Biological prevention and control fungicide for plant diseases and insect pests of hemp plants and preparation method of biological prevention and control fungicide

Abstract

The invention relates to the technical field of hemp plant disease and insect pest control, in particular to a hemp plant disease and insect pest biological control fungicide and a preparation method thereof. The invention provides a biological control fungicide for plant diseases and insect pests of cannabis sativa plants. The microbial agent is prepared from the following components in parts by mass: 10 to 15 parts of bifidobacterium bifidum, 12 to 16 parts of paenibacillus polymyxa, 16 to 20 parts of bacillus amyloliquefaciens, 12 to 16 parts of burkholderia, 13 to 17 parts of pythium oligandrum, 6 to 10 parts of lactobacillus plantarum, 15 to 19 parts of bacillus velezensis, 15 to 19 parts of pseudomonas aeruginosa and 12 to 16 parts of metarhizium anisopliae. The biological control fungicide for the plant diseases and insect pests of the cannabis sativa has a good control effect on various plant diseases and insect pests of the cannabis sativa, has a remarkable growth promoting effect on the cannabis sativa when being used as a cannabis sativa seed dressing agent, and has a good application prospect.

Description

Technical Field

[0001] The present invention relates to the technical field of cannabis pest and disease control, and particularly relates to a biological control bactericide for cannabis plant pests and diseases and a preparation method thereof. Background Art

[0002] As an important cash crop, cannabis faces threats from various pests and diseases throughout its growth cycle. These biotic stress factors seriously affect the yield and quality of cannabis. During the cultivation of cannabis, fungal diseases are the most common and severely harmful type of diseases. Root rot is mainly caused by Fusarium spp. and Rhizoctonia solani, manifested as root rot, plant wilting and even death. In severe cases, it can lead to the complete failure of the entire hemp field. Leaf spot disease is mostly caused by pathogenic bacteria such as Cercospora cannabis and Alternaria alternata, forming irregular yellowish-brown to brown spots on the leaves, with darker edges. In the later stage, small black dots are scattered on the disease spots, seriously affecting the photosynthesis of the leaves. Anthracnose is caused by Colletotrichum spp., which can infect the stems, leaves and fruits of cannabis, forming sunken black spots, resulting in hindered plant growth.

[0003] In addition to fungal diseases, bacterial diseases such as angular leaf spot also pose a serious threat to cannabis. This disease mainly infects the leaves, initially forming water-soaked small spots, which then turn into brown polygonal spots. In severe cases, the leaves are densely covered with disease spots, resulting in local or complete drying and early shedding of the leaves, seriously affecting the normal growth of the plant. In addition, root-knot nematode disease caused by nematodes is particularly prominent in continuous cannabis cropping fields. Nematodes infect the roots to form root knots, hindering the absorption of water and nutrients, resulting in poor plant growth and reduced yield.

[0004] In terms of pests, the hemp borer is one of the most serious pests of cannabis. The larvae of the hemp borer bore into the stems, causing the stems to be hollow and easily breakable. At the same time, they spread pathogenic bacteria, triggering secondary diseases. Research shows that the damage of the hemp borer can cause a yield loss of industrial hemp as high as 30% - 50%, seriously threatening the economic benefits of cannabis cultivation. In addition, the jute cutworm is also a common pest of cannabis. Its larvae feed on the leaves. In severe cases, the leaves can be eaten up, leaving only the leaf veins, seriously affecting the photosynthesis and normal growth of the plant.

[0005] The occurrence and prevalence of these diseases and pests are affected by many factors. Continuous cultivation leads to the accumulation of pathogens and insect eggs in the soil, aggravating the occurrence of diseases and pests. Climate conditions such as high temperature and high humidity are conducive to the occurrence and spread of most fungal diseases and some insect pests. Improper cultivation management, such as excessive density, poor ventilation, excessive nitrogen fertilizer, etc., will also reduce plant resistance and aggravate the damage of diseases and pests. It is worth noting that with the expansion of industrial hemp planting areas and the increase in continuous cropping years, the types of diseases and pests are becoming more complex, the degree of harm is increasing, and the need for effective prevention and control measures is becoming more urgent.

