0072] Item 22. Use of at least one polypeptide for modifying trichothecene, in particular for detoxifying trichothecene, wherein the at least one polypeptide comprises an amino acid sequence having at least 70% (e.g., at least 70%, 71%, 72%, 73%, 74, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100%) sequence identity with an amino acid sequence selected from SEQ ID NO. 1 to SEQ ID NO. 54, preferably selected from SEQ ID NO. 1 and SEQ ID NO. 34.

[0073] Use of at least one polypeptide for modifying a trichothecene, preferably DON, in particular for detoxifying it, wherein said at least one polypeptide comprises a sequence selected from SEQ ID NO.1 to SEQ ID NO.33 (such as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9, SEQ ID NO.10, SEQ ID NO.11, SEQ ID NO.12, SEQ ID NO.13, SEQ ID NO.14, SEQ ID NO.15, SEQ ID NO.16, SEQ ID NO.17, SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.21, SEQ ID NO.22, SEQ ID NO.23, SEQ ID NO.24, SEQ ID NO.25, SEQ ID NO.26, SEQ ID NO.27, SEQ ID NO.28, SEQ ID NO.29, SEQ ID NO.30, SEQ ID NO.31, SEQ ID NO.32, SEQ ID NO.33, SEQ ID NO.1 to SEQ ID NO.2, SEQ ID NO.1 to SEQ ID NO.3, SEQ ID NO.1 to SEQ ID NO.4, SEQ ID NO.1 to SEQ ID NO.5, SEQ ID NO.1 to SEQ ID NO.6, SEQ ID NO.1 to SEQ ID NO.7, SEQ ID NO.1 to SEQ ID NO.8, SEQ ID NO.1 to SEQ ID NO.9, SEQ ID NO.1 to SEQ ID NO.10, SEQ ID NO.1 to SEQ ID NO.11, SEQ ID NO.1 to SEQ ID NO.12, SEQ ID NO.1 to SEQ ID NO.13, SEQ ID NO.1 to SEQ ID NO.14, SEQ ID NO.1 to SEQ ID NO.15, SEQ ID NO.1 to SEQ ID NO.16, SEQ ID NO.1 to SEQ ID NO.17, SEQ ID NO.1 to SEQ ID NO.18, SEQ ID NO.1 to SEQ ID NO.19, SEQ ID NO.1 to SEQ ID NO.20, SEQ ID NO.1 to SEQ ID NO.21, SEQ ID NO.the amino acid sequence of 1 to SEQ ID NO.22, SEQ ID NO.1 to SEQ ID NO.23, SEQ ID NO.1 to SEQ ID NO.24, SEQ ID NO.1 to SEQ ID NO.25, SEQ ID NO.1 to SEQ ID NO.26, SEQ ID NO.1 to SEQ ID NO.27, SEQ ID NO.1 to SEQ ID NO.28, SEQ ID NO.1 to SEQ ID NO.29, SEQ ID NO.1 to SEQ ID NO.30, SEQ ID NO.1 to SEQ ID NO.31, SEQ ID NO.1 to SEQ ID NO.32, and / or SEQ ID NO.1 to SEQ ID NO.33), preferably an amino acid sequence having at least 70% (e.g., at least 70%, 71%, 72%, 73%, 74, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100%) sequence identity with the amino acid sequence of SEQ ID NO.1.

[0074] Use of at least one polypeptide for modifying a trichothecene, preferably 3-keto-DON, in particular for detoxifying it, wherein the at least one polypeptide comprises an amino acid sequence selected from SEQ ID NOs. 34 to 54 (such as SEQ ID NO. 34, SEQ ID NO. 35, SEQ ID NO. 36, SEQ ID NO. 37, SEQ ID NO. 38, SEQ ID NO. 39, SEQ ID NO. 40, SEQ ID NO. 41, SEQ ID NO. 42, SEQ ID NO. 43, SEQ ID NO. 44, SEQ ID NO. 45, SEQ ID NO. 46, SEQ ID NO. 47, SEQ ID NO. 48, SEQ ID NO. 49, SEQ ID NO. 50, SEQ ID NO. 51, SEQ ID NO. 52, SEQ ID NO. 53, SEQ ID NO. 54, SEQ ID NOs. 34 to 35, SEQ ID NOs. 34 to 36, SEQ ID NOs. 34 to 37, SEQ ID NOs. 34 to 38, SEQ ID NOs. 34 to 39, SEQ ID NOs. 34 to 40, SEQ ID NOs. 34 to 41, SEQ ID NOs. 34 to 42, SEQ ID NOs. 34 to 43, SEQ ID NOs. 34 to 44, SEQ ID NOs. 34 to 45, SEQ ID NOs. 34 to 46, SEQ ID NOs. 34 to 47, SEQ ID NOs. 34 to 48, SEQ ID NOs. 34 to 49, SEQ ID NOs. 34 to 50, SEQ ID NOs. 34 to 51, SEQ ID NOs. 34 to 52, SEQ ID NOs. 34 to 53, and / or SEQ ID NOs. 34 to 54), preferably with SEQ ID NO.An amino acid sequence having at least 70% (e.g., at least 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100%) sequence identity to the amino acid sequence of 34.

