Skin-care face cream containing natural extracts and preparation method of skin-care face cream

Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to skin care face cream containing natural extracts and a preparation method. The skin-care face cream containing the natural extracts is prepared from the following components in percentage by weight: 5 to 10 percent of hydrogenated animal oil, 8 to 15 percent of fermented camellia-seed oil, 3 to 6 percent of squalene, 1 to 3 percent of sacha inchi oil, 1 to 2 percent of wild radish oil, 3 to 8 percent of plant extract combination, 2 to 5 percent of silanized kaolin, 0.1 to 0.5 percent of sodium hyaluronate, 1 to 3 percent of cetostearyl alcohol, 3 to 8 percent of glycerol, 0.5 to 1 percent of phenoxyethanol and the balance of water. 0.1-0.5% of vitamin E and the balance of deionized water. A human body sebum structure is simulated through accurate matching of saturated and unsaturated fatty acids, and skin barrier integrity is remarkably improved and transdermal absorption of active ingredients is promoted by combining skin-friendly permeability of squalene and synergistic effect of active ingredients of vegetable oil.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and particularly relates to a skin care cream containing natural extracts and a preparation method thereof. Background Art

[0002] With the changes in environmental pollution and lifestyle, problems such as sensitivity and dryness caused by damaged skin barrier function have become increasingly prominent. Traditional skin care products mostly use synthetic lipids such as ceramides and cholesterol to simulate the sebum structure, but their chemical sources have potential irritation, and the fatty acid spectrum matching degree with human natural sebum is low, resulting in limited repair effects. Existing natural vegetable oils, such as olive oil and evening primrose oil, although gentle, have an imbalance in the ratio of saturated and unsaturated fatty acids in a single oil phase, are prone to oxidative rancidity (peroxide value > 15 meq / kg), and lack penetration-promoting components, with insufficient transdermal efficiency of active ingredients. In addition, plant extracts mostly focus on single efficacy and are difficult to achieve multi-target synergy such as anti-inflammatory, sunscreen, and whitening.

[0003] Although current fermentation technology can partially improve the activity of oils, the content of key components such as γ-linolenic acid in the products is low (≤8%), and the residual phospholipids and free fatty acids result in a high acid value (≥1.2 mg KOH / g), which requires subsequent refining steps, increasing costs and destroying natural activity. Therefore, there is an urgent need to develop a skin care system with high biomimetic degree, strong stability and multi-effect synergy, and break through the bottleneck of "activity - purity - safety" that is difficult to balance in the existing technology through innovative compounding and process optimization. Summary of the Invention

[0004] In view of the above deficiencies, the purpose of the present invention is to provide a skin care cream containing natural extracts and a preparation method thereof, which can improve the skin repair effect and simultaneously take into account the functions of whitening, moisturizing, antioxidant and sunscreen.

[0005] The present invention provides a skin care cream containing natural extracts, which comprises the following components in percentage by weight: 5 - 10% of hydrogenated animal oil, 8 - 15% of fermented camellia seed oil, 3 - 6% of squalene, 1 - 3% of Sacha Inchi oil, 1 - 2% of wild radish oil, 3 - 8% of plant extract combination, 2 - 5% of silylated kaolin, 0.1 - 0.5% of sodium hyaluronate, 1 - 3% of cetearyl alcohol, 3 - 8% of glycerol, 0.5 - 1% of phenoxyethanol, 0.1 - 0.5% of vitamin E, and the balance is deionized water;

[0006] The fermented camellia seed oil is extracted by mixed fermentation of Bacillus amyloliquefaciens, Lactobacillus plantarum and Wickerhamomyces anomalus; the plant extract combination is composed of platycodon grandiflorum extract, clove extract, pomegranate peel extract, prunella vulgaris extract and astragalus membranaceus extract in a mass ratio of 1 - 2:0.5 - 1:1 - 3:0.5 - 2:2 - 4.

[0007] Preferably, the hydrogenated animal oil is hydrogenated lard with an iodine value of 30 - 50.

[0008] Preferably, the fermented camellia seed oil contains ≥12% γ-linolenic acid and has an acid value ≤0.5mgKOH / g.

[0009] Preferably, the particle size of the silylated kaolin is 1 - 5μm and its oil absorption value is 80 - 120mg / g.