[0006] For a long time, chemical pesticides have dominated the control of cannabis pests and diseases. Their quick effect and ease of use have made them popular among growers. However, with the increasing emphasis on food safety, environmental protection and sustainable agriculture, the application of chemical pesticides in the control of cannabis pests and diseases is facing increasingly severe challenges and limitations. In this context, biological control agents have become an important research direction for green control of cannabis pests and diseases due to their advantages such as environmental friendliness, precise targets, and low resistance. Therefore, how to provide a biological control agent for cannabis plant pests and diseases is a problem that technicians in this field urgently need to solve. Summary of the invention

[0007] The purpose of the present invention is to provide a biological control agent for hemp plant diseases and insect pests and a preparation method thereof.

[0008] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0009] The invention provides a biological control fungicide for hemp plant diseases and insect pests, which is prepared from the following components in parts by mass: 10-15 parts of Bifidobacterium bifidum, 12-16 parts of Paenibacillus polymyxa, 16-20 parts of Bacillus amyloliquefaciens, 12-16 parts of Burkholderia, 13-17 parts of Pythium oligandrum, 6-10 parts of Lactobacillus plantarum, 15-19 parts of Bacillus velezensis, 15-19 parts of Pseudomonas aeruginosa, and 12-16 parts of Metarhizium anisopliae.

[0010] Preferably, the bacterial content of Bifidobacterium bifidum, Paenibacillus polymyxa, Bacillus amyloliquefaciens, Burkholderia, Pythium oligandrum, Lactobacillus plantarum, Bacillus velezensis, Pseudomonas aeruginosa and Metarhizium anisopliae is 400 million to 700 million per gram.

[0011] Preferably, it also includes 2 to 4 parts of biostimulants; the biostimulants are: konjac glucomannan, coriolus versicolor polysaccharide and tuckahoe tea polysaccharide; the mass ratio of konjac glucomannan, coriolus versicolor polysaccharide and tuckahoe tea polysaccharide is 3 to 5:12 to 16:15 to 25.

[0012] The present invention also provides a method for preparing the biological control bacterial agent for pests and diseases of cannabis plants, which comprises mixing Bifidobacterium bifidum, Paenibacillus polymyxa, Bacillus amyloliquefaciens, Burkholderia sp., Pythium oligandrum, Lactobacillus plantarum, Bacillus velezensis, Pseudomonas aeruginosa, Metarhizium pingshaense and a biostimulant.

[0013] The present invention also provides the use of the biological control bacterial agent for pests and diseases of cannabis plants or the biological control bacterial agent for pests and diseases of cannabis plants prepared by the preparation method in the preparation of a cannabis seed dressing agent.

[0014] The present invention also provides a cannabis seed dressing agent, which comprises 5-15 parts of the biological control bacterial agent for pests and diseases of cannabis plants or the biological control bacterial agent for pests and diseases of cannabis plants prepared by the preparation method, 2-5 parts of a wetting agent, 2-5 parts of a film-forming agent and 2-5 parts of a thickening agent.

[0015] Preferably, the wetting agent is sodium dodecyl sulfate, calcium dodecylbenzenesulfonate or sodium methylene bisnaphthalenesulfonate; the film-forming agent is sodium carboxymethyl cellulose, polyvinyl alcohol or sodium alginate; and the thickening agent is xanthan gum, magnesium aluminum silicate or polyvinyl alcohol.

[0016] The present invention also provides a method for preparing the cannabis seed dressing agent, which comprises mixing the biological control bacterial agent for pests and diseases of cannabis plants, the wetting agent, the film-forming agent and the thickening agent.

[0017] The present invention also provides a method for using the cannabis seed dressing agent or the cannabis seed dressing agent prepared by the preparation method, which comprises mixing the cannabis seed dressing agent with water and then adding cannabis seeds.

[0018] Preferably, the mass ratio of the cannabis seed dressing agent, water and cannabis seeds is 1:8-20:800-900.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] The invention provides a biological control agent for hemp plant diseases and insect pests, wherein the use of Bifidobacterium bifidum can control pseudoperonospora of hemp and prevent hemp downy mildew; Paenibacillus polymyxa can control cercospora of hemp and prevent fungal diseases of cercospora olive of hemp, such as brown spot disease, gray mold disease, etc. Bacillus amyloliquefaciens can control fungal diseases of Alternaria genus and Fusarium oxysporum, and prevent black spot disease of hemp caused by Fusarium genus. Burkholderia and Pythium oligandrum can control Fusarium series diseases of hemp, such as Fusarium stem canker, Fusarium basal rot and root rot, etc. Lactobacillus plantarum can control Botrytis cinerea, gray mold disease of hemp, borers, etc. Pseudomonas aeruginosa and Metarhizium flatsandii can control hemp winter borer, small cutworms and yellow-winged termites, etc. Paenibacillus polymyxa and Bacillus velez can control a variety of pests, such as flea beetles, borers, etc. Therefore, the biological control agent for hemp plant diseases and insect pests provided by the present invention has good control effects on various diseases and insect pests of hemp, and when used as a seed dressing agent for hemp seeds, it also has a significant growth-promoting effect on hemp, and has good application prospects. DETAILED DESCRIPTION

