Formula camel milk powder with function of reducing blood fat

Abstract

The invention relates to the technical field of preparation of modified milk powder, and discloses formula camel milk powder with a blood fat reducing function, which comprises the following raw materials: camel milk powder, red yeast rice extract, hawthorn extract and green tea extract. The composition can further comprise raw materials of oat beta-glucan or zinc citrate. Flavones, polyphenols and other plant active ingredients in the extracts of the various medicinal and edible materials have a synergistic effect with rich fatty acids, high-quality proteins, natural antioxidant active substances and the like in the camel milk, so that the blood fat reducing function of the camel milk powder is remarkably improved, the acting time of the blood fat reducing function of the camel milk powder is shortened, and the blood fat reducing dosage of the camel milk powder is reduced; in addition, the selected raw materials are all from extracts of medicinal and edible materials, so that good safety is achieved, and the application prospect is wide.

Description

Technical Field

[0001] The invention relates to the technical field of preparation of modulated milk powder, in particular to a formula camel milk powder with the function of lowering blood lipids. Background Art

[0002] In recent years, chronic non-communicable diseases related to hyperlipidemia have increased and have become an important social problem. The strict definition of hyperlipidemia is dyslipidemia or abnormal blood lipids, which means that the concentration level of serum lipids in the human body exceeds the normal range, including elevated serum total cholesterol (TC) level, elevated serum triglyceride (TG) level, and decreased serum high-density lipoprotein cholesterol (HDL-C) level. The formation of hyperlipidemia involves multiple complex metabolic pathways, mainly including the following aspects: lipid absorption and synthesis, including a high-fat diet that causes a large amount of cholesterol and triglycerides to enter the body, cholesterol and triglycerides are emulsified by bile acid and then absorbed by the small intestine, and the liver uses acetyl-CoA to synthesize cholesterol and triglycerides; lipoprotein metabolism, including chylomicrons (CM) synthesized in the small intestine to transport triglycerides and cholesterol in the diet, very low-density lipoprotein (VLDL) synthesized in the liver to transport endogenous triglycerides, low-density lipoprotein (LDL) produced by VLDL metabolism, mainly transporting cholesterol to peripheral tissues, and high-density lipoprotein (HDL) collecting excess cholesterol from peripheral tissues and transporting it back to the liver for metabolism; and pathological causes, such as abnormal lipid metabolism, inflammation and oxidative stress, and genetic factors.

[0003] Currently, people with hyperlipidemia mainly rely on reducing the intake of lipids and cholesterol in food and increasing exercise to lower blood lipids. When hyperlipidemia reaches the disease standard, statins, cholesterol absorption inhibitors, PCSK9 inhibitors, fibrates, niacin drugs and bile acid sequestrants are needed for treatment. However, there are not many practices of using functional foods to assist in lowering blood lipids, which is mainly limited by the small amount of food taken and the late time to take effect.

[0004] People are paying more and more attention to the prevention and control of hyperlipidemia, and various studies on lowering blood lipids are also being actively carried out. Camel milk is rich in a variety of beneficial ingredients, which may play a role in assisting the regulation of blood lipids. For example, the unsaturated fatty acids it contains, such as linoleic acid and linolenic acid, can help lower low-density lipoprotein cholesterol (LDL-C) and increase high-density lipoprotein cholesterol (HDL-C). The whey protein it contains helps reduce cholesterol absorption and promote metabolism, and the antioxidants it contains, such as vitamins C and E, reduce oxidative stress and protect blood vessels. However, current studies have shown that long-term drinking of camel milk and a low-fat diet are required to be effective, and the overall effect on lowering blood lipids is not significant.

[0005] There is little research on the lipid-lowering effect of formula camel milk. Patent CN201210495278.1 provides a health-care camel milk for preventing and treating hyperlipidemia, which is composed of the following raw materials: dry powder of Chinese medicine fluid extract and sterilized camel milk; the dry powder of Chinese medicine fluid extract is made from milk root, licorice, watermelon peel, dodder seed and bamboo leaves. After making a fluid extract by adding an appropriate amount of water in a certain proportion, it is then frozen and pulverized into dry powder. There is a lack of verification of its biological efficacy for preventing hyperlipidemia, and this patent does not quantitatively record the lipid-lowering function and its onset time, making it difficult to determine its efficacy.

[0006] Therefore, there is an urgent need to combine camel milk and medicine and food homology materials to prepare a brand-new formula camel milk powder with lipid-lowering function, so that the beneficial components in camel milk and medicine and food homology materials can play a synergistic role, expand the application of camel milk in functional foods, and improve its lipid-lowering function. Summary of the Invention

[0007] To solve the above existing problems, the present invention discloses a formula camel milk powder with lipid-lowering function, and finds a brand-new formula. Flavonoids, polyphenols and other plant active ingredients in the extracts of various medicine and food homology materials synergistically act with the rich fatty acids, high-quality proteins and natural antioxidant active substances in camel milk, significantly improving the lipid-lowering function of camel milk powder, shortening the onset time of the lipid-lowering function of camel milk powder, and reducing the dosage of camel milk powder for lipid-lowering. In addition, the raw materials selected in the present invention are all extracts from medicine and food homology materials, with good safety and broad application prospects.

