Crop disease-resistant growth-promoting composition based on synergistic interaction of plant immune protein and thidiazuron

Abstract

The invention belongs to the technical field of agricultural biology, and particularly relates to a crop disease-resistant growth-promoting composition based on synergistic interaction of plant immune protein and thidiazuron, which comprises the following components: the plant immune protein which is selected from chitinase, beta1, 3 glucanase or PR protein and has the concentration of 0.1-10 mg / L; the concentration of the thidiazuron is 0.01 to 1 mg / L; according to the present invention, the optimal ratio of the immune protein and the thidiazuron is adopted, such that the limitation of the single component is avoided, and the synergistic effect of the immune protein and the thidiazuron can significantly improve the biomass and the disease resistance of the crops; the composition provided by the invention has the advantages of low cost, environmental protection, environmental friendliness, greenness and safety, and is especially suitable for the stable yield demand of high value-added crops under the stress condition, the effect of the composition is obviously better than that of a single component, and the environmental residue risk, the dependence on chemical pesticides and the use of the chemical pesticides can be reduced by utilizing the bioactive components.

Description

Technical Field

[0001] The present invention belongs to the field of agricultural biotechnology, and particularly relates to a crop disease-resistant and growth-promoting composition based on the synergistic effect of plant immune protein and thidiazuron. Background Art

[0002] At present, with the rapid development of science and technology, in the agricultural field, scientific and technological support is also indispensable. Crop diseases and growth regulation are key issues in agricultural production. Traditional methods mostly rely on the use of chemical pesticides and plant growth regulators to achieve crop disease resistance and growth promotion. However, the traditional methods have the following limitations: Chemical pesticides: Long-term use of chemical pesticides is likely to cause pathogens to develop drug resistance and will further harm the environment and human health.

[0003] Single plant growth regulator (such as thidiazuron): It can be used to promote cell division and crop growth. Although it can promote crop growth, it lacks disease resistance function, has no disease resistance effect, and excessive use may cause crop deformity or phytotoxicity.

[0004] Biological agents (such as plant immune protein): They can activate the plant immune system. Although they can induce crop disease resistance, when used alone, the growth promotion effect is not obvious, and the growth promotion effect is limited.

[0005] Compound of chemical pesticide and growth regulator: Such as fungicide + auxin, although it has the functions of disease resistance and growth promotion, the synergistic effect between the two is poor, and the problem of chemical residue is relatively prominent.

[0006] In summary, the disadvantages of the existing technology are reflected in the following aspects: 1. Single function: The existing technology cannot achieve the dual effects of disease resistance and growth promotion at the same time.

[0007] 2. Poor synergism: When chemical pesticides and growth regulators are compounded, phytotoxicity is easily caused or the drug efficacy is reduced.

[0008] 3. Environmental risk: The problem of chemical pesticide residue is serious, which does not conform to the development trend of green agriculture.

[0009] Based on the existing technology situation, the present invention newly proposes a composition based on the synergistic effect of plant immune protein and thidiazuron. Summary of the Invention

[0010] To solve the above problems existing in the prior art, the present invention provides a crop disease-resistant and growth-promoting composition based on the synergistic effect of plant immune protein and thidiazuron. It adopts an optimized ratio of immune protein + thidiazuron to avoid the limitations of single components. The synergistic effect of the two significantly improves the crop biomass and disease resistance; enhances crop health and yield. The effect of the composition is significantly better than that of single components. Moreover, by using bioactive ingredients, it can reduce the environmental residue risk, reduce the dependence on chemical pesticides, reduce the use of chemical pesticides, is environmentally friendly, green and safe, and is particularly suitable for the stable yield demand of high-value crops under adversity conditions.

[0011] To achieve the above object, the present invention provides the following technical solution: A crop disease-resistant and growth-promoting combination based on the synergistic effect of plant immune protein and thidiazuron, comprising: Plant immune protein: selected from chitinase, β1,3-glucanase or PR protein (such as PR1, PR2), with a concentration of 0.1~10 mg / L; Thidiazuron: with a concentration of 0.01~1 mg / L; Auxiliary components: surfactants (such as Tween 80), stabilizers (such as glycerol) or trace elements can be added.

[0012] As a preferred technical solution of the crop disease-resistant and growth-promoting composition based on the synergistic effect of plant immune protein and thidiazuron of the present invention, the preparation method of the composition comprises the following steps: Step 1: Dissolve the plant immune protein in a buffer solution (such as PBS, pH 7.0); Step 2: Add the thidiazuron solution and stir to mix evenly; Step 3: Adjust the pH to 6.0~7.5, filter and sterilize, and then subpackage.

[0013] As a preferred technical solution of the crop disease-resistant and growth-promoting composition based on the synergistic effect of plant immune protein and thidiazuron of the present invention, the application method of the composition includes: Foliar spraying: Dilute 100~500 times and apply during the crop growth period or at the initial stage of disease; Seed treatment: Soak the seeds for 1~2 hours, dry them and then sow.

