Tea-peptide composition with soothing and anti-wrinkle effects as well as preparation method and application of tea-peptide composition

Abstract

The invention relates to a tea-peptide composition with soothing and anti-wrinkle effects as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. The invention provides a tea-peptide composition. The tea-peptide composition comprises Keemun black tea fermentation filtrate, DNA sodium and decapeptide-4, the mass ratio of the Keemun black tea fermentation filtrate to the DNA sodium to the decapeptide-4 is that the Keemun black tea fermentation filtrate to the DNA sodium to the decapeptide-4 is equal to (500-10000) to 1 to (1-20). Based on the inflammation inhibition mechanism of the Keemun black tea fermentation filtrate and the DNA sodium and the anti-wrinkle function of decapeptide-4, the soothing-anti-wrinkle dual skin care synergistic interaction is realized. Meanwhile, the experimental research shows that the tea-peptide composition has soothing and anti-wrinkle effects due to a unique synergistic mechanism formed by the Keemun black tea fermentation filtrate, the DNA sodium and the decapeptide-4.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a tea-peptide composition with soothing and anti-wrinkle effects, and a preparation method and application thereof. Background Art

[0002] In recent years, with the increase of environmental pollution, ultraviolet radiation and life pressure, sensitive problems caused by damaged skin barrier and wrinkles caused by collagen loss have become the two core challenges of modern skin health. Currently, the anti-wrinkle products on the market mostly rely on active ingredients such as retinol derivatives and vitamin C. Although they have a certain effect in promoting collagen regeneration, they are prone to cause skin irritation, dryness and desquamation, and are difficult to meet the needs of people with sensitive skin; and the formula for soothing and repairing can improve redness and itching, but lacks the anti-aging mechanism for remodeling the dermis structure.

[0003] In addition, the prior art often achieves dual effects by simply compounding anti-inflammatory ingredients (such as dipotassium glycyrrhizinate) and anti-wrinkle peptides (such as palmitoyl tripeptide-5), but the two types of ingredients have significant contradictions in transdermal absorption efficiency, pH compatibility and long-term stability, resulting in a high activity loss rate in actual application. Therefore, developing a safe composition that has both efficient soothing and deep anti-wrinkle activity, and the ingredients are synergistically stable and suitable for sensitive skin is a technical bottleneck that needs to be broken through in this field. Summary of the invention

[0004] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a tea-peptide composition and a soothing and anti-wrinkle essence with soothing and anti-wrinkle effects, as well as a preparation method and application thereof.

[0005] To achieve the above object, the technical solution adopted by the present invention is:

[0006] In a first aspect, the present invention provides a tea-peptide composition, comprising Keemun black tea fermentation filtrate, sodium DNA and decapeptide-4; the mass ratio of the Keemun black tea fermentation filtrate, sodium DNA and decapeptide-4 is Keemun black tea fermentation filtrate: sodium DNA: decapeptide-4 = (500-10000): 1: (1-20).

[0007] Keemun black tea fermentation filtrate is a product prepared by fermenting Keemun black tea with lactobacillus and Thermus thermophilus. Compared with common green fermentation filtrate, Keemun black tea fermentation filtrate contains higher levels of tea polysaccharides, theabrownins, amino acids, polysaccharides and vitamins, which is beneficial to improving the moisturizing and anti-inflammatory effects of the skin. At the same time, there are differences in active ingredients in tea fermentation filtrates prepared from different types of black tea. Different types of black tea are affected by factors such as region, tea fermentation process, and picking period, resulting in differences in the types and contents of active ingredients in the tea itself, which in turn affects the efficacy of the tea fermentation filtrate.

[0008] Sodium DNA, also known as PDRN, is a polydeoxyribonucleotide with various biological activities, mainly extracted from fish germ cells, especially sperm cells of salmon or trout. Sodium DNA has good tissue repair efficacy, and its immunogenicity and toxicity are relatively low. Therefore, it shows great application potential in tissue repair such as skin wound repair, corneal injury, digestive tract injury repair, and bone injury repair. At the same time, sodium DNA activates adenosine receptor A2A, binds to pro-inflammatory cytokines, and simultaneously stimulates the production of anti-inflammatory cytokines, thereby having anti-inflammatory efficacy and promoting healing and reducing possible swelling.