[0008] Currently, hyperlipidemia is mainly judged as a whole based on the serum levels of TG (triglyceride), TC (total cholesterol), HDL-C (high-density lipoprotein cholesterol) and LDL-C (low-density lipoprotein cholesterol); based on the above lipid indexes, the lipid-lowering function of the formula camel milk powder of the present invention has the following meanings: First, individuals with hyperlipidemia, after taking the formula camel milk powder for a period of time, all lipid indexes return to the clinically recognized normal range, and the symptoms of hyperlipidemia are alleviated; Second, individuals with hyperlipidemia take the formula camel milk powder, and compared with taking camel milk or some components in the formula alone, all lipid indexes are closer to the clinically recognized normal range; Third, individuals without hyperlipidemia, after taking the formula camel milk powder for a period of time, the risk of developing hyperlipidemia is reduced; Fourth, individuals without hyperlipidemia take the formula camel milk powder, and compared with taking camel milk or some components in the formula alone, the risk of developing hyperlipidemia is lower; The improvement of the lipid-lowering efficacy of the formula camel milk powder of the present invention has the following meanings: First, the above four lipid-lowering functions are further improved; Second, after individuals take the formula camel milk powder, compared with taking camel milk or some components in the formula, they can reduce all lipid indexes in a shorter time; Third, individuals can achieve equivalent lipid-lowering effects with less quality of the formula camel milk powder.

[0009] On the one hand, the present invention provides a formulated camel milk powder with lipid-lowering function, comprising the following raw materials: camel milk powder, red yeast rice extract, hawthorn extract and green tea extract.

[0010] Camel milk is rich in high-quality protein, unsaturated fatty acids and natural antioxidants, which can reduce blood lipids; red yeast rice contains natural statin substances, which can inhibit cholesterol synthesis and reduce blood lipids; hawthorn is rich in flavonoid compounds, which can promote fat decomposition and reduce triglycerides and cholesterol; green tea is rich in tea polyphenols, which have antioxidant effects and reduce lipid peroxidation; it should be noted that although the above-mentioned camel milk powder and the medicinal and edible homologous materials all have lipid-lowering effects, they only exist in theory, and their use in food supplements for lipid-lowering has not been supported by large-scale clinical data. Even if they play a lipid-lowering role, they usually need to be used in large amounts for a long time, which will also burden the digestive system of patients. Excessive consumption is likely to induce other health problems instead.

[0011] The present invention extracts the active ingredients from the medicinal and edible homologous materials, and through a certain extraction method, extracts as many components as possible that have potential synergistic effects on lipid-lowering, and avoids extracting substances that are harmful to health. In addition, although the above-mentioned several medicinal and edible homologous materials are currently considered to have lipid-lowering effects, the overall synergistic effect they produce with camel milk has no direct connection with the lipid-lowering effects they are individually considered to have. The generation of the synergistic effect is due to the complex mechanisms between substances and between substances and organisms. Since synergy can produce a much higher effect than any one of the components alone, it can be fully considered that the physiological pathways involved in the synergistic effect are different from those involved in the individual components; in addition, regarding the lipid-lowering effects of the medicinal and edible homologous materials, more than thirty medicinal and edible homologous materials that are considered to have lipid-lowering effects were selected in the early stage of the present invention, such as cassia seed, mulberry leaf, lotus leaf, chrysanthemum, wolfberry, kudzu root, poria cocos, black fungus and garlic, etc. However, only the extracts of several medicinal and edible homologous materials obtained by the present invention and the formula of camel milk powder have been experimentally verified to have significant synergistic lipid-lowering effects. Others are just the stacking of effects or ineffective, and even the combination use may increase blood lipids. The reason is that there are complex synergistic and antagonistic mechanisms between plant active ingredients.

[0012] In some methods, the preparation method of the red yeast rice extract is: take red yeast rice and extract it with a polar solvent, filter to remove the residue, vacuum concentrate the filtrate, and dry the concentrated solution into powder.

[0013] Red yeast rice is made from rice such as indica rice, japonica rice, and glutinous rice, and fermented by Monascus purpureus, resulting in brownish-red or purplish-red rice grains. It belongs to the materials that are both medicine and food. Those obtained through regular channels and passing quality inspection are equivalent. In terms of technical principles, the active ingredients in it are mainly produced by Monascus purpureus, so the active ingredients also have equivalence. Therefore, the present invention does not limit the source of the raw materials.