[0014] As a preferred technical solution of the crop disease-resistant and growth-promoting composition based on the synergistic effect of plant immune protein and thidiazuron of the present invention, chitosan can be used to replace chitinase.

[0015] As a preferred technical solution of the crop disease-resistant and growth-promoting composition based on the synergistic effect of plant immune protein and thidiazuron of the present invention, the thidiazuron derivative can be replaced with forchlorfenuron.

[0016] Compared with the prior art, the beneficial effects of the present invention are: 1. The present invention has dual effects. By activating the systemic resistance of plants through immune proteins, it activates the resistance of crops, enhances the defense ability against pathogenic bacteria, and can achieve disease resistance. At the same time, through thidiazuron, it promotes cell division and differentiation, alleviates the growth inhibition caused by immune response, and can achieve growth promotion.

[0017] 2. The present invention adopts an optimized ratio of immune protein + thidiazuron to avoid the limitations of single components. The synergistic effect of the two significantly improves the biomass and disease resistance of crops, enhances the health and yield of crops, and the effect of the composition is significantly better than that of single components.

[0018] 3. The present invention is environmentally friendly, green and safe. By using bioactive ingredients, it reduces the risk of environmental residues, reduces the dependence on chemical pesticides, and reduces the use of chemical pesticides.

[0019] 4. The preparation of the present invention is simple and convenient.

[0020] 5. The present invention can be used for foliar fertilization or seed treatment, with flexible application methods and strong adaptability. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] The drawings are used to provide a further understanding of the present invention, and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention, and do not constitute a limitation to the present invention. In the drawings: Figure 1 is the principle block diagram of the present invention; Figure 2 is the control diagram of the tomato disease resistance and growth promotion test of the present invention. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0022] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention. Embodiment

[0023] Please refer to Figure 1-2 , the present invention provides the following technical solutions: A crop disease resistance and growth promotion composition based on the synergistic effect of plant immune protein and thidiazuron, comprising: Plant immune protein: selected from chitinase, β1,3-glucanase or PR protein (such as PR1, PR2), with a concentration of 0.1 - 10 mg / L; Thidiazuron: with a concentration of 0.01 - 1 mg / L; Auxiliary components: surfactants (such as Tween 80), stabilizers (such as glycerol) or trace elements can be added.

[0024] Other alternative solutions: Replace the type of immune protein: For example, use chitosan to replace chitinase, but the effect is slightly inferior; Adjust thidiazuron derivatives: For example, switch to forchlorfenuron (CPPU), but the cost is relatively high.

[0025] The preparation method of the composition includes the following steps: Step 1: Dissolve the plant immune protein in a buffer solution (such as PBS, pH 7.0); Step 2: Add the thidiazuron solution and stir to mix evenly; Step 3: Adjust the pH to 6.0 - 7.5, filter and sterilize, and then subpackage.

[0026] The application methods of the composition include: Foliar spraying: Dilute 100 - 500 times and apply during the growth period of the crop or at the initial stage of the disease; Seed treatment: Soak the seeds for 1 - 2 hours, dry them and then sow.

[0027] Refer to Figure 1 As shown, where: The core functions of thidiazuron (plant growth regulator): Cell division: By activating cytokinin synthesis genes (such as _CYCD3_), it can promote cell division and expansion; Hormone balance: By reducing the content of abscisic acid (ABA), it can relieve stress inhibition and enhance the growth potential of crops.

[0028] The core functions of plant immune protein (biostimulant): Immune activation: Induce the synthesis of pathogenesis-related proteins (PR proteins), and enhance disease resistance through salicylic acid (SA) and jasmonic acid (JA) signaling pathways; Stress resistance protection: It can enhance the activities of superoxide dismutase (SOD) and peroxidase (POD) to scavenge free radicals.

[0029] The two have the following synergistic effects: Functional complementarity: Promote rapid growth through thidiazuron and provide disease protection through immune protein, which can avoid the problem of "easy to grow tall and susceptible to diseases".

[0030] Metabolic synergism: The small molecule peptides in the immune protein can carry thidiazuron through the plant epidermis, and the utilization rate can be increased by 20% - 30%.

[0031] Application examples of the composition: Yield increase of fruits and vegetables: When used during the flowering period of strawberries, the single fruit weight can increase by 15%, and the incidence of gray mold can be reduced by 40%; Field stress resistance: Sprayed during the jointing stage of wheat, the lodging rate can be reduced by 50%, and the control effect against Fusarium head blight can be increased by 35%.

[0032] This composition is particularly suitable for high-value crops (such as fruits and vegetables) and the stable yield requirements under adverse conditions.

[0033] Refer to Figure 2 As shown, in the tomato disease resistance and growth promotion experiment: Experimental treatment group: Spray with immune protein (1.2 mg / L) + thidiazuron (0.5 mg / L).

[0034] Experimental control group: Single immune protein or thidiazuron.