[0075] Use of at least two polypeptides for modifying trichothecenes, preferably DON, in particular for detoxifying same, wherein at least a first polypeptide comprises an amino acid sequence selected from SEQ ID NO.1 to SEQ ID NO.33 (such as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9, SEQ ID NO.10, SEQ ID NO.11, SEQ ID NO.12, SEQ ID NO.13, SEQ ID NO.14, SEQ ID NO.15, SEQ ID NO.16, SEQ ID NO.17, SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.21, SEQ ID NO.22, SEQ ID NO.23, SEQ ID NO.24, SEQ ID NO.25, SEQ ID NO.26, SEQ ID NO.27, SEQ ID NO.28, SEQ ID NO.29, SEQ ID NO.30, SEQ ID NO.31, SEQ ID NO.32, SEQ ID NO.33, SEQ ID NO.1 to SEQ ID NO.2, SEQ ID NO.1 to SEQ ID NO.3, SEQ ID NO.1 to SEQ ID NO.4, SEQ ID NO.1 to SEQ ID NO.5, SEQ ID NO.1 to SEQ ID NO.6, SEQ ID NO.1 to SEQ ID NO.7, SEQ ID NO.1 to SEQ ID NO.8, SEQ ID NO.1 to SEQ ID NO.9, SEQ ID NO.1 to SEQ ID NO.10, SEQ ID NO.1 to SEQ ID NO.11, SEQ ID NO.1 to SEQ ID NO.12, SEQ ID NO.1 to SEQ ID NO.13, SEQ ID NO.1 to SEQ ID NO.14, SEQ ID NO.1 to SEQ ID NO.15, SEQ ID NO.1 to SEQ ID NO.16, SEQ ID NO.1 to SEQ ID NO.17, SEQ ID NO.1 to SEQ ID NO.18, SEQ ID NO.1 to SEQ ID NO.19, SEQ ID NO.1 to SEQ ID NO.20, SEQ ID NO.1 to SEQ ID NO.21, SEQ ID NO.the amino acid sequence of 1 to SEQ ID NO.22, SEQ ID NO.1 to SEQ ID NO.23, SEQ ID NO.1 to SEQ ID NO.24, SEQ ID NO.1 to SEQ ID NO.25, SEQ ID NO.1 to SEQ ID NO.26, SEQ ID NO.1 to SEQ ID NO.27, SEQ ID NO.1 to SEQ ID NO.28, SEQ ID NO.1 to SEQ ID NO.29, SEQ ID NO.1 to SEQ ID NO.30, SEQ ID NO.1 to SEQ ID NO.31, SEQ ID NO.1 to SEQ ID NO.32, and / or SEQ ID NO.1 to SEQ ID NO.33), preferably an amino acid sequence having at least 70% (such as at least 70%, 71%, 72%, 73%, 74, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100%) sequence identity with the amino acid sequence of SEQ ID NO.1; and wherein at least the second polypeptide comprises an amino acid sequence selected from SEQ ID NO.34 to SEQ ID NO.54 (such as SEQ ID NO.34, SEQ ID NO.35, SEQ ID NO.36, SEQ ID NO.37, SEQ ID NO.38, SEQ ID NO.39, SEQ ID NO.40, SEQ ID NO.41, SEQ ID NO.42, SEQ ID NO.43, SEQ ID NO.44, SEQ ID NO.45, SEQ ID NO.46, SEQ ID NO.47, SEQ ID NO.48, SEQ ID NO.49, SEQ ID NO.50, SEQ ID NO.51, SEQ ID NO.52, SEQ ID NO.53, SEQ ID NO.54, SEQ ID NO.34 to SEQ ID NO.35, SEQ ID NO.34 to SEQ ID NO.36, SEQ ID NO.34 to SEQ ID NO.37, SEQ ID NO.34 to SEQ ID NO.38, SEQ ID NO.34 to SEQ ID NO.39, SEQ ID NO.34 to SEQ ID NO.40, SEQ ID NO.34 to SEQ ID NO.41, SEQ ID NO.34 to SEQ ID NO.42, SEQ ID NO.the amino acid sequence of SEQ ID NO. 34 to SEQ ID NO. 43, SEQ ID NO. 34 to SEQ ID NO. 44, SEQ ID NO. 34 to SEQ ID NO. 45, SEQ ID NO. 34 to SEQ ID NO. 46, SEQ ID NO. 34 to SEQ ID NO. 47, SEQ ID NO. 34 to SEQ ID NO. 48, SEQ ID NO. 34 to SEQ ID NO. 49, SEQ ID NO. 34 to SEQ ID NO. 50, SEQ ID NO. 34 to SEQ ID NO. 51, SEQ ID NO. 34 to SEQ ID NO. 52, SEQ ID NO. 34 to SEQ ID NO. 53, and / or SEQ ID NO. 34 to SEQ ID NO. 54), preferably an amino acid sequence having at least 70% (such as at least 70%, 71%, 72%, 73%, 74, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100%) sequence identity with the amino acid sequence of SEQ ID NO. 34.

[0076] Item 25. The use according to Item 24, wherein the at least first polypeptide is capable of modifying DON, in particular detoxifying DON, and wherein the at least second polypeptide is capable of modifying 3-keto-DON, in particular detoxifying 3-keto-DON.

[0077] Item 26. The use of at least one polypeptide for manufacturing an additive for feed and / or food, a feed and / or food composition, or a pharmaceutical composition, wherein the at least one polypeptide comprises an amino acid sequence having at least 70% (such as at least 70%, 71%, 72%, 73%, 74, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100%) sequence identity with an amino acid sequence selected from SEQ ID NO. 1 to SEQ ID NO. 54, preferably selected from SEQ ID NO. 1 and SEQ ID NO. 34.

[0078] Use of at least one polypeptide for the production of biogas; bioethanol, DDGS; sugar, preferably sugar from sugar cane or sugar beet; corn oil, corn germ, corn germ meal, corn fiber, corn gluten, starch, in particular corn starch; and / or silage; wherein the at least one polypeptide comprises an amino acid sequence having at least 70% (such as at least 70%, 71%, 72%, 73%, 74, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100%) sequence identity with an amino acid sequence selected from SEQ ID NO.1 to SEQ ID NO.54, preferably selected from SEQ ID NO.1 and SEQ ID NO.34.

[0079] Item 28 The use according to any one of Items 26-27, wherein the at least one polypeptide comprises a sequence selected from SEQ ID NO.1 to SEQ ID NO.33 (such as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9, SEQ ID NO.10, SEQ ID NO.11, SEQ ID NO.12, SEQ ID NO.13, SEQ ID NO.14, SEQ ID NO.15, SEQ ID NO.16, SEQ ID NO.17, SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.21, SEQ ID NO.22, SEQ ID NO.23, SEQ ID NO.24, SEQ ID NO.25, SEQ ID NO.26, SEQ ID NO.27, SEQ ID NO.28, SEQ ID NO.29, SEQ ID NO.30, SEQ ID NO.31, SEQ ID NO.32, SEQ ID NO.33, SEQ ID NO.1 to SEQ ID NO.2, SEQ ID NO.1 to SEQ ID NO.3, SEQ ID NO.1 to SEQ ID NO.4, SEQ ID NO.1 to SEQ ID NO.5, SEQ ID NO.1 to SEQ ID NO.6, SEQ ID NO.1 to SEQ ID NO.7, SEQ ID NO.1 to SEQ ID NO.8, SEQ ID NO.1 to SEQ ID NO.9, SEQ ID NO.1 to SEQ ID NO.10, SEQ ID NO.1 to SEQ ID NO.11, SEQ ID NO.1 to SEQ ID NO.12, SEQ ID NO.1 to SEQ ID NO.13, SEQ ID NO.1 to SEQ ID NO.14, SEQ ID NO.1 to SEQ ID NO.15, SEQ ID NO.1 to SEQ ID NO.16, SEQ ID NO.1 to SEQ ID NO.17, SEQ ID NO.1 to SEQ ID NO.18, SEQ ID NO.1 to SEQ ID NO.19, SEQ ID NO.1 to SEQ ID NO.20, SEQ ID NO.1 to SEQ ID NO.21, SEQ ID NO.1 to SEQ ID NO.22, SEQ ID NO.The amino acid sequence of 1 to SEQ ID NO.23, SEQ ID NO.1 to SEQ ID NO.24, SEQ ID NO.1 to SEQ ID NO.25, SEQ ID NO.1 to SEQ ID NO.26, SEQ ID NO.1 to SEQ ID NO.27, SEQ ID NO.1 to SEQ ID NO.28, SEQ ID NO.1 to SEQ ID NO.29, SEQ ID NO.1 to SEQ ID NO.30, SEQ ID NO.1 to SEQ ID NO.31, SEQ ID NO.1 to SEQ ID NO.32, and / or SEQ ID NO.1 to SEQ ID NO.33), preferably an amino acid sequence having at least 70% (e.g., at least 70%, 71%, 72%, 73%, 74, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100%) sequence identity with the amino acid sequence of SEQ ID NO.1..