[0021] The invention provides a biological control fungicide for hemp plant diseases and insect pests, which is prepared from the following components in parts by weight: 10-15 parts of Bifidobacterium bifidum, 12-16 parts of Paenibacillus polymyxa, 16-20 parts of Bacillus amyloliquefaciens, 12-16 parts of Burkholderia, 13-17 parts of Pythium oligandrum, 6-10 parts of Lactobacillus plantarum, 15-19 parts of Bacillus velez, 15-19 parts of Pseudomonas aeruginosa, and 12-16 parts of Metarhizium anisopliae; preferably 11-14 parts of Bifidobacterium bifidum, 13-15 parts of Paenibacillus polymyxa, 17-19 parts of Bacillus amyloliquefaciens, 13-15 parts of Burkholderia, 14-16 parts of Pythium oligandrum, and 7-9 parts of Lactobacillus plantarum. , 16-18 parts of Bacillus Velez, 16-18 parts of Pseudomonas aeruginosa, and 13-15 parts of Metarhizium anisopliae; further preferably 12-13 parts of Bifidobacterium bifidum, 14 parts of Paenibacillus polymyxa, 18 parts of Bacillus amyloliquefaciens, 14 parts of Burkholderia, 15 parts of Pythium oligandrum, 8 parts of Lactobacillus plantarum, 17 parts of Bacillus Velez, 17 parts of Pseudomonas aeruginosa, and 17 parts of Metarhizium anisopliae; more preferably 13 parts of Bifidobacterium bifidum, 14 parts of Paenibacillus polymyxa, 18 parts of Bacillus amyloliquefaciens, 14 parts of Burkholderia, 15 parts of Pythium oligandrum, 8 parts of Lactobacillus plantarum, 17 parts of Bacillus Velez, 17 parts of Pseudomonas aeruginosa, and 17 parts of Metarhizium anisopliae.

[0022] In the present invention, the bacterial content of Bifidobacterium bifidum, Paenibacillus polymyxa, Bacillus amyloliquefaciens, Burkholderia, Pythium oligandrum, Lactobacillus plantarum, Bacillus velezensis, Pseudomonas aeruginosa and Metarhizium anisopliae is 400 million to 700 million / g; preferably 500 million to 600 million / g; more preferably 600 million / g.

[0023] In the present invention, 2 to 4 parts of biostimulant are also included, preferably 3 parts.

[0024] In the present invention, the biostimulant is: konjac glucomannan, coriolus versicolor polysaccharide and tuckahoe tea polysaccharide; the mass ratio of konjac glucomannan, coriolus versicolor polysaccharide and tuckahoe tea polysaccharide is 3-5:12-16:15-25; preferably 4:13-15:17-23; further preferably 4:14:19-21; more preferably 4:14:20.

[0025] The present invention also provides a method for preparing the biological control agent for hemp plant diseases and insect pests, which comprises mixing Bifidobacterium bifidum, Paenibacillus polymyxa, Bacillus amyloliquefaciens, Burkholderia, Pythium oligandrum, Lactobacillus plantarum, Bacillus Velezii, Pseudomonas aeruginosa, Metarhizium anisopliae and a biological stimulant.

[0026] The present invention also provides the use of the biological control fungus agent for hemp plant diseases and insect pests or the biological control fungus agent for hemp plant diseases and insect pests prepared by the preparation method in the preparation of hemp seed dressing agents.