[0009] Decapeptide-4 (INCI: DECAPEPTIDE-4) is a synthetic peptide composed of arginine, aspartic acid, cysteine, glutamic acid, leucine, methionine, and tyrosine, which has the effect of skin conditioning. As a messenger peptide, decapeptide-4 can penetrate the dermis layer, stimulate the synthesis of collagen and elastin, thereby reversing the skin aging process. It can stimulate the synthesis and development of collagen (type I, type II, type IV) and elastin, increase the water content and water-locking ability of the skin, improve the skin thickness, and reduce fine lines.

[0010] Based on the anti-inflammatory mechanism of Qimen black tea fermentation filtrate and sodium DNA, as well as the anti-wrinkle function of decapeptide-4, the present invention realizes the synergistic effect of soothing and anti-wrinkle dual skin care. At the same time, the experimental research of the present invention shows that Qimen black tea fermentation filtrate and sodium DNA can synergistically improve the elastase inhibition rate of decapeptide-4. Conversely, decapeptide-4 can also synergistically improve the hyaluronidase inhibition rate of Qimen black tea fermentation filtrate and sodium DNA. The unique synergistic mechanism formed by the three makes the tea-peptide composition have both soothing and anti-wrinkle effects.

[0011] As a preferred embodiment of the tea-peptide composition of the present invention, the mass ratio of Qimen black tea fermentation filtrate, sodium DNA, and decapeptide-4 in the tea-peptide composition is Qimen black tea fermentation filtrate: sodium DNA: decapeptide-4 = (1000 - 5000): 1: (2 - 10).

[0012] As a preferred embodiment of the tea-peptide composition of the present invention, the mass ratio of Qimen black tea fermentation filtrate, sodium DNA, and decapeptide-4 in the tea-peptide composition is Qimen black tea fermentation filtrate: sodium DNA: decapeptide-4 = (2000 - 4000): 1: (4 - 8).

[0013] As a preferred embodiment of the tea-peptide composition of the present invention, the mass ratio of Qimen black tea fermentation filtrate, sodium DNA, and decapeptide-4 in the tea-peptide composition is Qimen black tea fermentation filtrate: sodium DNA: decapeptide-4 = 2500: 1: 5.

[0014] Second aspect, the present invention provides the application of the above tea-peptide composition in the preparation of cosmetics.

[0015] As a preferred embodiment of the application of the present invention, the cosmetic dosage form includes but is not limited to at least one of creams, lotions, aqueous solutions, gels, oils, powders, bulk powders or solids, patches, films and lyophilized products.

[0016] Third aspect, the present invention provides a soothing anti-wrinkle essence, comprising the following components in parts by mass: 5-11 parts of the above tea-peptide composition, 0.2-0.6 parts of a thickening agent, 5-9 parts of a humectant, 0.3-0.5 parts of a preservative, and 79-90 parts of water.

[0017] As a preferred embodiment of the soothing anti-wrinkle essence of the present invention, it comprises the following components in parts by mass: 6-9 parts of the above tea-peptide composition, 0.25-0.5 parts of a thickening agent, 6-8 parts of a humectant, 0.3-0.5 parts of a preservative, and 82-88 parts of water.

[0018] As a preferred embodiment of the soothing anti-wrinkle essence of the present invention, it comprises the following components in parts by mass: 8 parts of the above tea-peptide composition, 0.4 parts of a thickening agent, 6 parts of a humectant, 0.4 parts of a preservative, and 85.2 parts of water.

[0019] As a preferred embodiment of the soothing anti-wrinkle essence of the present invention, the thickening agent in the soothing anti-wrinkle essence includes at least one of sodium hyaluronate, carbomer and sodium alginate.