[0014] Preferably, the polar solvent includes an aqueous acetone solution.

[0015] In some ways, the preparation method of the hawthorn extract is as follows: crush the hawthorn fruits into powder, extract the hawthorn fruit powder with a polar solvent, filter to remove the residue, vacuum-concentrate the filtrate, and dry the concentrated solution into powder.

[0016] Similarly, the active ingredients in hawthorn fruits are mainly derived from gene expression and biological metabolism. There are great similarities in the genome and metabolism among different species of hawthorn, so their active ingredients have equivalence. Therefore, the present invention does not limit the source of the raw materials.

[0017] Preferably, the polar solvent includes an aqueous n-butanol solution.

[0018] In some ways, the preparation method of the green tea extract is as follows: crush the green tea leaves into small pieces or powder, extract with hot water, filter to remove the residue, vacuum-concentrate the filtrate, and dry the concentrated solution into powder.

[0019] The green tea leaves mainly include West Lake Longjing, Anji Baihao, Biluochun, etc. The present invention has tested all the main types of green tea leaves and can obtain approximate effects. Due to the similarity of the active ingredients in green tea leaves and the approximation of the test results, the present invention does not limit the source of the raw materials.

[0020] Preferably, the temperature of the hot water is 65 - 85 °C.

[0021] In some ways, the mass ratio of the camel milk powder, red yeast rice extract, hawthorn extract, and green tea extract is 500 - 5000:1 - 10:1 - 10:1 - 10.

[0022] Furthermore, it also includes the raw material: oat β-glucan.

[0023] β-(1→3,1→4)-glucan in oats is abbreviated as oat β-glucan, which is a non-starch polysaccharide present in the cell walls of oat endosperm and aleurone layer. It is a high-molecular polymer formed by monomer β-D-glucopyranose connected by β-(1→3) and β-(1→4) glycosidic bonds. The preparation of oat β-glucan can be extracted from oats or synthesized by high-molecular methods.

[0024] It was accidentally discovered in the present invention that adding oat β-glucan can further shorten the onset time of the above-mentioned formula camel milk powder (calculated according to the blood lipid index reduced to the 60th percentile). Since shortening the onset time mainly lies in improving bioavailability, it is preliminarily speculated that the principle may be to produce a synergistic effect with the relevant active substances in the formula camel milk powder, generating a new physiological pathway for reducing blood lipid. Each physiological pathway proceeds simultaneously, achieving the blood lipid-lowering effect in advance, and the specific mechanism awaits further research.

[0025] In some embodiments, the raw material oat β-glucan accounts for 0.2 - 1.5% by mass.

[0026] Furthermore, it further includes the raw material: zinc citrate.

[0027] Zinc citrate, also known as zinc citrate, is an organic compound with the CAS number: 546 - 46 - 3, and is commonly used as a zinc supplement in functional foods. It was accidentally discovered in the present invention that adding zinc citrate can reduce the dosage of the formula camel milk powder while ensuring the same blood lipid-lowering effect. From a theoretical perspective, its effect on the formula camel milk powder may be that on the one hand, it provides a chelating effect to chelate metal ions such as iron and calcium that are not conducive to reducing blood lipid, which can significantly increase the blood concentration of bioactive molecules in the formula camel milk powder in theory even when the dosage is reduced. On the other hand, it provides zinc, and zinc may play the role of a coordination center to combine relevant active substances together and promote their synergistic effect, and the specific mechanism awaits further research.

[0028] In some embodiments, the raw material zinc citrate accounts for 0.1 - 1.5% by mass.

[0029] On the other hand, the present invention provides the use of a composition for preparing a reagent for enhancing the blood lipid-lowering effect of camel milk powder, and the composition includes red yeast rice extract, hawthorn extract, and green tea extract.

[0030] In some embodiments, enhancing the blood lipid-lowering effect of camel milk powder includes reducing the serum TC or TG level of an individual.

[0031] In some embodiments, enhancing the blood lipid-lowering effect of camel milk powder includes increasing the serum HDL-C level or reducing the serum LDL-C level of an individual.

[0032] On the other hand, the present invention provides the use of an oat β-glucan for preparing a reagent for enhancing the blood lipid-lowering effect of a formula camel milk powder, and the formula camel milk powder includes the following raw materials: camel milk powder, red yeast rice extract, hawthorn extract, and green tea extract.

[0033] In some embodiments, enhancing the blood lipid-lowering effect of the formula camel milk powder includes shortening the onset time of the blood lipid-lowering effect.

[0034] On the other hand, the present invention provides a use of zinc citrate for preparing an agent for improving the lipid-lowering effect of formula camel milk powder, wherein the formula camel milk powder comprises the following raw materials: camel milk powder, red yeast rice extract, hawthorn extract and green tea extract.