[0027] The present invention also provides a hemp seed dressing agent, comprising 5-15 parts of the biological control fungus agent for hemp plant diseases and insect pests or the biological control fungus agent for hemp plant diseases and insect pests obtained by the preparation method, 2-5 parts of a wetting agent, 2-5 parts of a film-forming agent, and 2-5 parts of a thickener; preferably, 7-13 parts of the biological control fungus agent for hemp plant diseases and insect pests or the biological control fungus agent for hemp plant diseases and insect pests obtained by the preparation method, 3-4 parts of a wetting agent, 3-4 parts of a film-forming agent, and 3-4 parts of a thickener; further preferably, 9-11 parts of the biological control fungus agent for hemp plant diseases and insect pests or the biological control fungus agent for hemp plant diseases and insect pests obtained by the preparation method, 4 parts of a wetting agent, 4 parts of a film-forming agent, and 4 parts of a thickener; more preferably, 10 parts of the biological control fungus agent for hemp plant diseases and insect pests or the biological control fungus agent for hemp plant diseases and insect pests obtained by the preparation method, 4 parts of a wetting agent, 4 parts of a film-forming agent, and 4 parts of a thickener.

[0028] In the present invention, the wetting agent is sodium dodecyl sulfate, calcium dodecylbenzene sulfonate or sodium methylene dinaphthalene sulfonate; preferably sodium dodecyl sulfate.

[0029] In the present invention, the film-forming agent is sodium carboxymethyl cellulose, polyvinyl alcohol or sodium alginate; preferably sodium carboxymethyl cellulose.

[0030] In the present invention, the thickener is xanthan gum, magnesium aluminum silicate or polyvinyl alcohol; preferably xanthan gum.

[0031] The present invention also provides a method for preparing the hemp seed dressing agent, which comprises mixing the hemp plant disease and insect pest biological control agent, a wetting agent, a film-forming agent and a thickener.

[0032] The present invention also provides a method for using the hemp seed dressing agent or the hemp seed dressing agent prepared by the preparation method, which comprises mixing the hemp seed dressing agent with water and then adding hemp seeds to mix.

[0033] In the present invention, the mass ratio of the hemp seed dressing agent, water and hemp seeds is 1:8-20:800-900; preferably 1:10-18:820-880; further preferably 1:12-16:840-860; more preferably 1:14:850.

[0034] Among the bacterial agents used in the present invention, Bifidobacterium bifidum (Beina Biological, BNCC364162), Bacillus polymyxa (Beina Biological, BNCC194976), Bacillus amyloliquefaciens (Beina Biological, BNCC336388), Burkholderia (CCTCC NO: M 2021309), Pythium oligandrum (preservation number is CGMCC NO.20224), Lactobacillus plantarum (Beina Biological, BNCC192575), Bacillus velez (preservation number is CGMCC NO.25882), Pseudomonas aeruginosa (Beina Biological, BNCC338118), Metarhizium anisopliae (Beina Biological, BNCC336563), Bacillus subtilis (Beina Biological, BNCC359371), and Bacillus megaterium (Beina Biological, BNCC336464).

[0035] The konjac glucomannan (product number: 10088693381340; JD store: Taigong Industrial Automation Store), versicolor polysaccharide (Xi'an Ruilin Biotechnology Co., Ltd.) and Fucha polysaccharide (preparation method reference "Wang Fan's Study on the Structural Characteristics, Immunomodulatory Activity and Mechanism of Action of Shaanxi Fucha Polysaccharide").

[0036] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0037] Example 1

[0038] A biological control agent for hemp plant diseases and insect pests is prepared by mixing 10 parts of Bifidobacterium bifidum, 12 parts of Paenibacillus polymyxa, 16 parts of Bacillus amyloliquefaciens, 12 parts of Burkholderia, 13 parts of Pythium oligandrum, 6 parts of Lactobacillus plantarum, 15 parts of Bacillus velezensis, 15 parts of Pseudomonas aeruginosa and 12 parts of Metarhizium anisopliae.

[0039] Among them, the bacterial content of Bifidobacterium bifidum, Bacillus polymyxa, Bacillus amyloliquefaciens, Burkholderia, Pythium oligandrum, Lactobacillus plantarum, Bacillus velezensis, Pseudomonas aeruginosa and Metarhizium anisopliae is 400 million / g.

[0040] Example 2

[0041] A biological control agent for diseases and insect pests of cannabis plants is prepared by mixing 15 parts of Bifidobacterium bifidum, 16 parts of Paenibacillus polymyxa, 20 parts of Bacillus amyloliquefaciens, 16 parts of Burkholderia, 17 parts of Pythium oligandrum, 10 parts of Lactobacillus plantarum, 19 parts of Bacillus velez, 19 parts of Pseudomonas aeruginosa and 16 parts of Metarhizium anisopliae.