[0020] As a preferred embodiment of the soothing anti-wrinkle essence of the present invention, the thickening agent includes sodium hyaluronate and carbomer, and the mass ratio of sodium hyaluronate to carbomer in the thickening agent is sodium hyaluronate:carbomer = (0.5-3):1.

[0021] As a preferred embodiment of the soothing anti-wrinkle essence of the present invention, the thickening agent includes sodium hyaluronate and carbomer, and the mass ratio of sodium hyaluronate to carbomer in the thickening agent is sodium hyaluronate:carbomer = (1-2):1.

[0022] As a preferred embodiment of the soothing anti-wrinkle essence of the present invention, the thickening agent includes sodium hyaluronate and carbomer, and the mass ratio of sodium hyaluronate to carbomer in the thickening agent is sodium hyaluronate:carbomer = 1.5:1.

[0023] As a preferred embodiment of the soothing anti-wrinkle essence of the present invention, the humectant in the soothing anti-wrinkle essence includes at least one of glycerol, butanediol and propylene glycol.

[0024] As a preferred embodiment of the soothing anti-wrinkle essence of the present invention, the humectant includes glycerol and butanediol, and the mass ratio of glycerol to butanediol in the humectant is glycerol:butanediol = (0.75 - 2.5):1.

[0025] As a preferred embodiment of the tea-peptide composition of the present invention, the humectant includes glycerol and butanediol, and the mass ratio of glycerol to butanediol in the humectant is glycerol:butanediol = (1.0 - 2.0):1.

[0026] As a preferred embodiment of the tea-peptide composition of the present invention, the humectant includes glycerol and butanediol, and the mass ratio of glycerol to butanediol in the humectant is glycerol:butanediol = 1.5:1.

[0027] As a preferred embodiment of the soothing anti-wrinkle essence of the present invention, the preservatives in the soothing anti-wrinkle essence include at least one of phenoxyethanol, 1,2-hexanediol, and p-hydroxyacetophenone.

[0028] Fourthly, the present invention provides a preparation method of the above-mentioned soothing anti-wrinkle essence, which includes the following steps:

[0029] (1) Mix the thickener and the humectant to obtain mixture A;

[0030] (2) Add the mixture A obtained in step (1) to water, and stir at 60 - 70°C and 300 - 500 rpm to obtain an aqueous phase;

[0031] (3) When the aqueous phase obtained in step (2) cools down to 40 - 50°C, add the above-mentioned tea-peptide composition, stir at 40 - 50°C and 150 - 250 rpm for 5 - 8 min, and perform homogenization treatment at 1500 - 2500 rpm for 3 - 4 min to obtain mixture B;

[0032] (4) When the mixture B obtained in step (3) cools down to 30 - 35°C, add the preservative, mix at 30 - 35°C and 80 - 150 rpm, and age to obtain the soothing anti-wrinkle essence.

[0033] As a preferred embodiment of the preparation method of the soothing anti-wrinkle essence of the present invention, in step (4), the aging is to seal and place at 23 - 27°C for 24 - 48 h.

[0034] Compared with the prior art, the beneficial effects of the present invention are:

[0035] Based on the anti-inflammatory mechanism of Keemun black tea fermentation filtrate and DNA sodium, as well as the anti-wrinkle function of decapeptide-4, the present invention realizes the synergistic effect of soothing-anti-wrinkle dual skin care. At the same time, the experimental research of the present invention shows that Keemun black tea fermentation filtrate and DNA sodium can synergistically improve the elastase inhibition rate of decapeptide-4. Conversely, decapeptide-4 can also synergistically improve the hyaluronidase inhibition rate of Keemun black tea fermentation filtrate and DNA sodium. The unique synergistic mechanism formed by the three enables the tea-peptide composition to have both soothing and anti-wrinkle effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 It is a product diagram of the tea-peptide composition obtained in Examples 1-7 of the present invention;

[0037] Figure 2 It is the detection result of the elastase inhibition rate of different tea-peptide compositions in Effect Example 2 of the present invention. DETAILED DESCRIPTION OF THE INVENTION

[0038] To better illustrate the purpose, technical solution and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0039] Other materials, reagents, etc. used in the examples, comparative examples and effect examples can be obtained from commercial channels without special instructions.