[0035] In some embodiments, improving the lipid-lowering effect of camel milk formula includes reducing the amount of camel milk formula taken while maintaining the lipid-lowering effect.

[0036] In summary, the present invention includes at least one of the following beneficial technical effects: 1. The present invention discloses a formula camel milk powder with blood lipid lowering function, and finds a new formula. The auxiliary blood lipid lowering composition and camel milk powder can produce a synergistic effect, significantly improve the blood lipid lowering function of camel milk powder, shorten the time for the blood lipid lowering function of camel milk powder to take effect, and reduce the blood lipid lowering dosage of camel milk powder. In addition, the raw materials selected by the present invention are all extracts from medicinal and edible materials, which have good safety and broad application prospects. 2. The present invention has undergone a lot of theoretical research and experimental verification, and has found a suitable formula from a large number of medicinal and edible materials and food reagents with lipid-lowering and other related functions. The components have a synergistic effect and can return the blood lipid indicators of individuals with hyperlipidemia to the range of healthy individuals; 3. The present invention accidentally discovered that adding oat β-glucan can further shorten the onset time of the above-mentioned formula camel milk powder. The preliminary speculation is that the principle may be synergistic with the relevant active substances in the formula camel milk powder, generating a new physiological pathway for lowering blood lipids. The various physiological pathways are carried out synchronously, and the blood lipid-lowering effect is achieved in advance, which is conducive to the preparation of functional foods for rapid blood lipid lowering; 4. The present invention accidentally discovered that adding zinc citrate can reduce the dosage of camel formula milk powder while ensuring the lipid-lowering effect, which is beneficial to reducing the digestive system burden and other related potential problems of individuals taking camel formula milk powder to lower blood lipids. DETAILED DESCRIPTION

[0037] The present invention is further described in detail below in conjunction with specific examples, which are only used to explain the present invention and are not used to limit the scope of the present invention. The materials, reagents, etc. used in the following examples are reagents and materials available from commercial sources unless otherwise specified. Example 1: Preparation of camel milk powder with lipid-lowering function

[0038] First, fresh camel milk transported from the pasture is taken and filtered using a double filter to remove most of the impurities and microorganisms in the fresh camel milk through filtration and adsorption; then it is purified using a milk purifier to further remove mammary gland cells and certain microorganisms in the fresh camel milk; then it is sent to a spray drying equipment for spray drying to obtain camel milk powder.

[0039] The preparation method of red yeast rice extract is as follows: Take red yeast rice (produced in Shaoxing, Zhejiang), add 20 times the volume of 70% ethanol aqueous solution, place it in an ultrasonic bath and extract at 40°C for 6 hours, filter to remove the residue, vacuum-concentrate the filtrate, and dry the concentrated solution into a powder.

[0040] The preparation method of hawthorn extract is as follows: Crush hawthorn fruits (produced in Qinghe, Hebei) into a powder, add 20 times the volume of 70% ethanol aqueous solution, place it in an ultrasonic bath and extract at 40°C for 6 hours, filter to remove the residue, vacuum-concentrate the filtrate, and dry the concentrated solution into a powder.

[0041] The preparation method of green tea extract is as follows: Crush West Lake Longjing tea leaves into small pieces or powder, add 10 times the volume of water, heat and keep warm at 80°C, extract for 6 hours, filter to remove the residue, vacuum-concentrate the filtrate, and dry the concentrated solution into a powder.

[0042] Send the camel milk powder, red yeast rice extract, hawthorn extract, and green tea extract obtained above into a fluidized bed for homogenization and drying according to a mass ratio of 1000:1:1:1 to obtain a formulated camel milk powder with lipid-lowering function. Example 2: Lipid-lowering efficacy experiment of formulated camel milk powder

[0043] First, prepare camel milk powder, red yeast rice extract, hawthorn extract, and green tea extract according to the method of Example 1, and configure the formulated camel milk powder with the following compositions according to the method of Example 1: Formula 1: Send camel milk powder, red yeast rice extract, hawthorn extract, and green tea extract into a fluidized bed for homogenization and drying according to a mass ratio of 1000:1:1:1 to obtain a formulated camel milk powder with lipid-lowering function; Formula 2: Send sheep milk powder (prepared from fresh sheep milk according to the preparation method of camel milk powder in Example 1), red yeast rice extract, hawthorn extract, and green tea extract into a fluidized bed for homogenization and drying according to a mass ratio of 1000:1:1:1; Formula 3: Send camel milk powder, hawthorn extract, and green tea extract into a fluidized bed for homogenization and drying according to a mass ratio of 1000:1:1; Formula 4: Send camel milk powder, red yeast rice extract, and green tea extract into a fluidized bed for homogenization and drying according to a mass ratio of 1000:1:1; Formula 5: Send camel milk powder, red yeast rice extract, and hawthorn extract into a fluidized bed for homogenization and drying according to a mass ratio of 1000:1:1; Formula 6: Send camel milk powder, cassia seed extract, hawthorn extract, and kudzu root extract (all prepared by adding 20 times the volume of ethanol and extracting with ultrasound at 40°C for 6 h) into a fluidized bed for homogenization and drying according to a mass ratio of 1000:1:1:1.