[0080] Item 29. The use according to any one of Items 26 - 27, wherein the at least one polypeptide comprises an amino acid sequence selected from SEQ ID NOs. 34 to 54 (such as SEQ ID NO. 34, SEQ ID NO. 35, SEQ ID NO. 36, SEQ ID NO. 37, SEQ ID NO. 38, SEQ ID NO. 39, SEQ ID NO. 40, SEQ ID NO. 41, SEQ ID NO. 42, SEQ ID NO. 43, SEQ ID NO. 44, SEQ ID NO. 45, SEQ ID NO. 46, SEQ ID NO. 47, SEQ ID NO. 48, SEQ ID NO. 49, SEQ ID NO. 50, SEQ ID NO. 51, SEQ ID NO. 52, SEQ ID NO. 53, SEQ ID NO. 54, SEQ ID NOs. 34 to 35, SEQ ID NOs. 34 to 36, SEQ ID NOs. 34 to 37, SEQ ID NOs. 34 to 38, SEQ ID NOs. 34 to 39, SEQ ID NOs. 34 to 40, SEQ ID NOs. 34 to 41, SEQ ID NOs. 34 to 42, SEQ ID NOs. 34 to 43, SEQ ID NOs. 34 to 44, SEQ ID NOs. 34 to 45, SEQ ID NOs. 34 to 46, SEQ ID NOs. 34 to 47, SEQ ID NOs. 34 to 48, SEQ ID NOs. 34 to 49, SEQ ID NOs. 34 to 50, SEQ ID NOs. 34 to 51, SEQ ID NOs. 34 to 52, SEQ ID NOs. 34 to 53, and / or SEQ ID NOs. 34 to 54), preferably with SEQ ID NO.An amino acid sequence having at least 70% (such as at least 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100%) sequence identity to the amino acid sequence of 34.

[0081] Item 30 The use according to any one of Items 26-27, wherein at least the first polypeptide comprises a sequence selected from SEQ ID NO.1 to SEQ ID NO.33 (such as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9, SEQ ID NO.10, SEQ ID NO.11, SEQ ID NO.12, SEQ ID NO.13, SEQ ID NO.14, SEQ ID NO.15, SEQ ID NO.16, SEQ ID NO.17, SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.21, SEQ ID NO.22, SEQ ID NO.23, SEQ ID NO.24, SEQ ID NO.25, SEQ ID NO.26, SEQ ID NO.27, SEQ ID NO.28, SEQ ID NO.29, SEQ ID NO.30, SEQ ID NO.31, SEQ ID NO.32, SEQ ID NO.33, SEQ ID NO.1 to SEQ ID NO.2, SEQ ID NO.1 to SEQ ID NO.3, SEQ ID NO.1 to SEQ ID NO.4, SEQ ID NO.1 to SEQ ID NO.5, SEQ ID NO.1 to SEQ ID NO.6, SEQ ID NO.1 to SEQ ID NO.7, SEQ ID NO.1 to SEQ ID NO.8, SEQ ID NO.1 to SEQ ID NO.9, SEQ ID NO.1 to SEQ ID NO.10, SEQ ID NO.1 to SEQ ID NO.11, SEQ ID NO.1 to SEQ ID NO.12, SEQ ID NO.1 to SEQ ID NO.13, SEQ ID NO.1 to SEQ ID NO.14, SEQ ID NO.1 to SEQ ID NO.15, SEQ ID NO.1 to SEQ ID NO.16, SEQ ID NO.1 to SEQ ID NO.17, SEQ ID NO.1 to SEQ ID NO.18, SEQ ID NO.1 to SEQ ID NO.19, SEQ ID NO.1 to SEQ ID NO.20, SEQ ID NO.1 to SEQ ID NO.21, SEQ ID NO.1 to SEQ ID NO.22, SEQ ID NO.the amino acid sequences of 1 to SEQ ID NO.23, SEQ ID NO.1 to SEQ ID NO.24, SEQ ID NO.1 to SEQ ID NO.25, SEQ ID NO.1 to SEQ ID NO.26, SEQ ID NO.1 to SEQ ID NO.27, SEQ ID NO.1 to SEQ ID NO.28, SEQ ID NO.1 to SEQ ID NO.29, SEQ ID NO.1 to SEQ ID NO.30, SEQ ID NO.1 to SEQ ID NO.31, SEQ ID NO.1 to SEQ ID NO.32, and / or SEQ ID NO.1 to SEQ ID NO.33), preferably an amino acid sequence having at least 70% (e.g., at least 70%, 71%, 72%, 73%, 74, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100%) sequence identity with the amino acid sequence of SEQ ID NO.1; and wherein at least the second polypeptide comprises an amino acid sequence selected from SEQ ID NO.34 to SEQ ID NO.54 (e.g., SEQ ID NO.34, SEQ ID NO.35, SEQ ID NO.36, SEQ ID NO.37, SEQ ID NO.38, SEQ ID NO.39, SEQ ID NO.40, SEQ ID NO.41, SEQ ID NO.42, SEQ ID NO.43, SEQ ID NO.44, SEQ ID NO.45, SEQ ID NO.46, SEQ ID NO.47, SEQ ID NO.48, SEQ ID NO.49, SEQ ID NO.50, SEQ ID NO.51, SEQ ID NO.52, SEQ ID NO.53, SEQ ID NO.54, SEQ ID NO.34 to SEQ ID NO.35, SEQ ID NO.34 to SEQ ID NO.36, SEQ ID NO.34 to SEQ ID NO.37, SEQ ID NO.34 to SEQ ID NO.38, SEQ ID NO.34 to SEQ ID NO.39, SEQ ID NO.34 to SEQ ID NO.40, SEQ ID NO.34 to SEQ ID NO.41, SEQ ID NO.34 to SEQ ID NO.42, SEQ ID NO.34 to SEQ ID NO.43, SEQ ID NO.the amino acid sequence of SEQ ID NO. 34 to SEQ ID NO. 44, SEQ ID NO. 34 to SEQ ID NO. 45, SEQ ID NO. 34 to SEQ ID NO. 46, SEQ ID NO. 34 to SEQ ID NO. 47, SEQ ID NO. 34 to SEQ ID NO. 48, SEQ ID NO. 34 to SEQ ID NO. 49, SEQ ID NO. 34 to SEQ ID NO. 50, SEQ ID NO. 34 to SEQ ID NO. 51, SEQ ID NO. 34 to SEQ ID NO. 52, SEQ ID NO. 34 to SEQ ID NO. 53, and / or SEQ ID NO. 34 to SEQ ID NO. 54), preferably an amino acid sequence having at least 70% (such as at least 70%, 71%, 72%, 73%, 74, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100%) sequence identity to the amino acid sequence of SEQ ID NO. 34.

[0082] Item 31. A host cell comprising at least one polypeptide and / or at least one polynucleotide encoding said at least one polypeptide, wherein said at least one polypeptide comprises an amino acid sequence having at least 70% (such as at least 70%, 71%, 72%, 73%, 74, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100%) sequence identity to an amino acid sequence selected from SEQ ID NO. 1 to SEQ ID NO. 54, preferably selected from SEQ ID NO. 1 and SEQ ID NO. 34.