[0010] The present invention also provides a method for preparing the skin care cream, comprising the following steps:

[0011] 1) Preparation of fermented camellia seed oil: Mix cold-pressed camellia seed oil and deionized water at a mass ratio of 1:0.5, add 0.2% Tween80, inoculate with a mixed bacterial solution of Bacillus amyloliquefaciens, Lactobacillus plantarum and Williopsis californica, with a final concentration of 1×10 7 CFU / mL, ferment in a constant temperature shaker at 37°C for 48 hours, after fermentation, centrifuge to separate the oil phase, and filter and sterilize;

[0012] 2) Preparation of the oil phase: Mix and stir the hydrogenated animal oil, fermented camellia seed oil, squalene, Sacha inchi oil and wild radish oil at 75°C;

[0013] 3) Preparation of the water phase: Mix and stir phenoxyethanol, vitamin E, silylated kaolin, glycerol, deionized water, sodium hyaluronate and cetearyl alcohol at 60°C;

[0014] 4) Emulsification and homogenization: Slowly add the oil phase to the water phase, homogenize and shear, perform high-pressure homogenization cycling 2 times, cool to 35°C, add the plant extract combination, stir evenly, and cool to room temperature to obtain.

[0015] Preferably, in step 1), the mass ratio of Bacillus amyloliquefaciens, Lactobacillus plantarum and Williopsis californica strains is 4 - 6:2 - 4:1.

[0016] Preferably, in step 1), it operates at a rotation speed of 150rpm and a dissolved oxygen condition of 30 - 50% for 0 - 12 hours, and at a rotation speed of 50rpm and a dissolved oxygen condition of 1 - 10% for 12 - 48 hours.

[0017] Preferably, in step 4), the homogenization and shearing rotation speed is 6000 - 8000rpm, and the high-pressure homogenization pressure is 17000 - 19000PSI.

[0018] Compared with the prior art, the present invention has at least the following beneficial effects:

[0019] 1) Hydrogenated animal oil, squalene, fermented camellia seed oil, Sacha inchi oil and wild radish oil simulate the human sebum structure through the precise ratio of saturated and unsaturated fatty acids. Combining the skin-friendly permeability of squalene and the active ingredients of vegetable oils, they significantly improve the integrity of the skin barrier and promote the transdermal absorption of active ingredients.

[0020] 2) The combination of Bacillus amyloliquefaciens, Lactobacillus plantarum and Wickerhamomyces anomalus is fermented, and through a staged fermentation process, the content of active ingredients in camellia seed oil is significantly increased and impurities are reduced, while the antioxidant stability is enhanced.

[0021] 3) Add platycodon grandiflorum extract, clove extract, pomegranate peel extract, prunella vulgaris extract and astragalus membranaceus extract to achieve the precise compatibility of five plant ingredients with anti-inflammatory, sunscreen, antibacterial, whitening and moisturizing effects, realize the synergistic skin care effect, and the natural source ingredients reduce the irritation to the skin and reduce allergies. Detailed implementation mode

[0027] In order to make the technical solutions described in the present invention clearer and more understandable to those skilled in the art, the following examples are listed for illustration. It should be noted that the following examples do not limit the scope of protection required by the present invention. The experimental reagents and instruments designed in the examples and comparative examples of the present invention are all common ordinary reagents and instruments unless otherwise specified, and can be obtained from commercial channels. In the examples and comparative examples, the experimental methods used are all conventional methods unless otherwise specified. The Bacillus amyloliquefaciens described in the present invention is purchased from the General Microbiology Center of the China Center for Type Culture Collection, and the preservation number is CICC20164; the Lactobacillus plantarum is purchased from the General Microbiology Center of the China Center for Type Culture Collection, and the preservation number is CICC 21830; the Wickerhamomyces anomalus is purchased from the General Microbiology Center of the China Center for Type Culture Collection, and the preservation number is CICC 32897.

[0028] Example 1

[0029] A skin care cream provided in this example includes the following components in weight percentage: hydrogenated lard 5% (iodine value 30), fermented camellia seed oil 8% (γ-linolenic acid 12.3%), squalene 3%, Sacha inchi oil 1%, wild radish oil 1%, plant extract combination 3% (platycodon grandiflorum: clove: pomegranate peel: prunella vulgaris: astragalus membranaceus = 1:0.5:1:0.5:2), silanized kaolin 2% (particle size 5μm, oil absorption value 80mg / g), sodium hyaluronate 0.1%, cetearyl alcohol 1%, glycerol 3%, phenoxyethanol 0.5%, vitamin E 0.1%, appropriate amount of deionized water.