[0044] To evaluate the lipid-lowering efficacy, the levels of TG, TC, HDL-C, and LDL-C in the serum of rats were measured. Among them, a lower level of TG (triglyceride) indicates normal lipid metabolism, and an elevated level indicates hyperlipidemia. A moderate level of TC (total cholesterol) indicates a balanced cholesterol metabolism, and an elevated level indicates hypercholesterolemia. A higher level of HDL-C (high-density lipoprotein cholesterol) indicates a stronger reverse cholesterol transport ability, and the higher it is within the normal range, the better. A lower level of LDL-C (low-density lipoprotein cholesterol) indicates less cholesterol transport to surrounding tissues, and the lower it is within the normal range, the better.

[0045] The hyperlipidemia model of rats has a high similarity to that of humans. The method of the animal experiment in this example refers to the method in relevant research, as follows: SPF-grade male SD rats (Beijing Vital River Laboratory Animal Technology Co., Ltd.) at 6 weeks of age, with a body weight of 120 ± 20 g, were adaptively fed with a basal diet for 1 week. The feeding temperature was 22 ± 2 °C, the humidity was 40 - 70%, and the light was alternated between light and dark for 12 h. They had free access to food and water. Randomly select 10 rats as the blank group, and intragastrically administer 1 mL / 100 g (body weight) of normal saline daily. The remaining rats were used as the experimental group and were intragastrically administered 1 mL / 100 g (body weight) of a high-fat emulsion daily, once a day for a total of 60 days. At the same time, each group of rats was ensured to have normal access to drinking water and basal diet. Among them, the high-fat emulsion was prepared by mixing sucrose, lard, egg yolk, cholesterol, sodium cholate, and basal diet according to a mass ratio of 10:12:10:1:0.5:66.5. After the last intragastric administration of each group of rats, they were fasted for 12 h without water restriction, and 1.5 mL of blood was collected from the orbital venous plexus to measure TG, TC, HDL-C, and LDL-C to reflect the blood lipid levels of the rats. When there was a significant difference in the blood lipid levels of the experimental group rats compared with the blank group, the model was successfully established. Select the successfully modeled SD rats and randomly divide them into 7 groups, with 10 rats in each group, namely the model group, treatment groups 1 - 6. The treatments for each group are as follows: Blank group: Intragastrically administer normal saline; Model group: Intragastrically administer a high-fat emulsion; Treatment group 1: Intragastrically administer the formulated camel milk powder of formula 1; Treatment group 2: Intragastrically administer the formulated camel milk powder of formula 2; Treatment group 3: Intragastrically administer the formulated camel milk powder of formula 3; Treatment group 4: Intragastrically administer the formulated camel milk powder of formula 4; Treatment group 5: Intragastrically administer the formulated camel milk powder of formula 5; Treatment group 6: Intragastrically administer the formulated camel milk powder of formula 6; Each group of rats was gavaged once every morning, and the gavage volume each time was 0.5 mL (0.5 g of formula camel milk powder for each group). Gavage was continued for 60 days. During the experiment, except for the blank group, each group of rats was given a high-fat emulsion to ensure that the nursing measures and feeding management of each group of rats were exactly the same. After the last gavage of each group of rats, they were fasted for 12 h without water restriction. They were anesthetized intraperitoneally with 10% chloral hydrate at 400 mg / kg (body weight), and 5 mL of whole blood was collected from the abdominal aorta. The blood was allowed to stand at room temperature until it coagulated naturally, and then centrifuged (3500 rpm, 10 min, 4°C). The supernatant was taken as serum, and the total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) in the serum were measured. The mean value was calculated within the group, and the results are shown in Table 1.

[0046] Table 1: Serum lipid levels of rats in each group (n = 10)

[0047] According to the experimental results, compared with the model group, TC, TG, and LDL-C in treatment group 1 were significantly decreased, and HDL-C was significantly increased. All indicators were relatively close to the blank group, indicating that the formula camel milk powder of formula 1 (prepared from Example 1) has a significant lipid-lowering effect. Treatment groups 2 - 5 were formula milks with goat milk replacing camel milk and omitting one of the extracts of medicine and food homology materials, respectively, which had a slight lipid-lowering effect, mainly reducing TC and TG in a small range, and having no obvious effect on HDL-C and LDL-C; Treatment group 6 was a formula camel milk powder in the previous research of the present invention, which instead increased the levels of TG and LDL-C. It may be that the various active ingredients antagonized each other, and thus it did not have a lipid-lowering effect.