[0042] Among them, the bacterial content of the Bifidobacterium bifidum, Bacillus polymyxa, Bacillus amyloliquefaciens, Burkholderia, Pythium oligandrum, Lactobacillus plantarum, Bacillus velezensis, Pseudomonas aeruginosa and Metarhizium anisopliae is 700 million / g.

[0043] Example 3

[0044] A biological control agent for hemp plant diseases and insect pests is prepared by mixing 13 parts of Bifidobacterium bifidum, 14 parts of Paenibacillus polymyxa, 18 parts of Bacillus amyloliquefaciens, 14 parts of Burkholderia, 15 parts of Pythium oligandrum, 8 parts of Lactobacillus plantarum, 17 parts of Bacillus velez, 17 parts of Pseudomonas aeruginosa and 17 parts of Metarhizium anisopliae.

[0045] Among them, the bacterial content of the Bifidobacterium bifidum, Bacillus polymyxa, Bacillus amyloliquefaciens, Burkholderia, Pythium oligandrum, Lactobacillus plantarum, Bacillus velezensis, Pseudomonas aeruginosa and Metarhizium anisopliae is 600 million / g.

[0046] Example 4

[0047] A biological control agent for hemp plant diseases and insect pests is prepared by mixing 10 parts of Bifidobacterium bifidum, 12 parts of Paenibacillus polymyxa, 16 parts of Bacillus amyloliquefaciens, 12 parts of Burkholderia, 13 parts of Pythium oligandrum, 6 parts of Lactobacillus plantarum, 15 parts of Bacillus velez, 15 parts of Pseudomonas aeruginosa, 12 parts of Metarhizium anisopliae, and 2 parts of a biostimulant;

[0048] The biostimulant is: konjac glucomannan, versicolor polysaccharide and tuckahoe tea polysaccharide, with a mass ratio of 3:12:15;

[0049] Among them, the bacterial content of Bifidobacterium bifidum, Bacillus polymyxa, Bacillus amyloliquefaciens, Burkholderia, Pythium oligandrum, Lactobacillus plantarum, Bacillus velezensis, Pseudomonas aeruginosa and Metarhizium anisopliae is 400 million / g.

[0050] Example 5

[0051] A biological control agent for hemp plant diseases and insect pests is prepared by mixing 15 parts of Bifidobacterium bifidum, 16 parts of Paenibacillus polymyxa, 20 parts of Bacillus amyloliquefaciens, 16 parts of Burkholderia, 17 parts of Pythium oligandrum, 10 parts of Lactobacillus plantarum, 19 parts of Bacillus velez, 19 parts of Pseudomonas aeruginosa, 16 parts of Metarhizium anisopliae, and 4 parts of a biostimulant;

[0052] The biostimulant is: konjac glucomannan, versicolor polysaccharide and tuckahoe tea polysaccharide, with a mass ratio of 5:16:25;

[0053] Among them, the bacterial content of the Bifidobacterium bifidum, Bacillus polymyxa, Bacillus amyloliquefaciens, Burkholderia, Pythium oligandrum, Lactobacillus plantarum, Bacillus velezensis, Pseudomonas aeruginosa and Metarhizium anisopliae is 700 million / g.

[0054] Example 6

[0055] A biological control agent for diseases and insect pests of cannabis plants is prepared by mixing 13 parts of Bifidobacterium bifidum, 14 parts of Paenibacillus polymyxa, 18 parts of Bacillus amyloliquefaciens, 14 parts of Burkholderia, 15 parts of Pythium oligandrum, 8 parts of Lactobacillus plantarum, 17 parts of Bacillus velezensis, 17 parts of Pseudomonas aeruginosa, 17 parts of Metarhizium anisopliae, and 3 parts of a biostimulant;

[0056] The biostimulant is: konjac glucomannan, versicolor polysaccharide and tuckahoe tea polysaccharide, with a mass ratio of 4:14:20;

[0057] Among them, the bacterial content of the Bifidobacterium bifidum, Bacillus polymyxa, Bacillus amyloliquefaciens, Burkholderia, Pythium oligandrum, Lactobacillus plantarum, Bacillus velezensis, Pseudomonas aeruginosa and Metarhizium anisopliae is 600 million / g.

[0058] Example 7

[0059] A hemp seed dressing agent is prepared by mixing 5 parts of the hemp plant disease and insect pest biological control agent prepared in Example 4, 2 parts of a wetting agent, 2 parts of a film-forming agent, and 2 parts of a thickener.