[0030] Preparation method:

[0031] 1) Preparation of fermented camellia seed oil: Mix cold-pressed camellia seed oil and deionized water at a mass ratio of 1:0.5, add 0.2% Tween80, inoculate with a mixed bacterial solution of Bacillus amyloliquefaciens, Lactobacillus plantarum and Saccharomyces willianus. The mass ratio of Bacillus amyloliquefaciens, Lactobacillus plantarum and Saccharomyces willianus is 4:1:1, and the final concentration is 1×10 7 CFU / mL, ferment in a constant temperature shaker at 37°C for 48 hours. Operate at a rotation speed of 150 rpm and a dissolved oxygen condition of 30% for 0 - 12 hours, and operate at a rotation speed of 50 rpm and a dissolved oxygen condition of 10% for 12 - 48 hours. After fermentation, centrifuge to separate the oil phase and filter and sterilize;

[0032] 2) Preparation of oil phase: Mix hydrogenated animal oil, fermented camellia seed oil, squalene, Sacha inchi oil and wild radish oil at 75°C with stirring;

[0033] 3) Preparation of water phase: Mix phenoxyethanol, vitamin E, silylated kaolin, glycerol, deionized water, sodium hyaluronate and cetearyl alcohol at 60°C with stirring;

[0034] 4) Emulsification and homogenization: Slowly add the oil phase to the water phase, homogenize and shear, perform high-pressure homogenization circulation 2 times, the homogenization and shear rotation speed is 6000 rpm, the high-pressure homogenization pressure is 17000 PSI, cool down to 35°C, add the plant extract combination, stir evenly, and cool to room temperature to obtain.

[0035] Example 2

[0036] A skin care cream provided in this example includes the following components in weight percentage: hydrogenated lard 8% (iodine value 40), fermented camellia seed oil 12% (γ-linolenic acid 14.8%), squalene 4.5%, Sacha inchi oil 2%, wild radish oil 1.5%, plant extract combination 5% (platycodon grandiflorum: clove: pomegranate peel: prunella vulgaris: astragalus membranaceus = 1.5:0.8:2:1:3), silylated kaolin 3% (particle size 3μm, oil absorption value 100mg / g), sodium hyaluronate 0.3%, cetearyl alcohol 2%, glycerol 5%, phenoxyethanol 0.8%, vitamin E 0.3%, and appropriate amount of deionized water.

[0037] Preparation method:

[0038] 1) Preparation of fermented camellia seed oil: Mix cold-pressed camellia seed oil and deionized water at a mass ratio of 1:0.5, add 0.2% Tween80, inoculate with a mixed bacterial solution of Bacillus amyloliquefaciens, Lactobacillus plantarum and Saccharomyces willianus. The mass ratio of Bacillus amyloliquefaciens, Lactobacillus plantarum and Saccharomyces willianus is 5:3:1, and the final concentration is 1×10 7CFU / mL, ferment at 37°C in a constant temperature shaker for 48 hours. Operate at 150 rpm and 40% dissolved oxygen for 0 - 12 hours, and at 50 rpm and 5% dissolved oxygen for 12 - 48 hours. After fermentation, centrifuge to separate the oil phase and filter sterilize;

[0039] 2) Preparation of oil phase: Mix and stir hydrogenated animal oil, fermented camellia seed oil, squalene, Sacha inchi oil, and wild radish oil at 75°C;

[0040] 3) Preparation of water phase: Mix and stir phenoxyethanol, vitamin E, silylated kaolin, glycerol, deionized water, sodium hyaluronate, and cetearyl alcohol at 60°C;

[0041] 4) Emulsification and homogenization: Slowly add the oil phase to the water phase, homogenize and shear, perform high-pressure homogenization cycle 2 times, with a homogenization and shear speed of 7000 rpm and a high-pressure homogenization pressure of 18000 PSI. Cool to 35°C and add the plant extract combination, stir evenly, and cool to room temperature to obtain.

[0042] Example 3

[0043] A skin care cream provided in this example includes the following components by weight percentage: hydrogenated lard 10% (iodine value 50), fermented camellia seed oil 15% (γ-linolenic acid 16.5%), squalene 6%, Sacha inchi oil 3%, wild radish oil 2%, plant extract combination 8% (platycodon: clove: pomegranate peel: prunella vulgaris: astragalus membranaceus = 2:1:3:2:4), silylated kaolin 5% (particle size 1μm, oil absorption value 120mg / g), sodium hyaluronate 0.5%, cetearyl alcohol 3%, glycerol 8%, phenoxyethanol 1%, vitamin E 0.5%, and appropriate amount of deionized water.