[0048] In summary, although various medicine and food homology materials and various milk sources theoretically have a certain lipid-lowering effect, when they are combined, the synergistic or antagonistic effect of the combination far exceeds the effect of a single component. The present invention actually conducts theoretical exploration and experimental verification on a large number of formulas to obtain the formula of camel milk powder, red yeast rice extract, hawthorn extract, and green tea extract. Among them, flavonoids, polyphenols, and other plant active ingredients in the extracts of various medicine and food homology materials synergistically act with the rich fatty acids, high-quality proteins, and natural antioxidant active substances in camel milk, significantly improving the lipid-lowering function of camel milk powder.

[0049] Further proportion experiments showed that the formula camel milk powder obtained by homogenizing camel milk powder, red yeast rice extract, hawthorn extract, and green tea extract according to the mass ratio of 500 - 5000:1 - 10:1 - 10:1 - 10 all had good lipid-lowering effects.

[0050] Example 3: Experiment on the effect of different extraction methods of medicine and food homology materials on the lipid-lowering effect of formula camel milk powder According to the aforementioned experiments, it can be known that there is a synergistic effect among camel milk powder, red yeast rice extract, hawthorn extract and green tea extract. The generation of the synergistic effect is related to the types of active ingredients in the extract. Then, the extraction method is related to the finally synergistically produced lipid-lowering effect. In this embodiment, extracts of medicated and edible homologous materials extracted by different methods in the previous research of the present invention are selected to prepare formula camel milk powder, and a lipid-lowering effect test is carried out.

[0051] Generally speaking, red yeast rice extract needs to contain statins. Existing research has shown that various extraction methods can fully extract statins. Then, it is generally considered that the extraction method has no significant impact on the effect of red yeast rice extract on formula camel milk powder. In this embodiment, the following extraction methods are selected: Method 1, carried out in the manner of Example 1; Method 2, carried out in the manner of Example 1, the difference is that a miscible solution of acetone and water is used for extraction, and the volume ratio of acetone to water is 1:3; Method 3, carried out in the manner of Example 1, the difference is that deionized water is used for extraction. The obtained red yeast rice extracts are respectively used to prepare formula camel milk powder in the manner of Example 1, and the lipid-lowering effect is tested according to the method of Example 2. The test results show that the differences are mainly in the serum concentrations of high-density lipoprotein HDL-C and low-density lipoprotein LDL-C. Therefore, only these two indicators are shown, and the results are shown in Table 2.

[0052] Table 2: Effects of different red yeast rice extraction methods on serum lipid levels of rats (n = 10)

[0053] According to the test results, it can be known that the formula camel milk powder finally obtained by different red yeast rice extraction methods has an obvious impact on the serum concentrations of high-density lipoprotein HDL-C and low-density lipoprotein LDL-C in rats. The most preferred is the miscible solution of acetone and water. When deionized water is used for extraction, the lipid-lowering effect of the formula camel milk powder is reduced.

[0054] From a theoretical perspective, aqueous ethanol solution is the most commonly used extraction solvent because of its high safety and high extraction efficiency. However, it may be difficult to extract some special molecules. Pure water is difficult to extract most organic molecules and mainly extracts water-soluble molecules. The miscible solution of acetone and water may have better extraction efficiency for molecules with special biological activities, and can increase the content of some bioactive molecules with trace amounts. These bioactive molecules with trace amounts may be related to cholesterol metabolism, so that the extraction method is related to HDL-C and LDL-C. From this perspective, it can also be seen that the contribution of red yeast rice extract in formula camel milk powder is not only to provide statins with lipid-lowering effects, but some bioactive molecules with trace amounts may play a more crucial role in lipid-lowering.

[0055] Regarding the hawthorn extract, the present invention also found that selecting a specific solvent for extraction can increase the lipid-lowering effect of the formulated camel milk powder, and this effect is mainly due to the decrease in the serum concentration of triglyceride TG in rats. Using the same method as above for the experiment, the difference in the extraction method for each group lies only in the extraction solvent, and the results are shown in Table 3.

[0056] Table 3: Effects of different hawthorn extraction methods on the serum lipid levels of rats (n = 10)

[0057] According to the experimental results, the formulated camel milk powder prepared from the hawthorn extract obtained by extraction with an aqueous solution of n-butanol can further reduce the serum level of triglyceride in rats and has a higher lipid-lowering effect. From a principle perspective, hawthorn contains a large number of different flavonoid substances and other bioactive molecules. Therefore, selecting acetone water, which has a good extraction effect on red yeast rice, cannot achieve the same good effect, but instead reduces the lipid-lowering effect. Compared with ethanol, the chemical structure of n-butanol is replacing the ethyl group with a n-butyl group. Therefore, due to the possible change in the docking mode with bioactive molecules, the extraction selectivity and extraction rate for various bioactive molecules are changed.