[0035] Final experimental results: The disease incidence rate of the treatment group can be reduced by 40%, and the yield can be increased by 25%, which is significantly better than the control group (P < 0.05).

[0036] Other instructions: Thidiazuron: It is a urea-type plant growth regulator. At low concentrations, it has extremely strong cell division activity. It can induce plant cell division, and the ability to form callus is more than a thousand times higher than that of general cytokinins. It can also delay plant senescence, enhance its stress resistance, promote plant photosynthesis, improve crop yield and quality. Other names: Defoliant, Thidiazuron, Dropp, etc. Chemical name: 1-phenyl-3-(1,2,3-thiadiazol-5-yl)urea.

[0037] Plant immune protein: It is a new type of biological pesticide, also known as protein pesticide, activating protein, immune protein, plant vaccine. It is non-toxic and residue-free, and is friendly to the environment. It is a heat-stable protein extracted from fungi or bacteria. It is composed of proteins with molecular weights ranging from 35 to 68 KD. Through its interaction with plant surface receptor proteins, it can induce the signal transduction of plants, activate a series of metabolic regulation reactions of plants, so that plants can develop resistance to pests and diseases, promote plant growth, improve crop quality, and increase crop yield. Regarding the mechanism of action of this type of protein pesticide, generally speaking, after this type of protein is applied to plants, it first binds to the receptor proteins on the plant surface. After the receptor proteins of the plants receive the signal transduction of the activating protein, a series of metabolic reactions in the plants are activated, promoting the synthesis of salicylic acid and jasmonic acid in the plants, and then synthesizing phytoalexins and other disease-resistant related proteins through the salicylic acid or jasmonic acid pathway to achieve the effect of disease and pest control. After the protein pesticide treats plants, the contents of proline, peroxidase, root dehydrogenase, etc. in the plants all increase. These enzymes are directly related to plant stress resistance. Activating protein can activate and induce these enzymes to enhance their activity, which means enhancing the disease and pest resistance and stress resistance of plants.

[0038] In addition, the content not described in detail in this embodiment belongs to the category of prior art and common general knowledge.

[0039] In this implementation scheme: The present invention has dual effects. By activating the plant systemic resistance through immune proteins, activating the crop resistance, enhancing the defense ability against pathogenic bacteria, it can achieve disease resistance (such as fungal and bacterial diseases). At the same time, through thidiazuron, it promotes cell division and differentiation, alleviates the growth inhibition caused by immune response, and can achieve growth promotion (increasing production by 10% - 30%).

[0040] The present invention adopts an optimized ratio of immune protein + thidiazuron, avoiding the limitations of a single component. The synergistic effect of the two significantly improves the crop biomass and disease resistance, enhances the crop health and yield, and the effect of the composition is significantly better than that of a single component.

[0041] The present invention is environmentally friendly, green and safe. By using bioactive ingredients, it reduces the environmental residue risk, reduces the dependence on chemical pesticides, and reduces the use of chemical pesticides (by more than 50%).

[0042] The preparation of the present invention is simple and convenient.

[0043] The present invention can be used when foliar fertilization or seed treatment is selected, with a flexible application method and strong adaptability.

[0044] Finally, it should be noted that the above are only the preferred embodiments of the present invention and are not used to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements for some of the technical features. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A crop disease-resistant and growth-promoting combination based on the synergistic effect of plant immune protein and thidiazuron, characterized by including: Plant immune protein: selected from chitinase, β1,3-glucanase or PR protein (such as PR1, PR2), with a concentration of 0.1 - 10 mg / L; Thidiazuron: with a concentration of 0.01 - 1 mg / L; Auxiliary components: surfactants (such as Tween 80), stabilizers (such as glycerol) or trace elements can be added.

2. The crop disease-resistant and growth-promoting composition based on the synergistic effect of plant immune protein and thidiazuron according to claim 1, wherein: The preparation method of this composition includes the following steps: Step 1: Dissolve the plant immune protein in a buffer solution (such as PBS, pH 7.0); Step 2: Add the thidiazuron solution and stir to mix evenly; Step 3: Adjust the pH to 6.0 - 7.5, filter and sterilize, and then subpackage.

3. A crop disease-resistant and growth-promoting composition based on the synergistic effect of plant immune protein and thidiazuron according to claim 1, characterized in that: The application method of this composition includes: Foliar spraying: After diluting 100 - 500 times, apply during the growth period of the crop or at the initial stage of the disease; Seed treatment: Soak the seeds for 1 - 2 hours, dry them and then sow.

4. A crop disease-resistant and growth-promoting composition based on the synergistic effect of plant immune protein and thidiazuron according to claim 1, characterized in that: Chitosan can be used to replace chitinase.

5. A crop disease-resistant and growth-promoting composition based on the synergistic effect of plant immune protein and thidiazuron according to claim 1, characterized in that: The thidiazuron derivative can be changed to forchlorfenuron.

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