[0040] The Keemun black tea fermentation filtrate and Yue black tea fermentation filtrate in the following examples and comparative examples are all prepared according to Patent CN119605887A, and the Yue black tea fermentation filtrate is fermented by replacing Keemun black tea with Yuehong congou tea;

[0041] DNA sodium (also known as PDRN) is purchased from Shaanxi Baichanghong Technology Co., Ltd.;

[0042] Decapeptide-4 is purchased from Shenzhen Redlin Biotechnology Co., Ltd.;

[0043] Acetyl hexapeptide-8 is purchased from Dongguan Juwei New Material Technology Co., Ltd.

[0044] Examples 1-10 and Comparative Examples 1-7

[0045] Examples 1-10 and Comparative Examples 1-7 respectively provide a soothing and anti-wrinkle essence and its preparation method. The components and dosages of the soothing and anti-wrinkle essence are shown in Table 1, and the preparation method includes the following steps:

[0046] (1) Mix the thickener and the moisturizer to obtain mixture A;

[0047] (2) Add the mixture A obtained in step (1) to water and stir at 60-70 °C and 300-500 rpm to obtain an aqueous phase;

[0048] (3) When the aqueous phase obtained in step (2) is cooled to 40 - 50 °C, add the tea - peptide composition, stir at 40 - 50 °C and 150 - 250 rpm for 5 - 8 min, and homogenize at 1500 - 2500 rpm for 3 - 4 min to obtain mixture B;

[0049] (4) When the mixture B obtained in step (3) is cooled to 30 - 35 °C, add the preservative, mix at 30 - 35 °C and 80 - 150 rpm, seal and age at 23 - 27 °C for 24 - 48 h to obtain the soothing anti - wrinkle essence.

[0050] The morphologies of the soothing anti - wrinkle essences obtained in Examples 1 - 7 are shown in Figure 1 .

[0051] Table 1 Components and their dosages (parts by mass) of different soothing anti - wrinkle essences

[0052]

[0053]

[0054] Comparative Example 8

[0055] Comparative Example 8 provides a soothing anti - wrinkle essence and its preparation method. The components and their dosages of the soothing anti - wrinkle essence are similar to those of Example 2, except that the Qimen black tea fermentation filtrate in the tea - peptide composition is replaced with Yuehong tea fermentation filtrate, and the other components and their dosages remain unchanged. The preparation method is the same as that of Example 2.

[0056] Comparative Example 9

[0057] Comparative Example 9 provides a soothing anti - wrinkle essence and its preparation method. The components and their dosages of the soothing anti - wrinkle essence are similar to those of Example 2, except that the decapeptide - 4 in the tea - peptide composition is replaced with acetyl hexapeptide - 8, and the other components and their dosages remain unchanged.

[0058] Effect Example 1

[0059] Conduct an anti - inflammatory efficacy test on the soothing anti - wrinkle essences obtained in Examples 1 - 10 and Comparative Examples 1 - 9. The specific scheme is as follows:

[0060] Hyaluronidase is an endogenous hexosaminidase that causes the decomposition of large - molecule hyaluronic acid. Under the action of hyaluronidase, large - molecule hyaluronic acid decomposes to produce N - N - acetylglucosamine. Hyaluronidase can hydrolyze the 1,4 - glycosidic bond between β - N - N - acetylglucosamine and D - glucuronic acid in hyaluronic acid to obtain β - N - N - acetylglucosamine. β - N - N - acetylglucosamine can condense with acetylacetone under alkaline conditions to form a chromogen, 2 - methyl - 3 - acetylpyrrole derivative. The chromogen reacts with dimethylaminobenzaldehyde in concentrated hydrochloric acid - ethanol to produce color. Therefore, measuring the content of N - N - acetylglucosamine in the reaction system can indirectly reflect the activity of hyaluronidase. Hyaluronidase has a strong correlation with inflammation, so inhibiting the activity of hyaluronidase is used as an index for studying anti - inflammatory effects.