[0044] Preparation method:

[0045] 1) Preparation of fermented camellia seed oil: Mix cold-pressed camellia seed oil and deionized water at a mass ratio of 1:0.5, add 0.2% Tween 80, inoculate with a mixed bacterial solution of Bacillus amyloliquefaciens, Lactobacillus plantarum, and Wickerhamomyces anomalus. The mass ratio of Bacillus amyloliquefaciens, Lactobacillus plantarum, and Wickerhamomyces anomalus strains is 6:4:1, and the final concentration is 1×10 7 CFU / mL, ferment at 37°C in a constant temperature shaker for 48 hours. Operate at 150 rpm and 50% dissolved oxygen for 0 - 12 hours, and at 50 rpm and 1% dissolved oxygen for 12 - 48 hours. After fermentation, centrifuge to separate the oil phase and filter sterilize;

[0046] 2) Preparation of oil phase: Mix and stir hydrogenated animal oil, fermented camellia seed oil, squalene, Sacha inchi oil, and wild radish oil at 75°C;

[0047] 3) Preparation of the aqueous phase: Mix phenoxyethanol, vitamin E, silylated kaolin, glycerol, deionized water, sodium hyaluronate and cetearyl alcohol at 60 °C with stirring;

[0048] 4) Emulsification and homogenization: Slowly add the oil phase to the aqueous phase, homogenize and shear, perform high-pressure homogenization cycling 2 times, with a homogenization and shear speed of 8000 rpm and a high-pressure homogenization pressure of 19000 PSI. Cool to 35 °C and add the plant extract combination, stir evenly, and cool to room temperature to obtain the product.

[0049] Comparative Example 1

[0050] Compared with Example 2, the only difference is that hydrogenated lard is replaced with olive oil, and the mass content remains unchanged.

[0051] Comparative Example 2

[0052] Compared with Example 2, the only difference is that fermented camellia seed oil is replaced with camellia seed oil, and the mass content remains unchanged.

[0053] Comparative Example 3

[0054] Compared with Example 2, the only difference is that squalene is replaced with hydrogenated lard, and the mass content remains unchanged.

[0055] Comparative Example 4

[0056] Compared with Example 2, the only difference is that Sacha inchi oil and wild radish oil are replaced with an equal amount of fermented camellia seed oil, and the mass content remains unchanged.

[0057] Comparative Example 5

[0058] Compared with Example 2, the only difference is that platycodon grandiflorum extract is replaced with an equal amount of deionized water, and the mass content remains unchanged.

[0059] Comparative Example 6

[0060] Compared with Example 2, the only difference is that clove extract is replaced with an equal amount of deionized water, and the mass content remains unchanged.

[0061] Comparative Example 7

[0062] Compared with Example 2, the only difference is that pomegranate peel extract is replaced with an equal amount of deionized water, and the mass content remains unchanged.

[0063] Comparative Example 8

[0064] Compared with Example 2, the only difference is that prunella vulgaris extract is replaced with an equal amount of deionized water, and the mass content remains unchanged.

[0065] Comparative Example 9

[0066] Compared with Example 2, the only difference is that astragalus membranaceus extract is replaced with an equal amount of deionized water, and the mass content remains unchanged.

[0067] Comparative Example 10

[0068] Compared with Example 2, the only difference is that the mass ratio of Bacillus amyloliquefaciens, Lactobacillus plantarum and Saccharomyces cerevisiae is 1:1:1, and the mass content remains unchanged.

[0069] Effect experiment test

[0070] 1. Human patch test

[0071] Select 120 healthy adults (half male and half female), aged 18 - 45 years old, divided into 12 groups, without a history of skin diseases and non-sensitive skin. Mark 3 areas of 2×2 cm on the upper back, and apply 2 mg / cm 2 sample to each area, cover with a Finn chamber, and apply occlusively for 48 hours. Observe at 0.5 hour, 24 hours, 48 hours, and 72 hours after removal.

[0072] Score according to the "Technical Specifications for Cosmetics Safety". 0 point: no reaction; 1 point: slight erythema (indistinct border); 2 point: moderate erythema (distinct border); 3 point: severe erythema with mild edema; 4 point: purplish red erythema with blisters or erosion. The results are shown in Table 1.