[0058] Regarding the green tea extract, the present invention mainly studied the extraction temperature. The extraction temperature has a significant impact on the serum concentrations of high-density lipoprotein HDL-C and low-density lipoprotein LDL-C in rats by the formulated camel milk powder. Using the same experimental method as above, the experimental results are shown in Table 4.

[0059] Table 4: Effects of different extraction temperatures of green tea extract on the serum lipid levels of rats (n = 10)

[0060] The above only shows the serum concentrations of high-density lipoprotein HDL-C and low-density lipoprotein LDL-C in rats by the formulated camel milk powder finally obtained by green tea extraction at 40 °C, 80 °C, and 100 °C (boiling point). It can be seen that the optimal extraction temperature is 80 °C. Further research shows that good extraction effects can be achieved at 65 - 85 °C. From a principle perspective, temperature affects the biological tissue structure and the activity of bioactive substances in green tea. Therefore, a moderate temperature is better. And catechin substances can be extracted at different extraction temperatures. Therefore, the influence of catechin substances on the serum concentrations of high-density lipoprotein HDL-C and low-density lipoprotein LDL-C in rats may be relatively low, while other trace amounts of bioactive molecules may play a more crucial role. Example 4: Study on the onset time and dosage of the formulated camel milk powder

[0061] In the previous research, the onset time of the lipid-lowering effect was temporarily put aside for discussion because the lipid-lowering effect of the formulated camel milk powder of the present invention after intragastric administration to rats for 60 days was much higher than that of other food supplements for lipid-lowering. Theoretically, even if the intragastric administration time of other food supplements for lipid-lowering was extended, the lipid-lowering effect of the formulated camel milk powder of the present invention could not be achieved because 60 days was sufficient for it to exert sufficient effects. However, one of the major problems with functional foods is that sufficient effects can only be achieved after long-term use. Therefore, in this embodiment, the onset time of the formulated camel milk powder was further discussed. The onset time here refers to reducing the blood lipid level of the rats in the model group to a level close to that of the rats in the blank group and trying to find a method to shorten the onset time. Another major problem with functional foods is the dosage. Usually, a relatively high dosage needs to be taken to achieve good effects, and excessive food intake is not conducive to health. In this embodiment, an attempt was made to find a method to reduce the dosage of the formulated camel milk powder while ensuring the effects.

[0062] Regarding how to test the onset time, first, the rats in the model group and the blank group in Example 2 were taken, and blood was collected from the orbital plexus vein to test the levels of total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) in the serum. The levels of each index in the serum of the blank group rats were set as zero points (0%), and the levels of each index in the serum of the model group rats were set as percentage points (100%). When all the indexes of the rats during the experiment were reduced to 60%, it was regarded as the onset (both were the within-group means, n = 10). To reduce the number of detections, blood was collected for detection every week. When all four indexes were reduced to 60% in one detection, the detection day was recorded as the onset day. When less than four indexes were reduced to 60% in one detection, blood was collected again after 2 days until all four indexes were reduced to 60%, and the onset day was recorded. The onset time was the onset day - the start day of the experiment.

[0063] In fact, most of the experiments in the present invention were carried out in the same batch. The onset time data of Treatment Groups 1-6 in Example 2 are shown in Table 5. Specifically noted that since the HDL-C and LDL-C in Treatment Groups 2-6 did not change significantly, the onset of these two indexes was not considered here.

[0064] Table 5: Onset time data of Treatment Groups 1-6 in Example 2

[0065] According to the above test results, it can be seen that the onset time of Treatment Group 1 (i.e., the formulated camel milk powder prepared in Example 1) was significantly shortened. The TC and TG indexes of the rats were reduced to the 60th percentile after 11 days of intragastric administration, while the other groups did not start to take effect until more than 40 days.