[0061] 1. Reagents and Materials

[0062] 1.1 Reagents: Acetic acid - sodium acetate buffer solution (pH = 5.6), sodium hyaluronate solution, Ehrlich's reagent, CaCl2 solution, NaOH solution, hyaluronidase solution, acetylacetone solution.

[0063] 1.2 Preparation of Reagents:

[0064] 1.2.1 Preparation of acetic acid - sodium acetate buffer solution (pH = 5.6):

[0065] Measure 1.155 mL of glacial acetic acid and dilute it to 100 mL and mix well to prepare acetic acid solution; weigh 2.72 g of sodium acetate, dissolve it in water and make up the volume to 100 mL to prepare sodium acetate solution; add 4.8 mL of acetic acid solution to 45.2 mL of sodium acetate solution, mix well and make up the volume to 100 mL to prepare acetic acid - sodium acetate buffer solution (pH = 5.6).

[0066] 1.2.2 Sodium hyaluronate solution: Weigh 0.0050 g of sodium hyaluronate and dissolve it in 10 mL of acetic acid - sodium acetate buffer solution (pH = 5.6) to prepare a sodium hyaluronate solution with a mass concentration of 0.5 mg / mL.

[0067] 1.2.3 Ehrlich's reagent: Weigh 0.8 g of p - dimethylaminobenzaldehyde and dissolve it in 15 mL of concentrated hydrochloric acid and 15 mL of absolute ethanol, and store it in the dark.

[0068] 1.2.4 CaCl2 solution: Weigh 2.8 g of anhydrous calcium chloride and dissolve it in 10 mL of deionized water, and sonicate until completely dissolved.

[0069] 1.2.5 NaOH solution: Weigh 0.16 g of NaOH and dissolve it in 10 mL of deionized water, and sonicate until completely dissolved.

[0070] 1.2.6 Hyaluronidase solution: Weigh 0.0100 g of hyaluronidase and dissolve it in 4 mL of acetic acid-sodium acetate buffer solution to prepare the hyaluronidase solution with a working concentration of 1250 U / mL. Prepare it before use.

[0071] 1.2.7 Acetylacetone solution: Measure 3.5 mL of acetylacetone and dissolve it in 50 mL of sodium carbonate solution (1.0 mol / L) to prepare the acetylacetone solution. Prepare it before use.

[0072] 2. Instruments and equipment: Analytical balance (accurate to 0.0001 g), 1 L volumetric flask, 50 mL measuring cylinder, 1000 μL pipette, constant temperature water bath, ultraviolet-visible spectrophotometer.

[0073] 3. Determination steps

[0074] Add the test samples and reagents to the test tubes in sequence according to Table 2. After the reaction, transfer them into a 3 cm cuvette and detect the absorbance at 547 nm, and record the data.

[0075] Table 2 Composition of the reaction solution (mL)

[0076]

[0077]

[0078] The absolute difference between the two independent determination results obtained under repeatability conditions shall not exceed 10% of the arithmetic mean. Calculate the hyaluronidase inhibition rate (%) according to formula (1), and the results are shown in Table 3.

[0079] Hyaluronidase inhibition rate (%) = [1 - (OD C - OD D ) / (OD A - OD B )] × 100% —— Formula (1)

[0080] In the above formula, OD A is the absorbance value of the enzyme solution control group; OD B is the absorbance value of the blank control group of the enzyme solution; OD C is the absorbance value of the sample group; OD D is the absorbance value of the blank control of the sample group.

[0081] Table 3 Detection results of hyaluronidase inhibition rate of different soothing and anti-wrinkle essence liquids

[0082]

[0083]

[0084] As shown in Table 3, the hyaluronidase inhibition rates of the soothing anti-wrinkle essence obtained in the examples are all higher than those in the comparative examples, indicating that the soothing anti-wrinkle essence containing the tea-peptide composition of the present invention has good anti-inflammatory effects, which also shows that the tea-peptide composition of the present invention has excellent soothing effects.