[0083] Entry 32. A polypeptide for modifying at least one trichothecene, preferably DON and / or 3-keto-DON, for example for detoxifying it, said polypeptide comprising an amino acid sequence selected from SEQ ID NO.1 to SEQ ID NO.22 (such as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9, SEQ ID NO.10, SEQ ID NO.11, SEQ ID NO.12, SEQ ID NO.13, SEQ ID NO.14, SEQ ID NO.15, SEQ ID NO.16, SEQ ID NO.17, SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.21, SEQ ID NO.22, SEQ ID NO.1 to SEQ ID NO.2, SEQ ID NO.1 to SEQ ID NO.3, SEQ ID NO.1 to SEQ ID NO.4, SEQ ID NO.1 to SEQ ID NO.5, SEQ ID NO.1 to SEQ ID NO.6, SEQ ID NO.1 to SEQ ID NO.7, SEQ ID NO.1 to SEQ ID NO.8, SEQ ID NO.1 to SEQ ID NO.9, SEQ ID NO.1 to SEQ ID NO.10, SEQ ID NO.1 to SEQ ID NO.11, SEQ ID NO.1 to SEQ ID NO.12, SEQ ID NO.1 to SEQ ID NO.13, SEQ ID NO.1 to SEQ ID NO.14, SEQ ID NO.1 to SEQ ID NO.15, SEQ ID NO.1 to SEQ ID NO.16, SEQ ID NO.1 to SEQ ID NO.17, SEQ ID NO.1 to SEQ ID NO.18, SEQ ID NO.1 to SEQ ID NO.19, SEQ ID NO.1 to SEQ ID NO.20, SEQ ID NO.1 to SEQ ID NO.21, and / or SEQ ID NO.1 to SEQ ID NO.22), preferably having at least 96.0% sequence identity with SEQ ID NO.1; or selected from SEQ ID NO.34 to SEQ ID NO.54 (such as SEQ ID NO.34, SEQ ID NO.35, SEQ ID NO.36, SEQ ID NO.The amino acid sequence of SEQ ID NO.37, SEQ ID NO.38, SEQ ID NO.39, SEQ ID NO.40, SEQ ID NO.41, SEQ ID NO.42, SEQ ID NO.43, SEQ ID NO.44, SEQ ID NO.45, SEQ ID NO.46, SEQ ID NO.47, SEQ ID NO.48, SEQ ID NO.49, SEQ ID NO.50, SEQ ID NO.51, SEQ ID NO.52, SEQ ID NO.53, SEQ ID NO.54, SEQ ID NO.34 to SEQ ID NO.35, SEQ ID NO.34 to SEQ ID NO.36, SEQ ID NO.34 to SEQ ID NO.37, SEQ ID NO.34 to SEQ ID NO.38, SEQ ID NO.34 to SEQ ID NO.39, SEQ ID NO.34 to SEQ ID NO.40, SEQ ID NO.34 to SEQ ID NO.41, SEQ ID NO.34 to SEQ ID NO.42, SEQ ID NO.34 to SEQ ID NO.43, SEQ ID NO.34 to SEQ ID NO.44, SEQ ID NO.34 to SEQ ID NO.45, SEQ ID NO.34 to SEQ ID NO.46, SEQ ID NO.34 to SEQ ID NO.47, SEQ ID NO.34 to SEQ ID NO.48, SEQ ID NO.34 to SEQ ID NO.49, SEQ ID NO.34 to SEQ ID NO.50, SEQ ID NO.34 to SEQ ID NO.51, SEQ ID NO.34 to SEQ ID NO.52, SEQ ID NO.34 to SEQ ID NO.53, and / or SEQ ID NO.34 to SEQ ID NO.54), preferably an amino acid sequence having at least 91.0% sequence identity with SEQ ID NO.34.

[0084] Entry 33. A polypeptide for modifying at least one trichothecene, preferably DON, for example, detoxifying it, said polypeptide comprising an amino acid sequence selected from SEQ ID NO.1 to SEQ ID NO.22 (such as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9, SEQ ID NO.10, SEQ ID NO.11, SEQ ID NO.12, SEQ ID NO.13, SEQ ID NO.14, SEQ ID NO.15, SEQ ID NO.16, SEQ ID NO.17, SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.21, SEQ ID NO.22, SEQ ID NO.1 to SEQ ID NO.2, SEQ ID NO.1 to SEQ ID NO.3, SEQ ID NO.1 to SEQ ID NO.4, SEQ ID NO.1 to SEQ ID NO.5, SEQ ID NO.1 to SEQ ID NO.6, SEQ ID NO.1 to SEQ ID NO.7, SEQ ID NO.1 to SEQ ID NO.8, SEQ ID NO.1 to SEQ ID NO.9, SEQ ID NO.1 to SEQ ID NO.10, SEQ ID NO.1 to SEQ ID NO.11, SEQ ID NO.1 to SEQ ID NO.12, SEQ ID NO.1 to SEQ ID NO.13, SEQ ID NO.1 to SEQ ID NO.14, SEQ ID NO.1 to SEQ ID NO.15, SEQ ID NO.1 to SEQ ID NO.16, SEQ ID NO.1 to SEQ ID NO.17, SEQ ID NO.1 to SEQ ID NO.18, SEQ ID NO.1 to SEQ ID NO.19, SEQ ID NO.1 to SEQ ID NO.20, SEQ ID NO.1 to SEQ ID NO.21, and / or SEQ ID NO.1 to SEQ ID NO.22), preferably an amino acid sequence having at least 96.0% sequence identity with SEQ ID NO.1.

[0085] Item 34. A polypeptide for modifying at least one trichothecene, preferably 3-keto-DON, for example, for detoxifying the same, said polypeptide comprising an amino acid sequence selected from SEQ ID NO. 34 to SEQ ID NO. 54 (such as SEQ ID NO. 34, SEQ ID NO. 35, SEQ ID NO. 36, SEQ ID NO. 37, SEQ ID NO. 38, SEQ ID NO. 39, SEQ ID NO. 40, SEQ ID NO. 41, SEQ ID NO. 42, SEQ ID NO. 43, SEQ ID NO. 44, SEQ ID NO. 45, SEQ ID NO. 46, SEQ ID NO. 47, SEQ ID NO. 48, SEQ ID NO. 49, SEQ ID NO. 50, SEQ ID NO. 51, SEQ ID NO. 52, SEQ ID NO. 53, SEQ ID NO. 54, SEQ ID NO. 34 to SEQ ID NO. 35, SEQ ID NO. 34 to SEQ ID NO. 36, SEQ ID NO. 34 to SEQ ID NO. 37, SEQ ID NO. 34 to SEQ ID NO. 38, SEQ ID NO. 34 to SEQ ID NO. 39, SEQ ID NO. 34 to SEQ ID NO. 40, SEQ ID NO. 34 to SEQ ID NO. 41, SEQ ID NO. 34 to SEQ ID NO. 42, SEQ ID NO. 34 to SEQ ID NO. 43, SEQ ID NO. 34 to SEQ ID NO. 44, SEQ ID NO. 34 to SEQ ID NO. 45, SEQ ID NO. 34 to SEQ ID NO. 46, SEQ ID NO. 34 to SEQ ID NO. 47, SEQ ID NO. 34 to SEQ ID NO. 48, SEQ ID NO. 34 to SEQ ID NO. 49, SEQ ID NO. 34 to SEQ ID NO. 50, SEQ ID NO. 34 to SEQ ID NO. 51, SEQ ID NO. 34 to SEQ ID NO. 52, SEQ ID NO. 34 to SEQ ID NO. 53, and / or SEQ ID NO. 34 to SEQ ID NO. 54), preferably an amino acid sequence having at least 91.0% sequence identity with SEQ ID NO. 34.