[0073] Table 1

[0074]

[0075] As can be seen from Table 1, both the examples and the control examples are non-irritating and can be used safely.

[0076] 2. Skin barrier function test

[0077] Test the moisture content of the cheek skin stratum corneum of the creams prepared in Examples 1 - 3 and Comparative Examples 1 - 9. The test instrument is an ASA-M100 skin stratum corneum moisture content tester. The specific test method is as follows: Select 15 healthy women (aged 18 - 45 years old) for each group of creams for trial use. Apply the cream to the marked area on the inner side of the left forearm, and use the other side as a blank control (without applying any product). The subjects apply the sample morning and evening every day for 28 days. First, use the moisture content tester to measure 3 times in the marked area and take the average value as the initial moisture content (T0). Repair index: RI = (T1 moisture content - T0 moisture content) / T0 moisture content × 100%, where T1 moisture content is the value measured by the moisture content tester on the 28th day. Conduct parallel tests 3 times, take the average value, and record the stratum corneum moisture content value. The results are shown in Table 2.

[0078] Table 2

[0079]

[0080] As can be seen from Table 2, compared with the comparative examples, the examples of the present invention can effectively repair the cell barrier and have a good moisturizing effect. In Comparative Examples 1-4, the effect is the worst, indicating that hydrogenated animal oil, squalene, fermented camellia seed oil, sacha inchi oil and wild radish oil can well simulate the structure of human sebum, enhance the penetration of nutritional components and promote absorption.

[0081] 3. DPPH Free Radical Scavenging Test

[0082] Weigh 0.5 g of the cream, add 10 mL of absolute ethanol, ultrasonically bath at 60 °C for 30 minutes, centrifuge, and take the supernatant for standby. Dilute the supernatant to a concentration of 2% wt. Precisely weigh 3.94 mg of DPPH, make up the volume to 100 mL with absolute ethanol to prepare a 0.1 mmol solution. Experimental grouping: blank group (2 mL of absolute ethanol + 2 mL of absolute ethanol), control group (2 mL of DPPH solution + 2 mL of absolute ethanol), sample group (2 mL of DPPH solution + 2 mL of sample solutions with different concentrations). Use an ultraviolet spectrophotometer to measure the absorbance value (A) of each group at a wavelength of 517 nm. The scavenging rate (%) = [1 - (A_sample - A_blank) / A_control] × 100%. The results are shown in Table 3.

[0083] Table 3

[0084]

[0085]

[0086] Note: P < 0.05.

[0087] As can be seen from Table 3, the cream provided by the examples has a stronger effect on free radical scavenging rate than the comparative examples. The effect of Comparative Example 2 is weaker than that of the examples, indicating that the fermented camellia seed oil prepared by the preparation process of the present invention has higher antioxidant performance than ordinary camellia seed oil.

[0088] 4. Cell Scratch Test

[0089] Seed HaCaT cells into a 6-well plate at a density of 5×10 5 cells / mL and culture for 24 hours until confluent. Use a 200 μL sterile pipette tip to vertically contact the cell layer and gently draw 3 parallel straight scratches (with an interval of 5 mm). Gently rinse 3 times with PBS to remove the detached cells. Add a cell culture medium containing 1% (w / w) of the sample, and place the treated cells in a CO2 incubator. Take pictures of the scratched area under a fluorescence microscope at 0 hour, 12 hours, 24 hours, and 36 hours respectively, and calculate the scratched area (St) at each time point. The migration rate (%) = (1 - S t / S0) × 100%, where S0 is the scratched area at 0 hour. Set the blank control group as a serum-free control group. The results are shown in Table 4.

[0090] Table 4

[0091]

[0092] As can be seen from Table 4, the effects of the examples are all better than those of the comparative examples, indicating that the present invention can more effectively improve cell migration and repair ability. The compounding of hydrogenated animal oil, squalene, fermented camellia seed oil, sacha inchi oil and wild radish oil and the synergistic effect with plant extracts improve the repair efficacy of the cream.