[0085] In Examples 1-5, as the dosage of Keemun black tea fermentation filtrate and decapeptide-4 increased, the hyaluronidase inhibition rate also increased; in Examples 2, 6, and 7, as the dosage of the tea-peptide composition increased, the hyaluronidase inhibition rate of the soothing anti-wrinkle essence also increased; in Comparative Examples 1-3, the sum of the hyaluronidase inhibition rates of the three in Comparative Examples 1-3 (16.27%) was lower than that in the examples; in Comparative Examples 8-9, when the Keemun black tea fermentation broth or decapeptide-4 was replaced with similar components, similar anti-inflammatory effects to those in the examples could not be obtained. The above results illustrate that the anti-inflammatory (soothing) effect of the tea-peptide composition is achieved based on the combination of Keemun black tea fermentation broth, sodium DNA, and decapeptide-4. If any one component is missing, any component is replaced with a similar component, or the ratio of each component is inappropriate, the anti-inflammatory (soothing) effect of the tea-peptide composition will be reduced. In addition, the present invention selects the Keemun black tea fermentation filtrate as one of the components of the tea-peptide composition because the Keemun black tea fermentation filtrate contains richer active ingredients than the Yue black tea fermentation filtrate, which can further enhance the anti-inflammatory (soothing) effect of the tea-peptide composition.

[0086] In Comparative Example 4, the hyaluronidase inhibition rates are lower than those in the examples and other comparative examples, which indicates that the main soothing component in the soothing anti-wrinkle essence is the tea-peptide composition. In addition to being prepared into a soothing anti-wrinkle essence, the tea-peptide composition of the present invention can also be prepared into different types of cosmetics such as water, milk, and cream as a cosmetic raw material with soothing effects, and has a wide range of applications.

[0087] Effect Example 2

[0088] The soothing anti-wrinkle essence obtained in Examples 1-10 and Comparative Examples 1-9 was subjected to a skin firmness test, and the specific scheme is as follows:

[0089] Collagen forms collagen fibers and reticular fibers, and elastin forms elastic fibers. These three types of fibers together form the connective tissue of the skin, constituting a plump and elastic structure of the skin. Elastase has the ability to decompose various proteins such as collagen and elastin, and the change of elastin structure is closely related to skin aging. The inhibition effect of the test sample on the catalysis of its substrate by elastase was detected using an enzyme-labeled instrument to determine whether the test sample has the ability to inhibit elastase.

[0090] 1. Reagents and materials

[0091] 1.1 30 U / mg elastase (porcine pancreatic protease), N-succinyl-alanine-alanine-alanine-p-nitroanilide, tris(hydroxymethyl)aminomethane (Tris), concentrated hydrochloric acid, and epigallocatechin gallate (EGCG).

[0092] 1.2 Preparation of 0.2 mol / L Tris-HCl buffer (buffer) with pH 8.0:

[0093] Solution A: Weigh accurately 4.8456 g of Tris granules, add 100 mL of deionized water, stir and mix well until dissolved to obtain a 0.4 mol / L Tris solution.

[0094] Solution B: Take 3.34 mL of concentrated hydrochloric acid and dilute it with 96.66 mL of deionized water to obtain a 0.4 mol / L HCl solution.

[0095] Take 100 mL of Solution A and 44.76 mL of Solution B, mix them, adjust the pH to 8.0, and add deionized water to make up the volume to 200 mL.

[0096] 1.3 Elastase solution (enzyme solution): Prepared with the buffer. Take 0.033 g of elastase, add 10 mL of the buffer, and prepare a 100 U / mL elastase solution, which is prepared immediately before use.

[0097] 1.4 Substrate solution: Weigh 1.8057 mg of N-succinyl-alanine-alanine-alanine-p-nitroanilide, dissolve it in 10 mL of the buffer to obtain a 0.18057 mg / mL N-succinyl-alanine-alanine-alanine-p-nitroanilide solution.