[0086] Item 35. A composition comprising at least two polypeptides, wherein at least the first polypeptide comprises an amino acid sequence having at least 96.0% (such as at least 96%, 97%, 98%, 99%, 100%) sequence identity with an amino acid sequence selected from SEQ ID NO.1 to SEQ ID NO.22, preferably with SEQ ID NO.1; and wherein at least the second polypeptide comprises an amino acid sequence having at least 91.0% (such as at least 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100%) sequence identity with an amino acid sequence selected from SEQ ID NO.34 to SEQ ID NO.54, preferably with SEQ ID NO.34.

[0087] Item 36. A polypeptide for treating, ameliorating and / or preventing symptoms caused by mycotoxin poisoning, especially trichothecene mycotoxin poisoning, wherein the polypeptide comprises an amino acid sequence having at least 70% (such as at least 70%, 71%, 72%, 73%, 74,75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100%) sequence identity with an amino acid sequence selected from SEQ ID NO.1 to SEQ ID NO.54, preferably selected from SEQ ID NO.1 and SEQ ID NO.34.

[0088] Item 37. A polypeptide for treating, ameliorating, and / or preventing symptoms caused by mycotoxin poisoning according to Item 36, wherein the polypeptide comprises a sequence selected from SEQ ID NO.1 to SEQ ID NO.33 (such as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9, SEQ ID NO.10, SEQ ID NO.11, SEQ ID NO.12, SEQ ID NO.13, SEQ ID NO.14, SEQ ID NO.15, SEQ ID NO.16, SEQ ID NO.17, SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.21, SEQ ID NO.22, SEQ ID NO.23, SEQ ID NO.24, SEQ ID NO.25, SEQ ID NO.26, SEQ ID NO.27, SEQ ID NO.28, SEQ ID NO.29, SEQ ID NO.30, SEQ ID NO.31, SEQ ID NO.32, SEQ ID NO.33, SEQ ID NO.1 to SEQ ID NO.2, SEQ ID NO.1 to SEQ ID NO.3, SEQ ID NO.1 to SEQ ID NO.4, SEQ ID NO.1 to SEQ ID NO.5, SEQ ID NO.1 to SEQ ID NO.6, SEQ ID NO.1 to SEQ ID NO.7, SEQ ID NO.1 to SEQ ID NO.8, SEQ ID NO.1 to SEQ ID NO.9, SEQ ID NO.1 to SEQ ID NO.10, SEQ ID NO.1 to SEQ ID NO.11, SEQ ID NO.1 to SEQ ID NO.12, SEQ ID NO.1 to SEQ ID NO.13, SEQ ID NO.1 to SEQ ID NO.14, SEQ ID NO.1 to SEQ ID NO.15, SEQ ID NO.1 to SEQ ID NO.16, SEQ ID NO.1 to SEQ ID NO.17, SEQ ID NO.1 to SEQ ID NO.18, SEQ ID NO.1 to SEQ ID NO.19, SEQ ID NO.1 to SEQ ID NO.20, SEQ ID NO.1 to SEQ ID NO.21, SEQ ID NO.The amino acid sequence of 1 to SEQ ID NO.22, SEQ ID NO.1 to SEQ ID NO.23, SEQ ID NO.1 to SEQ ID NO.24, SEQ ID NO.1 to SEQ ID NO.25, SEQ ID NO.1 to SEQ ID NO.26, SEQ ID NO.1 to SEQ ID NO.27, SEQ ID NO.1 to SEQ ID NO.28, SEQ ID NO.1 to SEQ ID NO.29, SEQ ID NO.1 to SEQ ID NO.30, SEQ ID NO.1 to SEQ ID NO.31, SEQ ID NO.1 to SEQ ID NO.32, and / or SEQ ID NO.1 to SEQ ID NO.33), preferably an amino acid sequence having at least 70% (such as at least 70%, 71%, 72%, 73%, 74, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100%) sequence identity with the amino acid sequence of SEQ ID NO.1.

[0089] Item 37. A polypeptide for treating, ameliorating, and / or preventing symptoms caused by mycotoxin poisoning according to Item 36, wherein the polypeptide comprises an amino acid sequence selected from SEQ ID NOs. 34 to 54 (such as SEQ ID NO. 34, SEQ ID NO. 35, SEQ ID NO. 36, SEQ ID NO. 37, SEQ ID NO. 38, SEQ ID NO. 39, SEQ ID NO. 40, SEQ ID NO. 41, SEQ ID NO. 42, SEQ ID NO. 43, SEQ ID NO. 44, SEQ ID NO. 45, SEQ ID NO. 46, SEQ ID NO. 47, SEQ ID NO. 48, SEQ ID NO. 49, SEQ ID NO. 50, SEQ ID NO. 51, SEQ ID NO. 52, SEQ ID NO. 53, SEQ ID NO. 54, SEQ ID NOs. 34 to 35, SEQ ID NOs. 34 to 36, SEQ ID NOs. 34 to 37, SEQ ID NOs. 34 to 38, SEQ ID NOs. 34 to 39, SEQ ID NOs. 34 to 40, SEQ ID NOs. 34 to 41, SEQ ID NOs. 34 to 42, SEQ ID NOs. 34 to 43, SEQ ID NOs. 34 to 44, SEQ ID NOs. 34 to 45, SEQ ID NOs. 34 to 46, SEQ ID NOs. 34 to 47, SEQ ID NOs. 34 to 48, SEQ ID NOs. 34 to 49, SEQ ID NOs. 34 to 50, SEQ ID NOs. 34 to 51, SEQ ID NOs. 34 to 52, SEQ ID NOs. 34 to 53, and / or SEQ ID NOs. 34 to 54), preferably to SEQ ID NO.An amino acid sequence having at least 70% (e.g., at least 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100%) sequence identity to the amino acid sequence of 34.