[0060] The wetting agent is sodium methylene dinaphthalene sulfonate; the film-forming agent is sodium alginate; and the thickener is polyvinyl alcohol.

[0061] Example 8

[0062] A hemp seed dressing agent is prepared by mixing 15 parts of the hemp plant disease and insect pest biological control agent prepared in Example 5, 5 parts of a wetting agent, 5 parts of a film-forming agent, and 5 parts of a thickener.

[0063] The wetting agent described above is calcium dodecylbenzenesulfonate; the film-forming agent is polyvinyl alcohol; the thickening agent is magnesium aluminum silicate.

[0064] Example 9

[0065] A cannabis seed dressing agent can be prepared by mixing 10 parts of the biological control agent for cannabis plant diseases and pests prepared in Example 6, 3 parts of a wetting agent, 3 parts of a film-forming agent, and 3 parts of a thickening agent.

[0066] The wetting agent described above is sodium dodecyl sulfate; the film-forming agent is sodium carboxymethyl cellulose; the thickening agent is xanthan gum.

[0067] Example 10

[0068] A method for using a cannabis seed dressing agent is to mix the cannabis seed dressing agent prepared in Example 7 with water and then add cannabis seeds; the mass ratio of the cannabis seed dressing agent, water, and cannabis seeds is 1:20:900.

[0069] Example 11

[0070] A method for using a cannabis seed dressing agent is to mix the cannabis seed dressing agent prepared in Example 8 with water and then add cannabis seeds; the mass ratio of the cannabis seed dressing agent, water, and cannabis seeds is 1:8:800.

[0071] Example 12

[0072] A method for using a cannabis seed dressing agent is to mix the cannabis seed dressing agent prepared in Example 9 with water and then add cannabis seeds; the mass ratio of the cannabis seed dressing agent, water, and cannabis seeds is 1:14:850.

[0073] Comparative Example 1

[0074] Other methods are the same as those in Example 12, except that Bacillus velezensis and Pseudomonas aeruginosa in Example 6 are replaced with an equal amount of Bacillus subtilis and Bacillus megaterium.

[0075] Comparative Example 2

[0076] Other methods are the same as those in Example 12, except that Burkholderia and Pythium oligandrum in Example 6 are both replaced with an equal amount of Bacillus velezensis.

[0077] Comparative Example 3

[0078] Other methods are the same as those in Example 12, except that the biostimulant in Example 6 is replaced with an equal amount of distilled water.

[0079] Comparative Example 4

[0080] Other methods are the same as those in Example 12, except that both konjac glucomannan and polysaccharide from Coriolus versicolor in Example 6 are replaced with an equal amount of polysaccharide from Fuzhuan tea.

[0081] Example 13

[0082] Verification test on the prevention and control of hemp diseases and pests. The test site was the hemp planting experimental field of the Heilongjiang Academy of Agricultural Sciences, and the tested variety was Longdamalhao. After dividing the hemp planting experimental field into planting plots, three of the plots were randomly treated in each test group.

[0083] Among them, the length of each plot was 20.0 m, the row spacing was 0.15 m, there were 30 rows in the plot, and the plot area was 90 m 2 , flat sowing and close planting, manual drilling, and the number of sown seeds was 500 seeds / m 2 ; Sowing was carried out in late April, and harvested at the appropriate time during the technological maturity period. Five points were fixed in each plot, and 2 m was taken at each point 2 for field investigation and yield measurement.

[0084] Among them, the preparation of a hemp seed dressing agent in Example 9 was used as the experimental group, and it was used with reference to the method in Example 12.

[0085] Taking Comparative Example 1 to Comparative Example 4 as Control Group 1 to Control Group 4. Using no treatment on hemp seeds as the blank control group.

[0086] Control effect of diseases and pests (%) = (data in the blank control area - data in the treated area) / data in the blank control area × 100%

[0087] Table 1 Control effect of diseases and pests

[0088]

[0089]

[0090] It can be seen from the content recorded in Table 1 that the experimental group has good control effects on various hemp diseases and pests, and its control effect is significantly better than that of other control groups.