[0098] 2. Instruments and equipment

[0099] Analytical balance (accurate to 0.001 g), ultrasonic cleaner, pipette, pipette tube, colorimetric tube, and volumetric flask (10 mL and 100 mL).

[0100] 3. Measurement steps

[0101] Add each solution to the 96-well plate in sequence according to Table 4, incubate at room temperature for 60 min, measure the absorbance at 410 nm with an enzyme-labeled instrument, record and save the data. The absolute difference between two independent measurement results obtained under repeatability conditions shall not exceed 10% of the arithmetic mean. Calculate the elastase inhibition rate (%) according to formula (2), and the results are shown in Table 5.

[0102] Elastase inhibition rate (%) = [1 - (OD A - OD B ) / (OD C - OD D )] × 100% (Formula 2)

[0103] In the above formula, OD A is the absorbance value of the sample group (containing enzyme and substrate); OD B is the absorbance value of the sample control group (containing enzyme but no substrate); OD C is the absorbance value of the blank group (containing enzyme, substrate but no sample); OD D is the absorbance value of the blank control group (containing enzyme, no substrate and no sample).

[0104] Table 4 Composition of the test solution

[0105] Composition / (μL) Sample solution Enzyme solution Substrate solution Buffer solution Blank group / 50 100 50 Blank control group / 50 / 150 Sample group 50 50 100 / Sample control group 50 50 / 100

[0106] Table 5 Detection results of elastase inhibition rate of different soothing and anti-wrinkle essences

[0107] Group Elastase inhibition rate (%) Example 1 34.21±0.892 Example 2 50.23±3.314 Example 3 37.44±0.766 Example 4 46.66±1.881 Example 5 41.11±0.682 Example 6 65.55±0.124 Example 7 75.21±1.817 Example 8 58.88±2.192 Example 9 60.25±0.981 Example 10 55.91±2.577 Comparative example 1 6.21±0.597 Comparative example 2 2.23±2.984 Comparative example 3 8.11±2.655 Comparative example 4 9.23±2.236 Comparative example 5 12.64±0.972 Comparative example 6 13.79±0.244 Comparative example 7 14.21±3.545 Comparative example 8 21.23±5.877 Comparative example 9 18.99±3.458

[0108] As shown in Table 5, the elastase inhibition rates of the soothing and anti-wrinkle essence liquids of the examples are all higher than those of the comparative examples, indicating that the soothing and anti-wrinkle essence liquid containing the tea-peptide composition of the present invention has a good anti-wrinkle effect.

[0109] In Examples 1-5, as the dosage of Qimen black tea fermentation filtrate and decapeptide-4 increased, the elastase inhibition rate also increased; in Examples 2, 6, and 7, as the dosage of the tea-peptide composition increased, the elastase inhibition rate of the soothing and anti-wrinkle essence liquid also increased; in Comparative Examples 1-3, the sum of the elastase inhibition rates of the three in Comparative Examples 1-3 (16.55%) was lower than that of the examples; in Comparative Examples 8-9, replacing the Qimen black tea fermentation liquid or decapeptide-4 with similar components could not obtain an anti-wrinkle effect similar to that of the examples. The above results illustrate that the anti-wrinkle effect of the tea-peptide composition is achieved based on the Qimen black tea fermentation liquid, sodium DNA, and decapeptide-4. If one component is missing, any component is replaced with a similar component, or the ratio of each component is inappropriate, the anti-wrinkle effect of the tea-peptide composition will be reduced. In addition, the present invention selects the Qimen black tea fermentation filtrate as one of the components of the tea-peptide composition because the Qimen black tea fermentation filtrate contains richer active ingredients than the Yuehong tea fermentation filtrate, which can further enhance the anti-wrinkle effect of the tea-peptide composition.