[0090] Item 38. A polypeptide composition for treating, ameliorating, and / or preventing symptoms caused by mycotoxin poisoning, wherein at least a first polypeptide comprises an amino acid sequence selected from SEQ ID NO.1 to SEQ ID NO.33 (such as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7, SEQ ID NO.8, SEQ ID NO.9, SEQ ID NO.10, SEQ ID NO.11, SEQ ID NO.12, SEQ ID NO.13, SEQ ID NO.14, SEQ ID NO.15, SEQ ID NO.16, SEQ ID NO.17, SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.21, SEQ ID NO.22, SEQ ID NO.23, SEQ ID NO.24, SEQ ID NO.25, SEQ ID NO.26, SEQ ID NO.27, SEQ ID NO.28, SEQ ID NO.29, SEQ ID NO.30, SEQ ID NO.31, SEQ ID NO.32, SEQ ID NO.33, SEQ ID NO.1 to SEQ ID NO.2, SEQ ID NO.1 to SEQ ID NO.3, SEQ ID NO.1 to SEQ ID NO.4, SEQ ID NO.1 to SEQ ID NO.5, SEQ ID NO.1 to SEQ ID NO.6, SEQ ID NO.1 to SEQ ID NO.7, SEQ ID NO.1 to SEQ ID NO.8, SEQ ID NO.1 to SEQ ID NO.9, SEQ ID NO.1 to SEQ ID NO.10, SEQ ID NO.1 to SEQ ID NO.11, SEQ ID NO.1 to SEQ ID NO.12, SEQ ID NO.1 to SEQ ID NO.13, SEQ ID NO.1 to SEQ ID NO.14, SEQ ID NO.1 to SEQ ID NO.15, SEQ ID NO.1 to SEQ ID NO.16, SEQ ID NO.1 to SEQ ID NO.17, SEQ ID NO.1 to SEQ ID NO.18, SEQ ID NO.1 to SEQ ID NO.19, SEQ ID NO.1 to SEQ ID NO.20, SEQ ID NO.1 to SEQ ID NO.21, SEQ ID NO.1 to SEQ ID NO.22. The amino acid sequences of SEQ ID NO.1 to SEQ ID NO.23, SEQ ID NO.1 to SEQ ID NO.24, SEQ ID NO.1 to SEQ ID NO.25, SEQ ID NO.1 to SEQ ID NO.26, SEQ ID NO.1 to SEQ ID NO.27, SEQ ID NO.1 to SEQ ID NO.28, SEQ ID NO.1 to SEQ ID NO.29, SEQ ID NO.1 to SEQ ID NO.30, SEQ ID NO.1 to SEQ ID NO.31, SEQ ID NO.1 to SEQ ID NO.32, and / or SEQ ID NO.1 to SEQ ID NO.33), preferably amino acid sequences having at least 70% (e.g., at least 70%, 71%, 72%, 73%, 74, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100%) sequence identity with the amino acid sequence of SEQ ID NO.1; and wherein at least the second polypeptide comprises an amino acid sequence selected from SEQ ID NO.34 to SEQ ID NO.54 (e.g., SEQ ID NO.34, SEQ ID NO.35, SEQ ID NO.36, SEQ ID NO.37, SEQ ID NO.38, SEQ ID NO.39, SEQ ID NO.40, SEQ ID NO.41, SEQ ID NO.42, SEQ ID NO.43, SEQ ID NO.44, SEQ ID NO.45, SEQ ID NO.46, SEQ ID NO.47, SEQ ID NO.48, SEQ ID NO.49, SEQ ID NO.50, SEQ ID NO.51, SEQ ID NO.52, SEQ ID NO.53, SEQ ID NO.54, SEQ ID NO.34 to SEQ ID NO.35, SEQ ID NO.34 to SEQ ID NO.36, SEQ ID NO.34 to SEQ ID NO.37, SEQ ID NO.34 to SEQ ID NO.38, SEQ ID NO.34 to SEQ ID NO.39, SEQ ID NO.34 to SEQ ID NO.40, SEQ ID NO.34 to SEQ ID NO.41, SEQ ID NO.34 to SEQ ID NO.42, SEQ ID NO.34 to SEQ ID NO.43. The amino acid sequence of SEQ ID NO.34 to SEQ ID NO.44, SEQ ID NO.34 to SEQ ID NO.45, SEQ ID NO.34 to SEQ ID NO.46, SEQ ID NO.34 to SEQ ID NO.47, SEQ ID NO.34 to SEQ ID NO.48, SEQ ID NO.34 to SEQ ID NO.49, SEQ ID NO.34 to SEQ ID NO.50, SEQ ID NO.34 to SEQ ID NO.51, SEQ ID NO.34 to SEQ ID NO.52, SEQ ID NO.34 to SEQ ID NO.53, and / or SEQ ID NO.34 to SEQ ID NO.54), preferably an amino acid sequence having at least 70% (such as at least 70%, 71%, 72%, 73%, 74, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 100%) sequence identity with the amino acid sequence of SEQ ID NO.34. Example

[0091] The present invention is further described below by non-limiting examples. The present invention as disclosed herein is not limited to the specific embodiments, methods, examples, schemes, etc. described herein, but is defined only by the claims.

[0092] Example 1

[0093] For the expression and protein purification of candidate genes, the nucleotide sequences encoding polypeptides having any of the amino acid sequences of SEQ ID NO.1 to SEQ ID NO.54 were cloned into plasmids for expression in a suitable host organism, particularly Escherichia coli. The recombinant vectors were based on the Escherichia coli expression vector pET28a, the nucleotide sequence of which contains the restriction site Ncol at the 5' end and the restriction site Xhol at the 3' end, as well as a 6xHis tag at the C-terminus or N-terminus to facilitate affinity purification. The untagged enzymes were produced in a similar manner and were found to have substantially the same enzymatic activity and protein properties.

[0094] Escherichia coli BL21(DE3) was transformed with a recombinant expression vector based on pET28a and induced for expression by adding IPTG. Cells were harvested by centrifugation and lysed by treatment with sonication or a French press. Finally, any method known to those skilled in the art suitable for generating a crude cell lysate from the host organism employed can be considered. Alternative recombinant protein production hosts can be considered, such as systems based on Bacillus, Pseudomonas, Pichia, Yarrowia, Streptomyces, Hansenula, Lactobacillus, Saccharomyces, Aspergillus, Trichoderma or other systems known in the art. The lysate was clarified by centrifugation and the supernatant containing the soluble recombinant protein was assayed to determine the catalytic properties of the enzyme produced. The target protein concentration was determined via SDS-PAGE by comparison with a bovine serum albumin (BSA, Sigma #A4919) protein standard at a concentration of 100 μg / mL to 1500 μg / mL BSA.

[0095] Example 2

[0096] To determine the applicability of the enzyme produced in Example 1 to modified trichothecene substrates, the enzyme was tested in an activity assay as described below.

[0097] The activity of the DON oxidase was tested in a standard DON degradation assay prepared as follows: 50 mM KPO4 buffer pH 5.4 - 8.0, 5 - 75 mg / L DON, 1.5 mM NAD(P) + , 2 mM MgCl2 and 1 μM enzyme from the cell lysate. In addition, an NAD(P)H scavenger or an NAD(P)H-consuming enzyme can be added to accelerate the reaction. Such an NAD(P)H scavenger can be a quinone at a concentration of 1 mM, such as pyrroloquinoline quinone (PQQ), or small benzoquinones and naphthoquinones, such as 1,4-benzoquinone, 2,6-dimethylbenzoquinone or 1,2-naphthoquinone. Alternatively, synthetic redox cofactors such as phenazine methosulfate (PMS), methylene blue, coenzyme Q10 and Q1 or potassium ferricyanide (III) can be used as NAD(P)H scavengers. As an alternative or supplement, an enzyme that recycles NAD(P) during NAD(P)H consumption can be added to the reaction. Surprisingly and advantageously, it was found that an enzyme having any of the amino acid sequences of SEQ.ID NO.34 to SEQ.IDNO.54 was capable of reducing NAD(P)H to form NAD(P) + ​+ and simultaneously oxidizing 3-keto-DON to form 3-epi DON, which is a molecule less toxic than DON or 3-keto-DON. Specifically, it was found that an enzyme having any one of the amino acid sequences of SEQ.ID NO.34 to SEQ.ID NO.54 is capable of reducing NADH to form NAD + while oxidizing 3-keto-DON to form 3-epi-DON.