[0093] 5. Inhibitory effect on tyrosinase activity

[0094] Weigh 1 g of the cream, add 10 mL of PBS (pH 6.8), ultrasonicate in a water bath at 60 °C for 30 minutes, centrifuge, and filter the supernatant through a 0.22 μm filter membrane to obtain a 10% stock solution. Dilute it to a test concentration of 1% (v / v). Grouping: blank group: 100 μL of PBS + 50 μL of PBS; control group: 100 μL of tyrosinase (50 U / mL) + 50 μL of PBS; sample group: 100 μL of tyrosinase + 50 μL of sample solution with different concentrations. Mix tyrosinase with the sample solution or the control solution, incubate at 37 °C for 10 minutes, add 50 μL of L-tyrosine (final concentration 0.5 mmol) to each well, mix well and immediately place it in a spectrophotometer. Record the absorbance value every 15 minutes at a wavelength of 475 nm for 30 minutes. Inhibition rate (%) = (1 - ΔA 样品 / ΔA 对照 ) × 100%. The results are shown in Table 5.

[0095] Table 5

[0096]

[0097] As can be seen from Table 5, the inhibitory effect of the examples on tyrosinase activity is stronger than that of the comparative examples. In comparative examples 5 - 9, a group of plant extracts is missing, resulting in a decrease in the inhibitory effect on tyrosinase activity.

[0098] The above are the preferred embodiments of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle described in the present invention, several improvements and refinements can be made, and these improvements and refinements should also be regarded as the protection scope of the present invention.

Claims

1. A skin care cream containing natural extracts, characterized in that, It comprises components in the following weight percentages: 5-10% of hydrogenated animal oil, 8-15% of fermented camellia seed oil, 3-6% of squalene, 1-3% of sacha inchi oil, 1-2% of wild radish oil, 3-8% of plant extract combination, 2-5% of silylated kaolin, 0.1-0.5% of sodium hyaluronate, 1-3% of cetearyl alcohol, 3-8% of glycerol, 0.5-1% of phenoxyethanol, 0.1-0.5% of vitamin E, and the balance being deionized water; The fermented camellia seed oil is extracted by mixed fermentation of Bacillus amyloliquefaciens, Lactobacillus plantarum and Williopsis californica; the plant extract combination is composed of platycodon grandiflorum extract, clove extract, pomegranate peel extract, prunella vulgaris extract and astragalus membranaceus extract in a mass ratio of 1-2:0.5-1:1-3:0.5-2:2-4.

2. The skin care cream according to claim 1, characterized in that, The hydrogenated animal oil is hydrogenated lard with an iodine value of 30-50.

3. The skin care cream according to claim 1, wherein The fermented camellia seed oil contains ≥12% of γ-linolenic acid and an acid value ≤0.5mgKOH / g.

4. The skin care cream according to claim 1, characterized in that, The particle size of the silylated kaolin is 1-5μm and the oil absorption value is 80-120mg / g.

5. A method for preparing the skin care cream according to any one of claims 1-4, characterized in that, It includes the following steps: 1) Preparation of fermented camellia seed oil: Mix cold-pressed camellia seed oil and deionized water at a mass ratio of 1:0.5, add 0.2% Tween 80, inoculate with a mixed bacterial solution of Bacillus amyloliquefaciens, Lactobacillus plantarum and Saccharomyces cerevisiae, and the final concentration is 1×10 7 CFU / mL, ferment in a constant temperature shaker at 37°C for 48 hours, after fermentation, centrifuge to separate the oil phase, and filter and sterilize; 2) Preparation of the oil phase: Mix and stir hydrogenated animal oil, fermented camellia seed oil, squalene, sacha inchi oil and wild radish oil at 75°C; 3) Preparation of the water phase: Mix and stir phenoxyethanol, vitamin E, silylated kaolin, glycerol, deionized water, sodium hyaluronate and cetearyl alcohol at 60°C; 4) Emulsification and homogenization: Slowly add the oil phase to the water phase, homogenize and shear, perform high-pressure homogenization circulation twice, cool to 35°C, add the plant extract combination, stir evenly, and cool to room temperature to obtain the product.

6. The preparation method according to claim 5, wherein In the step 1), the mass ratio of Bacillus amyloliquefaciens, Lactobacillus plantarum and Williopsis californica strains is 4-6:2-4:

1.

7. The preparation method according to claim 5, characterized in that, In the step 1), it operates at a rotation speed of 150rpm and a dissolved oxygen condition of 30-50% for 0-12 hours, and at a rotation speed of 50rpm and a dissolved oxygen condition of 1-10% for 12-48 hours.

8. The preparation method according to claim 5, characterized in that, In the step 4), the homogenization and shear rotation speed is 6000-8000rpm, and the high-pressure homogenization pressure is 17000-19000PSI.

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