[0110] In Comparative Example 4, the hyaluronidase inhibition rates were lower than those of the examples and other comparative examples, which indicates that the main anti-wrinkle component in the soothing and anti-wrinkle essence liquid is the tea-peptide composition. The tea-peptide composition of the present invention can be prepared not only into a soothing and anti-wrinkle essence liquid but also into different types of cosmetics such as water, lotion, and cream as a cosmetic raw material with anti-wrinkle effects, and has a wide range of applications.

[0111] In summary, the Keemun black tea fermentation broth and DNA in the tea-peptide composition prepared by the present invention can synergistically enhance the anti-wrinkle efficacy of decapeptide-4, and decapeptide-4 can also synergistically enhance the soothing efficacy of the Keemun black tea fermentation filtrate and DNA. The three interact with each other to synergistically enhance their dual soothing and anti-wrinkle effects. When combined with various active ingredients and excipients of cosmetics, they can be prepared into cosmetics of different dosage forms with soothing and anti-wrinkle effects.

[0112] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit the protection scope of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A tea-peptide composition with soothing and anti-wrinkle effects, characterized in that, It comprises Keemun black tea fermentation filtrate, sodium DNA and decapeptide-4, and the mass ratio of the Keemun black tea fermentation filtrate, sodium DNA and decapeptide-4 is Keemun black tea fermentation filtrate:sodium DNA:decapeptide-4 = (500 - 10000):1:(1 - 20).

2. The tea-peptide composition according to claim 1, wherein In the tea-peptide composition, the mass ratio of the Keemun black tea fermentation filtrate, sodium DNA and decapeptide-4 is Keemun black tea fermentation filtrate:sodium DNA:decapeptide-4 = (1000 - 5000):1:(2 - 10).

3. Use of the tea-peptide composition according to claim 1 or 2 in the preparation of cosmetics.

4. A soothing anti-wrinkle essence, characterized in that, It comprises the following components in parts by mass: 5 - 11 parts of the tea-peptide composition according to any one of claims 1 - 2, 0.2 - 0.6 part of a thickener, 4 - 10 parts of a humectant, 0.2 - 0.6 part of a preservative, and 75 - 95 parts of water.

5. The soothing anti-wrinkle essence according to claim 4, wherein The thickener in the soothing anti-wrinkle essence includes at least one of sodium hyaluronate, carbomer and sodium alginate.

6. The soothing anti-wrinkle essence according to claim 5, characterized in that, The thickener includes sodium hyaluronate and carbomer, and the mass ratio of sodium hyaluronate to carbomer in the thickener is sodium hyaluronate:carbomer = (0.5 - 3):

1.

7. The soothing anti-wrinkle essence according to claim 4, wherein The humectant in the soothing anti-wrinkle essence includes at least one of glycerol, butanediol and propylene glycol.

8. The soothing anti-wrinkle essence according to claim 7, wherein The humectant includes glycerol and butanediol, and the mass ratio of glycerol to butanediol in the humectant is glycerol:butanediol = (0.75 - 2.5):

1.

9. The soothing anti-wrinkle essence according to claim 4, wherein The preservative in the soothing anti-wrinkle essence includes at least one of phenoxyethanol, 1,2-hexanediol and p-hydroxyacetophenone.

10. The preparation method of the soothing anti-wrinkle essence according to any one of claims 5-9, characterized in that, It comprises the following steps: (1) Mix the thickener and the humectant to obtain mixture A; (2) Add the mixture A obtained in step (1) to water, and stir at 60 - 70 °C and 300 - 500 rpm to obtain an aqueous phase; (3) When the aqueous phase obtained in step (2) cools to 40 - 50 °C, add the tea-peptide composition according to any one of claims 1 - 2, stir at 40 - 50 °C and 150 - 250 rpm for 5 - 8 min, and perform homogenization treatment at 1500 - 2500 rpm for 3 - 4 min to obtain mixture B; (4) When the mixture B obtained in step (3) cools to 30 - 35 °C, add the preservative, and mix and age at 30 - 35 °C and 80 - 150 rpm to obtain the soothing anti-wrinkle essence.

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