[0098] In fact, all reaction components except NAD(P)+ were mixed on ice and a sample at 0 minutes was taken from the mixture. Subsequently, the reaction mixture was immediately transferred to a heating block and the reaction was initiated by adding the cofactor (i.e., NAD + and / or NADP + ) and the reaction was carried out at 37 °C for 2 - 3 hours. Samples were taken at regular intervals to monitor the reaction progress and were immediately mixed with 1 + 1 (v / v) of 100% acetonitrile to stop the reaction. Subsequently, it was analyzed using the HPLC-MS / MS method described below.

[0099] Example 3

[0100] Test the activity of an enzyme having any one of the amino acid sequences of SEQ.ID NO.1 to SEQ.ID NO.22 in an assay using an expression culture lysate, which was adjusted to OD 0.1 of a culture producing any one of the enzymes of SEQ.ID NO.1 to SEQ.ID NO.22. The comparability of the expression levels was verified by SDS-PAGE analysis. To test the modification of DON to 3-keto-DON, these lysates were mixed with 37 mg / L DON, 1.5 mM NAD + , 2 mM MgCl2, 0.1 mg / mL BSA. To test the further modification of 3-keto-DON to 3-epi-DON, these reaction mixtures were also mixed with an expression culture lysate, which was adjusted to OD 15 of a culture producing an enzyme having the amino acid sequence of SEQ.ID NO.36 in 50 mM potassium phosphate buffer, pH 5.4. These final reaction mixtures were incubated at 37 °C and 100 rpm for 3 hours and the 3-epi-DON formation rate was determined, see Table 1.

[0101] Table 1: Modification of DON to 3-keto-DON to 3-epi-DON.

[0102]

[0103] To further investigate the ability of enzyme modification of DON, which contains an amino acid with at least 70% identity to the amino acid sequence of SEQ ID NO:1, as an exemplary trichothecene substrate, the expression culture lysate of any one of the enzymes producing SEQ.ID.23 to SEQ.ID.33 as described in Example 1 was adjusted to OD 0.1 and mixed with 25 mg / L DON, 1.5 mM NADP + , 1 mM PQQ in potassium phosphate buffer at pH 8. These reaction mixtures were incubated at 37 °C for 2 h, and then 3-keto-DON was formed, see Table 2.

[0104] Table 2: Modification of DON to 3-keto-DON.

[0105]

[0106] It should be noted that when the DON-degrading enzyme with any one of the amino acid sequences of SEQ ID NO.1 to SEQ ID NO.33 was compared with the enzyme called AKR18A1 provided by He et al. (2017. Sci Rep 7(1):9549), it was found that the latter degraded DON at least two times slower than any one of the former.

[0107] In addition, the ability of enzyme modification of 3-keto-DON, which contains an amino acid with at least 70% identity to the amino acid sequence of SEQ ID NO:34, as another exemplary trichothecene substrate was tested. For this purpose, the expression culture lysate of any one of the enzymes producing SEQ.ID.34 to SEQ.ID.54 as described in Example 1 was adjusted to OD 1.5 and mixed with 37 mg / L DON, 1.5 mM NAD + , 2 mM MgCl2, 0.1 mg / mL BSA in 50 mM potassium phosphate buffer at pH 6. The expression culture lysate of the enzyme producing SEQ ID NO.1 adjusted to OD 15 was further added in 50 mM potassium phosphate buffer at pH 6 to allow the conversion of available DON to form 3-keto-DON, thereby initiating the reaction from 3-keto-DON to 3-epi-DON by any one of the enzymes of SEQ ID NO.34 to SEQ ID NO.54. These reaction mixtures were incubated at 37 °C at 100 rpm for 3 h, and then 3-epi-DON was formed, see Table 3.

[0108] Table 3: Modification of 3-keto-DON to 3-epi-DON.

[0109]

[0110] Finally, it was found that all exemplary tested enzymes were able to modify at least one trichothecene mycotoxin. Specifically, any one of the enzymes having any of the amino acid sequences of SEQ.ID NO.1 to SEQ.ID NO.33 was found to be particularly good at modifying DON to form 3-keto-DON while reducing NADP + . Most notably, any one of the enzymes having any of the amino acid sequences of SEQ.ID NO.1 to SEQ.ID NO.22 was found to be particularly good at modifying DON to form 3-keto-DON while reducing NAD + , thus allowing the use of the more stable cofactor NAD + instead of NADP + . Further, any one of the enzymes having any of the amino acid sequences of SEQ ID NO.34 to SEQ ID NO.54 was found to be particularly good at modifying 3-keto-DON to form 3-epi-DON while oxidizing NADH to form NAD + , thus facilitating cofactor recycling. Notably, it was found that, unlike the enzymes having any of the amino acid sequences of SEQ ID NO.34 to SEQ ID NO.54, the previously described enzymes capable of reducing 3-keto-DON to form DON (e.g., in WO 2021 / 038026 A1) were not suitable for recycling NADH to NAD + . The combination of any one of the enzymes having any of the amino acid sequences of SEQ ID NO.1 to SEQ ID NO.33 with any one of the enzymes having any of the amino acid sequences of SEQ ID NO.34 to SEQ ID NO.54 allows the formation of 3-epi-DON from DON via 3-keto-DON. Under the recycling of NAD(H) as a cofactor, a particularly effective formation of 3-epi-DON from DON via 3-keto-DON was identified in the combination of any one of the enzymes having any of the amino acid sequences of SEQ ID NO.1 to SEQ ID NO.22 with any one of the enzymes having any of the amino acid sequences of SEQ ID NO.34 to SEQ ID NO.54.

[0111] Example 4

[0112] For HPLC analysis, a 150 mm × 4.6 mm Phenomenex Gemini-NX C18 column with a particle size of 5 μm was used to separate the samples. A mixture of acetonitrile and ultrapure water was used as the mobile phase, and the ultraviolet (UV) signal at 220 nm was recorded and evaluated to determine the concentration of trichothecene analytes (i.e., DON, 3-keto-DON, and / or 3-epi-DON).

[0113] Alternatively or additionally, the samples can be analyzed by LC-MS / MS. For this purpose, DON and its metabolites were separated on a 150 mm × 2.1 mm Phenomenex Kinetex biphenyl column with a particle size of 2.6 μm. Before performing LC-MS / MS analysis, all samples were diluted with 50% aqueous acetonitrile to give a theoretical final concentration of 0.2 ppm of DON in the samples. The mobile phase consisted of a mixture of methanol and ultrapure water and 0.1% (v / v) acetic acid. Ions were generated using electrospray ionization (ESI) in the negative ion mode. Quantification was performed using a QTrap and / or triple quadrupole mass detector. The samples need to be diluted to fall within the linear range of the method, which is an analyte concentration of 1 ppb to 500 ppb in the injected sample. The LOQ is the limit of reliable detection, and a concentration of the compound detected in the sample below 1 ppb is not considered reliable and is therefore set to <LOQ.