[0066] To further shorten the onset time, the present invention has made a large number of attempts. Some of the formulations with better effects and their onset times are as follows (due to the short onset time, the onset time was tested by taking blood for detection every 3 days. When less than four indicators were reduced to the 60th percentile, the blood sampling interval was changed to 2 days, and the other methods were the same as above): Formulation 7: Based on the formulated camel milk powder prepared in Example 1, an additional 1% by mass of oat β-glucan (Shaanxi Sinuote Biotechnology Co., Ltd.) was added; Formulation 8: Based on the formulated camel milk powder prepared in Example 1, an additional 1% by mass of polydextrose was added; Formulation 9: Based on the formulated camel milk powder prepared in Example 1, an additional 1% by mass of riboflavin was added; The onset times of the above formulations were 6 days, 10 days, and 10 days respectively. Further experiments showed that their final lipid-lowering efficacy was comparable to that of the formulated camel milk powder in Example 1. Based on this result, it can be known that when oat β-glucan is added, the onset time of the lipid-lowering efficacy can be significantly shortened. When polydextrose and riboflavin are added, the onset time is slightly shortened. Therefore, the scheme of adding oat β-glucan is preferred. Since shortening the onset time mainly lies in improving bioavailability, it is preliminarily speculated that the principle may be to produce a synergistic effect with the relevant active substances in the formulated camel milk powder, generating a new physiological pathway for lipid-lowering. Each physiological pathway proceeds simultaneously, achieving the lipid-lowering efficacy in advance, and the specific mechanism awaits further research.

[0067] Further proportional experiments showed that the addition ratio of oat β-glucan is preferably 0.2 - 1.5% of the total mass.

[0068] Regarding how to test the dosage, take the rats in the model group in Example 2, and take the formulated camel milk powder or other functional foods to be tested. The rats in the model group are divided into multiple groups, and the gavage amount of each group is halved compared with the previous group. The other methods are the same as those in Example 2. If there is no significant difference in the various indicators of the rats in the group with the halved gavage amount after 60 days of gavage compared with the previous group, it indicates that the halved dosage can also take effect.

[0069] The present invention has conducted a large number of tests. Among them, it was accidentally found that only one group of formulations can halve the dosage while ensuring the effect. It is the formulated camel milk powder prepared based on Example 1, with an additional 1% by mass of zinc citrate added. When zinc citrate is further replaced with sodium citrate, this effect is not achieved. From a theoretical perspective, its effect on the formulated camel milk powder may be that on the one hand, it provides a chelating effect to chelate metal ions such as iron and calcium that are not conducive to lipid-lowering, which can significantly increase the blood concentration of bioactive molecules in the formulated camel milk powder in theory, even if the dosage is reduced. On the other hand, it provides zinc, and zinc may play the role of a coordination center, combining relevant active substances together to promote their synergistic effect, and the specific mechanism awaits further research.

[0070] Further proportion experiments show that the addition proportion of zinc citrate is preferably 0.1 - 1.5% of the total mass.

[0071] As described above, it is only the specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any various equivalent modifications or substitutions that can be easily thought of by those skilled in the art within the technical scope disclosed by the present invention should be covered within the protection scope of the present invention.

Claims

1. A formulated camel milk powder with blood lipid-lowering function, characterized in that, It includes the following raw materials: camel milk powder, red yeast rice extract, hawthorn extract, and green tea extract.

2. The formula camel milk powder with lipid-lowering function according to claim 1, characterized in that, The preparation method of the red yeast rice extract is as follows: Take red yeast rice and extract it with a polar solvent, filter to remove the residue, vacuum-concentrate the filtrate, and dry the concentrated solution into a powder.

3. The formula camel milk powder with blood lipid-lowering function according to claim 1, characterized in that, The preparation method of the hawthorn extract is as follows: Crush hawthorn fruits into a powder, extract the hawthorn fruit powder with a polar solvent, filter to remove the residue, vacuum-concentrate the filtrate, and dry the concentrated solution into a powder.

4. The formula camel milk powder with lipid-lowering function according to claim 1, characterized in that, The preparation method of the green tea extract is as follows: Crush green tea leaves into small pieces or a powder, extract with hot water, filter to remove the residue, vacuum-concentrate the filtrate, and dry the concentrated solution into a powder.

5. The formula camel milk powder with blood lipid-lowering function according to claim 1, wherein, The mass ratio of the camel milk powder, red yeast rice extract, hawthorn extract, and green tea extract is 500 - 5000:1 - 10:1 - 10:1 - 10.

6. The formula camel milk powder with lipid-lowering function according to claim 1, characterized in that, It also includes the raw material: oat β-glucan.

7. The formula camel milk powder with blood lipid-lowering function according to claim 1, characterized in that It also includes the raw material: zinc citrate.

8. Use of a composition for preparing a reagent for enhancing the lipid-lowering efficacy of camel milk powder, characterized in that, The composition includes red yeast rice extract, hawthorn extract, and green tea extract.

9. Use of an oat β-glucan for preparing a reagent for enhancing the lipid-lowering efficacy of formula camel milk powder, characterized in that, The formulated camel milk powder includes the following raw materials: Camel milk powder, red yeast rice extract, hawthorn extract, and green tea extract.

10. Use of zinc citrate for preparing a reagent for enhancing the lipid-lowering efficacy of formula camel milk powder, characterized in that, The formulated camel milk powder includes the following raw materials: Camel milk powder, red yeast rice extract, hawthorn extract, and green tea extract.

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