[0091] Table 2 Growth promotion effect on hemp

[0092] Group Emergence rate % Original stem yield kg / mu Long fiber rate % Fiber yield kg / mu Experimental group 62.3 1183.3 16.4 126.7 Control group 1 54.5 1102.9 15.6 116.6 Control group 2 58.0 1134.7 15.9 117.1 Control group 3 44.7 983.7 13.8 106.2 Control group 4 47.3 1028.4 13.9 109.3

[0093] It can be seen from the records in Table 2 that the bacterial agent combination provided by the present invention also has a significant promoting effect on the yield and quality of hemp, and its various data are better than those of other control groups. From the data of the control groups, it can be seen that only by using the complete bacterial strain combination of the present invention can the best technical effect be achieved. If some of the bacterial strains are discarded, or some of the bacterial strains are replaced with bacterial strains with similar effects, the use effect of the bacterial agent will be affected.

[0094] From the data of control groups 3 and 4, it can be seen that whether the biostimulant is added or the different formulations will affect the effectiveness of the microbial agent, and there is a synergistic effect between konjac glucomannan, versicolor polysaccharide and Pu'er tea polysaccharide. Simply adding a certain component will not exert the desired effect.

[0095] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A biological control bactericide for pests and diseases of cannabis plants, characterized in that, The invention is prepared from the following components in parts by weight: 10-15 parts of Bifidobacterium bifidum, 12-16 parts of Paenibacillus polymyxa, 16-20 parts of Bacillus amyloliquefaciens, 12-16 parts of Burkholderia, 13-17 parts of Pythium oligandrum, 6-10 parts of Lactobacillus plantarum, 15-19 parts of Bacillus velezensis, 15-19 parts of Pseudomonas aeruginosa and 12-16 parts of Metarhizium anisopliae.

2. The biological control bactericide for pests and diseases of cannabis plants according to claim 1, characterized in that, The bacterial contents of the Bifidobacterium bifidum, Paenibacillus polymyxa, Bacillus amyloliquefaciens, Burkholderia, Pythium oligandrum, Lactobacillus plantarum, Bacillus velezensis, Pseudomonas aeruginosa and Metarhizium anisopliae are all 400 million to 700 million per gram.

3. The biological control bactericide for pests and diseases of cannabis plants according to claim 1 or 2, characterized in that, It also includes 2 to 4 parts of biostimulants; the biostimulants are: konjac glucomannan, versicolor polysaccharide and tuckahoe tea polysaccharide; the mass ratio of konjac glucomannan, versicolor polysaccharide and tuckahoe tea polysaccharide is 3 to 5:12 to 16:15 to 25.

4. The preparation method of the biological control fungicide for pests and diseases of cannabis plants according to claim 3, characterized in that, The method comprises mixing Bifidobacterium bifidum, Paenibacillus polymyxa, Bacillus amyloliquefaciens, Burkholderia spp., Pythium oligandrum, Lactobacillus plantarum, Bacillus velezensis, Pseudomonas aeruginosa, Metarhizium anisopliae and biostimulants.

5. Use of the biological control agent for hemp plant diseases and insect pests according to any one of claims 1 to 3 or the biological control agent for hemp plant diseases and insect pests obtained by the preparation method according to claim 4 in the preparation of hemp seed dressings.

6. A cannabis seed dressing agent, characterized in that, The invention comprises 5 to 15 parts of the biological control agent for hemp plant diseases and insect pests according to any one of claims 1 to 3 or the biological control agent for hemp plant diseases and insect pests obtained by the preparation method according to claim 4, 2 to 5 parts of a wetting agent, 2 to 5 parts of a film-forming agent, and 2 to 5 parts of a thickener.

7. The seed dressing agent for cannabis seeds according to claim 6, characterized in that, The wetting agent is sodium dodecyl sulfate, calcium dodecylbenzene sulfonate or sodium methylene dinaphthalene sulfonate; the film-forming agent is sodium carboxymethyl cellulose, polyvinyl alcohol or sodium alginate; and the thickener is xanthan gum, magnesium aluminum silicate or polyvinyl alcohol.

8. A method for preparing a cannabis seed dressing agent according to claim 6 or 7, characterized in that, The hemp plant pest and disease biological control fungus agent, wetting agent, film-forming agent and thickening agent are mixed.

9. A method for using a cannabis seed dressing agent according to claim 6 or 7 or a cannabis seed dressing agent prepared by the preparation method according to claim 8, characterized in that, Mix the hemp seed dressing with water and add the hemp seeds.

10. The usage method according to claim 9, characterized in that, The mass ratio of the hemp seed dressing agent, water and hemp seeds is 1:8-20:800-900.

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