[0114] Example 6

[0115] To further compare the exemplary enzymes according to the present invention with each other, a sequence alignment was performed. For this purpose, the Clustal Omega alignment tool of EMBL-EBI with default settings was used (https: / / www.ebi.ac.uk / Tools / msa / clustalo / ; Sievers et al. 2011. Mol. Syst. Biol. 7:539).

[0116] Table 5 below shows the identity of the amino acid sequences (Table 4) of 54 exemplary enzymes suitable for modifying trichothecenes.

[0117] Table 4: Amino acid sequences.

[0118]

[0119]

[0120]

[0121]

[0122]

[0123]

[0124] Table 5: Sequence identity of the amino acid sequences of SEQ ID NO.1 to SEQ ID NO.54 with each other.

[0125]

[0126]

[0127]

[0128]

[0129]

[0130]

[0131]

[0132]

[0133]

[0134]

[0135]

[0136]

Claims

1. A method for modifying at least one trichothecene, such as detoxifying at least one trichothecene, the method comprising forming a reaction mixture by contacting at least one polypeptide with a nutritional composition comprising at least one trichothecene, and with NAD(H) and / or NADP(H), preferably with NAD(H), the at least one polypeptide comprising an amino acid sequence having at least 70% sequence identity with an amino acid sequence selected from SEQ ID NO.1 to SEQ ID NO.54, preferably selected from SEQ ID NO.1 and SEQ ID NO.

34.

2. The method according to claim 1, wherein the method further comprises incubating the reaction mixture at a temperature of 5-40 °C, preferably at a temperature of 20-40 °C.

3. The method according to any one of the preceding claims, wherein the step of incubating the reaction mixture is carried out for at least 5 minutes, preferably at least 10 minutes.

4. The method according to any one of the preceding claims, wherein the at least one trichothecene is deoxynivalenol, and wherein the at least one polypeptide has at least 70% sequence identity with an amino acid sequence selected from SEQ ID NO.1 to SEQ ID NO.33, preferably with the amino acid sequence of SEQ ID NO:

1.

5. The method according to any one of claims 1-3, wherein the at least one trichothecene is 3-keto-deoxynivalenol, and wherein the at least one polypeptide comprises an amino acid sequence having at least 70% sequence identity with an amino acid sequence selected from SEQ ID NO.34 to SEQ ID NO.54, preferably with the amino acid sequence of SEQ ID NO.

34.

6. The method according to any one of claims 1-3, wherein the first trichothecene is deoxynivalenol, and wherein the first reaction mixture is formed by contacting a nutritional composition comprising deoxynivalenol with at least one polypeptide, the at least one polypeptide comprising an amino acid sequence having at least 70% sequence identity with an amino acid sequence selected from SEQ ID NO.1 to SEQ ID NO.33, preferably with the amino acid sequence of SEQ ID NO.1; wherein the second trichothecene is 3-keto-deoxynivalenol, and wherein the second reaction mixture is formed by contacting a nutritional composition comprising 3-keto-deoxynivalenol with at least one polypeptide, the at least one polypeptide comprising an amino acid sequence having at least 70% sequence identity with an amino acid sequence selected from SEQ ID NO.34 to SEQ ID NO.54, preferably with the amino acid sequence of SEQ ID NO.

34.

7. A composition, which is an additive for food and / or feed, for example; a feed or food, the composition being used to modify at least one trichothecene, for example to detoxify at least one trichothecene, the composition comprising at least one polypeptide, the at least one polypeptide comprising an amino acid sequence having at least 70% sequence identity with an amino acid sequence selected from SEQ ID NO.1 to SEQ ID NO.54, preferably selected from SEQ ID NO.1 and SEQ ID NO.

34.

8. The composition according to claim 7, wherein the composition comprises a cofactor selected from NAD(H) and NADP(H), preferably NAD(H).

9. The composition according to claim 7 or 8, wherein the composition comprises at least a first polypeptide and at least a second polypeptide, the at least first polypeptide comprising an amino acid sequence having at least 70% sequence identity with at least one amino acid sequence selected from SEQ ID NO.1 to SEQ ID NO.33, preferably with the amino acid sequence of SEQ ID NO.1; the at least second polypeptide comprising an amino acid sequence having at least 70% sequence identity with at least one amino acid sequence selected from SEQ ID NO.34 to SEQ ID NO.54, preferably with the amino acid sequence of SEQ ID NO.

34.

10. The composition according to any one of claims 7-9, wherein the composition comprises at least one carrier, the at least one carrier preferably being selected from bentonite, silica and carbohydrates.

11. Use of at least one polypeptide for modifying trichothecene, wherein the at least one polypeptide comprises an amino acid sequence having at least 70% sequence identity with an amino acid sequence selected from SEQ ID NO.1 to SEQ ID NO.54, preferably selected from SEQ ID NO.1 and SEQ ID NO.

34.

12. Use of at least one polypeptide for manufacturing an additive for feed and / or food, a feed and / or food composition, or a pharmaceutical composition, wherein the at least one polypeptide comprises an amino acid sequence having at least 70% sequence identity with an amino acid sequence selected from SEQ ID NO.1 to SEQ ID NO.54, preferably selected from SEQ ID NO.1 and SEQ ID NO.

34.

13. Use of at least one polypeptide for manufacturing biogas; bioethanol, DDGS; sugar, preferably sugar from sugarcane or sugar beet; corn oil, corn germ, corn germ meal, corn fiber, corn gluten, starch, especially corn starch; and / or silage; wherein the at least one polypeptide comprises an amino acid sequence having at least 70% sequence identity with an amino acid sequence selected from SEQ ID NO.1 to SEQ ID NO.54, preferably selected from SEQ ID NO.1 and SEQ ID NO.

34.

14. A host cell comprising at least one polypeptide and / or at least one polynucleotide encoding said at least one polypeptide, wherein said at least one polypeptide comprises an amino acid sequence having at least 70% sequence identity with an amino acid sequence selected from SEQ ID NO.1 to SEQ ID NO.54, preferably selected from SEQ ID NO.1 and SEQ ID NO.

34.

15. A polypeptide for modifying at least one trichothene, such as detoxifying at least one trichothene, said polypeptide comprising an amino acid sequence having at least 96.0% sequence identity with an amino acid sequence selected from SEQ ID NO.1 to SEQ ID NO.22, preferably with SEQ ID NO.1; or an amino acid sequence having at least 91.0% sequence identity with an amino acid sequence selected from SEQ ID NO.34 to SEQ ID NO.54, preferably with SEQ ID NO.

34.

16. A polypeptide for treating, ameliorating and / or preventing symptoms caused by mycotoxin poisoning, especially trichothene mycotoxin poisoning, wherein said polypeptide comprises an amino acid sequence having at least 70% sequence identity with an amino acid sequence selected from SEQ ID NO.1 to SEQ ID NO.54, preferably selected from SEQ ID NO.1 and SEQ ID NO.34.

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